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Id: biblio-1170624
Autor: Lozano de Luaces, Vicente; Murtra Ferré, Jaime; Espías Gómez, Angel.
Título: Hiperemia pulpar o pulpitis transicional
Fonte: Rev. Círc. Odontol. Córdoba;44(1):27-32, jul. 1986. ilus.
Idioma: es.
Descritores: Odontoblastos
Pulpite
Limites: Humanos
Responsável: AR29.1 - Biblioteca


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Id: biblio-1052229
Autor: Simancas-Escorcia, Víctor Hugo.
Título: Fisiopatología de los odontoblastos: una revisión / Odontoblast pathophysiology: a review
Fonte: Duazary;16(3):87-103, 2019. tab, ilus.
Idioma: es.
Resumo: Los odontoblastos son células posmitóticas de origen mesenquimal dispuestas en forma de palizada en la periferia de la pulpa dental y responsables de la formación de la dentina. Los odontoblastos derivan de la cresta neural,y su diferenciación es la consecuencia de las interacciones epitelio-mesénquima entre las células de la papila dental y el epitelio dental interno. Este trabajo tiene como objetivo revisar los aspectos fisiológicos y patológicos de los odontoblastos, comprendiendo su origen, mecanismos de diferenciación y propiedades funcionales. Se realizó una búsqueda electrónica de literatura desde el año 2000 hasta febrero de 2018y seseleccionaron 2.889 artículos, de los cuales 52 fueron analizados y discutidos. Los resultados exponen el origen, las etapas y los factores relacionados con la diferenciación odontoblástica, junto con los aspectos principales de la organización estructural y las funciones que desempeñan los odontoblastos. Esta revisión demuestra mediante la evidencia científica actual cómo los estudios concernientes a los odontoblastos se focalizan en comprender los mecanismos en la formación de la dentina reparativa, la respuesta inmunitaria y su rol en los procesos de inflamación y dolor. Trabajos futuros deberán esclarecer las diferentes señales involucradas en los procesos fisiopatológicos celulares y moleculares llevados a cabo por los odontoblastos.

The odontoblasts are post-mitotic cells of mesenchymal origin arranged in the form of a palisade in the periphery of the dental pulp and responsible for the formation of the dentin. The odontoblasts are derived from the neural crest and their differentiation is the consequence of epithelial-mesenchymal interactions between the cells of the dental papilla and the internal dental epithelium. This work aims to review the physiological and pathological aspects of odontoblasts, including their origin, mechanismsof differentiation and functional properties. An electronic literature search was conducted from 2000 to February 2018, selecting 2889articles, of which 52 articles were analyzed and discussed. The results show the origin, stages and factors related to odontoblastic differentiation, together with the main aspects of the structural organization and functions performed by odontoblasts. This review demonstrates through current scientific evidence that the studies concerning odontoblasts focus on understanding the mechanisms in the formation of reparative dentin, the immune response and its role in the processes of inflammation and pain. Future work should clarify the different signals involved in the cellular and molecular pathophysiological processes carriedout by the odontoblasts.
Descritores: Odontoblastos
Tipo de Publ: Revisão
Responsável: CO644.1 - Biblioteca Germán Bula Meyer


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Texto completo SciELO Brasil
Briso, André Luiz Fraga
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Id: lil-779901
Autor: CINTRA, Luciano Tavares Angelo; BENETTI, Francine; FERREIRA, Luciana Louzada; RAHAL, Vanessa; ERVOLINO, Edilson; JACINTO, Rogério de Castilho; GOMES FILHO, João Eduardo; BRISO, André Luiz Fraga.
Título: Evaluation of an experimental rat model for comparative studies of bleaching agents
Fonte: J. appl. oral sci;24(2):171-180, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP; . FAPESP.
Resumo: ABSTRACT Dental materials in general are tested in different animal models prior to the clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents, by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats’ vital teeth. Material and Methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals were untreated (control). The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated through histomorphometric analysis with inflammatory cell count in the coronal and radicular thirds of the pulp. Fibroblasts were also counted. Scores were attributed to odontoblastic layer and vascular changes. Tertiary dentin area and pulp chamber central area were measured histomorphometrically. Data were compared by analysis of variance and Kruskal-Wallis test (p<0.05). Results After 2 days, the amount of inflammatory cells increased in the coronal pulp occlusal third up to the 15-min application groups of each bleaching gel. In the groups exposed to each concentration for 30 and 45 min, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, reduction on the pulp chamber central area and enlargement of the tertiary dentin area were observed, without the detection of inflammation areas. Conclusion The rat model of extracoronal bleaching showed to be adequate for studies of bleaching protocols, as it was possible to observe alterations in the pulp tissues and tooth structure caused by different concentrations and application periods of bleaching agents.
Descritores: Clareamento Dental/métodos
Polpa Dentária/efeitos dos fármacos
Clareadores Dentários/administração & dosagem
Peróxido de Hidrogênio/administração & dosagem
-Fatores de Tempo
Contagem de Células
Reprodutibilidade dos Testes
Ratos Wistar
Modelos Animais
Polpa Dentária/patologia
Cavidade Pulpar
Fibroblastos/efeitos dos fármacos
Géis
Odontoblastos/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Publicação Retratada
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Briso, André Luiz Fraga
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Id: lil-777358
Autor: Cintra, Luciano Tavares Angelo; Benetti, Francine; Ferreira, Luciana Lousada; Rahal, Vanessa; Ervolino, Edilson; Jacinto, Rogério de Castilho; Gomes Filho, João Eduardo; Briso, André Luiz Fraga.
Título: Evaluation of an experimental rat model for comparative studies of bleaching agents
Fonte: J. appl. oral sci;24(1):95-104, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP’s; . FAPESP’s.
Resumo: ABSTRACT Dental materials, in general, are tested in different animal models prior to their clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats’ vital teeth. Material and methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals (control) were untreated. The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated by histomorphometric analysis with inflammatory cell counting in the coronal and radicular thirds of the pulp. The counting of fibroblasts was also performed. Scores were attributed to the odontoblastic layer and to vascular changes. The tertiary dentin area and the pulp chamber central area were histomorphometrically measured. Data were compared by the analysis of variance and the Kruskal-Wallis test (p<0.05). Results After 2 days, the amount of inflammatory cells increased in the occlusal third of the coronal pulp until the time of 15 min for both concentrations of bleaching gels. In 30 and 45 min groups of each concentration, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, a reduction in the pulp chamber central area and an enlargement of tertiary dentin area were observed without the detection of inflammation areas. Conclusion The rat model of extra coronal bleaching showed to be adequate for bleaching protocols studies, as it was possible to observe alterations in the pulp tissues and in the tooth structure caused by different concentrations and periods of application of bleaching agents.
Descritores: Clareamento Dental/métodos
Polpa Dentária/efeitos dos fármacos
Clareadores Dentários/administração & dosagem
Peróxido de Hidrogênio/administração & dosagem
-Fatores de Tempo
Contagem de Células
Reprodutibilidade dos Testes
Ratos Wistar
Modelos Animais
Polpa Dentária/patologia
Cavidade Pulpar
Fibroblastos/efeitos dos fármacos
Géis
Odontoblastos/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Costa, Carlos Alberto de Souza
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Id: biblio-893660
Autor: Duque, Cristiane; Aida, Kelly Limi; Pereira, Jesse Augusto; Teixeira, Gláucia Schuindt; Caldo-Teixeira, Angela Scarparo; Perrone, Luciana Rodrigues; Caiaffa, Karina Sampaio; Negrini, Thais de Cássia; Castilho, Aline Rogéria Freire de; Costa, Carlos Alberto de Souza.
Título: In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment
Fonte: J. appl. oral sci;25(5):541-550, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Projeto: Rio de Janeiro State Research Foundation (FAPERJ).
Resumo: Abstract Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed using agar diffusion and anti-biofilm assays. Cytotoxic effects, compressive tensile strength, microhardness and fluoride (F) release were also evaluated. A randomized controlled trial was conducted on 36 children that received ART either with GIC or GIC with CHX. Saliva and biofilm were collected for mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% and 2.5% CHX improved the antimicrobial/anti-biofilm activity of GIC, without affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A significant reduction of MS levels was observed for KM+CHX group in children saliva and biofilm 7 days after treatment. Conclusions: The incorporation of 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations.
Descritores: Clorexidina/farmacologia
Clorexidina/química
Tratamento Dentário Restaurador sem Trauma/métodos
Cimentos de Ionômeros de Vidro/farmacologia
Cimentos de Ionômeros de Vidro/química
Anti-Infecciosos Locais/farmacologia
-Valores de Referência
Saliva/microbiologia
Streptococcus mutans/crescimento & desenvolvimento
Streptococcus mutans/efeitos dos fármacos
Resistência à Tração
Fatores de Tempo
Técnicas In Vitro
Teste de Materiais
Candida albicans/crescimento & desenvolvimento
Candida albicans/efeitos dos fármacos
Contagem de Colônia Microbiana
Reprodutibilidade dos Testes
Análise de Variância
Resultado do Tratamento
Estatísticas não Paramétricas
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Força Compressiva
Fluoretos/química
Testes de Dureza
Lactobacillus acidophilus/crescimento & desenvolvimento
Lactobacillus acidophilus/efeitos dos fármacos
Odontoblastos/efeitos dos fármacos
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: biblio-893619
Autor: TANG, Jia; SAITO, Takashi.
Título: Human plasma fibronectin promotes proliferation and differentiation of odontoblast
Fonte: J. appl. oral sci;25(3):299-309, May-June 2017. graf.
Idioma: en.
Projeto: Japanese Society for the Promotion of Science; . Japanese Society for the Promotion of Science.
Resumo: Abstract Objective To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro. Material and Methods Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkaline phosphatase activity (ALP activity), mRNA expression of hard tissue-forming markers, and Alizarin red staining were investigated over a period of 10 days. Results Cells maintained a high proliferation activity on Fn and PCOL even at a low seeding concentration (0.5×104/mL) as demonstrated by CCK-8 assay. The proliferation activity of cells on Fn increases in a concentration-dependent manner while it reached a plateau after 10 µg/mL. Cells adopted long, thin and spindle shape on Fn(10-50) and PCOL. Parallel actin filaments were observed in MDPC-23 cells cultured on Fn and PCOL. ALP activity was markedly up-regulated on Fn and PCOL-coated surfaces. Importantly, gene expression of BSP (Fn10: 2.44±0.32; Fn20: 3.05±0.01; Fn30: 2.90±0.21; Fn40: 2.74±0.30; Fn50: 2.64±0.12; PCOL: 2.20±0.03) and OCN (Fn10: 2.52±0.23; Fn20: 2.28±0.24; Fn30: 2.34±0.21; Fn40: 2.34±0.25; Fn50: 2.20±0.22; PCOL: 1.56±0.16) was significantly enhanced on Fn and PCOL substrates as compared with control; moreover, expression of integrin beta 1 (ITGB1), an ubiquitous cell surface receptor was augmented in Fn(10-50) and PCOL groups simultaneously. In accordance with the ALP activity and gene expression data, calcific deposition in cells grown on Fn(10-50) and PCOL was observed as well. Conclusion Despite the limitation of this study, the findings indicate that a surface coating of Fn enhances the proliferation, differentiation and mineralization of odontoblast-like cells by activation of integrin beta 1 (ITG B1). The promoting effects of Fn on MDPC-23 cells were achieved at a comparatively lower coating concentration than type I collagen (300 µg/mL). Specifically, it is suggested that the optimum coating concentration of Fn to be 10 µg/mL.
Descritores: Diferenciação Celular/efeitos dos fármacos
Fibronectinas/farmacologia
Proliferação de Células/efeitos dos fármacos
Odontoblastos/efeitos dos fármacos
-Fatores de Tempo
Expressão Gênica
Células Cultivadas
Reprodutibilidade dos Testes
Imunofluorescência
Antraquinonas
Integrina beta1/farmacologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Colágeno Tipo I/farmacologia
Fosfatase Alcalina/análise
Limites: Humanos
Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-998237
Autor: Machmud, Edy; Jubhari, Eri Hendra; Ardiansyah, Muchammad.
Título: Histometry Analysis of Osteoclasts, Osteoblasts and Fibroblasts in Immediate Placement Implants with Addition of Aloe Vera Extract
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4537, 01 Fevereiro 2019. graf, tab.
Idioma: en.
Resumo: Objective: To analyze the effect of immediate placement of implants with extract from the new bone formation histometically. Material and Methods: In this true-experimental design with randomized post test control group, 9 mongrel dogs weighing 10 to 12 kg were used, which were divided into 3 groups, based on observation time of 14 days, 28 days and 56 days. On the installation of implants (∅3.5x10 mm) sequentially, the former socket extraction of the lower jaw's right second premolar tooth in the study sample injected 10% Aloe vera gel extract and left second left premolar tooth without injection of 10% Aloe vera extract. To compare independent groups use the Mann-Whitney test. All analysis were carried out using SPSS version 20. Results: There was an increase in the number of osteoblast cells in both treatment and control groups, but the value of the treatment group was greater. There were significant differences in the number of osteoblast cells between the treatment and control groups 14 days (p=0.019), 28 days: (p=0.018), and 56 days (p=0.009). There were no significant differences in the number of fibroblast cells between the treatment and control groups (p>0.05). But at observations 28 and 56 days, it was showed a significant difference in the number of fibroblast cells between the treatment and control groups (p=0.353 and p=0.024, respectively). Conclusion: Immediate placement of implants with 10% Aloe vera extract gel on extracted socket increases the number of osteoblasts and suppresses the number of osteoclasts and fibroblasts.
Descritores: Osteoclastos
Células do Tecido Conjuntivo
Implantação Dentária Endo-Óssea
Aloe
-Estatísticas não Paramétricas
Fibroblastos
Indonésia
Odontoblastos
Limites: Animais
Cães
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-1000087
Autor: Alano Díaz, S; Villegas Padilla, K. M; Mandalunis, Patricia Mónica.
Título: Alteraciones de la dentina con el envejecimiento / Dentin alterations with aging
Fonte: Rev. Fac. Odontol. (B.Aires);33(75):29-35, jul.-dic. 2018. ilus.
Idioma: es.
Resumo: Diferentes estudios han demostrado que después de la tercera década de vida hay una transición en la microestructura de la dentina. Dada la importancia de ésta como sustrato para la adhesión de materiales de restauración utilizados en operatoria y rehabilitación oral, ha sido objetivo de este trabajo realizar una búsqueda bibliográfica de las publicaciones existentes en inglés y español de los últimos 15 años, haciendo uso de buscadores científicos como Pubmed, Google Schoolar y LILACS que permitieran actualizar la información existente ayudando a entender los efectos biológicos del envejecimiento de la dentina, identificando los cambios de mayor relevancia a nivel histológico. De la búsqueda realizada se concluye que el envejecimiento de la dentina está asociado con aumento de dentina secundaria, formación de dentina esclerótica opaca, variaciones en la composición química de la matriz y disminución del número y actividad de los odontoblastos. Los conocimientos sobre el envejecimiento de la dentina deben tenerse en cuenta frente a estudios relacionados con materiales de restauración dental, ya que los cambios en la microestructura y capacidad funcional de la dentina con el envejecimiento requieren que éstos se adapten a dichas variaciones (AU)

Different studies have shown that aafter the third term of life there is a transition in the microstructure of dentin. Given the importance of dentin as a substrate for the adhesion of restorative materials used in operative and oral rehabilitation, the aim of the present work was to conduct a search of the scientific literature in English and Spanish, published in the last 15 years, using search engines such as Pubmed, Google Schoolar and LILACS, for an update on the biological effects of dentin aging, to identify the most relevant age-related histological changes in dentina. The data obtained from the literature review allow concluding that dentin aging is associated with an increase in secondary dentina, opaque sclerotic dentin formation, variations in the chemical composition of the matrix and a decrease in odontoblast number and activity. Updated information on dentin aging should be taken into account in studies on dental restoration materials, since the latter must adapt to aging-related changes in the microstructure and functional capacity of dentin (AU)
Descritores: Envelhecimento/fisiologia
Dentina/fisiopatologia
Odontoblastos
-Adesivos Dentinários
Materiais Dentários
Restauração Dentária Permanente
Dentinogênese
Limites: Humanos
Tipo de Publ: Revisão
Responsável: AR29.1 - Biblioteca


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Texto completo SciELO Chile
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Id: lil-708740
Autor: Morales, Rolando; Trujillo, Erick; Cantín, Mario.
Título: Caracterización estereológica de odontoblastos en pulpas dentarias humanas sanas y con pulpitis reversible / Stereological characterization of odontoblasts in normal healthy and reversible pulpitis in human dental pulps
Fonte: Int. j. morphol;32(1):154-160, Mar. 2014. ilus.
Idioma: es.
Resumo: La reacción y reparación dentinaria depende del número de odontoblastos. Los métodos para obtener estimaciones fiables sobre la cantidad de odontoblastos en la pulpa dental han sido subjetivos y sesgados, sobre todo al evaluar los cambios cuantitativos y la potencial capacidad reparativa en presencia de caries. El objetivo de este trabajo fue estimar y comparar cuantitativamente el número, densidad y volumen de odontoblastos en dientes sanos y con diagnóstico de pulpitis reversible producto de caries a través de herramientas estereológicas. Se utilizaron dientes premolares humanos obtenidos de exodoncias, divididos en un grupo sano y otro cariado. Fueron fijados y descalcificados con ácido nítrico al 5%. Siguiendo el protocolo del orientator se obtuvieron 5 secciones de 5 mm teñidas por H-E de cada diente. Se aplicó el recuento estereológico de los odontoblastos con el test multipropósito M42. Se estimaron las densidades de número (Nv), volumen (Vv) y superficie (Sv), y calcularon las Medias (±DE) por diente, y Medias (±EE) por grupo. Las diferencias entre grupos se analizaron mediante la prueba T, con un valor p 0,05 de significación estadística. En dientes sanos, la Media (±EE) para Nv de odontoblastos fue 0,409x105/mm3 (±0,018x105/mm3), para Vv 19,714% (±1,43%) y para Sv 21,016 mm2/mm3 (±1,425 mm2/mm3). En dientes cariados, la Nv fue 0,521x105 /mm3 (±0,023x105/mm3), la Vv 24,686% (±1,625%) y la Sv 23,203 mm2/mm3 (±1,364 mm2/mm3). Al comparar las Nv, los odontoblastos del grupo con caries aumentaron significativamente (p=0,0062), al igual que la Vv (p=0,0197). Existe un aumento del número de odontoblastos en los dientes con pulpitis reversible, lo que condicionaría su capacidad de respuesta. La metodología empleada puede ser aplicable para determinar el comportamiento pulpar y cuantificar variables de respuesta odontoblástica en tratamientos restauradores atraumáticos de manera imparcial y reproducible.

Dentinal reaction and repair depends on factors like the amount of odontoblasts. Methods for obtaining reliable estimates of the number of odontoblasts in the dental pulp have been subjective and biased, especially when assessing the potential quantitative changes and reparative capacity in the presence of cavities. The aim of this study was to estimate and quantitatively compare, through stereological tools, the number, density and volume of odontoblasts in healthy teeth, diagnosed with reversible pulpitis due to caries. We used human premolars obtained from extractions, divided into groups of healthy teeth and teeth with caries, fixed and decalcified in 5% nitric acid. Following the orientator protocol, five 5-µm-thick sections stained by HE were obtained from each tooth. Stereological counting for odontoblast with M42 multipurpose test was applied. Numerical density (Nv), volume density (Vv) and surface density (Sv) were estimated, and the mean (± SD) for each tooth, and Mean (± SE) per group were calculated. Differences between groups were analyzed by t test, with p 0.05 for statistical significance. In the healthy teeth group, the mean (± SE) for Nv was 0.409x105/mm3 (±0.018x105/mm3), Vv 19.714% (±1.43%) and to Sv 21.016 mm2/mm3 (±1.425 mm2/mm3) odontoblast cells. In the caries teeth group, the Nv was 0.521x105 /mm3 (±0.023x105/mm3), Vv 24.686% (±1.625%) and Sv 23.203 mm2/mm3 (±1.364 mm2/mm3). When comparing Nv, an increased in odontoblasts significantly (p = 0.0062), as well as Vv (p = 0.0197) in caries teeth group. There is an increased number of odontoblasts in teeth with reversible pulpitis, which would condition its responsiveness. The methodology can be applied to determine pulp behavior, and quantify variables of odontoblastic response in atraumatic restorative treatments in an impartial and reproducible form.
Descritores: Polpa Dentária/anatomia & histologia
Polpa Dentária/citologia
Odontoblastos
-Pulpite
Estudos Transversais
Limites: Humanos
Masculino
Adolescente
Responsável: CL1.1 - Biblioteca Central


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Id: lil-734668
Autor: Gutiérrez Cantú, Francisco Javier; Mariel Cárdenas, Jairo; Oliva Rodríguez, Ricardo; Romo Ramírez, Gabriel Fernando; Sánchez Méraz, Wulfrano; Mariel Murga, Humberto; Leal Tobias, Luis Armando.
Título: Expression of Mmp-20 in dental germs of human fetus / Expresion de Mmp-20 en gérmenes dentales de fetos humanos
Fonte: Int. j. morphol;32(4):1261-1265, Dec. 2014. ilus.
Idioma: en.
Resumo: During experiments in animal studies, it has been observed that enamelysin (MMP-20) is expressed during tooth development in the late secretory stage of amelogenesis but not in the mature enamel.The aim of this research was to determine the location of MMP-20 in human tooth germs in the different structures of the enamel organ.The detection of MMP-20 was performed by immunohistochemistry in 20 specimens obtained from human fetuses. Immunostaining of MMP-20 was observed from the presecretor stadium in stellate reticulum and intermediate stratum and in the basal portion of ameloblasts in the secretory stage in stellate reticulum, stratum intermedium, secretory ameloblasts, odontoblasts and dental papilla. The results of this research show the location of MMP-20 in tooth germ development in humans and provides the foundation for future research about the process of dental organ formation.

En estudios realizados en animales de experimentación se ha observado que la enamelisina (MMP-20) se expresa durante el desarrollo dental durante el estadio de secreción tardío de la amelogénesis pero no en el esmalte maduro. El objetivo de la presente investigación fue determinar la localización de MMP-20 en gérmenes dentarios humanos en las diferentes estructuras del órgano del esmalte. Se analizaron 20 especímenes obtenidos de fetos humanos, efectuando la detección de MMP-20 por Inmunohistoquímica. Se observó inmunolocalización de MMP-20 desde el estadio presecretor en retículo estrellado y estrato intermedio, así como en porción basal de ameloblastos; en el estadio secretor en retículo estrellado, estrato intermedio, ameloblastos secretores, odontoblastos y papila dental. Los resultados de la presente investigación muestran la localización de la MMP-20 en el desarrollo del germen dentario en humanos y aporta las bases para futuras investigaciones acerca del proceso de formación de los órganos dentales.
Descritores: Germe de Dente/enzimologia
Metaloproteinase 20 da Matriz/metabolismo
-Germe de Dente/embriologia
Imuno-Histoquímica
Feto
Ameloblastos
Odontoblastos
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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