Base de dados : LILACS
Pesquisa : A11.750 [Categoria DeCS]
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Id: biblio-1045993
Autor: Zeeshan, Nadia; Naz, Saher; Naz, Shumaila; Afroz, Amber; Zahur, Muzna; Zia, Safia.
Título: Heterologous expression and enhanced production of ß-1, 4-glucanase of Bacillus halodurans C-125 in Escherichia coli
Fonte: Electron. j. biotechnol;34:29-36, july. 2018. ilus, tab, graf.
Idioma: en.
Projeto: Higher Education Commission, Pakistan (HEC).
Resumo: Background: Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-ß-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study was aimed at enhancing the production of endo-1, 4-ß-glucanases (Egl) of Bacillus halodurans in Escherichia coli. Results: A putative Egl gene of Bacillus Halodurans was expressed in E. coli by cloning in pET 22b (+). On induction with isopropyl-b-D-1-thiogalactopyranoside, the enzyme expression reached upto ~20% of the cell protein producing 29.2 mg/liter culture. An increase in cell density to 12 in auto-inducing LB medium (absorbance at 600 nm) enhanced ß-glucanase production up to 5.4 fold. The molecular mass of the enzyme was determined to be 39 KDa, which is nearly the same as the calculated value. Protein sequence was analyzed by CDD, Pfam, I TASSER, COACH, PROCHECK Servers and putative amino acids involved in the formation of catalytic, substrate and metal binding domains were identified. Phylogenetic analysis of the ß-glucanases of B. halodurans was performed and position of Egl among other members of the genus Bacillus producing endo-glucanases was determined. Temperature and pH optima of the enzyme were found to be 60°C and 8.0, respectively, under the assay conditions. Conclusion: Production of endo-1, 4 ß-glucanase enzymes from B. halodurans increased several folds when cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 ß-glucanases from B. halodurans.
Descritores: Bacillus/enzimologia
Celulases/biossíntese
-Temperatura
Estabilidade Enzimática
Expressão Gênica
Parede Celular/enzimologia
Reação em Cadeia da Polimerase
Clonagem Molecular
Celulases/isolamento & purificação
Celulases/metabolismo
Escherichia coli/metabolismo
Células Vegetais/enzimologia
Concentração de Íons de Hidrogênio
Hidrólise
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1047981
Autor: Martínez, María Eugenia; Poirrier, Paola; Prüfer, Dirk; Schulze Gronover, Christian; Jorquera, Lorena; Ferrer, Perla; Díaz, Katy; Chamy, Rolando.
Título: Kinetics and modeling of cell growth for potential anthocyanin induction in cultures of Taraxacum officinale G.H. Weber ex Wiggers (Dandelion) in vitro
Fonte: Electron. j. biotechnol;36:15-23, nov. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Chilean Government project InnovaChile CORFO.
Resumo: Background: Taraxacum officinale G.H. Weber ex Wiggers is a wild plant used in folk medicine to treat several diseases owing to bioactive secondary metabolites present in its tissue. The accumulation of such molecules in plant cells can occur as a response against abiotic stress, but these metabolites are often deposited in low concentrations. For this reason, the use of a biotechnological approach to improve the yields of technologically interesting bioactive compounds such as anthocyanins is a compelling option. This work focuses on investigating the potential of in vitro T. officinale cultures as an anthocyanin source. Results: To demonstrate the suitability of anthocyanin induction and accumulation in calluses under specific conditions, anthocyanin was induced in the T. officinale callus. A specific medium of 5.5% sucrose supplemented with 6-benzylaminopurine /1-naphthaleneacetic acid in a 10:1 ratio was used to produce an anthocyanin yield of 1.23 mg g-1 fw. An in vitro dandelion callus line was established from this experiment. Five mathematical models were then used to objectively and predictably explain the growth of anthocyanin-induced calluses from T. officinale. Of these models, the Richards model offered the most suitable representation of anthocyanin callus growth in a solid medium and permitted the calculation of the corresponding kinetic parameters. Conclusions: The findings demonstrate the potential of an in vitro anthocyanin-induced callus line from T. officinale as an industrial anthocyanin source.
Descritores: Taraxacum/crescimento & desenvolvimento
Desenvolvimento Vegetal
Antocianinas/metabolismo
-Técnicas In Vitro
Cinética
Células Vegetais
Compostos Fitoquímicos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-940405
Autor: Bedi, Shella; Tanuja,; Vyas, S. P.
Título: A Handbook of aromatic and essential oil plants: cultivation, chemistry, processing and uses.
Fonte: Jodhpur; Agrobios (India); 2008. 598 p.
Idioma: en.
Descritores: Desenvolvimento Vegetal
Células Vegetais/classificação
Plantas/classificação
Responsável: BR1719.1 - Biblioteca do CPqRR
BR1719.1; 547.71, B412h, 2008. 010077



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