Base de dados : LILACS
Pesquisa : A11.284.187.360 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Azevedo, Joäo Lucio de
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Id: lil-531760
Autor: Giancoli, Ágata Cristiane Huppert; Azevedo, João Lúcio de; Kleiner, Aline Aparecida.
Título: Cytological characterization of an Aspergillus nidulans mutant from a strain with chromosomic duplication
Fonte: Braz. j. microbiol;41(1):264-269, Jan.-Mar. 2010. ilus.
Idioma: en.
Resumo: A development mutant, named V103, was obtained spontaneously from the A strain of A. nidulans. The A strain contains a duplicated segment of chromosome I that has undergone translocation to chromosome II (I ¨ II). It is mitotically unstable and generates phenotypically deteriorated types, some with enhanced stability. The deteriorated variants of A. nidulans show abnormal development, exhibiting slower colony growth, variations in colony pigmentation and changes in conidiophore structure. The alterations observed in the conidiophore include fewer metulae and phialides, further elongation and ramification of these structures, delayed nuclear migration and the presence of secondary conidiophores.
Descritores: Aspergillus nidulans/crescimento & desenvolvimento
Aspergillus nidulans/isolamento & purificação
Movimento Celular
Cromossomos Fúngicos
Esporos Fúngicos/genética
Fenótipo
Supressão Genética
-Métodos
Microscopia Eletrônica
Técnicas
Virulência
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-520042
Autor: Lucena, B. T. L; Silva-Filho, E. A; Coimbra, M. R. M; Morais, J. O. F; Simões, D. A.; Morais Junior, M. A.
Título: Chromosome instability in industrial strains of Saccharomyces cerevisiae batch cultivated under laboratory conditions
Fonte: Genet. mol. res. (Online);6(4):1072-1084, 2007. ilus.
Idioma: en.
Resumo: Industrial ethanol fermentation is a complex microbiological process to which yeast cells must adapt for survival. One of the mechanisms for adaptation is thought to involve chromosome rearrangements. We found that changes in chromosome banding patterns measured by pulsed-field gel electrophoresis can also be produced in laboratory media under simulated industrial conditions. Based on analysis of their generational variation, we found that these chromosome changes were specific to the genetic backgrounds of the initial strains. We conclude that chromosome rearrangements could be one of the factors involved in yeast cell adaptation to the industrial environment.
Descritores: Instabilidade Cromossômica
Cromossomos Fúngicos/genética
DNA Fúngico/genética
Etanol/metabolismo
Saccharomyces cerevisiae/genética
-Adaptação Fisiológica
Biotecnologia
Impressões Digitais de DNA
DNA Fúngico/isolamento & purificação
Fermentação
Cariotipagem
Reatores Biológicos/microbiologia
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/fisiologia
Responsável: BR26.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: lil-495752
Autor: Baeza, Marcelo E; Sanhueza, Mario A; Cifuentes, Víctor H.
Título: Occurrence of killer yeast strains in industrial and clinical yeast isolates
Fonte: Biol. Res;41(2):173-182, 2008. ilus, tab, graf.
Idioma: en.
Projeto: Universidad de Chile; . Fondecyt.
Resumo: The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsRNA) or in nuclear genes. The spectrum of action and the activity of killer toxins are influenced by temperature, salinity and pH of media. In the present work we determined the existence of K+ in a collection of S. cerevisiae and P. anómala yeasts isolated from environmental, industrial and clinical sources. The assays were performed in strains belonging to three yeast genera used as sensitive cells and under a wide range of pH and temperatures. Approximately 51 percent of isolates tested showed toxicity against at least one sensitive yeast strain under the conditions tested. The K+ P. anómala isolates showed a wide spectrum of action and two of them had toxic activity against strains of the three yeast genera assayed, including C. albicans strains. In all S. cerevisiae K+ isolates an extrachromosomal dsRNA molecule (4.2 Kb) was observed, contrary to P. anómala K+ isolates, which do not possess any EGEs. The K+ phenotype is produced by an exported protein factor and the kinetics of killer activity production was similar in all isolates with high activity in the log phase of growth, decaying in the stationary phase.
Descritores: Fatores Matadores de Levedura/biossíntese
Pichia/metabolismo
Saccharomyces cerevisiae/metabolismo
-Cromossomos Fúngicos/genética
DNA Fúngico/genética
DNA Mitocondrial/genética
Eletroforese em Gel de Ágar
Meio Ambiente
Concentração de Íons de Hidrogênio
Fenótipo
Reação em Cadeia da Polimerase
Pichia/genética
Saccharomyces cerevisiae/genética
Temperatura
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-280151
Autor: Rodrigues, E. C; Tanaka, Y; Pizzirani-Kleiner, A. A.
Título: Observaçöes citológicas em conídios e células miceliais de humicola sp / Cytological observations in conidial and mycelial cells of humicola sp
Fonte: Rev. microbiol;21(3):232-7, set. 1990. ilus.
Idioma: pt.
Resumo: Resumo: O objetivo desse estudo foi descrever aspectos do comportamento nuclear durante a divisäo miótica em conídios e células miceliais do fungo celulolítico Humicola so. A sequência dos eventos mitóticos foi similar aquela observada em outros fungos filamentosos. O número de cromossomos observados no final da metáfase näo pode ser determinado com segurança, mas pareceu estar entre 6 e 8. As culturas de Humicola sp frequentemente mostraram anastomose de hifas e a presença de núcleos na ponte hifal, o que permitiria a ocorrência de heterocariose,e indica que o ciclo parasexual pode ocorrer nessa espécie (au)
Descritores: Cromossomos Fúngicos/fisiologia
Divisão Celular/fisiologia
Fungos/citologia
Metáfase/fisiologia
Mitose/fisiologia
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL


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Id: lil-212283
Autor: Nobrega, M. P; Graminha, M. A. S; Troitskaya, E. N; Nobrega, F. G.
Título: Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;31(3):355-63, Mar. 1998. tab, graf.
Idioma: en.
Projeto: FAPESP; . FINEP; . CNPq.
Resumo: The mutants of Saccharomyces cerevisiae assigned to complementation group G199 are deficient in mitochondrial respiration and lack a functional cytochrome oxidase complex. Recombinant plasmids capable of restoring respiration were cloned by transformation of mutants of this group with a yeast genomic library. Sequencing indicated that a 2.1-kb subclone encompasses the very end (last 11 amino acids) of the PET111 gene, the COX7 gene and a new gene (YMR255W) of unknown function that potentially codes for a polypeptide of 188 amino acids (about 21.5 kDa) without significant homology to any known protein. We have shown that the respiratory defect corresponding to group G199 is complemented by plasmids carrying only the COX7 gene. The gene YMR255W was inactivated by one-step gene replacement and the disrupted strain was viable and unaffected in its ability to grow in a variety of different test media such as minimal or complete media using eight distinct carbon sources at three pH values and temperatures. Inactivation of this gene also did not affect mating or sporulation.
Descritores: Cromossomos Fúngicos/genética
DNA Fúngico/genética
Saccharomyces cerevisiae/genética
-Sequência de Aminoácidos/genética
Sequência de Bases/genética
Clonagem Molecular
Complexo IV da Cadeia de Transporte de Elétrons/genética
Genótipo
Mutação/genética
Fenótipo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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