Base de dados : LILACS
Pesquisa : A11.284.430.106.279.692.630 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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Texto completo SciELO Chile
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Id: lil-772306
Autor: Cornejo, R; Garrido, O; Jaramillo, R; Vergara, E; Lermanda, P; Gatica, Y.
Título: Poros Nucleares y Función Celular en Epitelio Mamario / Nuclear Pores and Cellular Function
Fonte: Int. j. morphol;33(4):1269-1272, Dec. 2015. ilus.
Idioma: es.
Resumo: El presente artículo tiene como objetivo central evidenciar la interesante relación que se establece entre la función celular y el número de poros nucleares, relación que modula el activo intercambio nucleo-citoplasmatico en distintas etapas del ciclo celular de la estirpe HC11.

The main objective of this article is related to the study of different existing relationships between cellular function and the number of nuclear pores in order to explain the amount of nuclear-cytoplasmatic exchange through HC11 cell cycle stages.
Descritores: Glândulas Mamárias Animais/citologia
Glândulas Mamárias Animais/ultraestrutura
Poro Nuclear/ultraestrutura
-Diferenciação Celular
Células Epiteliais/ultraestrutura
Microscopia Eletrônica de Transmissão
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-718194
Autor: Souza, Romeu R. de; Oliveira, Vanessa C. de; Curi, Tania Cristina P.; Maldonado, Diogo C..
Título: Effects of ovariectomy on the secretory apparatus in the right atrial cardiomyocytes of middle-aged mice
Fonte: Clinics;69(8):554-558, 8/2014. tab, graf.
Idioma: en.
Resumo: OBJECTIVE : The aim of the present study was to evaluate the effects of ovariectomy on the secretory apparatus of natriuretic peptides in right atrial cardiomyocytes. METHODS: Nine-month-old mice underwent bilateral ovariectomy or sham surgery. The blood exam of the ovariectomized mice showed results consistent with castrated females. Systolic blood pressure was measured after ovariectomy (9 mo of age) and at the moment of sacrifice (12 mo of age). Fragments of the right atrium were collected and prepared for electron microscopy examination. The following variables were quantified: the quantitative density and area of the natriuretic peptide granules, the relative volume of euchromatin in the nucleus, the number of pores per 10 μm of the nuclear membrane and the relative volumes of the mitochondria and Golgi complex. RESULTS: The cardiomyocytes obtained from ovariectomized mice indicated that the quantitative density and the area of secretory granules of natriuretic peptides were significantly lower compared with the sham-operated mice. Furthermore, there was a decrease in the relative volume of euchromatin, a lower density of nuclear pores, and lower relative volumes of the mitochondria and Golgi complex in the ovariectomized mice compared with the sham-operated mice. These findings suggest a pool with a low turnover rate, i.e., low synthesis and elimination of natriuretic peptides. CONCLUSION: A lack of estrogen caused hypotrophy of the secretory apparatus in right atrial cardiomyocytes that could explain the weak synthesis of natriuretic peptides in mice. Furthermore, one of the mechanisms of blood pressure control was lost, which may explain, in part, the elevated blood pressure in ovariectomized mice. .
Descritores: Fator Natriurético Atrial/efeitos dos fármacos
Miócitos Cardíacos/ultraestrutura
Ovariectomia/efeitos adversos
-Fator Natriurético Atrial/análise
Pressão Sanguínea
Estradiol/sangue
Estrogênios/fisiologia
Eucromatina/ultraestrutura
Complexo de Golgi/ultraestrutura
Átrios do Coração/citologia
Tamanho Mitocondrial
Modelos Animais
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Poro Nuclear/ultraestrutura
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-595042
Autor: Cavicchia, Juan Carlos; Guembe, Gustavo; Fóscolo, Mabel.
Título: Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: a freeze-fracture study
Fonte: Biocell;34(2):81-89, Aug. 2010. ilus, graf.
Idioma: en.
Resumo: In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free areas, which are characteristic of other apoptotic models. Electron microscopy of thin-sections shows, coincidently with findings in the freeze-fracture replicas, a moderately irregular aggregation of marginal heterochromatin condensations. After nuclear fragmentation and micronuclear formation, pores behave in the usual manner in other apoptotic models, i.e., mainly showing migrations of nuclear pores toward the chromatin-free areas. The present results support the hypothesis that nuclear pore complexes are dynamic structures, which permit their migration toward nuclear membrane areas devoid of chromatin aggregations that might block the nucleocytoplasmic transport in such areas.
Descritores: Corpo Lúteo/citologia
Poro Nuclear/ultraestrutura
Técnica de Fratura por Congelamento/métodos
-Marcação In Situ das Extremidades Cortadas
Parto
Prenhez
Ratos Wistar
Limites: Masculino
Animais
Feminino
Gravidez
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


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Id: lil-449013
Autor: Arcavi, M; Orfus, G.
Título: Prevalencia de anticuerpos anti envoltura nuclear y sus isotipos en sueros positivos para anticuerpos antinucleares / Prevalence of antinuclear envelope antibodies and their isotypes in sera positive for antinuclear antibodies
Fonte: Medicina (B.Aires);66(4):327-331, 2006. tab, ilus.
Idioma: es.
Resumo: Antinuclear antibodies detected in HEp-2 cells by indirect immunofluorescence assay display a great variety of images, including the nuclear envelope pattern. This is quite a less frequent finding. Two thousand five hundred and ninety-four sera were processed, and 37.6% of ANA were detected. The prevalence of anti-nuclear envelope antibodies (ANEA) was of 1.2%, with a high association with autoimmune liver diseases (83%) and a low association with systemic lupus erythematosus. In 21 sera of patients with ANEA, no anti-DNAn antibodies were found; but 28.6% of anti-smooth muscle antibodies and 19% of anti-mitochondrial antibodies were detected. The triple rodent tissue section proved to be a less sensitive substrate than HEp-2 for the detection of ANEA. When using conjugates against different isotypes of antibodies for the detection of ANA, 90.5% of IgG, 66.6% of IgA and 9.5% of IgM. Two patients had ANEA-IgA at high titers (> or = 1:160) without ANEA-IgG. In this work, the importance of performing complementary tests for the detection of anti-smooth muscle antibodies, anti-mitochondrial antibodies and anti-DNAn is highlighted in order to apply these tests as guidelines for the clinical diagnosis of patients with ANEA. Besides, this study expresses the need of using total anti-Ig antibodies as conjugate for IIF-HEp-2 instead of anti-lgG; until the role of IgA antibodies in these autoimmune diseases is clarified.
Descritores: Anticorpos Antinucleares/sangue
Isotipos de Imunoglobulinas
Membrana Nuclear
-Anticorpos Antinucleares/imunologia
Células Epiteliais/imunologia
Técnica Indireta de Fluorescência para Anticorpo
Isotipos de Imunoglobulinas
Lâmina Nuclear/imunologia
Modelos Animais
Poro Nuclear/imunologia
Limites: Animais
Ratos
Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-445596
Autor: Becak, Maria Luiza; Fukuda-Pizzocaro, Kazumi.
Título: Pore-linked filaments in anura spermatocyte nuclei
Fonte: An. acad. bras. ciênc;79(1):63-70, Mar. 2007. ilus.
Idioma: en.
Projeto: Fundacao de Amparo à Pesquisa do Estado de Sao Paulo.
Resumo: Pore-linked filaments were visualized in spreads of anuran spermatocyte nuclei using transmission electron microscope. We used Odontophrynus diplo and tetraploid species having the tetraploid frogs reduced metabolic activities. The filaments with 20-40 nm width are connected to a ring component of the nuclear pore complex with 90-120 nm and extend up to 1æm (or more) into the nucleus. The filaments are curved and connect single or neighboring pores. The intranuclear filaments are associated with chromatin fibers and related to RNP particles of 20-25 nm and spheroidal structures of 0.5æm, with variations. The aggregates of several neighboring pores with the filaments are more commonly observed in 4n nuclei. We concluded that the intranuclear filaments may correspond to the fibrillar network described in Xenopus oocyte nucleus being probably related to RNA transport. The molecular basis of this RNA remains elusive. Nevertheless, the morphological aspects of the spheroidal structures indicate they could correspond to nucleolar chromatin or to nucleolus-derived structures. We also speculate whether the complex aggregates of neighboring pores with intranuclear filaments may correspond to pore clustering previously described in these tetraploid animals using freeze-etching experiments.

Filamentos ligados a poros foram visualizados em núcleos de espermatócitos de anuros através da técnica de espalhamento para microscopia eletrônica de transmissão. Os animais usados pertencem ao gênero Odontophrynus com espécies cripticas diplo e tetraplóides naturais, tendo os tetraplóides atividade metabólica reduzida. Os filamentos com 20-40 nm de largura são ligados a um anel componente do complexo poro nuclear de 90-120 nm e estendem-se até 1 æm (ou mais) para dentro do núcleo. Os filamentos são curvos e ligam poros simples ou poros vizinhos. Os filamentos intranucleares são associados a fibras de cromatina e relacionados a partículas de RNP de 20-25 nm e a estruturas esféricas de 0.5æm, com variações. Os agregados de poros vizinhos com os filamentos longos são mais freqüentemente observados em núcleos 4n. Concluímos que os filamentos intranucleares podem corresponder aos emaranhados fibrilares descritos em núcleos de oócitos de Xenopus e possivelmente relacion ados ao transporte de RNA. A base molecular desse RNA não é conhecida. Contudo, os aspectos morfológicos das estruturas esféricas parecem indicar que elas podem corresponder à cromatina nucleolar ou a estruturas derivadas do nucléolo. Também, especulamos se os agregados complexos de poros vizinhos com os filamentos intranucleares podem corresponder aos aglomerados de poros previamente descritos nesses animais tetraplóides através da técnica "freeze-etching".
Descritores: Anuros
Cromatina/ultraestrutura
Poro Nuclear/ultraestrutura
Espermatócitos/ultraestrutura
-Microscopia Eletrônica de Transmissão
Transporte de RNA
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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