Base de dados : LILACS
Pesquisa : A11.251.210.172.500 [Categoria DeCS]
Referências encontradas : 10 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1

  1 / 10 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Id: biblio-1165166
Autor: Baquedano María Sonia; Guercio Gabriela; Marino Roxana; Berensztein Esperanza; Costanzo Mariana; Ramírez Pablo; Bailez Marcela; Vaiani Elisa; Maceiras Mercedes; Rivarola Marco A; Belgorosky Alicia.
Título: Nueva mutación heterocigota en el gen de la proteína regulatoria aguda de la esteroideogénesis (StAR) en un paciente 46,XY con hiperplasia adrenal congénita lipoidea / [Novel heterozygous mutation in the steroidogenic acute regulatory protein gene in a 46,XY patient with congenital lipoid adrenal hyperplasia].
Fonte: Medicina (B.Aires);73(4):297-302, jul.-ago. 2013.
Idioma: es.
Resumo: StAR facilitates cholesterol entry into the mitochondria as part of the transduceosome complex. Recessive mutations in the gen STAR cause classic and nonclassic congenital lipoid adrenal hyperplasia. The aim of the study was to analyze the molecular consequences of a novel heterozygous STAR mutation in a 46,XY patient with ambiguous genitalia and adrenal insufficiency. We found a de novo heterozygous IVS-2A>G STAR mutation and the reported heterozygous p.G146A SF1 polymorphism with normal CYP11A1, FDXR, FDX1, VDAC1 and TSPO genes. RT-PCR and sequencing from patient's testicular RNA showed a -exon2 transcript and the wild-type (WT) transcript. Both 37 kDa precursor and 30 kDa mature protein were detected in COS-7 cell transfected with mutant and WT plasmids. Immunofluorescence showed almost no co-localization of mitochondria and mutant protein (delta22-59StAR). Delta22-59StAR activity was 65±13

of WT. Cotransfection with WT and delta22-59StAR plasmids reduced WT activity by 62.0

± 13.9. Novel splice-junction heterozygous STAR mutation (IVS-2A>G) resulted in the in-frame loss of amino acids 22 to 59 in the N-terminal mitochondrial targeting signal. A misfolded p.G22_L59delStAR might interfere with WT StAR activity by blocking the transduceosome complex, causing an autosomal dominant form of StAR deficiency, explaining the clinical phenotype.
Descritores: Fosfoproteínas/genética
Hiperplasia Suprarrenal Congênita/genética
Mutação/genética
/genética
TRANSTORNO ABSTRACTING AND INDEXING,XY DO DESENVOLVIMENTO SEXUAL/genética
-Animais
Chlorocebus aethiops
Células COS
Fenótipo
Humanos
Insuficiência Adrenal/genética
Linhagem
Masculino
Polimorfismo Genético
Reação em Cadeia da Polimerase em Tempo Real
Recém-Nascido
Tipo de Publ: Relatos de Casos
Resumo em Inglês
Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


  2 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1017244
Autor: Xu, Xiaobo; Zhang, Ruiqing; Chen, Xinhua.
Título: Application of a single-chain fragment variable (scFv) antibody for the confirmatory diagnosis of hydatid disease in non-endemic areas
Fonte: Electron. j. biotechnol;29:57-62, sept. 2017. ilus, graf, tab.
Idioma: en.
Resumo: Background: Hydatid disease is a serious parasitic disease threatening public health. Because of its rarity in non-endemic coastal areas, determining the nature and origin of a chronic, enlarged liver cystic mass is challenging in these regions. Under these circumstances, physicians need a confirmatory diagnostic tool beyond immunological and radiological examinations. This study investigated a novel human single-chain fragment variable (scFv) antibody for the confirmative diagnosis of 18 atypical hydatid disease cases in non-endemic coastal areas. Results: A scFv antibody against cystic echinococcosis was produced by genetic engineering and then applied to the immunohistochemical diagnosis of 18 cases of cystic echinococcosis presented in non-endemic coastal areas. The diagnosis of these cases by ultrasound and serum-based examinations was inconclusive. The 750 bp scFv antibody gene was expressed in COS-7 cells, and the antibody localized in the cytoplasm. The scFv antibody can detect the germinal layer and protoscolices of actively growing cysts but not of the degenerating protoscolices and has a diagnostic efficiency higher than that of single serum or ultrasound testing (P b 0.05). The combined use of scFv antibodies with serology and ultrasound diagnostics results in a diagnostic efficiency comparable to that of surgery. The scFv antibody can be used as a confirmatory test for the diagnosis of hydatid disease in non-endemic areas, providing a beneficial supplementary diagnostic method that complements traditional immune testing and ultrasonic radiology and thus helping physicians to effectively differentiate hydatid disease.
Descritores: Equinococose/diagnóstico
Equinococose Hepática/diagnóstico
Anticorpos de Cadeia Única/química
-Imunoensaio
Testes Sorológicos
Imuno-Histoquímica
Células COS
Equinococose/diagnóstico por imagem
Equinococose Hepática/diagnóstico por imagem
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


  3 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-741526
Autor: Saffer, Fernanda; Lubianca, José Faibes Lubianca; Rösing, Cassiano; Dias, Caroline; Closs, Luciane.
Título: Predictors of Success in the Treatment of Obstructive Sleep Apnea Syndrome with Mandibular Repositioning Appliance: A Systematic Review
Fonte: Int. arch. otorhinolaryngol. (Impr.);19(1):80-85, Jan-Mar/2015. tab, graf.
Idioma: en.
Resumo: Introduction Obstructive sleep apnea syndrome affects up to 4% of middle-aged men and 2% of adult women. It is associated with obesity. Objective The objective of this article is to review the literature to determine which factors best correlate with treatment success in patients with obstructive sleep apnea syndrome treated with a mandibular repositioning appliance. Data Synthesis A search was performed of the PubMed, Cochrane, Lilacs, Scielo, and Web of Science databases of articles published from January 1988 to January 2012. Two review authors independently collected data and assessed trial quality. Sixty-nine articles were selected from PubMed and 1 from Cochrane library. Of these, 42 were excluded based on the title and abstract, and 27 were retrieved for complete reading. A total of 13 articles and 1 systematic review were considered eligible for further review and inclusion in this study: 6 studies evaluated anthropomorphic and physiologic factors, 3 articles addressed cephalometric and anatomic factors, and 4 studies evaluated variables related to mandibular repositioning appliance design and activation. All the studies evaluated had low to moderate methodologic quality and were not able to support evidence on prediction of treatment success. Conclusion Based on this systematic review on obstructive sleep apnea syndrome treatment, it remains unclear which predictive factors can be used with confidence to select patients suitable for treatment with a mandibular repositioning appliance. .
Descritores: Evolução Biológica
Proteínas de Transporte/química
Cinesina/química
Modelos Moleculares
Microtúbulos/metabolismo
-Transporte Biológico/fisiologia
Chlorocebus aethiops
Células COS
Dimerização
Transferência Ressonante de Energia de Fluorescência
Cinética
Microscopia de Fluorescência
Limites: Animais
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: BR1.1 - BIREME


  4 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-732873
Autor: Poloni, Priscila Ferreira; Omodei, Michelle Sako; Nahas-Neto, Jorge; Uemura, Gilberto; Véspoli, Heloisa De Luca; Nahas, Eliana Aguiar Petri.
Título: Prevalência da baixa densidade mineral óssea em mulheres na pós-menopausa tratadas de câncer de mama / Prevalence of low bone mineral density in postmenopausal breast cancer survivors
Fonte: Rev. bras. ginecol. obstet;37(1):30-35, 01/2015. tab.
Idioma: pt.
Projeto: Fundação de Amparo da Pesquisa do Estado de São Paulo.
Resumo: OBJETIVO: Avaliar a prevalência da baixa densidade mineral óssea (DMO) em mulheres na pós-menopausa tratadas de câncer de mama. MÉTODOS: Estudo de corte transversal que incluiu 115 mulheres tratadas de câncer de mama atendidas em Hospital Universitário do Sudeste do Brasil. Foram incluídas mulheres com amenorreia há 12 meses ou mais e 45 anos ou mais de idade, tratadas de câncer de mama e livres de doença há pelo menos 5 anos. A DMO foi mensurada pelos raios-X de dupla energia em coluna lombar (L1 a L4) e colo de fêmur. Considerou-se baixa DMO quando valores de T-score de coluna total e/ou colo de fêmur <-1,0 Score de Delphi (DP) (osteopenia e osteoporose). Por meio de entrevista, foram avaliados fatores de risco para baixa DMO. Na análise estatística, empregaram-se os testes do χ2 ou Exato de Fisher. RESULTADOS: A média de idade das pacientes foi 61,6±10,1 anos e o tempo de menopausa, 14,2±5,6 anos, com tempo médio de seguimento de 10,1±3,9 anos. Considerando coluna e colo de fêmur, 60% das mulheres tratadas de câncer de mama apresentavam baixa DMO. Avaliando os fatores de risco para baixa DMO, foi encontrada diferença significativa na distribuição percentual quanto à idade (maior porcentagem de mulheres com mais de 50 anos e baixa DMO), história pessoal de fratura prévia (11,6% com baixa DMO e nenhuma com DMO normal) e índice de massa corpórea. Maior frequência de obesidade foi observada entre mulheres com DMO normal (63%) quando comparadas àquelas com baixa DMO (26,1%; p<0,05). CONCLUSÃO: Mulheres na pós-menopausa tratadas de câncer de mama apresentaram elevada prevalência de baixa DMO (osteopenia e/ou osteoporose). .

PURPOSE: To evaluate the prevalence of low bone mineral density (BMD) in postmenopausal breast cancer survivors. METHODS: In this cross-sectional study, 115 breast cancer survivors, seeking healthcare at a University Hospital in Brazil, were evaluated. Eligibility criteria included women with amenorrhea ≥12 months and age ≥45 years, treated for breast cancer and metastasis-free for at least five years. BMD was measured by DEXA at the lumbar spine (L1-L4) and femoral neck. Low BMD was considered when total-spine and/or femoral-neck T-score values were <-1.0 Delphi Score (DP) (osteopenia and osteoporosis). The risk factors for low BMD were assessed by interview. Data were analyzed statistically by the χ2 test and Fisher's exact test. RESULTS: The mean age of breast cancer survivors was 61.6±10.1 years and time since menopause was 14.2±5.6 years, with a mean follow-up of 10.1±3.9 years. Considering spine and femoral neck, 60% of breast cancer survivors had low BMD. By evaluating the risk factors for low BMD, a significant difference was found in the percent distribution for age (higher % of women >50 years with low BMD), personal history of previous fracture (11.6% with low BMD versus 0% with normal BMD) and BMI. A higher frequency of obesity was observed among women with normal BMD (63%) compared to those with low BMD (26.1%) (p<0.05). CONCLUSION: Postmenopausal breast cancer survivors had a high prevalence of osteopenia and osteoporosis. .
Descritores: Ácidos e Sais Biliares/metabolismo
Canalículos Biliares/metabolismo
Proteínas de Transporte/metabolismo
Hidroxiesteroide Desidrogenases
Glicoproteínas de Membrana
-Adenosina Trifosfatases/metabolismo
Transporte Biológico
Células COS
Antígeno Carcinoembrionário/biossíntese
Proteínas de Transporte/biossíntese
Primers do DNA
DNA Complementar
Íleo/metabolismo
Cinética
Mutagênese Sítio-Dirigida
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/metabolismo
Transfecção
Ácido Taurocólico/metabolismo
Limites: Animais
Ratos
Tipo de Publ: Research Support, U.S. Gov't, P.H.S.
Responsável: BR1.1 - BIREME


  5 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-694713
Autor: Zou, Yi; Zhong, Wenping.
Título: A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton
Fonte: Biocell;36(3):127-132, Dec. 2012. ilus, graf.
Idioma: en.
Projeto: Science Foundation of the Ministry of Education of China; . Jinan University.
Resumo: PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity, indicating a potential role of PEPP2 in regulating function of membrane and microtubules.
Descritores: Membrana Celular/metabolismo
Citoesqueleto/metabolismo
Proteínas de Homeodomínio/metabolismo
-Androstadienos/farmacologia
Chlorocebus aethiops
Células COS
Difusão
Glutationa Transferase/metabolismo
Lipídeos/química
Microscopia de Fluorescência
Modelos Biológicos
Microtúbulos/metabolismo
Ligação Proteica
Estrutura Terciária de Proteína
Fosfatidilinositóis/química
Proteínas Recombinantes de Fusão/química
Transdução de Sinais
Viscosidade
Cicatrização
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  6 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-634475
Autor: Bidinost, C.; Saka, H.A.; Aliendro, O.; Sola, C.; Panzetta-Duttari, G.; Carranza, P.; Echenique, J.; Patrito, E.; Bocco, J.L..
Título: Virulence factors of non-O1 non-O139 Vibrio cholerae isolated in Córdoba, Argentina / Factores de virulencia de Vibrio cholerae no-O1 no-O139 aislados en Córdoba, Argentina
Fonte: Rev. argent. microbiol;36(4):158-163, Oct.-Dec. 2004. ilus, tab.
Idioma: en.
Resumo: V. cholerae non-O1 non-O139 serogroups isolated from clinical and environmental sources in Córdoba, Argentina, were analyzed for the presence and expression of virulence genes. Most of the strains studied contained the genes toxR and hlyA, but lacked ctxA, zot, ace, tcpA and stn. The culture supernatants were tested for hemolytic and cytotoxic activity. The enterotoxic potential of the strains was studied in a rabbit ileal loop assay and their genetic profiles were compared by PFGE. The environmental strains varied in their virulence phenotype and showed no-clonal relationships. The clinical strains were highly enterotoxic, hemolytic, proteolytic and showed indistinguishable PFGE profiles, although they differed in their cytotoxic activity. This is the first description, using cell culture and “in vivo” studies, of the virulence properties of non-O1 non-O139 V. cholerae from Argentina.

En este trabajo se analizó la presencia y expresión de genes de virulencia en V. cholerae no-O1 no-O139 de origen clínico y ambiental, aislados en Córdoba, Argentina. La mayoría de las cepas estudiadas contiene los genes toxR y hlyA, pero no ctxA, zot, ace, tcpA y stn. Se analizó la actividad hemolítica y citotóxica de estas cepas en los sobrenadantes de cultivo, así como su potencial enterotóxico en ensayos de asa ileal ligada de conejo. Además, los aislamientos fueron comparados por sus perfiles genéticos en PFGE. Las cepas del medio ambiente mostraron variación en su fenotipo de virulencia y no mostraron relación clonal. Las cepas clínicas fueron muy enterotóxicas, hemolíticas, proteolíticas y mostraron perfiles indistinguibles de PFGE, aunque mostraron diferencias en su actividad citotóxica. En este trabajo se describen por primera vez, utilizando ensayos de cultivo celular e “in vivo”, propiedades de virulencia de V. cholerae no-O1 no-O139 aislados en Argentina.
Descritores: Vibrio cholerae não O1/patogenicidade
-Argentina/epidemiologia
Técnicas de Tipagem Bacteriana
Proteínas de Bactérias/genética
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/fisiologia
Chlorocebus aethiops
Células COS/microbiologia
Toxina da Cólera/genética
DNA Bacteriano/genética
Farmacorresistência Bacteriana
Diarreia/epidemiologia
Diarreia/microbiologia
Eletroforese em Gel de Campo Pulsado
Enterotoxinas/genética
Enterotoxinas/isolamento & purificação
Enterotoxinas/fisiologia
Deleção de Genes
Genes Bacterianos
Proteínas Hemolisinas/genética
Proteínas Hemolisinas/isolamento & purificação
Proteínas Hemolisinas/fisiologia
Metaloendopeptidases/genética
Metaloendopeptidases/isolamento & purificação
Metaloendopeptidases/fisiologia
Filogenia
Vibrioses/epidemiologia
Vibrioses/microbiologia
Vibrio cholerae não O1/efeitos dos fármacos
Vibrio cholerae não O1/genética
Vibrio cholerae não O1/isolamento & purificação
Virulência/genética
Microbiologia da Água
Limites: Animais
Humanos
Coelhos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  7 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-506877
Autor: Lucca-Junior, W; Janovick, J. A; Conn, P. M.
Título: Participation of the endoplasmic reticulum protein chaperone thio-oxidoreductase in gonadotropin-releasing hormone receptor expression at the plasma membrane
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;42(2):164-167, Feb. 2009. graf.
Idioma: en.
Resumo: Chaperone members of the protein disulfide isomerase family can catalyze the thiol-disulfide exchange reaction with pairs of cysteines. There are 14 protein disulfide isomerase family members, but the ability to catalyze a thiol disulfide exchange reaction has not been demonstrated for all of them. Human endoplasmic reticulum protein chaperone thio-oxidoreductase (ERp18) shows partial oxidative activity as a protein disulfide isomerase. The aim of the present study was to evaluate the participation of ERp18 in gonadotropin-releasing hormone receptor (GnRHR) expression at the plasma membrane. Cos-7 cells were cultured, plated, and transfected with 25 ng (unless indicated) wild-type human GnRHR (hGnRHR) or mutant GnRHR (Cys14Ala and Cys200Ala) and pcDNA3.1 without insert (empty vector) or ERp18 cDNA (75 ng/well), pre-loaded for 18 h with 1 µCi myo-[2-3H(N)]-inositol in 0.25 mL DMEM and treated for 2 h with buserelin. We observed a decrease in maximal inositol phosphate (IP) production in response to buserelin in the cells co-transfected with hGnRHR, and a decrease from 20 to 75 ng of ERp18 compared with cells co-transfected with hGnRHR and empty vector. The decrease in maximal IP was proportional to the amount of ERp18 DNA over the range examined. Mutants (Cys14Ala and Cys200Ala) that could not form the Cys14-Cys200 bridge essential for plasma membrane routing of the hGnRHR did not modify maximal IP production when they were co-transfected with ERp18. These results suggest that ERp18 has a reduction role on disulfide bonds in wild-type hGnRHR folding.
Descritores: Membrana Celular/metabolismo
Proteína Dissulfeto Redutase (Glutationa)/metabolismo
Receptores LHRH/metabolismo
-Busserrelina/metabolismo
Busserrelina/farmacologia
Chlorocebus aethiops
Células COS
Membrana Celular/química
Fosfatos de Inositol/metabolismo
Mutação
Proteína Dissulfeto Redutase (Glutationa)/genética
Limites: Animais
Humanos
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-422502
Autor: Arias, Andrés Augusto; Dinauer, Mary C; Ding, Jiabin; Matute, Juan David; Patiño, Pablo Javier.
Título: Expresión y actividad de posibles polimorfismos provenientes de individuos normales en la proteína de 67 kd del sistema NADPH oxidasa utilizando el sistema COS phox / Expression and activity of polymorphisms in the 67-kDa protein of the NADPH oxidase system
Fonte: Biomédica (Bogotá);24(3):262-272, sept. 2004. ilus.
Idioma: es.
Resumo: El sistema NADPH oxidasa de las células fagocíticas cumple una función importante durante la respuesta antimicrobiana del organismo. La activación de este sistema está precedida por la translocación de las proteínas citosólicas p67 phox , p47 phox y p40 phox hacia la membrana para ponerse en contacto con el flavocitocromo b 558 , lo que induce la generación del anión superóxido, un precursor de agentes microbicidas oxidantes. El presente trabajo presenta un análisis funcional del sistema NADPH oxidasa basado en los hallazgos de polimorfismos encontrados en el gen de p67 phox de individuos sanos. Para esto se generaron mutaciones en el cADN que codifica la p67 phox y se expresaron en el sistema de células COS phox . Los datos obtenidos en el presente trabajo indican que los cambios Val 166 .Ile, Pro 329 .Ser y His 389 .Gln no generan alteraciones en el funcionamiento de la p67 phox cuando su función se analizó en el sistema transgénico basado en células COS-7. Por lo tanto, estos polimorfismos no generan ningún riesgo genético de producir deficiencias en la activación del sistema NADPH oxidasa. Además, se demuestra que el modelo de células COS phox representa un nuevo sistema celular, fácilmente transfectable que permite estudiar la función del sistema NADPH oxidasa de las células fagocíticas y sus particularidades genéticas. Finalmente, los hallazgos con estos polimorfismos nos permiten avanzar en el conocimiento sobre los mecanismos moleculares involucrados en la activación del sistema NADPH oxidasa células fagocíticas
Descritores: Células COS
NADPH Oxidases
Polimorfismo Genético
Proteínas/genética
-Transfecção
Transformação Genética
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


  9 / 10 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-420267
Autor: Yan, B; Wang, H; Kon, T; Li, C. Y.
Título: Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(2):169-176, Feb. 2006. tab, graf.
Idioma: en.
Resumo: We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-transfection as a screening method. In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen-activated protein kinase cascade in reporter gene assays. However, Western blots showed that the induction of Elk-1-regulated expression of endogenous c-Fos was not affected by Pim-1. The phosphorylation and activation of neither Erk1/2 nor Elk-1 was influenced by Pim-1. Also, in the gel shift assay, the pattern of endogenous NFkappaB binding to its probe was not changed in any manner by Pim-1. These data indicate that Pim-1 does not regulate the activation of Erk1/2, Elk-1 or NFkappaB. These contrasting results suggest a pitfall of the transient co-transfection reporter assay in analyzing the regulation of transcription factors outside of the chromosome context. It ensures that results from reporter gene expression assay should be verified by study of endogenous gene expression.
Descritores: Expressão Gênica/fisiologia
Genes fos/fisiologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Proteínas Proto-Oncogênicas c-pim-1/metabolismo
Ativação Transcricional
Proteínas Elk-1 do Domínio ets/metabolismo
-Western Blotting
Chlorocebus aethiops
Células COS
Indução Enzimática
Expressão Gênica/genética
Genes Reporter/genética
Genes Reporter/fisiologia
Genes fos/genética
Células HeLa
Células Jurkat
Transdução de Sinais
Ativação Transcricional
Transfecção
Proteínas Elk-1 do Domínio ets/genética
Limites: Animais
Humanos
Responsável: BR1.1 - BIREME


  10 / 10 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-303549
Autor: Goldstein, J; Silberstein, C; Ibarra, C.
Título: Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;35(2):145-151, Feb. 2002. ilus.
Idioma: en.
Projeto: CONICET.
Resumo: Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (98.5 percent) of cAMP production was observed in COS-7 cells transfected with the AC I isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with ionomycin. A high inhibition (76 percent) of cAMP production was also observed in COS-7 cells transfected with the AC VI isoform and previously treated with SNP (10 mM) for 30 min, when stimulated with forskolin. No effect on cAMP production was observed in cells transfected with AC isoforms II and V
Descritores: Adenilil Ciclases
Óxido Nítrico
Doadores de Óxido Nítrico
Nitroprussiato
-Células COS
AMP Cíclico
Isoenzimas
Rim
Mamíferos
Plasmídeos
Transfecção
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde