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Pesquisa : A11.284.187.178.170 [Categoria DeCS]
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Id: lil-634678
Autor: Berlemont, Renaud; Pipers, Delphine; Delsaute, Maud; Angiono, Federico; Feller, Georges; Galleni, Moreno; Power, Pablo.
Título: Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements / Explorando el metagenoma del suelo antártico como fuente de nuevas enzimas adaptadas al frío y elementos génicos móviles
Fonte: Rev. argent. microbiol;43(2):94-103, jun. 2011. ilus, graf, tab.
Idioma: en.
Projeto: FNRS; . CONICET. PIP 11220090100533.
Resumo: Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 14 amylase-, 3 protease-, and 11 cellulase-producing clones were detected by activity-driven screening, with apparent maximum activities around 35 °C for both amylolytic and lipolytic enzymes, and 35-55 °C for cellulases, as observed for other cold-adapted enzymes. However, the behavior of at least one of the studied cellulases is more compatible to that observed for mesophilic enzymes. These enzymes are usually still active at temperatures above 60 °C, probably resulting in a psychrotolerant behavior in Antarctic soils. Metagenomics allows to access novel genes encoding for enzymatic and biophysic properties from almost every environment with potential benefits for biotechnological and industrial applications. Only intI- and tnp-like genes were detected by PCR, encoding for proteins with 58-86 %, and 58-73 % amino acid identity with known entries, respectively. Two clones, BAC 27A-9 and BAC 14A-5, seem to present unique syntenic organizations, suggesting the occurrence of gene rearrangements that were probably due to evolutionary divergences within the genus or facilitated by the association with transposable elements. The evidence for genetic elements related to recruitment and mobilization of genes (transposons/integrons) in an extreme environment like Antarctica reinforces the hypothesis of the origin of some of the genes disseminated by mobile elements among "human-associated" microorganisms.

A partir de muestras de suelo antártico se obtuvo la metagenoteca PP1. Esta fue sometida a análisis funcionales y genotípicos para el aislamiento de nuevas enzimas adaptadas al frío con potenciales aplicaciones, y para la detección de elementos génicos asociados a la movilización de genes, respectivamente. Por tamizaje fenotípico se detectaron 14, 14, 3 y 11 clones productores de lipasas/esterasas, proteasas, amilasas y celulasas, respectivamente, con actividades máximas aparentes de 35 °C para las amilasas y lipasas, y de 35-55 °C para las celulasas, tal como se observó para otras enzimas adaptadas al frío. Sin embargo, una celulasa parece ser compatible con enzimas mesófilas, las que usualmente se mantienen activas hasta por sobre 60 °C. Este hecho probablemente esté asociado a un comportamiento psicrotolerante en los suelos antárticos. La metagenómica permite acceder a una nueva miríada de productos metabólicos con potenciales beneficios para aplicaciones biotecnológicas e industriales. Se detectaron los genes tipo intI y tnp por PCR, y sus productos génicos deducidos tuvieron identidades del 58 al 86 % y del 58 al 73 % con secuencias conocidas, respectivamente. Dos clones, BAC 27A-9 y BAC 14A-5, parecen presentar organizaciones sintéticas únicas, lo cual sugiere la existencia de rearreglos génicos probablemente debidos a divergencias evolutivas dentro del género o facilitados por la asociación de elementos de transposición. La evidencia de elementos génicos relacionados con el reclutamiento y la movilización de genes en ambientes extremos como la Antártida refuerza la hipótesis sobre el origen de algunos genes diseminados por elementos móviles entre los microorganismos asociados al ser humano.
Descritores: Clima Frio
Enzimas/genética
Sequências Repetitivas Dispersas/genética
Metagenoma
Microbiologia do Solo
-Adaptação Fisiológica
Sequência de Aminoácidos
Regiões Antárticas
Clonagem Molecular
Cromossomos Artificiais Bacterianos/genética
Enzimas/isolamento & purificação
Fertilizantes
Gasolina
Biblioteca Gênica
Dados de Sequência Molecular
Petróleo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Poluentes do Solo
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-595006
Autor: Talia, Paola; Greizerstein, Eduardo J; Hopp, H. Esteban; Paniego, Norma; Poggio, Lidia; Heinz, Ruth A.
Título: Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
Fonte: Biocell;35(1):19-28, Apr. 2011. ilus, tab.
Idioma: en.
Resumo: Bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect C-PRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.
Descritores: Análise de Sequência de DNA/métodos
Cromossomos de Plantas
Cromossomos Artificiais Bacterianos/genética
Helianthus/genética
Hibridização in Situ Fluorescente/métodos
-Sequência de Bases
Marcadores Genéticos
Locos de Características Quantitativas
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


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Id: lil-520031
Autor: Jardim, S. N.
Título: Comparative genomics of grasses tolerant to aluminum
Fonte: Genet. mol. res. (Online);6(4):1178-1189, 2007.
Idioma: en.
Resumo: The family Poaceae includes over 10,000 species, among which are the most economically important cereals: maize, sorghum, rice, wheat, rye, barley, and oat. These cereals are very important components of human and animal food. Although divergence of the members of this family occurred about 40 million years ago, comparative genome analyses demonstrated that gene orders among species of this family remain largely conserved, which can be very useful for understanding their roles and evolution. Even with an intricate evolutionary history in which chromosome fragments, losses and duplications have to be considered at the ploidy level, grasses present a genetic model system for comparative genomics. The availability of mapped molecular markers, rice genome sequences and BAC and EST libraries from several grass species, such as rice, wheat, sorghum, and maize, facilitates biology and phylogeny studies of this group. The value of using information from different species in modern plant genetics is unquestionable, especially in the study of traits such as tolerance to aluminum in soils, which affects plant growth and development. Comparative genomic approaches to aluminum tolerance can identify genomic regions and genes responsible for aluminum tolerance in grasses.
Descritores: Alumínio/toxicidade
Genoma de Planta
Poaceae
-Cromossomos Artificiais Bacterianos
Etiquetas de Sequências Expressas
Especificidade da Espécie
Duplicação Gênica
Genômica
Ploidias
Poaceae/classificação
Poaceae/crescimento & desenvolvimento
Poaceae/genética
Poluentes do Solo/toxicidade
Locos de Características Quantitativas
Tipo de Publ: Revisão
Responsável: BR26.1 - Biblioteca Central


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Id: lil-482089
Autor: Dorella, F. A; Fachin, M. S; Billault, A; Dias Neto, E; Soravito, C; Oliveira, S. C; Meyer, R; Miyoshi, A; Azevedo, V.
Título: Construction and partial characterization of a Corynebacterium pseudotuberculosis bacterial artificial chromosome library through genomic survey sequencing
Fonte: Genet. mol. res. (Online);5(4):653-663, 2006. tab, ilus, graf.
Idioma: en.
Projeto: Brasil. Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Brasil. CAPES; . FINEP; . Fundação de Amparo à Pesquisa do Estado de Minas Gerais.
Resumo: Corynebacterium pseudotuberculosis is a gram-positive bacterium that causes caseous lymphadenitis in sheep and goats. However, despite the economic losses caused by caseous lymphadenitis, there is little information about the molecular mechanisms of pathogenesis of this bacterium. Genomic libraries constructed in bacterial artificial chromosome (BAC) vectors have become the method of choice for clone development in high-throughput genomic-sequencing projects. Large-insert DNA libraries are useful for isolation and characterization of important genomic regions and genes. In order to identify targets that might be useful for genome sequencing, we constructed a C. pseudotuberculosis BAC library in the vector pBeloBAC11. This library contains about 18,000 BAC clones, with inserts ranging in size from 25 to 120 kb, theoretically representing a 390-fold coverage of the C. pseudotuberculosis genome (estimated to be 2.5-3.1 Mb). Many genomic survey sequences (GSSs) with homology to C. diphtheriae, C. glutamicum, C. efficiens, and C. jeikeium proteins were observed within a sample of 215 sequenced clones, confirming their close phylogenetic relationship. Computer analyses of GSSs did not detect chimeric, deleted, or rearranged BAC clones, showing that this library has low redundancy. This GSSs collection is now available for further genetic and physical analysis of the C. pseudotuberculosis genome. The GSS strategy that we used to develop our library proved to be efficient for the identification of genes and will be an important tool for mapping, assembly, comparative, and functional genomic studies in a C. pseudotuberculosis genome sequencing project that will begin this year.
Descritores: Corynebacterium pseudotuberculosis/genética
Cromossomos Artificiais Bacterianos/genética
Cromossomos Bacterianos/genética
Biblioteca Gênica
Genoma Bacteriano/genética
-Clonagem Molecular
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 7 LILACS  
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Texto completo SciELO Brasil
Caldeira, Roberta Lima
Carvalho, Omar dos Santos
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Id: lil-441243
Autor: Adema, Coen M; Luo, Mei-Zhong; Hanelt, Ben; Hertel, Lynn A; Marshall, Jennifer J; Zhang, Si-Ming; Dejong, Randall J; Kim, Hye-Ran; Kudrna, David; Wing, Rod A; Soderlund, Cari; Knight, Matty; Lewis, Fred A; Caldeira, Roberta Lima; Jannotti-Passos, Liana K; Carvalho, Omar dos Santos; Loker, Eric S.
Título: A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
Fonte: Mem. Inst. Oswaldo Cruz;101(supl.1):167-177, Oct. 2006. tab, graf.
Idioma: en.
Projeto: National Human Genome Research Institute under the BAC Library Production; . NIH; . Fiocruz.
Resumo: To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63 percent AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.
Descritores: Biomphalaria/genética
Cromossomos Artificiais Bacterianos
Biblioteca Gênica
Schistosoma mansoni/fisiologia
-Biomphalaria/classificação
Biomphalaria/parasitologia
Impressões Digitais de DNA
Interações Hospedeiro-Parasita/genética
Limites: Animais
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-417635
Autor: Tomkins, J. P; Luo, M; Fang, G. C; Main, D; Goicoechea, J. L; Atkins, M; Frisch, D. A; Page, R. E; Guzmán-Novoa, E; Yu, Y; Hunt, G; Wing, R. A.
Título: New genomic resources for the honey bee(Apis mellifera L): development of a deep-coverage BAC library and a preliminary STC database
Fonte: Genet. mol. res. (Online);1(4):306-316, Dec. 2002.
Idioma: en.
Resumo: We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size of 113 kb (range = 27 to 213 kb) and 2 empty vectors. Based on an estimated genome size of 270 Mb, this library provides approximately 15 haploid genome equivalents, allowing >99 probability of recovering any specific sequence of interest. High-density colony filters were gridded robotically using a Genetix Q-BOT in a 4 x 4 double-spotted array on 22.5-cm2 filters. Screening of the library with four mapped honey bee genomic clones and two bee cDNA probes identified an average of 21 positive signals per probe, with a range of 7-38 positive signals per probe. An additional screening was performed with nine aphid gene fragments and one Drosophila gene fragment resulting in seven of the nine aphid probes and the Drosophila probe producing positive signals with a range of 1 to 122 positive signals per probe (average of 45). To evaluate the utility of the library for sequence tagged connector analysis, 1152 BAC clones were end sequenced in both forward and reverse directions, giving a total of 2061 successful reads of high quality. End sequences were queried against SWISS-PROT, insect genomic sequence GSS, insect EST, and insect transposable element databases. Results in spreadsheet format from these searches are publicly available at the Clemson University Genomics Institute (CUGI) website in a searchable format (http://www.genome.clemson.edu/projects/stc/bee/AM__Ba/)
Descritores: Abelhas/genética
Cromossomos Artificiais Bacterianos/genética
Biblioteca Genômica
Sitios de Sequências Rotuladas
-Clonagem Molecular/métodos
Genes de Insetos/genética
Hibridização In Situ
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Limites: Animais
Tipo de Publ: Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


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Id: lil-417604
Autor: De Donato, M; Gallagher, D. S; Lehn, C; Gill, C; Taylor, J. F.
Título: Molecular cytogenetic assignment of genes to bovine chromosome 5
Fonte: Genet. mol. res. (Online);2(3):260-270, Sept. 2003.
Idioma: en.
Resumo: Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified the desired bovine genes were used for screening a bovine bacterial artificial chromosome library. The positive clones were localized to a specific band of bovine chromosome 5 by fluorescence in situ hybridization. The genes HOXC4, SP1 and IGFBP6 were localized to band q21, COL2A1 was localized to bands q21-q23, IGF1 was localized to band q26, MB to band q31 and the gene CYP2D6 was localized to band q35. The cytogenetic assignment of SP1, IGFBP6, COL2A1, IGF1, MB and CYP2D6 is first reported here and the assignment of HOXC4 refines the previous assignment of this gene. The identification and localization of these genes further support the development of the human to bovine comparative map through characterizing the homologous segments conserved in the evolution of these species. This information will be useful for the future localization of genes that affect economically important traits in bovines
Descritores: Bovinos/genética
Mapeamento Cromossômico/veterinária
Característica Quantitativa Herdável
-Análise de Sequência de DNA/métodos
Análise de Sequência de DNA/veterinária
Cromossomos Artificiais Bacterianos/genética
Hibridização in Situ Fluorescente
Mapeamento Cromossômico/métodos
Reação em Cadeia da Polimerase
Limites: Humanos
Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME



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