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Pesquisa : A11.251.800 [Categoria DeCS]
Referências encontradas : 7 [refinar]
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Id: lil-747107
Autor: Yüksel, Arif; Bilgir, Ferda; Bilgir, Oktay; Calan, Mehmet; Bozkaya, Giray.
Título: Increased circulating macrophage migration inhibitory factor levels are associated with coronary artery disease
Fonte: Clinics;70(3):169-172, 03/2015. tab.
Idioma: en.
Resumo: BACKGROUND: To evaluate the macrophage migration inhibitory factor and E-selectin levels in patients with acute coronary syndrome. MATERIALS/METHODS: We examined the plasma migration inhibitory factor and E-selectin levels in 87 patients who presented with chest pain at our hospital. The patients were classified into two groups according to their cardiac status. Sixty-five patients had acute myocardial infarction, and 22 patients had non-cardiac chest pain (non-coronary disease). We designated the latter group of patients as the control group. The patients who presented with acute myocardial infarction were further divided into two subgroups: ST-elevated myocardial infarction (n = 30) and non-ST elevated myocardial infarction (n = 35). RESULTS: We found higher plasma migration inhibitory factor levels in both acute myocardial infarction subgroups than in the control group. However, the E-selectin levels were similar between the acute myocardial infarction and control patients. In addition, we did not find a significant difference in the plasma migration inhibitory factor levels between the ST elevated myocardial infarction and NST-elevated myocardial infarction subgroups. DISCUSSION: The circulating concentrations of migration inhibitory factor were significantly increased in acute myocardial infarction patients, whereas the soluble E-selectin levels were similar between acute myocardial infarction patients and control subjects. Our results suggest that migration inhibitory factor may play a role in the atherosclerotic process. .
Descritores: /metabolismo
CHEMOKINE CCLABORTION APPLICANTS/metabolismo
Interferon gama/metabolismo
Neoplasias Mamárias Animais/imunologia
Esferoides Celulares/imunologia
Linfócitos T Citotóxicos/metabolismo
Linfócitos T Auxiliares-Indutores/metabolismo
-Alginatos
Antígenos de Neoplasias/imunologia
Antígenos de Neoplasias/metabolismo
Linhagem Celular Tumoral
Movimento Celular
Quitosana
/genética
CHEMOKINE CCLABORTION APPLICANTS/genética
/imunologia
CHEMOKINE CCLABORTION APPLICANTS/imunologia
Ácido Glucurônico
Granzimas/metabolismo
Ácidos Hexurônicos
Imunidade Celular
Interferon gama/genética
Interferon gama/imunologia
Neoplasias Mamárias Animais/genética
Neoplasias Mamárias Animais/metabolismo
Neoplasias Mamárias Animais/patologia
Esferoides Celulares/metabolismo
Esferoides Celulares/patologia
Linfócitos T Citotóxicos/imunologia
Linfócitos T Auxiliares-Indutores/imunologia
Microambiente Tumoral
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


  2 / 7 LILACS  
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Id: lil-682404
Autor: Brazilian Journal of Medical and Biological Research; Wen, Z.; Liao, Q.; Hu, Y.; You, L.; Zhou, L.; Zhao, Y..
Título: A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(7):634-642, ago. 2013. graf.
Idioma: en.
Resumo: Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Compared to two-dimensional culture of cell monolayer, three-dimensional (3-D) models more closely mimic native tissues, since the tumor microenvironment established in 3-D models often plays a significant role in cancer progression and cellular responses to the drugs. Accumulating evidence has highlighted the benefits of 3-D in vitro models of various cancers. In the present study, we have developed a spheroid-based, 3-D culture of pancreatic cancer cell lines MIAPaCa-2 and PANC-1 for pancreatic drug testing, using the acid phosphatase assay. Drug efficacy testing showed that spheroids had much higher drug resistance than monolayers. This model, which is characteristically reproducible and easy and offers rapid handling, is the preferred choice for filling the gap between monolayer cell cultures and in vivo models in the process of drug development and testing for pancreatic cancer.
Descritores: Fosfatase Ácida/metabolismo
Ensaios de Seleção de Medicamentos Antitumorais/métodos
Neoplasias Pancreáticas/tratamento farmacológico
Esferoides Celulares/efeitos dos fármacos
-Antimetabólitos Antineoplásicos/administração & dosagem
Sobrevivência Celular
Técnicas de Cultura de Células/métodos
Linhagem Celular Tumoral/efeitos dos fármacos
Linhagem Celular Tumoral/enzimologia
Desoxicitidina/administração & dosagem
Desoxicitidina/análogos & derivados
Fluoruracila/administração & dosagem
Neoplasias Pancreáticas/enzimologia
Esferoides Celulares/enzimologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  3 / 7 LILACS  
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Id: lil-618047
Autor: Li, Ying-fei; Xiao, Bing; Tu, San-fang; Wang, Yuan-yuan; Zhang, Xiao-lang.
Título: Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(3):197-204, Mar. 2012. ilus, tab.
Idioma: en.
Resumo: Our group established a method to culture spheres under serum-free culture condition. However, the biological characteristics and the tumorigenicity of spheres are unknown. Here, we demonstrate that sphere cells expressed high levels of the putative colorectal cancer stem cell markers CD133 and CD44. The CD133-positive rates were 13.27 ± 5.62, 52.71 ± 16.97 and 16.47 ± 2.45 percent in sphere cells, regular Colo205 cells and differentiated sphere cells, respectively, while the CD44-positive rates were 62.92 ± 8.38, 79.06 ± 12.10 and 47.80 ± 2.5 percent, respectively, and the CD133/CD44-double-positive rates were 10.77 ± 4.96, 46.89 ± 19.17 and 12.41 ± 2.27 percent, respectively (P < 0.05). Cancer sphere cells formed crypt-like structures in 3-D culture. Moreover, cells from cancer spheres exhibited more tumorigenicity than regular Colo205 cells in a xenograft assay. The cancer sphere cells displayed much higher oncogenicity than regular Colo205 cells to initiate neoplasms, as assayed by H&E staining, Musashi-1 staining and electron microscopy. Our findings indicated that the sphere cells were enriched with cancer stem cells (CSCs), and exhibited more proliferation capacity, more differentiation potential and especially more tumorigenicity than regular Colo205 cells in vitro and in vivo. Further isolation and characterization of these CSCs may provide new insights for novel therapeutic targets and prognostic markers.
Descritores: Antígenos CD/metabolismo
/metabolismo
ANTIGENS, CDABSENTEEISM/metabolismo
Proliferação Celular
Neoplasias do Colo/patologia
Glicoproteínas/metabolismo
Células-Tronco Neoplásicas/patologia
Peptídeos/metabolismo
Esferoides Celulares/patologia
-Biomarcadores Tumorais
Linhagem Celular Tumoral
Técnicas de Cultura de Células/métodos
Neoplasias do Colo/metabolismo
Camundongos Endogâmicos NOD
Camundongos SCID
Células-Tronco Neoplásicas/metabolismo
Esferoides Celulares/metabolismo
Células Tumorais Cultivadas
Ensaios Antitumorais Modelo de Xenoenxerto
Limites: Animais
Seres Humanos
Camundongos
Responsável: BR1.1 - BIREME


  4 / 7 LILACS  
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Id: lil-582888
Autor: Abaidoo, C. S; Warren, M. A; Andrews, P. W; Boateng, K. A.
Título: A quantitative assessment of the morphological characteristics of BeWo cells as an in vitro model of human trophoblast cells / Una evaluación cuantitativa de las características morfológicas de las células BeWo como un modelo in vitro de las células de trofoblasto humano
Fonte: Int. j. morphol;28(4):1047-1058, dic. 2010. ilus.
Idioma: en.
Resumo: In order to study the detailed morphology of trophoblast cells during human implantation, BeWo cells were cultured as spheroids in suspension culture. These cultures were then processed for light and electron microscopical examination. The present study showed that the BeWo spheroids consist of two cell types which are cytotrophoblast-like and syncytiotrophoblast-like. The cells with larger nuclear diameter made up only about 1 percent of the cell population and appear to be those of syncytiotrophoblast. Therefore the predominant cell type of the BeWo spheroids appeared to be relatively undifferentiated and cytotrophoblast-like. About 10 percent of the BeWo cells in the present study were mitotic, indicating a highly proliferative population. Total cell number increased about 12 times during the culture period from 107 +/- 9 on day 1 to 1211 +/- 145 on day 7 whereas the volume per cell increased about 2 times, from 1300 um3 on day 1 to 2400 um3 on day 7. Therefore overall growth of BeWo spheroids is due to both hyperplasia and hypertrophy. However, it appears that cell proliferation outstrips volumetric growth. These quantitative data show that BeWo cells grow mainly by hyperplasia and provide baseline values for further studies. In addition, the results show that BeWo cell morphology has marked similarities to that reported for human trophoblast, making it a useful model for subsequent in vitro studies.

En un cultivo de suspensión se estudió la morfología de las células durante la implantación del trofoblasto humano, células BeWo. Estos cultivos fueron procesados y examinados a través de microscopía de luz y electrónica. El estudio mostró que los esferoides BeWo constan de dos tipos de células, citotrofoblasto y sincitiotrofoblasto. Las células con mayor diámetro nuclear parecen ser los sincitiotrofoblasto que representaban sólo el 1 por ciento de la población celular. Por tanto, el tipo celular predominante de los esferoides BeWo parecían ser relativamente indiferenciados como citotrofoblasto. Alrededor del 10 por ciento de las células BeWo fueron mitóticas, lo que indica una población altamente proliferativa. El número de células totales aumentó alrededor de 12 veces durante el período de cultivo de 107 +/- 9 días en el día 1 a 1211 +/- 145 en el día 7, mientras que el volumen de la célula creció alrededor de 2 veces, desde 1300 mm3 el día 1 hasta 2400 mm3 el día 7. Por lo tanto, el crecimiento global de esferoides BeWo se debe tanto a la hiperplasia como a la hipertrofia. Sin embargo, parece que la proliferación celular supera al crecimiento volumétrico. Estos datos cuantitativos muestran que las células BeWo crecen principalmente por hiperplasia y proporcionan valores de referencia para estudios posteriores. Además, los resultados muestran que la morfología celular BeWo ha marcado similitudes con los reportado para el trofoblasto humano, por lo que es un modelo útil para posteriores estudios in vitro.
Descritores: Trofoblastos/ultraestrutura
-Meios de Cultura
Esferoides Celulares/ultraestrutura
Microscopia Eletrônica
Proliferação Celular
Fatores de Tempo
Limites: Seres Humanos
Responsável: CL1.1 - Biblioteca Central


  5 / 7 LILACS  
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Id: lil-440721
Autor: Lorenti, Alicia S; Hidalgo, Alejandra; Barbich, Mariana R; Torres, José; Batalle, Juan; Izaguirre, María F; Fiorucci, María Paula; Casco, Víctor; Gadano, Adrián; Argibay, Pablo F.
Título: Análisis de la polaridad estructural y funcional de estructuras tridimensionales (esferoides) de células hepáticas porcinas cultivadas in vitro / Structural and functional polarity of porcine hepatocyte cultured spheroids
Fonte: Acta gastroenterol. latinoam;36(2):66-75, jun. 2006. graf, ilus.
Idioma: es.
Resumo: Los hepatocitos son células epiteliales polarizadas que, al ser aisladas y cultivadas, pierden la polaridad y las propiedades de célula diferenciada. El cultivo de células hepáticas como esferoides permite obtener estructuras con organización de tipo tisular. En este trabajo se analizó estructural y funcionalmente la polaridad de esferoides porcinos. Para ello, las células hepáticas porcinas fueron aisladas y cultivadas en agitación constante. La actividad metabólica de los esferoides fue probada mediante el metabolismo de diazepam y de amonio, así como con síntesis de albúmina. Sus características estructurales mostraron la polaridad de las células. Fueron observados paquetes de fibras de colágeno distribuidas irregularmente y fibras reticulares en formahomogénea en todo el volumen del esferoide. Se hallaron células Ck19+ formando estructuras tipo ducto biliar, así como también _ y _-cateninas y pan-cadherinas en diferentes zonas, especialmente en las laminas externas, con características de epitelio cuboidal. Por microscopía electrónica de barrido se observaron estructuras muy compactas con superficie lisa, y por microscopía electrónica de transmisión, canalículos biliares con microvellosidades, uniones tight, zonula adherens y desmosomas. Las organelas celulares como mitocondrias, núcleos, nucleolos, peroxisomas, retículo endoplásmico estaban bien conservadas. Una compleja red de canalículos biliares fue observada mediante la incorporación y excreción de un análogo de sal biliar fluorescente. El análisis de los ácidos biliares excretados mostró un patrón normal. La morfología y funcionalidad de los esferoides puede aportar un modelo apropiado para aplicaciones en las que es primordial mantener las funciones específicas del hígado, como un dispositivo de hígado bioartificial.

Hepatocytes are epithelial cells that show a complex polarity in vivo. However, hepatocytes isolated and cultured in vitro normally lose both their differentiated properties and polarity. Culturing hepatocyte spheroids seems to be the accurate approach to maintain tissue level of organization. The structural and functional polaritiesof pig liver spheroids were analyzed in this work. Swine liver cells were isolated and cultured as spheroids. Their metabolic activity was proved through the metabolism of diazepam, ammonium and synthesis of albumin. Several structural features show the presence of polarity in the cells inside the spheroids. Reticular and collagen fibers, as well as Ck19(+) cells forming duct-like structures were found. _eta and _-catenins and pancadherins were positive in different regions of the spheroids, mainly in the outer cell layers, which have cuboidal epithelia features. The scanning electron microscopy showed a tightly compacted architecture, with smooth surface. The transmission electron microscopy analysis showed bile canaliculi with microvilli, tight junctions, zonula adherens and desmosome-like junctions. Wellmaintained cellular organelles, as mitochondria, nucleus,nucleolus, peroxisomes, endoplasmic reticulum, were seen in the spheroids. A complex inner bile canaliculinetwork was shown by using a fluorescent bile acid analogue incorporated and excreted by the spheroids. Furthermore, excretion of a normal pattern of bile acids was demonstrated. The morphology and functionality of the spheroids may provide an appropriate model for applications where the maintenance of liver-specific functions is crucial, as a bioartificial liver device.
Descritores: Polaridade Celular/fisiologia
Hepatócitos/citologia
Hepatócitos/fisiologia
Esferoides Celulares/citologia
Esferoides Celulares/fisiologia
-Albuminas/metabolismo
Diazepam/metabolismo
Imunofluorescência
Hepatócitos/metabolismo
Imuno-Histoquímica
Microscopia Eletrônica
Suínos
Esferoides Celulares/metabolismo
Ureia/metabolismo
Limites: Animais
Responsável: BR1.1 - BIREME


  6 / 7 LILACS  
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Borojevic, R
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Id: lil-409275
Autor: Rossi, M. I. D; Barros, A. P. D. N; Baptista, L. S; Garzoni, L. R; Meirelles, M. N; Takiya, C. M; Pascarelli, B. M. O; Dutra, H. S; Borojevic, R.
Título: Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(10):1455-1462, Oct. 2005. graf.
Idioma: en.
Conferência: Apresentado em: SIMEC 2004 (International Symposium on Extracellular Matrix), Angra dos Reis, September 27-30, 2004.
Projeto: Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Programa Avançado de Biologia Celular Aplicado à Medicina.
Resumo: Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis takes place inside the bone marrow cavity where hematopoietic cells are intimately associated with a specialized three 3-D scaffold of stromal cell surfaces and extracellular matrix that comprise specific niches. The relationship between hematopoietic cells and their niches is highly dynamic. Under steady-state conditions, hematopoietic cells migrate within the marrow cavity and circulate in the bloodstream. The mechanisms underlying hematopoietic stem/progenitor cell homing and mobilization have been studied in animal models, since conventional two-dimensional (2-D) bone marrow cell cultures do not reproduce the complex 3-D environment. In this review, we will highlight some of the mechanisms controlling hematopoietic cell migration and 3-D culture systems.
Descritores: Células da Medula Óssea/citologia
Técnicas de Cultura de Células/métodos
Movimento Celular/fisiologia
Células-Tronco Hematopoéticas/fisiologia
Esferoides Celulares/fisiologia
-Células Estromais/fisiologia
Limites: Animais
Seres Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  7 / 7 LILACS  
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Id: lil-303556
Autor: Jianmin, Z; Hongfang, W; Meifu, F.
Título: Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;35(2):255-260, Feb. 2002. ilus, tab, graf.
Idioma: en.
Resumo: The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were organized into several layers. Intercellular spaces were 0.5-2.0 æm wide and filled with many microvilli. The percent of viable cells was 87 percent. The cells cultured as multicellular aggregates were resistant to pharmorubicin with IC50 4.5-fold and 7.7-fold that of monolayer culture as determined by flow cytometry and MTT assay, respectively. This three-dimensional culture model may be used to investigate the mechanisms of multicellular drug resistance of hepatocarcinoma and to screen new anticancer drugs
Descritores: Antibióticos Antineoplásicos
Carcinoma Hepatocelular
Epirubicina
Neoplasias Hepáticas
Esferoides Celulares
-Antibióticos Antineoplásicos
Antineoplásicos
Agregação Celular
Técnicas de Cultura de Células
Linhagem Celular
Sobrevivência Celular
Resistência a Medicamentos Antineoplásicos
Epirubicina
Citometria de Fluxo
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Esferoides Celulares
Limites: Seres Humanos
Masculino
Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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