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Pesquisa : A11.284.430.214.190.750.050 [Categoria DeCS]
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Yano, Tomomasa
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Id: biblio-1154774
Autor: Aragão, Annelize Zambon Barbosa; Quel, Natália Galdi; Joazeiro, Paulo Pinto; Yano, Tomomasa.
Título: Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts
Fonte: J. venom. anim. toxins incl. trop. dis;27:e20200106, 2021. tab, graf, ilus.
Idioma: en.
Projeto: State of São Paulo Research Foundation.
Resumo: Avian pathogenic Escherichia coli (APEC) isolated from avian cellulitis lesions produces a toxin, named Escherichia coli vacuolating factor (ECVF), that causes cell vacuolization and induces inflammatory response in broiler chicken. Methods We investigated the intracellular activities of ECVF in avian fibroblasts using fluorescence staining, electron microscopy, MTT and LDH measurements. As ECVF act specifically in avian cells, we performed blotting assay followed by mass spectrometry to better understand its initial intracellular protein recognition. Results ECVF induced actin contraction, mitochondrial damage and membrane permeability alterations. Ultrastructural analysis showed intracellular alterations, as nuclear lobulation and the presence of degraded structures inside the vacuoles. Moreover, ECVF induced cell death in fibroblasts. ECVF-biotin associates to at least two proteins only in avian cell lysates: alpha-actinin 4 and vinculin, both involved in cytoskeleton structure. Conclusion These findings demonstrated that ECVF plays an important role in avian cellulitis, markedly in initial steps of infection. Taken together, the results place this toxin as a target for drug and/or vaccine development, instead of the use of large amounts antibiotics.(AU)
Descritores: Vacúolos
Citoesqueleto de Actina
Galinhas
Actinas
Escherichia coli
Fibroblastos
-Celulite (Flegmão)
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1134469
Autor: Vásquez, Bélgica; del Sol, Mariano.
Título: Morfología neuronal en el trastorno del espectro autista / Neuronal morphology in autism spectrum disorder
Fonte: Int. j. morphol;38(5):1513-1518, oct. 2020.
Idioma: es.
Resumo: RESUMEN: El trastorno del espectro autista (TEA) abarca un grupo de trastornos multifactoriales del neurodesarrollo caracterizados por una comunicación e interacción social deteriorada y por comportamientos repetitivos y estereotipados. Múltiples estudios han revelado que en el TEA existen disfunciones sinápticas, en la cual la morfología y función neuronal son sustratos importantes en esta patogenia. En esta revisión comentamos los datos disponibles a nivel de anormalidades neuronales en el TEA, enfatizando la morfología de las dendritas, espinas dendríticas y citoesquelo de actina. Las dendritas y espinas dendríticas, ricas en actina, forman la parte postsináptica de la mayoría de las sinapsis excitadoras. En el TEA, los datos obtenidos apuntan a una desregulación en el crecimiento y desarrollo dendrítico, así como una alteración en la densidad de las espinas dendríticas. Lo anterior, se ve acompañado de alteraciones en la remodelación y composición del citoesqueleto neuronal. Para comprender mejor la fisiopatología del TEA, es necesario mayor información sobre cómo los cambios morfofuncionales de los actores que participan en la sinapsis impactan en los circuitos y el comportamiento.

SUMMARY: Autism Spectrum Disorder (ASD) is a group of multifactorial neurodevelopmental disorders, characterized by impaired communication and social interaction skills, and by repetitive and stereotyped behaviors. Multiple studies report that there are synaptic dysfunctions in ASD, in which important substrates such as morphology and neuronal function are involved in this pathogenesis. In this review we discuss the data available at the level of neuronal abnormalities in ASD, and emphasize the morphological aspects of dendrites, dendritic spines, and actin cytoskeleton. Actin-rich dendrites and dendritic spines shape the postsynaptic part of the most excitatory synapses. In ASD, the data points to a dysregulation in dendritic growth and development, as well as an alteration in the density of dendritic spines. This is accompanied by alterations in the remodeling and composition of the neuronal cytoskeleton. In order to better understand the pathophysiology of ASD, further information is needed on how the elements of synaptic morphofunctional changes impact circuits and behavior.
Descritores: Dendritos/patologia
Transtorno do Espectro Autista/patologia
-Citoesqueleto de Actina/patologia
Espinhas Dendríticas/patologia
Transtorno do Espectro Autista/fisiopatologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Timenetsky, Jorge
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Id: biblio-950734
Autor: Amorim, Aline Teixeira; Marques, Lucas Miranda; Santos, Angelita Maria Oliveira Gusmão; Martins, Hellen Braga; Barbosa, Maysa Santos; Rezende, Izadora Souza; Andrade, Ewerton Ferraz; Campos, Guilherme Barreto; Lobão, Tássia Neves; Cortez, Beatriz Araujo; Monezi, Telma Alvez; Machado-Santelli, Glaucia Maria; Timenetsky, Jorge.
Título: Apoptosis in HEp-2 cells infected with Ureaplasma diversum
Fonte: Biol. Res;47:1-9, 2014. ilus, graf.
Idioma: en.
Projeto: CNPq; . FAPESB; . FAPESP.
Resumo: BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.
Descritores: Ureaplasma/patogenicidade
Infecções por Ureaplasma/fisiopatologia
Apoptose/fisiologia
-Fatores de Tempo
Ureaplasma/efeitos dos fármacos
Aderência Bacteriana
Citoesqueleto de Actina/ultraestrutura
Gentamicinas/farmacologia
Células HeLa/microbiologia
Expressão Gênica
Sobrevivência Celular
Fator de Necrose Tumoral alfa/metabolismo
Estatísticas não Paramétricas
Microscopia Confocal
Caspase 3/metabolismo
Caspase 2/metabolismo
Caspase 9/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Padrões Moleculares Associados a Patógenos/metabolismo
Limites: Humanos
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1007407
Autor: Pereira, Túlio Felipe.
Título: Role of Ectodermal-neural cortex 1 protein in human glioma progression, identification of a peptide internalized by human glioblastoma cells and development of an alternative method to generate growth curves of adherent cultures / Papel da proteína Ectodermal-neural cortex 1 (ENC1) na progressão de glioma humano, identificação de um peptídeo internalizado por células de glioblastoma humano e desenvolvimento de um método alternativo para gerar curvas de crescimento celular.
Fonte: São Paulo; s.n; 2019. 85 p. tab, graf.
Idioma: en.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química para obtenção do grau de Doutor.
Resumo: Gliomas are the most common form of primary intracranial malignancy, among which astrocytomas are the most frequent. Ectodermal-cortex protein 1 (ENC 1), also known as Nuclear Restricted Protein/Brain (NRP/B), was first characterized as a protein which interacts with the cytoskeleton by binding to actin through Kelch-like domains, being related to neural fate specification during development of the nervous system. The first chapter of this thesis confirms ENC1 as a tumor suppression properties by a genomic edition approach, analyses ENC1 expression in a set of patient glioma samples and describes the correlation these data with patients survival and progression-free survival, concluding that ENC1 expression may constitute a biomarker for glioma aggressiveness. The second chapter refers to the identification and in vitro characterization of the LHTNELQ peptide, which was selected by the Phage Display method using human glioblastoma cells. This new peptide is able to be internalized by these cells and features as a new tool for the development of glioma therapeutics. The third chapter report an alternative method to generate growth curves of adherent cell cultures, which is based on the CFSE fluorescence decay over time. It is an alternative method to determine growth curves of cultured cells, with smaller variation among technical replicates than that of counting-based methods

Gliomas são a forma mais comum de malignidades primárias intracranianas, dentre os quais os astrocitomas são os mais frequentes. A proteína Ectodermal-neural cortex 1 (ENC1), também conhecida como Nuclear Restricted Protein/Brain (NRP/B), foi primeiramente caracterizada como uma proteína que interage com o citoesqueleto por meio de ligação à actina através de domínios Kelch-like, sendo relacionada com diferenciação neuronal durante o desenvolvimento do sistema nervoso. O primeiro capítulo desta tese descreve confirmação da capacidade supressora tumoral de ENC1 por abordagem de edição genômica, analisa a expressão de ENC1 em um conjunto de amostras de pacientes com gliomas e correlaciona esses dados com tempo de sobrevida geral e sobrevida livre de progressão tumoral nos pacientes, concluindo que a expressão de ENC1 pode ser utilizada como um biomarcador da agressividade do glioma. O segundo capítulo apresenta a identificação e caracterização in vitro do peptídeo LHTNELQ, que foi selecionado pela metodologia de Phage display utilizandose de células de glioblastoma humano. Este novo peptídeo é capaz de internalizar-se nestas células e figura como uma nova ferramenta para o desenvolvimento de estratégias terapêuticas para glioblastomas. No terceiro capítulo propõe-se um método alternativo para gerar curvas de crescimento celular de cultura aderente, o qual é baseado no decaimento da fluorescência do reagente CFSE ao longo do tempo. Tratase de um método alternativo para a determinação de curvas de crescimento de culturas aderentes, com menor variação entre as réplicas técnicas do que os métodos baseados em contagem das células
Descritores: Processos de Crescimento Celular
Fluorescência
Glioma/diagnóstico
-Citoesqueleto de Actina/classificação
Glioblastoma
Proteína 1 Associada a ECH Semelhante a Kelch/efeitos adversos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T574.88, P436pa. 30100026276-Q


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Id: biblio-870983
Autor: Dinardo, Carla Luana.
Título: Estudo das propriedades mecânicas das células de músculo liso vascular em situações fisiológicas e patológicas / Study of the mechanical properties of vascular smooth muscle cells under physiological and pathological situations.
Fonte: São Paulo; s.n; 2015. [203] p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: Introdução: As células do músculo liso vascular (CMLV) são quiescentes nos vasos adultos, com baixa capacidade de migração e de secreção de matriz extracelular, caracterizando fenótipo contrátil. Evidências apontam para a heterogeneidade fenotípica das CMLV ao longo da árvore arterial: há distribuição heterogênea de doenças e de resposta a determinadas drogas nos diferentes vasos, além de variabilidade de expressão dos genes de proteínas contráteis de músculo liso entre eles. O papel das CMLV, em fase adulta, é classicamente descrito como restrito à regulação do tônus de pequenos vasos, sendo insignificante a contribuição da mecânica das CMLV para a complacência das artérias elásticas. Existe a hipótese de que a viscoelasticidade das CMLV contribua para a mecânica final das artérias, sendo o enrijecimento dessas células associado à rigidez arterial. Objetivo: Estudar a variabilidade das propriedades mecânicas e de expressão proteica das CMLV, ao longo da árvore arterial, buscando identificar moduladores regionais para esse fenótipo. Avaliar se situações clínicas sabidamente associadas à rigidez arterial (envelhecimento, sexo feminino pós-menopausa, ancestralidade genética africana, diabetes mellitus e tabagismo) cursam com enrijecimento de CMLV. Métodos: 1) Estudou-se a composição e a organização da camada média de diferentes artérias. As CMLV desses vasos foram avaliadas quanto à viscoelasticidade de citoplasma (G), por meio do ensaio de Citometria Magnético Ótica de Oscilação e, quanto à expressão proteica global, usando cromatografia multidimensional e espectrometria de massas em tandem de alta resolução (Proteômica Shotgun). Os dados mecânicos obtidos foram correlacionados com as características da matriz extracelular (MEC) dos vasos de origem (porcentagem de elastina e quantidade de MEC). Em paralelo, foi realizado experimento de estiramento cíclico (10%/1Hz) das CMLV das diferentes artérias por 24 e 48h, seguido pela mensuração de rigidez de...

Rational: Vascular smooth muscle cells (VSMC) lose their ability to migrate and secrete extracellular matrix (ECM) with the end of vascular development, condition known as contractile phenotype and reversible in the presence of vascular injury. There is evidence of heterogeneity of VSMC phenotype along arterial tree, as the distribution of diseases (atherosclerosis) and the response to drugs vary between different vessels, as well as the expression of smooth muscle-contractile protein genes. The role played by VSMC mechanics on determining large arteries' compliance was always considered irrelevant. It has been hypothesized that the VSMC mechanical properties are important for vascular mechanics, especially in the pathological scenario, where VSMC stiffening may be associated with arterial rigidity. Goals: Study the variation of VSMC mechanics and protein expression along arterial tree, identifying regional modulators of this phenotype. Evaluate if clinical situations associated with arterial rigidity (ageing, post-menopausal women, African ancestry, diabetes mellitus and smoking) concur with VSMC stiffening. Methods: 1) Different arteries were studied in terms of composition and organization of their media layer. VSMC isolated from these arteries were evaluated regarding cytoplasm viscoelasticity, measured using Optical Magnetic Twisting Cytometry Assay (OMTC), and protein expression, using two-dimensional liquid chromatography and tandem mass spectrometry (Shotgun Proteomics). Mechanical data were correlated with ECM characteristics (percentage of elastin and ECM amount) of the vessels of origin. In parallel, VSMC of different arteries were subjected to cyclic stretching (10%/1Hz) during 24 and 48h, followed by the measurement of their cytoplasm rigidity. 2) VSMC were isolated from fragments of mammary artery of 80 patients subjected to coronary bypass surgery and evaluated regarding their viscoelasticity (G, G' e G''). A statistic model was elaborated to...
Descritores: Citoesqueleto de Actina
Artérias
Citoesqueleto
Músculo Liso
Proteômica
Fumar
Rigidez Vascular
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


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Id: lil-676820
Autor: Sun, Xuerong; Liu, Xinguang; Zhang, Yuehong; Kuang, Xielan; Lv, Bo; Ge, Jian.
Título: A simple and effective pressure culture system modified from a transwell cell culture system
Fonte: Biol. Res;46(1):47-52, 2013. ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . State Key Laboratory of Ophthalmology. Fundamental Research Funds; . Medical Scientific Research Foundation of Guangdong Province; . Guangdong Medical College. Science & Technology Innovation Fund.
Resumo: Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
Descritores: /genética
ANTIGENS, CDABORTION, INCOMPLETE/genética
Proliferação de Células
Técnicas de Cultura de Células/métodos
-Análise de Variância
Citoesqueleto de Actina/fisiologia
Linhagem Celular/fisiologia
Fibroblastos/fisiologia
Imunofluorescência/métodos
Pressão Hidrostática
Metilaminas
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Neoplasias Nasofaríngeas/patologia
Cultura Primária de Células
Reação em Cadeia da Polimerase em Tempo Real
Células Ganglionares da Retina/citologia
Células Ganglionares da Retina/fisiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Estresse Mecânico
Limites: Animais
Humanos
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-644246
Autor: Silveira, Marina; Aragão, Pedro Henrique Arruda.
Título: Organized Filaments in the adhesive system of Macrostomum tuba Graff, 1882 ( Platyhelminthes, Macrostomida)
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;23(3/4):471-477, July-Dec. 2006. ilus.
Idioma: en.
Resumo: The adhesive organs or “duo-gland adhesive organs” of platyhelminths are formed by a specialized epithelialcell and extensions of two gland cells. These organs are used for temporary fixation of the organism tosurfaces in aquatic habitats. The mechanisms involved in adhesion to and release from a given surfacedepend on secretions produced by the glands; less is known about the involvement of cytoplasmic filamentsin the anchoring cell itself. In this study, we examined the structure of the adhesive organs present in thetail plate of Macrostomum tuba Graff, 1882 (Platyhelminthes, Macrostomida), a freshwater, free-livingflatworm. Scanning and transmission electron microscopy allowed elucidation of the three-dimensionalorganization of the adhesive system, especially of the microvilli that formed the outer collar (or papilla),which was endowed with a fibrous core. Electrical stimulation caused the flatworms to extend their papillaeabove the ciliated surface. The use of tannin- and diamine-containing fixatives showed that the filamentousarray contained tonofilaments and actin filaments. Tonofilaments concentrate in the axis of each microvillus;actin filaments, about 7-8 nm thick, spread out towards the periphery. Scanning images demonstrated thefinger-like shape of the papillae, about 7-8 ìm high, with a terminal opening. Microvilli followed a straightcourse along the surface.
Descritores: Citoesqueleto de Actina
Actinas
Citoesqueleto de Actina/ultraestrutura
Platelmintos/anatomia & histologia
-Diaminas
Platelmintos
Platelmintos/fisiologia
Limites: Animais
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL


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Id: lil-600693
Autor: Goulart-Silva, F; Serrano-Nascimento, C; Nunes, M. T.
Título: Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(10):1060-1067, Oct. 2011. ilus, tab.
Idioma: en.
Resumo: The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led us to measure: a) proinsulin mRNA expression, b) proinsulin transcripts and eEF1A protein binding to the actin cytoskeleton, c) actin cytoskeleton arrangement, and d) proinsulin mRNA poly(A) tail length modulation in INS-1E cells cultured in different media containing: i) normal fetal bovine serum - FBS (control); ii) normal FBS plus 1 µM or 10 nM T3, for 12 h, and iii) FBS depleted of TH for 24 h (Tx). A decrease in proinsulin mRNA content and attachment to the cytoskeleton were observed in hypothyroid (Tx) beta cells. The amount of eEF1A protein anchored to the cytoskeleton was also reduced in hypothyroidism, and it is worth mentioning that eEF1A is essential to attach transcripts to the cytoskeleton, which might modulate their stability and rate of translation. Proinsulin poly(A) tail length and cytoskeleton arrangement remained unchanged in hypothyroidism. T3 treatment of control cells for 12 h did not induce any changes in the parameters studied. The data indicate that TH is important for proinsulin mRNA expression and translation, since its total amount and attachment to the cytoskeleton are decreased in hypothyroid beta cells, providing evidence that effects of TH on carbohydrate metabolism also include the control of proinsulin gene expression.
Descritores: Citoesqueleto de Actina/metabolismo
Fator de Iniciação 1 em Eucariotos/metabolismo
Hipotireoidismo/metabolismo
Células Secretoras de Insulina/metabolismo
Proinsulina/genética
RNA Mensageiro/metabolismo
-Expressão Gênica
Hipotireoidismo/genética
Proinsulina/biossíntese
RNA Mensageiro/genética
Limites: Animais
Bovinos
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-541117
Autor: Capani, F; Saraceno, E; Boti, V. R; Aon-Bertolino, L; Fernández, J. C; Gato, F; Krause, M. S; Giraldez, L; Ellisman, M. H; Coirini, H.
Título: A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
Fonte: Biocell;32(1):1-8, Apr. 2008. ilus.
Idioma: en.
Resumo: Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.
Descritores: Actinas/metabolismo
Amarelo de Eosina-(YS)/farmacologia
Amarelo de Eosina-(YS)/metabolismo
Foto-Oxidação
Sistema Nervoso Central/metabolismo
Sistema Nervoso Central/ultraestrutura
-Coloração e Rotulagem/métodos
Corantes Fluorescentes/farmacologia
Faloidina/farmacologia
Imageamento Tridimensional/métodos
Modelos Moleculares
Citoesqueleto de Actina/metabolismo
Citoesqueleto de Actina/ultraestrutura
Microscopia de Fluorescência/métodos
Oxirredução
Fótons
Limites: Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


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Mermelstein, Claudia dos Santos
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Id: lil-269386
Autor: Mermelstein, Claudia dos Santos; Costa, Manoel Luis; Moura Neto, Vivaldo.
Título: The cytoskeleton of the electric tissue of Electrophorus electricus, L
Fonte: An. acad. bras. ciênc;72(3):341-51, Sept. 2000. ilus, tab.
Idioma: en.
Resumo: The electric eel Electrophorus electricus is a fresh water teleost showing an electrogenic tissue that produces electric discharges. This electrogenic tissue is distributed in three well-defined electric organs which may be found symmetrically along both sides of the eel. These electric organs develop from muscle and exhibit several biochemical properties and morphological features of the muscle sarcolema. This review examines the contribution of the cytoskeletal meshwork to the maintenance of the polarized organization of the electrocyte, the cell that contains all electric properties of each electric organ. The cytoskeletal filaments display an important role in the establishment and maintenance of the highly specialized membrane model system of the electrocyte. As a muscular tissue, these electric organs expresses actin and desmin. The studies that characterized these cytoskeletal proteins and their implications on the electrophysiology of the electric tissues are revisited.
Descritores: Citoesqueleto/química
Órgão Elétrico/química
Electrophorus/fisiologia
-Citoesqueleto de Actina/química
Citoesqueleto de Actina/fisiologia
Citoesqueleto de Actina/ultraestrutura
Citoesqueleto/metabolismo
Citoesqueleto/ultraestrutura
Densitometria
Órgão Elétrico/fisiologia
Órgão Elétrico/ultraestrutura
Eletroforese em Gel Bidimensional
Microscopia Eletrônica
Limites: Animais
Tipo de Publ: Revisão
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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