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Pesquisa : A11.284.430.214.190.875.564.461 [Categoria DeCS]
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Id: biblio-950770
Autor: Soto-Urquieta, María G; López-Briones, Sergio; Pérez-Vázquez, Victoriano; Saavedra-Molina, Alfredo; González-Hernández, Gloria A; Ramírez-Emiliano, Joel.
Título: Curcumin restores mitochondrial functions and decreases lipid peroxidation in liver and kidneys of diabetic db/db mice
Fonte: Biol. Res;47:1-8, 2014. graf.
Idioma: en.
Resumo: BACKGROUND: Nitrosative and oxidative stress play a key role in obesity and diabetes-related mitochondrial dysfunction. The objective was to investigate the effect of curcumin treatment on state 3 and 4 oxygen consumption, nitric oxide (NO) synthesis, ATPase activity and lipid oxidation in mitochondria isolated from liver and kidneys of diabetic db/db mice. RESULTS: Hyperglycaemia increased oxygen consumption and decreased NO synthesis in liver mitochondria isolated from diabetic mice relative to the control mice. In kidney mitochondria, hyperglycaemia increased state 3 oxygen consumption and thiobarbituric acid-reactive substances (TBARS) levels in diabetic mice relative to control mice. Interestingly, treating db/db mice with curcumin improved or restored these parameters to normal levels; also curcumin increased liver mitochondrial ATPase activity in db/db mice relative to untreated db/db mice. CONCLUSIONS: These findings suggest that hyperglycaemia modifies oxygen consumption rate, NO synthesis and increases TBARS levels in mitochondria from the liver and kidneys of diabetic mice, whereas curcumin may have a protective role against these alterations.
Descritores: Peroxidação de Lipídeos/efeitos dos fármacos
Curcumina/farmacologia
Diabetes Mellitus Tipo 2/dietoterapia
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
-Consumo de Oxigênio/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/enzimologia
Adenosina Trifosfatases/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Respiração Celular/efeitos dos fármacos
Suplementos Nutricionais
Diabetes Mellitus Tipo 2/complicações
Diabetes Mellitus Tipo 2/fisiopatologia
Modelos Animais de Doenças
Seleção Artificial
Genótipo
Hiperglicemia/dietoterapia
Hiperglicemia/etiologia
Mitocôndrias/enzimologia
Óxido Nítrico/análise
Óxido Nítrico/metabolismo
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950781
Autor: Cid-Hernández, Margarita; Ramírez-Anguiano, Ana C; Ortiz, Genaro G; Morales-Sanchez, Eddic W; González-Ortiz, Luis J; Velasco-Ramírez, Sandra F; Pacheco-Moisés, Fermín P.
Título: Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
Fonte: Biol. Res;48:1-6, 2015. graf.
Idioma: en.
Resumo: BACKGROUND: Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were randomly assigned into control non-treated group and groups that received 1,1.5 and 2 g/Kg body weight of dry powder of Kh fruit, respectively. Rats were euthanized at day 1 and 7 after treatment. RESULTS: Rats under Kh treatment at all dose levels tested, does not developed any neurologic symptoms. However, we detected alterations in membrane fluidity and ATPase activity. Lower dose of Kh on day 1 after treatment induced higher mitochondrial membrane fluidity than control group. This change was strongly correlated with increased ATPase activity and pH gradient driven by ATP hydrolysis. On the other hand, membrane fluidity was hardly affected on day 7 after treatment with Kh. Surprisingly, the pH gradient driven by ATPase activity was significantly higher than controls despite an diminution of the hydrolytic activity of ATPase. CONCLUSIONS: The changes in ATPase activity and pH gradient driven by ATPase activity suggest an adaptive condition whereby the fluidity of the membrane is altered.
Descritores: Mitocôndrias Hepáticas/efeitos dos fármacos
Adenosina Trifosfatases/metabolismo
Karwinskia/toxicidade
Fluidez de Membrana/efeitos dos fármacos
-Frações Subcelulares/efeitos dos fármacos
Partículas Submitocôndricas/efeitos dos fármacos
Mitocôndrias Hepáticas/enzimologia
Distribuição Aleatória
Ratos Sprague-Dawley
Força Próton-Motriz/efeitos dos fármacos
Frutas/toxicidade
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


  3 / 94 LILACS  
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Id: biblio-973506
Autor: Chies, Agnaldo Bruno; Nakazato, Paula Carolina Grande; Spadella, Maria Angélica; Zorzi, Patrícia; Gomes, Maria Cecília Jordani; D'Albuquerque, Luiz Augusto Carneiro; Castro-e-Silva, Orlando.
Título: Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
Fonte: Acta cir. bras;33(9):775-784, Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate whether pre-treatment with rivastigmine is able to attenuate the I/R induced lesions in rat liver. Methods: SHAM animals or those submitted to I/R, non-treated or pre-treated with rivastigminine (2mg/kg) either 50 or 15 minutes before ischemia, were used. After I/R protocol, these animals were killed and their livers were harvested to measurement of the mitochondrial swelling as well as the malondialdehyde (MDA), nitrite and nitrate tissue concentration. Blood was also harvested for serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) determinations. Results: I/R promoted a significant increase of mitochondrial swelling in the studied animals. This increase of mitochondrial swelling was partially prevented by rivastigmine, but only if administered 50 minutes before ischemia. No significant modification of MDA, nitrite or nitrate tissue concentrations was observed in consequence of I/R, followed or not by rivastigmine treatments. In addition, I/R elevated both AST and ALT. These elevations of serum enzymes were not reversed by the different rivastigmine treatments. Conclusions: Rivastigmine administered 50 minutes before ischemia attenuates I/R-induced mitochondrial swelling, that indicates liver injury. This protective effect may be related to a greater stimulation of α7nAChR present in the Kupffer cells by the non-methabolized ACh, leading to an attenuation of I/R-induced inflammation.
Descritores: Traumatismo por Reperfusão/prevenção & controle
Rivastigmina/administração & dosagem
Isquemia/complicações
Fígado/irrigação sanguínea
-Aspartato Aminotransferases/sangue
Mitocôndrias Hepáticas
Traumatismo por Reperfusão/patologia
Ratos Wistar
Miopatias Mitocondriais/prevenção & controle
Alanina Transaminase/sangue
Modelos Animais de Doenças
Isquemia/sangue
Fígado/efeitos dos fármacos
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-973484
Autor: Castro-e-Silva, Orlando; D'Albuquerque, Luiz Augusto Carneiro; Silveira, Marina Rodrigues Garcia; Zorzi, Patricia; Liu, Jordan Bistafa; Campos, Daniel Tófoli Queiroz; Victorino, João Paulo; Jordani, Maria Cecília; Mendes, Karina Dal Sasso; Évora, Paulo Roberto Barbosa.
Título: Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion
Fonte: Acta cir. bras;33(12):1043-1051, Dec. 2018. graf.
Idioma: en.
Resumo: Abstract Purpose: To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process. Methods: Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups: 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondrial function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%. Results: State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondrial osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB. Conclusions: The methylene blue was not able to restore the mitochondrial parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species.
Descritores: Traumatismo por Reperfusão/prevenção & controle
Inibidores Enzimáticos/uso terapêutico
Fígado/irrigação sanguínea
Azul de Metileno/uso terapêutico
-Consumo de Oxigênio
Aspartato Aminotransferases/sangue
Valores de Referência
Fatores de Tempo
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Traumatismo por Reperfusão/metabolismo
Reprodutibilidade dos Testes
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Ratos Wistar
Respiração Celular
Alanina Transaminase/sangue
Inibidores Enzimáticos/farmacologia
Membranas Mitocondriais/efeitos dos fármacos
Membranas Mitocondriais/metabolismo
Fígado/metabolismo
Malondialdeído/análise
Azul de Metileno/farmacologia
Dilatação Mitocondrial/efeitos dos fármacos
Limites: Humanos
Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-949372
Autor: Nakazato, Paula Carolina Grande; Victorino, João Paulo; Fina, Clarice Fleury; Mendes, Karina Dal Sasso; Gomes, Maria Cecília Jordani; Evora, Paulo Roberto Barbosa; D'Albuquerque, Luiz Augusto Carneiro; Castro-e-Silva, Orlando.
Título: Liver ischemia and reperfusion injury. Pathophysiology and new horizons in preconditioning and therapy
Fonte: Acta cir. bras;33(8):723-735, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract It is well known that during hepatic operative procedures, it is often critical that the irrigation is interrupted to avoid possible bleeding, blood transfusions, variable intensities, and their short and long-term consequences. It was believed in the past that the flow interruption should not exceed 20 minutes, which limited the use of this maneuver. However, it has been postulated that ischemia could be maintained for more than 60 minutes in healthy livers. The present paper review includes: 1) A brief introduction to justify the rationale of the review design; 2) Aspects of the pathophysiology of the three stages of the liver ischemia-reperfusion injury; 3) The innate and acquired immunity; 4) Oxidative stress; 5) Apoptosis and autophagy, Some essential biomarkers (Tumor Necrosis Factor-α, nitric oxide, metalloproteinases); and, finally; 6) Preventive ("cheating") strategies, non-pharmacological and pharmacological options to treat the liver IR injury.
Descritores: Traumatismo por Reperfusão/fisiopatologia
Traumatismo por Reperfusão/terapia
Precondicionamento Isquêmico/métodos
Isquemia/fisiopatologia
Isquemia/terapia
Fígado/irrigação sanguínea
-Fatores de Tempo
Mitocôndrias Hepáticas/metabolismo
Traumatismo por Reperfusão/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Morte Celular/fisiologia
Estresse Oxidativo/fisiologia
Metaloproteinases da Matriz/metabolismo
Isquemia/metabolismo
Óxido Nítrico/metabolismo
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-779759
Autor: Mumic, Fabricia Teixeira; Silveira, Marina Rodrigues Garcia da; Vilalva, Kelvin Henrique; Jordani, Maria Eliza; Gomes, Maria Cecilia Jordani; Vanni, José Carlos; Vollet Filho, José Dirceu; Kurachi, Cristina; Bagnato, Vanderlei Salvador; Silva, Orlando de Castro e.
Título: Effect of irradiation with different laser wavelengths on oxidative stress of non-hepatectomized rats
Fonte: Acta cir. bras;31(supl.1):40-44, 2016. graf.
Idioma: en.
Resumo: PURPOSE: To assess the effect of two laser wavelengths, either separate or combined, on intact rat livers. METHOD: Nineteen male Wistar rats (200-300 g) were submitted to laser irradiation at 5 different sites on the liver surface.Wavelengths 660 and 780 nm were used, with a dose of irradiation of 60 J/cm2/site.The animals were divided into the groups:control (C) and animals irradiated with 660 nm laser (L1), with 780 nm laser (L2) or withboth wavelengths (L3).Mitochondrial function, mitochondrial swelling, and hepatocellular malondialdehyde (MDA) levels were determined.Data were analyzed by the Mann-Whitney test, with the level of significance set at 5%. RESULTS: There was a reduction of ADP-activated respiration (state 3) in group L1 compared to group C (p=0.0016), whereas the values of group L2 were similar to control.Group L3 also showed a reduction of state 3 (p=0.0159).There was a reduction of RCR in group L1 compared to control (p=0.0001) and to group L2 (p=0.0040).Mitochondrial swelling only differed between group L3 and control (p=0.0286).There was a increase in MDA levels in group L3 compared to control (p=0.0476) and to group L2 (p=0.0286) and in group L1 compared to group L2 (p=0.0132). CONCLUSION: Although laser irradiation reduced mitochondrial function,it did not interfere with the hepatocellular energy status.
Descritores: Mitocôndrias Hepáticas/efeitos da radiação
Estresse Oxidativo/efeitos da radiação
Lasers Semicondutores
Fígado/efeitos da radiação
-Doses de Radiação
Espectrofotometria
Fatores de Tempo
Ratos Wistar
Terapia com Luz de Baixa Intensidade
Relação Dose-Resposta à Radiação
Malondialdeído/análise
Dilatação Mitocondrial/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  7 / 94 LILACS  
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Chaib, Eleazar
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Id: lil-767602
Autor: Cavalcante, Fernanda Paula; Coelho, Ana Maria Mendonça; Machado, Marcel Cerqueira Cesar; Sampietre, Sandra Nassa; Patzina, Rosely Antunes; Diniz, Márcio Augusto; Chaib, Eleazar; D'Albuquerque, Luiz Augusto Carneiro.
Título: Mechanisms of the beneficial effect of sevoflurane in liver ischemia/reperfusion injury
Fonte: Acta cir. bras;30(11):749-755, Nov. 2015. tab, graf.
Idioma: en.
Resumo: PURPOSE: To evaluate the underlying mechanisms by which sevoflurane protects the liver against ischemia/reperfusion injury evaluate the mechanism by which sevoflurane exerts this protective effect. METHODS: Twenty-six rats were subjected to partial ischemia/reperfusion injury for 1h: one group received no treatment, one group received sevoflurane, and sham group of animals received laparotomy only. Four hours after reperfusion, levels of alanine and aspartate aminotransferases, tumor necrosis factor-a, and interleukins 6 and 10 were measured. Analyses of mitochondrial oxidation and phosphorylation, malondialdehyde content, histology, and pulmonary vascular permeability were performed. RESULTS: Serum levels of alanine and aspartate aminotransferases were significantly lower in the sevoflurane group compared to untreated controls (p<0.05). The sevoflurane group also showed preservation of liver mitochondrial function compared to untreated controls (p<0.05). Sevoflurane administration did not alter increases in serum levels of tumor necrosis factor-a, and interleukins 6 and 10. Sevoflurane treatment significantly reduced the coagulative necrosis induced by ischemia/reperfusion (p<0.05). Pulmonary vascular permeability was preserved in the sevoflurane group compared to untreated controls. CONCLUSION: Sevoflurane administration protects the liver against ischemia/reperfusion injury, via preservation of mitochondrial function, and also preserves lung vascular permeability.
Descritores: Anestésicos Inalatórios/farmacologia
Isquemia/prevenção & controle
Fígado/irrigação sanguínea
Éteres Metílicos/farmacologia
Mitocôndrias Hepáticas/efeitos dos fármacos
Traumatismo por Reperfusão/prevenção & controle
-Alanina Transaminase/sangue
Aspartato Aminotransferases/sangue
Permeabilidade Capilar/efeitos dos fármacos
Citocinas/sangue
Isquemia/patologia
Peroxidação de Lipídeos
Fígado/patologia
Mitocôndrias Hepáticas/fisiologia
Necrose
Fosforilação
Ratos Wistar
Reprodutibilidade dos Testes
Traumatismo por Reperfusão/patologia
Fatores de Tempo
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Texto completo SciELO Saúde Pública
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Id: lil-733306
Autor: Rivera-Ávila, Roberto Carlos.
Título: Fiebre chikungunya en México: caso confirmado y apuntes para la respuesta epidemiológica / Chikungunya fever in Mexico: confirmed case and notes on the epidemiologic response
Fonte: Salud pública Méx;56(4):402-404, jul.-ago. 2014. tab.
Idioma: es.
Resumo: La fiebre chikungunya (CHIK) es una enfermedad viral transmitida al ser humano por el mismo vector del dengue, el mosquito Aedes. Además de fiebre y fuertes dolores articulares, produce otros síntomas como mialgias, cefalea, náuseas, cansancio y exantema. No tiene tratamiento específico; el manejo terapéutico de los pacientes se enfoca en el alivio de los síntomas. Históricamente se han reportado brotes de grandes proporciones; incluso desde 2010 se llegó a considerar como una potencial epidemia emergente. En 2013 se introdujo a las islas del Caribe y recientemente se ha reportado en el continente americano. En este trabajo se describe el primer caso confirmado de chikungunya en México, en el municipio de Tlajomulco de Zúñiga, Jalisco, en mayo de 2014, importado de la isla Antigua y Barbuda, en el Caribe, por una mujer de 39 años de edad.

Chikungunya fever (CHIK) is a viral disease transmitted to human beings by the same vector as dengue -the Aedes mosquito. Besides fever and severe pain in the joints, it produces other symptoms such as myalgias, headache, nausea, fatigue and exanthema. There is no specific treatment for it; the therapeutic management of patients focuses on symptom relief. Historically, outbreaks of large proportions have been reported; even since 2010 it was considered to be a potential emerging epidemic. In 2013 it was introduced into the islands of the Caribbean, and it has recently been reported in the American continent. This paper describes the first confirmed case of chikungunya in Mexico -in the municipality of Tlajomulco de Zúñiga, Jalisco, in May, 2014-, which was imported from the Caribbean island of Antigua and Barbuda by a 39 year-old woman.
Descritores: Antídotos/farmacologia
Temperatura Alta
Imidazóis/toxicidade
Carne
Mitocôndrias/metabolismo
Mutagênicos/toxicidade
Consumo de Oxigênio/efeitos dos fármacos
Ubiquinona/farmacologia
-Antídotos/administração & dosagem
Culinária
Dieta
Complexo II de Transporte de Elétrons
Complexo III da Cadeia de Transporte de Elétrons/metabolismo
Complexo IV da Cadeia de Transporte de Elétrons/metabolismo
Transporte de Elétrons/efeitos dos fármacos
Alimentos Fortificados
Mitocôndrias Cardíacas/efeitos dos fármacos
Mitocôndrias Cardíacas/metabolismo
Mitocôndrias Hepáticas/efeitos dos fármacos
Mitocôndrias Hepáticas/metabolismo
Mitocôndrias Musculares/efeitos dos fármacos
Mitocôndrias Musculares/metabolismo
Complexos Multienzimáticos/metabolismo
NAD(P)H Desidrogenase (Quinona)/metabolismo
Oxirredutases/metabolismo
Ratos Wistar
Succinato Desidrogenase/metabolismo
Ubiquinona/administração & dosagem
Limites: Animais
Bovinos
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-732253
Autor: Sáinz-Pardo, Marta; Estevez, Roberto; Pablo, Óliver Valencia de; Rossi-Fedele, Giampiero; Cisneros, Rafael.
Título: Root Canal Penetration of a Sodium Hypochlorite Mixture Using Sonic or Ultrasonic Activation
Fonte: Braz. dent. j;25(6):489-493, Nov-Dec/2014. tab, graf.
Idioma: en.
Resumo: The purpose of this ex vivo study was to determine, in "open" and "closed" systems, whether the design has an influence on the penetration length of sodium hypochlorite mixed with a radiopaque contrast medium, measured in millimeters, when delivered using positive pressure (PP) and using sonic (SI) or passive ultrasonic (PUI) activation. Sixty single-rooted teeth were divided into two groups: open and closed systems (n=30). Root canal shaping was performed to a working length of 17 mm. The samples were divided into three sub-groups (n=10) according to irrigant delivery and activation: PP, and SI or PUI activation. By using radiographs, penetration length was measured, and vapor lock was assessed. For the closed group, the penetration distance means were: PP 15.715 (±0.898) mm, SI 16.299 (±0.738) mm and PUI 16.813 (±0.465) mm, with vapor lock occurring in 53.3% of the specimens. In the open group, penetration to 17 mm occurred in 97.6% of the samples, and no vapor lock occurred. Irrigant penetration and distribution evaluation using open and closed systems provide significantly different results. For closed systems, PUI is the most effective in delivering the irrigant to working length, followed by SI.

O objetivo deste estudo in vivo foi determinar, para os sistemas "abertos" e "fechados", se o design tem influência na penetração, em milímetros, do hipoclorito de sódio misturado com um meio radiopaco quando empregado na ativação com pressão positiva (PP) e ativação sônica (SI) ou ultrassônica passiva (PUI). Sessenta dentes unirradiculares foram divididos em dois grupos: sistema aberto e sistema fechado (n=30). Os canais radiculares foram trabalhados até um comprimento de trabalho de 17 mm. Os grupos foram subdivididos em três subgrupos (n=10) de acordo com a solução irrigadora e a ativação: PP, e ativação SI ou PUI. Usando radiografias, a distância de penetração foi medida e avaliado o vapor contido. Para o grupo fechado, as distâncias médias de penetração foram PP 15,715 (±0,898) mm, SI 16,299 (±0,738) mm e PUI 16,813 (±0,465) mm e houve vapor contido em 53,3% das amostras. No grupo aberto, houve penetração de 17 mm em 97.6% das amostras, sem contenção de vapor. A penetração do irrigante e avaliação da distribuição usando sistemas aberto e fechado produziram resultados significativamente diferentes. Para os sistemas fechados, PUI é mais eficaz para levar o irrigante até preencher o comprimento de trabalho, seguido por SI.
Descritores: Ubiquinona/metabolismo
-/análogos & derivados
ABBREVIATIONS AS TOPIC-CHLORO-ABDOMINAL INJURIES-NITROBENZOFURAZAN/análogos & derivados
/síntese química
ABBREVIATIONS AS TOPIC-CHLORO-ABDOMINAL INJURIES-NITROBENZOFURAZAN/síntese química
Difusão
Transporte de Elétrons
Corantes Fluorescentes
Lipossomos
Camundongos Endogâmicos ICR
Mitocôndrias Hepáticas/metabolismo
Fosfatidiletanolaminas
Ubiquinona/análogos & derivados
Ubiquinona/biossíntese
Ubiquinona/síntese química
Limites: Animais
Camundongos
Tipo de Publ: Research Support, U.S. Gov't, P.H.S.
Responsável: BR1.1 - BIREME


  10 / 94 LILACS  
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Biondo, Alexander Welker
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Id: lil-731249
Autor: Santos, Andrea Pires dos; Conrado, Francisco de Oliveira; Messick, Joanne Belle; Biondo, Alexander Welker; Oliveira, Simone Tostes de; Guimaraes, Ana Marcia Sá; Nascimento, Naíla Cannes do; Pedralli, Viviane; Lasta, Camila Serina; González, Félix Hilário Diaz.
Título: Hemoplasma prevalence and hematological abnormalities associated with infection in three different cat populations from Southern Brazil / Prevalência da infecção por hemoplasmas e alterações hematológicas associadas à infecção em três diferentes populações de gatos do sul do Brasil
Fonte: Rev. bras. parasitol. vet;23(4):428-434, Oct-Dec/2014. tab, graf.
Idioma: en.
Resumo: Three hemoplasma species are recognized in domestic cats: Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’ and ‘Candidatus Mycoplasma turicensis’. We report the prevalence and hematological abnormalities of hemoplasma infection in 369 domestic cats from three different populations (blood donors, hospitalized cats and shelter cats) from Southern Brazil. Complete blood counts were performed at the time of blood collection, and DNA was extracted and tested by conventional PCR for each hemoplasma species. A total of 79 samples (21.40%) were positive for at least one species. The most prevalent hemoplasma was ‘Candidatus Mycoplasma haemominutum’, with 50/369 (13.55%) positive cats, followed by ‘Candidatus Mycoplasma turicensis’, 10/369 (2.71%), and Mycoplasma haemofelis, 8/369 (2.16%). Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ coinfection was observed in 4/369 (1.08%), whereas ‘Candidatus Mycoplasma haemominutum’ and ‘Candidatus Mycoplasma turicensis’ in 5/369 (1.35%). Three cats (0.81%) were infected with all three hemoplasmas. There was no association between infection and the different populations. Anemia was associated with Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’, but not with ‘Candidatus Mycoplasma turicensis’. Male cats and cats with outdoor access were more likely to be infected. Although ‘Candidatus Mycoplasma haemominutum’ is believed to cause minimal or no hematological alterations, the infected cats studied herein were more likely to be anemic.

Três espécies de hemoplasmas são reconhecidas em gatos domésticos: Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’ e ‘Candidatus Mycoplasma turicensis’. A prevalência e alterações hematológicas associadas à infecção por hemoplasmas foi estudada, em 369 gatos domésticos de três populações distintas (doadores de sangue, hospitais e gatos de abrigo) do Sul do Brasil. Foram realizados hemogramas completos no momento da coleta de sangue e as amostras tiveram seu DNA extraído e testado por PCR convencional para cada espécie de hemoplasmas. Setenta e nove amostras (21,40%) foram positivas para pelo menos uma espécie. O mais prevalente foi ‘Candidatus Mycoplasma haemominutum’ com 50/369 (13,55%) gatos positivos, seguidos por ‘Candidatus Mycoplasma turicensis’ com 10/369 (2,71%) e Mycoplasma haemofelis com 8/369 (2,16%). Coinfecção por Mycoplasma haemofelis e ‘Candidatus Mycoplasma haemominutum’ foi observada em 4/369 (1,08%), enquanto ‘Candidatus Mycoplasma haemominutum’ e ‘Candidatus Mycoplasma turicensis’ coinfectaram 5/369 (1,35%) gatos. Três (0,81%) gatos apresentaram infecção pelos três hemoplasmas. Não houve associação entre a infecção e as diferentes populações. Anemia foi associada com a infecção por Mycoplasma haemofelis e ‘Candidatus Mycoplasma haemominutum’, mas não com ‘Candidatus Mycoplasma turicensis’. Gatos machos e com acesso à rua apresentaram maior probabilidade de serem infectados. Embora se acredite que ‘Candidatus Mycoplasma haemominutum’ possa causar alterações hematológicas mínimas ou ausentes, gatos infectados encontrados neste estudo foram mais propensos à anemia.
Descritores: Hepatócitos/efeitos dos fármacos
Mitocôndrias Hepáticas/efeitos dos fármacos
NAD(P)H Desidrogenase (Quinona)/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Ubiquinona/farmacologia
-Células Cultivadas
Citoproteção
Membrana Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Glutationa/metabolismo
Hepatócitos/enzimologia
Potenciais da Membrana/efeitos dos fármacos
Mitocôndrias Hepáticas/enzimologia
NAD
Oxirredução
Ratos Sprague-Dawley
Espécies Reativas de Oxigênio/metabolismo
Rotenona/toxicidade
Desacopladores/toxicidade
/farmacologia
VITAMIN K ABATTOIRS/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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