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Id: biblio-1121008
Autor: Castañeda, Rodrigo; Ortiz, Emily; Aldana, Caroline; Cruz, Sully M; Cáceres, Armando.
Título: Biomarcadores traslacionales de modelos in vitro e in vivo de daño renal: Una perspectiva para abordar nefrotoxicidad desde múltiples factores etiológicos / Biomarcadores traslacionales de modelos in vitro e in vivo de daño renal: Una perspectiva para abordar nefrotoxicidad desde múltiples factores etiológicos
Fonte: Cienc. tecnol. salud;7(1), 2020. ilus.
Idioma: es.
Resumo: La prevalencia de enfermedad renal ha aumentado considerablemente en la última década y está previsto que crezca en los próximos años. Recientemente, diversos modelos se han utilizado para entender los procesos fisiopatológicos de daño renal y para la búsqueda de futuros candidatos farmacológicos. El objetivo de esta revisión es proporcionar una descripción de la evidencia actual de modelos in vitro e in vivo de nefrotoxicidad, nefropatía diabética y deshidratación, y los fundamentos de las principales vías de señalización fisiopatológicas, con el fin de proponer biomarcadores candidatos para futura investigación farmacológica. Actualmente, los roedores constituyen un pilar importante en estudios de daño renal, existiendo diferencias específicas según el estímulo nocivo, lo que sugiere considerar para un modelo relevante aspectos como especie, cepa, género y estructuras renales objetivo. Diversas estructuras renales se han complementado in vitro, principalmente a partir de líneas celulares, como del epitelio tubular, podocitos, células mesangiales glomerulares y conducto colector medular interno. Este enfoque se ha utilizado como complementario en modelos de nefrotoxicidad por exposición a aminoglucósidos (principalmente), deshidratación por cloruro de sodio hiperosmolar, y nefropatía diabética por medio de glucosa alta y productos derivados de glucólisis y glicación. Recientemente, estos modelos han mostrado similitud en diversas rutas de señalización celular, con algunos biomarcadores en común, entre múltiples causas de daño renal como el daño oxidativo, disfunción mitocondrial, procesos inflamatorios, desregulación de sistemas de defensa y sobrevivencia celular, y apoptosis. El enfoque en seleccionar biomarcadores relevantes contribuirá al diseño de estrategias terapéuticas de nefroprotectores sobre múltiples factores etiológicos.

The prevalence of kidney disease has increased considerably in the last decade and is expected to growth in the coming years. Recently, various models have been used to understand the pathophysiological processes of kidney damage and to search for future pharmacological candidates. The aim of this review is to provide a description of the current evidence of in vitro and in vivo models of nephrotoxicity, diabetic nephropathy and dehydration, and the foundations of the main pathophysiological signaling pathways, in order to propose candidate biomarkers for future drug discovery. Currently, rodents are an important pillar in studies of kidney damage, with specific differences depending on the noxious stimulus, which suggests considering aspects such as species, strain, gender and target structures for a relevant model. Several renal structures have been complemented through in vitro approaches, mainly using cell lines, such as the tubular epithelium, podocytes, glomerular mesangial cells and inner medullary collecting duct. These cells have been used as models of nephrotoxicity by exposure to aminoglycosides (mainly), dehydration by exposure to hyperosmolar sodium chloride, and diabetic nephropathy by exposure to high glucose and products derived from glycolysis and glycation. Recently, these models have shown common cell signaling pathways on multiple etiologies of kidney injury, sharing several biomarkers such as oxidative damage, mitochondrial dysfunction, inflammatory processes, dysregulation of defense systems and cell survival, and apoptosis. Approaching kidney injury based on the selection of relevant biomarkers will contribute to the design of therapeutic strategies for nephroprotection on multiple etiological factors.
Descritores: Técnicas In Vitro/métodos
Biomarcadores
Nefropatias Diabéticas
-Roedores
Ratos Wistar
Apoptose
Epitélio
Células Mesangiais
Glucose/análise
Limites: Humanos
Animais
Masculino
Adolescente
Adulto
Ratos
Tipo de Publ: Revisão
Responsável: GT49.1


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Texto completo SciELO Chile
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Id: biblio-950844
Autor: Geng, Wenjia; Wei, Ribao; Liu, Shuwen; Tang, Li; Zhu, Hanyu; Chen, Pu; Wu, Jie; Zhang, Xueguang; Zhu, Fei; Yin, Zhong; Chen, Xiangmei.
Título: Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
Fonte: Biol. Res;49:1-10, 2016. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . China Postdoctoral Science Foundation; . National Natural Science Foundation of China.
Resumo: BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH). RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex. CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1.
Descritores: Medicamentos de Ervas Chinesas/farmacologia
Glomerulonefrite Membranoproliferativa/tratamento farmacológico
Proliferação de Células/efeitos dos fármacos
Células Mesangiais/efeitos dos fármacos
Isoanticorpos
-Fatores de Tempo
Albumina Sérica/análise
Medicamentos de Ervas Chinesas/uso terapêutico
Glomerulonefrite Membranoproliferativa/patologia
Doença Crônica
Reprodutibilidade dos Testes
Ratos Wistar
Proteína Quinase 1 Ativada por Mitógeno/análise
Ciclina D1/análise
Computadores de Mão
Quinases Ativadas por p21/análise
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-960236
Autor: Benavides, Mario Fernando; Fernández-Avila, Daniel Gerardo; Gutiérrez, Juan Martín.
Título: Male patient with C1q nephropathy and rheumatoid arthritis: A case report / Hombre con nefropatía C1q y artritis reumatoide: reporte de un caso
Fonte: Rev. colomb. reumatol;24(4):247-250, Oct.-Dec. 2017. graf.
Idioma: en.
Resumo: Abstract C1q nephropathy was first described in 1985 as a process of glomerulonephritis with mesangial C1q deposit. The histology is similar to lupus nephritis, and was initially described as being seronegative renal lupus. However, these two entities are now considered different pathological processes. Its association with rheumatoid arthritis is unusual, and there are no cases with a similar presentation reported in the literature. In this article, the case is presented of a man who developed both these conditions.

Resumen La nefropatía C1q, se describió por primera vez en 1985, como un proceso de glomerulonefritis con depósito mesangial de C1q, histológicamente similar a la nefritis lúpica, siendo inicialmente descrita como lupus renal seronegativo, sin embargo, estas dos entidades se consideran actualmente como procesos patológicos diferentes. Su asociación con artritis reumatoide es inusual y la literatura no reporta casos con presentación similar. A continuación, presentamos el caso de un hombre que desarrolla estas dos entidades.
Descritores: Artrite Reumatoide
Nefropatias
-Processos Patológicos
Associação
Células Mesangiais
Glomerulonefrite
Histologia
Limites: Humanos
Masculino
Adulto
Responsável: CO356.9


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Texto completo SciELO Brasil
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Id: biblio-983684
Autor: Ren, Wei; Zhao, Chen; Wang, Yan; Fang, Yuan; Huang, Zhenzhen; Chen, Wei; Wang, Lihua; Hu, Wen; Wang, Ke; Ni, Lijun.
Título: Ramipril can alleviate the accumulation of renal mesangial matrix in rats with diabetic nephropathy by inhibiting insulin-like growth factor-1
Fonte: Acta cir. bras;34(1):e20190010000007, 2019. tab, graf.
Idioma: en.
Projeto: Annual Science and Technology Projects of Anhui Province.
Resumo: Abstract Purpose: To investigate the impact of Ramipril (RAM) on the expressions of insulin-like growth factor-1 (IGF-1) and renal mesangial matrix (RMM) in rats with diabetic nephropathy (DN). Methods: The Sprague Dawley rats were divided into normal control (NC) group (n = 12), DN group (n = 11), and DN+RAM group (n = 12). The ratio of renal weight to body weight (RBT), fasting blood glucose (FBG), HbA1c, 24-h urine protein (TPU), blood urea nitrogen (BUN), creatinine (Cr), renal pathological changes, the levels of IGF-1, fibronectin (FN), type IV collagen (Col-IV), and matrix metalloproteinases (MMP)-2 were compared among the groups. Results: Compared with NC group, the RBT, FBG, HbA1c, TPU, BUN, Cr, and RMM in DN group were significantly increased (P < 0.05), the IGF-1, FN, and Col-IV were significantly upregulated (P < 0.05), while MMP was significantly downregulated (P < 0.05). Compared with DN group, the indexes except for the FBG and HbA1c in DN+RAM group were significantly improved (P < 0.05), among which IGF-1 exhibited significant positive correlation with TPU(r=0.937), FN(r=0.896) and Col-IV(r=0.871), while significant negative correlation with MMP-2 (r=-0.826) (P<0.05). Conclusion: RAM may protect the kidneys by suppressing IGF-1 and mitigating the accumulation of RMM.
Descritores: Fator de Crescimento Insulin-Like I/antagonistas & inibidores
Inibidores da Enzima Conversora de Angiotensina/farmacologia
Ramipril/farmacologia
Nefropatias Diabéticas/tratamento farmacológico
Células Mesangiais/efeitos dos fármacos
-Fator de Crescimento Insulin-Like I/metabolismo
Imuno-Histoquímica
Fibronectinas/efeitos dos fármacos
Fibronectinas/metabolismo
Ratos Sprague-Dawley
Metaloproteinases da Matriz/efeitos dos fármacos
Metaloproteinases da Matriz/metabolismo
Colágeno Tipo IV/efeitos adversos
Colágeno Tipo IV/metabolismo
Nefropatias Diabéticas/metabolismo
Células Mesangiais/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-742574
Autor: Berlin, Alejandro; Fernández, Mario I.
Título: Avances en el tratamiento de cáncer de próstata resistente a la castración: énfasis en nuevas terapias hormonales / Advances in the treatment of castration-resistant prostate cancer: emphasis in new hormonal therapies
Fonte: Rev. méd. Chile;143(2):223-236, feb. 2015. ilus, tab.
Idioma: es.
Resumo: Prostate cancer represents the second cancer-related cause of death in North American and Chilean men. The main treatment for incurable stages of disease is surgical or pharmacological castration. However, with time and despite the addition of anti-androgens, the disease progresses to a clinical state that has been commonly referred to as “hormone refractory”. In recent years, the concept of hormone refractoriness has been challenged and replaced by “castration resistance”, acknowledging that further and optimal hormonal manipulation can be attained, beyond achieving testosterone levels at castration range. The purpose of this review is to summarize the recent therapeutic breakthroughs in the management of metastatic castrate resistant prostate cancer (mCRPC), with greater emphasis in the newer hormonal therapy agents such as Abiraterone and Enzalutamide. Future combination and sequential treatment strategies are contextualized in the current era of personalized cancer medicine and genomic characterization of prostate cancer.
Descritores: Angiotensina II/fisiologia
Fibronectinas/biossíntese
Células Mesangiais/metabolismo
Inibidor 1 de Ativador de Plasminogênio/biossíntese
Poli(ADP-Ribose) Polimerases/fisiologia
-Células Cultivadas
Fibronectinas/genética
Regulação Enzimológica da Expressão Gênica
Mesângio Glomerular/citologia
Mesângio Glomerular/metabolismo
Mesângio Glomerular/patologia
Glomerulonefrite/genética
Glomerulonefrite/metabolismo
Glomerulonefrite/patologia
Células Mesangiais/enzimologia
Células Mesangiais/patologia
Inibidor 1 de Ativador de Plasminogênio/genética
Poli(ADP-Ribose) Polimerases/biossíntese
Poli(ADP-Ribose) Polimerases/genética
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/farmacologia
Limites: Animais
Ratos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-716939
Autor: Rodríguez Ospino, Hilmer Eduardo; González Paganti, Luciana; Lobo, Jorge.
Título: Nefropatía mesangial primaria por IgA e IgM / Primary IgA and IgM mesangial nephropathy
Fonte: Rev. nefrol. diál. traspl;33(2):75-84, jun. 2013. tab, graf.
Idioma: es.
Resumo: Introducción: La Nefropatía Mesangial Primaria(NMP) es un conjunto heterogéneo de patologías caracterizada por expansión de la matriz extracelular, depósitos mesangiales, frecuentemente de IgA o IgM, acompañados o no de hipercelularidad. Nuestros objetivos fueron establecer la prevalencia de NMP en el Hospital Militar Central, determinar características demográficas, epidemiológicas y evolución. Métodos: Estudio retrospectivo observacional. Se revisaron historias clínicas e informes de biopsias renales. Se obtuvieron datos de laboratorio, se registraron fechas de consultas, inicio de síntomas, remisión de la proteinuria y tratamiento realizado. Resultados: De 106 biopsias, 24 (20.2%) fueron NMP, 20 IgA y 4 IgM. Presentaron alteración aislada del sedimento urinario (52.2%), síndrome nefrótico(26.1 %) y síndrome nefrítico (21.7 %). Veintiún pacientes recibieron bloqueantes del sistema renina- angiotensina, 12 inmunosupresión. La proteinuria inicial fue 2.93 (±2.10) g/d, al año: 0.95 ±2.1 gr/d (p: 0.06), a los 2 años 0.55 ±0.7 gr/d (p: 0.03). Obtuvieron remisión completa (50%), completa-recaída (25%), parcial (20%), sin remisión(5%). Tiempo para la remisión 355.39 días (IC: 101-610). Pacientes con proteinuria menor a 1gr/d: remisión completa (29%), remisión-recaída (57%), parcial (14%). Con proteinuria mayor a 1gr/d: remisión completa (64%), remisión-recaída (9%), parcial (18%), sin remisión (9%). Tiempo de seguimiento 79.92 ± 87.9 meses. Ocho pacientes fueron seguidos 5 años, creatinina inicial 1.13± 0.4, final 1.58 ±1.1 mg/ dl (p=0.67). Proteinuria inicial 2.93±2.8 gr/d y final de 0.77±0.88 gr/d, (p=0.069). Un paciente requirió diálisis transitoria, otro diálisis crónica y falleció. Conclusiones: La NMP constituye la primera patología renal biopsiada. La proteinuria disminuyó significativamente. Los pacientes con proteinuria mayor a 1gr tuvieron mayor remisión y menor recaída, la creatinina mostró una tendencia ascendente no significativa.

Introduction: Primary Mesangial Nephropathy(PMN) is an heterogeneous set of pathologies characterized by the expansion of the extracellular matrix, mesangial deposits -frequently of IgA or IgM-, associated or not to hypercellularity. Our objectives were to establish the prevalence of PMN at the Hospital Militar Central, determine demographic and epidemiologic characteristics, and evolution. Methods: Observational retrospective study. Medical records and kidney biopsy reports have been reviewed. Laboratory data were obtained, consultation dates were recorded, as well as onset of symptoms, remission of proteinuria, and therapy. Results: Out of 106 biopsies, 24 (20.2%) were PMN, 20 IgA, and 4 IgM. They presented isolated abnormalities of the urine sediment (52.2%), nephrotic syndrome (26.1 %), and nephritic syndrome (21.7%). Twenty-one patients received renin-angiotensin system receptor blockers, 12 received immunosuppression. Baseline proteinuria was 2.93 (±2.10) gr/d, one year later: 0.95 ±2.1 gr/d (p: 0.06), two years later: 0.55 ±0.7 gr/d (p: 0.03). They had full remission (50%), full relapse (25%), partial remission (20%), and no remission (5%). Time until remission: 355.39 days (CI: 101-610). Patients with proteinuria lower than 1gr/d: full remission (29%), remission-relapse (57%), partial remission(14%). With proteinuria higher than 1gr/d: full remission (64%), remission-relapse (9%), partial remission (18%), no remission (9%). Follow-up time: 79.92 ± 87.9 months. Eight patients were followed up for 5 years, baseline creatinine 1.13± 0.4, final 1.58 ±1.1 mg/dL (p=0.67). Baseline urinary protein of 2.93±2.8 gr/d, and final value of 0.77±0.88 gr/d, (p=0.069). One patient required transient dialysis, another one required chronic dialysis and died. Conclusions: PMN is the first biopsied renal pathology. Urinary protein decreased considerably. Patients with urinary protein over 1gr had higher remission and lower relapse; creatinine showed an upward tendency that was not significant.
Descritores: Glomerulonefrite por IGA
Imunoglobulina M
Células Mesangiais
-Nefropatias
Limites: Humanos
Responsável: AR444.1 - BAN - Biblioteca Argentina de Nefrología Dr. Víctor R. Miatello


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Id: lil-716938
Autor: Hernández, Germán T; Baradaran, Azar; Nasri, Hamid.
Título: Significado de la ubicación de los depósitos de igA (mesangio capilares versus mesangiales puros) en la nefropatía igA y su asociación con las variables morfológicas de la clasificación de Oxford y varios datos demográficos / Significance of igA deposits location (mesangiocapillary versus pure mesangial) in igA nephropathy and its association with morphologic variables of Oxford classification and various demographic data
Fonte: Rev. nefrol. diál. traspl;33(2):68-74, jun. 2013. tab.
Idioma: es.
Resumo: Introducción: La nefropatía IgA se caracteriza por la presencia de depósitos glomerulares con predominio de IgA. Dentro de la descripción de la nefropatía IgA, existe una variación en la ubicación de los depósitos de inmunoglobulina A, desde el área mesangial hasta las paredes capilares. Objetivo: El objetivo de este estudio es determinar la posible correlación entre la ubicación de los depósitos de IgA y las variables morfológicas de la clasificación de Oxford (MEST, por sus siglas en inglés). Proliferación (hipercelularidad) mesangial (M) y endocapilar (E), glomeruloesclerosis(S) y atrofia tubular y fibrosis intersticial (T), y diversos datos clínicos de pacientes con nefropatía por inmunoglobulina. Métodos: El diagnóstico patológico de la nefropatía IgA requiere la demostración de depósitos inmunes con predominio de IgA con un patrón mesangial o mesangiocapilar a través de la microscopia por inmunofluorescencia (IF por su sigla en ingles). Los depósitos inmunes fueron semicuantificados con grados de fluorescencia de 0 a 3 cruces (+). La definición de la nefropatía por IgA requiere la presencia de depósitos de IgA difusos y globales con ≥ 2 + de fluorescencia y la ausencia de depósitos de C I q. Todas las biopsias renales realizadas entre julio del 2009 y julio de 2012, fueron enviadas a nuestro laboratorio de patología renal para analizarlas. Ninguno de los pacientes fue tratado antes de habérsele realizado la biopsia. Las biopsias con menos de 8 glomérulos fueron excluidas del estudio. Ninguno de los pacientes recibió un diagnóstico de nefropatía IgA, si había antecedentes de enfermedad vascular del colágeno o cirrosis hepática en los cuestionarios clínicos, los análisis de laboratorio o en el historial médico obtenidos al ingresar a los pacientes para realizarles la biopsia renal. Resultados: Un total de 114 biopsias fueron incluidas en el estudio. La edad media de los pacientes fue de 37,7 ± 13,6 años. Los pacientes se dividieron en dos grupos: depósitos puros mesangiales y depósitos mesangiocapilares. El número medio de glomérulos obtenidos por biopsia fue de 14,8 ± 7,2. El nivel medio de la proteinuria fue de 1742 ± 1324 mg /día (mediana = 1500 mg /día). En todas las biopsias, el número mediode glomérulos totalmente esclerosados fue de 2,4 ± 2,9 (mediana =1 glomérulo). Asimismo, la media del nivel de creatinina sérica fue de 1,6 ± 1,5 /dl (mediana = 1,2 mg / dl). En este estudio, el 10,5 por ciento de las biopsias renales tenían depósito de IgA mesangiocapilares. No se encontró ninguna asociación significativa entre la proporción de glomérulos totalmente esclerosados, la proliferación extracapilar, el porcentaje de fibrosis peri-glomerular, el engrosamiento de la cápsula de Bowman, el porcentaje de fibrosis intersticial, la proliferación mesangial de cualquier grado, o el ensanchamiento mesangial con depósitos mesangiales puros o depósitos mesangiocapilares (p≤ 0,05). No hubo ninguna asociación significativa entre la edad, la creatinina sérica y los niveles de proteinuria con depósitos mesangiales puros o mesangiocapilares (p≥0,05). Entre las cuatro variables morfológicas de la clasificación MEST de Oxford, únicamente la variable E (proliferación endocapilar) tuvo asociación significativa con depósitos mesangiocapilares (p=0,04) Conclusiones: La asociación entre depósitos mesangiocapilares IgA y la proliferación endocapilar puede implicar una mayor gravedad de la enfermedad por nefropatía IgA. Por lo tanto, se recomienda que la ubicación y la intensidad de los depósitos de IgA se incluyan de forma sistemática en los informes de biopsia renal.

Introduction: IgA nephropathy is characterized by the presence of IgA-dominant glomerular deposits. Within this description, there is variation in the location of this immunoglobulin, from mesangial area to capillary walls. Objectives: The aim of this study is to determine the potential correlation between the location of IgA deposits and morphologic variables of Oxford classification (MEST) and various clinical data of patients with immunoglobulin A nephropathy (IgAN). Results: A total of 114 biopsies were enrolled to the study. Mean age of patients was 37.7 ± 13.6 years. Patients were divided into two groups of pure mesagnial and mesangiocapillary deposits. In this study 10.5 percent of renal biopsies had mesangial-capillary IgA deposits. There was not significant association of proportion of totally sclerosed glomeruli, extracapillary, proliferation, percentage of peri-glomerular fibrosis, thickening of the Bowman's capsule, perent of interstitial fibrosis, mesangial proliferation in any degree and mesangial widening with pure mesangial or mesangial- capillary deposits (p>0.05).There was not significant association of age, serum creatinine and levels of proteinuria with pure mesangial or mesangiocapillary deposits (p>0.05).Among four morphologic variables of Oxford classification only E variable (endocapillary proliferation) had significant association with mesangiocapillary deposits (P=0.04). Conclusion: The association of mesangiocapillary IgA deposits with endocapillary proliferation may imply the severity of the disease. We recommend that the location and intensity of IgA is routinely included in the renal biopsy report.
Descritores: Glomerulonefrite por IGA
Células Mesangiais
-Biópsia
Glomerulonefrite Membranoproliferativa
Limites: Humanos
Responsável: AR444.1 - BAN - Biblioteca Argentina de Nefrología Dr. Víctor R. Miatello


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Texto completo SciELO Brasil
Boim, Mirian Aparecida
Texto completo
Id: lil-697085
Autor: Razvickas, Clara Versolato; Borges, Fernanda Teixeira; Oliveira, Andréia Silva de; Schor, Nestor; Boim, Mirian Aparecida.
Título: The effect of hypoxia and reoxygenation in the response of mesangial cells to angiotensin II in vitro / Efeito da hipóxia e reoxigenação na resposta à angiotensina II em células mesangiais in vitro
Fonte: J. bras. nefrol;35(4):259-264, out.-dez. 2013. ilus, tab.
Idioma: en.
Resumo: INTRODUCTION: Mesangial cells (MC) may be involved in the glomerular alterations induced by ischemia/reperfusion injury. OBJECTIVE: To evaluate the response of immortalized MC (IMC) to 30 minutes of hypoxia followed by reoxygenation periods of 30 minutes (H/R30) or 24 hours (H/R24). METHODS: The intracellular calcium concentration ([Ca+2]i) was measured before (baseline) and after adding angiotensin II (AII, 10-5 M) in the presence and absence of glybenclamide (K ATP channel blocker). We estimated the level of intracellular ATP, nitric oxide (NO) and PGE2. RESULTS: ATP concentration decreased after hypoxia and increased after reoxygenation. Hypoxia and H/R induced increases in basal [Ca+2]i. AII induced increases in [Ca+2]i in normoxia (97 ± 9%), hypoxia (72 ± 10%) or HR30 (85 ± 17%) groups, but there was a decrease in the response to AII in group H/R24 since the elevation in [Ca+2]i was significantly lower than in control (61 ± 10%, p < 0.05). Glybenclamide did not modify this response. It was observed a significant increase in NO generation after 24 hours of reoxygenation, but no difference in PGE2 production was observed. Data suggest that H/R injury is characterized by increased basal [Ca+2]i and by an impairment in the response of cells to AII. Results suggest that the relative insensibility to AII may be at least in part mediated by NO but not by prostaglandins or vasodilator K ATP channels. CONCLUSION: H/R caused dysfunction in IMC characterized by increases in basal [Ca+2]i during hypoxia and reduction in the functional response to AII during reoxygenation.

INTRODUÇÃO: Células mesangiais (CM) podem estar envolvidas na lesão glomerular induzida por hipoxia/reperfusão (H/R). OBJETIVO: Avaliar a resposta de CM imortalizadas (CMI) à hipoxia por 30 minutos seguida de reoxigenação por 30 minutos (H/R30) ou 24 horas (H/R24). MÉTODOS: Concentração de cálcio intracelular ([Ca+2]i) foi avaliada antes (basal) e após a adição de angiotensina II (AII, 10-5 M), na presença e na ausência de glibenclamida (bloqueador de canais K ATP). Foram estimados o nível de ATP intracelular, de óxido nítrico (NO) e de PGE2. RESULTADOS: Nível de ATP diminuiu após hipóxia e aumentou após a reoxigenação. H/R induziu aumento na [Ca+2]i basal. A AII elevou a [Ca+2]i nas condições de normoxia (97 ± 9%), hipoxia (72 ± 10%) ou HR30 (85 ± 17%), porém no grupo H/R24, houve diminuição significativa na resposta à AII, uma vez que a elevação da [Ca+2]i foi mais baixa do que no controle (61 ± 10%, p < 0,05). Glibenclamida não alterou esta resposta. Houve um aumento significativo na geração de NO após 24 horas de reoxigenação, mas não foi observada nenhuma diferença na produção de PGE2. Os dados indicam que a injuria celular causada pela hipoxia/reoxigenação é caracterizada pelo aumento na [Ca+2]i basal e por uma diminuição na reatividade celular à AII. Resultados sugerem que a insensibilidade ao agonista constritor pode ser pelo menos em parte, mediada pelo NO, mas não pelas prostaglandinas ou por canais K ATP. CONCLUSÃO: H/R resultou em disfunção das CMI, caracterizada pelo aumento na [Ca+2]i basal durante a hipóxia e redução da resposta funcional a AII durante a reoxigenação.
Descritores: Angiotensina II/farmacologia
Células Mesangiais/efeitos dos fármacos
Células Mesangiais/fisiologia
-Angiotensina II/fisiologia
Hipóxia Celular
Células Cultivadas
Cálcio/metabolismo
Oxigênio/farmacologia
Fatores de Tempo
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-697082
Autor: Younes-Ibrahim, Maurício.
Título: Células mesangiais: protagonistas ou coadjuvantes da função renal? / Mesangial cells: renal function protagonists or coadjuvants?
Fonte: J. bras. nefrol;35(4):248-249, out.-dez. 2013. ilus.
Idioma: pt.
Descritores: Rim/fisiologia
Células Mesangiais/fisiologia
Limites: Humanos
Tipo de Publ: Editorial
Responsável: BR1.1 - BIREME



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