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Id: biblio-1011418
Autor: Mohaqiq, Mahdi; Movahedin, Mansoureh; Mazaheri, Zohreh; Amirjannati, Naser.
Título: In vitro transplantation of spermatogonial stem cells isolated from human frozen-thawed testis tissue can induce spermatogenesis under 3-dimensional tissue culture conditions
Fonte: Biol. Res;52:16, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Sperm production is one of the most complex biological processes in the body. In vitro production of sperm is one of the most important goals of researches in the field of male infertility treatment, which is very important in male cancer patients treated with gonadotoxic methods and drugs. In this study, we examine the progression of spermatogenesis after transplantation of spermatogonial stem cells under conditions of testicular tissue culture. RESULTS: Testicular tissue samples from azoospermic patients were obtained and then these were freeze-thawed. Spermatogonial stem cells were isolated by two enzymatic digestion steps and the identification of these cells was confirmed by detecting the PLZF protein. These cells, after being labeled with DiI, were transplanted in azoospermia adult mice model. The host testes were placed on agarose gel as tissue culture system. After 8 weeks, histomorphometric, immunohistochemical and molecular studies were performed. The results of histomorphometric studies showed that the mean number of spermatogonial cells, spermatocytes and spermatids in the experimental group was significantly more than the control group (without transplantation) (P < 0.05) and most of the cells responded positively to the detection of DiI. Immunohistochemical studies in host testes fragments in the experimental group express the PLZF, SCP3 and ACRBP proteins in spermatogonial cells, spermatocyte and spermatozoa, respectively, which confirmed the human nature of these cells. Also, in molecular studies of PLZF, Tekt1 and TP1, the results indicated that the genes were positive in the test group, while not in the control group. CONCLUSION: These results suggest that the slow freezing of SSCs can support the induction of spermatogenesis to produce haploid cells under the 3-dimensional testicular tissue culture.
Descritores: Espermatogênese/fisiologia
Espermatogônias/transplante
Testículo/citologia
Criopreservação/métodos
Transplante de Células-Tronco/métodos
Limites: Humanos
Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-553338
Autor: Parmigiani, Raphael Bessa.
Título: Caracterização de um novo antígeno tumoral: CTSP-1 / Characterization of a new tumor antigen: CTSP-1.
Fonte: São Paulo; s.n; 2005. 140 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: A necessidade de identificar novos antígenos tumorais que possam ser utilizados no tratamento e diagnóstico do câncer, tem levado ao desenvolvimento de técnicas cada vez mais eficientes na detecção dos mesmos. Antígenos de diferentes categorias foram identificados e caracterizados, sendo os antígenos cancer-testis (CT) e os antígenos de diferenciação (CD) os de maior importância clínica, dado seu restrito padrão de expressão. Utilizando uma estratégia de alinhamento de seqüências expressas no genoma humano, identificamos um novo transcrito localizado no cromossomo 21, denominado CTSP-1, que apresenta alta similaridade com o antígeno tumoral NY-BR-1... Além disso, avaliamos seu padrão de expressão em diferentes tecidos normais, linhagens celulares tumorais e amostras de tumores de pacientes... Através de immunoblotting foi possível a identificação de uma banda específica de 22kDa, correspondente ao peso esperado da proteína CTSP-1 em extrato total de testículo normal. Em seguida, através da imunohistoquímica, verificamos uma marcação preferencial nas espermatogônias e células de Leydig de testículo normal. Nos tecidos com amostras pareadas normal/tumor (mama e próstata), apenas as amostras tumorais foram fortemente marcadas. Posteriormente, a proteína CTSP-1 recombinante foi utilizada na investigação de anticorpos específicos em plasma de pacientes com câncer. Aproximadamente 150 amostras foram analisadas, das quais 20% apresentaram resposta imune humoral contra a proteína CTSP-1. Estes resultados revelam que o CTSP-1 é um novo antígeno tumoral da categoria dos CTs, com expressão restrita a tumor e testículo e com alta imunogenicidade em pacientes com câncer...(AU)

The need to identify new tumor antigens to be used in cancer treatment and diagnosis has lead to the development of efficient techniques for this purpose. Antigens from different categories have been identified and characterized and, among those, the cancer-testis (CT) and the cancer differentiation (CD) antigens are of the greatest clinicai interest dueto their restricted expression partem. Using alignments between expressed sequences and the Human Genome Sequence, we identified a new gene located on chromosome 21, named CTSP-1. This gene has a high similarity to the tumor antigen NY-BR-1, which encodes for a tissue specific transcription factor and is a potential target for cancer immunotherapy. In order to verify ifthe CTSP-1 gene is really a new tumor antigen, we performed its complete characterization. Using different techniques, we were able to obtain the complete sequence of the CTSP-1 gene and to identify different alternative polyadenilation and splicing forms. Moreover, we analyzed the CTSP-1 expression pattern in normal tissues, tumor cell lines and tumor samples. CTSP-1 showed a restricted expression pattern, being expressed only in testis among normal tissues, in different tumor celllines (9/22) and in different tumor types (7 4/178), which matches with the expression pattern of Cancer-Testis antigens. Afterwards, the recombinant CTSP-1 protein was expressed in a heterologous system and used for the generation of polyclonal antibody in mice. This antiboby was used in immunoblotting and immunohistochemistry experiments for the detection of CTSP-1 protein in normal testis and paired normal and tumor samples from breast and prostate. Using immunoblotting, a 22kDa specific band was identified in testis total protein extract, corresponding to the expected molecular weight of the CTSP-1 protein. Using immunohistochemistry, we verified the preferential staining of germ cells and Leydig cells in normal testis. Among tissues with paired normal/tumor samples, only tumor samples were strongly stained. The CTSP-1 recombinant protein was also used in the search for specific antibodies in plasma from cancer patients. Approximately 150 samples were analyzed, of which 20% showed a humoral immune response against the CTSP-1 protein. Taken together, these results confirm that the CTSP-1 gene is a new tumor antigen from the Cancer-Testis category, with restricted expression in testis and tumors and with high immunogenicity in cancer patients (AU)
Descritores: Antígenos
Bancos de Espécimes Biológicos
Immunoblotting
Imunoterapia
Poliadenilação
Processamento Alternativo
-Imuno-Histoquímica
Células Intersticiais do Testículo
Espermatogônias
Limites: Humanos
Masculino
Responsável: BR30.1 - Biblioteca
BR30.1


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Id: biblio-1012409
Autor: Talebi, Ali; Sadighi-Gilani, Mohammad Ali; Koruji, Morteza; Ai, Jafar; Navid, Shadan; Rezaie, Mohammad Jafar; Jabari, Ayob; Ashouri-Movassagh, Sepideh; Khadivi, Farnaz; Salehi, Majid; Hoshino, Yumi; Abbasi, Mehdi.
Título: Proliferation and differentiation of mouse spermatogonial stem cells on a three-dimensional surface composed of PCL/gel nanofibers / Proliferación y diferenciación de células madre espermatogónicas de ratón en una superficie tridimensional compuesta de nanofibras PCL / gel
Fonte: Int. j. morphol;37(3):1132-1141, Sept. 2019. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.

Las células madre espermatogónicas (CME) tienen capacidad de auto renovación y diferenciación esenciales para la producción de esperma a lo largo de la vida reproductiva masculina. El «scaffold¼ nanofibroso de policaprolactona / gelatina (PCL / Gel) electrohilado imita características importantes de la matriz extracelular (MEC), que puede proporcionar una técnica prometedora para la proliferación y diferenciación de CME in vitro. El objetivo del presente estudio fue investigar los efectos del «scaffold¼ nanofibroso PCL / Gel en la propagación y diferenciación de CME de ratones neonatos (mSSC). Los mSSC se aislaron enzimáticamente y las células se purificaron mediante un método de siembra diferencial y se sembraron en un «scaffold¼. Después de 2 semanas, se investigaron la viabilidad, el número y el diámetro de las colonias y la expresión de genes específicos de células espermatogónicas. Después de la propagación de mSSC, las células se cultivaron en un medio de diferenciación en el «scaffold¼ durante otras 2 semanas, y las células se analizaron mediante PCR en tiempo real. El número de colonias mSSC (P <0,01) y los niveles de expresión de los genes espermatogónicos específicos Plzf e Inga6 (P <0,01) y también los genes de diferenciación c-Kit, Tp1 y Ptm1 (P <0,05) fueron mayores en el grupo de «scaffold¼ en comparación con el control durante el período de cultivo. Concluimos que los mSSC pueden expandirse y diferenciarse en células espermátidas en un «scaffold¼ de nanofibras PCL / Gel con parámetros de desarrollo mejorados.
Descritores: Espermatogônias/citologia
Espermatogônias/metabolismo
Diferenciação Celular/fisiologia
Proliferação de Células/fisiologia
-Poliésteres/química
Diferenciação Celular/genética
Sobrevivência Celular
Imunofluorescência
Proliferação de Células/genética
Tecidos Suporte
Nanofibras/química
Reação em Cadeia da Polimerase em Tempo Real
Animais Recém-Nascidos
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-668049
Autor: Aponte, Pedro Manuel; Schlatt, Stefan; Franca, Luiz Renato de.
Título: Biotechnological approaches to the treatment of aspermatogenic men
Fonte: Clinics;68(supl.1):157-167, 2013. ilus, tab.
Idioma: en.
Resumo: Aspermatogenesis is a severe impairment of spermatogenesis in which germ cells are completely lacking or present in an immature form, which results in sterility in approximately 25% of patients. Because assisted reproduction techniques require mature germ cells, biotechnology is a valuable tool for rescuing fertility while maintaining biological fatherhood. However, this process involves, for instance, the differentiation of preexisting immature germ cells or the production/derivation of sperm from somatic cells. This review critically addresses four potential techniques: sperm derivation in vitro, germ stem cell transplantation, xenologous systems, and haploidization. Sperm derivation in vitro is already feasible in fish and mammals through organ culture or 3D systems, and it is very useful in conditions of germ cell arrest or in type II Sertoli-cell-only syndrome. Patients afflicted by type I Sertoli-cell-only syndrome could also benefit from gamete derivation from induced pluripotent stem cells of somatic origin, and human haploid-like cells have already been obtained by using this novel methodology. In the absence of alternative strategies to generate sperm in vitro, in germ cells transplantation fertility is restored by placing donor cells in the recipient germ-cell-free seminiferous epithelium, which has proven effective in conditions of spermatogonial arrest. Grafting also provides an approach for ex-vivo generation of mature sperm, particularly using prepubertal testis tissue. Although less feasible, haploidization is an option for creating gametes based on biological cloning technology. In conclusion, the aforementioned promising techniques remain largely experimental and still require extensive research, which should address, among other concerns, ethical and biosafety issues, such as gamete epigenetic status, ploidy, and chromatin integrity.
Descritores: Tecnologia Biomédica/métodos
Infertilidade Masculina/terapia
Técnicas de Reprodução Assistida
-Preservação da Fertilidade/métodos
Espermatogênese
Espermatogônias/transplante
Transplante de Células-Tronco/métodos
Transplante Heterólogo
Limites: Animais
Humanos
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Id: lil-501483
Autor: Rendón Rodríguez, Sergio; Macías Regalado, Emilio; Calderón Pérez, José Antonio; Núñez Pastén, Arturo; Solís Ibarra, Rafael.
Título: Comparison of some reproductive characteristics of farmed and wild white shrimp males Litopenaeus vannamei (Decapoda: Penaeidae)
Fonte: Rev. biol. trop;55(1):199-206, Mar. 2007. tab, graf.
Idioma: en.
Resumo: We rated some reproductive characteristics of white shrimp Litopenaeus vannamei (Boone, 1931) males using 46 farmed individuals (weighing 21.42 +/- 0.56 g) and 40 wild individuals (weighing 36.10 +/- 0.72 g). In farmed shrimps, spermatophore mean weight was 8.94 +/- 0.51 mg; total mean sperm count was 3.90 +/- 0.27 x 10(6) in each spermatophore; and mean percentage of normal sperm was 86.9 +/- 0.37%. In wild individuals, the respective values were 30.68 +/- 2.32 mg; 6.22 +/- 1.09 x 10(6); and 62.1 +/- 3.56%. In both groups, the differences between right and left spermatophore were not significant (p < 0.01). There were significant differences in spermatophore weight and percentage of normal sperm between farmed and wild shrimps; sperm counts differences, however, were not significant (p < 0.01). The relationship between spermatophore weight (Ws) and individual weight (Wo) was Ws (mg)=1.23 (Wo)-17.34 (r2=0.89), in farmed shrimps; and Ws (mg) = 2.57 (Wo)-60.04 (r2 = 0.64), in wild ones. In cultivated organisms, the relationship between sperm counts (Cs) and individual weight (Wo) was Cs (x 10(6)) = 1.13 * 10(-4*) (Wo) 3.361 (r2 = 0.85); and versus spermatophores weight was Cs (x 10(6)) = 0.439* (Ws) 0.984 (r2 = 0.90). In wild organisms, there was no correlation. The proportion of normal sperm ranged from 79.8 to 95.2 % (86.9 +/- 0.37%) and from 14.0 to 91.5% (62.1 +/- 2.52%), in farmed and wild shrimps, respectively. The most frequent abnormalities in both farm and wild animals were sperm without spike (49.3% and 76.6%, respectively) and irregular shape (35.8 % and 17.7 %). The less frequent occurrences were those of bent (10.2 % and 4.29%) and double spike (4.7% and 1.41%).
Descritores: Espermatogônias/crescimento & desenvolvimento
Penaeidae/fisiologia
Contagem de Espermatozoides
-Animais Selvagens
Aquicultura
Penaeidae/anatomia & histologia
Reprodução/fisiologia
Limites: Animais
Masculino
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-495117
Autor: Nakayama, Cintia; Peixoto, Silvio; Lopes, Diogo; Vita, Gustavo; Krummenauer, Dariano; Foes, Geraldo; Cavalli, Ronaldo; Wasielesky, Wilson.
Título: Métodos de extrusão manual e elétrica dos espermatóforos de reprodutores selvagens do camarão-rosa Farfantepenaeus paulensis (Decapoda: Penaeidae) / Manual and electrical spermatophore extrusion methods of the pink shrimp Farfantepenaeus paulensis (Decapoda: Penaeidae) wild broodstock
Fonte: Ciênc. rural;38(7):2018-2022, out. 2008. tab.
Idioma: pt.
Resumo: O presente estudo comparou os métodos manual e elétrico de extrusão do espermatóforo do camarão-rosa Farfantepenaeus paulensis com o objetivo de verificar se os métodos de extrusão exercem influência na quantidade de células espermáticas e na regeneração de novos espermatóforos. Os machos foram extrusados no início (dia zero) e no final do experimento (dia 43) para verificação da eficiência dos métodos no processo de regeneração. A extrusão manual foi realizada por meio de pressão na região do quinto par de pereiópodos e o método elétrico com uso de eletrodo para transmissão de impulso elétrico de 9 volts na mesma região. Os dois métodos foram considerados eficientes, não sendo encontradas diferenças significativas entre estes (P>0,05) para o número de células espermáticas. Entretanto, foi verificada, no final do experimento, a perda de peso corporal, peso de espermatóforo e menor índice espermatossomático (IES) (P<0,05) nos animais submetidos a extrusão do espermatóforo por estímulo elétrico. Os valores médios finais (±DP) do número de espermatozóides foram 1,46 (±0,84) e 3,25 milhões (±2,12) para os tratamentos com método elétrico e manual, respectivamente. Os resultados indicam que ambos os métodos podem ser utilizados para a coleta inicial de espermatóforos e testes de qualidade de espermatozóide. Entretanto, para a reutilização dos machos após a extrusão inicial do espermatóforo, o método manual é mais indicado pela manutenção do número de células espermáticas, peso do espermatóforo, peso corporal e índice espermatossomático após a regeneração.

The present study compared manual and electrical methods to extrude the spermatophore of the pink shrimp Farfantepenaeus paulensis aiming to analyze their influence on the number of spermatic cells and spermatophore regeneration. The males were extruded in the beginning (day zero) and in the end (43rd day) of the experiment to evaluate the efficiency of these methods in the regeneration process. For the extrusion, a gentle pressure was applied manually in the fifth pair of pereiopods or electrically by a 9 volt pulse in the same area. Both methods were efficient in removing the spermatophore and no significant differences were found (P>0.05) in the number of sperm cells. Nevertheless, significant decreases (P<0.05) in the body weight, spermatophore weight and spermatosomatic index (ESI) at the end of the experimental period were observed by using the electrical stimulation. The mean values (±SD) of the number of sperm cells were 1.46 (±0.84) and 3.25 (±2.12) millions for the electrical and manual treatments, respectively. Results indicate that both methods may be applied to collect initial samples of spermatophores as well as for sperm quality testing. However, when previously spermatophore-extruded males are to be used, the manual method is indicated as the number of spermatic cells, spermatophore weight, body weight, and ESI are maintained after regeneration.
Descritores: Penaeidae
Espermatogônias
-Inseminação Artificial/veterinária
Limites: Animais
Masculino
Responsável: BR409.1 - Biblioteca


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Id: lil-489591
Autor: Hadziselimovic, Faruk.
Título: Successful treatment of unilateral cryptorchid boys risking infertility with LH-RH analogue
Fonte: Int. braz. j. urol;34(3):319-328, May-June 2008. graf, tab.
Idioma: en.
Resumo: INTRODUCTION: Infertility is the primary concern for boys with uni- or bilateral undescended testes. An early and seemingly successful orchiopexy does not improve fertility in a substantial number of cryptorchid males. We confirmed that LH-RH analogue (LH-RHa) treatment induces an increase in and maturation of the germ cells; however, it was uncertain if treatment would improve the chance of fertility later in life. MATERIALS AND METHODS: Thirty unilateral cryptorchid boys, with an average age of 3 years at the time of surgery, were included in the study. Testicular biopsy showed that they had impaired testicular maturation and were therefore at high risk for infertility. Fifteen of the 30 unilateral cryptorchid boys were treated with 10 µg LH-RHa (Buserelin) nasal spray, administered on alternate days for a period of 6 months, following orchiopexy. The control group consisted of 15 cryptorchid boys who had been treated by Schoemakers type of orchiopexy, alone. After puberty, the ejaculates of both groups were analyzed. RESULTS: All males in the untreated group were severely oligospermic, with 20 percent being azoospermic. In contrast, 86 percent of the treated ex-cryptorchid males had a sperm concentration within the normal range; this was significantly different from the sperm concentration found in the untreated group (p = 0.000008). CONCLUSION: For the first time, we demonstrate that infertility in cryptorchidism can be successfully corrected when suitably treated with a LH-RHa. Sperm parameters normalized following therapy in the majority of cryptorchid males who, untreated, would have remained infertile. This innovative hormonal treatment will have a profound effect on the current recommended surgical treatment of boys with undescended testes.
Descritores: Busserrelina/administração & dosagem
Criptorquidismo/tratamento farmacológico
Hormônio Liberador de Gonadotropina/análogos & derivados
Infertilidade Masculina/prevenção & controle
Contagem de Espermatozoides
-Administração Intranasal
Biópsia
Criptorquidismo/complicações
Criptorquidismo/cirurgia
Oligospermia/prevenção & controle
Espermatogônias
Testículo/patologia
Testículo/cirurgia
Procedimentos Cirúrgicos Urológicos Masculinos
Limites: Criança
Pré-Escolar
Humanos
Lactente
Masculino
Tipo de Publ: Ensaio Clínico
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-473824
Autor: Cuartas, Elena I; Petriella, A. M.
Título: Formación inicial de los espermatofóros en el testículo del cangrejo Uca uruguayensis (Brachyura: Ocypodidae) / Initial formation of spermatophores in the testicles of the crab Uca uruguayensis (Brachyura: Ocypodidae)
Fonte: Rev. biol. trop;55(supl.1):9-14, jun. 2007. ilus.
Idioma: es.
Conferência: Apresentado em: Congreso de Ciencias del Mar de Chile, 25, Apresentado em: Congreso Latinoamericano de Ciencias del Mar, 11, Viña del Mar, 16-20 mayo 2005.
Resumo: The functional anatomy of the male reproductive system of Uca uruguayensis from Mar Chiquita lagoon, (37º45' S, 57º26' W), Argentina, was known only from optical icroscopy. The present study describes the participation of vas deferens regions in spermatophore formation. A detailed description of the functional morphology of the different regions of the testicular lobes was carried out using both light and scanning electron microscopy. Spermatophore formation begins at the base of the testicular lobe. In most brachyuran species, the spermatophore starts formation when spermatozoa move from the collecting ducts of the testis to the vas deferens. However, in U. uruguayensis observations suggest that the formation of the spermatophore walls occurred in the terminal region of the testis, and that the spermatophore was formed at the junction of the testis and the vas deferens.

La anatomía funcional del sistema reproductor de los machos de Uca uruguayensis de la población de la laguna de Mar Chiquita (37º45' S, 57º26' W), Argentina, ha sido previamente estudiada empleando microscopía óptica. En el presente estudio se demostró la intervención del vaso deferente, en sus distintas regiones, en la formación del espermatóforo y la inclusión del fluido espermático. Se amplía la descripción de la morfología funcional de las regiones de los lóbulos testiculares (empleando también microscopía electrónica de barrido). La formación de los espermatóforos se inicia en la base del lóbulo testicular. El mecanismo descrito hasta el momento para la mayor parte de las especies de braquiuros postula que los espermatóforos comienzan a formarse cuando los espermatozoos pasan de los colectores del testículo al vaso deferente. Nuestras observaciones sugieren sin embargo, que en esta especie la formación de la pared del espermatóforo se inicia en la base de los lóbulos testiculares, y que los espermatóforos están completamente formados en la unión de los testículos y el vaso deferente anterior.
Descritores: Braquiúros/fisiologia
Espermatogônias/fisiologia
Testículo/fisiologia
-Braquiúros/anatomia & histologia
Microscopia Eletrônica de Varredura
Reprodução/fisiologia
Testículo/ultraestrutura
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-439391
Autor: Hadziselimovic, Faruk.
Título: Early successful orchidopexy does not prevent from developing azoospermia
Fonte: Int. braz. j. urol;32(5):570-573, Sept.-Oct. 2006.
Idioma: en.
Resumo: INTRODUCTION: The incidence of Ad spermatogonia (stem cells for fertility) was assessed in 20 cryptorchid patients, all of whom had a successful orchidopexy in childhood but developed azoospermia following puberty. MATERIALS AND METHODS: From a cohort of 231 patients who had a semen analysis following successful orchidopexy 20 patients (9 percent) had azoospermia. The patients were classified into 2 groups according to the time of surgery: A = < 21 months of age (n = 5, mean = 10.7 ± 8.6 months) and B = during childhood (n = 15, mean = 10.1 ± 3 years). Nine of the 20 patients (45 percent) had bilateral cryptorchidism: A = 1 and B = 8. Testicular biopsies were performed during orchidopexy and analyzed with semi-thin technique. The number of Ad spermatogonia and entire number of germ cells was determined. The patients' semen analyses were evaluated at least twice; FSH and testosterone plasma values were estimated. RESULTS: In group A, all patients had germ cells at the time of surgery (mean = 1.04 ± 1.4 germ cells per tubular cross section); only 6 patients in group B (40 percent) had no germ cells (mean = 0.17 ± 0.4); A vs. B, p = 0.0133. Importantly, Ad spermatogonia were absent in the entire study population. The plasma FSH of 16 patients (80 percent) was abnormal [median = 16.35 IU/L (Interquartile range of sample - IQR 9.075-27.85 95 percent CI, 3-53)] while the plasma testosterone of all the patients was normal. CONCLUSIONS: The most severe cause of infertility in cryptorchid patients cannot be mitigated by an early successful surgery alone.
Descritores: Azoospermia/etiologia
Criptorquidismo/cirurgia
Hormônio Foliculoestimulante/sangue
Orquiectomia
Espermatogônias
Testosterona/sangue
-Azoospermia/sangue
Azoospermia/diagnóstico
Estudos de Coortes
Criptorquidismo/sangue
Criptorquidismo/complicações
Contagem de Espermatozoides
Limites: Humanos
Masculino
Adolescente
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Cruz-Landim, C
Texto completo
Id: lil-393520
Autor: Abdalla, F. C; Cruz-Landim, C.
Título: Occurrence and ultrastructural characterization of "nuage" during oogenesis and early spermatogenesis of Piaractus mesopotamicus Holmberg, 1887 (Teleostei)
Fonte: Braz. j. biol;64(3b):555-561, ago. 2004. ilus.
Idioma: en.
Resumo: Foi investigada a ocorrência e caracterizada ultra-estruturalmente a eliminação de material nuclear eletrondenso (nuage) para o citoplasma durante a ovogênese e durante os estágios iniciais da espermatogênese de Piaractus mesopotamicus, um peixe do Pantanal Matogrossense de ciclo reprodutivo sazonal. Constataram-se nas células germinativas femininas dois momentos de eliminação desse material, na ovogônia e no ovócito em fase perinucleolar. Nas células masculinas, material com morfologia e comportamento muito semelhante foi encontrado na espermatogônia. Em todos os casos, o material associou-se a mitocôndrias. A possível função desse material foi discutida.
Descritores: Peixes
Oogênese
Espermatogênese
Espermatogônias
-Citoplasma
Microscopia Eletrônica
Mitocôndrias
Óvulo
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME



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