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Id: biblio-1056419
Autor: Rivera, Christopher; Tuemmers, Christian; Bañados, Rodrigo; Vidal-Seguel, Nicolás; Montiel-Eulefi, Enrique.
Título: Reduction of recurrent tendinitis scar using autologous mesenchymal stem cells derived from adipose tissue from the base of the tail in Holsteiner horses (Equus ferus caballus) / Reducción de la cicatriz de tendinitis recidivante mediante células madre mesenquimales autólogas derivadas de tejido adiposo de la base de la cola en equinos Holsteiner (Equus ferus caballus)
Fonte: Int. j. morphol;38(1):186-192, Feb. 2020. graf.
Idioma: en.
Projeto: Universidad Católica de Temuco.
Resumo: As a result of their intense physical activity, racehorses suffer high tendon stress which may result in various pathologies. One of these is tendonitis in the tendon of the superficial digital flexor muscle (TSDFM). Conventional treatment with rest, has not shown to be very effective, and regenerative medicine through the application mesenchymal stem cells appears to be a promising therapy. The objective of this work was to assess the effect of the application of autologous MSC on reduction of the scar length in recurrent TSDFM tendinitis in Holsteiner horses, using image analysis. This study included two groups of five animals each: A control group that received conventional treatment (CG) and an experimental group which was also treated with intralesional injections of MSC (EG). Scar evolution was assessed by echographic analysis, with measurements taken of the scar length over a four month period; the length at month zero, was taken as the initial value of 100 %. During the first month, the mean scar length diminished to 81.14 % (EG) and 95.85 % (CG); after the second month, lengths were 64.4 % (EG) and 92.3 % (CG); following the third month lengths were 51.92 % (EG) and 87.42 % (CG); finally at the end of the fourth month the lengths recorded were 26.7 % (EG) and 83.92 % (CG). These results show that treatment with autologous MSC helps TSDFM scar length was significantly reduced, as compared to conventional treatment.

Reducción de la cicatriz de tendinitis recidivante mediante células Madre mesenquimales autólogas derivadas de tejido adiposo de la base de la cola en equinos Holsteiner (Equus ferus caballus). En equinos deportistas, la actividad física intensa ocasiona gran estrés en los tendones, pudiendo ocasionar diversas patologías como la tendinitis del tendón del músculo flexor digital superficial (TMFDS). El tratamiento convencional con reposo es poco eficaz, siendo la medicina regenerativa a través de la aplicación de células madres mesenquimáticas (MSC) una promisoria terapia. El objetivo de este trabajo, fue evaluar el efecto de la aplicación de MSC autólogas, sobre la reducción de la longitud de la cicatriz en tendinopatías recidivantes del TMFDS en equinos Holsteiner, a través del análisis de imagen. Este estudio conto con dos grupos de cinco animales cada uno, el grupo control mantuvo el tratamiento convencional (GC) y el grupo experimental fue tratado adicionalmente con inyección interlesional de MSC (GE). El análisis ecográfico permitió evaluar la evolución de la cicatriz, a través de la medición de su longitud durante los cuatros meses, tomando la longitud del mes cero como la medición inicial del 100 %. Durante el primer mes, la longitud de la cicatriz se redujo a un 81,14 % (GE) y 95,85 % (GC), al segundo mes la longitud fue de un 64,4 % (GE) y de 92,3 % (GC), al tercer mes, la longitud fue de 51,92 (GE) y un 87,42 (GC), finalmente al cuarto mes la longitud fue de 26,7 % (GE) y del 83,92 % (GC). Estos resultados muestran que el tratamiento con MSC autólogas favorece a la disminución de la longitud de la cicatriz del TMFDS de forma significativa respecto al tratamiento convencional.
Descritores: Cicatrização
Tecido Adiposo
Tendinopatia/terapia
Células-Tronco Mesenquimais
-Recidiva
Modelos Animais de Doenças
Tendinopatia/complicações
Cavalos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-954604
Autor: Tavares, Mónica Maria Ramos; Barbosa, Laura Elisabete Ribeiro.
Título: Adipose tissue-derived stem cells: a new approach to the treatment of Crohn's disease-associated perianal fistulae / Células estaminais derivadas do tecido adiposo: uma nova abordagem no tratamento de fístulas perianais associadas à doença de Crohn
Fonte: J. coloproctol. (Rio J., Impr.);38(3):240-245, July-Sept. 2018.
Idioma: en.
Resumo: ABSTRACT Crohn's disease has an ever-increasing prevalence and incidence, with about 20% of patients developing perianal fistula with significant impact on their quality of life. Despite the medical and surgical treatments currently used, Crohn's-related fistula treatment continues to pose a challenge due to the low rates of efficacy associated with high recurrence rates. Recent clinical trials have shown promising results regarding safety and efficacy of local treatment of this condition with the use of adipose tissue-derived mesenchymal stem cells. Besides being pluripotent and poorly immunogenic, they have immunomodulatory and anti-inflammatory properties, which combined, may accelerate healing. Our main objective is to summarize the clinical trials we found, highlighting the efficacy rates of this therapy and the main limitations we found in the analysis of the results. We conclude that, in perianal fistulas refractory to conventional therapies, the treatment with adipose tissue-derived mesenchymal cells is safe with promising results that may change the current paradigm of Crohn's related fistula treatment.

RESUMO A incidência e a prevalência da doença de Crohn têm aumentado e, ao longo do decurso da doença, cerca de 20% dos doentes irão desenvolver fístulas perianais com impacto significativo na sua qualidade de vida. Apesar dos tratamentos médicos e cirúrgicos utilizados atualmente, o tratamento destas fístulas continua a constituir um desafio com baixas taxas de eficácia e com elevadas taxas de recorrência. Ensaios clínicos recentes têm demonstrado resultados promissores em termos de segurança e eficácia de tratamentos locais destas fístulas com o recurso a células estaminais mesenquimatosas derivadas do tecido adiposo que, além de pluripotentes e pouco imunogênicas, têm capacidades imunomoduladoras e anti-inflamatórias capazes de promover o processo de cicatrização. O objetivo desta revisão sistemática é sumarizar os ensaios clínicos encontrados, realçando as taxas de eficácia desta terapêutica e as principais limitações na análise dos resultados. Concluímos que, nas fístulas perianais refratárias ás terapias convencionais, o tratamento com com celulas estaminais mesenquimatosas derivadas do tecido adiposo é seguro e com resultados promissores que podem mudar o paradigma atual do tratamento das fistulas complexas associadas à Doença de Crohn.
Descritores: Doença de Crohn/patologia
Fístula Retal/terapia
Células-Tronco Mesenquimais/citologia
-Tecido Adiposo/citologia
Resultado do Tratamento
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão Sistemática
Responsável: BR545.3 - Biblioteca ICBS


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Id: biblio-839349
Autor: Martins, Gabrielle R; Marinho, Rebeca C; Bezerra Junior, Rosivaldo Q; Alves, Antoniel de O; Câmara, Lilia M. C; Albuquerque-Pinto, Luiz C; Teixeira, Maria F. da S.
Título: Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro
Fonte: Braz. j. microbiol;48(1):125-131, Jan.-Mar. 2017. graf.
Idioma: en.
Projeto: National Council of Scientific and Technological Development.
Resumo: Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic, chondrogenic and adipogenic lineage; and submitted to viral challenge. The proliferation of goat umbilical cord cells was fast and cell monolayers formed after 15 days. These cells exhibited morphology, immunophenotype, growth characteristics, and lineage differentiation potential similar to mesenchymal stem cells of other origins. The goat umbilical cord derived cells stained positive for vimentin and CD90, but negative for cytokeratin, CD34 and CD105 markers. Syncytia and cell lysis were observed in cell monolayers infected by CAEV-Cork and MVV-K1514, showing that the cells are permissive to small ruminant lentivirus infection in vitro. These data demonstrate the proliferative competence of cells derived from goat umbilical cords and provide a sound basis for future research to standardize this cell lineage.
Descritores: Cordão Umbilical/citologia
Lentivirus/fisiologia
Células-Tronco Mesenquimais/virologia
-Osteogênese
Replicação Viral
Técnicas In Vitro
Cabras
Biomarcadores
Diferenciação Celular
Células Cultivadas
Imunofenotipagem
Técnicas de Cultura de Células
Condrogênese
Efeito Citopatogênico Viral
Adipogenia
Células-Tronco Mesenquimais/citologia
Células-Tronco Mesenquimais/metabolismo
Células-Tronco Mesenquimais/patologia
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-713677
Autor: Borgonovo, Tamara; Vaz, Isadora May; Senegaglia, Alexandra Cristina; Rebelatto, Carmen Lucia Kuniyoshi; Brofman, Paulo Roberto Slud.
Título: Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center
Fonte: Rev. bras. hematol. hemoter;36(3):202-207, May-Jun/2014. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa; . Conselho Nacional de Pesquisa; . Conselho Nacional de Pesquisa.
Resumo: OBJECTIVE: To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center. METHODS: A retrospective study was carried out of 21 candidates for cell therapy. After the isolation of bone marrow mononuclear cells by density gradient, mesenchymal stem cells were cultivated and expanded at least until the second passage. Cytogenetic analyses were performed before and after cell expansion (62 samples) using G-banded karyotyping. RESULTS: All the samples analyzed, before and after cell expansion, had normal karyotypes, showing no clonal chromosomal changes. Signs of chromosomal instability were observed in 11 out of 21 patients (52%). From a total of 910 analyzed metaphases, five chromatid gaps, six chromatid breaks and 14 tetraploid cells were detected giving as total of 25 metaphases with chromosome damage (2.75%). CONCLUSION: The absence of clonal chromosomal aberrations in our results for G-banded karyotyping shows the maintenance of chromosomal stability of bone marrow-derived mesenchymal stem cells until the second passage; however, signs of chromosomal instability such as chromatid gaps, chromosome breaks and tetraploidy indicate that the long-term cultivation of these cells can provide an intermediate step for tumorigenesis. .
Descritores: Citogenética
Instabilidade Cromossômica
Células-Tronco Mesenquimais
Cariotipagem
Limites: Humanos
Masculino
Feminino
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-792596
Autor: ABUNA, Rodrigo Paolo Flores; STRINGHETTA-GARCIA, Camila Tami; FIORI, Leonardo Pimentel; DORNELLES, Rita Cassia Menegati; ROSA, Adalberto Luiz; BELOTI, Marcio Mateus.
Título: Aging impairs osteoblast differentiation of mesenchymal stem cells grown on titanium by favoring adipogenesis
Fonte: J. appl. oral sci;24(4):376-382, July-Aug. 2016. graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . National Council for Scientific and Technological Development.
Resumo: ABSTRACT Aging negatively affects bone/titanium implant interactions. Our hypothesis is that the unbalance between osteogenesis and adipogenesis induced by aging may be involved in this phenomenon. Objective We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) from young and aged rats cultured on Ti. Material and Methods Bone marrow MSCs derived from 1-month and 21-month rats were cultured on Ti discs under osteogenic conditions for periods of up to 21 days and osteoblast and adipocyte markers were evaluated. Results Cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of RUNX2, osterix, ALP, bone sialoprotein, osteopontin, and osteocalcin were reduced in cultures of 21-month rats compared with 1-month rats grown on Ti. Gene expression of PPAR-γ , adipocyte protein 2, and resistin and lipid accumulation were increased in cultures of 21-month rats compared with 1-month rats grown on the same conditions. Conclusions These results indicate that the lower osteogenic potential of MSCs derived from aged rats compared with young rats goes along with the higher adipogenic potential in cultures grown on Ti surface. This unbalance between osteoblast and adipocyte differentiation should be considered in dental implant therapy to the elderly population.
Descritores: Osteoblastos/fisiologia
Titânio/química
Envelhecimento/fisiologia
Implantes Dentários
Adipogenia/fisiologia
Células-Tronco Mesenquimais/fisiologia
-Osteogênese/fisiologia
Propriedades de Superfície
Expressão Gênica
Células Cultivadas
Fatores Etários
Proliferação de Células/fisiologia
Fosfatase Alcalina/análise
Reação em Cadeia da Polimerase em Tempo Real
Lipídeos/análise
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-777353
Autor: Mendi, Ayşegül; Köse, Sevil; Uçkan, Duygu; Akca, Gülçin; Yilmaz, Derviş; Aral, Levent; Gültekin, Sibel Elif; Eroğlu, Tamer; Kiliç, Emine; Uçkan, Sina.
Título: Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation
Fonte: J. appl. oral sci;24(1):67-75, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Projeto: TUBITAK; . TUBITAK; . TUBITAK.
Resumo: ABSTRACT An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
Descritores: Interleucina-8/análise
Porphyromonas gingivalis/imunologia
Probióticos/farmacologia
Lactobacillus rhamnosus/fisiologia
Células-Tronco Mesenquimais/microbiologia
-Periodontite/microbiologia
Aderência Bacteriana/imunologia
Ensaio de Imunoadsorção Enzimática
Células Cultivadas
Interleucina-8/imunologia
Interferon gama/análise
Interferon gama/imunologia
Interleucina-10
Estatísticas não Paramétricas
Receptor 4 Toll-Like/análise
Receptor 4 Toll-Like/imunologia
Citometria de Fluxo
Imunidade Inata
Limites: Humanos
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1038673
Autor: Rocha, A. R; Leite, Y. K. C; Silva, A. S; Conde Júnior, A. M; Costa, C. R. M; Silva, G. C; Bezerra, D. O; Cavalcante, M. M. A. S; Feitosa, M. L. T; Argôlo Neto, N. M; Serakides, R; Carvalho, M. A. M.
Título: Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha) / Imunofenotipagem, testes de plasticidade e marcação com nanocristais de céluas-tronco derivadas de tecido adiposo em cutias
Fonte: Arq. bras. med. vet. zootec. (Online);71(5):1571-1581, set.-out. 2019. graf, ilus.
Idioma: en.
Projeto: CNPq.
Resumo: There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three lineages, population CD90 + and CD45 - expression, which issued high red fluorescence after the tagging with fluorescent nanocrystals by different paths and cryopreserved for future use. It is possible to conclude that mesenchymal stem cells from agouti adipose tissue have biological characteristics and in vitro behavior that demonstrate its potential for use in clinical tests.(AU)

Há um interesse crescente no estudo das células estaminais mesenquimais, não especializadas, pois ainda existem algumas discussões sobre seu comportamento in vitro. A medicina regenerativa é uma ciência em fase de crescimento que desenvolve tratamentos como terapia celular utilizando células estaminais somáticas. Em vários estudos, o tecido adiposo é apresentado como uma fonte de células adultas multipotentes que tem várias vantagens em relação a outras fontes de tecido. Este estudo teve como objetivo caracterizar e avaliar a marcação de células estaminais mesenquimais do tecido adiposo de cutias (Dasyprocta prymnolopha) com nanocristais intracitoplasmáticos fluorescentes. Observaram-se células fibroblásticas, aderentes ao plástico, com autorrenovação prolongada, capacidade de formar colônias, diferenciação em três linhagens, população CD90 + e expressão CD45, que emitiram alta fluorescência vermelha após a marcação com nanocristais fluorescentes por diferentes vias, e criopreservadas para uso futuro. É possível concluir que as células estaminais mesenquimais do tecido adiposo de cutias têm características biológicas e comportamentos in vitro que demonstram seu potencial para uso em testes clínicos.(AU)
Descritores: Tecido Adiposo/citologia
Imunofenotipagem/veterinária
Medicina Regenerativa/métodos
Nanopartículas
Células-Tronco Mesenquimais
Dasyproctidae/genética
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-893174
Autor: Hajian Monfared, Mahdieh; Akbari, Mohammad; kashani, Iraj Ragerdi; Solhjoo, Somayeh; Tooli, Heidar; Omidi, Ameneh; Aliakbari, Freshteh; Ijaz, Sahar; Mokhtari, Tahmineh; Rastegar, Tayebeh.
Título: Inductive role of sustentacular cells (Sertoli cells) conditioned medium on bone marrow derived mesenchymal stem cells / Papel inductivo del medio acondicionado de células sustentaculares (células de Sertoli) en las células madre mesenquimales derivadas de la médula ósea
Fonte: Int. j. morphol;35(4):1597-1606, Dec. 2017. tab, graf.
Idioma: es.
Projeto: Tehran University of Medical Sciences.
Resumo: RESUMEN: Las células madre de la línea germinal masculina son factores clave para la espermatogénesis masculina y la fertilidad. Las células sustentaculares (células de Sertoli) como células somáticas juegan un papel fundamental en la creación de un microambiente esencial para la auto-renovación y diferenciación de las células de la línea germinal masculina. Las células madre mesenquimales son reconocidas como células auto-renovables y multipotentes capaces de diferenciarse en múltiples tipos de células. La generación de células germinales masculinas a partir de células madre mesenquimales puede proporcionar un método terapéutico para tratar la infertilidad masculina. En este estudio, las células mesenquimales derivadas de la médula ósea (BMMSCs) se recuperaron de la médula ósea de ratones de 6-8 semanas de edad del Instituto de Investigación Médico Naval (NMRI). En el estudio se aislaron las células sustentaculares y se enrriquecieron usando placas revestidas con lectina. Se obtuvo el medio de condición celular después de diferentes intervalos de tiempo. Posteriormente se cultivaron las BMMSC con diferentes concentraciones de SCCM y medio de Eagle modificado por Dulbecco (DMEM) en diversos momentos. Se evaluaron marcadores específicos de células de línea germinal usando la reacción en cadena de polimerasa transcriptasa inversa (RT-PCR) e inmunocitoquímica. Los resultados mostraron que las BMMSCs cultivadas con SCCM durante 48h exhibieron transcritos específicos de línea germinal (Mvh, Iid4, piwil2) (p <0,05) y marcadores (Mvh, Scp3). Nuestros resultados indican que el cultivo de BMMSCs con SCCM puede conducir a la diferenciación efectiva de BMMSCs en células germinales y proporcionar una estrategia de tratamiento para la infertilidad masculina.

SUMMARY: Male germ line stem cells are key factors for male spermatogenesis and fertility. Sustentacular cells (Sertoli cells) as somatic cells play a pivotal role in creating essential microenvironment for the self-renewal and differentiation of the male germ line cells. Mesenchymal stem cells are recognized as self-renewing and multipotent cells able to differentiate into multiple cell types. The generation of male germ cells from mesenchymal stem cells may provide a therapeutic method to treat male infertility. In this study, Bone marrow derived mesenchymal cells (BMMSCs) were retrieved from the bone marrow of 6-8-week old Naval Medical Research Institute (NMRI) mice. Sustentacular cells (Sertoli cells) were isolated and made rich using lectin coated plates. Sustentacular cell condition medium (SCCM) was collected after different time intervals. Then the BMMSCs were cultured with different concentration of SCCM and Dulbecco's Modified Eagle's medium (DMEM) at various times. Specific markers of Germ line cells were evaluated by using Reverse transcriptase polymerase chain reaction (RT-PCR) and immunocytochemistry. The results showed that BMMSCs cultured with SCCM for 48h exhibited germ line specific transcripts (Mvh, Iid4, piwil2) (p< 0.05) and markers (Mvh, Scp3). Our findings represent that culturing BMMSCs with SCCM may lead to effective differentiation of BMMSCs into germline cells and provide a treatment strategy for male infertility.
Descritores: Células de Sertoli/citologia
Células-Tronco Mesenquimais/citologia
-Células de Sertoli/ultraestrutura
Testículo/citologia
Medula Óssea
Imuno-Histoquímica
Diferenciação Celular
Meios de Cultivo Condicionados
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Citometria de Fluxo
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-839523
Autor: ALBIERO, Mayra Laino; AMORIM, Bruna Rabelo; CASATI, Márcio Zaffalon; SALLUM, Enilson Antonio; NOCITI JUNIOR, Francisco Humberto; SILVÉRIO, Karina Gonzales.
Título: Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides
Fonte: Braz. oral res. (Online);31:e17, 2017. tab, graf.
Idioma: en.
Projeto: São Paulo State Research Foundation.
Resumo: Abstract Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1β, TNF-α and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1β and TNF-α, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs.
Descritores: Osteogênese/efeitos dos fármacos
Ligamento Periodontal/citologia
Lipopolissacarídeos/toxicidade
Porphyromonas gingivalis
Células-Tronco Mesenquimais/efeitos dos fármacos
-Fatores de Tempo
Expressão Gênica
Osteocalcina/análise
Diferenciação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Fator de Necrose Tumoral alfa/análise
Estatísticas não Paramétricas
Proliferação de Células/efeitos dos fármacos
Fosfatase Alcalina/análise
Fator 3 de Transcrição de Octâmero/análise
Receptores Toll-Like/análise
Subunidade alfa 1 de Fator de Ligação ao Core/análise
Interleucina-1beta/análise
Células-Tronco Mesenquimais/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-736040
Autor: Maciel, Bruno B; Rebelatto, Carmen L. K; Brofman, Paulo R. S; Brito, Harald F. V; Patricio, Lia F. L; Cruz, Marúcia A; Locatelli-Dittrich, Rosangela.
Título: Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture / Morfologia e morfometria das células-tronco mesenquimais isoladas de medula óssea de gato
Fonte: Pesqui. vet. bras = Braz. j. vet. res;34(11):1127-1134, nov. 2014. ilus, tab, graf.
Idioma: en.
Resumo: Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for treatment of a variety of different diseases in human and veterinary medicine. Stem cells have been isolated from feline bone marrow, however, very few data exist about the morphology of these cells and no data were found about the morphometry of feline bone marrow-derived MSCs (BM-MSCs). The objectives of this study were the isolation, growth evaluation, differentiation potential and characterization of feline BM-MSCs by their morphological and morphometric characteristics. in vitro differentiation assays were conducted to confirm the multipotency of feline MSC, as assessed by their ability to differentiate into three cell lineages (osteoblasts, chondrocytes, and adipocytes). To evaluate morphological and morphometric characteristics the cells are maintained in culture. Cells were observed with light microscope, with association of dyes, and they were measured at 24, 48, 72 and 120h of culture (P1 and P3). The non-parametric ANOVA test for independent samples was performed and the means were compared by Tukey's test. On average, the number of mononuclear cells obtained was 12.29 (±6.05x106) cells/mL of bone marrow. Morphologically, BM-MSCs were long and fusiforms, and squamous with abundant cytoplasm. In the morphometric study of the cells, it was observed a significant increase in average length of cells during the first passage. The cell lengths were 106.97±38.16µm and 177.91±71.61µm, respectively, at first and third passages (24 h). The cell widths were 30.79±16.75 µm and 40.18±20.46µm, respectively, at first and third passages (24 h).The nucleus length of the feline BM-MSCs at P1 increased from 16.28µm (24h) to 21.29µm (120h). However, at P3, the nucleus length was 26.35µm (24h) and 25.22µm (120h). This information could be important for future application and use of feline BM-MSCs.(AU)

As células tronco mesenquimais são utilizadas na terapia de várias doenças na medicina humana e veterinária. As células tronco foram isoladas da medula óssea de gato, entretanto, existem poucos dados referentes a morfologia e não existem informações sobre a morfometria das células tronco isoladas da medula óssea. Os objetivos do presente estudo foram o isolamento, avaliação do crescimento, potencial de diferenciação e caracterização morfológica e morfométrica das células mesenquimais de gato isoladas de medula óssea. A diferenciação in vitro foi realizada para confirmar a multipotencialidade das células mesenquimais de gato (diferenciação em osteoblastos, condrócitos, adipócitos). As células mesenquimais foram mantidas em cultivo para avaliações morfológica e morfométrica. As células foram coradas e observadas em microscopia ótica. As mensurações foram realizadas com 24, 48, 72 e 120h de cultura (primeira e terceira passagens). O teste não paramétrico ANOVA foi utilizado e as médias foram comparadas pelo teste de Tukey. O número médio de células mononucleares obtido foi de 12,29 (±6,05x106) células/mL de medula óssea. As células mesenquimais são longas e fusiformes, e escamosas com citoplasma abundante. No estudo morfométrico, observou-se aumento no comprimento médio das células durante a primeira passagem. As medidas de comprimento das células foram: 106,97±38,16µm e 177,91±71,61µm, respectivamente, na primeira e terceira passagens (24 horas). As medidas de largura das células foram: 30,79±16,75 µm e 40,18±20,46 µm, respectivamente, na primeira e terceira passagens (24 horas). O comprimento do núcleo na primeira passagem aumentou de 16,28µm (24h) para 21,29µm (120h) e na terceira passagem foi de 26,35µm (24h) para 25,22µm (120h). As informações são importantes para futuras aplicações e uso da célula mesenquimal de gato.(AU)
Descritores: Medula Óssea/anatomia & histologia
Células-Tronco Mesenquimais/citologia
Limites: Animais
Gatos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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