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Pesquisa : A11.284.430.214.190.875.811 [Categoria DeCS]
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Id: biblio-964052
Autor: Méndez-López, T T; Rodríguez-Orozco, A R; Béjar Lozano, C.
Título: Efecto comparado de dosis inmunoestimulantes de fracciones ribosomales y proteoglicanos de membrana sobre la activación de subpoblaciones de células mononucleadas humanas de sangre periférica / Compared effect of immunostimulatory dose of ribosomal fractions and membrane proteoglycans on the activation of human mononuclear cell subsets from peripheral blood
Fonte: Arch. alerg. inmunol. clin;43(1):10-14, 2012.
Idioma: es.
Resumo: Antecedentes. La población mexicana presenta una alta prevalencia de infecciones recurrentes de vías respiratorias altas. Objetivos. Comparar el efecto de dosis inmunoestimulantes de ribosomas bacterianos y proteoglicanos de membrana Ribovac® sobre células mononucleadas. Material y métodos. La expresión de IL-6 de células mononucleadas en cultivo, se midió a concentraciones y tiempos variables por la técnica de ELISA, mientras que el efecto de Ribovac® en poblaciones de células mononucleadas fue analizado por citometría de flujo. Resultados. Ribovac® tiene un efecto dependiente de dosis y tiempo de exposición sobre la expresión de IL-6 por células mononucleadas; las concentraciones de IL-6 fueron máximas a las 6 horas de tratamiento con Ribovac®. La expresión de CD3+ fue mayor cuando las células mononucleadas se trataron con 125 µg/ml por 72 horas (p=0,010) respecto a aquellas que se trataron a mitad de esa concentración en igual tiempo, a diferencia de la expresión de CD19, que fue mayor en células mononucleadas tratadas con 62,5 µg/ml por 72 horas que en aquellas tratadas con 125 µg/ml por 72 horas (p=0,021). No se encontraron disminuciones estadísticamente significativas en el número de células CD16+CD56+ ni en la coexpresión de los marcadores CD45 y CD19 cuando se compararon tanto tiempos de administración como concentraciones de Ribovac®. Conclusiones. La expansión de poblaciones linfoides y la maduración de éstas a fenotipos con mayor capacidad efectora son efectos inducibles y deseables de Ribovac®, que deben tenerse en cuenta al decidir su tiempo e intervalos de administración.(AU)

Background. The Mexican population has a high prevalence of recurrent infections of upper respiratory tract. Objective. To compare the effect of immunostimulatory dose of bacterial ribosomes and membrane proteoglycan Ribovac® on mononuclear cells. Methods. The expression of interleukin 6 from mononuclear cells in culture was measured at varying concentrations and times by ELISA, while the effect of R in populations of mononuclear cells was analyzed by flow cytometry. Results. Ribovac® has an dose-dependent and exposure time effect on the expression of IL-6 by mononuclear cells, concentrations of IL-6 were maximal at 6 hours of treatment with Ribovac®. The expression of CD3+ was higher when mononuclear cells weretreated with 125 µg/ml for 72 hours (p=0,010) than those who were treated to half that concentration in the same time, unlike the expression of CD19 which was higher in mononuclear cells treated with 62,5 µg/ml for 72 hours than those that were treated with 125 µg/ml for 72 hours (p=0,021). There were no statistically significance in the decrease in the number of CD16+CD56+ cells and in the coexpression of CD45 and CD19 markers neither; when comparing both times of administration and evaluated concentrations of Ribovac®. Conclusions. The lymphoid population expansion and their maturation to better effector phenotypes effector are inducible and desirable effects of Ribovac® and these important when deciding the time and intervals of administration.(AU)
Descritores: Proteoglicanas
Ribossomos
Adjuvantes Imunológicos
-Infecções Respiratórias
Leucócitos Mononucleares
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Texto completo SciELO Brasil
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Id: lil-623779
Autor: Normier, G; Pinel, A. M; Domzig, W; Dussourd D'Hinterland, L.
Título: Immunomodulation by microbial ribosomes
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.2):163-172, 1987. graf, ilus.
Idioma: en.
Conferência: Apresentado em: International Symposium on Immunomodulators: Biology and Therapeutic Applications, Rio de Janeiro, Apr. 26-30, 1987.
Resumo: Over the past twenty years, many authors have reported evidence of the immunoprotective capacity of ribosomes isolated from bacteria, fungi and parasites. Since 1971 we have explored the protective capacity of ribosomes isolated from a large variety of microorganisms responsible for human and animal diseases. More recently, using monoclonal antibodies raised against ribosomes and then selected for their ability to confer passive immunity to mice, we have studied the mechanism of the protection induced by ribosomes. These studies, in parallel with the development of a technology for the large scale production of ribosomes, have allowed us to achieve a new regard for ribosomal vaccines for use in human. The general concept of ribosomal vaccines in presented and examples of two such vaccines are described with data on the specific protection that they induce in mice against experimental infections with Klebsiella peneumoniae, Streptococcus pneumoniae, S. pyogenes and Haemophilus influenzae for the first one, and against Candida albicans type A and type B for the second one. Because of their high immunogenicity and their innocuity these vaccines represent a decisive improvement over classical microbial vaccines.
Descritores: Ribossomos/genética
Rodopsinas Microbianas/uso terapêutico
Imunomodulação/genética
-Fatores Imunológicos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-742970
Autor: Cruz, Lucinéia Claudia de Toni Aquino da; Serra, Otacília Pereira; Leal-Santos, Fábio Alexandre; Ribeiro, Ana Lucia Maria; Slhessarenko, Renata Dezengrini; Santos, Marina Atanaka dos.
Título: Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;48(1):18-25, jan-feb/2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION: Dengue is the most prevalent arboviral disease in tropical areas. In Mato Grosso, outbreaks are reported every year, but studies on dengue in this state are scarce. METHODS: Natural transovarial infection of Aedes aegypti by a flavivirus was investigated in the Jardim Industriário neighborhood of Cuiabá, Mato Grosso. Eggs were collected with ovitraps during the dry, intermediate, and rainy seasons of 2012. After the eggs hatched and the larvae developed to adulthood, mosquitoes (n = 758) were identified and allocated to pools of 1-10 specimens according to the collection location, sex, and climatic period. After RNA extraction, multiplex semi-nested RT-PCR was performed to detect the four dengue virus (DENV) serotypes, yellow fever virus, West Nile virus and Saint Louis encephalitis virus. RESULTS: DENV-4 was the only flavivirus detected, and it was found in 8/50 pools (16.0%). Three of the positive pools contained females, and five contained males. Their nucleotide sequences presented 96-100% similarity with DENV-4 genotype II strains from Manaus, Amazonas. The minimum infection rate was 10.5 per 1000 specimens, and the maximum likelihood estimator of the infection rate was 11.6 (95% confidence interval: 4.8; 23.3). CONCLUSIONS: This study provides the first evidence of natural transovarial infection by DENV-4 in Ae. Aegypti in Mato Grosso, suggesting that this type of infection might serve as a mechanism of virus maintenance during interepidemic periods in Cuiabá, a city where dengue epidemics are reported every year. These results emphasize the need for efficient vector population control measures to prevent arbovirus outbreaks in the state. .
Descritores: Cinesina/metabolismo
Biossíntese de Proteínas
-Linhagem Celular
Centrifugação com Gradiente de Concentração
Técnicas de Silenciamento de Genes
Imunoprecipitação
Interfase
Cinesina/antagonistas & inibidores
Cinesina/genética
Microtúbulos/metabolismo
Iniciação Traducional da Cadeia Peptídica
Ligação Proteica
Pirimidinas/farmacologia
Interferência de RNA
Ribossomos/metabolismo
Tionas/farmacologia
Limites: Animais
Humanos
Camundongos
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: BR1.1 - BIREME


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Id: lil-721069
Autor: Calich, Ana Luisa Garcia.
Título: Anticorpo antiproteína P ribossomal em pacientes com hepatite autoimune / Anti-ribosomal P protein antibody in autoimmune hepatitis patients.
Fonte: São Paulo; s.n; 2013. [62] p. tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: Introdução: Os anticorpos antiproteína P ribossomal (anti-P) são considerados marcadores sorológicos específicos do Lúpus Eritematoso Sistêmico (LES) e estão associados a acometimento hepático nesta doença. As semelhanças entre a hepatite autoimune (HAI) e a hepatite associada ao LES levou ao questionamento se o anticorpo anti-P também estaria presente na HAI. Objetivo: Avaliar a frequência e significância clínica do anticorpo anti-P em uma grande coorte de pacientes com HAI. Métodos: Foram analisados os soros de 96 pacientes com HAI, coletados no diagnóstico e comparados com 82 soros de indivíduos saudáveis. Todos os soros foram testados para a presença do anticorpo anti-P pelo método de ELISA, do anticorpo anti-DNA de dupla fita pelo método de imunofluorescência indireta usando Crithidia luciliae e do anticorpo anti-Sm pelo método de ELISA. Os critérios de exclusão adotados foram a presença de outros anticorpos específicos de LES como o anti-DNA de dupla fita (n=1) e o anti-Sm (n=2) ou se o paciente apresentasse o diagnóstico de LES definido pelo Colégio Americano de Reumatologia (n=0). Os prontuários médicos foram revisados para dados demográficos, clínicos e resultados de exames laboratoriais relacionados a hepatopatia e anticorpos específicos de HAI. Resultado: Títulos moderados ou alto (> 40 U) de anti-P foram encontrados em 9,7% (9/93) dos pacientes com HAI e em nenhum dos controles (p = 0,003). No diagnóstico, os pacientes com anti-P positivo ou negativo apresentavam características demográficas/clínicas semelhantes, como a frequência de cirrose (44,4% vs 28,5%, p = 0,44) e exames laboratoriais relacionados a hepatite (p > 0,05). Entretanto, ao final do seguimento destes pacientes (média de 10,2 ± 4,9 anos), os pacientes positivos para anticorpos anti-P apresentaram uma maior frequência de cirrose quando comparados a pacientes negativos para anti-P (100% vs 60%, p = 0,04). Conclusão: a demonstração da presença do anticorpo anti-P em pacientes com HAI...

Background: Autoantibodies to ribosomal P proteins (anti-rib P) are specific serological markers for systemic lupus erythematosus (SLE) and are associated with liver involvement in this disease. The similarity in autoimmune background between autoimmune hepatitis (AIH) and SLE- associated hepatitis raises the possibility that anti-rib P antibodies might also have relevance in AIH. Aims: To evaluate the frequency and clinical significance of anti-rib P antibodies in a large AIH cohort. Methods: Sera obtained at diagnosis of 96 AIH patients and of 82 healthy controls were tested for IgG anti-ribosomal P protein by ELISA. All of the sera were also screened for other lupus-specific autoantibodies, three patients with the presence of anti-dsDNA (n=1) and anti-Sm (n = 2) were excluded. Results: Moderate to high titers (> 40 U) of anti-rib P antibody were found in 9.7% (9/93) of the AIH patients and none of the controls (P = 0.003). At presentation, AIH patients with and without anti-rib P antibodies had similar demographic/clinical features, including the frequency of cirrhosis (44.4% vs. 28.5%, P = 0.44), hepatic laboratorial findings (p > 0.05). Importantly, at the final observation (follow-up period 10.2 ± 4.9 years), the AIH patients with anti-rib P had a significantly higher frequency of cirrhosis compared to the negative group (100% vs. 60%, P = 0.04). Conclusion: The novel demonstration of anti-rib P in AIH patients without clinical or laboratory evidence of SLE suggests a common underlying mechanism targeting the liver in these two diseases. In addition, this antibody appears to predict the patients with worse AIH prognoses...
Descritores: Anticorpos
Hepatite Autoimune
Lúpus Eritematoso Sistêmico
Prognóstico
Ribossomos
Limites: Humanos
Feminino
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


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Id: lil-644222
Autor: Akamatsu, FE; Gama, EF; Andrade, M; De Souza, RR; Jacomo, AL.
Título: Effect of prenatal low-protein diet in subepicardial neuron of rat: a morphological study
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;27(2):82-87, Apr.-June 2010. ilus.
Idioma: en.
Resumo: Heart autonomic ganglia are known to play an important role in cardiac rhythm control, protecting againstcertain arrhythmias due to their parasympathetic activity. Cardiac disorders may arise following starvingstates during pregnancy; cardiac performance and cardiac fibers have been shown to suffer deleterious effectsunder starvation. Morphology of these plexuses may suffer interference of extrinsic factors, but data is stilllacking about the effects of low protein diet during pregnancy and early postnatal period on subepicardialneuron structure. Two groups of pregnant Wistar rats were submitted to different diets according to itsprotein content, normal and 5% casein, until 21 days after delivery. The offspring was divided in two groups,D and N, according to their motherLs diet, low and normal protein respectively, and then sacrificed. Theatrial neurons were identified by ƒÀ-nicotinamide adenine dinucleotide (NADH) and adenine dinucleotidephosphate diaphorase (NADPH-d) staining. Profile areas of the nerve cell bodies were measured. NADHstaining did not show significant differences between groups but NADPH- d profile areas of nerve cell bodiesfrom group D were smaller than in control group. Ultrastructural changes were observed in group D rats:agglomerated ribosomes, increase in nucleoli density and irregular chromatin.Low-protein diet in rats at earlydevelopmental stages interferes in size, and ultrastructure of subepicardial neurons. Even though underfeedingduring perinatal period did not produce neuronal death, neuron development is delayed and permanentchanges can supervene in long term.
Descritores: Arritmias Cardíacas
Dieta com Restrição de Proteínas
Gânglios
Desnutrição
Neurônios
Ribossomos
-Desnutrição/dietoterapia
Ratos Wistar
Limites: Animais
Feminino
Gravidez
Ratos
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL


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Id: lil-644141
Autor: Akamatsu, F E; Gama, E F; Andrade, M; De Souza, R R; Jacomo, Al.
Título: Effects of protein deprivation and refeeding on the subepicardial neurons of rat: subepicardial neurons and low-protein diet
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;28(2):120-128, Apr.-June 2011. tab, ilus.
Idioma: en.
Resumo: Heart autonomic ganglia play an important role in cardiac rhythm control, protecting against certainarrhythmias due to their parasympathetic activity. Starvation during pregnancy may cause cardiac disorders andhinder optimal cardiac performance. Also, morphology of subepicardial neuron is subjected to the influenceof extrinsic factors. We studied the influence of protein deprivation on subepicardic neurons in rats at earlydevelopment stages and the effect of restoration of a normal diet.: Three groups of pregnant Wistar rats weresubmitted to different diets according to its protein content: normal (NN group) and 5% casein (DD group),until 42 days after delivery and low protein for 21 days with refeeding for a further 21 days (RN group).All animal were weighed. The number and area of neuronal profiles were measured. The neurons werestained by histochemical methods â-nicotinamide adenine dinucleotide (NADH) and â-nicotinamide adeninedinucleotide phosphate diaphorase (NADPH-d) and their ultra structure were observed.Group DD and RNanimals weighed less than those from group NN. The number of neurons and the cellular profile area didnot show significant differences among groups for both techniques. Endoplasmatic reticulum ribosomes inneurons of undernourished animals showed decreased electron density. Protein deprivation in early stages ofdevelopment produces ultra structural changes but does not alter the number and profile area of nerve cellbodies in rats.
Descritores: Retículo Endoplasmático
Dieta
Neurônios
PROTEIN MALNUTRITION
Ribossomos/metabolismo
-Transtornos Nutricionais
Ratos Wistar/anatomia & histologia
Limites: Animais
Feminino
Gravidez
Ratos
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL


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Texto completo SciELO Venezuela
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Id: lil-630577
Autor: Montbrun, Lilia Cruz de.
Título: Monitoreo de la producción celular de proteínas por análisis del perfil de ribosomas (ribosome profiling) / Monitoring protein cellular production by ribosome profiling
Fonte: Gac. méd. Caracas;117(3):225-225, sep. 2009.
Idioma: es.
Descritores: RNA
Ribossomos/genética
Métodos de Análise/métodos
-Genoma
Monitoramento
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Texto completo SciELO Brasil
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Id: lil-607513
Autor: Jiménez, Diego Javier; Montaña, José Salvador; Martínez, María Mercedes.
Título: Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils
Fonte: Braz. j. microbiol;42(3):846-858, July-Sept. 2011. ilus, tab.
Idioma: en.
Resumo: With the purpose of isolating and characterizing free nitrogen fixing bacteria (FNFB) of the genus Azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral pH in different zones of Boyacá-Colombia. Isolations were done in selective free nitrogen Ashby-Sucrose agar obtaining a recovery of 40 percent. Twenty four isolates were evaluated for colony and cellular morphology, pigment production and metabolic activities. Molecular characterization was carried out using amplified ribosomal DNA restriction analysis (ARDRA). After digestion of 16S rDNA Y1-Y3 PCR products (1487pb) with AluI, HpaII and RsaI endonucleases, a polymorphism of 16 percent was obtained. Cluster analysis showed three main groups based on DNA fingerprints. Comparison between ribotypes generated by isolates and in silico restriction of 16S rDNA partial sequences with same restriction enzymes was done with Gen Workbench v.2.2.4 software. Nevertheless, Y1-Y2 PCR products were analysed using BLASTn. Isolate C5T from tomato (Lycopersicon esculentum) grown soils presented the same in silico restriction patterns with A. chroococcum (AY353708) and 99 percent of similarity with the same sequence. Isolate C5CO from cauliflower (Brassica oleracea var. botrytis) grown soils showed black pigmentation in Ashby-Benzoate agar and high similarity (91 percent) with A. nigricans (AB175651) sequence. In this work we demonstrated the utility of molecular techniques and bioinformatics tools as a support to conventional techniques in characterization of the genus Azotobacter from vegetable-grown soils.
Descritores: Ágar/isolamento & purificação
Sequência de Bases
DNA Ribossômico
Genética Microbiana
Técnicas In Vitro
Fixação de Nitrogênio
Reação em Cadeia da Polimerase
Ribossomos/genética
Microbiologia do Solo
-Métodos
Solo
Métodos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Texto completo SciELO Brasil
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Id: lil-583960
Autor: Hett, Anne Kathrin; Nirchio, Mauro; Oliveira, Claudio; Siccha, Zoila Raquel; Rossi, Anna Rita; Sola, Luciana.
Título: Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family
Fonte: Neotrop. ichthyol;9(1):107-112, Mar. 2011. ilus.
Idioma: en.
Resumo: This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA demonstrated that the nucleolus organizer regions are indeed located on the secondary constrictions of chromosome pair number 1. FISH with 5S rDNA revealed that the minor ribosomal genes are located on this same chromosome pair, near the NORs, though signals are closer to the centromeres and of smaller size, compared to those of the major ribosomal gene clusters. This is the first description of co-localization of major and minor ribosomal genes in the family. Data are discussed from a cytotaxonomic and phylogenetic perspective.

Se presenta la primera descripción del cariotipo de Mugil incilis de Venezuela. El complemento cromosómico está compuesto por 48 cromosomas acrocéntricos uniformemente decrecientes en tamaño. Por lo tanto, los homólogos no pueden ser claramente identificados, con excepción de uno de los pares de mayor tamaño, clasificado como número 1, cuyos homólogos poseen una constricción secundaria subcentromérica, y el par de cromosomas número 24, considerablemente más pequeño que los otros. El bandeo-C reveló bloques heterocromáticos en las regiones centroméricas/pericentroméricas de todos los cromosomas, más conspicuas en el cromosoma 1 en el que las señales C-positivas se encuentra localizada precisamente en la constricción secundaria. La tinción con AgNO3 y la Hibridación Fluorescente in situ (FISH) con sonda 45S rDNA revelaron que las regiones organizadoras del nucléolo están ciertamente localizadas sobre la constricción secundaria del cromosoma número 1. FISH con 5S rDNA reveló que los genes ribosomales menores están ubicados en este mismo par cromosómico, en posición proximal a las NORs, aunque cercanas al centrómero y de menor tamaño en comparación con los clúster de genes ribosomales mayores. Ésta es la primera descripción de co-localización de genes ribosomales mayores y menores en la familia Mugilidae. Los datos se discuten bajo perspectivas citotaxonómicas y filogenéticas.
Descritores: Aberrações Cromossômicas
Peixes/classificação
-Genes/genética
Ribossomos/genética
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  10 / 18 LILACS  
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Id: lil-508063
Autor: Goldfeder, Mauricio Barbugiani.
Título: Caracterização funcional da proteína Cwc24p de Saccharomyces cerevisiae / Functional characterization of Cwc24p in Saccharomyces cerevisiae.
Fonte: São PAulo; s.n; 22 set. 2008. [155] p. ilus, graf, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica para obtenção do grau de Doutor.
Resumo: Em eucariotos, a formação das subunidades ribossomais envolve múltiplos fatores, responsáveis pelas etapas de maturação dos rRNAs e por sua associação a proteínas ribossomais. A via de processamento de pré-rRNA é bastante complexa e inclui várias etapas de modificação de nucleotídeos e clivagens endo- e exonucleolíticas. As modificações de nucleotídeos são dirigidas por snoRNPs, formados por snoRNAs e proteínas, que são divididos em duas classes gerais, de box H/ACA (pseudouridilação) e de box C/O (metilação). Dentre os snoRNP de box C/D esta o U3, que embora apresente as sequências características e se associe a proteínas desse grupo de snoRNPs, não dirige metilações no rRNA, mas sim as clivagens iniciais no pré-rRNA 35S. O snoRNA U3 de Saccharomyces cerevisiae é codificado por dois genes que contêm introns, snR17A e snR17B. Embora a via de montagem do snoRNP U3 ainda não tenha sido determinada com precisão, sabe-se que algumas proteínas do core de box C/O ligam-se ao pré-snoRNA U3 co-transcricionalmente, afetando o splicing e o processamento da extremidade 3´ deste snoRNA...
Descritores: DNA
Técnicas In Vitro
Proteínas de Saccharomyces cerevisiae/análise
Proteínas de Saccharomyces cerevisiae/isolamento & purificação
RNA Ribossômico/análise
RNA Ribossômico/isolamento & purificação
Ribossomos/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/genética
-Meios de Cultura
Escherichia coli/citologia
Escherichia coli/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase
Western Blotting/métodos
Western Blotting
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T574.88, G618c



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