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Pesquisa : A11.284.187.560 [Categoria DeCS]
Referências encontradas : 31 [refinar]
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  1 / 31 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-886840
Autor: CORDEIRO, JOEL M P; KAEHLER, MIRIAM; SOUZA, GUSTAVO; FELIX, LEONARDO P.
Título: Karyotype analysis in Bignonieae (Bignoniaceae): chromosome numbers and heterochromatin
Fonte: An. acad. bras. ciênc;89(4):2697-2706, Oct.-Dec. 2017. graf.
Idioma: en.
Resumo: ABSTRACT Chromosome numbers and heterochromatin banding pattern variability have been shown to be useful for taxonomic and evolutionary studies of different plant taxa. Bignonieae is the largest tribe of Bignoniaceae, composed mostly by woody climber species whose taxonomies are quite complicated. We reviewed and added new data concerning chromosome numbers in Bignonieae and performed the first analyses of heterochromatin banding patterns in that tribe based on the fluorochromes chromomycin A3 (CMA) and 4'-6-diamidino-2-phenylindole (DAPI). We confirmed the predominant diploid number 2n = 40, as well as variations reported in the literature (dysploidy in Mansoa [2n = 38] and polyploidy in Dolichandra ungis-cati [2n = 80] and Pyrostegia venusta [2n = 80]). We also found a new cytotype for the genus Anemopaegma (Anemopaegma citrinum, 2n = 60) and provide the first chromosome counts for five species (Adenocalymma divaricatum, Amphilophium scabriusculum, Fridericia limae, F. subverticillata, and Xylophragma myrianthum). Heterochromatin analyses revealed only GC-rich regions, with six different arrangements of those bands. The A-type (one large and distal telomeric band) were the most common, although the presence and combinations of the other types appear to be the most promising for taxonomic studies.
Descritores: Heterocromatina/genética
Bignoniaceae/genética
Cromossomos de Plantas
Cariótipo
-Ploidias
Bignoniaceae/classificação
Responsável: BR1.1 - BIREME


  2 / 31 LILACS  
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Id: lil-641006
Autor: Felismino, M. F; Silva, N; Pagliarini, M. S; Valle, C. B.
Título: Mitotic behavior in root tips of Brachiaria genotypes with meiotic chromosome elimination during microsporogenesis
Fonte: Genet. mol. res. (Online);7(2):336-341, 2008. tab, ilus.
Idioma: en.
Resumo: Three accessions of Brachiaria brizantha, three of B. humidicola, and two interspecific hybrids between B. ruziziensis and B. brizantha were analyzed with regard to their mitotic behavior in root tips. All these genotypes revealed chromosome elimination or lack of chromosome affinity in previous analyses of microsporogenesis. Analyses of root tips showed a normal mitotic division in all accessions and hybrids, reinforcing the notion that the genetic control of meiosis is totally independent of that of mitosis. The implications of these findings for the Brachiaria breeding program are discussed.
Descritores: Brachiaria/genética
Cromossomos de Plantas/genética
-Brachiaria/citologia
Genótipo
Meiose/genética
Pólen/citologia
Pólen/genética
Responsável: BR26.1 - Biblioteca Central


  3 / 31 LILACS  
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Id: lil-640999
Autor: Peñaloza, A. P. S; Côrtes, A. L; Pozzobon, M. T; Santos, S; Rua, G. H; Valls, J. F. M.
Título: Unusual chromosome numbers in Paspalum L. (Poaceae: Paniceae) from Brazil
Fonte: Genet. mol. res. (Online);7(2):399-406, 2008. tab, ilus.
Idioma: en.
Resumo: Somatic chromosome numbers were determined for 20 new germplasm accessions of Paspalum, belonging to 17 species collected in Brazil. Chromosome number is reported for the first time for P. reduncum (2n = 18), P. cinerascens (2n = 20), P. cordatum (2n = 20), P. filgueirasii (2n = 24), P. ammodes (2n = 36), P. bicilium (2n = 40), P. heterotrichon (2n = 40), and P. burmanii (2n = 48). New cytotypes were confirmed for two germplasm accessions of P. carinatum (2n = 30) and P. trachycoleon (2n = 36), one of P. clavuliferum (2n = 40) and one of P. lanciflorum (2n = 40), indicating variability in these species. The remaining chromosome numbers reported here confirm previous counts. The unexpected chromosome numbers 2n = 18, 24, 36, and 48 in Paspalum species, which are usually shown to be multiples of 10, suggest that much more collection and cytogenetic characterization are necessary to assess the whole chromosomal and genomic multiplicity present in the genus, which seems to be much more diverse than currently thought to be.
Descritores: Cromossomos de Plantas/genética
Paspalum/genética
-Brasil
Análise Citogenética
Filogenia
Poliploidia
Paspalum/classificação
Responsável: BR26.1 - Biblioteca Central


  4 / 31 LILACS  
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Id: lil-640996
Autor: Fuzinatto, V. A; Pagliarini, M. S; Valle, C. B.
Título: Evaluation of microsporogenesis in an interspecific Brachiaria hybrid (Poaceae) collected in distinct years
Fonte: Genet. mol. res. (Online);7(2):424-432, 2008. tab, ilus.
Idioma: en.
Resumo: Microsporogenesis in an interspecific Brachiaria hybrid, grown in the field under natural environmental conditions in Brazilian savannas, was analyzed in three distinct years of collection. Several types of meiotic abnormalities were recorded during those three years, but varied in type and frequency depending on the year. The average temperature and rainfall 15 days before collection was unusually high in those years. The percentage of abnormal meiocytes recorded was 62% in 2001, 73% in 2004, and 77% in 2005. The abnormalities observed during microsporogenesis compromised pollen viability by generating unbalanced gametes or affecting nucleolus organization. The environmental conditions under which the hybrid was growing could have affected the genetic control of meiosis. More detailed studies, under controlled conditions, are necessary to better understand the effects of environmental factors on Brachiaria microsporogenesis hybrids.
Descritores: Brachiaria/genética
Meiose/genética
-Brachiaria/citologia
Segregação de Cromossomos
Cromossomos de Plantas/genética
Hibridização Genética
Temperatura
Responsável: BR26.1 - Biblioteca Central


  5 / 31 LILACS  
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Texto completo SciELO Costa Rica
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Id: lil-637628
Autor: Imery-Buiza, José.
Título: Inestabilidad cariológica durante la formación de células madres del polen en Aloe vera (Aloaceae)
Fonte: Rev. biol. trop;55(3/4):805-813, Sep.-Dec. 2007. ilus, tab.
Idioma: es.
Resumo: Con el objetivo de esclarecer la posible existencia de anomalías citogenéticas que aminoren la fertilidad del polen de Aloe vera, se analizó la etapa de proliferación celular que lleva a la formación de células madres del polen (CMPs). Se recolectaron botones florales (BF) en 25 plantas de una población ubicada a 10°34'15'' N, 64°12'08'' W, los cuales fueron fijados en Carnoy I por 24 h y almacenados en etanol (70 % v/v). Las observaciones se realizaron en preparaciones temporales obtenidas por la tinción del contenido de las anteras suspendidas en orceína acética (1.5 % p/v) por 5 minutos. De las 9 411 células analizadas, 17 % mostraron 1-8 puentes entre cromátidas hermanas, 13 % 1-7 micronúcleos de 0.9-4.8 µm, 8.1 % estaban unidas por puentes y 0.1 % no contenían cromatina. El resto de las células (61.8 %) presentó configuraciones aparentemente normales y sin variaciones morfométricas. La proliferación irregular de una fracción de CMPs (39.2 %) sugiere que las condiciones ambientales de la zona árida donde se realizaron los muestreos inducen inestabilidad cromosómica y cambios fisiológicos que afectan el normal desarrollo de la mitosis premeiótica, generando pérdida o adición de fragmentos, asociados a deficiencias y duplicaciones génicas.
Descritores: Aloe/citologia
Instabilidade Cromossômica/genética
Cromossomos de Plantas/genética
Mitose/genética
Pólen/citologia
Células-Tronco/citologia
-Aloe/genética
Pólen/genética
Venezuela
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


  6 / 31 LILACS  
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Texto completo SciELO Costa Rica
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Id: lil-637594
Autor: Sahoo, Pragnya; Jena, Satyanarayan; Mohanty, Suprava; Bandhu Das, Anath.
Título: Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers
Fonte: Rev. biol. trop;55(2):437-448, jun. 2007. ilus, tab.
Idioma: en.
Resumo: Analysis of karyotype, nuclear DNA content and RAPD markers were performed in four species of Bruguiera (Rhizophoraceae) of Bhitarkanika mangrove forests, Orissa, India. Detailed karyotype analysis revealing 2n=34 in B. cylindrica and 2n=36 in B. gymnorrhiza was reported for the first time and 2n=34 in B. parviflora and B. sexangula was confirmed. On the basis of the common types of chromosomes present among Bruguiera, two distinct groups were found; one consists of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula. The symmetrical karyotype with same chromosome types grouped B. cylindrica and B. parviflora together and presence of Type E chromosomes placed B. gymnorrhiza and B. sexangula in a separate group, suggesting their closer affinity in their respective group. Analysis of chromosome length, volume, INV and 4C DNA content confirmed this division. Nuclear DNA content was two-fold higher (~17.0 pg) in the second group than in the first (~8.0 pg). The amplification products generated through RAPD revealed 1-9 amplicons with size variations from 600 bp to 2 500 bp with 49.31% genetic similarity between B. gymnorrhiza and B. sexangula and 47.10% in between B. cylindrica and B. parviflora. The high copy number marker band (~ 1 100 bp) yielded in OPN-15 primer in B. parviflora the characteristic DNA marker, which was cloned and used as probes for assessment of genetic diversity, and demonstrated its close genetic affinity to B. cylindrica. B. gymnorrhiza and B. sexangula also produced similar marker bands of ~600 bp and ~2 200 bp in the same primer. All of the cytological, 4C DNA content and RAPD data confirmed the existence of two taxonomically distinct groups of Bruguiera: one consisting of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula as placed earlier (1862) in the tribe Rhizophoreae by Bentham and Hooker, on the basis of the flowering habits of Bruguiera. Genetically, the B. sexangula and B. gymnorrhiza group was found to be very closely, rather than distantly, related to B. parviflora and B. cylindrica. Our results demonstrate that molecular markers together with cytological evidence provide an effective tool to access the existing interspecific genetic polymorphism in mangrove species, to solve the taxonomic problems and to design their conservation strategy. Rev. Biol. Trop. 55 (2): 437-448. Epub 2007 June, 29.

Estudiamos cuatro especies del mangle Bruguiera (Rhizophoraceae) en Orissa, India. Los cromosomas indican queB. cylindrica y B. parviflora son un grupo taxonómico, y que B. gymnorrhiza y B. sexangula son otro. Genéticamente, el par B. sexangula y B. gymnorrhiza está cercanamente emparentado con B. parviflora and B. cylindrica. Nuestros datos indican que el uso combinado de marcadores genéticos y evidencia citológica permiten discernir el polimorfismo genético interespecífico en los mangles, tanto para resolver problemas taxonómicos como para diseña estrategias eficaces de conservación.
Descritores: Cromossomos de Plantas/genética
DNA de Plantas/análise
Filogenia
Técnica de Amplificação ao Acaso de DNA Polimórfico
Rhizophoraceae/genética
-Núcleo Celular/genética
Marcadores Genéticos
Cariotipagem
Rhizophoraceae/classificação
Especificidade da Espécie
Árvores/classificação
Árvores/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


  7 / 31 LILACS  
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Id: lil-633236
Autor: Sayuri Utsunomiya, Karina; Suely Paglirini, Maria; Borges do Valle, Cacilda.
Título: Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho & Hiern) Stapf (Gramineae)
Fonte: Biocell;29(3):295-301, Aug.-Dec. 2005. ilus, tab.
Idioma: en.
Resumo: The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho & Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x = 36). Chromosomes paired preferentially as bivalents, but quadrivalents were found in high frequencies in some cells. Meiotic behavior was, in general, irregular, and varied among accessions. Most accessions presented more than 20% of abnormal tetrads. The most common meiotic abnormalities were those related to irregular chromosome segregation due to polyploidy, leading to micronuclei formation in the tetrad stage. A low frequency of other meiotic abnormalities such as the absence of cytokinesis, chromosome stickiness, cell fusion, anaphase bridges, and chromosome transfer among microsporocytes were also recorded in some accessions. Limitations of these accessions for use in hybridization programs are discussed.
Descritores: Brachiaria/genética
Brachiaria/fisiologia
Poliploidia
-Brachiaria/citologia
Aberrações Cromossômicas
Cromossomos de Plantas/genética
Meiose
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


  8 / 31 LILACS  
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Id: lil-621400
Autor: Grossi, Juliano Almeida; Godoy, Sara Mataroli de; Macedo, Camila Ronchi; Paula, Gabriela Barbosa Navarro de; Romagnolo, Mariza Barion; Risso-Pascotto, Claudicéia.
Título: Comportamento meiótico durante a microsporogênese em espécies da família meliaceae / Comportamiento meiótica durante la microsporogénesis en especies de la familia meliaceae / Meiotic behavior during microsporogenesis of some species of meliaceae family
Fonte: Arq. ciênc. vet. zool. UNIPAR;14(1), jan.-jun. 2011. ilus, tab.
Idioma: pt.
Resumo: A família Meliaceae inclui espécies de grande interesse agronômico, ecológico, econômico e ornamental de alto potencial madeireiro no mundo. No Brasil ocorrem cerca de seis gêneros e 100 espécies. Considerando a falta de estudos citológicos para esse grupo, este trabalho descreve o comportamento meiótico durante a microsporogênese de seis espécies (Trichilia pallida, Trichilia elegans, Trichilia catigua, Cedrela fissilis, Cabralea canjerana, Guarea guidonea) da família Meliaceae, representando quatro dos seis gêneros que estão presentes no Brasil. Inflorescências foram coletadas e fixadas em etanol/ácido acético (3:1 v/v) por 24 horas, transferidos para álcool a 70%, e acondicionadas sob refrigeração. As lâminas foram preparadas pela técnica de esmagamento, coradas com carmim acético a 1%. A análise citogenética revelou poucas irregularidades meióticas, sendo estas relacionadas a segregação dos cromossomos, fusos irregulares e conexão citoplasmática. Como consequência da segregação irregular dos cromossomos na meiose I e II e da organização irregular dos fusos na meiose II, o padrão de citocinese também foi irregular originando tétrades com micrócitos e tríades, resultando em micrósporos desbalanceados e de núcleo restituído (2n).

The Meliaceae family includes the most important species with great agronomic, ecological, economic and ornamental value and high timber potential in the world. In Brazil, approximately six genera and 100 species are found. Considering the lack of cytological studies for its genera, the present study describes the meiotic behavior during microsporogenesis of six species of the Meliaceae family, representing four of the six genera that are present in Brazil; these species are: Trichilia pallida, Trichilia elegans, Trichilia catigua, Cedrela fissilis, Cabralea canjerana, Guarea guidonea. Inflorescences were collected and fixed in ethanol/acetic acid (3:1 v/v) for 24 hours, transferred to alcohol at 70%, and stored under refrigeration. The slides were prepared by squashing, and stained with acetic carmine 1% and observed under optical microscopy. Cytogenetic analysis revealed few meiotic irregularities, which are related to segregation of chromosome spindles and irregular cytoplasmic connection. As a result of irregular segregation of chromosomes during meiosis I and II and the irregular organization of the spindles in meiosis II, the pattern of cytokinesis was also irregular resulting in microcytic tetrads and triads, unbalanced microspores and restored nuclei (2n).

La familia Meliaceae incluye especies de gran interés agronómico, ecológico, económico y ornamental de alto potencial maderero en el mundo. En Brasil ocurren cerca de seis géneros y 100 especies. Considerando la falta de estudios citológicos para ese grupo, este trabajo describe el comportamiento meiótica durante la microsporogénesis de seis especies (Trichilia pallida, Trichilia elegans, Trichilia catigua, Cedrela fissilis, Cabralea canjerana, Guarea guidonea) de la familia Meliaceae, representando cuatro de los seis géneros que están presentes en Brasil. Inflorescencias fueran colectadas y fijadas en etanol/ácido acético (3:1 v/v) por 24 horas, transferidos para alcohol a 70%, y acondicionadas bajo refrigeración. Las láminas fueron preparadas por la técnica de aplastamiento, coloradas con carmín acético a 1%. El análisis citogenética reveló pocas irregularidades meióticas, siendo éstas relacionadas a la segregación de los cromosomas, fusos irregulares y conexión citoplasmática. Como consecuencia de segregación irregular de cromosomas en la meiosis I y II y de la organización irregular de los fusos en la meiosis II, el estándar de citocinesis también fue irregular originando tétradas con micrófitos y tríades, resultando en micros poros desbalanceados y de núcleo restituido (2n).
Descritores: Esporos/crescimento & desenvolvimento
Gametogênese Vegetal/genética
Meiose/genética
Meliaceae/crescimento & desenvolvimento
-Cromossomos de Plantas
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  9 / 31 LILACS  
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Id: lil-595006
Autor: Talia, Paola; Greizerstein, Eduardo J; Hopp, H. Esteban; Paniego, Norma; Poggio, Lidia; Heinz, Ruth A.
Título: Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
Fonte: Biocell;35(1):19-28, Apr. 2011. ilus, tab.
Idioma: en.
Resumo: Bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect C-PRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.
Descritores: Análise de Sequência de DNA/métodos
Cromossomos de Plantas
Cromossomos Artificiais Bacterianos/genética
Helianthus/genética
Hibridização in Situ Fluorescente/métodos
-Sequência de Bases
Marcadores Genéticos
Locos de Características Quantitativas
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  10 / 31 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-567803
Autor: Rainho, Claudia R. de; Kaezer, Andréa; Aiub, Claudia A. F; Felzenszwalb, Israel.
Título: Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation
Fonte: An. acad. bras. ciênc;82(4):925-932, Dec. 2010. ilus, tab.
Idioma: en.
Resumo: N-nitroso compounds, such as N-nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH) using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM) genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water) and positive control maleic hydrazide (MH 30mg/mL). After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI) and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM), showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dose-dependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.

Nitrocompostos, como N-nitrosodietilamina (NDEA), podem ser formados pela reação entre uma amina secundária e agentes nitrosantes e são suspeitos de estarem envolvidos na formação de tumores em humanos. NDEA é considerada um carcinógeno fraco e ensaios genotóxicos provavelente pela utilização de um sistema de ativação ineficiente e/ou pela utilização de um eficiente sistema de reparo. Neste trabalho, nós avaliamos a sensibilidade de ensaios com Alliu cepa L. e Tradescantia pallida var. purpurea (Trad-SH) à genotoxicidade e mutagenicidade induzidas por diferentes concentrações de NDEA (0,1; 0,5; 5 e 25mM) por 3h, por 3 dias consecutivos, incluindo controle negativo (água destilada) e controle positivo, hidrazida maleica (MH 30mg/mL). Depois do tratamento, as raízes foram hidrolizadas, esmagadas e o índice mitótico (IM) e anormalidades citológicas foram contadas. Os resultados revelaram um efeito citostático de NDEA (0,5 e 5mM), pela significante redução do IM. Chromosome stickiness sugere um efeito citotóxico de NDEA. T pallida purpurea não respondeu ao mutágeno com um padrão dose dependente. Em conclusão, nossos estudos indicaram que raízes de Allium cepa L. possue sensibilidade na detecção genotóxica de NDEA, mas não para o ensaio Trad-SH.
Descritores: Aberrações Cromossômicas/induzido quimicamente
Dietilnitrosamina/toxicidade
Cebolas/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Tradescantia/efeitos dos fármacos
-Cromossomos de Plantas/efeitos dos fármacos
Cromossomos de Plantas/genética
Testes de Mutagenicidade
Cebolas/genética
Raízes de Plantas/genética
Tradescantia/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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