Base de dados : LILACS
Pesquisa : A11.270 [Categoria DeCS]
Referências encontradas : 14 [refinar]
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Id: biblio-974343
Autor: Nuanpeng, Sunan; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
Título: Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices
Fonte: Braz. j. microbiol;49(supl.1):140-150, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20 × 20 × 5 mm3 is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54 g/L and 1.36 g/L h, respectively. The use of the ALM-immobilized cells was successful for at least six consecutive batches (360 h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.
Descritores: Saccharomyces cerevisiae/metabolismo
Microbiologia Industrial/métodos
Sorghum/microbiologia
Etanol/metabolismo
-Saccharomyces cerevisiae/química
Células Imobilizadas/metabolismo
Células Imobilizadas/química
Sorghum/metabolismo
Sorghum/química
Etanol/análise
Alginatos/química
Fermentação
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  2 / 14 LILACS  
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Id: biblio-889238
Autor: Przystaś, Wioletta; Zabłocka-Godlewska, Ewa; Grabińska-Sota, Elżbieta.
Título: Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports
Fonte: Braz. j. microbiol;49(2):285-295, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: Ministry of Science and Higher Education.
Resumo: Abstract Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization. The best growth of fungal strains was observed on the washer, brush, grid and sawdust supports. Based on the results of dye adsorption, the brush and the washer were selected for further study. These solid supports adsorbed dyes at a negligible level, while the sawdust adsorbed 82.5% of brilliant green and 19.1% of Evans blue. Immobilization of biomass improved dye removal. Almost complete decolorization of diazo dye Evans blue was reached after 24 h in samples of all strains immobilized on the washer. The process was slower when the brush was used for biomass immobilization. Comparable results were reached for brilliant green in samples with biomass of strains BWPH and RWP17. High decolorization effectiveness was reached in samples with dead fungal biomass. Intensive removal of the dyes by biomass immobilized on the washer corresponded to a significant decrease in phytotoxicity and a slight decrease in zootoxicity of the dye solutions. The best decolorization results as well as reduction in toxicity were observed for the strain P. picipes (RWP17).
Descritores: Basidiomycota/metabolismo
Poluentes Químicos da Água/metabolismo
Corantes/metabolismo
-Compostos Azo/metabolismo
Compostos de Tritil/metabolismo
Biotransformação
Células Imobilizadas/metabolismo
Adsorção
Águas Residuárias
Responsável: BR1.1 - BIREME


  3 / 14 LILACS  
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Id: biblio-889145
Autor: Dong, Yuwei; Zhang, Yanqiu; Tu, Baojun.
Título: Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate
Fonte: Braz. j. microbiol;48(3):515-521, July-Sept. 2017. graf.
Idioma: en.
Projeto: Collegiate Natural Science Found of Jiangsu Province.
Resumo: Abstract Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30 °C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times. The ammonia nitrogen removal rate of the immobilized ammonia-oxidizing bacteria reached 90.30% under the optimal immobilization conditions. When compared with ammonia-oxidizing bacteria immobilized by sodium alginate alone, the bacteria immobilized by PVA and sodium alginate were superior with respect to pH resistance, the number of reuses, material cost, heat resistance, and ammonia oxidation ability.
Descritores: Bactérias/química
Técnicas Microbiológicas/métodos
Amônia/metabolismo
-Oxirredução
Álcool de Polivinil/química
Temperatura
Bactérias/metabolismo
Técnicas Microbiológicas/economia
Técnicas Microbiológicas/instrumentação
Células Imobilizadas/metabolismo
Células Imobilizadas/química
Ácido Glucurônico/química
Alginatos/química
Ácidos Hexurônicos/química
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  4 / 14 LILACS  
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Id: biblio-1010289
Autor: Stasiak-Rózanska, Lidia; Blazejak, Stanislaw; Gientka, Iwona; Bzducha-Wróbel, Anna; Lipinska, Edyta.
Título: Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract
Fonte: Electron. j. biotechnol;27:44-48, May. 2017. tab.
Idioma: en.
Resumo: Background: Depletion of petroleum resources has enforced the search for alternative sources of renewable energy. Introduction of biofuels into the market was expected to become a solution to this disadvantageous situation. Attempts to cover fuel demand have, however, caused another severe problem­the waste glycerol generated during biodiesel production at a concentration of approximately 10% w/w. This, in turn, prompted a global search for effective methods of valorization of the waste fraction of glycerol. Results: Utilization of the waste fraction at 48 h with an initial glycerol concentration of 30 g·L-1 and proceeding with 62% efficiency enabled the production of 9 g·L-1 dihydroxyacetone at 50% substrate consumption. The re-use of the immobilized biocatalyst resulted in a similar concentration of dihydroxyacetone (8.7 g·L-1) in two-fold shorter time, with an efficiency of 85% and lower substrate consumption (35%). Conclusions: The method proposed in this work is based on the conversion of waste glycerol to dihydroxyacetone in a reaction catalyzed by immobilized Gluconobacter oxydans cell extract with glycerol dehydrogenase activity, and it could be an effective way to convert waste glycerol into a valuable product.
Descritores: Células Imobilizadas/metabolismo
Di-Hidroxiacetona/metabolismo
Glicerol/metabolismo
-Resíduos
Extratos Celulares
Células Imobilizadas/química
Gluconobacter oxydans
Biocombustíveis
Reciclagem
Energia Renovável
Glicerol/química
Responsável: CL1.1 - Biblioteca Central


  5 / 14 LILACS  
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Id: biblio-889215
Autor: Singh, Utkarsh; Arora, Naveen Kumar; Sachan, Preeti.
Título: Simultaneous biodegradation of phenol and cyanide present in coke-oven effluent using immobilized Pseudomonas putida and Pseudomonas stutzeri
Fonte: Braz. j. microbiol;49(1):38-44, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Discharge of coke-oven wastewater to the environment may cause severe contamination to it and also threaten the flora and fauna, including human beings. Hence before dumping it is necessary to treat this dangerous effluent in order to minimize the damage to the environment. Conventional technologies have inherent drawbacks however, biological treatment is an advantageous alternative method. In the present study, bacteria were isolated from the soil collected from the sites contaminated by coke-oven effluent rich in phenol and cyanide. Nucleotides sequence alignment and phylogenetic analysis showed the identity of the selected phenol and cyanide degrading isolates NAUN-16 and NAUN-1B as Pseudomonas putida and Pseudomonas stutzeri, respectively. These two isolates tolerated phenol up to 1800 mg L-1 and cyanide up to 340 mg L-1 concentrations. The isolates were immobilized on activated charcoal, saw dust and fly ash. The effluent was passed through the column packed with immobilized cells with a flow rate of 5 mL min-1. The isolates showed degradation of phenol up to 80.5% and cyanide up to 80.6% and also had the ability to reduce biological oxygen demand, chemical oxygen demand and lower the pH of effluent from alkaline to near neutral. The study suggests the utilization of such potential bacterial strains in treating industrial effluent containing phenol and cyanide, before being thrown in any ecosystem.
Descritores: Cianetos/metabolismo
Fenol/metabolismo
Pseudomonas putida/metabolismo
Pseudomonas stutzeri/metabolismo
Eliminação de Resíduos Líquidos/métodos
Águas Residuárias/microbiologia
-Biodegradação Ambiental
Células Imobilizadas/classificação
Células Imobilizadas/metabolismo
Coque/análise
Cianetos/análise
Resíduos Industriais/análise
Fenol/análise
Filogenia
Pseudomonas putida/classificação
Pseudomonas putida/genética
Pseudomonas putida/isolamento & purificação
Pseudomonas stutzeri/classificação
Pseudomonas stutzeri/genética
Pseudomonas stutzeri/isolamento & purificação
Águas Residuárias/análise
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-755816
Autor: Tallur, Preeti N.; Mulla, Sikandar I.; Megadi, Veena B.; Talwar, Manjunatha P.; Ninnekar, Harichandra Z..
Título: Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1
Fonte: Braz. j. microbiol;46(3):667-672, July-Sept. 2015. ilus.
Idioma: en.
Resumo:

Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken cultures. PUF-immobilized cells showed higher degradation of cypermethrin (10 mM and 20 mM) than freely suspended cells and cells immobilized in other matrices. The PUF-immobilized cells of Micrococcus sp. strain CPN 1 were retain their degradation capacity. Thus, they can be reused for more than 32 cycles, without losing their degradation capacity. Hence, the PUF-immobilized cells of Micrococcus sp. could potentially be used in the bioremediation of cypermethrin contaminated water.

.
Descritores: Biodegradação Ambiental
Células Imobilizadas/metabolismo
Inseticidas/metabolismo
Micrococcus/metabolismo
Piretrinas/metabolismo
-Alginatos
Ácido Glucurônico
Ácidos Hexurônicos
Micrococcus/classificação
Poliuretanos
Responsável: BR1.1 - BIREME


  7 / 14 LILACS  
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Id: lil-727022
Autor: Ribeiro, Joyce Benzaquem; Ramos, Aline de Souza; Lopes, Raquel de Oliveira; Silva, Gabriela Veloso Vieira da; Souza, Rodrigo Octavio Mendonça Alves de.
Título: Whole cells in enantioselective reduction of benzyl acetoacetate
Fonte: Braz. j. microbiol;45(3):929-932, July-Sept. 2014. ilus, tab.
Idioma: en.
Resumo: The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
Descritores: Acetoacetatos/metabolismo
Compostos de Benzil/metabolismo
Kluyveromyces/metabolismo
Pichia/metabolismo
-Células Imobilizadas/metabolismo
Oxirredução
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-676907
Autor: Zhang, Pingping; Zhou, Wei; Wang, Peng; Wang, Li; Tang, Mingli.
Título: Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG
Fonte: Braz. j. microbiol;44(1):189-195, 2013. graf, tab.
Idioma: en.
Projeto: Natural Science Foundation of Anhui Province of China.
Resumo: Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 ºC. The alginate beads had well stability, retained 85% ability of enzyme production even after 7 cycles of repeated batch fermentation. These results showed the immobilization technique was a feasible and economical method for chitosansase production by Gongronella sp. JG.
Descritores: Alginatos
Crustáceos/enzimologia
Crustáceos/microbiologia
Fermentação
Fungos Aquáticos/análise
Poliuretanos/análise
Quitosana/análise
Quitosana/isolamento & purificação
Sódio/análise
-Atenção
Células Imobilizadas
Ativação Enzimática
Amostras de Alimentos
Métodos
Parâmetros
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-665837
Autor: Behera, Shuvashish; Mohanty, Rama C; Ray, Ramesh C.
Título: Ethanol fermentation of sugarcane molasses by Zymomonas mobilis MTCC 92 immobilized in Luffa cylindrica L. sponge discs and Ca-alginate matrices
Fonte: Braz. j. microbiol;43(4):1499-1507, Oct.-Dec. 2012. graf, tab.
Idioma: en.
Projeto: UGC.
Resumo: Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and Ca-alginate entrapped Z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. There was no statistical significant difference (Fischer's LSD) in sugar utilization (t = 0.254, p <0.801) and ethanol production (t =-0.663, p <0.513) between the two immobilization matrices used. Further, the immobilized cells in both the matrices were physiologically active for three more cycles of operation with less than 15 % decrease in ethanol yield in the 4th cycle, which was due to some leakage of cells. In conclusion, luffa sponge was found to be equally good as Ca-alginate as a carrier material for bacterial (Z. mobilis. cell immobilization for ethanol production. Further, it has added advantages such as it is cheap, non-corrosive and has no environmental hazard.
Descritores: Ativadores de Enzimas
Etanol/análise
Fermentação
Luffa/crescimento & desenvolvimento
Melaço/análise
Zymomonas/isolamento & purificação
-Células Imobilizadas
Métodos
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-640529
Autor: Kaymaz, Yasin; Babaoglu, Anil; Pazarlioglu, Nurdan Kasikara.
Título: Biodegradation kinetics of o-cresol by Pseudomonas putida DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system
Fonte: Electron. j. biotechnol;15(1):3-3, Jan. 2012. ilus, tab.
Idioma: en.
Resumo: The biodegradation kinetics of o-cresol was examined by acclimatized P. putida DSM 548 (pJP4) in batch experiments at varying initial o-cresol concentrations (from 50 to 500 mg/L). The kinetic parameters of o-cresol aerobic biodegradation were estimated by using the Haldane substrate inhibition equation. The biodegradation kinetics of o-cresol was investigated. In batch culture reactors, the Maximum specific growth rate (μmax), Monod constant (Ks) and the inhibition constant (Ki) were established as 0.519 h-1, 223.84 mg/L and 130.883 mg/L, respectively. o-cresol biodegradation in a batch-recirculation bioreactor system by immobilized P. putida was also studied. The recycled packed bed reactor system, which was composed of Ca-alginate beads and pumice on which cells immobilized, has been performed to determine possible stability for further developments.
Descritores: Biodegradação Ambiental
Cresóis/metabolismo
Pseudomonas putida/química
-Reatores Biológicos
Células Imobilizadas
Fenóis/metabolismo
Cinética
Responsável: CL1.1 - Biblioteca Central



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