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Pesquisa : A11.284.430.214.190.875.336 [Categoria DeCS]
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Id: biblio-1025571
Autor: Ortiz, Kevin; Álvarez, Egly; Herrera, Maynor.
Título: Patrón sugestivo de anticuerpos contra el aparato de Golgi en un paciente con uveítis / Sugestive pattern of anti-Golgi complex antibodies in a patient with uveitis
Fonte: Cienc. tecnol. salud;6(1):77-81, 2019. ilus.
Idioma: es.
Resumo: Los anticuerpos dirigidos contra el aparato de Golgi fueron inicialmente descritos en un paciente con Síndrome de Sjögren en 1982. Estos anticuerpos forman parte de los anticuerpos antinucleares y producen un patrón característico en las células Hep-2. Desde su descubrimiento no se ha logrado establecer ninguna asociación clara con alguna enfermedad autoinmune y/o manifestación clínica. En el presente artículo se reporta el primer caso de anticuerpos antinucleares por fluorescencia (Fana) positivo con un patrón polar/sugestivo de anti-Golgi en Guatemala encontrado en el Laboratorio de Inmunología-Autoinmunidad del Hospital Roosevelt en un paciente masculino con una uveítis en el ojo derecho y que presentó pruebas de laboratorio positivas para toxoplasma, rubeola IgG, citomegalovirus, y herpes 1 y 2. Este patrón ha sido encontrado en personas con diferentes enfermedades autoinmunes pero no se ha logrado establecer asociación con alguna enfermedad en particular.

The anti-Golgi complex antibodies were first described in a patient with Sjögren Syndrome in 1982. These antibodies are part of the antinuclear antibodies and they have a characteristic staining pattern in Hep-2 cells. They have not been associated with any autoimmune disease and/or clinical manifestation. In the present case we report the first nuclear antibodies (ANA) with a staining pattern polar/anti-Golgi-like founded in the Immunology-Autoimmunity Laboratory at Roosevelt Hospital in a male patient with an uveitis on the right eye and positive IgG serology for toxoplasma, rubella, cytomegalovirus and herpes 1 and 2. This pattern has been founded in patients with different autoimmune diseases, but they haven´t been associated with a disease.
Descritores: Uveíte/complicações
Autoimunidade
Complexo de Golgi/microbiologia
-Vírus da Rubéola
Toxoplasma
Uveíte/microbiologia
Acuidade Visual
Citomegalovirus
Limites: Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: GT49.1


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Taniwaki, Noemi Nosomi
Carneiro, Sylvia Mendes
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Id: lil-736357
Autor: Sesso, Antonio; Yamashiro-Kanashiro, Edite Hatsumi; Orii, Noemia Mie; Taniwaki, Noemi Nosomi; Kawakami, Joyce; Carneiro, Sylvia Mendes.
Título: Loose and compatt agglomerates of 50 nm microveicles derived from golgi and enndoplasmic reticulum membranes in pre- and in -apoptotic mycoplasma infected hela cells: host-parasite interactions under the transmission electron microscope
Fonte: Rev. Inst. Med. Trop. Säo Paulo;57(1):89-91, Jan-Feb/2015. graf.
Idioma: en.
Descritores: Retículo Endoplasmático/ultraestrutura
Complexo de Golgi/microbiologia
Mycoplasma/fisiologia
-Membrana Celular/microbiologia
Membrana Celular/ultraestrutura
Retículo Endoplasmático/microbiologia
Complexo de Golgi/ultraestrutura
Células HeLa/microbiologia
Células HeLa/ultraestrutura
Interações Hospedeiro-Parasita/fisiologia
Microscopia Eletrônica de Transmissão
Mycoplasma/ultraestrutura
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: lil-233895
Autor: Vieira, Marcus Fraga; Kohn, André Fábio.
Título: Reconhecimento de padröes no cerebelo: dinâmica do sistema Golgi-célula nuclear / Recognition device in cerebellum: dynamics of the Golgi-granule cell system
Fonte: In: Schiabel, Homero; Slaets, Annie France Frère; Costa, Luciano da Fontoura; Baffa Filho, Oswaldo; Marques, Paulo Mazzoncini de Azevedo. Anais do III Fórum Nacional de Ciência e Tecnologia em Saúde. Säo Carlos, s.n, 1996. p.614-614, ilus.
Idioma: pt.
Conferência: Apresentado em: Fórum Nacional de Ciência e Tecnologia em Saúde, 3 e Congresso Brasileiro de Engenharia Biomédica, 15 e Congresso Brasileiro de Físicos em Medicina , 6 e Congresso Brasileiro de Informática em Saúde, 5 e Encontro Brasileiro de Proteçäo Radiológica, Campos do Jordäo, 13-17 out. 1996.
Resumo: O grande esforço no sentido de organizar em uma base teórica sólida o enorme volume de dados a respeito do funcionamento cerebelar, tem levado à elaboração de inúmeros modelos teóricos. Neste aspecto, a abordagem mais utilizada é provavelmente a decrição do cerebelo como um dispositivo de reconhecimento de padrões. Para isto enfatiza-se que é fundamentalmente o conhecimento da dinâmica do sistema Golgi-célula granular, que possivelmente também pode exibir propriedades de plasticidade sináptica.
Descritores: Cerebelo/fisiologia
Complexo de Golgi
-Sinapses
Fibras Nervosas
Células de Purkinje
Córtex Cerebelar
Responsável: BR1.1 - BIREME
BR1.1/3012.87


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Texto completo SciELO Chile
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Id: biblio-893000
Autor: Gutiérrez-Cantú, Francisco Javier; Guerrero-Barrera, Alma Lilián; Sánchez Meraz, Wulfrano; Pozos-Guillen, Amaury de Jesús; Flores-Reyes, Héctor; Gutiérrez Robles, Eduardo Alejandro; Mariel Murga, Humberto; Romo Ramírez, Gabriel Fernando; Mariel Cárdenas, Jairo.
Título: Expression of enamel proteins in rough endoplasmic reticulum and golgi complex in human dental germs / Expresión de proteínas del esmalte en retículo endoplásmico rugoso y complexo golgiensis en gérmenes dentales humanos
Fonte: Int. j. morphol;35(2):435-441, June 2017. ilus.
Idioma: en.
Resumo: Tooth enamel is the hardest tissue in the body. The organic matrix configuration is provided by the main proteins amelogenin, ameloblastin and enamelysin (MMP20), an enzyme that helps to shape the matrix. The aim of this study was to determine by histochemistry the expression of amelogenin and enamelysin through the rough endoplasmic reticulum in the late stages of amelogenesis, and its expression in the Complexus golgiensis (Golgi complex / Golgi apparatus) in the early stages in human fetuses. In early stages a colocalization of both proteins inside the Golgi apparatus was found, being more evident the relationship between Golgi and amelogenin (99.92 %). In the late stage, a colocalization of both proteins and rugged endoplasmic reticulum was found. With enamelysin being more evident in relation with rough endoplasmic reticulum (99.95 %). Our findings demonstrated the presence of amelogenin and enamelysin in odontoblast and ameloblast. However, the presence of these two proteins in odontoblast remains unknown.

El esmalte dental es el tejido más duro del cuerpo. La configuración de la matriz orgánica es proporcionada por las proteínas principales amelogenina, ameloblastina y enamelisina (MMP20), una enzima que ayuda a dar forma a la matriz. El objetivo de este estudio fue determinar mediante histoquímica la expresión de amelogenina y enamelisina a través del retículo endoplasmático rugoso en las últimas etapas de la amelogénesis , y su expresión en el Complexo golgiensis en las primeras etapas de formación en fetos humanos. En las primeras etapas se observó colocalización de ambas proteínas en el interior del Complexo golgiensis, siendo más evidente la relación entre Golgi y amelogenina (99,92 %). En la última etapa, se identificó una colocalización de ambas proteínas y retículo endoplásmico rugoso. Resulto más evidente la enamelisina en relación con el retículo endoplasmático rugoso (99,95 %). Nuestros resultados demostraron la presencia de amelogenina y enamelisina en odontoblastos y ameloblastos, sin embargo se desconoce la presencia de estas dos proteínas en odontoblastos.
Descritores: Amelogenina/metabolismo
Proteínas do Esmalte Dentário
Retículo Endoplasmático Rugoso
Complexo de Golgi
Metaloproteinase 20 da Matriz/metabolismo
-Amelogênese
Imunofluorescência
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Texto completo
Id: lil-718194
Autor: Souza, Romeu R. de; Oliveira, Vanessa C. de; Curi, Tania Cristina P.; Maldonado, Diogo C..
Título: Effects of ovariectomy on the secretory apparatus in the right atrial cardiomyocytes of middle-aged mice
Fonte: Clinics;69(8):554-558, 8/2014. tab, graf.
Idioma: en.
Resumo: OBJECTIVE : The aim of the present study was to evaluate the effects of ovariectomy on the secretory apparatus of natriuretic peptides in right atrial cardiomyocytes. METHODS: Nine-month-old mice underwent bilateral ovariectomy or sham surgery. The blood exam of the ovariectomized mice showed results consistent with castrated females. Systolic blood pressure was measured after ovariectomy (9 mo of age) and at the moment of sacrifice (12 mo of age). Fragments of the right atrium were collected and prepared for electron microscopy examination. The following variables were quantified: the quantitative density and area of the natriuretic peptide granules, the relative volume of euchromatin in the nucleus, the number of pores per 10 μm of the nuclear membrane and the relative volumes of the mitochondria and Golgi complex. RESULTS: The cardiomyocytes obtained from ovariectomized mice indicated that the quantitative density and the area of secretory granules of natriuretic peptides were significantly lower compared with the sham-operated mice. Furthermore, there was a decrease in the relative volume of euchromatin, a lower density of nuclear pores, and lower relative volumes of the mitochondria and Golgi complex in the ovariectomized mice compared with the sham-operated mice. These findings suggest a pool with a low turnover rate, i.e., low synthesis and elimination of natriuretic peptides. CONCLUSION: A lack of estrogen caused hypotrophy of the secretory apparatus in right atrial cardiomyocytes that could explain the weak synthesis of natriuretic peptides in mice. Furthermore, one of the mechanisms of blood pressure control was lost, which may explain, in part, the elevated blood pressure in ovariectomized mice. .
Descritores: Fator Natriurético Atrial/efeitos dos fármacos
Miócitos Cardíacos/ultraestrutura
Ovariectomia/efeitos adversos
-Fator Natriurético Atrial/análise
Pressão Sanguínea
Estradiol/sangue
Estrogênios/fisiologia
Eucromatina/ultraestrutura
Complexo de Golgi/ultraestrutura
Átrios do Coração/citologia
Tamanho Mitocondrial
Modelos Animais
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Poro Nuclear/ultraestrutura
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-595016
Autor: Villalobos, Virginia; Bonilla, Ernesto; Castellano, Alan; Novo, Ernesto; Caspersen, Ralph; Giraldoth, Debora; Medina-Leendertz, Shirley.
Título: Ultrastructural changes of the olfactory bulb in manganese-treated mice
Fonte: Biocell;33(3):187-197, Dec. 2009. ilus.
Idioma: en.
Resumo: The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl2 (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and irregularly shaped nuclei with abnormal chromatin distribution were observed after 6 weeks. Some glial cells showed disorganization of the Golgi apparatus. On week 9, an increase in the number of astrocytes, whose mitochondrial cristae were partially or totally erased, and a dilation of the rough endoplasmic reticulum were found. Neurons appear degenerated, with swollen mitochondria and a vacuolated, electron dense cytoplasm. These changes seem to indicate that the olfactory bulb is sensitive to the toxic effects of manganese.
Descritores: Complexo de Golgi
Complexo de Golgi/ultraestrutura
Astrócitos
Astrócitos/ultraestrutura
Cloretos/toxicidade
Retículo Endoplasmático Rugoso
Retículo Endoplasmático Rugoso/ultraestrutura
-Bulbo Olfatório
Bulbo Olfatório/ultraestrutura
Compostos de Manganês
Microscopia Eletrônica de Transmissão
Mitocôndrias
Mitocôndrias/ultraestrutura
Neuroglia
Neuroglia/ultraestrutura
Neurônios
Neurônios/ultraestrutura
Limites: Masculino
Animais
Camundongos
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


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Id: lil-478223
Autor: Torres-Fernández, Orlando; Yepes, Gloria E; Gómez, Javier E.
Título: Alteraciones de la morfología dendrítica neuronal en la corteza cerebral de ratones infectados con rabia: un estudio con la técnica de Golgi / Neuronal dentritic morphology alterations in the cerebral cortex of rabies-infected mice: a Golgi study
Fonte: Biomédica (Bogotá);27(4):605-613, dic. 2007. ilus, tab.
Idioma: es.
Resumo: Introducción. Los signos neurológicos de la rabia son impresionantes; no obstante, el cerebro infectado sufre apenas cambios histológicos muy sutiles. Objetivo. Estudiar la morfología neuronal mediante la técnica de Golgi, en la corteza cerebral de ratones infectados con el virus de la rabia. Materiales y métodos. Se inocularon ratones con virus silvestre de la rabia (virus 'calle') de origen canino o con virus adaptado (virus 'fijo') de la cepa CVS (challenge virus standard). Los animales se sacrificaron en la fase terminal de la enfermedad y se fijaron por perfusión con paraformaldehído. Los cerebros se procesaron con la técnica de Golgi, se obtuvieron cortes coronales de la corteza, se contaron las neuronas impregnadas en un área de 1 mm2, se midió el tamaño de sus cuerpos celulares y se tomaron fotografías en diferentes planos de profundidad. Resultados. Se observaron alteraciones morfológicas notables en el soma y las dendritas de neuronas piramidales, con pérdida acentuada de espinas, en 12,9 por ciento de neuronas corticales de animales infectados con virus 'calle' por vía intracerebral; en 8,2 por ciento de neuronas de ratones inoculados con este mismo virus por la ruta intramuscular y en 31,8 por ciento de neuronas en los animales inoculados con virus 'fijo' por vía intramuscular. Además, en las muestras de material infectado el número de neuronas impregnadas por la técnica de Golgi fue considerablemente menor al observado en las muestras no infectadas. Conclusiones. Estos resultados son evidencia de que el virus de la rabia sí puede inducir daño neuronal estructural. Además, esta infección aparentemente interfiere con los mecanismos de impregnación argéntica del método de Golgi.

Introduction. The neurological signs of rabies are very dramatic. Nevertheless, the infected brain manifests only very subtle histological changes. Objective. The neuronal morphology in the cerebral cortex of rabies-infected mice was examined by means the Golgi technique for detection of neuropathy. Materials and methods. Two groups of mice were inoculated with rabies—one with street virus isolated from an infected dog and the second with fixed CVS (challenge virus standard) virus. At the terminal phase of illness, the animals were sacrificed and fixed for histological staining by perfusion with paraformaldehyde. Next, the brains were treated by the Golgi technique and coronal sections were obtained. Neurons enclosed within 1 mm2 frames of the frontal cortex sections were counted and the sizes of the cellular bodies were measured. Photographs of several depth levels from the sections were obtained. Results. Cortical pyramidal neurons showed distinctive morphological alterations in the soma and dendrites (including loss of dendritic spines) in 12.9% of cells from intracerebral infectedmice with street virus; in 8.2% of neurons from intramuscular infected-mice with street virus, and in 31.8% of neurons from mice injected intramusculary with fixed virus. In addition, the number of neurons impregnated by the Golgi technique in infected brains was considerably lower than in the non-infected samples. Conclusions. Rabies virus can induce structural neuron damage. The infection also appears to induce tissue changes that interfere with the chemical mechanisms of the Golgi silver impregnation method.
Descritores: Córtex Cerebral
Complexo de Golgi
Neurônios
Vírus da Raiva
-Doenças do Sistema Nervoso
Limites: Camundongos
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


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Texto completo SciELO Brasil
Texto completo
Id: lil-477753
Autor: Sá, Maria José; Ruela, Carlos; Madeira, Maria Dulce.
Título: Dendritic right/left asymmetries in the neurons of the human hippocampal formation: a quantitative Golgi study
Fonte: Arq. neuropsiquiatr;65(4b):1105-1113, dez. 2007. ilus, graf.
Idioma: en.
Resumo: OBJECTIVE: To search for right/left asymmetries in the dendritic trees of the neuronal populations and in the cell-free layer volumes of the human hipoccampal formation. METHOD: In necropsic material obtained from six male individuals we performed a quantitative Golgi study of the dendritic trees of dentate granules, CA3 and CA1 pyramidal neurons and a volumetric analysis of dentate gyrus molecular layer, strata oriens plus alveus and strata lacunosum-moleculare plus radiatum of CA3 and CA1 fields. RESULTS: We found inter-hemispheric asymmetries in the dendrites trees of all neurons, reaching the significant level in the number of granule cells dendritic segments (higher in the left than in the right hemisphere), dendritic branching density of CA3 pyramidal cells and mean dendritic length of CA1 apical terminal segments (higher in the right than in the opposite side). No volumetric differences were observed. CONCLUSION: This study points to different anatomical patterns of connectivity in the hippocampal formations of both hemispheres which may underlie functional asymmetries.

OBJETIVO: Pesquisar a existência de assimetrias direita/esquerda nas arborizações dendríticas neuronais e nos volumes das camadas não celulares da formação do hipocampo humano. MÉTODO: Efectuamos estudo quantitativo Golgi das arborizações dendríticas dos grânulos da fascia denteada e das células piramidais de CA3 e CA1, e uma análise estereológica dos volumes da camada molecular da fascia denteada, do strata oriens + alveus e do strata lacunosum-moleculare + radiatum de CA3 e de CA1 em material necrópsico colhido em 6 indivíduos do sexo masculino. RESULTADOS: Encontrámos assimetrias inter-hemisféricas nas arborizações dendríticas de todos os neurónios, significativas no número de segmentos dendríticos das células granulares (maior à esquerda do que à direita) na densidade de ramificação dendrítica das pirâmides de CA3 e no comprimento dendrítico médio dos segmentos apicais terminais das pirâmides de CA1 (maiores à direita do que à esquerda). Não encontramos diferenças volumétricas. CONCLUSÃO: Estes resultados alertam para diferentes padrões anatómicos de conectividade nas formações do hipocampo de ambos os hemisférios que podem fundamentar assimetrias funcionais.
Descritores: Dendritos
Complexo de Golgi
Hipocampo/citologia
Neurônios/citologia
Células Piramidais/citologia
-Contagem de Células
Tamanho Celular
Coloração e Rotulagem/métodos
Limites: Adulto
Humanos
Masculino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-475405
Autor: Torres-Fernández, Orlando.
Título: La técnica de impregnación argéntica de Golgi. Conmemoración del centenario del premio nobel de Medicina (1906) compartido por Camillo Golgi y Santiago Ramón y Cajal / The Golgi silver impregnation method: commemorating the centennial of the Nobel Prize in Medicine (1906) shared by Camillo Golgi and Santiago Ramón y Cajal
Fonte: Biomédica (Bogotá);26(4):498-508, dic. 2006. ilus.
Idioma: es.
Resumo: La técnica de Golgi es un sencillo procedimiento histológico que revela la morfología neuronal completa en tres dimensiones. Este método se fundamenta en la formación de depósitos opacos intracelulares de cromato argéntico, producto de la reacción entre el bicromato de potasio y el nitrato de plata (reacción negra). Camillo Golgi, su descubridor, y Santiago Ramón y Cajal, su principal exponente, recibieron el premio nobel de Medicina y Fisiología en 1906 por su contribución al conocimiento de la estructura del sistema nervioso. Gran parte de sus logros se obtuvieron a través de la aplicación del método de impregnación argéntica. Sin embargo, Golgi y Cajal tenían interpretaciones diferentes sobre la estructura del tejido nervioso. Golgi era defensor de la teoría reticular, la cual proponía que el sistema nervioso estaba conformado por una red de células fusionadas a través de los axones a manera de un sincitio. Por el contrario, la doctrina neuronal, defendida por Cajal, sostenía que las neuronas eran células independientes. También se debe a Golgi y su reazione nera el descubrimiento del organelo celular conocido como 'aparato de Golgi'. La microscopía electrónica confirmó los postulados de la doctrina neuronal, así como la existencia del complejo de Golgi, y contribuyó al resurgimiento de la técnica de impregnación argéntica. Aunque existen métodos modernos de tinción intracelular que revelan imágenes excelentes de la morfología neuronal, la técnica de Golgi se mantiene vigente por ser un método más práctico y menos costoso para el estudio de la morfología normal y patológica de las neuronas.

Introduction. Gestational trophoblastic disease includes a group of pathologies characterized by abnormal trophoblast growth and invasion. The molecular bases of the disease are largely unknown, due in part to the lack of appropriate biological models. The insulin-like growth factor (IGF) system plays a fundamental role in the growth and development of many tissues and is involved in the progression of several diseases. Objectives. Primary cell cultures derived from first trimester placenta were characterized from patients with complete hydatidiform mole and spontaneous non molar abortion by immunocytochemical and molecular methods.Materials and Methods. The immunocytochemical method used specific markers for trophoblastic cells, whereas RT-PCR was used to identify insulin-like growth factor gene expression. Results. Histochemical staining with hematoxilin-eosin revealed that the cultures contained heterogeneous cell types, including trophoblast and endometrial decidual cells. The ratio of trophoblast cells in the cultures varied between 16% and 37%, as detected by cytokeratine-7 as the specific trophoblast marker. Gene expression analysis corroborated the presence of trophoblasts by detecting insulin-like growth factor II mRNA, whereas GH-V transcripts were correlated with the presence of syncitiotrophoblasts. Insulin-like growth factor I and insulin-like growth factor binding protein 1 mRNAs were related to mesenchyimal and decidual cells, respectively. Higher insulin-like growth factor II expression levels were found in molar tissues in comparison with non-molar abortions. Conclusion. By combining three methodologies—morphology, immunocytochemistry and gene expression, characterization and follow-up of placenta cultures from abnormal tissues is found to facilitate diagnosis.
Descritores: Técnicas Histológicas
Neuroanatomia
Prêmio Nobel
-Complexo de Golgi
História da Medicina
Microscopia Eletrônica
Tipo de Publ: Artigo Histórico
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


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Texto completo SciELO Chile
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Id: lil-468194
Autor: Toro, Andrés; Arredondo, Cristian; Cordova, Gonzalo; Araya, Claudia; Palacios, José L; Venegas, Alejandro; Morita, Masashi; Imanara, Tsuneo; Santos, Manuel J.
Título: Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells
Fonte: Biol. Res;40(2):231-249, 2007. ilus.
Idioma: en.
Projeto: FONDECYT; . Japan. Welfare and Labours. Ministry of Health. Research on Measures for Intractable Diseases.
Resumo: Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The expressed proteins were targeted to peroxisomes and peroxisomal ghosts correctly in the presence or absence of Brefeldin A (BFA), a drug known to block the ER-Golgi transit. Furthermore, low temperature did not disturb the targeting of Pex3p-GFP to peroxisomes. We also constructed two chimeric proteins of PMPs containing an ER retention signal "DEKKMP": GFP-ALDRP-DEKKMP and myc- Pex3p-DEKKMP. These proteins were mostly targeted to peroxisomes. No colocalization with an ER maker was found. These results suggest that the classical ER-Golgi pathway does not play a major role in the biogenesis of mammalian PMPs.
Descritores: Retículo Endoplasmático/fisiologia
Complexo de Golgi/fisiologia
Mutação
Proteínas de Membrana/metabolismo
Peroxissomos/metabolismo
-Células CHO
Cricetulus
Retículo Endoplasmático/metabolismo
Proteínas de Membrana/genética
Limites: Animais
Cricetinae
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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