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Id: biblio-1045993
Autor: Zeeshan, Nadia; Naz, Saher; Naz, Shumaila; Afroz, Amber; Zahur, Muzna; Zia, Safia.
Título: Heterologous expression and enhanced production of ß-1, 4-glucanase of Bacillus halodurans C-125 in Escherichia coli
Fonte: Electron. j. biotechnol;34:29-36, july. 2018. ilus, tab, graf.
Idioma: en.
Projeto: Higher Education Commission, Pakistan (HEC).
Resumo: Background: Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-ß-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study was aimed at enhancing the production of endo-1, 4-ß-glucanases (Egl) of Bacillus halodurans in Escherichia coli. Results: A putative Egl gene of Bacillus Halodurans was expressed in E. coli by cloning in pET 22b (+). On induction with isopropyl-b-D-1-thiogalactopyranoside, the enzyme expression reached upto ~20% of the cell protein producing 29.2 mg/liter culture. An increase in cell density to 12 in auto-inducing LB medium (absorbance at 600 nm) enhanced ß-glucanase production up to 5.4 fold. The molecular mass of the enzyme was determined to be 39 KDa, which is nearly the same as the calculated value. Protein sequence was analyzed by CDD, Pfam, I TASSER, COACH, PROCHECK Servers and putative amino acids involved in the formation of catalytic, substrate and metal binding domains were identified. Phylogenetic analysis of the ß-glucanases of B. halodurans was performed and position of Egl among other members of the genus Bacillus producing endo-glucanases was determined. Temperature and pH optima of the enzyme were found to be 60°C and 8.0, respectively, under the assay conditions. Conclusion: Production of endo-1, 4 ß-glucanase enzymes from B. halodurans increased several folds when cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 ß-glucanases from B. halodurans.
Descritores: Bacillus/enzimologia
Celulases/biossíntese
-Temperatura
Estabilidade Enzimática
Expressão Gênica
Parede Celular/enzimologia
Reação em Cadeia da Polimerase
Clonagem Molecular
Celulases/isolamento & purificação
Celulases/metabolismo
Escherichia coli/metabolismo
Células Vegetais/enzimologia
Concentração de Íons de Hidrogênio
Hidrólise
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-843173
Autor: Ramos, Araceli M; Gally, Marcela; Szapiro, Gala; Itzcovich, Tatiana; Carabajal, Maira; Levin, Laura.
Título: Crecimiento in vitro y producción de enzimas degradadoras de pared celular vegetal de aislamientos argentinos de Macrophomina phaseolina, agente causal de la podredumbre carbonosa en maíz / In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn
Fonte: Rev. argent. microbiol;48(4):267-273, dic. 2016. graf, tab.
Idioma: en.
Resumo: Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes --#91;pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase--#93; by several Argentinean isolates of M. phaseolina, and assessed the pathogenicity of these isolates as a preliminary step to establish the role of these enzymes in M. phaseolina-maize interaction. The isolates were grown in liquid synthetic medium supplemented with glucose, pectin, carboxymethylcellulose or xylan as carbon sources and/or enzyme inducers and glutamic acid as nitrogen source. Pectinases were the first cell wall-degrading enzymes detected and the activities obtained (polygalacturonase activity was between 0.4 and 1.3 U/ml and polymethylgalacturonase between 0.15 and 1.3 U/ml) were higher than those of cellulases and xylanases, which appeared later and in a lesser magnitude. This sequence would promote initial tissue maceration followed by cell wall degradation. Laccase was detected in all the isolates evaluated (activity was between 36 U/l and 63 U/l). The aggressiveness of the isolates was tested in maize, sunflower and watermelon seeds, being high on all the plants assayed. This study reports for the first time the potential of different isolates of M. phaseolina to produce plant cell wall-degrading enzymes in submerged fermentation.

Macrophomina phaseolina es un fitopatógeno polífago, causante de podredumbre carbonosa. Los daños que genera en cultivos de soja y maíz bajo siembra directa en Argentina, en períodos secos y calurosos, se incrementaron por su habilidad para sobrevivir como esclerocios en suelos y restos de cosecha. El propósito del trabajo fue estudiar la producción in vitro de enzimas degradadoras de pared celular vegetal (pectinasas --#91;poligalacturonasa y polimetilgalacturonasa--#93;; celulasas --#91;endoglucanasa--#93;; hemicelulasas --#91;endoxilanasa--#93; y la enzima ligninolítica lacasa) de varios aislamientos argentinos de M. phaseolina y evaluar la patogenicidad de esos aislamientos, como paso preliminar para establecer el papel de estas enzimas en la interacción M. phaseolina-maíz. Se estudió la cinética de crecimiento del hongo y la de la producción de dichas enzimas en medios de cultivo líquidos sintéticos con ácido glutámico como fuente de nitrógeno y con pectina, carboximetilcelulosa (CMC) o xilano como fuentes de carbono. Las pectinasas fueron las primeras enzimas detectadas y los máximos títulos registrados (1,4 UE/ml --#91;poligalacturonasa--#93; y 1,2 UE/ml --#91;polimetilgalacturonasa--#93;, respectivamente) superaron a los de celulasas y xilanasas, que aparecieron más tardíamente y en menor magnitud. Esta secuencia promovería la maceración inicial del tejido, seguida luego por la degradación de la pared celular vegetal. Se detectó actividad lacasa en todos los aislamientos (36 a 63 U/l). La agresividad de todos los aislamientos resultó alta en los 3 hospedantes evaluados: semillas de maíz, de girasol y de melón. En este trabajo se investiga por primera vez el potencial de distintos aislamientos de M. phaseolina para producir enzimas degradadoras de pared celular vegetal en cultivo líquido.
Descritores: Técnicas In Vitro/métodos
Parede Celular/enzimologia
Zea mays/enzimologia
Zea mays/parasitologia
-Poligalacturonase/isolamento & purificação
Celulase/isolamento & purificação
Endo-1,4-beta-Xilanases/isolamento & purificação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1047768
Autor: Baranzan Wayah, Samson; Philip, Koshy.
Título: Purification, characterization, mode of action, and enhanced production of Salivaricin mmaye1, a novel bacteriocin from Lactobacillus salivarius SPW1 of human gut origin
Fonte: Electron. j. biotechnol;35:39-47, sept. 2018. graf, tab.
Idioma: en.
Projeto: High Impact ResearchMalaysian Ministry of Higher Education grant designated as UM; . Postgraduate Research Grant.
Resumo: Background: Emergence of antibiotic resistance among pathogenic and food spoilage bacteria such as Staphylococcus aureus, Micrococcus luteus, Streptococcus pyogenes, Streptococcus sanguinis, Streptococcus mutans, Bacillus cereus, and Listeria monocytogenes triggered the search for alternative antimicrobials. An investigation aimed at purifying, characterizing, elucidating the mode of action, and enhancing the production of salivaricin from Lactobacillus salivarius of human gut origin was conducted. Results: Salivaricin mmaye1 is a novel bacteriocin purified from L. salivarius isolated from human feces. It is potent at micromolar concentrations and has a molecular weight of 1221.074 Da as determined by MALDI-TOF mass spectrometry. It has a broad spectrum of antibacterial activity. Salivaricin mmaye1 showed high thermal and chemical stability and moderate pH stability. The proteinaceous nature of salivaricin mmaye1 was revealed by the complete loss of activity after treatment with pepsin, trypsin, α-chymotrypsin, protease, and proteinase. Salivaricin mmaye1 is cell wall associated, and adsorption­desorption of the bacteriocin from the cell wall of the producer by pH modification proved successful. It exhibited a bactericidal mode of action mediated by pore formation. Its biosynthesis is regulated by a quorum sensing mechanism. Enhanced production of salivaricin mmaye1 was achieved in a newly developed growth medium. Conclusions: A novel, cell wall adhering, highly potent bacteriocin with a broad spectrum of inhibitory activity, membrane-permeabilizing ability, and enhanced production in a newly constituted medium has been isolated. It has a quorum sensing regulatory system and possesses interesting physicochemical characteristics favoring its future use in food biopreservation. These findings pave the way for future evaluation of its medical and food applications.
Descritores: Bacteriocinas/biossíntese
Bacteriocinas/química
Lactobacillus salivarius/metabolismo
-Bactérias/crescimento & desenvolvimento
Bacteriocinas/isolamento & purificação
Resistência Microbiana a Medicamentos
Testes de Sensibilidade Microbiana
Parede Celular
Percepção de Quorum
Estabilidade Proteica
Fezes/microbiologia
Concentração de Íons de Hidrogênio
Intestinos/microbiologia
Antibacterianos/química
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-842818
Autor: Queiroz, Adriano; Riley, Lee W.
Título: Bacterial immunostat: mycobacterium tuberculosis lipids and their role in the host immune response
Fonte: Rev. Soc. Bras. Med. Trop;50(1):9-18, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Resumo: Abstract: The lipid-rich cell wall of Mycobacterium tuberculosis is a dynamic structure that is involved in the regulation of the transport of nutrients, toxic host-cell effector molecules, and anti-tuberculosis drugs. It is therefore postulated to contribute to the long-term bacterial survival in an infected human host. Accumulating evidence suggests that M. tuberculosis remodels the lipid composition of the cell wall as an adaptive mechanism against host-imposed stress. Some of these lipid species (trehalose dimycolate, diacylated sulphoglycolipid, and mannan-based lipoglycans) trigger an immunopathologic response, whereas others (phthiocerol dimycocerosate, mycolic acids, sulpholipid-1, and di-and polyacyltrehalose) appear to dampen the immune responses. These lipids appear to be coordinately expressed in the cell wall of M. tuberculosis during different phases of infection, ultimately determining the clinical fate of the infection. This review summarizes the current state of knowledge on the metabolism, transport, and homeostatic or immunostatic regulation of the cell wall lipids, and their orchestrated interaction with host immune responses that results in bacterial clearance, persistence, or tuberculosis.
Descritores: Parede Celular/metabolismo
Lipídeos/fisiologia
Mycobacterium tuberculosis/fisiologia
-Proteínas de Membrana Transportadoras
Parede Celular/fisiologia
Metabolismo dos Lipídeos
Imunidade Inata
Lipídeos de Membrana/fisiologia
Mycobacterium tuberculosis/metabolismo
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-985162
Autor: Winarsih, Sri; Kosasih, Tomson; Putera, Marvin Anthony; Rahmadhiani, Nayla; Poernomo, Erlien Lindawati; Runtuk, Kresna Septiandy; Oswari, Melissa Valensia.
Título: Β-Glucan of candida albicans cell wall extract inhibits salmonella typhimurium colonization by potentiating cellular immunity (cd8 + and cd4 + t cells)
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180254, 2019. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Antimicrobial resistance has been reported in the drugs used for the treatment of typhoid fever. The immunomodulatory substance β-glucan can be used as an alternative therapy as it potentiates host immunity. The aims of this study are to observe the effect of Candida albicans cell wall (CCW) extract towards host immunity (TCD8+ and TCD4+ cells in spleen, intestinal sIgA) and its capacity to kill Salmonella in the intestine and liver of typhoid fever mice models. METHODS: Typhoid fever mice models were created by infecting mice with S. Typhimurium orally. Mice were divided into four groups: the Non-Infected, Infected, CCW (infected mice treated with 300 µg CCW extract/mouse once a day), and Ciprofloxacin groups (infected mice treated with 15 mg/kg BW ciprofloxacin twice a day). RESULTS: Secretory IgA (sIgA) concentrations of mice in the CCW group remained unchanged. However, their TCD4+ and TCD8+ cells increased substantially compared to those in the Non-Infected group. In the Ciprofloxacin group, sIgA concentrations increased markedly compared to those in the Non-Infected and CCW groups; TCD4+ and TCD8+ cells also increased significantly compared to those in the Infected Group, but not significant compared to those in the CCW group. Colonization of S. Typhimurium in the intestine and liver decreased significantly in the CCW and Ciprofloxacin groups compared to that in the Infected group, with the lowest reduction being found in the Ciprofloxacin group. CONCLUSIONS The inhibition of S. Typhimurium colonization by CCW is associated with the increase in TCD4+ and TCD8+ cells.
Descritores: Salmonella typhimurium/efeitos dos fármacos
Febre Tifoide/microbiologia
Candida albicans/química
beta-Glucanas/farmacologia
-Imunoglobulina A Secretora
Linfócitos T CD4-Positivos/microbiologia
Ciprofloxacina
Testes de Sensibilidade Microbiana
Parede Celular
Linfócitos T CD8-Positivos/microbiologia
Modelos Animais de Doenças
Imunidade Celular/imunologia
Intestinos/microbiologia
Fígado/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-1011393
Autor: Waterman, Melinda J; Bramley-Alves, Jessica; Miller, Rebecca E; Keller, Paul A; Robinson, Sharon A.
Título: Photoprotection enhanced by red cell wall pigments in three East Antarctic mosses
Fonte: Biol. Res;51:49, 2018. tab, graf.
Idioma: en.
Projeto: Australian Research Council Discovery Project; . Australian Antarctic Division.
Resumo: BACKGROUND: Antarctic bryophytes (mosses and liverworts) are resilient to physiologically extreme environmental conditions including elevated levels of ultraviolet (UV) radiation due to depletion of stratospheric ozone. Many Antarctic bryophytes synthesise UV-B-absorbing compounds (UVAC) that are localised in their cells and cell walls, a location that is rarely investigated for UVAC in plants. This study compares the concentrations and localisation of intracellular and cell wall UVAC in Antarctic Ceratodon purpureus, Bryum pseudotriquetrum and Schistidium antarctici from the Windmill Islands, East Antarctica. RESULTS: Multiple stresses, including desiccation and naturally high UV and visible light, seemed to enhance the incorporation of total UVAC including red pigments in the cell walls of all three Antarctic species analysed. The red growth form of C. purpureus had significantly higher levels of cell wall bound and lower intracellular UVAC concentrations than its nearby green form. Microscopic and spectroscopic analyses showed that the red colouration in this species was associated with the cell wall and that these red cell walls contained less pectin and phenolic esters than the green form. All three moss species showed a natural increase in cell wall UVAC content during the growing season and a decline in these compounds in new tissue grown under less stressful conditions in the laboratory. CONCLUSIONS: UVAC and red pigments are tightly bound to the cell wall and likely have a long-term protective role in Antarctic bryophytes. Although the identity of these red pigments remains unknown, our study demonstrates the importance of investigating cell wall UVAC in plants and contributes to our current understanding of UV-protective strategies employed by particular Antarctic bryophytes. Studies such as these provide clues to how these plants survive in such extreme habitats and are helpful in predicting future survival of the species studied.
Descritores: Pigmentos Biológicos/efeitos da radiação
Pigmentos Biológicos/metabolismo
Raios Ultravioleta
Parede Celular/efeitos da radiação
Parede Celular/metabolismo
Briófitas/efeitos da radiação
Briófitas/metabolismo
-Estações do Ano
Fatores de Tempo
Pigmentação/efeitos da radiação
Análise de Variância
Cromatografia Líquida de Alta Pressão
Espectroscopia de Infravermelho com Transformada de Fourier/métodos
Folhas de Planta/efeitos da radiação
Folhas de Planta/metabolismo
Microscopia Confocal
Briófitas/citologia
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central


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Id: lil-773065
Autor: Oliveira, Lidiane de.
Título: Estudo de características dos agentes de criptococose: genótipos, polimorfismo, melanização e suscetibilidade a antifúngicos / Study of characteristics of cryptococcosis agents; genotypes, polymorphism, melanization and antifungal susceptibility.
Fonte: São Paulo; s.n; 2015. [132] p. graf, tab, ilus.
Idioma: pt.
Tese: Apresentada a São Paulo (Estado) Secretaria da Saúde. Coordenadoria de Controle de Doenças. Programa de Pós-Graduação em Ciências para obtenção do grau de Mestre.
Resumo: Os objetivos deste trabalho foram determinar frequência e características de polimorfismo celular em isolados clínicos de Cryptococcus spp. e utilizar novo método de inóculo individualizado para teste de suscetibilidade a antifúngicos (TSA) utilizando isolados de pacientes hospitalizados. O estudo de polimorfismo analisou 112 isolados de C. neoformans (Cn) e 65 de C. gattii (Cg), empregandomicroscopia ótica e microscopia eletrônica de transmissão (MET), análise de melanização, TSA a fluconazol (FCZ) e anfotericina B (AmB) por E-test® e curvas de morte (Time Kill Curves, TK) para AmB, tipagem molecular e mating-type (MAT). O estudo do novo método empregou a carga fúngica de 6 pacientes em suas amostras de líquido cefalorraquidiano (LCR) no momento do diagnóstico,7°dia e 14°dia de terapia antifúngica para CSNC. As cepas foram classificadas segundo tipo molecular e MAT, TSA com FCZ e AMB e teste de combinação por tabuleiro do xadrez. A frequência de polimorfismo celular foi baixa (4%;7/177), mas nunca antes relatada em isolados de micoteca. Foi inédito o encontro de polimorfismo em isolados de sangue, indicando a facilidade de disseminação decélulas atípicas pela corrente sanguínea, assim como em isolado de LCR. Todos os fenótipos polimórficos apresentavam células titãs (100%; 7/7) e, em menor porcentagem, pseudohifas (43%; 3/7). O fenômeno de polimorfismo parece ser exclusivo de Cn, desde que não foi observado em Cg. A espessura da parede das células titãs apresentou a mesma variação (350 nm a 900 nm) em relação àscélulas...

Our objectives were to describe the frequency and characteristics of polymorphismamong clinical isolates of Cryptococcus spp. and use new method of individualizedinoculum for the test of susceptibility to antifungal agents (TSA) against theetiological agent of cryptococcosis of the central nervous system (CSNC) inhospitalized patients. The study of polymorphism analyzed 112 isolates of C.neoformans (Cn) and 65 of C. gattii (Cg) using optical microscopy andtransmission electron microscopy (TEM), analysis of melanization, TSA forfluconazole (FCZ) by E-Test® and time kill curves method (TK) for amphotericin B(AmB), molecular typing and mating-type (MAT). The study of new methodemployed the fungal load of 6 patients in their cerebrospinal fluid (CSF) samples atdiagnosis, 7th and 14th day of therapy for CSNC. The strains were classifiedaccording to molecular type and MAT, TSA using FCZ or AmB and drugcombination using AMB and 5-flucytosine (5FC) by chequerboard, and TK. Thefrequency of cell polymorphism was low (4%; 7/177), but never before reported inisolates of collection. The occurrence of polymorphism in blood isolates wasunprecedented, indicating the ease of dissemination of atypical cells into thebloodstream, as well as in CSF. All polymorphic phenotypes showed titan cells(100%; 7/7), and, at a lower percentage, formed pseudohyphae (43%; 3/7). Thephenomenon of polymorphism seems to be unique in Cn, since it was notobserved in Cg. The wall thickness of the titans cells presented the same variation(350 nm to 900 nm) compared to regular cells. Low melanization was observed in71.4% (5/7) of polymorphic isolates, two isolates showed both patterns ofmelanization. All isolates with regular cells showed high level of melanization. Lowsusceptibility (MIC, >8 mg/L) was observed in almost half of the isolates with titancells, indicating that polymorphism may be related to resistance to FCZ. The newmethod of TSA with individualized...
Descritores: Antifúngicos
Criptococose
Cryptococcus neoformans
Genótipo
Parede Celular
Responsável: BR91.2 - Centro de Documentação


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Mazzafera, Paulo
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Id: biblio-886905
Autor: LLERENA, JUAN P P; ARAÚJO, PEDRO; MAZZAFERA, PAULO.
Título: Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum
Fonte: An. acad. bras. ciênc;90(1):509-519, Mar. 2018. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Fundação de Amparo à Pesquisa do estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . FAPESP.
Resumo: ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great difficulty in RT-PCR reactions. Thus, we evaluated the effectiveness of different additives in the amplification of these genes. While COMT and CCoAOMT genes did not need any additives for other genes there was no amplification (HCT, F5H, 4CL and CCR) or the yield was very low (CAD and C4H). The application of supplementary cDNA was enough to overcome the non-specificity and low yield for C4H and C3H, while the addition of 0.04% BSA + 2% formamide was effective to amplify 4CL, CCR, F5H and CCR. HCT was amplified only by addition of 0.04% BSA + 2% formamide + 0.1 M trehalose and amplification of PAL was possible with addition of 2% of DMSO. Besides optimization of expression assays, the results show that additives can act independently or synergistically.
Descritores: Regulação da Expressão Gênica de Plantas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
Saccharum/genética
-Parede Celular/genética
Primers do DNA
Etanol
Lignina/biossíntese
Lignina/genética
Metiltransferases/genética
Responsável: BR1.1 - BIREME


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Id: biblio-1048810
Autor: Araújo, Octavio Gabryel; Mendes, Simone Martins; Vilella, Michelle; Silveira, Luis Cláudio Paterno; Simeone, Maria Lucia Ferreira; Fadini, Marcus Antonio Matiello; Parrella, Rafael Augusto da Costa.
Título: Resistance of bioenergy sorghum to Diatraea saccharalis (Lepidoptera: Crambidae) / Resistência de sorgo bioenergia à diatraea saccharalis (lepidoptera: crambidae)
Fonte: Biosci. j. (Online);35(4):1022-1032, july/aug. 2019. tab.
Idioma: en.
Resumo: This study evaluated the effects of the sugarcane borer Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) on cultivars of sweet and biomass sorghum for the selection of resistant cultivars. The present work consisted of two trials, with natural pest infestation. In the first one, 10 sweet sorghum cultivars were analyzed for the following variables: plant height, number of healthy and damaged internodes, gallery position and size, stem infestation level and soluble solids content (°Brix). In the second trial, it was analyzed 16 genotypes of high biomass sorghum, with the same variables above mentioned, in addition to the lignin, cellulose and hemicellulose contents. Among sweet sorghum genotypes evaluated, the genotype CMSXS647 stood out due to the traits: plant height, infestation level, gallery size and soluble solids content. Among the sorghum genotypes evaluated, CMSXS7030, CMSXS7012 and CMSXS7028 presented ideal characteristics for infestation level, plant height and number of lignocellulosic compounds. Such information, in addition to supporting the bioenergy sorghum breeding program, will assist in integrated pest management for sorghum cultivation.

Foram estudados os efeitos causados pela broca-do-colmo Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), em cultivares de sorgo sacarino e biomassa visando seleção de cultivares resistentes à praga. O presente trabalho foi constituído de dois ensaios, com infestação natural da praga. No primeiro, 10 cultivares de sorgo sacarino foram analisadas quanto às seguintes variáveis: altura das plantas, quantidade de internódios sadios e com injúrias, posição e tamanho da galeria, intensidade de infestação de colmos e teor de sólidos solúveis (°Brix). No segundo ensaio, foram analisados 16 genótipos de sorgo biomassa, com as mesmas variáveis supracitadas, além dos teores de lignina, celulose e hemicelulose. Entre os genótipos de sorgo sacarino avaliados, o genótipo CMSXS647 foi o que se destacou em função das características: altura de plantas, intensidade de infestação, tamanho de galerias e teor de sólidos solúveis. Entre os genótipos de sorgo biomassa avaliados: CMSXS7030, CMSXS7012 e CMSXS7028 apresentaram características ideais para intensidade de infestação, altura de plantas e quantidade de compostos lignocelulósico. Tais informações, além de prover o programa de melhoramento de sorgo energia podem ajudar o programa de MIP para a cultura do sorgo, uma vez que o produtor conhece a suscetibilidade dos materiais escolhidos.
Descritores: Parede Celular
Biomassa
Sorghum
Lepidópteros
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-1009164
Autor: Perini, Mauro A; Sin, Ignacio N; Martinez, Gustavo Adolfo; Civello, Pedro M.
Título: Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
Fonte: Electron. j. biotechnol;26:12-19, Mar. 2017. ilus, graf, tab.
Idioma: en.
Projeto: Agencia Nacional de Promoción Científica y Tecnológica ANPCyT.
Resumo: Background: Expansins play an important role in cell wall metabolism and fruit softening. Determination of expansin activity is a challenging problem since it depends on measuring cell wall properties by using ad hoc extensometers, a fact that has strongly restricted its study. Then, the objective of the work was to adapt a methodology to measure cell wall creep and expansin activity using a commercial texture meter, equipped with miniature tensile grips and an ad hoc cuvette of easy construction. Results: It was possible to measure hypocotyls acid growth and expansin activity in a reliable and reproducible way, using a commercial texture meter, common equipment found in laboratories of food science or postharvest technology. Expansin activity was detected in protein extracts from cucumber hypocotyls, tomato and strawberry fruits, and statistical differences in expansin activity were found in both fruit models at different ripening stages. Conclusions: The possibility of measuring expansin activity following this adapted protocol with a commercial texture meter could contribute to ease and increase the analysis of expansin in different systems, leading to a better understanding of the properties of these proteins under different experimental conditions.
Descritores: Proteínas de Plantas/metabolismo
Lycopersicon esculentum/metabolismo
Cucumis sativus/metabolismo
Fragaria/metabolismo
-Proteínas de Plantas/análise
Parede Celular/metabolismo
Hipocótilo/crescimento & desenvolvimento
Elasticidade
Frutas/metabolismo
Responsável: CL1.1 - Biblioteca Central



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