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Id: biblio-960121
Autor: Galván, Gonzalo; Guerrero-Martelo, Manuel; Vásquez De la Hoz, Francisco.
Título: Cannabis: A cognitive illusion / Cannabis: una ilusión cognitiva
Fonte: Rev. colomb. psiquiatr;46(2):95-102, Apr.-June 2017. tab.
Idioma: en.
Resumo: Abstract Introduction: The vision of cannabis as a soft drug is due to the low risk perception that young and old people have of the drug. This perception is based on erroneous beliefs that people have about the drug. Objective: To compare the beliefs of cannabis use and consequences among adolescents with a lifetime prevalence of cannabis use and those without a lifetime prevalence of cannabis use. Method: Quantitative, descriptive and cross-sectional study with a probability sample of 156 high school students who completed an ad-hoc questionnaire that included sociodemographic data and 22 questions about the beliefs that young people had about cannabis use and its consequences. Results : The lifetime prevalence of cannabis use was 13.5%. The prevalence group consisted mostly of males. Statistically significant differences between different groups and different beliefs were found. The group with no lifetime prevalence of cannabis use perceived higher risk as regards the damage that cannabis can cause to memory, other cognitive functions, neurons, mental health, and general health. The group with a lifetime prevalence of cannabis use perceived a lower risk as regards the use of cannabis, and think that intelligent people smoke cannabis, and that cannabis has positive effects on the brain, increasing creativity and is used to cure mental diseases. Conclusions: Those who used cannabis once in their life perceive the use of the substance as less harmful or less potential danger to health compared to those who never consumed. In fact those who consumed at some time even have beliefs that suggest positive effects in those people that consume it.

Resumen Introducción: La visión del cannabis como una droga blanda responde a una baja percepción de riesgo que jóvenes y adultos tienen de la droga; esta percepción se funda en creencias erróneas acerca de la droga. Objetivo: Comparar las creencias acerca del cannabis, su uso y sus consecuencias en adolescentes con prevalencia de vida de uso de cannabis y aquellos sin prevalencia de vida de uso de cannabis. Método: Estudio cuantitativo, descriptivo y transversal que evaluó una muestra probabilística de 156 estudiantes de enseñanza secundaria con un instrumento ad-hoc que incluyó datos sociodemográficos y 22 preguntas acerca de las creencias que tienen los jóvenes sobre cannabis, su uso y sus consecuencias. Resultados: Se hallaron diferencias estadísticamente significativas entre los diferentes grupos y las diferentes creencias. El grupo sin prevalencia de vida de uso de cannabis percibe mayor riesgo del daño que puede ocasionar el cannabis en la memoria, otras funciones cognitivas, las neuronas, la salud mental y la salud general. El grupo de prevalencia de vida de uso de cannabis percibe menos riesgo del uso de cannabis y piensa que la gente inteligente fuma cannabis, que el cannabis tiene efectos positivos para el cerebro, que aumenta la creatividad y que se utiliza para curar enfermedades mentales. Conclusiones: Quienes consumieron cannabis alguna vez en la vida perciben el uso de la sustancia como menos nocivo o con menor potencial de peligrosidad para la salud que quienes nunca consumieron. De hecho quienes consumieron alguna vez incluso tienen creencias que sugieren efectos positivos en los seres humanos que consumen.
Descritores: Cannabis
Saúde Mental
-Estudantes/psicologia
Estudos Transversais
Cognição
Cérebro
Uso da Maconha
Neurônios
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Responsável: CO78 - Asociación Colombiana de Psiquiatría


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Id: biblio-1088663
Autor: Pino Rodríguez, Esteban; Kunizawa Larrosa, Héctor Tatsuya; Yamuy, Jack; Borde Bebeacua, Michel.
Título: Modelo in vitro para el estudio del papel de la unión mesopontina en la generación del sueño de movimientos oculares rápidos y la vigilia / In vitro model for the study of the role of the mesopontine region in rapid eye movement (REM) sleep and wakefulness / Modelo in vitro para o estudo do papel da união mesopontina na geração de sono de movimentos oculares rápidos e da vigília
Fonte: An. Facultad Med. (Univ. Repúb. Urug., En línea);4(1):103-136, jul. 2017. ilus.
Idioma: es.
Resumo: El estudio de las estrategias neurales para la organización del comportamiento en vertebrados constituye un desafío mayor para la Neurociencia. El avance del conocimiento en este campo depende de manera crítica de la utilización de modelos experimentales adecuados que admitan múltiples niveles de análisis (p.ej: comportamental, circuital, celular, sináptico, molecular) y abordajes multitécnicos. Nos propusimos analizar in vitro una red neural de la unión mesopontina del tronco encefálico críticamente implicada en el control del sueño de movimientos oculares rápidos (S-REM). Pese al cúmulo de evidencias que apoyan el papel desempeñado por esta red en relación al S-REM, los mecanismos celulares y sinápticos que subyacen a este control son poco conocidos y continúan siendo objeto de intensa investigación. Para avanzar en el conocimiento de estos mecanismos, se llevó a cabo la caracterización morfológica y funcional de una rodaja de tronco encefálico de la rata, en la que las estructuras críticas para el control del S-REM, i.e.: núcleos tegmentales laterodorsal y pedúnculopontino, y su proyección al núcleo reticular pontis oralis (PnO), están presentes y son operativas. La inclusión del núcleo motor del trigémino en la rodaja permitió detectar cambios de la excitabilidad de las motoneuronas ante manipulaciones farmacológicas del PnO, representativos de los cambios del tono muscular asociados a maniobras similares realizadas in vivo. La utilización de este modelo in vitro de S-REM, permitirá aportar a la dilucidación de las estrategias neurales que operan en niveles intermedios de organización del SN en mamíferos para la generación y regulación de un estado comportamental.

The study of the neural basis of behavior is a major challenge in Neuroscience. Advancing our knowledge in this field depends, critically, on the use of experimental paradigms that provide multiple levels of analysis, as well as powerful techniques. We have selected, as a model of a neural plan that organizes a complex behavior, a neural network located in the mesopontine junction. This region is thought to be both necessary and sufficient for the generation of rapid eye movement (REM) sleep, although the cellular and synaptic mechanisms involved in the control of this behavioral state at the mesopontine level are still under debate and remain poorly understood. As part of a long term effort to gain insight into these mechanisms, we carried out the morphological and functional characterization of a slice preparation of rat brainstem and we demonstrate that critical structures for the control of REM sleep - the laterodorsal and pedunculopontine tegmental nuclei and their projection to the oral part of the pontine reticular nucleus (PnO) - are present and are operational. The presence of the trigeminal motor nucleus in the slice sought to include in the experimental model a structure capable of expressing changes of the excitability of the motorneurons caused by pharmacological manipulations of the PnO, representative of changes of muscle tone associated with similar maneuvers performed in vivo. The use of this in vitro model of REM sleep will provide critical information to elucidate neural strategies that operate at intermediate levels of central nervous system organization in mammals to control behavioral states.

O estudo de estratégias neurais para a organização do comportamento em vertebrados constitui um desafio maior para a Neurociencia. O avanço do conhecimento nessa área depende criticamente da utilização de modelos experimentais adequados que suportem múltiplos níveis de análise (por exemplo: comportamental, circuital, celular, sináptico e molecular) e abordagens por múltiplas técnicas. Decidiu-se analisar in vitro uma rede neural da união mesopontina do tronco encefálico criticamente envolvida no controle do sono de movimentos oculares rápidos (S-REM). Apesar da riqueza de provas que sustentam o papel desta rede em relação ao S-REM, os mecanismos celulares e sinápticos subjacentes a este controle são pouco conhecidos e permanecem sob intensa investigação. Para avançar no conhecimento desses mecanismos, caracterizou-se morfológica e funcionalmente uma fatia de tronco encefálico de rato, na qual as estruturas críticas para o controle do S-REM, i.e.: núcleos tegmentais laterodorsal e pedunculopontino, e sua projeção para o núcleo reticular pontis oralis (PnO) estão presentes e operantes. A inclusão do núcleo motor do trigêmeo na fatia permitiu detectar mudanças da excitabilidade das motoneuronas provocadas por manipulações farmacológicas do PnO, representativas das alterações do tônus muscular associados com operações semelhantes quando realizados in vivo. A utlização deste modelo in vitro de S-REM permitirá contribuir para a elucidação de estratégias neurais que operam em níveis intermedios de organização do SN de mamíferos para a geração e regulação de um estado comportamental.
Descritores: Sono REM/fisiologia
Vigília/fisiologia
Polissonografia
Neurônios/fisiologia
-Técnicas In Vitro
Tronco Encefálico/anatomia & histologia
Ratos Wistar
Estimulação Elétrica
Fenômenos Eletrofisiológicos
Limites: Animais
Ratos
Tipo de Publ: Estudo Observacional Veterinário
Responsável: UY1.1 - BINAME - Biblioteca Nacional de Medicina


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Id: biblio-1254059
Autor: Roldán, Guillermo; Gómez Tabares, Gustavo.
Título: Síntomas vasomotores en la menopausia: una mirada a la fisiología / Vasomotor symptoms in menopause: a look to physiology
Fonte: Rev. colomb. menopaus;26(2):7-15, 2020.
Idioma: es.
Resumo: En este artículo colocamos en contexto la edad y el envejecimiento, incluimos la menopausia como desarrollo en la edad media de la mujer y examinamos detenidamente la fisiología del eje gonadal a la luz de los últimos descubrimientos del inicio de la menstruación, su desarrollo hasta la etapa de la transición menopáusica y la explicación actual del origen de los síntomas vasomotores. También se revisan los tratamientos derivados de los nuevos conocimientos acerca de la regulación de gonadotropinas que podrían ser utiles como alternativa en las mujeres que no toleran o que tienen contraindicación absoluta de la terapia hormonal convencional con estrógeno y progestágenos.

In this article we put age and aging into context, include menopause as a development in the middle age of women, and carefully examine the physiology of the gonadal axis in light of the latest discoveries of the onset of menstruation, its development to the stage of the menopausal transition and the current explanation of the origin of vasomotor symptoms. The treatments derived from the new knowledge about the regulation of gonadotropins that could be useful as an alternative in women who do not tolerate or have absolute contraindication to conventional hormonal therapy with estrogen and progestogens are also reviewed.
Descritores: Menopausa
-Sinais e Sintomas
Estrogênios
Neurônios
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Revisão
Responsável: CO5.1 - Centro de Información y Conocimiento


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Miglino, Maria Angélica
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Id: biblio-1134463
Autor: Barreto, Rodrigo S. N; de-Oliveira, Franceliusa Delys; Matias, Gustavo de Sá Schiavo; Rodrigues, Marcio N; Carvalho, Rafael C; Franciolli, André Luis R; Fratini, Paula; Miglino, Maria Angelica.
Título: Rabbit vomeronasal organ-derivered cells have mesenchymal profile and neuronal commitment / Las células derivadas del órgano vomeronasal del conejo tienen perfil mesenquimatoso y compromiso neuronal
Fonte: Int. j. morphol;38(5):1463-1472, oct. 2020. graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium.

RESUMEN: El órgano vomeronasal (OVN) es un órgano accesorio de la vía olfatoria, que detecta feromonas y emite señales que afectan la modulación del comportamiento social y reproductivo. Las células madre OVN reemplazan las neuronas durante toda la vida. El objetivo de este estudio fue aislar y caracterizar células derivadas del órgano vomeronasal de conejos raza Nueva Zelanda. Para el aislamiento y el cultivo celular se utilizaron cinco conejos machos con una edad de 120 días. Las células del OVN presentaron etiquetado para la proliferación (PCNA), un perfil indiferenciado (Nanog), neuronal (GFAP), células madre mesenquimales (CD73, CD90 y CD105 y Stro-1). Además, se ob- servó presencia de citoesqueleto (Vimentina, β-tubulina y CK-18) y ausencia de marcadores hematopoyéticos (CD34, CD117 y CD45) tanto por inmunofluorescencia como por citometría de flujo. Me- diante PCR fue posible verificar la expresión de algunos genes de perfil indiferenciado (Oct-4), neuronal (Nestin) y mesenquimatoso (CD73, CD105 y Vimentin). Las células derivadas del OVN se diferencian in vitro en adipocitos, osteocitos y condrocitos, y no presentan un potencial tumorigénico al ser infiltrados en ratones Balb / c nu / nu. En conclusión, las células derivadas de OVN de conejo tienen un perfil que podría ser compatible con el epitelio olfatorio / neurorreceptor de OVN transmitiendo factores al recambio epitelial o incluso mediante la diferenciación en células epiteliales para reemplazar el epitelio comisural.
Descritores: Coelhos/anatomia & histologia
Órgão Vomeronasal/citologia
Células-Tronco Mesenquimais/fisiologia
-Bulbo Olfatório/citologia
Células-Tronco/fisiologia
Mucosa Olfatória/citologia
Reação em Cadeia da Polimerase
Imunofluorescência
Citometria de Fluxo
Neurônios/fisiologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132180
Autor: Ribeiro, Iára Mariana Léllis; Pereira, Wagner Luiz; Nogueira, Leonardo Brandão; Oliveira, Laser Antônio Machado; Teixeira, Róbson Ricardo; Nogueira, Katiane de Oliveira Pinto Coelho.
Título: Neuroprotective Effect of Isobenzofuranones on Hydrogen Peroxide-Mediated Redox Imbalance in Primary Cultures of Hippocampal Neurons
Fonte: Braz. arch. biol. technol;63:e20190072, 2020. graf.
Idioma: en.
Projeto: Minas Gerais Funding Foundation.
Resumo: Abstract In live organisms, there is a balance between the production of reactive oxygen species (ROS) and their neutralization. The increased level of these species leads to a condition called redox imbalance. The aim of this study was to evaluate the protective action of isobenzofuranones in primary cultures of hippocampal neurons subjected to redox imbalance. To accomplish this, MTT and LIVE/DEAD assays were initially performed. In the cultures pretreated with isobenzofuranones 1 and 2, there was a higher number of live cells when compared to that in the untreated ones. Regarding redox imbalance, there was a significant increase in the intracellular levels of ROS. The cultures pretreated with isobenzofuranones showed a reduction in ROS levels. Lipid peroxidation caused by oxidative damage was significantly reduced in the cultures pretreated with isobenzofuranones 1 and 2. Taken together, these data show the ability of isobenzofuranones 1 and 2 to significantly minimize cytotoxicity, cell death, intracellular levels of ROS and lipid peroxidation induced by redox imbalance. These results suggest that isobenzofuranones 1 and 2 represent a possible alternative therapy for the neurodegenerative disturbances that are triggered by ROS production increases.
Descritores: Oxirredução/efeitos dos fármacos
Benzofuranos/farmacologia
Espécies Reativas de Oxigênio
Fármacos Neuroprotetores/farmacologia
Peróxido de Hidrogênio
-Benzofuranos/síntese química
Morte Celular
Cultura Primária de Células
Hipocampo/citologia
Neurônios/metabolismo
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1115470
Autor: Guardo-Gómez, Verónica; Fajardo-Perdomo, María Alexandra; Muñoz, Ana L; Gómez, Lina A.
Título: Ingeniería Tisular Para Regeneración Nerviosa: Una Revisión / Tissue Engineering For Nervous Regeneration: A Review
Fonte: Rev. chil. neuro-psiquiatr;58(1):50-60, mar. 2020.
Idioma: es.
Resumo: Resumen Introducción: Este artículo presenta avances de la medicina regenerativa y la ingeniería de tejidos orientados a la regeneración de neuronas, de axones y nervios. Revisamos las técnicas que existen actualmente, las más utilizas o prometedoras, en la búsqueda de avances para regenerar este tipo de tejidos. Objetivo: Con esta revisión queremos describir el conocimiento actual sobre la medicina regenerativa y la ingeniería de tejidos orientados a la reparación de tejidos nerviosos. Metodología: Para desarrollar esta revisión se realizó una búsqueda de artículos entre los años 2007 y el 2018, la búsqueda se restringió a los artículos que incluyeran dentro de sus palabras clave; Ingeniería tisular, Enfermedades Neurodegenerativas, Medicina regenerativa, Regeneración axonal, Regeneración neuronal, Regeneración tisular. Con el fin de seleccionar los artículos más adecuados, se realizó una búsqueda exhaustiva en bases de datos como Springer, Medline Ebsco y Science direct. Conclusiones: Se mencionan técnicas como implantación de injertos, terapia celular y terapia molecular e implantación de andamios 3D para regeneración de neuronas, axones y nervios; a partir de esta revisión pudimos observar que estas técnicas en su mayoría funcionan mejor cuando se combinan, aprovechando las ventajas de cada una para promover la regeneración de los diferentes tejidos nerviosos.

Introduction: This article presents advances in regenerative medicine aimed at the regeneration of nervous and neuronal tissue, focusing on regeneration of neurons, axons and nerve regeneration. We will review the techniques that currently exist, the most used or promising, in the search of advances to regenerate this type of tissues. Objective: With this review we want to describe the current knowledge about regenerative medicine and tissue engineering oriented to nerve tissue repair. Methodology: To carry out this review, a search of articles was carried out between 2007 and 2018, the search was restricted to the articles that they included within their keywords; Tissue Engineering, Neurodegenerative Diseases, Regenerative Medicine, Axonal Regeneration, Neuronal Regeneration, Tissue Regeneration. We will mention about techniques such as implantation. Conclusions: with this review we could observe that most of the mentioned techniques work better when combined, taking advantage of each one to promote a greater regeneration of the different tissues.
Descritores: Axônios
Doenças Neurodegenerativas
Engenharia Tecidual
Terapia Baseada em Transplante de Células e Tecidos
Tecido Nervoso
Neurônios
Tipo de Publ: Revisão
Responsável: CL58.1 - Biblioteca


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Id: biblio-950819
Autor: Koriauli, S; Natsvlishvili, N; Barbakadze, T; Mikeladze, D.
Título: Knockdown of interleukin-10 induces the redistribution of sigma1-receptor and increases the glutamate-dependent NADPH-oxidase activity in mouse brain neurons
Fonte: Biol. Res;48:1-5, 2015. graf.
Idioma: en.
Resumo: BACKGROUND: In the central nervous system, interleukin-10 (IL-10) provides trophic and survival effects directly on neurons, modulates neurite plasticity, and has a pivotal importance in the neuronal regeneration in neurodegenerative and neuroinflammatory conditions. This cytokine is primarily produced by glial cells and has beneficial effects on the neuronal viability. However, the mechanisms of IL-10-elicited neuroprotection are not clear. RESULTS: Membrane preparations, isolated from wild-type (Wt) and IL-10 knockout (KO) mice brain were used in this study. It has been shown that compared to wild-type mice, in IL-10 KO mice brain, the amount of immunoglobulin binding protein (BiP) is greatly increased, whereas the content of sigma receptor-1 (SigR1) is not changed significantly. Co-immunoprecipitation experiments have shown that the association of SigR1 with small GTPase Rac1 (Ras-related C3 botulinum toxin substrate 1), NR2B subunit of NMDA-receptor (NMDAR) and inositol-3-phosphate receptor (IP3R) is higher in the IL-10 KO mice brain than in the Wt mice brain. Besides, we have found that either glutamate or sigma ligands, separately or together, do not change glutamate-induced NADPH-oxidase (NOX) activity in Wt-type mice brain membrane preparations, whereas in IL-10 KO mice high concentration of glutamate markedly increases the NOX-dependent production of reactive oxygen species (ROS). Glutamate-dependent ROS production was decreased to the normal levels by the action of sigma-agonists. CONCLUSIONS: It has been concluded that IL-10 deprivation, at least in part, can lead to the induction of ER-stress, which causes BiP expression and SigR1 redistribution between components of endoplasmic reticulum (ER) and plasma membrane. Moreover, IL-10 deficiency can change the specific organization of NMDAR, increasing the surface expression of SigR1-sensitive NR2B-containing NMDAR. In these conditions, glutamate-dependent ROS production is greatly increased leading to the initiation of apoptosis. In this circumstances, sigma-ligands could play a preventive role against NMDA receptor-mediated excitotoxicity.
Descritores: Encéfalo/metabolismo
Interleucina-10/genética
Receptores sigma/metabolismo
Ácido Glutâmico/metabolismo
NADPH Oxidases/metabolismo
-Membrana Celular/metabolismo
Receptores sigma/classificação
Receptores sigma/agonistas
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Receptores de N-Metil-D-Aspartato/classificação
Receptores de N-Metil-D-Aspartato/metabolismo
Proteínas rac1 de Ligação ao GTP/metabolismo
Imunoprecipitação
Retículo Endoplasmático/metabolismo
Receptores de Inositol 1,4,5-Trifosfato/metabolismo
Técnicas de Silenciamento de Genes
Proteínas de Choque Térmico/metabolismo
Camundongos Endogâmicos C57BL
Neurônios/metabolismo
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-792512
Autor: Sica, Roberto E; Caccuri, Roberto; Quarracino, Cecilia; Capani, Francisco.
Título: Are astrocytes executive cells within the central nervous system? / ¿Son los astrocitos células ejecutivas dentro del Sistema Nervioso Central?
Fonte: Arq. neuropsiquiatr;74(8):671-678, Aug. 2016.
Idioma: en.
Resumo: ABSTRACT Experimental evidence suggests that astrocytes play a crucial role in the physiology of the central nervous system (CNS) by modulating synaptic activity and plasticity. Based on what is currently known we postulate that astrocytes are fundamental, along with neurons, for the information processing that takes place within the CNS. On the other hand, experimental findings and human observations signal that some of the primary degenerative diseases of the CNS, like frontotemporal dementia, Parkinson’s disease, Alzheimer’s dementia, Huntington’s dementia, primary cerebellar ataxias and amyotrophic lateral sclerosis, all of which affect the human species exclusively, may be due to astroglial dysfunction. This hypothesis is supported by observations that demonstrated that the killing of neurons by non-neural cells plays a major role in the pathogenesis of those diseases, at both their onset and their progression. Furthermore, recent findings suggest that astrocytes might be involved in the pathogenesis of some psychiatric disorders as well.

RESUMEN Evidencias experimentales sugieren que los astrocitos desempeñan un rol crucial en la fisiología del sistema nervioso central (SNC) modulando la actividad y plasticidad sináptica. En base a lo actualmente conocido creemos que los astrocitos participan, en pie de igualdad con las neuronas, en los procesos de información del SNC. Además, observaciones experimentales y humanas encontraron que algunas de las enfermedades degenerativas primarias del SNC: la demencia fronto-temporal; las enfermedades de Parkinson, de Alzheimer, y de Huntington, las ataxias cerebelosas primarias y la esclerosis lateral amiotrófica, que afectan solo a los humanos, pueden deberse a astroglíopatía. Esta hipótesis se sustenta en hallazgos que demostraron que la muerte neuronal que en ellas ocurre es debida al compromiso de células no-neuronales que juegan rol principal en su iniciación y desarrollo. Más aún, observaciones recientes señalan que los astrocitos podrían estar implicados en la patogenia de algunas enfermedades psiquiátricas.
Descritores: Astrócitos/fisiologia
Doenças Neurodegenerativas/fisiopatologia
Demência/fisiopatologia
Neurônios/fisiologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-973951
Autor: Kooshki, Razieh; Abbasnejad, Mehdi; Esmaeili-Mahani, Saeed; Raoof, Maryam.
Título: The effect of CA1 administration of orexin-A on hippocampal expression of COX-2 and BDNF in a rat model of orofacial pain / O efeito da administração de CA1 de orexina-A na expressão hipocampal de COX-2 e BDNF em um modelo de dor orofacial em ratos
Fonte: Arq. neuropsiquiatr;76(9):603-608, Sept. 2018. graf.
Idioma: en.
Resumo: ABSTRACT The neuropeptide orexin-A and its receptors are widely distributed in both hippocampal circuitry and pain transmission pathways. Objective: Involvement of the CA1 orexin 1 receptor (OX1R) on the modulation of orofacial pain and pain-induced changes in hippocampal expression of cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) was investigated. Methods: Orofacial pain was induced by an intra-lip injection of capsaicin (100 μg). Reverse transcription polymerase chain reaction and immunoblot analysis were used to indicate changes in hippocampal BDNF and COX-2 expression, respectively. Results: Capsaicin induces a significant pain response, which is not affected by either orexin-A or SB-334867-A, an OX1R antagonist. However, an increased expression of COX-2 and decreased expression of BDNF was observed in the hippocampus of animals that received capsaicin or SB-334867-A (80 nM) plus capsaicin. Meanwhile, orexin-A (40 pM) attenuated the effects of capsaicin on the expression of COX-2 and BDNF. Conclusions: CA1 OX1R activation moderates capsaicin-induced neuronal inflammation and neurotrophic deficiency.

RESUMO O neuropeptídeo orexina-A e seus receptores estão amplamente distribuídos nos circuitos do hipocampo e nas vias de transmissão da dor. Objetivo: O envolvimento do receptor de orexina 1 CA1 (OX1R) na modulação da dor orofacial e alterações induzidas pela dor na expressão do hipocampo de ciclooxigenase-2 (COX-2) e fator neurotrófico derivado do cérebro (BDNF) foi investigado. Métodos: A dor orofacial foi induzida por injeção intra-labial de capsaicina (100 μg). A reação em cadeia da polimerase de transcrição reversa e a análise de imunotransferência foram utilizadas para indicar alterações na expressão de BDNF e COX-2 no hipocampo, respectivamente. Resultados: A capsaicina induz uma resposta significativa à dor, que não é afetada pela orexina-A ou pelo SB-334867-A, um antagonista do OX1R. No entanto, uma expressão aumentada de COX-2 e uma expressão diminuída de BDNF foi observada no hipocampo de animais que receberam capsaicina ou SB-334867-A (80 nM) mais capsaicina. Enquanto isso, a orexina A (40 pM) atenuou os efeitos da capsaicina na expressão de COX-2 e BDNF. Conclusões: A ativação de CA1 OX1R modera a inflamação neuronal induzida por capsaicina e a deficiência neurotrófica.
Descritores: Dor Facial/metabolismo
Fator Neurotrófico Derivado do Encéfalo/metabolismo
Ciclo-Oxigenase 2/metabolismo
Receptores de Orexina/metabolismo
Orexinas/farmacologia
Hipocampo/metabolismo
-Ureia/análogos & derivados
Ureia/farmacologia
Benzoxazóis/farmacologia
Capsaicina
Ratos Wistar
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Modelos Animais de Doenças
Hipocampo/efeitos dos fármacos
Naftiridinas
Neurônios/efeitos dos fármacos
Neurônios/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-950836
Autor: Codocedo, Juan Francisco; Inestrosa, Nibaldo C.
Título: Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
Fonte: Biol. Res;49:1-10, 2016. ilus, graf.
Idioma: en.
Projeto: Basal Center for Excellence in Science and Technology; . Comisión Nacional de Investigación Científica y Tecnológica.
Resumo: BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs (miRNAs) are a family of small non-coding RNAs that control the gene expression of their targets through hybridization with complementary sequences in the 3' UTR, thereby inhibiting the translation of the target proteins. Several evidences indicate that the miRNAs are actively involved in the regulation of neuronal function. RESULTS: In the present study, we examined whether Wnt-5a modulates the levels of miRNAs in hippocampal neurons. Using PCR arrays, we identified a set of miRNAs that respond to Wnt-5a treatment. One of the most affected miRNAs was miR-101b, which targets cyclooxygenase-2 (COX2), an inducible enzyme that converts arachidonic acid to prostanoids, and has been involved in the injury/inflammatory response, and more recently in neuronal plasticity. Consistent with the Wnt-5a regulation of miR-101b, this Wnt ligand regulates COX2 expression in a time-dependent manner in cultured hippocampal neurons. CONCLUSION: The biological processes induced by Wnt-5a in hippocampal neurons, involve the regulation of several miRNAs including miR-101b, which has the capacity to regulate several targets, including COX-2 in the central nervous system
Descritores: MicroRNAs/fisiologia
Ciclo-Oxigenase 2/análise
Proteínas Wnt/fisiologia
Hipocampo/enzimologia
Neurônios/enzimologia
-Regulação para Baixo
Expressão Gênica
Células Cultivadas
Western Blotting
Ratos Sprague-Dawley
Marcação de Genes
Perfilação da Expressão Gênica
Reação em Cadeia da Polimerase em Tempo Real
Proteína Wnt-5a
Hipocampo/química
Plasticidade Neuronal
Neurônios/química
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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