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Id: biblio-899708
Autor: Martínez, Jahnnyer; Hernández, Juan C; Urcuqui-Inchima, Silvio.
Título: Papel de las células dendríticas en la infección por el virus dengue: blancos de replicación y respuesta inmune / Role of dendritic cells in infection by dengue virus: targets for replication and immune response
Fonte: Rev. chil. infectol;34(3):249-256, jun. 2017. ilus, tab.
Idioma: es.
Projeto: Colciencias; . Universidad de Antioquia.
Resumo: Dengue fever, caused by dengue virus (DENV) infection, is one of the most important diseases in the world, not only due to the high morbidity/mortality rates it causes, but also because of its great economic and social impact in tropical/subtropical countries. DENV infection has a wide range of clinical manifestations ranging from asymptomatic infection or infection with mild symptoms to severe dengue that can lead to death. At present, no etiological treatment or effective globally distributed vaccine against the four DENV serotypes exists. Despite great efforts made to understand the mechanism associated with DENV disease pathogenesis the causes leading to severe dengue presentation have not been clarified. Some hypotheses seek to give a biological and physiological explanation to the clinical manifestations that appear during the infection. Based on the evidence that after contact with dendritic cells DENV alters the functionality of these cells, this review aims to describe the most relevant findings regarding the importance of dendritic cells in the context of DENV infection and progression of the illness.

El dengue, causada por el virus dengue (DENV), es una de las enfermedades más importantes no sólo por los altos índices de morbilidad/mortalidad, sino también por su gran impacto económico y social en los países de las regiones tropicales/subtropicales. La infección por el DENV cursa por un variado rango de manifestaciones clínicas que van desde una infección asintomática o con síntomas leves, hasta el dengue grave que puede ser fatal. En la actualidad, no se dispone de un tratamiento etiológico y tampoco de una vacuna eficaz mundialmente distribuida, contra los 4 serotipos del DENV. A pesar de los grandes esfuerzos orientados a entender el mecanismo asociado con la patogénesis de la enfermedad, aún no se ha logrado esclarecer de forma definitiva las causas que conllevan a las formas graves de enfermedad. Algunas hipótesis buscan dar una explicación biológica y fisiológica a las manifestaciones clínicas que se presentan durante la infección. Dado que una de ellas sugiere que luego del contacto con las células dendríticas el DENV altera su funcionalidad, la presente revisión tiene como objetivo describir los hallazgos más relevantes referentes a la importancia de dichas células en el marco de la infección por el DENV y progresión de la enfermedad.
Descritores: Replicação Viral/imunologia
Células Dendríticas/imunologia
Dengue/imunologia
Vírus da Dengue/imunologia
-Progressão da Doença
Imunidade Celular
Imunidade Inata
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1045757
Autor: Hou, G; Lu, J; Wang, H; Liu, Q; Tian, G; Yang, Y.
Título: Chemokine receptor 7 implications of spleen dendritic cells in multiple-organ dysfunction syndrome in mice / Implicaciones del receptor de la quimiocina de tipo 7 de las células dendríticas del bazo en el síndrome de disfunción orgánica múltiple en ratones
Fonte: West Indian med. j;62(9):787-792, Dec. 2013. ilus, graf.
Idioma: en.
Projeto: \"Eleventh Five\" Medical Research Project of PLA.
Resumo: OBJECTIVE: This study aims to explore the chemokine receptor 7 (CCR7) expression of spleen dendritic cells (DCs) and their role in the changes of migration and activity of spleen DCs in multiple-organ dysfunction syndrome (MODS). METHODS: The MODS model of mice was reproduced. The mice were randomly assigned to the following groups: normal, three-hour to six-hour, 24-hour to 48-hour, and 10-day to 12-day postzymosan injection. CD11c and CD205 were analysed by immunohistochemistry; the expressions of CD86 and CCR7 of DCs were studied using flow cytometry analyses. RESULTS: In normal mice, many DCs were found at the margin between the red and white pulp. In the three-hour to six-hour and 24- to 48-hour group, DC effectively upregulated CD86 and CCR7, and they were distributed in T-cell areas. In the 10-day to 12-day group, DCs were distributed at the margin by the immature form. CONCLUSION: The CCR7 expression level of DCs had close correlations with the migration of DCs. Chemokine receptor 7 can be used to evaluate the migration and functional activity of DCs in MODS.

OBJETIVO: Este estudio persigue explorar la expresión del receptor de la quimiocina 7 (CCR7) de células dendríticas del bazo (CD), y su papel en los cambios de la migración y la actividad del las células DC del bazo en el síndrome de disfunción orgánica múltiple (SDOM). MÉTODOS: Se reprodujo el modelo SDOM de los ratones. Los ratones fueron asignados aleatoriamente a los siguientes grupos de inyección de post-zymosan: hora normal, tres a seis horas, 24 horas a 48 horas, y de 10 a 12 días. CD11c y CD205 fueron analizados mediante inmunohistoquímica. Las expresiones de CD86 y CCR7 de CD se estudiaron mediante análisis de citometría de flujo. RESULTADOS: En los ratones normales, muchas células CD fueron encontradas en el margen entre la pulpa roja y la blanca. En el grupo de tres a seis horas y el grupo de 24 a 48 horas, CD86y CCR7 fueron efectivamente sobre-regulados en CD, y distribuidos en las áreas de células T. En el grupo de 10 a 12 días, las CDs fueron distribuidas en el margen por la forma inmadura. CONCLUSIÓN: El nivel de expresión CCR7 de las CDs tuvo estrecha correlación con la migración de las CDs. El receptor de la quimiocina de tipo 7 puede utilizarse para evaluar la migración y la actividad funcional de las CDs en SDOM.
Descritores: Baço/citologia
Células Dendríticas/imunologia
Receptores de Quimiocinas/imunologia
Insuficiência de Múltiplos Órgãos/patologia
-Imuno-Histoquímica
Movimento Celular
Modelos Animais de Doenças
Camundongos Endogâmicos C57BL
Insuficiência de Múltiplos Órgãos/imunologia
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-892856
Autor: Hwang, Eu Chang; Jung, Seung Il; Lee, Hyun-Ju; Lee, Je-Jung; Kwon, Dong Deuk.
Título: Generation of potent cytotoxic T lymphocytes against in male patients with non-muscle invasive bladder cancer by dendritic cells loaded with dying T24 bladder cancer cells
Fonte: Int. braz. j. urol;43(4):615-627, July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background In order to induce a potent cytotoxic T lymphocyte (CTL) response in dendritic cell (DC)-based immunotherapy for bladder cancer, various tumor antigens can be loaded onto DCs. Objective The aim of this study was to establish a method of immunotherapy for male patients with non-muscle invasive bladder cancer (NMIBC), using bladder cancer-specific CTLs generated in vitro by DCs. Materials and Methods Monocyte-derived DCs from bladder cancer patients were induced to mature in a standard cytokine cocktail (IL-1β, TNF-α, IL-6, and PGE2: standard DCs, sDCs) or anα-type 1-polarized DC (αDC1) cocktail (IL-1β, TNF-α, IFN-α, IFN-γ, and polyinosinic:polycytidylic acid) and loaded with the UVB-irradiated bladder cancer cell line, T24. Antigen-loaded αDC1s were evaluated by morphological and functional assays, and the bladder cancer-specific CTL response was analyzed by cytotoxic assay. Results The αDC1s significantly increased the expression of several molecules pertaining to DC maturation, regardless of whether or not the αDC1s were loaded with tumor antigens, relative to sDCs. The αDC1s demonstrated increased production of interleukin-12 both during maturation and after subsequent stimulation with CD40L that was not significantly affected by loading with tumor antigens as compared to that of sDCs. Bladder cancer-specific CTLs targeting autologous bladder cancer cells were successfully induced by αDC1s loaded with dying T24 cells. Conclusion Autologous αDC1s loaded with an allogeneic bladder cancer cell line resulted in increased bladder cancer-specific CTL responses as compared to that with sDCs, and therefore, may provide a novel source of DC-based vaccines that canbe used in immunotherapy for male patients with NMIBC.
Descritores: Neoplasias da Bexiga Urinária/terapia
Células Dendríticas/imunologia
Linfócitos T Citotóxicos/imunologia
Citocinas/uso terapêutico
Imunoterapia/métodos
-Neoplasias da Bexiga Urinária/imunologia
Diferenciação Celular/imunologia
Resultado do Tratamento
Linhagem Celular Tumoral
Imunoterapia/efeitos adversos
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Idoso
Responsável: BR1.1 - BIREME


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Id: lil-792649
Autor: Murad, Henrique; Murad, Felipe Francescutti.
Título: The role of age in the abdominal aortic aneurysm repair
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);31(2):III-III, Mar.-Apr. 2016.
Idioma: en.
Descritores: Aneurisma da Aorta Abdominal
Procedimentos Endovasculares
Molécula A de Adesão Juncional
-Células Dendríticas
Linfócitos T
Limites: Humanos
Tipo de Publ: Comentário
Editorial
Responsável: BR1.1 - BIREME


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Id: biblio-950776
Autor: Thao, Nguyen Phuong; Luyen, Bui Thi Thuy; Koo, Jung Eun; Kim, Sohyun; Koh, Young Sang; Cuong, Nguyen Xuan; Nam, Nguyen Hoai; Van Kiem, Phan; Kim, Young Ho; Van Minh, Chau.
Título: Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Projeto: Vietnam Academy of Science and Technology; . National Research Foundation of Korea; . Ministry of Education, Science, and Technology.
Resumo: BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S)5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.
Descritores: Estrelas-do-Mar/química
Células Dendríticas/efeitos dos fármacos
Interleucina-6/farmacologia
Fator de Necrose Tumoral alfa/farmacologia
Subunidade p40 da Interleucina-12/farmacologia
Anti-Inflamatórios/análise
-Esteroides/administração & dosagem
Vietnã
Ensaio de Imunoadsorção Enzimática
Sobrevivência Celular/efeitos dos fármacos
Lipopolissacarídeos
Interleucina-6/análise
Fator de Necrose Tumoral alfa/análise
Concentração Inibidora 50
Subunidade p40 da Interleucina-12/análise
Cultura Primária de Células
Camundongos Endogâmicos C57BL
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950823
Autor: Chen, Haide; Li, Yang; Lin, Xijuan; Cui, Di; Cui, Chun; Li, Hui; Xiao, Lei.
Título: Functional disruption of human leukocyte antigen II in human embryonic stem cell
Fonte: Biol. Res;48:1-9, 2015. ilus, graf.
Idioma: en.
Projeto: Ministry of Agriculture; . National Natural Science Foundation of China; . Zhejiang Provincial Natural Science Foundation of China; . Agricultural Variety Breeding Project of Zhejiang Province; . Ministry of Science and Technology of China.
Resumo: BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification. RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA(-/-)hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from CIITA(-/-)hESCs expressed CD83 and CD86 but without the constitutive HLA II. Fibroblasts derived from CIITA(-/-)hESCs were powerless in IFN-γ inducible expression of HLA II. CONCLUSION: We generated HLA II defected hESCs via deleting CIITA, a master regulator of constitutive and IFN-γ inducible expression of HLA II genes. CIITA(-/-)hESCs can differentiate into tissue cells with non-HLA II expression. It's promising that CIITA(-/-)hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors' CD4+ T cells, which are the main effector cells of cellular immunity in allograft.
Descritores: Proteínas Nucleares/genética
Transativadores/genética
Diferenciação Celular/genética
Deleção de Genes
Desoxirribonucleases/metabolismo
Células-Tronco Embrionárias Humanas/metabolismo
-Teratoma
Células Dendríticas/metabolismo
Imunoglobulinas/metabolismo
Imuno-Histoquímica
Glicoproteínas de Membrana/metabolismo
Células Tumorais Cultivadas
Antígenos de Histocompatibilidade Classe II/genética
Antígenos CD/metabolismo
Interferon gama/metabolismo
Camundongos SCID
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Desoxirribonucleases/classificação
Antígeno B7-2/metabolismo
Corpos Embrioides/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Cariótipo
Fibroblastos/metabolismo
Autorrenovação Celular
Células Apresentadoras de Antígenos/metabolismo
Limites: Humanos
Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132267
Autor: Alawiyah, Kamila; Juliandi, Berry; Boediono, Arief; Sasai, Noriaki.
Título: Oral Administration of Incense Resin (Styrax benzoin) Extract Enhances Spatial Learning, Memory, and Dendrite Complexity of Mice
Fonte: Braz. arch. biol. technol;63:e20180379, 2020. tab, graf.
Idioma: en.
Projeto: Ministry of Research, Technology, and Higher Education, Indonesia to Berry Juliandi; . Global Collaboration Program; . Japan Society of Promotion of Science.
Resumo: Abstract Hippocampus is a part of the brain that has a major role in spatial learning and memory which can be affected by herbal extracts. Incense resin (Styrax benzoin) has been used by local communities to improve intelligence. However, there is no scientific evidence of the functions of Styrax benzoin for regulating hippocampal function. The aim of this study was intended to analyze and investigate the effect of incense resin on learning, memory, and dendrite complexity of mice. Three months old male Deutch Democratic Yokohama (DDY) mice were injected orally with graded doses of 100, 150, and 200 mg/kg of incense resin aqueous extract daily for 30 days. Spatial learning and memory performance levels were tested with Y-maze alternation, novel object recognition, and Morris water maze. The branches and maximum dendritic span in the dentate gyrus were observed by the Golgi-Cox staining. Overall, our results showed that incense resin extract increased learning and memory ability, and the number of dendrite branching in the dentate gyrus.
Descritores: Células Dendríticas/efeitos dos fármacos
Extratos Vegetais/farmacologia
Styrax/química
Aprendizagem Espacial/efeitos dos fármacos
Memória/efeitos dos fármacos
-Administração Oral
Aprendizagem em Labirinto/efeitos dos fármacos
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-900873
Autor: Encalada-García, Carlos.
Título: Células dendríticas e interferones en el lupus eritematoso sistémico / Dendritic cells and interferons in systemic lupus erythematosus
Fonte: Rev. colomb. reumatol;24(3):177-184, jul.-set. 2017. tab, graf.
Idioma: es.
Resumo: Resumen El lupus eritematoso sistémico (LES) es un trastorno autoinmune con base genética, caracterizado por la aparición de autoanticuerpos, formación y depósito de complejos inmunes circulantes e inflamación crónica en varios órganos. La etiología es multifactorial y, en individuos genéticamente predispuestos, factores medioambientales y componentes hormonales juegan un rol clave en el sistema inmune de esta enfermedad. Cerca de 25 loci genéticos han sido identificados, indicando la importancia en esta enfermedad; sin embargo, la tasa de concordancia para el LES es de tan solo el 25% entre gemelos monocigotos (1,2). Un ejemplo de ello son las deficiencias de los componentes iniciales en la vía clásica del complemento sérico como el C1q, C2 o C4, que si bien es infrecuente, confieren susceptibilidad genética para el LES en una tasa del 30% en caso de deficiencia del C4 y de más del 90% para una deficiencia del C1q (3). Por otro lado, se demostró que el C1q inhibe a las células dendríticas plasmocitoides (CDP) en la secreción de interferón alfa (IFN-a), proporcionando así un nuevo enlace entre la deficiencia del complemento y la activación de la vía del IFN (4). Por ello, el IFN-a es considerado como un actor central en la patogénesis del LES, encontrándose concentraciones séricas altas en los brotes de esta enfermedad (5). En consecuencia, estos IFN ejercen efectos claves en la fisiopatología del LES, lo que sugiere que esta citoquina no solo posee un efecto a nivel del sistema inmune innato, sino también en las respuestas inmunes adaptativas. Teniendo en cuenta estos hechos, se puede anticipar que las CDP, fuente principal de secreción de IFN, están involucradas en dicha enfermedad autoinmune. En esta revisión nos centraremos en la participación de las CDP y del IFN en el LES (6,7).

Abstract Systemic Lupus Erythematosus (SLE) is an autoimmune disorder with a genetic basis, and is characterised by the appearance of autoantibodies, the formation and deposition of circulating immune complexes, and chronic inflammation in various organs. It is of multifactorial origin, and in genetically predisposed individuals, environmental factors and hormonal components play a key role in the immune system of the disease. About 25 genetic loci have been identified, indicating the importance in this pathology. However, the concordance rate for SLE is only 25% among monozygotic twins. An example of this is the deficiencies of the initial components in the classical serum complement pathway such as C1q, C2 or C4, which, although rare, confer genetic susceptibility for SLE at a rate of 30% in the case of C4 deficiency, and more than 90% for C1q deficiency. It was also demonstrated that C1q inhibits plasmacytoid dendritic cells (pDCs) in the secretion of interferon-alpha (IFN-a), thus providing a new link between complement deficiency and activation of the IFN pathway. Therefore, IFN-a is considered to have a central role in the pathogenesis of SLE, with high serum concentrations being found in outbreaks of this disease. These IFN exert prominent immunoregulatory effects, suggesting that this cytokine is key, not only in the innate immune system, but also in adaptive immune responses. Taking these facts into account, it can be anticipated that pDCs, the main source of IFN secretion, are involved in this autoimmune disease. In this review, we will focus on the participation of pDCs and IFNs in SLE.
Descritores: Células Dendríticas
Interferons
Lúpus Eritematoso Sistêmico
-Autoanticorpos
Inflamação
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CO356.9


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Id: lil-790772
Autor: Núñez, César; Lozada-Requena, Iván; Ysmodes, Tíndara; Zegarra, Daniel; Saldaña, Fatima; Aguilar, José.
Título: Inmunomodulación de Uncaria tomentosa sobre células dendríticas, IL-12 y perfil TH1/TH2/TH17 en cáncer de mama / Immunomodulation of Uncaria tomentosa over dendritic cells, IL-12 and profile TH1/TH2/TH17 in breast cancer
Fonte: Rev. peru. med. exp. salud publica;32(4):643-651, oct.-dic. 2015. graf.
Idioma: es.
Resumo: Evaluar el efecto inmunomodulador del extracto estandarizado (5,03%, alcaloides oxindólicos pentacíclicos) de Uncaria tomentosa (UT-POA) sobre el inmunofenotipo de células dendríticas (DC) y IL-12/Th1/Th2/Th17 en pacientes con cáncer de mama estadio II (BCII) y mujeres sanas (H). Materiales y métodos. Se obtuvó sangre de 11 H y 7 BCII, se aislaron y cultivaron PBMC por 2 h con/sin distintas concentraciones de UT-POA y se estimularon o no con LPS por 24 h. Las PBMC fueron marcadas con anticuerpos específicos para DC y en el sobrenadante se midió las citoquinas Th1/Th2/Th17, ambos por citometría de flujo. Además, se midió IL-12 por ELISA. Resultados. UT-POA no alteró a las DC o la expresión de moléculas accesorias en BCII. Sin embargo, en H mostró una disminución en el porcentaje de DC mieloides (mDC) con incremento de HLA-DR y CD86 a 1000 ug/mL. La secreción de IL-12 fue modificada solo en H, incrementando la subunidad p70 y disminuyendo la p40. UT-POA incremento Th1 (IFN-y y IL-2), Th2 (IL-4) y Th17 (IL-17) en BCII y H. Conclusiones. UT-POA incrementa la producción de citoquinas relacionadas con la respuesta antitumoral a concentraciones entre el rango de 500-1000 ug/mL. Este efecto positivo debería ser evaluado no solo sistemicamente sino también en el microambiente tumoral. La UT-POA puede ser un fitoquímico útil en la quimioprevención y en el uso alternativo con terapias contra el cáncer...

This study aimed to research the in vitro immunomodulatory effects of an Uncaria tomentosa hydroalcoholic extract standardized (5.03%, pentacyclic oxindole alkaloids) (UT-POA) on the immunophenotype of dendritic cells (DC) subsets, Th1, Th2, Th17 and IL-12 cytokines from patients with stage II breast cancer (BCII) and healthy women (H). Materials and methods. Blood of 11 H and 7 BCII was obtained, PBMC were isolated and cultured for 2h with/without various concentrations of UT-POA and stimulated or not with LPS for 24h. PBMC were labeled with specific antibodies for DC and in the supernatant we measured Th1/Th2/Th17 cytokines, both by flow cytometry. Furthermore IL-12 was measured by ELISA. Results. UT-POA did not alter DC or accessory molecules expression in BCII. However, H exhibited a decrease in the percentage of mDC (myeloid DC) and an increase in HLA-DR and CD86 expression at 1000 ug/mL. IL-12 secretion was modified only in the H group, increasing p70 subunit and decreasing p40 subunit. UT-POA increased Th1 (IFN-y and IL-2), Th2 (IL-4) and Th17 (IL-17) secretion in both groups. Conclusions. UT-POA increased the production of cytokines related with anti-tumoral response at concentrations of 500-1000 ug/mL. This positive effect should be evaluated not only systemically but also in the tumor microenvironment in further studies. UT-POA may be a useful phytochemical in chemoprevention and in the alternative use in cancer therapies...
Descritores: Anti-Inflamatórios
Células Dendríticas
Neoplasias da Mama
Unha-de-Gato
Limites: Humanos
Adolescente
Adulto
Feminino
Criança
Adulto Jovem
Pessoa de Meia-Idade
Responsável: PE14.1 - Biblioteca de la Sede Central


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Id: lil-790771
Autor: Lozada-Requena, Iván; Núñez, César; Alvárez, Yubell; Kahn, Laura; Aguilar, José.
Título: Poblaciones linfocitarias, células dendríticas y perfil de citoquinas en ratones con melanoma tratados con Uncaria tomentosa / Lymphocyte subsets, dendritic cells and cytokine profiles in mice with melanoma treated with Uncaria tomentosa
Fonte: Rev. peru. med. exp. salud publica;32(4):633-642, oct.-dic. 2015. ilus, tab, graf.
Idioma: es.
Resumo: Evaluar el efecto inmunomodulador sobre poblaciones linfocitarias, células dendríticas (DC), citoquinas Th1/Th2/Th17 (T-helper) e inflamatorias en el ámbito sistémico y/o en el microambiente tumoral de ratones con o sin melanoma. Materiales y métodos. Se obtuvieron muestras de sangre periférica y/o de tumores primarios de ratones con melanoma B16 tratados o no con un extracto hidroalcohólico de Uncaria tomentosa (UT) con 5,03% de alcaloides oxindólicos pentacíclicos (UT-POA) obtenido de la corteza de la planta. Todos los ensayos de medición de células y citoquinas fueron realizados por citometría de flujo. Resultados. UT-POA a nivel sistémico incrementa la relación CD4/CD8a (Cluster of Differenciation), mientras que la activación celular es inversamente proporcional; incrementa la proporción de DCm (DC mieloides); induce un perfil Th1 proinflamatorio y reduce la respuesta Th17. TNF-a (tumor necrosis factor alpha) y IL-17A (interleuquina) correlacionan positiva y negativamente con la relación CD4/CD8a. Conclusiones. El incremento de Th1 (TNF-a) puede tener como consecuencia el incremento de linfocitos CD4 o la activación de macrófagos M1. Aunque UT-POA muestra un incremento de DCm, este no es dosis-dependiente. La disminución de Th17 (IL-17A) puede favorecer el funcionamiento de los linfocitos CD8a. UT-POA muestra mejores efectos inmunomoduladores en el ámbito sistémico que intratumoral...

To evaluate the immunomodulatory effect on lymphocyte subsets, dendritic cells (DC), Th1 / Th2 / Th17 and inflammatory cytokines on systemic level and/or in the tumor microenvironment of mice with or without melanoma. Materials and methods: Peripheral blood and/or primary tumors samples were obtained of mice with B16 melanoma treated or not with a hydroalcoholic extract of Uncaria tomentosa (UT) with 5.03% of pentacyclic oxindole alkaloids (UT-POA) obtained from the bark of the plant. All cell assays and cytokine measurements were performed by flow cytometry. Results. UT-POA systemically increased CD4/CD8a relation while cell activation was inversely proportional; increased the proportion of DCm; induced a pro-inflammatory Th1 profile and reduced Th17 response. TNF-a and IL-17A positively and negatively correlated with CD4/CD8a relation. Conclusions. The increase of Th1 (TNF-a) may result in the increase of CD4 or M1 macrophage activation. Although UT-POA shows increased DCm, is not dose-dependent. Th17(IL-17A) decreased can support the function of CD8a lymphocytes. UT-POA shows better systemic immunomodulatory effects than intratumoral...
Descritores: Ativação Linfocitária
Células Dendríticas
Unha-de-Gato
Limites: Animais
Camundongos
Responsável: PE14.1 - Biblioteca de la Sede Central



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