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Id: biblio-1128987
Autor: Santos, Leone José da Silva; Bittencourt, Marília da Silva Pereira; Terezan, Marilisa Lugon Ferreira; Rocha, Luiz Eduardo Monteiro Dias da.
Título: Fluido gengival e atividade de doença periodontal / Gingival fluid and periodontal disease activity
Fonte: Periodontia;30(3):189-194, 2020.
Idioma: pt.
Resumo: O fluxo do fluido sulcular gengival (FSG) é fisiológico, proveniente do plexo gengival dos vasos sanguíneos, subjacente ao revestimento epitelial. Aumenta em resposta à inflamação provocada pelo biofilme. Assim como a sua intensidade varia em função da inflamação gengival, observa-se mudanças na sua composição durante a progressão das doenças periodontais. É reconhecido como um grande reservatório de informações moleculares e como uma importante ferramenta de diagnóstico para as doenças periodontais. Sendo assim, o objetivo do deste estudo foi realizar uma revisão da literatura sobre FSG, estabelecer sua relação com a atividade de doença periodontal e sua importância como ferramenta de diagnóstico e prevenção das mesmas. Concluiu-se que os marcadores biológicos do fluido podem ajudar a determinar o risco e a gravidade das doenças periodontais. Essas análises, futuramente, poderão complementar o exame clínico, contribuindo na prevenção, diagnóstico e manejo personalizado de doenças periodontais. Porém, mais estudos devem ser realizados para a utilização rotineira desses métodos nos consultórios odontológicos (AU)

The gingival crevicular fluid flow (GCF) is physiological, that comes from the blood vessels of the gingival plexus, underlying the epithelial lining. It increases in response to the inflammation caused by the biofilm. As its intensity varies due the gingival inflammation degree, changes in its composition are observed during the progression of periodontal diseases. It is recognized as a large reservoir of information about molecules and as an important diagnostic tool for periodontal diseases. Therefore, the aim of this study was to conduct a review of the literature on GCF, to establish its relationship with the activity of periodontal disease and its importance as a tool for diagnosis and prevention. It has been concluded that biological fluid markers can help to determine the risk and severity of periodontal diseases. These analyzes, in the future, may complement the clinical examination, contributing to the prevention, diagnosis and personalized management of periodontal diseases. However, further studies should be performed for the routine use of these methods in dental offices. (AU)
Descritores: Doenças Periodontais
Periodontite
Líquido do Sulco Gengival
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR243.1 - Serviço Técnico de Biblioteca e Documentação


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Id: biblio-1089073
Autor: Odanaka, Hibiki; Obama, Takashi; Sawada, Naoko; Sugano, Marika; Itabe, Hiroyuki; Yamamoto, Matsuo.
Título: Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
Fonte: Biol. Res;53:03, 2020. tab, graf.
Idioma: en.
Projeto: JSPS KAKENHI.
Resumo: BACKGROUND: The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate the origin of pellicle proteins. RESULTS: The pellicle, gingival crevicular fluid, and saliva from the parotid gland or mixed gland were collected; subsequently, protein expression in samples from the respective individual was compared by SDS-PAGE and mass spectrometry. Following SDS-PAGE, proteins in the major bands were identified by mass spectrometry. The band pattern of pellicle proteins appeared different from those of gingival crevicular fluid, or saliva samples. Using mass spectrometry, 13 proteins in these samples were identified. The relative abundance of the proteins was quantitatively analyzed using mass spectrometry coupled with stable isotope labeling and by western blot. Cystatin S and α-amylase detected in pellicle were enriched in saliva samples, but not in gingival crevicular fluid, by western blot, and their abundance ratios were high in saliva and low in gingival crevicular fluid when analyzed by stable isotope labeling. Serotransferrin, however, was found only in the pellicle and gingival crevicular fluid by western blot and its abundance ratio was low in saliva. CONCLUSIONS: Our study revealed that the gingival crevicular fluid appears to contribute to pellicle formation in addition to saliva.
Descritores: Saliva/química
Proteínas/análise
Líquido do Sulco Gengival/química
Película Dentária/química
-Espectrometria de Massas
Western Blotting
Eletroforese em Gel de Poliacrilamida
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1019970
Autor: Borges, Cristine D Almeida; Ricoldi, Milla Sprone; Messora, Michel Reis; Palioto, Daniela Bazan; Souza, Sérgio Luís Scombatti de; Novaes Júnior, Arthur Belém; Taba Jr, Mario.
Título: Clinical attachment loss and molecular profile of inflamed sites before treatment
Fonte: J. appl. oral sci;27:e20180671, 2019. tab, graf.
Idioma: en.
Projeto: State of São Paulo Foundation (FAPESP); . Coordenação de Nível Superior - Brasil (CAPES).
Resumo: Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.
Descritores: Perda da Inserção Periodontal/patologia
-Periodontite/terapia
Saliva/química
Fatores de Tempo
Biópsia
Biomarcadores/análise
Estudos de Casos e Controles
Citocinas/análise
Líquido do Sulco Gengival/química
Estatísticas não Paramétricas
Metaloproteinase 8 da Matriz/análise
Fator A de Crescimento do Endotélio Vascular/análise
Osteoprotegerina/análise
Reação em Cadeia da Polimerase em Tempo Real
Gengiva/patologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Id: lil-549724
Autor: Rai, Balwant; Kaur, Jasdeep.
Título: Gingival crevicular fluid myeloperoxidase in periodontitis and pancreatic cancer / Níveis de mieloperoxidase no fluido crevicular gengival na periodontite e no câncer de pâncreas
Fonte: Pesqui. bras. odontopediatria clín. integr;10(1):79-82, jan.-abr. 2010.
Idioma: pt.
Resumo: Objetivo: Determinar a existência de associação entre os níveis de mieloperoxidase no fluido crevicular gengival (MFCG), periodontite e câncer de pâncreas. Método: Neste estudo duplo cego randomizado, 66 indivíduos de classe média foram divididos nos quatro grupos a seguir e tiveram os níveis de MFCG analisados: câncer de pâncreas com periodontite, câncer de pâncreas sem periodontite, periodontite sem câncer de pâncreas, e normal (33:33 mulheres:homens; faixa etária: 30-65 aos). A periodontite foi definida pela presença de pelo menos 7 dentes com profundidade de sondagem (PS) maior que 5 mm e perda óssea radiográfica perceptível maior que 30% nos sítios dentários, determinada por meio de uma série de radiografias intra-orais de boca completa. Medidas clínicas da severidade da doença periodontal, tais como sangramento à sondagem (SS), PS e perda do nível de inserção clínica (NIC), foram determinadas usando uma sonda periodontal convencional. Resultados: Os níveis médios de MFCG foram significantemente maiores nos pacientes com câncer de pâncreas e periodontite, em comparação aos outros pacientes. Os níveis médios de MFCG em pacientes sem câncer de pâncreas e pacientes com câncer de pâncreas e sem periodontite foram 0,68 ± 0,32 U/mL and 0,84±0,32 U/mL, respectivamente. Houve correlação positive entre os níveis de MFCG e as percentagens de SS, NIC e PS. Pacientes com câncer de pâncreas e periodontite apresentaram maiores percentagens de SS, PS e NIC em comparação aos pacientes sem câncer de pâncreas (p<0,05). Conclusão: A análise dos níveis de MFCG pode ser útil na detecção do risco de câncer de pâncreas em pacientes com periodontite.

Objective: To determine the association between levels of GCF myeloper oxidase (GM), periodontitis and pancreatic cancer. Method: This was a double blind randomized study. Sixty six subjects middle class divided into four group such as pancreatic cancer with periodontitis, pancreatic cancer without periodontitis, non pancreatic cancer with periodontitis and normal (33:33 M:F; range 30-65 years) were selected for the study were recruited for the study and GM levels were analyzed. Periodontitis in patients was defined of the presence of at least seven teeth with probing depth > 5mm and demonstrable radiographic bone loss >30 percent of tooth sites by a full mouth intraoal radiographic series. Clinical measures of the severity of periodontal disease, such as bleeding on probing, probing depth (PD) and loss of clinical attachment level (CL) were determined using a conventional periodontal probe. Results: GM levels were significantly higher in pancreatic cancer with periodontitis as compared to others. The mean GM level in non pancreatic cancer and pancreatic cancer without periodontitis were 0.68ñ0.32U/ml and 0.84ñ0.32 U/ ml, respectively. A positive correlation was noted between GCF meyloperoxidase and percentage of BP, CL and PD. Pancreatic cancer with periodontits, pancreatic exhibited greater BP, PD and CL as compared to non- pancreatic cancer (p<0.05). Conclusion: Thus, measurement of GM may prove to be useful in detection risk of pancreatic cancer in periodontitis patients.
Descritores: Líquido do Sulco Gengival
Neoplasias Pancreáticas
Periodontite
Peroxidase
-Método Duplo-Cego
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-990959
Autor: Arana, Paul; Salazar, Diana; Amaya, Sandra; Medina, Michelle; Moreno-Correa, Sandra; Moreno, Freddy; González, Herman; Contreras, Adolfo.
Título: Microorganismos periodontales en el líquido sinovial de pacientes con artritis reumatoide. Revisión sistemática de la literatura 2017 / Periodontal microorganisms in synovial fluid of patients with rheumatoid arthritis. Systematic review of the literature - 2017
Fonte: Rev. colomb. reumatol;25(4):271-286, oct.-dic. 2018. tab, graf.
Idioma: es.
Resumo: RESUMEN Introducción: La enfermedad periodontal (EP) y la artritis reumatoide (AR) son enfermedades inflamatorias crónicas multifactoriales que tienen en común algunos factores etiopatogénicos y la destrucción de los tejidos dentoalveolares y de las articulaciones sinoviales, de tal forma que se han identificado anticuerpos contra microorganismos periodontales en el fluido crevicular, líquido sinovial y en la membrana sinovial. Objetivo: Identificar, recuperar, analizar críticamente y sintetizar la literatura disponible acerca de la prevalencia de microorganismos periodontales en el líquido sinovial de pacientes con AR. Materiales y métodos: Se realizó búsqueda sistemática en Medline, ScienceDirect, SciELO y Google Scholar a través de los descriptores en salud Rheumatoid arthritis, periodontal microorganisms y synovial fluid. Se incluyeron artículos que describieron la presencia de microorganismos periodontales aislados en líquido sinovial de pacientes diagnosticados con AR. La búsqueda se cerró en febrero de 2017 y fue realizada con metodología PRISMA. Se emplearon las fichas de lectura crítica OSTEBA para valorar la validez externa y el nivel de evidencia de cada artículo en función del rigor metodológico. Resultados: Catorce publicaciones describieron la presencia de microorganismos periodontales en líquido sinovial de pacientes con EP y AR. Seis publicaciones realizaron detección de microorganismos periodontales en muestras de líquido sinovial, identificando en todas a P. gingivalis. Conclusiones: Los estudios incluidos evidenciaron la presencia de microrganismos periodontales en el líquido sinovial en sujetos con EP y AR, asociando la prevalencia de P. gingivalis con el aumento de los niveles de anticuerpos anti-CCP, lo que podría exacerbar los procesos inflamatorios y producir reacciones autoinmunes en AR.

ABSTRACT Introduction: Periodontal disease (PD) and rheumatoid arthritis (RA) are multifactorial chronic inflammatory diseases that share similar aetiopathogenic mechanisms that lead to the destruction of both dental-alveolar tissues and synovial joints, in such way that antibodies against periodontal pathogens have been identified in the crevicular fluid and in the synovial fluid and membranes. Objective: To identify, recover, critically analyze and synthesize the available literature on the prevalence of periodontal microorganisms in synovial fluid of patients with RA. Materials and methods: A systematic search was performed in MEDLINE, ScienceDirect, SciELO and Google Scholar using the medical subject headings "Rheumatoid arthritis", "periodontal microorganisms" and "synovial fluid". Articles were included that described the presence of isolated periodontal pathogens in synovial fluid of patients diagnosed with RA. The search was closed in February 2017 and was performed using PRISMA methodology. The OSTEBA critical reading sheets were used to assess the external validity and level of evidence of each article in terms of methodological rigor. Results: A total of 14 publications were included that described the presence of periodontal pathogens in synovial fluid of patients with PD and RA. Seven publications detected periodontal pathogens in synovial fluid, with P. gingivalis being positive in all of them. Conclusions: The included studies provided evidence of the presence of periodontal microorganisms in the synovial fluid in subjects with PD and RA, associating the prevalence of P. gingivalis with increased levels of anti-cyclic citrullinated peptide (anti-CCP) antibodies, which could exacerbate inflammatory processes and produce autoimmune reactions in RA.
Descritores: Artrite Reumatoide
Líquido Sinovial
-Doenças Periodontais
Membrana Sinovial
Líquido do Sulco Gengival
Tipo de Publ: Revisão Sistemática
Responsável: CO356.9


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Id: biblio-1120593
Autor: Mastromatteo-Alberga, Patrizzia; Escalona, Laura A; Correnti, María.
Título: Cytokines and MMPs levels in gingival crevicular fluid from patients with chronic periodontitis before and after non-surgical periodontal therapy
Fonte: J. oral res. (Impresa);7(3):98-101, mar. 28, 2018. tab.
Idioma: en.
Resumo: The study of host response in periodontal disease may provide a mechanism to monitor disease progression. the purpose of the present research was to determine the levels of IL-1alfa, IL-1beta, TNF-alfa, IL-6, IL-6sR, IL-8, IL-10, MMP-3 and MMP-8 in gingival crevicular fluid (GCF), before and after non-surgical periodontal treatment (NSPT) in order to evaluate therapy response. methodology: eleven patients diagnosed with chronic periodontitis and eleven healthy subjects were selected for this study. clinical measurements, including probing depth (PD) and clinical attachment loss (CAL) were carried out in patients diagnosed with chronic periodontitis and periodontal healthy controls. the clinical indexes evaluated were: gingival index (GI) and plaque index (PI). samples of GCF were taken from one tooth per quadrant before and 45 days after NSPT. the levels of inflammatory mediators were measured by ELISA. results: the values of all clinical parameters decressed significsntly after treatment. the concentration levels of all cytokines and MMP-3 and MMP-8 in the GCF sample were higher in patients diagnosed with chronic periodontitis compared to the healthy group. all inflammatory mediators decreased after therapy, but did not reach control values; IL-6, Il-6sR, IL-10 and TNF-alfa, attained the highest reduction (70 percent -54 percent); the vales of MMP3, IL-1alfa, IL-1beta and IL-8 were reduced between 50 percent ­ 34 percent; and MMP-8 showed the lowest decrease (28 percent). conclusion: all clinical parameters and cytokines levels decreased after NSPT. the mediators TNF-alfa IL-6, IL-6sR, and IL-10 showed the largest variation between before and after NSPT and could thus be used to evaluate therapy response.
Descritores: Líquido do Sulco Gengival/imunologia
Líquido do Sulco Gengival/microbiologia
Periodontite Crônica/metabolismo
-Biomarcadores
Estudos Prospectivos
Interleucina-8
Interleucina-6
Interleucina-1
Interleucina-10
Limites: Humanos
Adulto
Pessoa de Meia-Idade
Responsável: CL30.1 - Biblioteca


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Id: lil-746942
Autor: Obregón Miano, Fabián A; Garrido, Mauricio; Dezerega, Andrea; Hernández Ríos, Marcela.
Título: Actividad de metaloproteinasa de matriz extracelular-9 en fluido crevicular gingival durante la respuesta reparativa en periodontitis apical asintomática / Activity of matrix metalloproteinase-9 in gingival crevicular fluid during the reparative phase in asymptomatic apical periodontitis
Fonte: Rev. clín. periodoncia implantol. rehabil. oral (Impr.);8(1):54-59, 2015. ilus.
Idioma: es.
Projeto: FONDECYT.
Resumo: OBJETIVO: Los procesos destructivos del periodonto apical están en su mayoría mediados por proteasas específicas. Existe evidencia de que los niveles de metaloproteinasa de matriz extracelular-9 (MMP-9) en el fluido crevicular gingival (FCG) podrían reflejar la presencia de periodontitis apical asintomática (PAA). El objetivo de este estudio fue evaluar la actividad de MMP-9 durante la respuesta reparativa periapical en controles postendodóncicos entre una semana y 6 meses y en controles sanos. MATERIALES Y MÉTODOS: En este estudio prospectivo se incluyeron 28 pacientes con periodontitis apical asintomática, y se tomaron muestras de FCG a partir de dientes con PAA en línea base y controles postendodóncicos de una semana, uno, 3 y 6 meses. Adicionalmente se incluyeron controles contralaterales sanos. Las muestras se eluyeron y analizaron mediante gelatinogramas y densitometría. RESULTADOS: En pacientes con periodontitis apical asintomática se identificaron tanto la proforma como la forma activa de la MMP-9. Estas presentaron aumentos significativos en controles postendodóncicos de 3 y 6 meses con signos de reparación periapical. CONCLUSIONES: Los niveles de MMP-9 aumentaron significativamente en dientes con diagnóstico de PAA durante la fase reparativa. Estos resultados sugieren que MMP-9 también cumpliría un papel fisiológico durante la reparación periapical, que es susceptible de ser evaluado a través del análisis del FCG.

OBJECTIVE: Apical destructive periodontal processes are largely mediated by specific proteases. Evidence supports that the levels of extracellular matrix metalloproteinase-9 (MMP-9) could reflect the presence of asymptomatic apical periodontitis (AAP) in gingival crevicular fluid (GCF). The aim of this study was to evaluate the activity of MMP-9 during reparative response in periapical post endodontic controls between 1 week and 6 months later and in healthy controls. MATERIALS AND METHODS: A prospective study was performed on 28 patients with a diagnosis of AAP. GCF samples were taken from AAP teeth at baseline and post-endodontic controls at 1 week, 1, 3 and 6 months. Additional healthy contralateral controls were obtained, and samples were eluted and analyzed by densitometric scanning and gelatin zymography. RESULTS: In patients with asymptomatic apical periodontitis, both the pro form and the active form of MMP-9 were identified. These showed significant increases in post-endodontic controls at three and six months, with signs of periapical repairing. CONCLUSIONS: MMP-9 levels significantly increased in teeth diagnosed with AAP during the reparative phase. These results suggest that MMP-9 might be involved in the healing of apical tissues that might be reflected in GCF.
Descritores: Periodontite Periapical/enzimologia
Periodontite Periapical/terapia
Líquido do Sulco Gengival/enzimologia
Metaloproteinase 9 da Matriz/metabolismo
Limites: Humanos
Masculino
Adolescente
Adulto
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-839531
Autor: AMBRÓSIO, Lucas Macedo Batitucci; ROVAI, Emanuel da Silva; FRANÇA, Bruno Nunes de; BALZARINI, Danilo Andrés; ABREU, Ieda Santos; LOPES, Sheyla Batista Bologna; NUNES, Thaís Borguezan; LOURENÇO, Silvia Vanessa; PASOTO, Sandra Gofinet; SARAIVA, Luciana; HOLZHAUSEN, Marinella.
Título: Effects of periodontal treatment on primary sjogren's syndrome symptoms
Fonte: Braz. oral res. (Online);31:e8, 2017. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation; . São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: Abstract The aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (ESSDAI) and subjective (ESSPRI) indexes in patients with primary Sjögren’s Syndrome (pSS). Twenty-eight female patients were divided into four groups: pSS patients with or without chronic periodontitis (SCP, SC, respectively), and systemically healthy patients with or without chronic periodontitis (CP, C, respectively). Periodontal clinical examination and immunological and microbiological sample collection were performed at baseline, 30 and 90 days after nonsurgical periodontal treatment (NSPT). Levels of interleukin IL-1β, IL-8 and IL-10 in saliva and gingival crevicular fluid (GCF) were evaluated by ELISA, as well as the expression of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans, (Aa) Tannerella forsythia (Tf), and Treponema denticola (Td), by qPCR. Systemic activity and pSS symptoms were evaluated by ESSDAI and ESSPRI. NSPT resulted in improved periodontal clinical parameters in both SCP and CP groups (p>0.05). Pg, Aa, and Tf levels decreased after NSPT only in CP patients (p<0.05). Significantly greater levels of IL-10 in GCF were verified in both SCP and CP groups (p<0.05). SCP patients showed increased salivary flow rates and decreased ESSPRI scores after NSPT. In conclusion, NSPT in pSS patients resulted in improved clinical and immunological parameters, with no significant effects on microbiological status. pSS patients also showed increased salivary flow and lower ESSPRI scores after therapy. Therefore, it can be suggested that NSPT may improve the quality of life of pSS patients.
Descritores: Síndrome de Sjogren/complicações
Periodontite Crônica/etiologia
Periodontite Crônica/terapia
-Saliva/química
Salivação/fisiologia
Taxa Secretória
Fatores de Tempo
Ensaio de Imunoadsorção Enzimática
Síndrome de Sjogren/fisiopatologia
Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Estudos Prospectivos
Estudos Longitudinais
Líquido do Sulco Gengival
Interleucinas/análise
Resultado do Tratamento
Periodontite Crônica/fisiopatologia
Periodontite Crônica/microbiologia
Carga Bacteriana
Limites: Humanos
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-839530
Autor: FUKUSHIMA, Henrique; ALVES, Vanessa Tubero Euzebio; CARVALHO, Verônica Franco de; AMBRÓSIO, Lucas Macedo Batitucci; EICHLER, Rosangela Aparecida dos Santos; CARVALHO, Maria Helena Catelli de; SARAIVA, Luciana; HOLZHAUSEN, Marinella.
Título: PAR-2 expression in the gingival crevicular fluid reflects chronic periodontitis severity
Fonte: Braz. oral res. (Online);31:e16, 2017. tab, graf.
Idioma: en.
Projeto: Sao Paulo State Research Foundation.
Resumo: Abstract Recent studies investigating protease-activated receptor type 2 (PAR-2) suggest an association between the receptor and periodontal inflammation. It is known that gingipain, a bacterial protease secreted by the important periodontopathogen Porphyromonas gingivalis can activate PAR-2. Previous studies by our group found that PAR-2 is overexpressed in the gingival crevicular fluid (GCF) of patients with moderate chronic periodontitis (MP). The present study aimed at evaluating whether PAR-2 expression is associated with chronic periodontitis severity. GCF samples and clinical parameters, including plaque and bleeding on probing indices, probing pocket depth and clinical attachment level, were collected from the control group (n = 19) at baseline, and from MP patients (n = 19) and severe chronic periodontitis (SP) (n = 19) patients before and 6 weeks after periodontal non-surgical treatment. PAR-2 and gingipain messenger RNA (mRNA) in the GCF of 4 periodontal sites per patient were evaluated by Reverse Transcription Polymerase Chain Reaction (RT-qPCR). PAR-2 and gingipain expressions were greater in periodontitis patients than in control group patients. In addition, the SP group presented increased PAR-2 and gingipain mRNA levels, compared with the MP group. Furthermore, periodontal treatment significantly reduced (p <0.05) PAR-2 expression in patients with periodontitis. In conclusion, PAR-2 is associated with chronic periodontitis severity and with gingipain levels in the periodontal pocket, thus suggesting that PAR-2 expression in the GCF reflects the severity of destruction during periodontal infection.
Descritores: Líquido do Sulco Gengival/química
Receptor PAR-2/análise
Periodontite Crônica/patologia
-Valores de Referência
Índice de Gravidade de Doença
Cisteína Endopeptidases/análise
Biomarcadores/análise
Estudos de Casos e Controles
Expressão Gênica
Índice Periodontal
Índice de Placa Dentária
Perda da Inserção Periodontal
Porphyromonas gingivalis
Estatísticas não Paramétricas
Adesinas Bacterianas/análise
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-1009634
Autor: Souza, Fernando Isquierdo de.
Título: Laminados cerâmicos cimentados sobre dentes não preparados: estudo clínico, prospectivo e longitudinal sobre a adaptação marginal e avaliação do comportamento periodontal pelo uso de biomarcadores do fluido gengival crevicular / Ceramic veneers cemented on no prep teeth: prospective and longitudinal clinical study of marginal adaptation and evaluation of periodontal behavior using biomarkers of gingival crevicular fluid.
Fonte: Araçatuba; s.n; 2018. 90 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade Estadual Paulista "Julio de Mesquita Filho". Faculdade de Odontologia de Araçatuba para obtenção do grau de Doutor.
Resumo: Objetivo: Quantificar os biomarcadores e o volume do fluido gengival crevicular (GCF), com adicional verificação da adaptação e comportamento marginal de laminados cerâmicos delgados cimentados sobre dentes sem término cervical e apresentando borda marginal posicionada no interior do sulco gengival. Materiais e Métodos: 73 dentes maxilares foram tratados com laminados cerâmicos em dissilicato de lítio monolítico. Para todos os elementos foi feita a coleta do GCF previamente à cimentação (baseline) e nos tempos do estudo (t0- 7 dias, t4 180 dias), onde foi calculado o volume do GCF e quantificado os biomarcadores: TNF-α, IL-1ß, MMP-8 por ELISA. O dente vizinho ou antagonista foi utilizado como controle. Foram obtidas réplicas da região cervical em resina epóxi, em t0 e t4 para verificação da adaptação marginal em microscópio eletrônico de varredura. A avaliação clínica foi realizada pelos critérios modificados do USPHS nos tempos do estudo e com 1 ano de acompanhamento (t5). Resultados: Os níveis de TNF-α e MMP-8 se mantiveram estáveis na comparação de t4 com o baseline (p>0,05). A concentração de IL-1ß aumentou em t4 no grupo tratado em comparação com o baseline (p>0,05) porém sem diferença estatística significante para o grupo controle em t4 e no baseline (p>0,05). Todos os elementos obtiveram conceito alfa para avaliação clínica e microscópica. Conclusão: No período de acompanhamento proposto pelo presente estudo, o periodonto de proteção apresentou excelente comportamento clínico e biológico frente ao tratamento com laminados cerâmicos sem preparo cervical, não apresentando diferenças morfológicas e bioquímicas em comparação aos dentes não tratados(AU)

Objective: To quantify the biomarkers and gingival crevicular fluid (GCF) volume, with additional verification of the adaptation and marginal behavior of thin ceramic veneers cemented on teeth without cervical preparation, with the finish line gently positioned within the gingival sulcus. Materials and Methods: 73 maxillary teeth were treated with monolithic lithium disilicate ceramic veneers. For all elements, the GCF was collected before cementation (baseline) and at the study times (t0 -7 days, t4 180 days), which was calculated the GCF volume and the quantification of the biomarkers: TNF-α, IL- 1ß, MMP-8 by ELISA. The neighboring or antagonist tooth was used as a control. Replicas of the cervical region were obtained in epoxy resin, in t0 and t4 to verify the marginal adaptation in a scanning electron microscope. Clinical evaluation was performed using the modified USPHS criteria at study times and at 1 year of follow-up (t5). Results: TNF-α and MMP-8 levels remained stable in the comparison of t4 with the baseline (p>0.05). The concentration of IL-1ß increased in t4 in the treated group compared to the baseline (p>0.05), but without significant statistical difference for the control group in t4 and baseline (p>0.05). All the elements obtained alpha concept for clinical and microscopic evaluation. Conclusion: In the period of follow-up proposed by the present study, the protection periodontium presented excellent clinical and biological behavior in relation to the treatment with ceramic veneers without cervical preparation, presenting no morphological and biochemical differences in comparison to untreated teeth(AU)
Descritores: Cerâmica
Líquido do Sulco Gengival
Adaptação Marginal Dentária
Facetas Dentárias
-Estética Dentária
Limites: Humanos
Masculino
Feminino
Responsável: BR186.1 - Biblioteca Honório Monteiro
BR186.1



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