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Id: biblio-1095234
Autor: Rawat, Garima; Urs, Aadithya B; Chakravarti, Anita; Kumar, Priya.
Título: DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay: a comparison with blood leukocytes
Fonte: Braz. j. oral sci;18:e191430, jan.-dez. 2019. ilus.
Idioma: en.
Resumo: Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of DNA damage; lymphocytesare the most commonly used cells for performing comet assay. Utilisation of buccal epithelial cells in comet assay can be a minimally invasive and rapid method. The present study compared the efficacy of comet assay in assessing DNA damage in buccal cells over peripheral blood leucocytes (PBLs) in oral potentially malignant and malignant disorders. Methods: The study included fifty five patients each of Leukoplakia, Oral Submucous Fibrosis (OSMF) and OSCC along with fifty five healthy individuals as control. Buccal epithelial cells were collected from all the selected subjects. DNA damage was evaluated bymeasuring the mean tail length (µm). Results: A significantly increased mean tail length (µm) and higher DNA damage were found in OSCC (26.1096 + 1.84355) and there was a progressive stepwise increase in mean tail length from control(8.4982 + 0.93307) to PMD [leukoplakia (14.6105 + 0.71857); OSMF (12.5009 + 1.12694)] to OSCC.The mean tail length in different habit groups was greater than controls, though no significant difference was noted between habit groups. The mean tail length of buccal cells was significantly greater than the mean tail length of PBLs in all study groups and controls. Conclusion: Hence, use of comet assay on buccal epithelial cells can prove to be beneficiary for evaluation of DNA damage
Descritores: Dano ao DNA
Neoplasias Bucais
Ensaio Cometa
Células Epiteliais
Leucócitos
Limites: Humanos
Masculino
Feminino
Responsável: BR218.1 - Biblioteca Carlos Henrique Robertson Liberalli


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Cuppari, Lilian
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Id: biblio-975911
Autor: Andrade, Laila Santos de; Dalboni, Maria Aparecida; Carvalho, José Tarcisio Giffoni de; Grabulosa, Caren Cristina; Pereira, Natalia Barros Ferreira; Aoike, Danilo Takashi; Cuppari, Lilian.
Título: In vitro effect of uremic serum on barrier function and inflammation in human colonocytes / Efeito in vitro do soro urêmico sobre a função de barreira e inflamação em colonócitos humanos
Fonte: J. bras. nefrol;40(3):217-224, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: ABSTRACT Introduction: In chronic kidney disease (CKD), it has been suggested that alterations within the gut are associated with an inflammatory state and uremic toxicity. Studies suggest that uremia may impair the function of the intestinal barrier via the promotion of increased intestinal permeability. To understand the mechanisms that are involved in intestinal barrier damage in the setting of uremia, we evaluated the in vitro effect of uremic serum on transepithelial electrical resistance (TER), inflammation, and apoptosis in intestinal epithelial cells (T84). Methods: Pools of serum from healthy individuals, patients not on dialysis, and patients on hemodialysis (Pre-HD and Post-HD) were prepared. T84 cells were incubated for 24 h in medium, of which 10% consisted of the pooled serum from each group. After incubation, the TER was measured and the following parameters were determined by flow cytometry: expression of toll-like receptors (TLRs), production of reactive oxygen species (ROS), and apoptosis. The level of IL-6 in the culture supernatant was determined by ELISA. Results: No difference was observed among the groups with respect to TER, apoptosis, and ROS or the expression of TLR-2, TLR-4, and TLR-9. IL-6 secretion was higher (p < 0.001) in cells that were incubated with pre- and post-HD serum. Conclusion: The results that were obtained from this model suggest that uremic serum per se does not seem to impair the integrity of intestinal epithelial cells. The increased IL-6 secretion by cells that were incubated with HD serum suggests a potential effect of uremia in the intestinal inflammatory response.

RESUMO Introdução: Tem sido sugerido que na doença renal crônica (DRC) a uremia pode causar alterações intestinais, tais como modificações na microbiota e danos à barreira intestinal, e que estas possíveis alterações podem ter uma relação importante com o estado inflamatório e a toxicidade urêmica apresentadas por pacientes com DRC. Objetivos: Avaliar o efeito in vitro do soro urêmico sobre a permeabilidade da monocamada de células epiteliais do intestino, inflamação e apoptose. Métodos: Pools de soro foram preparados a partir de soros de indivíduos saudáveis, pacientes em tratamento conservador e em hemodiálise (Pré e Pós-HD). As células T84 foram incubadas por 24 horas com os diferentes pools. Em seguida a TER foi medida e as células foram submetidas às seguintes análises: apoptose, produção de espécies reativas de oxigênio (EROs) e expressão de receptores toll-like (TLR) por citometria de fluxo e detecção de IL-6 no sobrenadante da cultura por ELISA. Resultados: Não foram encontradas diferenças, entre os grupos, com relação a TER, apoptose, EROs e expressão de TLR-2, TLR-4 e TLR-9. Já a secreção de IL-6 foi maior (p < 0,001) pelas células incubadas com soro pré-HD e pós-HD. Conclusão: Os resultados obtidos a partir deste modelo sugerem que a uremia per se parece não comprometer a integridade das células epiteliais do intestino. O aumento da secreção de IL-6 pelas células incubadas com soro HD (pré e pós) sugere um potencial efeito da uremia sobre a resposta inflamatória intestinal.
Descritores: Fenômenos Fisiológicos Sanguíneos
Células Epiteliais/fisiologia
Inflamação/etiologia
-Uremia/sangue
Células Cultivadas
Colo/citologia
Insuficiência Renal Crônica/sangue
Mucosa Intestinal/citologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-1019265
Autor: Shen, Yezhou; Yu, Jiaoyang; Jing, Yunyan; Zhang, Jian.
Título: MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model
Fonte: Acta cir. bras;34(6):e201900602, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Purpose To investigate the role and related mechanisms of miR-106a in sepsis-induced AKI. Methods Serum from sepsis and healthy patients was collected, sepsis mouse model was established by cecal ligation and puncture (CLP). TCMK-1 cells were treated with lipopolysaccharide (LPS) and transfected with THBS2-small interfering RNA (siTHBS2), miR-106a inhibitor, miR-106a mimics and their negative controls (NCs). The expression of miR-106a, thrombospondin 2 (THBS2), Bax, cleaved caspase-3 and Bcl-2, cell viability, relative caspase-3 activity and TNF-α, IL-1β, IL-6 content were respectively detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, Cell Counting Kit-8 (CCK-8) and enzyme linked immunosorbent assay (ELISA). The relationship between miR-106a and THBS2 was confirmed by dual luciferase reporter assay. Results MiR-106a was up-regulated in serum of sepsis patients, CLP-induced mice models and LPS-induced TCMK-1 cells. LPS reduced cell viability and Bcl-2 expression, and increased caspase-3 activity, Bax expression, the content of TNF-α, IL-1β, IL-6. THBS2 was a target of miR-106a. The decreases of caspase-3 activity, TNF-α, IL-1β, IL-6, Bax expression and the increases of cell viability, Bcl-2 expression caused by miR-106a knockdown were reversed when THBS2 silencing in LPS-stimulated TCMK-1 cells. Conclusion MiR-106a aggravated LPS-induced inflammation and apoptosis of TCMK-1 cells via regulating THBS2 expression.
Descritores: Sepse/patologia
Trombospondinas/farmacologia
MicroRNAs/metabolismo
Células Epiteliais/patologia
Lesão Renal Aguda/metabolismo
Rim/citologia
-Ensaio de Imunoadsorção Enzimática
Transfecção
Estudos de Casos e Controles
Células Cultivadas
Citocinas/metabolismo
Apoptose
Sepse/metabolismo
Modelos Animais de Doenças
Lesão Renal Aguda/patologia
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Animais
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1050177
Autor: Medeiros, Letícia Campos.
Título: Interação de Toxoplasma gondii e células epiteliais intestinais de felinos in vitro / Interaction of Toxoplasma gondii and feline intestinal epithelial cells in vitro.
Fonte: Rio de Janeiro; s.n; 2019. 105 p. ilus.
Idioma: pt.
Tese: Apresentada a Instituto Oswaldo Cruz para obtenção do grau de Mestre.
Resumo: Toxoplasma gondii é um protozoário parasito intracelular obrigatório disseminado pelo mundo, capaz de infectar células nucleadas de todos os animais de sangue quente: aves e mamíferos, incluindo o homem. Com grande promiscuidade em relação aos seus hospedeiros intermediários, T. gondii é altamente específico quanto à etapa sexuada de seu ciclo intestinal no seu hospedeiro definitivo, o felino. Apesar da importância do ciclo enteroepitelial para o ambiente, há grandes lacunas no entendimento deste ciclo. Grande parte do conhecimento atual é produto do desenvolvimento de pesquisas in vivo em décadas passadas. Hoje a eutanásia de gatos para fins científicos é praticamente proibida e como não existem linhagens contínuas de enterócitos de felinos disponíveis comercialmente, dificulta a realização de pesquisas in vitro. Assim, neste estudo aplicamos o modelo de cultura primária de células epiteliais intestinais de felinos (CEIF) como ferramenta para reproduzir o ciclo sexuado in vitro e identificar os estágios enteroepiteliais do parasito

O monitoramento in situ das células epiteliais intestinais de felino mostrou a reprodutibilidade do fenótipo de enterócitos in vitro e sua característica ultraestrutral. Nas nossas condições experimentais, as monobras na variação da proporção parasito:célula hospedeira, confimou que a relação 1:20 foi determinante para o estabelecimento tanto do ciclo lítico e da cistogênese quanto da indução de formas semelhantes a esquizontes, como revelados por microscopia de luz e eletrônica de transmissão. Foram identificados esquizontes dos tipos C, D e E, apresentando características morfológicas similares às previamente descritas na literatura com base na análise de intestino de gatos infectados experimentalmente por T. gondii. Estes dados indicam que as CEIF simulam in vitro o microambiente celular natural intestinal do felino que favorece o desenvolvimento da esquizogonia. Esta metodologia abre novas perspectivas para investigação de aspectos biológicos e moleculares envolvidos no ciclo entérico de T. gondii in vitro. Além disso, possibilita agregar conhecimento para o desenvolvimento de novas estratégias direcionadas à intervenção da transmissão do parasito, visando interferir numa das principais vias pelas quais a toxoplasmose se propaga, pelas fezes de felinos contaminando o meio ambiente. (AU)
Descritores: Toxoplasma
Toxoplasmose
Células Epiteliais
Cultura Primária de Células
Limites: Animais
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas


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Id: biblio-887184
Autor: Oliveira, Cristiano Claudino; Nóbrega, Vinicius Cardoso; Marques, Mariângela Esther Alencar.
Título: Lymphoepithelioma-like carcinoma of the skin
Fonte: An. bras. dermatol;93(2):256-258, Mar.-Apr. 2018. graf.
Idioma: en.
Resumo: Abstract: Primary cutaneous lymphoepithelioma-like carcinoma is a rare disease with low metastatic potential. Its morphologic and pathological features are similar to those of nasopharyngeal lymphoepithelial carcinoma. We report the case of a 60-year-old man with an infrapalpebral pearly papule, measuring 0.6 cm in diameter. The lesion was excised with a clinical hypothesis of basal cell carcinoma or squamous cell carcinoma. Histopathological analysis revealed a malignant neoplasm with syncytial arrangement of cells with vesicular nuclei, associated with dense lymphocytic infiltrate. Immunohistochemistry revealed cytokeratin-positive cells (AE1/AE3) and p63 protein, indicating epithelial histogenesis and squamous differentiation. A negative Epstein-Barr virus test result was achieved by immunohistochemistry. Primary lymphoepithelioma-like carcinoma of the skin is a differential diagnosis of lesions with prominent inflammatory infiltrates.
Descritores: Neoplasias Cutâneas/patologia
Carcinoma/patologia
-Biópsia
Imuno-Histoquímica
Carcinoma Basocelular/patologia
Carcinoma de Células Escamosas/patologia
Diagnóstico Diferencial
Células Epiteliais/patologia
Limites: Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-839393
Autor: Yu, Yi; Chang, De; Xu, Huiwen; Zhang, Xuelin; Pan, Lei; Xu, Chou; Huang, Bing; Zhou, Hong; Li, Jia; Guo, Jun; Liu, Changting.
Título: The virulence of Streptococcus pneumoniae partially depends on dprA
Fonte: Braz. j. microbiol;48(2):225-231, April.-June 2017. graf.
Idioma: en.
Projeto: National Basic Research Program of China; . National Major Scientific and Technological Special Project for \"Significant New Drugs Development\"; . National Natural Science Foundation of China; . Basic Research Program of General Hospital of Chinese People's Armed Police Forces.
Resumo: Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study was to characterize the effects of the pneumococcal dprA gene on the pathogenesis of S. pneumoniae. To construct a dprA-deficient pneumococcal strain, the dprA gene of the S. pneumoniae strain D39 was inactivated. The virulence of this dprA-deficient strain, designated ΔD39, was compared with that of the wild-type strain by evaluating their respective capabilities to adhere to human pulmonary epithelial cells (PEC-A549) and by analyzing their choline-binding protein expression levels. In addition, the expression profiles of genes associated with virulence and host survival assays were also conducted with the mutant and the wild-type strain. Our results indicate that the capability of ΔD39 to adhere to the PEC-A549 airway cells was significantly lower (p < 0.01) compared with D39. Additionally, the 100-KD choline-binding protein was not detected in ΔD39. The addition of competence-stimulating peptide (CSP) lead to a significantly reduction of psaA mRNA expression in the dprA-deficient mutant and an increased level of psaA transcripts in the wild-type strain (p < 0.01). The median survival time of mice intraperitoneally infected with ΔD39 was significantly higher (p < 0.01) than that of mice infected with D39. The results of this study suggest that DprA has a significant effect on virulence characteristics of S. pneumoniae by influencing the expression of choline-binding protein and PsaA.
Descritores: Infecções Pneumocócicas/patologia
Streptococcus pneumoniae/patogenicidade
Proteínas de Bactérias/metabolismo
Aderência Bacteriana
Fatores de Virulência/análise
Proteínas de Membrana/metabolismo
-Infecções Pneumocócicas/microbiologia
Streptococcus pneumoniae/genética
Proteínas de Bactérias/análise
Proteínas de Bactérias/genética
Análise de Sobrevida
Linhagem Celular
Fatores de Virulência/genética
Modelos Animais de Doenças
Células Epiteliais/microbiologia
Técnicas de Inativação de Genes
Proteínas de Membrana/genética
Camundongos
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: lil-728291
Autor: Díaz-Peña, Roberto; Castro-Santos, Patricia.
Título: Histological changes implicated in metastasis / Cambios histológicos implicados en metástasis
Fonte: Int. j. morphol;32(3):935-941, Sept. 2014. ilus.
Idioma: en.
Projeto: Universidad Autónoma de Chile.
Resumo: The process of malignancy emergence is associated with the acquisition of the capacity to invade other tissues. Several different biological processes have been described as involved in this process. Specifically, epithelial mesenchymal transition (EMT), a mechanism associated with embryogenesis and wound repair but also with mobility acquisition, is one of the concerned processes. In EMT an epithelial cell loses its epithelial characteristics, its junctions with neighbor cells and with the basal lamina and acquires mobility and mesenchymal characteristics. Also, factors of the tumor microenvironment have been described as involved. Tumor presence triggers a response in the surrounding tissue known as reactive stromal. It shows particular characteristics similar to those found in wound healing stroma: an increase of the fibroblast number and enhancing of the capillary density. The notable difference is the chronicity in the tumoral process. Of a high relevance seems to be the role of activated macrophages with a characteristic phenotype. Finally, cancer associated fibroblasts (CAF) are a type of cells found in tumors, developed from local tissue or possibly from bone marrow. CAF characteristically show a distinct morphology and secrete a high number of metalloproteases allowing tumoral cells advance through the tissue. Additionally, CAF have a direct effect on the survival of the epithelial cells. The three processes are interrelated and metastasis is probably caused by the effect of all of them and probably by other additional factors.

El desarrollo de malignidad está asociado con la adquisición de la capacidad de invadir otros tejidos. Varios procesos diferentes han sido asociados con la aparición de metástasis. Concretamente, la transición epitelio mesénquima (TEM), un mecanismo asociado con embriogénesis y reparación de heridas pero también con adquisición de movilidad, es uno de ellos. En la TEM, una célula epitelial pierde sus características epiteliales, sus uniones con las células vecinas y con la lámina basal y adquiere movilidad y características mesenquemáticas. También han sido asociados factores del microambiente del tumor. La presencia del tumor produce una respuesta en el tejido que lo rodea descrito como estroma reactivo. Sus características son similares a las del estroma de las heridas en proceso de curación: un incremento del número de fibroblastos y un aumento de la densidad de capilares. La gran diferencia es la cronicidad del proceso tumoral. De gran relevancia es el papel de los macrófagos activados que muestran un fenotipo característico. Finalmente, los fibroblastos asociados a cáncer (FAC) son un tipo de células encontradas en tumores, que se desarrollan a partir del tejido local o quizá de la médula ósea. Los FAC, de modo característico muestran una morfología diferente y secretan una gran cantidad de metaloproteasas permitiendo a la célula tumoral avanzar a través del tejido. Además, los FAC ejercen un efecto directo sobre la supervivencia de las células epiteliales. Los tres procesos están interrelacionados y la metástasis es causada probablemente por el efecto de todos ellos y probablemente por otros factores adicionales.
Descritores: Células Epiteliais/patologia
Fibroblastos Associados a Câncer/patologia
Metástase Neoplásica/patologia
-Transição Epitelial-Mesenquimal
Invasividade Neoplásica
Neoplasias/patologia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1007341
Autor: Valdés, Claudio; Valenzuela, Beatriz; Modak, Brenda.
Título: Evaluation in vitro of proliferative activity of epithelial cells by flavonoid 3-O-methylgalangine and terpenenic derivative Filifolinone / Evaluación in vitro de la actividad proliferative de células epiteliales del flavonoide 3-O-metilgalangina y el derivado terpénico Filifolinona
Fonte: Bol. latinoam. Caribe plantas med. aromát;17(6):575-582, nov. 2018. ilus.
Idioma: en.
Projeto: Fondecyt.
Resumo: The skin is the largest organ of the human body and its main function is to protect it from the external environment. It is exposed to injuries that require a rapid healing process to recover its functionality. Microorganisms inhabit the skin, which makes up the normal microbial flora, but in situations of injury they can cause infections that slow down the regeneration process. Therefore, there is a great interest in the development of alternative methods to accelerate the regeneration process and prevent infections. In this work, the efficacy of flavonoid 3-O-methylgalangine and the terpenic derivative Filifolinone and its mixtures, isolated from plants of the genus Heliotropium, on the stimulation of cell proliferation was evaluated. The results showed that the mixtures stimulated proliferation and migration in MA104 cells mainly due to the presence of Filifolinone, that together with the known antibacterial activity of 3-O-methylgalangine, opens new alternatives for the use of natural compounds in healing processes.

La piel es el órgano más grande del cuerpo humano y su función principal es protegerla del entorno externo. Está expuesta a lesiones que requieren un proceso de curación rápido para recuperar su funcionalidad. Los microorganismos que habitan en la piel, constituyen la flora microbiana normal, pero en situaciones de lesión pueden causar infecciones que retardan el proceso de regeneración. Por lo tanto, existe un gran interés en el desarrollo de métodos alternativos para acelerar el proceso de regeneración y prevenir infecciones. En este trabajo, se evaluó la eficacia del flavonoide 3-O-metilgalangina y el derivado terpénico Filifolinona y sus mezclas, aisladas de plantas del género Heliotropium, en la estimulación de la proliferación celular. Los resultados mostraron que las mezclas estimularon la proliferación y la migración en las células MA104 debido principalmente a la presencia de Filifolinona, que junto con la actividad antibacteriana conocida de la 3-O-metilgalangina, abre nuevas alternativas para el uso de compuestos naturales en los procesos de curación.
Descritores: Terpenos/farmacologia
Flavonoides/farmacologia
Heliotropium
Proliferação de Células/efeitos dos fármacos
-Terpenos/química
Cicatrização
Flavonoides/química
Movimento Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Células Epiteliais/efeitos dos fármacos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-894838
Autor: Dias-Lopes, Geovane; Saboia-Vahia, Leonardo; Margotti, Eliane Trindade; Fernandes, Nilma de Souza; Castro, Cássia Luana de Faria; Oliveira Junior, Francisco Odencio; Peixoto, Juliana Figueiredo; Britto, Constança; Silva Filho, Fernando Costa e; Cuervo, Patricia; Jesus, José Batista de.
Título: Morphologic study of the effect of iron on pseudocyst formation in Trichomonas vaginalis and its interaction with human epithelial cells
Fonte: Mem. Inst. Oswaldo Cruz;112(10):664-673, Oct. 2017. graf.
Idioma: en.
Projeto: FAPERJ; . CNPq; . CNPq; . CNPq.
Resumo: BACKGROUND Trichomonas vaginalis is the aetiological agent of human trichomoniasis, which is one of the most prevalent sexually transmitted diseases in humans. Iron is an important element for the survival of this parasite and the colonisation of the host urogenital tract. OBJECTIVES In this study, we investigated the effects of iron on parasite proliferation in the dynamics of pseudocyst formation and morphologically characterised iron depletion-induced pseudocysts. METHODS We performed structural and ultrastructural analyses using light microscopy, scanning electron microscopy and transmission electron microscopy. FINDINGS It was observed that iron depletion (i) interrupts the proliferation of T. vaginalis, (ii) induces morphological changes in typical multiplicative trophozoites to spherical non-proliferative, non-motile pseudocysts, and (iii) induces the arrest of cell division at different stages of the cell cycle; (iv) iron is the fundamental element for the maintenance of typical trophozoite morphology; (v) pseudocysts induced by iron depletion are viable and reversible forms; and, finally, (vi) we demonstrated that pseudocysts induced by iron depletion are able to interact with human epithelial cells maintaining their spherical forms. MAIN CONCLUSIONS Together, these data suggest that pseudocysts could be induced as a response to iron nutritional stress and could have a potential role in the transmission and infection of T. vaginalis.
Descritores: Trichomonas vaginalis/efeitos dos fármacos
Trichomonas vaginalis/ultraestrutura
Microscopia Eletrônica de Varredura
Quelantes/farmacologia
Células Epiteliais/microbiologia
-Fatores de Tempo
Células HeLa
Ferro
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-889184
Autor: Llanco, Luis A; Nakano, Viviane; Moraes, Claudia T.P. de; Piazza, Roxane MF; Avila-Campos, Mario J.
Título: Adhesion and invasion of Clostridium perfringens type A into epithelial cells
Fonte: Braz. j. microbiol;48(4):764-768, Oct.-Dec. 2017. graf.
Idioma: en.
Projeto: CNPq; . FAPESP.
Resumo: ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of incubation and produced cell enlargement. None of the 22 strains harbored netB gene. All the six tpeL (+) gene strains were able to adhere to HEp-2 cells; however, only four of them (66.6%) were invasive. Thus, these results suggest that the presence of tpeL gene or TpeL toxin might be required for the adherence of bacteria to HEp-2 cells; however, it could not have any role in the invasion process.
Descritores: Doenças das Aves Domésticas/microbiologia
Aderência Bacteriana
Infecções por Clostridium/microbiologia
Infecções por Clostridium/veterinária
Clostridium perfringens/fisiologia
Células Epiteliais/microbiologia
-Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Células Vero
Chlorocebus aethiops
Galinhas
Clostridium perfringens/isolamento & purificação
Clostridium perfringens/genética
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde