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Pesquisa : A11.329.372 [Categoria DeCS]
Referências encontradas : 620 [refinar]
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Id: biblio-1132541
Autor: Yuan, Chao; Yang, Dandan; Ma, Jia; Yang, Jiali; Xue, Jing; Song, Fuyang; Liu, Xiaoming.
Título: Modulation of Wnt/ß-catenin signaling in IL-17A-mediated macrophage polarization of RAW264. 7 cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;53(8):e9488, 2020. tab, graf.
Idioma: en.
Projeto: This work was financially supported by a grant from National Natural Science Foundation of China.
Resumo: Macrophages play pivotal roles in host defense and immune homeostasis, which have two major functional polarization states, the classically activated M1 and the alternatively activated M2. Interleukin (IL)-17A is an immune modulator able to shape macrophage phenotypes. Wnt/β-catenin is a developmental signaling pathway that plays crucial roles in morphogenesis and tissue homeostasis, which has also been recently demonstrated playing roles in immune regulation. A growing amount of evidence suggests that both Wnt and IL-17A signaling are involved in macrophage polarization. However, their interaction in macrophage polarization remains elusive. The aim of present study was to explore impacts of Wnt/β-catenin on IL-17A-mediated macrophage M1/M2 polarization in murine monocyte/macrophage-like cell line RAW264.7. Results revealed that IL-17A activated Wnt/β-catenin signaling and induced macrophage M1 polarization, but inhibited M2 polarization. In contrast, the activation of Wnt/β-catenin signaling led to the inhibition of M1 macrophage polarization but the promotion of M2 polarization. Importantly, the activation of Wnt/β-catenin also showed abilities to inhibit the IL-17A-induced M1 macrophage polarization while diminishing the IL-17A-inhibited M2 polarization. Molecular analysis further uncovered that the JAK/STAT signaling pathway was involved in the interaction of Wnt/β-catenin and IL-17A in the modulation of macrophage polarization. These results suggested that the Wnt/β-catenin signaling modulated IL-17A-altered macrophage polarization in part by regulating the JAK/STAT signaling pathway. This study thus revealed a novel function of Wnt/β-catenin signaling in regulating IL-17A-altered macrophage polarization.
Descritores: Interleucina-17
beta Catenina
-Via de Sinalização Wnt
Ativação de Macrófagos
Macrófagos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  2 / 620 LILACS  
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Id: biblio-1291618
Autor: Bartsch, Ivonne M; Perelmuter, Karen; Bollati-Fogolín, Mariela; Bartsch J, Angelo; Guzmán, Fanny; Marshall, Sergio H.
Título: An in vitro model mimicking the complement system to favor directed phagocytosis of unwanted cells
Fonte: Electron J Biotechnol;49:5-13, Jan. 2021. ilus, tab, graf.
Idioma: en.
Projeto: Agencia Nacional de Investigación y Desarrollo ANID, Chile.
Resumo: BACKGROUND: Opsonization, is the molecular mechanism by which target molecules promote interactions with phagocyte cell surface receptors to remove unwanted cells by induced phagocytosis. We designed an in vitro system to demonstrate that this procedure could be driven to eliminate adipocytes, using peptides mimicking regions of the complement protein C3b to promote opsonization and enhance phagocytosis. Two cell lines were used: (1) THP-1 monocytes differentiated to macrophages, expressing the C3b opsonin receptor CR1 in charge of the removal of unwanted coated complexes; (2) 3T3-L1 fibroblasts differentiated to adipocytes, expressing AQP7, to evaluate the potential of peptides to stimulate opsonization. (3) A co-culture of the two cell lines to demonstrate that phagocytosis could be driven to cell withdrawal with high efficiency and specificity. RESULTS: An array of peptides were designed and chemically synthesized p3691 and p3931 joined bound to the CR1 receptor activating phagocytosis (p < 0.033) while p3727 joined the AQP7 protein (p < 0.001) suggesting that opsonization of adipocytes could occur. In the co-culture system p3980 and p3981 increased lipid uptake to 91.2% and 89.0%, respectively, as an indicator of potential adipocyte phagocytosis. CONCLUSIONS: This in vitro model could help understand the receptor­ligand interaction in the withdrawal of unwanted macromolecules in vivo. The adipocyte-phagocytosis discussed may help to control obesity, since peptides of C3b stimulated the CR1 receptor, promoting opsonisation and phagocytosis of lipidcontaining structures, and recognition of AQP7 in the differentiated adipocytes, favored the phagocytic activity of macrophages, robustly supported by the co-culture strategy.
Descritores: Fagocitose
Proteínas do Sistema Complemento
Adipócitos
-Técnicas In Vitro
Proteínas Opsonizantes
Técnicas de Cocultura
Células Espumosas
Macrófagos
Microscopia de Fluorescência
Responsável: CL1.1 - Biblioteca Central


  3 / 620 LILACS  
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Id: biblio-1285676
Autor: Xia, Zihuan; Wang, Jiancheng; Yang, Songlin; Liu, Cheng; Qin, Shu; Li, Wenbo; Cheng, Yulong; Hu, Huan; Qian, Jin; Liu, Yi; Deng, Chenliang.
Título: Emodin alleviates hypertrophic scar formation by suppressing macrophage polarization and inhibiting the Notch and TGF-β pathways in macrophages
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(8):e11184, 2021. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Hypertrophic scar (HS) formation is a common complication that develops after skin injury; however, there are few effective and specific therapeutic approaches for HS. Emodin has previously been reported to inhibit mechanical stress-induced HS inflammation. Here, we investigated the molecular mechanisms underlying the inhibitory effects of emodin on HS formation. First, we conducted in vitro assays that revealed that emodin inhibited M1 and M2 polarization in rat macrophages. We subsequently established a combined rat model of tail HS and dorsal subcutaneous polyvinyl alcohol (PVA) sponge-induced wounds. Rats were treated with emodin or vehicle (DMEM). Tail scar specimens were harvested at 14, 28, and 42 days post-incision and subjected to H&E staining and Masson's trichrome staining. Histopathological analyses confirmed that emodin attenuated HS formation and fibrosis. Macrophages were separated from wound cells collected from the PVA sponge at 3 and 7 days after implantation. Flow cytometry analysis demonstrated that emodin suppressed in vivo macrophage recruitment and polarization at the wound site. Finally, we explored the molecular mechanisms of emodin in modulating macrophage polarization by evaluating the expression levels of selected effectors of the Notch and TGF-β pathways in macrophages isolated from PVA sponges. Western blot and qPCR assays showed that Notch1, Notch4, Hes1, TGF-β, and Smad3 were downregulated in response to emodin treatment. Taken together, our findings suggested that emodin attenuated HS formation and fibrosis by suppressing macrophage polarization, which is associated with the inhibition of the Notch and TGF-β pathways in macrophages.
Descritores: Emodina/farmacologia
Cicatriz Hipertrófica/patologia
Cicatriz Hipertrófica/tratamento farmacológico
-Transdução de Sinais
Fator de Crescimento Transformador beta
Macrófagos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  4 / 620 LILACS  
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Aguiar, Maria Cássia Ferreira
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Id: biblio-952056
Autor: Lúcio, Priscilla Suassuna Carneiro; Ribeiro, Daniela Cotta; Aguiar, Maria Cássia Ferreira de; Alves, Pollianna Muniz; Nonaka, Cassiano Francisco Weege; Godoy, Gustavo Pina.
Título: Tumor-associated macrophages (TAMs): clinical-pathological parameters in squamous cell carcinomas of the lower lip
Fonte: Braz. oral res. (Online);30(1):e95, 2016. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract The objective of this study was to analyze the presence of tumor-associated macrophage (TAM) subpopulations M1 and M2 in squamous cell carcinoma of the lower lip (SCCLL) by immunohistochemitry, and to evaluate the possible role of these subtypes in the development of regional lymph node metastasis and their association with clinical and pathological parameters. Forty-two cases of SCCLL were divided into two groups (21 with and 21 without regional lymph node metastasis). The histopathological grade of malignancy was determined and the material was submitted to double staining with anti-CD68/anti-CD163 and anti-CD68/anti-HLA-DR monoclonal antibodies. The results were analyzed statistically using the Wilcoxon signed-rank and Spearman correlation tests. The M1 and M2 subpopulations were observed in all cases studied. No significant difference was observed between the quantities of M1 and M2 TAMs regarding tumor size (p > 0.05). A significantly larger number of M2 compared to M1 TAMs was observed in tumors without regional lymph node metastasis, tumors in early stages, and low-grade tumors (p < 0.05). No significant difference between the numbers of M1 and M2 TAMs was observed in tumors with regional lymph node metastasis, tumors in advanced stages, and high-grade tumors (p > 0.05). There was a positive weak correlation between M1 and M2 TAMs (r = 0.361; p = 0.019). The results suggest a more important role of M2 TAMs in early stages than advanced stages of lip carcinogenesis. The progression of SCCLL does not seem to be related to an imbalance of macrophage polarization in the microenvironment of these tumors.
Descritores: Neoplasias Labiais/patologia
Carcinoma de Células Escamosas/patologia
Macrófagos/patologia
-Valores de Referência
Imuno-Histoquímica
Antígenos de Diferenciação Mielomonocítica
Antígenos HLA-DR
Antígenos CD
Contagem de Células
Estudos Retrospectivos
Inclusão em Parafina
Receptores de Superfície Celular
Estatísticas não Paramétricas
Gradação de Tumores
Carcinogênese
Metástase Linfática
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


  5 / 620 LILACS  
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Id: biblio-1001602
Autor: França, Glória Maria De; Carmo, Andréia Ferreira do; Costa Neto, Hugo; Andrade, Ana Luiza Dias Leite de; Lima, Kenio Costa de; Galvão, Hébel Cavalcanti.
Título: Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects
Fonte: Braz. oral res. (Online);33:e047, 2019. tab, graf.
Idioma: en.
Resumo: Abstract: The aim of this study was to evaluate macrophage M1 and M2 subpopulations in radicular cysts (RCs) and periapical granulomas (PGs) and relate them to clinical and morphological aspects. M1 macrophages were evaluated by the percentage of CD68 immunostaining associated with the inflammatory cytokine TNF-α, and M2 macrophages, by its specific CD163 antibody. The CD68+/CD163+ ratio was adopted to distinguish between the two macrophage subpopulations. Clinical, radiographic, symptomatology, treatment, and morphological parameters of lesions were collected and a significance level of p = 0.05 was adopted for statistical analysis. The results showed that the CD68+/CD163+ ratio was higher in the RCs (median = 1.22, p = 0.002), and the highest TNF-α immunostaining scores were found in RCs (p = 0.018); in PGs, the CD68+/CD163+ ratio was lower and associated with a greater CD163+ immunostaining (median = 1.02, p <0.001). The TNF-α in cyst epithelium had a score of 3 in 10 cases and predominance of M1 macrophages by CD68+/CD163+ (median = 2.23). In addition, CD68+ cells had higher percentage of immunostaining in smaller RCs (p = 0.034). Our findings suggest that increased CD68 immunostaining associated with TNF-α cytokine in RCs results in a greater differentiation of the M1 phenotype. The higher CD163 immunostaining in PGs results in greater differentiation of the M2 phenotype. Therefore, the inflammatory state promoted by M1 macrophages is related to growth and progression of RCs; on the other hand, the immunomodulatory state of M2 macrophages is related to maintenance of PGs.
Descritores: Granuloma Periapical/patologia
Cisto Radicular/patologia
Macrófagos/patologia
-Valores de Referência
Imuno-Histoquímica
Antígenos de Diferenciação Mielomonocítica/análise
Antígenos CD/análise
Doença Crônica
Fator de Necrose Tumoral alfa/análise
Receptores de Superfície Celular/análise
Estatísticas não Paramétricas
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME


  6 / 620 LILACS  
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Id: lil-478290
Autor: Lima, Rafael Rodrigues; Costa, Ana Maria Rabelo; Souza, Renata Duarte de; Gomes-Leal, Walace.
Título: Inflamação em doenças neurodegenerativas / Inflammation in neurodegenerative disease
Fonte: Rev. para. med = Rev. Para. Med. (Impr.);21(2):29-34, abr.-jun. 2007.
Idioma: pt.
Resumo: Objetivo: estudo descritivo sobre a atuação do processo inflamatório em doenças neurodegenerativas, com ênfase nos dois principais tipos celulares envolvidos: neutrófilo e macrófago. Método: pesquisa do tema nas fontes bibliográficas na base de dados PUBMED/MEDLINE. Considerações Finais: existem diversas evidências na literatura que mostram que, embora os mecanismos inflamatórios participem dos fenômenos de reparação tecidual, também, estão envolvidos em processos de degeneração secundária em doenças agudas e crônicas do sistema nervoso central.

Objective: to describe the performance of the inflammatory process in neurodegenerative disease, with emphasis in the two main involved cellular types: neutrophil and macrophage. Method: through bibliographical search in the databases PUBMED/MEDLINE. Final Considerations: diverse evidences in literature exist showing that even so the inflammatory mechanisms participate of the phenomena of tecidual repairing, also are involved in processes of secondary degeneration in acute and chronic disease of the central nervous system.
Descritores: Doenças Neurodegenerativas
Inflamação
Macrófagos
Neutrófilos
Tipo de Publ: Revisão
Responsável: BR3.1 - Biblioteca Central


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Id: biblio-838972
Autor: Espinoza, Rodrigo A; Silva-Valenzuela, Cecilia A; Amaya, Fernando A; Urrutia, Ítalo M; Contreras, Inés; Santiviago, Carlos A.
Título: Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages
Fonte: Biol. Res;50:5, 2017. tab, graf.
Idioma: en.
Projeto: FONDECYT; . FONDECYT; . CONICYT; . CONICYT; . CONICYT.
Resumo: BACKGROUND: Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages. RESULTS: Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi with human or murine macrophages. Of note, the presence of an intact copy of SPI-13 in a S. Typhi mutant carrying a deletion of SPI-8 did not improve its ability to be internalized by, or survive in human or murine macrophages. CONCLUSIONS: Altogether, our results point out to different roles for SPI-13 and SPI-8 during Salmonella infection. While SPI-13 contributes to the interaction of S. Enteritidis with murine macrophages, SPI-8 is not required in the interaction of S. Typhi with murine or human macrophages. We hypothesized that typhoid serovars have lost SPI-13 and maintained SPI-8 to improve their fitness during another phase of human infection.
Descritores: Salmonella enteritidis/genética
Infecções por Salmonella/microbiologia
Salmonella typhi/genética
Ilhas Genômicas/fisiologia
Macrófagos/microbiologia
-Especificidade da Espécie
Sobrevivência Celular
Células Cultivadas
Reação em Cadeia da Polimerase
Análise de Variância
Genoma Bacteriano
Fenômenos Fisiológicos Bacterianos
Ilhas Genômicas/genética
Interações Microbianas/genética
Sorogrupo
Células RAW 264.7
Muridae
Limites: Humanos
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


  8 / 620 LILACS  
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Id: biblio-950728
Autor: Castro, Jenny P; Ocampo, Yanet C; Franco, Luis A.
Título: In vivo and in vitro anti-inflammatory activity of Cryptostegia grandiflora Roxb. ex R. Br. leaves
Fonte: Biol. Res;47:1-8, 2014. ilus, graf, tab.
Idioma: en.
Projeto: University of Cartagena.
Resumo: BACKGROUND: Despite Cryptostegia grandiflora Roxb. ex R. Br. (Apocynaceae) leaves are widely used in folk Caribbean Colombian medicine for their anti-inflammatory effects, there are no studies that support this traditional use. Therefore, this work aimed to evaluate the effect of the total extract and primary fractions obtained from Cryptostegia grandiflora leaves, using in vivo and in vitromodels of inflammation, and further get new insights on the mechanisms involved in this activity. RESULTS: Ethanolic extract of Cryptostegia grandiflora leaves, and its corresponding ether and dichloromethane fractions, significantly reduced inflammation and myeloperoxidase activity (MPO) in ear tissue of mice treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Histological analysis revealed a reduction of edema and leukocyte infiltration. Complementarily, we demonstrated that extract and fractions reduced nitric oxide (NO•) and prostaglandin E2 (PGE2) production in LPS-stimulated RAW 264.7 macrophages, as well as scavenging activity on DPPH and ABTS radicals. CONCLUSIONS: Our results demonstrated for the first time the anti-inflammatory activity of Cryptostegia grandiflora leaves, supporting its traditional use. This activity was related to inhibition of MPO activity, and PGE2 and NO• production. These mechanisms and its antioxidant activity could contribute, at least in part, to the anti-inflammatory effect showed by this plant.
Descritores: Extratos Vegetais/uso terapêutico
Apocynaceae/química
Edema/tratamento farmacológico
Macrófagos/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
-Ocitócicos/análise
Dinoprostona/análise
Peroxidase/antagonistas & inibidores
Folhas de Planta/química
Citotoxinas/farmacologia
Linhagem Celular Tumoral/efeitos dos fármacos
Inflamação/tratamento farmacológico
Camundongos Endogâmicos ICR
Óxido Nítrico/análise
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 620 LILACS  
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Id: biblio-950738
Autor: Kim, Sung-Jo; Hong, Minho; Song, Ki Duk; Lee, Hak-Kyo; Ryoo, Sungweon; Heo, Tae-Hwe.
Título: Normalization of the levels of inflammatory molecules in Mycobacterium smegmatis-infected U937 cells by fibrate pretreatment
Fonte: Biol. Res;47:1-9, 2014. ilus, graf.
Idioma: en.
Projeto: The Catholic University of Korea. Research Fund 2011; . Republic of Korea. Development Administration. BioGreen 21.
Resumo: BACKGROUND: Tuberculosis (TB) is a respiratory tract disease caused by Mycobacterium tuberculosis infection. M. tuberculosis exploits immune privilege to grow and divide in pleural macrophages. Fibrates are associated with the immune response and control lipid metabolism through glycolysis with ß-oxidation of fatty acids. RESULTS: In this study, we investigated the effect of fibrate pretreatment on the immune response during M. smegmatis infection in U937 cells, a human leukemic monocyte lymphoma cell line. The protein expression of tumor necrosis factor α (TNF-α), an inflammatory marker, and myeloid differentiation primary response gene 88 (MyD88), a toll like receptor adaptor molecule, in the infected group increased at 1 and 6 h after M. smegmatis infection of U937 cells. Acetyl coenzyme A acetyl transferase-1 (ACAT-1), peroxisome proliferator-activated receptor-α (PPAR-α), TNF-α, and MyD88 decreased in U937 cells treated with fibrates at 12 and 24 h after treatment. More than a 24 h pretreatment with fibrate resulted in similar expression levels of ACAT-1 and PPAR-α between infected vehicle control and infected groups which were pretreated with fibrate for 24 h. However, upon exposure to M. smegmatis, the cellular expression of the TNF-α and MyD88 in the infected groups pretreated with fibrate for 24 h decreased significantly compared to that in the infected vehicle group. CONCLUSION: These results suggest that fibrate pretreatment normalized the levels of inflammatory molecules in Mycobacterium smegmatis-infected U937 cells. Further studies are needed to confirm the findings on pathophysiology and immune defense mechanism of U937 by fibrates during M. tuberculosis infection.
Descritores: Mediadores da Inflamação/metabolismo
Mycobacterium smegmatis
Ácidos Fíbricos/farmacologia
Macrófagos/efeitos dos fármacos
Infecções por Mycobacterium/metabolismo
-Acetil-CoA C-Acetiltransferase/metabolismo
Transdução de Sinais/efeitos dos fármacos
Western Blotting
Fator de Necrose Tumoral alfa/metabolismo
Células U937
PPAR alfa/metabolismo
Fator 88 de Diferenciação Mieloide/metabolismo
Macrófagos/metabolismo
Macrófagos/microbiologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  10 / 620 LILACS  
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Id: biblio-950765
Autor: Park, Hee-Sook; Choi, Hye-Young; Kim, Gun-Hee.
Título: Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264. 7 macrophages depending on cooking method
Fonte: Biol. Res;47:1-6, 2014. graf, tab.
Idioma: en.
Projeto: Globalization of Korean Foods R& D program; . Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea; . National Research Foundation of Korea; . Ministry of Education. Basic Science Research Program.
Resumo: BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 µg/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on NO production, pan-fried LF showed significant attenuation of the expression of inducible nitiric oxide synthase (iNOS) compared with fresh LF. In the cooking method of LF, PGE2 production was not affected in the LPS-induced RAW 264.7 cells. In LPS-induced RAW 264.7 cells, pretreatment by fresh and cooked LF increased COX2 mRNA expression. The 3-O-caffeoylquinic acid content of blanching and pan-frying LF increased by 4.92 and 9.7 fold with blanching and pan-frying respectively in comparison with uncooked LF. CONCLUSIONS: Regardless of the cooking method, Ligularia fischeri exhibited potent inhibition of NO production through expression of iNOS in LPS-induced RAW264.7 cells.
Descritores: Culinária/métodos
Asteraceae/química
Preparações de Plantas/farmacologia
Óxido Nítrico Sintase Tipo II/metabolismo
Macrófagos/efeitos dos fármacos
Óxido Nítrico/biossíntese
-Ácido Quínico/análise
Ácido Quínico/análogos & derivados
Ácido Quínico/classificação
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Dinoprostona/análise
Dinoprostona/biossíntese
Sobrevivência Celular
Lipopolissacarídeos
Cromatografia Líquida de Alta Pressão
Asteraceae/classificação
Ciclo-Oxigenase 2/análise
Ciclo-Oxigenase 2/metabolismo
Células RAW 264.7
Temperatura Alta
Macrófagos/fisiologia
Anti-Inflamatórios/farmacologia
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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