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Pesquisa : A11.284.187.190 [Categoria DeCS]
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Id: lil-666803
Autor: Cavalcante, Fernanda Sampaio; Schuenck, Ricardo Pinto; Caboclo, Roberta Mello Ferreira; Ferreira, Dennis de Carvalho; Nouér, Simone Aranha; Santos, Kátia Regina Netto dos.
Título: Tetracycline and trimethoprim/sulfamethoxazole at clinical laboratory: Can they help to characterize Staphylococcus aureus carrying different SCCmec types?
Fonte: Rev. Soc. Bras. Med. Trop;46(1):100-102, Jan.-Feb. 2013. tab.
Idioma: en.
Resumo: INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) can be difficult to detect at the clinical practice. METHODS: We analyzed 140 MRSA isolates from inpatients to correlate the antimicrobial susceptibility with the SCCmec types. RESULTS: Type III (n = 63) isolates were more resistant to ciprofloxacin, clindamycin, cloramphenicol, erythromycin, gentamicin, and rifampin than type IV (n = 65) ones (p < 0.05). Moreover, type IV isolates were susceptible to tetracycline (100%) and trimethoprim/sulfamethoxazole (98%), while type III isolates presented resistance to them. CONCLUSIONS: In regions where these SCCmec types are prevalent, the detection of specific resistant phenotypes could help to predict them, mainly when there are no technical conditions to SCCmec typing.
Descritores: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Staphylococcus aureus Resistente à Meticilina/genética
Tetraciclina/farmacologia
Combinação Trimetoprima e Sulfametoxazol/farmacologia
-Cromossomos Bacterianos/genética
DNA Bacteriano/genética
Genótipo
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana/métodos
Fenótipo
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 10 LILACS  
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Id: lil-482089
Autor: Dorella, F. A; Fachin, M. S; Billault, A; Dias Neto, E; Soravito, C; Oliveira, S. C; Meyer, R; Miyoshi, A; Azevedo, V.
Título: Construction and partial characterization of a Corynebacterium pseudotuberculosis bacterial artificial chromosome library through genomic survey sequencing
Fonte: Genet. mol. res. (Online);5(4):653-663, 2006. tab, ilus, graf.
Idioma: en.
Projeto: Brasil. Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Brasil. CAPES; . FINEP; . Fundação de Amparo à Pesquisa do Estado de Minas Gerais.
Resumo: Corynebacterium pseudotuberculosis is a gram-positive bacterium that causes caseous lymphadenitis in sheep and goats. However, despite the economic losses caused by caseous lymphadenitis, there is little information about the molecular mechanisms of pathogenesis of this bacterium. Genomic libraries constructed in bacterial artificial chromosome (BAC) vectors have become the method of choice for clone development in high-throughput genomic-sequencing projects. Large-insert DNA libraries are useful for isolation and characterization of important genomic regions and genes. In order to identify targets that might be useful for genome sequencing, we constructed a C. pseudotuberculosis BAC library in the vector pBeloBAC11. This library contains about 18,000 BAC clones, with inserts ranging in size from 25 to 120 kb, theoretically representing a 390-fold coverage of the C. pseudotuberculosis genome (estimated to be 2.5-3.1 Mb). Many genomic survey sequences (GSSs) with homology to C. diphtheriae, C. glutamicum, C. efficiens, and C. jeikeium proteins were observed within a sample of 215 sequenced clones, confirming their close phylogenetic relationship. Computer analyses of GSSs did not detect chimeric, deleted, or rearranged BAC clones, showing that this library has low redundancy. This GSSs collection is now available for further genetic and physical analysis of the C. pseudotuberculosis genome. The GSS strategy that we used to develop our library proved to be efficient for the identification of genes and will be an important tool for mapping, assembly, comparative, and functional genomic studies in a C. pseudotuberculosis genome sequencing project that will begin this year.
Descritores: Corynebacterium pseudotuberculosis/genética
Cromossomos Artificiais Bacterianos/genética
Cromossomos Bacterianos/genética
Biblioteca Gênica
Genoma Bacteriano/genética
-Clonagem Molecular
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 10 LILACS  
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Id: lil-479685
Autor: Gimenes, F; Gouveia, F. de S; Fiorini, A; Fernandez, M. A.
Título: Intrinsic bent DNA sites in the chromosomal replication origin of Xylella fastidiosa 9a5c
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(4):295-304, Apr. 2008. ilus, graf.
Idioma: en.
Projeto: CNPq; . Academy of Science for the Developing World; . CAPES.
Resumo: The features of the nucleotide sequences in both replication and promoter regions have been investigated in many organisms. Intrinsically bent DNA sites associated with transcription have been described in several prokaryotic organisms. The aim of the present study was to investigate intrinsic bent DNA sites in the segment that holds the chromosomal replication origin, oriC, of Xylella fastidiosa 9a5c. Electrophoretic behavior analyses, as well as in silico analyses of both the 2-D projection and helical parameters, were performed. The chromosomal segment analyzed contains the initial sequence of the rpmH gene, an intergenic region, the dnaA gene, the oriC sequence, and the 5' partial sequence of the dnaN gene. The analysis revealed fragments with reduced electrophoretic mobility, which indicates the presence of curved DNA segments. The analysis of the helical parameter ENDS ratio revealed three bent DNA sites (b1, b2, and b3) located in the rpmH-dnaA intergenic region, the dnaA gene, and the oriC 5' end, respectively. The chromosomal segment of X. fastidiosa analyzed here is rich in phased AT tracts and in CAnT motifs. The 2-D projection indicated a segment whose structure was determined by the cumulative effect of all bent DNA sites. Further, the in silico analysis of the three different bacterial oriC sequences indicated similar negative roll and twist >34.00° values. The DnaA box sequences, and other motifs in them, may be associated with the intrinsic DNA curvature.
Descritores: Cromossomos Bacterianos/genética
DNA Bacteriano/genética
Origem de Replicação/genética
Xylella/genética
-Sequência de Bases
Replicação do DNA/genética
Eletroforese em Gel de Ágar
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 10 LILACS  
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Bastos, Maria do Carmo de Freire
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Id: lil-470358
Autor: Balassiano, Ilana Teruszkin; Bastos, Maria do Carmo de Freire; Madureira, Danielle Jannuzzi; Silva, Iris Gripp da; Freitas-Almeida, Ângela Corrêa de; Oliveira, Selma Soares de.
Título: The involvement of tetA and tetE tetracycline resistance genes in plasmid and chromosomal resistance of Aeromonas in Brazilian strains
Fonte: Mem. Inst. Oswaldo Cruz;102(7):861-866, Nov. 2007. ilus, tab.
Idioma: en.
Resumo: This study analyzed the involvement of tetA and tetE genes in the tetracycline resistance of 16 strains of genus Aeromonas, isolated from clinical and food sources. Polymerase chain reactions revealed that 37.5 percent of the samples were positive for tetA, and also 37.5 percent were tetE positive. One isolate was positive for both genes. Only the isolate A. caviae 5.2 had its resistance associated to the presence of a plasmid, pSS2. The molecular characterization of pSS2 involved the construction of its restriction map and the determination of its size. The digestion of pSS2 with HindIII originated two fragments (A and B) that were cloned separately into the pUC18 vector. The tetA gene was shown to be located on the HindIII-A fragment by PCR. After transforming a tetracycline-sensitive strain with pSS2, the transformants expressed the resistance phenotype and harbored a plasmid whose size was identical to that of pSS2. The results confirmed the association between pSS2 and the tetracycline resistance phenotype, and suggest a feasible dissemination of tetA and tetE among strains of Aeromonas. This study suggests the spreading tetA and tetE genes in Aeromonas in Brazil and describes a resistance plasmid that probably contributes to the dissemination of the resistance.
Descritores: Aeromonas/efeitos dos fármacos
Antibacterianos/farmacologia
Antiporters/genética
Proteínas de Bactérias/genética
Alface/microbiologia
Resistência a Tetraciclina/genética
Tetraciclina/farmacologia
-Aeromonas/genética
Aeromonas/isolamento & purificação
Cromossomos Bacterianos/genética
Testes de Sensibilidade Microbiana
Fenótipo
Reação em Cadeia da Polimerase
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 10 LILACS  
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Id: lil-431595
Autor: Fernández, Sandra; Alonso, Guillermina; Toro, Elsa.
Título: Estructura de mosaico del cromosoma bacteriano: islas patogénicas / Mosaic of structure the bacterial chromosome: pathogenic islands
Fonte: Rev. Inst. Nac. Hig;35(2):20-31, 2004. tab, graf.
Idioma: es.
Resumo: Las islas patogénicas (PAIs) son segmentos de ADN bacteriano que portan uno o más genes de virulencia, los cuales han sido adquiridos en bloque de una fuente externa. El genoma de un patógeno usualmente representa un mosaico entre estas islas recién adquiridas y un ADN relativamente antiguo. Las PAIs son identificadas por su diferencia en el porcentaje de contenido de G+C en relación a la media del cromosoma y por otras características, que sugieren su adquisición a través de elementos genéticos móviles. Genes para una amplia gama de determinantes de virulencia están asociados con PAIs, incluyendo los que codifican para ciertos mecanismos que le permiten resistir las defensas del hospedador, factores de colonización, adquisición de nutrientes y toxinas. El estudio y conocimiento de las PAIs provee evidencias importantes de la biología de las bacterias patógenas. Los productos de las PAIs podrán representar, en un futuro, blancos para la terapia antimicrobiana, vacunas y herramientas diagnósticas
Descritores: Bactérias
Cromossomos Bacterianos
Genoma Bacteriano
Virulência
-Bacteriologia
Venezuela
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  6 / 10 LILACS  
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Id: lil-396382
Autor: Patiño Uzcátegui, Anelvi; Colmenares, Melisa; Díaz, LE; Grassi, H; Andrades, E.
Título: Estandarización del método para medir el efecto diferencial de los antibióticos antraciclínicos (ATC) doxorubicina y 4'epi doxorubicina sobre plásmido y/o cromosoma bacteriano in vivo / Standarized of the method for to measure the effect diferential of the anthacycline antibiotics (ATC) doxorubinic or 4'epi doxorubicin above plasmic and cromosome bacteriane in vitro
Fonte: Rev. Fac. Farm. (Merida);45(1):49-53, ene.-jun. 2003. tab, graf.
Idioma: es.
Resumo: Los antibióticos antraciclínicos (ATC) se utilizan en la terapéutica como antineoplásicos ya que actúan intercalándose entre las bases apareadas del ADN, modificando su estructura y conformación y afectando a las polimerasas y topoisomerasas. En el presente trabajo se intenta demostrar que la resistencia a antibióticos, cuando ésta se encuentra modificada por un plásmido, puede ser alterada por la presencia de ATC. La cepa bacteriana DH5 alfa/pUC19 se cultivo en medio tripticasa soya y luego se colocó en ausencia y presencia de ampicilina (como presión selectiva) y de una ATC (Doxorubicina a 4'epi Doxorubicina). El efecto se puso en evidencia por cuantificación del crecimiento y por evaluación del color en placas con X-gal. Los resultados sugieren que la Doxorubicina tiene un efecto negativo sobre la replicación del plásmido pero afecta positivamente la expresión del mismo. Mientras que 4'-epi Doxorubicina afecta negativamente ambos procesos. Se propone la 4'-epi Doxorubicina como antibiótico para curar plásmidos y así resistencias codificadas en plásmidos, haciéndose la bacteria sensible al antibiótico
Descritores: Antibióticos Antineoplásicos/análise
Antibióticos Antineoplásicos/uso terapêutico
Cromossomos Bacterianos
Plasmídeos/análise
Plasmídeos/farmacologia
-Farmacologia
Venezuela
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  7 / 10 LILACS  
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Mamizuka, E. M
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Id: lil-351151
Autor: Trindade, P. A; Mcculloch, J. A; Oliveira, G. A; Mamizuka, E. M.
Título: Molecular techniques for MRSA typing: current issues and perspectives
Fonte: Braz. j. infect. dis;7(1):32-43, Feb. 2003.
Idioma: en.
Resumo: Staphylococcus aureus has long been recognised as an important pathogen in human disease. Serious staphylococcal infections can frequently occur in inpatients and may lead to dire consequences, especially as to therapy with antimicrobial agents. The increase in the frequency of Methicillin-Resistant Staphylococcus aureus (MRSA) as the causal agent of nosocomial infection and the possibility of emergence of resistance to vancomycin demands a quick and trustworthy characterization of isolates and identification of clonal spread within hospitals. Enough information must be generated to permit the implementation of appropriate measures for control of infection, so that outbreaks can be contained. Molecular typing techniques reviewed in this manuscript include: plasmid profile analysis, analysis of chromosomal DNA after enzymatic restriction, Southern blotting, pulsed field gel electrophoresis (PFGE), techniques involving polymerase chain reaction and multilocus sequence typing (MLST). Repetitive DNA Sequence PCR (rep-PCR) may be used for screening due to its practicality, low cost and reproducibility. Because of its high discriminatory power Pulsed-Field Gel Electrophoresis (PFGE) still remains the gold standard for MRSA typing. New techniques with higher reproducibility and discriminatory power, such as Multi-Locus Sequence Typing (MLST), are appearing. These are mostly useful for global epidemiology studies. Molecular typing techniques are invaluable tools for the assessment of putative MRSA outbreaks and so should be extensively used for this purpose
Descritores: Técnicas de Tipagem Bacteriana
Infecção Hospitalar/microbiologia
DNA Bacteriano/análise
Resistência a Meticilina/genética
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/classificação
-Técnicas de Tipagem Bacteriana/métodos
Técnicas de Tipagem Bacteriana/normas
Brasil/epidemiologia
Cromossomos Bacterianos/química
Infecção Hospitalar/epidemiologia
Infecção Hospitalar/prevenção & controle
Eletroforese em Gel de Campo Pulsado
Genótipo
Reação em Cadeia da Polimerase
Plasmídeos/análise
Sequências Repetitivas de Ácido Nucleico
Infecções Estafilocócicas/epidemiologia
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/genética
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
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Id: lil-335995
Autor: Di Genaro, M. Silva; Escudero, M. Esther; Maccioni, Mariana; Guzmán, Ana M. Stefanini de.
Título: Protective immunity induced by a Yersinia enterocolitica serovar 0: 8 cellular extract
Fonte: Biocell;20(3):235-241, Dec. 1996.
Idioma: en.
Resumo: The purpose of this study was to investigate the protective power of a cellular extract (CE) from Y. enterocolitica 0:8 grown in condition of expression of chromosomal antigens. Mice were immunized by s.c. route and challenged with: 0 LD50 (1 x 10(4) CFU/ml). Immunoblotting showed that CE-specific serum reacted with several CE antigens. Prominent bands, of molecular weights 60 and 35.5, were present in cytoplasmic and membrane fraction, respectively. The lipopolysaccharide (LPS) was detected in CE. These findings suggest that chromosomally-encoded antigens present in CE may induce protection against Y. enterocolitica infection. Both humoral and cellular immune response contribute to protection in mice.
Descritores: Antígenos de Bactérias/imunologia
Yersinia enterocolitica
-Transferência Adotiva
Anticorpos Antibacterianos/biossíntese
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/administração & dosagem
Antígenos de Bactérias/genética
Cromossomos Bacterianos
Imunidade Celular
Imunização
Injeções Intraperitoneais
Injeções Subcutâneas
Lipopolissacarídeos/imunologia
Organismos Livres de Patógenos Específicos
Yersinia enterocolitica
Yersiniose
Limites: Animais
Masculino
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 10 LILACS  
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Id: lil-216822
Autor: Ruíz, Omar; Dagert, Manuel.
Título: Cloneo in vivo de genes de Escherichia coli K12 mediante recombinacion homologa / Cloning of gene of Escherichia coli K12 by means of homologue recombination
Fonte: Acta cient. venez;47(2):121-6, 1996. ilus, tab.
Idioma: es.
Projeto: CDCHT.
Resumo: Plasmid pT153, a stable recombinant between pBR322 plasmid and M13 bacteriophage, and Tn10 transposon were employed for in vivo cloning of a chromosomal segment of Escherichia coli including the nar locus. The strategy consisted in creating an homology between pT153 and the E. coli chromosome, incorporating a Tn10 transposon close to the nar locus of a polA12 temperature-sensitive strain. The selection of clones carrying the plasmid into the chromosome was realized at 42 degrees C, with ampiciline, taken advantage that the replicon of pT153 requires the ADN Polymerase I for its functioning. The plasmid integrated in the polA12 strain has the opportunity of excising and carrying part of bacterial genome and autonomically replicating after a thermal change from 42 degrees C to 30 degrees C. The stable recombinant plasmids could be efficiently transduced with the M13 bacteriophage to a E. coli strain (delta narGHJI), obtaining Nit+ Apr transductant clones. The versatility of this method of E. coli gene cloning is the facility to create the homologue region anywhere in the bacterial genome and the efficient transduction of pT153 plasmid by M13 bacteriophage
Descritores: Cromossomos Bacterianos/genética
Clonagem Molecular
Clonagem de Organismos
Escherichia coli/genética
Recombinação Genética
-Plasmídeos/genética
Responsável: BR1.1 - BIREME


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Id: lil-165941
Autor: Barrera, L.
Título: Acerca de la resistencia de las micobacterias a los antibióticos: un enfoque microbiológico / About of antibiotic resistance to mycobacterial: an microbiological focus
Fonte: Infectol. microbiol. clin;6(6):182-9, 1994. ilus, tab.
Idioma: es.
Descritores: Antituberculosos/efeitos adversos
Resistência Microbiana a Medicamentos/genética
Infecções por Mycobacterium/tratamento farmacológico
Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico
Tuberculose/tratamento farmacológico
-Cromossomos Bacterianos
Infecções por Mycobacterium/complicações
Tuberculose Resistente a Múltiplos Medicamentos/genética
Tuberculose Resistente a Múltiplos Medicamentos/prevenção & controle
Limites: Humanos
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco



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