Base de dados : LILACS
Pesquisa : A13.080 [Categoria DeCS]
Referências encontradas : 16 [refinar]
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Id: biblio-1278436
Autor: Martins, Thais Serra; Sousa, Thiago Sousa e; Sales, Victor Hugo Gomes; Bandeira, Maria da Gloria Almeida; Higuita, Diana Maria Cano; Vélez, Harvey Alexander Villa.
Título: Comparison between Thin-Layer Models and Non-Traditional Methods in the Modelling of Drying Kinetics of Crustacean Wastes
Fonte: Braz. arch. biol. technol;64:e21210130, 2021. tab, graf.
Idioma: en.
Projeto: Maranhão Foundation for the Protection of Research and Scientific and Technological Development.
Resumo: Abstract This research aims to compare the classical thin-layer models, stepwise fit regression method (SRG) and artificial neural networks (ANN) in the modelling of drying kinetics of shrimp shell and crab exoskeleton. Thus, drying curves were obtained using a convective dryer (3.0 m/s) at temperatures of 30.45 and 60oC. The results showed a decreasing tendency for the drying time as the temperature increased for both materials. Drying curves modelling of both materials showed fitted results with R 2 adj >0.998 and MRE<13.128% for some thin-layer models. On the other hand, by SRG a simple model could be obtained as a function of time and temperature, with the greatest accuracy being found in the modelling of experimental data of crab exoskeleton, with MRE<10.149%. Finally, the ANNs were employed successfully in the modelling of drying kinetics, showing high prediction quality with the trained recurrent ANN models.
Descritores: Crustáceos
Exoesqueleto
-Cinética
Redes Neurais de Computação
Modelos Anatômicos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: biblio-950711
Autor: Promphet, Porkaew; Bunarsa, Sirirat; Sutheerawattananonda, Manote; Kunthalert, Duangkamol.
Título: Immune enhancement activities of silk lutein extract from Bombyx mori cocoons
Fonte: Biol. Res;47:1-10, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Declining immune function poses an important clinical challenge worldwide and supplementation with natural products that possessing immune enhancing properties is a promising approach for preventing or delaying immune function decline. Cocoons from yellow silkworms are a significant source of lutein, and this unexplored silk extract could be a viable alternative source for dietary lutein. This study assessed immunomodulatory activities of the silk lutein extract. Female BALB/c mice orally received lutein, either as silk or marigold extracts (10 or 20 mg/kg daily), or vehicle only (1% tween 80 in PBS pH 7.4) for 4 weeks. Natural killer (NK) cell activity, specific antibody production, lymphocyte subpopulations, mitogen-induced lymphocyte proliferation, and cytokine production were examined. RESULTS: Silk lutein extract increased NK cell activity, and the effect was dose-related whereas marigold lutein extract was ineffective. Silk lutein extract dose-dependently enhanced antibody production in pre-immunized mice but marigold lutein extract had no effect. Feeding with silk lutein extract increased the populations of CD3+ and CD4 + CD3 + cells. Silk lutein extract also stimulated concanavalin A- and lipopolysaccharide-induced proliferations of T and B lymphocytes, respectively. Moreover, silk lutein extract increased IL-2 and IFN-γ production while the effect of marigold lutein extract was undetectable. CONCLUSIONS: Together, silk lutein extract enhanced both innate and adaptive immune functions. This preparation may prove to be an effective supplement for strengthened immunity.
Descritores: Bombyx/imunologia
Extratos de Tecidos/imunologia
Luteína/imunologia
Seda/imunologia
Exoesqueleto/química
Fatores Imunológicos/análise
-Pupa/imunologia
Pupa/metabolismo
Bombyx/metabolismo
Extratos de Tecidos/farmacologia
Luteína/isolamento & purificação
Anticorpos Heterófilos/sangue
Extratos Vegetais/imunologia
Linfócitos B/efeitos dos fármacos
Células Matadoras Naturais/efeitos dos fármacos
Células Matadoras Naturais/imunologia
Linfócitos T/efeitos dos fármacos
Interleucina-4/análise
Interferon gama/análise
Interleucina-2/análise
Interleucina-10/análise
Tagetes/imunologia
Flores/imunologia
Seda/química
Proliferação de Células/efeitos dos fármacos
Citometria de Fluxo
Camundongos Endogâmicos BALB C
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: biblio-950713
Autor: Yan, Yizhong; Li, Jianjun; Zhang, Yiya; Peng, Xiaozhen; Guo, Tianyao; Wang, Jirong; Hu, Weijun; Duan, Zhigui; Wang, Xianchun.
Título: Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
Fonte: Biol. Res;47:1-11, 2014. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Hunan Provincial Natural Science Foundation of China; . Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province. National Basic Research Program or \"973 Program.
Resumo: BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions.
Descritores: Óvulo/química
Extratos de Tecidos/química
Viúva Negra/química
Proteínas de Artrópodes/toxicidade
-Óvulo/fisiologia
Nervo Frênico/efeitos dos fármacos
Extratos de Tecidos/toxicidade
Canais de Cálcio/efeitos dos fármacos
Baratas/efeitos dos fármacos
Canais de Potássio de Abertura Dependente da Tensão da Membrana/efeitos dos fármacos
Exoesqueleto/fisiologia
Exoesqueleto/química
Proteínas de Artrópodes/isolamento & purificação
Canais de Sódio Disparados por Voltagem/efeitos dos fármacos
Gânglios Espinais/efeitos dos fármacos
Limites: Animais
Camundongos
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Costa Rica
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Id: biblio-1003330
Autor: Donato-Rondón, Jhon C; González-Trujillo, Juan David; Romero, Buendy; Castro-Rebolledo, Maria I.
Título: Diatom assemblages associated with turtle carapaces in the Neotropical region / Diatomeas epizoicas de los caparazones de tortugas de la región Neotropical
Fonte: Rev. biol. trop;66(4):1362-1372, oct.-dic. 2018. graf.
Idioma: en.
Resumo: Abstract Few studies have explored the ecology and interrelationship with other organisms of the many endangered freshwater turtle species inhabiting the Neotropical region. The focus of the current study was to shed light on the relationship between Neotropical turtle carapaces and primary producers, insofar as the surface of former constitutes a suitable substrate for the colonization and establishment of the latter. The under-explored relationship between turtle carapaces and the diatom assemblages inhabiting them was investigated and characterized in terms of taxonomic and biological traits (bio-volume, life-form, and attachment). The carapaces of seven native turtle species were surveyed. Among these carapaces, a total of 45 diatom taxa were found, and diatom taxa varied among turtle species. Podocnemis vogli and Podocnemis expansa supported more diverse diatom assemblages than Podocnemis lewyana, Rhinoclemmys diademata, and Rhinoclemmys melanosterna, which were dominated by Navicula spp. Analysis, further showed that carapace size did not explain differences in diatom diversity. However, a trait-based analysis suggests that both carapace roughness and an assemblage's successional stage might explain the differences in assemblage composition. Because turtles can serve as dispersal vectors, characterizing their epibiont diatom assemblages may contribute to our understanding of diatom distribution on larger scales, as well as, give us some clues as to the auto-ecology of turtles that help us to effectively determine conservation areas for these endangered species.(AU)

Resumen De las especies de tortugas en estado crítico o vulnerable de conservación que habitan en la región Neotropical, son pocos los estudios que han ahondado en su ecología o relaciones con otros tipos de organismos. El presente estudio buscó caracterizar la asociación existente entre las diatomeas y el caparazón de especies de tortugas neotropicales, ya que este representa un sustrato adecuado para la colonización y establecimiento de este grupo de algas. Esta asociación fue estudiada considerando tanto la composición de especies como la de rasgos biológicos (biovolumen, forma de vida, y forma de adherencia) de la comunidad de diatomeas. En los caparazones de las 7 especies de tortugas muestreadas se encontraron un total de 45 táxones. La composición de táxones varió entre los caparazones de las especies. Se encontró una mayor diversidad en los caparazones de Podocnemis vogli y Podocnemis expansa, mientras que en Podocnemis lewyana, Rhinoclemmys diademata, y Rhinoclemmys melanosterna se encontró una comunidad de baja diversidad dominada principalmente por Navicula sp. Los resultados sugirieron que la rugosidad del caparazón junto al estado de la sucesión pueden explicar las diferencias observadas en la composición de táxones de los diferentes caparazones. Debido a que las tortugas pueden funcionar como vectores de dispersión, caracterizar las comunidades que pueden crecer sobre sus caparazones podría contribuir a entender la distribución de las diatomeas a escalas mayores. Además, esta caracterización podría darnos pistas clave para la delimitación de áreas de conservación efectivas para las especies más amenazadas.(AU)
Descritores: Tartarugas
Diatomáceas
Exoesqueleto
-Colômbia
Responsável: BR1.1 - BIREME


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Id: biblio-886265
Autor: Andrade, Paulo Henrique Muleta; Portugal, Luciane Canderolo; Rondon, Eric Schmidt; Kadri, Monica Cristina Toffoli; Matos, Maria de Fátima Cepa.
Título: Effect of powdered shells treatment of the snail Megalobulimus lopesi on wounds of diabetic rats
Fonte: Acta cir. bras;33(2):185-196, Feb. 2018. graf.
Idioma: en.
Resumo: Abstract Purpose: To analyzed the healing effect of the powdered shell of the Megalobulimus lopesi snail on wounds of diabetic rats, since in non-diabetic rats the powdered shell presented healing potential. Methods: Seventy-two Wistar rats (Rattus norvegicus albinus) were divided into three groups: Control group (GC.diab), no therapeutic intervention on the wound; Vehicle's Control group, topical via, in diabetic rats (GCvt.diab): Powder Shell Group (PC) applied topically (GPCvt.diab): Experimental group was administered topically shortly after wound dressing and once a day during the experimental period (3, 7, 14 and 21 days) the composition containing the powdered shell of the snail. The following variables related to the healing potential were analyzed: macroscopic one, where the capacity of reduction of the wound area was evaluated; histological analysis in HE, angiogenic activity, morphometric analysis (re-epithelization), leukocyte inflammatory infiltrate; leukocyte count and also differentiation in peripheral blood. Results: The topical application in wounds of diabetic rats presented healing activity, accelerating wound closure, stimulating angiogenesis and being pro-inflammatory in the early and anti-inflammatory stages in the final times of the healing process. Conclusion: The topical administration of the powdered shell on wounds of diabetic patients becomes a therapeutic option of low cost, with ease in the administration and access as well.
Descritores: Caramujos
Extratos de Tecidos/farmacologia
Cicatrização/efeitos dos fármacos
Diabetes Mellitus Experimental/fisiopatologia
Exoesqueleto/química
Anti-Inflamatórios/farmacologia
-Pós
Extratos de Tecidos/administração & dosagem
Administração Tópica
Ratos Wistar
Modelos Animais de Doenças
Reepitelização
Anti-Inflamatórios/administração & dosagem
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-893154
Autor: Makalani, Fatemeh; Khazaei, Mohammad Rasool; Ghanbari, Elham; Khazaei, Mozafar.
Título: Crab shell extract improves serum biochemical markers and histological changes of pancreas in diabetic rats / El extracto de cáscara de cangrejo mejora los marcadores bioquímicos del suero y los cambios histológicos del páncreas en ratas diabética
Fonte: Int. j. morphol;35(4):1437-1443, Dec. 2017. tab, graf.
Idioma: en.
Resumo: SUMMARY: Diabetes mellitus is a common metabolic disease. There are many natural agents available to control and treat diabetes. Crab shell extract has antioxidant properties. The aim of present study was to investigate the effect of crab shell hydroalcoholic extract on blood glucose, liver enzymes, nitric oxide and antioxidant capacity of serum and histological structure of pancreas in diabetic rats. In this experimental study, thirty five male Wistar rats (180-220 g) were divided into control, diabetic and experimental groups (n=7). Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Rats were treated for 14 days by crab shell extract with 100, 200 and 400 mg/kg doses. Fasting blood glucose, serum levels of liver enzymes, nitric oxide (NO) and total antioxidant capacity were evaluated. Changes of pancreatic tissue were determined using a modified aldehyde fuchsin staining method. Data were analyzed using one-way ANOVA. Differences were considered statistically significant at P<0.05. Crab shell extract induced a significant reduction in blood glucose, serum levels of nitric oxide and ALT (P=0.033). Also, there were a significant increase in total antioxidant capacity (FRAP) (P=0.007), and insignificant decrease in serum levels of AST. The extract improved pancreatic tissue changes caused by diabetes. In conclusion, antioxidant and anti-diabetic effects of crab shell increase total antioxidant capacity of serum and decreased blood glucose, serum nitric oxide and ALT levels.

RESUMEN: La diabetes mellitus es una enfermedad metabólica común. Hay muchos agentes naturales disponibles para controlar y tratar la diabetes. El extracto de cáscara de cangrejo tiene propiedades antioxidantes. El objetivo del presente estudio fue investigar el efecto del extracto hidroalcohólico de la cáscara de cangrejo sobre la glucosa sérica, las enzimas hepáticas, el óxido nítrico y la capacidad antioxidante del suero y la estructura histológica del páncreas en ratas diabéticas. En este estudio experimental, treinta y cinco ratas Wistar machos (180220 g) se dividieron en cinco grupos: control, diabéticos y experimentales (n = 7). La diabetes se indujo por inyección intraperitoneal de estreptozotocina (60 mg / kg). Las ratas se trataron durante 14 días con extracto de cáscara de cangrejo con dosis de 100, 200 y 400 mg / kg. Se evaluaron la glucosa en sangre en ayunas, las enzimas hepáticas, el óxido nítrico sérico y la capacidad antioxidante total. Los cambios en el tejido pancreático se determinaron usando un método de tinción de aldehído fucsina modificado. Los datos se analizaron utilizando ANOVA unidireccional. Las diferencias se consideraron estadísticamente significativas a P <0,05. El extracto de cáscara de cangrejo indujo una reducción significativa en la glucosa en sangre, en los niveles séricos de óxido nítrico y ALT (P = 0,033). Además se observó un aumento significativo en la capacidad antioxidante total (FRAP) (P = 0.007), y una disminución insignificante en los niveles séricos de AST. El extracto mejoró los cambios en el tejido pancreático causados por la diabetes. En conclusión, los efectos antioxidantes y antidiabéticos de la cáscara de cangrejo aumentan la capacidad antioxidante total de suero y la disminución de la glucosa en la sangre, el óxido nítrico sérico y los niveles de ALT.
Descritores: Exoesqueleto/química
Antioxidantes/administração & dosagem
Misturas Complexas/administração & dosagem
Diabetes Mellitus Experimental/tratamento farmacológico
Pâncreas/efeitos dos fármacos
-Glicemia/efeitos dos fármacos
Braquiúros
Óxido Nítrico/sangue
Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Silva, José Carlos
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Id: biblio-868060
Autor: Fransozo, Adilson; Teixeira, Gustavo Monteiro; Gomes, Rafael de Rocco; Silva, José Carlos; Bolla Junior, Eduardo Antonio.
Título: Ocorrência de anormalidades morfológicas externas em caranguejos marinhos (Decapoda, Brachyura) no litoral norte do Estado de São Paulo / Occurrence of external morphological abnormalities in marine crabs (Decapoda, Brachyura) from north coast of the São Paulo State
Fonte: Acta sci., Biol. sci;34(1):101-104, Jan.-Mar. 2012. ilus.
Idioma: en.
Resumo: Deformidades e anormalidades em crustáceos têm sido associadas a fatores genéticos, problemas ocorridos durante a muda, danos causados por ectobiontes, predadores ou estresse ambiental causado por produtos químicos. Espécimes de caranguejos coletados no litoral de São Paulo apresentaram anormalidades no corpo. Estes caranguejos pertencem às seguintes espécies: Callinectes ornatus (Ordway, 1863), Arenaeus cribrarius (Lamarck, 1818) e Leurocyclus tuberculosus (H. Milne Edwards & Lucas, 1843). As coletas foram realizadas por meio de arrastos camaroneiros em julho de 2008, agosto e outubro de 2009, na região de Ubatuba, Estado de São Paulo, Brasil. As anormalidades apresentadas no corpo dos caranguejos encontravam-se no dáctilo do quelípodo (C. ornatus macho adulto), defeitos na carapaça (A. cribrarius macho adulto) e alterações abdominais (C. ornatus fêmea adulta; L. tuberculosus macho adulto e fêmea ovígera). O registro de tais ocorrências pode servir na distinção de alterações causadas naturalmente ou por impacto humano, podendo fornecer ferramentas úteis no sentido de monitorar áreas ambientais não protegidas, bem como trazer subsídios ao entendimento de alterações não usuais ocorridas durante a ontogenia de espécies importantes para a comunidade bentônica.

Deformities and abnormalities in crustaceans have been associated to genetic problem, which occurred during molt process, damage caused by ectobionts, predators or environmental stress caused by chemical wastes. Some crab specimens collected in the São Paulo littoral were found having body abnormalities. They belong to the following crab species: Callinectes ornatus (Ordway, 1863), Arenaeus cribrarius (Lamarck, 1818) and Leurocyclus tuberculosus (H. Milne Edwards; Lucas, 1843). Samplings were performed by trawling during July 2008, August and October 2009 at the Ubatuba region, São Paulo State, Brazil. Body abnormalities were verified in the cheliped dactyl (C. ornatus an adult male), carapace deformities (A. cribrarius an adult male) and abdominal alterations (C. ornatus an adult female; L. tuberculosus an adult male and an ovigerous female). The record and analysis of such occurrences can help in the distinction of natural or human impact caused alterations. In this way, the occurrence study of this kind of body alterations could provide tools in order to control unprotected environmental areas, as well as bring subsides to understand the unusual variations during the ontogeny of important species in the benthic community.
Descritores: Exoesqueleto
Anormalidades Congênitas
Xiphosura americana
Responsável: BR513.2 - Editora da Universidade Estadual de Maringá


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Texto completo SciELO Brasil
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Id: lil-787585
Autor: Vieira, Lucélia G; Santos, André L. Q; Moura, Léa R; Orpinelli, Stiwens R. T; Pereira, Kleber F; Lima, Fabiano C.
Título: Morphology, development and heterochrony of the carapace of giant amazon river turtle podocnemis expansa (testudines, podocnemidae) / Morfologia, desenvolvimento e heterocronia da carapaça da tartagura da amazônia, podocnemis expansa (testudines, podocnemidae)
Fonte: Pesqui. vet. bras = Braz. j. vet. res;36(5):436-446graf.
Idioma: en.
Resumo: With aim to report the ontogeny of the osseous elements of the carapace in Peurodiras, 62 embryos and 43 nestlings of Podocnemis expansa were collected and submitted to the clearing and staining technique of bones and cartilages and study of serial histological slices. The carapace has mixed osseous structure of endo and exoskeleton, formed by 8 pairs of costal bones associated with ribs, 7 neural bones associated with neural arches, 11 pairs of peripheral bones, 1 nuchal, 1 pygal and 1 suprapygal. This structure begins its formation in the beginning of stage 16 with the ossification of the periosteal collar of the ribs. With exception of the peripheral bones, the other ones begin their ossification during the embrionary period. In histologic investigation it was found that the costal bones and neural bones have a close relation to the endoskeleton components, originating themselves as intramembranous expansions of the periosteal collar of the ribs and neural arches, respectively. The condensation of the mesenchyme adjacent to the periosteal collar induces the formation of spikes that grow in trabeculae permeated by fibroblasts below the dermis. The nuchal bone also ossifies in an intramembranous way, but does not show direct relation to the endoskeleton. Such information confirms those related to the other Pleurodira, mainly with Podocnemis unifilis, sometimes with conspicuous variations in the chronology of the ossification events. The formation of dermal plates in the carapace of Pleurodira and Criptodira follow the same pattern.

Com objetivo de relatar a ontogenia dos elementos ósseos da carapaça em Pleurodiras, coletaram-se 62 embriões e 43 filhotes de Podocnemis expansa que foram submetidos à técnica de clareamento e coloração dos ossos e cartilagens e pelo estudo cortes histológicos seriados. A carapaça possui estrutura óssea mista de endo e exoesqueleto, sendo formada por 8 pares de ossos costais associados às costelas, 7 ossos neurais associados aos arcos vertebrais, 11 pares de ossos periféricos, 1 nucal, 1 pigal e 1 supra-pigal. Esta estrutura começa sua formação no início do estágio 16 com a ossificação do colar periostal das costelas. Com exceção dos ossos periféricos, os demais iniciam sua ossificação durante o período embrionário. A investigação histológica explicitou que os ossos costais e neurais possuem uma estreita relação com componentes do endoesqueleto, originando-se como expansões intramembranosas do colar periostal das costelas e dos arcos neurais, respectivamente. A condensação do mesenquima adjacente ao colar periostal induz a formação de espiculas que crescem em trabéculas permeadas por fibroblastos abaixo da derme. O osso nucal, também se ossifica de maneira intramembranosa mas não apresenta relação direta com o endoesqueleto. Tais informações corroboram àquelas relatadas para os demais Pleurodiras, principalmente com Podocnemis unifilis, outrora com variações conspícuas na cronologia dos eventos de ossificação. A formação das placas dérmicas da carapaça em Pleurodira e Criptodira seguem um mesmo padrão.
Descritores: Desenvolvimento Ósseo/fisiologia
Exoesqueleto/anatomia & histologia
Tartarugas/anatomia & histologia
-Osteogênese
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Brasil
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Id: lil-779792
Autor: Pierri, B. S.; Fossari, T. D.; Magalhães, A. R. M..
Título: O mexilhão Perna perna no Brasil: nativo ou exótico? / The brown mussel Perna perna in Brazil: native or exotic?
Fonte: Arq. bras. med. vet. zootec;68(2):404-414, mar.-abr. 2016. tab, graf.
Idioma: pt.
Resumo: A mitilicultura no Brasil se baseia no mexilhão Perna perna (Linnaeus, 1758), que é encontrado em todo o litoral brasileiro, sendo especialmente abundante do Espírito Santo a Santa Catarina. Nos últimos anos, foi lançada a hipótese de que P. perna seja uma espécie exótica no litoral brasileiro. A hipótese se baseia na análise da malacofauna presente em sítios arqueológicos. Todas as contestações levantadas não remetem à uma conclusão, pois precisam de estudos específicos, com metodologias claras, aliando arqueologia, ecologia e biologia molecular. O objetivo deste trabalho foi estudar a condição de nativo ou exótico do mexilhão Perna perna no Brasil, a partir de levantamento dos resultados em sítios arqueológicos, de técnicas moleculares e de datação de conchas com C14. A datação indicou que as amostras de Perna perna do sítio arqueológico Rio do Meio/Jurerê, Florianópolis/SC, têm idade de 720±30 e 780±30 anos. O cálculo do tempo de divergência indicou que a separação das populações brasileiras e africanas ocorreu por volta de 200 mil anos. Os resultados apontam a presença da espécie no território brasileiro muito antes do descobrimento do Brasil pelos portugueses no ano de 1500, indicando que P. perna é de fato uma espécie nativa.

Mussel farming in Brazil is based on brown mussel Perna perna (Linnaeus, 1758), which is found throughout the Brazilian coast, especially abundant from Espírito Santo to Santa Catarina coast. In recent years it was suggested that Perna perna is an exotic species for the Brazilian coast. The hypothesis is based on the analysis of the zooarchaeology studies in archaeological sites in Brazil. All objections raised do not offer a conclusion, because they need specific studies with clear methodology, combining archeology, ecology and molecular biology. The aim of this work was to study the condition of the brown mussel Perna perna in Brazil if native or exotic, from survey results in archaeological sites, molecular techniques and dating of shells with C14. The dating indicated that the shells were 720±30 and 780±30 years, respectively. The calculation of divergence time indicated that the separation of the African and Brazilian mussel populations occurred around 200 thousand years ago. The results indicate the presence of the P. perna species in Brazilian territory long before the discovery of Brazil by the Portuguese in 1500, indicating that P. perna is actually a native species of Brazil.
Descritores: Aquicultura
Águas Salinas/análise
Biologia Molecular
Perna (Organismo)
-Exoesqueleto/anatomia & histologia
Variação Genética
Moluscos
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-771893
Autor: Calais Júnior, A; David, J. A. O.; Nunes, L. C..
Título: Caracterização morfológica do esôfago de tartarugas-verdes (Chelonia mydas) / Morphologic characterization of the esophagus of green turtles (Chelonia mydas)
Fonte: Arq. bras. med. vet. zootec;68(1):127-135, jan.-fev. 2016. tab, graf.
Idioma: pt.
Resumo: A tartaruga-verde, Chelonia mydas (Linnaeus, 1758), é a mais comum na região costeira do Brasil e a compreensão da morfologia e de processos fisiológicos básicos nesses organismos é importante, podendo fornecer subsídios para estudos evolutivos sobre a espécie. O objetivo deste trabalho foi descrever a morfologia do esôfago de C. mydas, com a finalidade de se produzirem informações que possibilitem análises comparativas com outros quelônios. Utilizaram-se oito animais, provenientes do litoral do Espírito Santo, no período de setembro a novembro de 2014. Obtiveram-se dados de comprimento curvilíneo da carapaça (CCC) e largura curvilínea da carapaça (LCC). O esôfago foi retirado desde a inserção da orofaringe até a porção inicial do estômago para avaliação histomorfológica e medida do comprimento. Foram avaliadas quatro regiões do esôfago. O órgão inteiro foi fixado em formalina a 10% e, posteriormente, foi coletado um fragmento de cada região para processamento histológico e coloração pelas técnicas hematoxilina e eosina (HE), tricrômico de Masson, Gram e ácido periódico-Schiff (PAS). Os animais juvenis possuíam CCC médio de 38,8±6,43cm e LCC média de 35,6±6,59cm; o animal adulto mediu 102,3cm de CCC e 96,9cm de LCC. O comprimento médio do esôfago foi de 20,47±1,56 nos juvenis, e total de 55,6cm no adulto. Observou-se que o esôfago de C. mydas é um órgão tubular muscular constituído de papilas cônicas que variam de quantidade e tamanho à medida que se aproximam do estômago, revestidas por epitélio estratificado pavimentoso queratinizado e ricas em tecido mixoide. Há variação nas camadas mucosa, muscular externa e serosa entre as regiões do esôfago e há presença de glândulas produtoras de muco na mucosa da região da junção gastroesofágica. As características histomorfológicas do esôfago de C. mydas são importantes para exercer as funções mecânica e de proteção da mucosa desse órgão.

Understanding the morphology and basic physiological processes in Chelonia mydas is extremely important and can provide support for evolutionary studies on the species. The aim of this study was to describe the morphology of C. mydas' esophagus in order to produce data that allows comparative analyses with other turtles. Eight specimens of C. mydas from the coast of Espírito Santo were used during the period from September to November 2014. We obtained the curved carapace length (CCL) and curved carapace width (CCW) in centimetres. The esophagus was completely removed from the insertion region of the oropharynx to the initial portion of the stomach for macroscopic and microscopic evaluation. The measurement on the length and description of the structures in four regions of the esophagus were performed. The entire organ was fixed in 10% formalin and then a one centimetre diameter fragment was collected for histological processing and staining by hematoxylin and eosin (HE), Masson, Gram and periodic acid-Schiff (PAS) techniques. Juvenile animals had average CCL of 38.8 ± 6.43 cm and average CCW of 35.6 ± 6.59 cm; the adult animal had a CCL measurement of 102.3 cm and CCW of 96.9 cm. The average length of the esophagus was 20.47 ± 1.56 in youth, and total 55.6 cm in the adult. It was observed that C. mydas' esophagus is a tubular muscular organ that consists of conical papillae that vary in quantity and size as they approach the stomach, lined by stratified squamous keratinized epithelium and rich in myxoid tissue. There is variation in the mucosal, submucosal, external muscle and serous layers among the four regions of the esophagus and there are mucus-producing glands in the submucosa of the gastroesophageal junction region. The histomorphometric characteristics of C. mydas' esophagus are important to engage the mechanical function and mucosal protection of this organ.
Descritores: Exoesqueleto
Esôfago
Estômago/anatomia & histologia
Trato Gastrointestinal
Tartarugas/anatomia & histologia
-Histologia
Orofaringe
Pesos e Medidas Corporais/veterinária
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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