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Id: lil-509329
Autor: Sanabe, Mariane Emi; Giorgetti, Ana Paula de Oliveira; Cruz, Alice Rodrigues da; Hebling, Josimeri.
Título: Influência da contaminação da dentina por cimentos temporários naresistência da união de sistemas adesivos / Influence of dentin contamination by temporary cements on the bond strength of adhesive systems
Fonte: RGO (Porto Alegre);57(1), jan.-mar. 2009. ilus, tab.
Idioma: pt.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo.
Resumo: Objetivo: Avaliar a resistência da união de sistemas adesivos à dentina contaminada por cimentos temporários com ou sem eugenol. Método: Foram obtidas superfícies planas de dentina de 24 terceiros molares humanos. Com exceção do grupo controle (n=8), as superfícies foram cobertas com Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) ou Cavit (3M ESPE, St. Paul, MN, EUA) e mantidas em estufa a 37°C por sete dias. Após a remoção dos cimentos, os sistemas adesivos Adper Single Bond (3M ESPE, St. Paul, MN, EUA) ou Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japão) foram aplicados segundo a recomendação dos fabricantes e, em seguida, realizadas as construções de coroas em resina composta. Os dentes foram secionados em espécimes com área transversal de união de 0,81mm², os quais foram submetidos ao teste de microtração em máquina para ensaios mecânicos com velocidade do atuador de 0,5mm/min. Os dados foram analisados por testes t e Anova, complementada por testes de Tukey (α=0,05). Resultados: Para Adper Single Bond (3M ESPE, St. Paul, MN, EUA), a resistência de união foi estatisticamente não-diferente (p>0,05) para todas as condições experimentais. Para Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japão), apenas o grupo Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) apresentou resistência de união significativamente inferior (30,1±13,8 MPa) em relação aos demais grupos; controle (38,9±13,5 MPa) e Cavit (3M ESPE, St. Paul, MN, EUA) (42,1±11,0 MPa), os quais não apresentaram diferença significativa entre si. Conclusão: Concluiu-se que o recobrimento prévio da dentina com cimento temporário, contendo eugenol, exerceu efeito deletério apenas no desempenho adesivo do sistema autocondicionante.

Objective: The aim of this study was to assess the bond strength of adhesive systems to dentin contaminated by temporary cements with or without eugenol. Method: Flat dentin surfaces were obtained from twenty-four human third molars. With exception of the control group (n=8), the surfaces were covered with Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) or Cavit (3M ESPE, St. Paul, MN, USA) and kept in an oven at 37°C for seven days. After removing the cements, the adhesive systems Adper Single Bond (3M ESPE, St. Paul, MN, USA) or Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan) were applied in accordance with the manufacturers' recommendations, and then the crowns were constructed in of resin composite. The teeth were sectioned into specimens with a cross-sectional bond area of 0.81mm², which were submitted to microtensile testing in a mechanical test machine at an actuator speed of 0.5mm/min. The data were analyzed by t- and ANOVA tests, complemented by Tukey tests (α=0.05). Results: For Adper Single Bond (3M ESPE, St. Paul, MN, USA), bond strength did not differ statistically (p>0.05) for all the experimental conditions. For Clearfil SE Bond (Kuraray Co. Ltd., Osaka, Japan), only the Interim Restorative Material (Caulk Dentsplay, Milford, DE, USA) Group showed significantly lower bond strength (30.1±13.8 MPa) in comparison with the other groups; control (38.9±13.5 MPa) and Cavit (3M ESPE, St. Paul, MN, USA) (42.1±11.0 MPa), which showed no significant difference between them. Conclusion: It was concluded that the previous covering of dentin with temporary cement containing eugenol had a deleterious effect on the adhesive performance of the self-etching system only.
Descritores: Adesivos Dentinários
Cimentos Dentários/efeitos adversos
Contaminação
Dentina
-Resistência à Tração
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR541.1 - Biblioteca


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Id: lil-797977
Autor: Lei, Lei; Shao, Meiying; Yang, Yan; Mao, Mengying; Yang, Yingming; Hu, Tao.
Título: Exopolysaccharide dispelled by calcium hydroxide with volatile vehicles related to bactericidal effect for root canal medication
Fonte: J. appl. oral sci;24(5):487-495, Sept.-Oct. 2016. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: ABSTRACT Objective: Enterococcus faecalis is the dominant microbial species responsible for persistent apical periodontitis with ability to deeply penetrate into the dentin. Exopolysaccharides (EPS) contribute to the pathogenicity and antibiotic resistance of E. faecalis. Our aim was to investigate the antimicrobial activity of calcium hydroxide (CH), camphorated parachlorophenol (CMCP), and chlorhexidine (CHX) against E. faecalis in dentinal tubules. Material and Methods: Decoronated single-canal human teeth and semicylindrical dentin blocks were incubated with E. faecalis for 3 weeks. Samples were randomly assigned to six medication groups for 1 week (n=10 per group): CH + 40% glycerin-water solution (1:1, wt/vol); CMCP; 2% CHX; CH + CMCP (1:1, wt/vol); CH + CMCP (2:3, wt/vol); and saline. Bacterial samples were collected and assayed for colony-forming units. After dentin blocks were split longitudinally, confocal laser scanning microscopy was used to assess the proportion of viable bacteria and EPS production in dentin. Results: CMCP exhibited the best antimicrobial activity, while CH was the least sensitive against E. faecalis (p<0.05). CHX showed similar antimicrobial properties to CH + CMCP (1:1, wt/vol) (p>0.05). CH combined with CMCP inhibited EPS synthesis by E. faecalis, which sensitized biofilms to antibacterial substances. Moreover, increasing concentrations of CMCP decreased EPS matrix formation, which effectively sensitized biofilms to disinfection agents. Conclusion: The EPS matrix dispelled by CH paste with CMCP may be related to its bactericidal effect; the visualization and analysis of EPS formation and microbial colonization in dentin may be a useful approach to verify medicaments for antimicrobial therapy.
Descritores: Polissacarídeos Bacterianos/química
Irrigantes do Canal Radicular/farmacologia
Veículos Farmacêuticos/farmacologia
Hidróxido de Cálcio/farmacologia
Enterococcus faecalis/efeitos dos fármacos
Dentina/efeitos dos fármacos
Antibacterianos/farmacologia
-Fatores de Tempo
Cânfora/farmacologia
Contagem de Colônia Microbiana
Clorofenóis/farmacologia
Reprodutibilidade dos Testes
Estatísticas não Paramétricas
Microscopia Confocal
Biofilmes/efeitos dos fármacos
Cavidade Pulpar/efeitos dos fármacos
Cavidade Pulpar/microbiologia
Dentina/microbiologia
Combinação de Medicamentos
Viabilidade Microbiana/efeitos dos fármacos
Limites: Seres Humanos
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-792589
Autor: WANG, Linda; BIM JÚNIOR, Odair; LOPES, Adolfo Coelho de Oliveira; FRANCISCONI-DOS-RIOS, Luciana Fávaro; MAENOSONO, Rafael Massunari; D’ALPINO, Paulo Henrique Perlatti; HONÓRIO, Heitor Marques; ATTA, Maria Teresa.
Título: Water interaction and bond strength to dentin of dye-labelled adhesive as a function of the addition of rhodamine B
Fonte: J. appl. oral sci;24(4):317-324, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: ABSTRACT Objective This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system. Material and Methods The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB. For the WS/WSL tests, cured resin disks (5.0 mm in diameter x 0.8 mm thick) were prepared and assigned into four groups (n=10): MP, MP-RB, SE, and SE-RB. For µTBS assessment, extracted human third molars (n=40) had the flat occlusal dentin prepared and assigned into the same experimental groups (n=10). After the bonding and restoration procedures, specimens were sectioned in rectangular beams, stored in water and tested after seven days or after 12 months. The failure mode of fractured specimens was qualitatively evaluated under optical microscope (x40). Data from WS/WSL and µTBS were assessed by one-way and three-way ANOVA, respectively, and Tukey’s test (α=5%). Results RB increased the WSL of MP and SE. On the other hand, WS of both MP and SE was not affected by the addition of RB. No significance in µTBS between MP and MP-RB for seven days or one year was observed, whereas for SE a decrease in the µTBS means occurred in both storage times. Conclusions RB should be incorporated into non-simplified DBSs with caution, as it can interfere with their physical-mechanical properties, leading to a possible misinterpretation of bonded interface.
Descritores: Rodaminas/química
Água/química
Adesivos Dentinários/química
Dentina/efeitos dos fármacos
Corantes Fluorescentes/química
-Solubilidade
Propriedades de Superfície
Resistência à Tração
Fatores de Tempo
Teste de Materiais
Reprodutibilidade dos Testes
Colagem Dentária/métodos
Microscopia Confocal
Resinas Compostas/química
Cimentos de Resina/química
Falha de Restauração Dentária
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Bramante, Clóvis Monteiro
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Id: lil-787545
Autor: TARTARI, Talita; BACHMANN, Luciano; MALIZA, Amanda Garcia Alves; ANDRADE, Flaviana Bombarda; DUARTE, Marco Antonio Hungaro; BRAMANTE, Clovis Monteiro.
Título: Tissue dissolution and modifications in dentin composition by different sodium hypochlorite concentrations
Fonte: J. appl. oral sci;24(3):291-298tab, graf.
Idioma: en.
Projeto: State of São Paulo Research Foundation; . State of São Paulo Research Foundation.
Resumo: ABSTRACT Sodium hypochlorite (NaOCl) remains the most used irrigation solution during root canal preparation because of characteristics such as wide-spectrum antimicrobial activity and organic tissue dissolution capacity. However, these solutions can alter dentin composition and there is no consensus on the optimal concentration of NaOCl to be used. Objectives To determine the organic matter dissolution and changes in dentin chemical composition promoted by different concentrations of NaOCl over time. Material and Methods: Fragments of bovine muscle tissue were weighed before and after 5, 10, and 15 min of immersion in the groups (n=10): G1- 0.9% saline solution; G2- 1% NaOCl; G3- 2.5% NaOCl; and G4- 5% NaOCl. Bovine dentin fragments were subjected to the same irrigants and absorption spectra were collected by Attenuated Total Reflectance of Fourier Transform Infrared Spectroscopy (ATR-FTIR) before and after 0,5, 1, 2, 3, 5, 8, and 10 min of immersion in the solutions. The ratios of the amide III/phosphate and carbonate/phosphate absorption bands were determined. The tissue dissolution and carbonate/phosphate ratios were submitted to the two-way analysis of variance (ANOVA) with Tukey’s multiple-comparison test (α<0.05) and to the one-way analysis of variance with Tukey’s (α<0.05). The amide III/phosphate ratio was analyzed by Friedman test (α<0.05) and the Kruskal-Wallis test with Dunn’s post-hoc (α<0.05). Results The increase in NaOCl concentration and contact time intensified the dissolution of organic matter and dentin collagen with reduction in the amide III/phosphate ratio. Significant differences between all groups (p<0.05) were observed in the dissolution of organic matter at 10 min and in the amide III/phosphate ratio between the saline solution and 5% NaOCl at 5 min. The carbonate/phosphate ratio decreased significantly in G2, G3, and G4 after 0,5 min of immersion (p<0.05), but more alterations did not occur in the subsequent periods (p>0.05). Intergroup differences were not observed in this ratio (p>0.05). Conclusions The increase in the exposure time and in the concentration of NaOCl solution lead to an increase in the tissue dissolution and dentin collagen deproteination. Furthermore, some carbonate ions are removed from the dentin inorganic phase by the NaOCl.
Descritores: Hipoclorito de Sódio/química
Dentina/efeitos dos fármacos
Dentina/química
Desinfetantes/química
-Valores de Referência
Irrigantes do Canal Radicular/química
Solubilidade/efeitos dos fármacos
Propriedades de Superfície/efeitos dos fármacos
Fatores de Tempo
Colágeno/efeitos dos fármacos
Espectroscopia de Infravermelho com Transformada de Fourier
Concentração de Íons de Hidrogênio
Imersão
Músculos/efeitos dos fármacos
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


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Turssi, Cecília Pedroso
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Id: lil-787537
Autor: CARVALHO, Carolina; FERNANDES, Fernando Pelegrim; FREITAS, Valeria da Penha; FRANÇA, Fabiana Mantovani Gomes; BASTING, Roberta Tarkany; TURSSI, Cecilia Pedroso; AMARAL, Flávia Lucisano Botelho.
Título: Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
Fonte: J. appl. oral sci;24(3):211-217tab, graf.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: ABSTRACT Objective Green tea extract has been advocated as a matrix metalloproteinase (MMP) inhibitor; however, its effect on bond durability to caries-affected dentin has never been reported. Thus, the aim of this in vitro study was to evaluate the effect of two MMP inhibitors (2% chlorhexidine and 2% green tea extract), applied after acid etching, on bond durability of an etch-and-rinse adhesive system to caries-affected dentin. Material and Methods Occlusal enamel was removed from third molars to expose the dentin surface, and the molars were submitted to a caries induction protocol for 15 days. After removal of infected dentin, specimens were conditioned with 37% phosphoric acid (15 seconds) and randomly divided into three groups, according to the type of dentin pretreatment (n=10): NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul, MN, USA) was applied according to the manufacturer's instructions, and composite resin restorations were built on the dentin. After 24 hours, at 37°C, the resin-tooth blocks were sectioned perpendicularly to the adhesive interface in the form of sticks (0.8 mm2 of adhesive area) and randomly subdivided into two groups according to when they were to be submitted to microtensile bond strength (μTBS) testing: immediately or 6 months after storage in distilled water. Data were reported in MPa and submitted to two-way ANOVA for completely randomized blocks, followed by Tukey’s test (α=0.05). Results After 24 hours, there was no significant difference in the μTBS of the groups. After 6 months, the GT group had significantly higher μTBS values. Conclusion It was concluded that the application of 2% green tea extract was able to increase bond durability of the etch-and-rinse system to dentin. Neither the application of chlorhexidine nor non-treatment (NT - control) had any effect on bond strength after water storage.
Descritores: Ácidos Fosfóricos/química
Chá/química
Clorexidina/química
Cárie Dentária/tratamento farmacológico
Cimentos Dentários/química
Dentina/efeitos dos fármacos
Anti-Infecciosos Locais/química
-Propriedades de Superfície
Resistência à Tração
Fatores de Tempo
Ataque Ácido Dentário/métodos
Teste de Materiais
Distribuição Aleatória
Reprodutibilidade dos Testes
Análise de Variância
Colagem Dentária/métodos
Adesivos Dentinários/química
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Buzalaf, Marilia Afonso Rabelo
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Id: lil-777356
Autor: Hannas, Angelica Reis; Kato, Melissa Thiemi; Cardoso, Cristiane de Almeida Baldini; Magalhães, Ana Carolina; Pereira, José Carlos; Tjäderhane, Leo; Buzalaf, Marília Afonso Rabelo.
Título: Preventive effect of toothpastes with MMP inhibitors on human dentine erosion and abrasion in vitro
Fonte: J. appl. oral sci;24(1):61-66, Jan.-Feb. 2016. graf.
Idioma: en.
Projeto: CNPq.
Resumo: ABSTRACT The use of gels and mouthrinses with MMP inhibitors (chlorhexidine, and green tea extract) was shown to prevent erosive wear. The aim of this study was to analyze the protective effect of toothpastes containing MMP inhibitors on dentine loss induced by erosion in vitro. Material and Methods Five groups each containing 12 specimens of human root dentine were prepared. The specimens were subjected to 1 min erosion by immersion in a cola drink, 4 times a day, for 5 d. Each day, after the first and last erosive challenges, the specimens were brushed for 15 s with a slurry of dentifrice and water (1:3) containing placebo, 1,100 ppm fluoride, 0.61% green tea extract, 0.12% chlorhexidine or 0.004% chlorhexidine (commercial toothpaste). Between the acid challenges, the specimens were stored in artificial saliva with remineralizing potential until the next treatment. Dentine loss was determined using profilometry. Data were analyzed using one-way ANOVA after log transform (p<0.05). Results The mean wear values (μm) were as follows: placebo 1.83±0.53; 0.61% green tea extract 1.00±0.21; fluoride 1.27±0.43; 0.12% chlorhexidine 1.19±0.30; and 0.004% chlorhexidine 1.22±0.46. There was a significant difference in wear between placebo and all the treatment toothpastes, which did not differ from each other. Conclusion The results suggest that toothpastes containing MMP inhibitors are as effective as those based on NaF in preventing dentine erosion and abrasion.
Descritores: Abrasão Dentária/prevenção & controle
Erosão Dentária/prevenção & controle
Cremes Dentais/química
Dentina/efeitos dos fármacos
Inibidores de Metaloproteinases de Matriz/química
-Saliva Artificial/química
Propriedades de Superfície/efeitos dos fármacos
Fatores de Tempo
Escovação Dentária
Teste de Materiais
Bebidas Gaseificadas
Distribuição Aleatória
Clorexidina/química
Análise de Variância
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Carvalho, Ricardo Marins de
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Id: lil-447795
Autor: Silva, Nelson Renato Franca Alves da; Calamia, Christine S; Coelho, Paulo G; Carrilho, Marcela Rocha de Oliveira; Carvalho, Ricardo Marins de; Caufield, Page; Thompson, Van P.
Título: Effect of 2 percent iodine disinfecting solution on bond strength to dentin
Fonte: J. appl. oral sci;14(6):399-404, Nov.-Dec. 2006. tab, ilus.
Idioma: en.
Projeto: CNPq.
Resumo: INTRODUCTION: Disinfection of dentin surfaces is desirable so long as it does not interfere with subsequent bonding of adhesive resins. OBJECTIVE: To test the null hypothesis that bond strengths to dentin are not affected by previous application of an iodine disinfecting solution. MATERIALS AND METHODS: Twenty-four extracted non-carious molars were selected. Occlusal enamel was removed producing a flat dentin substrate. Test teeth were all treated with 2 percent Iodine disclosing/disinfecting solution (I2DDS) for 20 sec and rinsed for 20 sec followed by the application of self- or total- etching bonding systems, generating five adhesive groups (n=3): Single Bond; ; Prime & Bond NT; Clearfil SE Bond; Opti-Bond Plus. The control groups (n=3 per adhesive) had no disclosing/disinfectant application prior to adhesive application. A 4-mm thick resin restoration was built up on each tooth for microtensile testing. Statistical analyses between experimental and control groups were performed by student's t-test (alpha= 0.05). RESULTS: In general, experimental groups (previously treated with I2DDS) showed significantly lower bond strength values when compared with their respective controls (p<0.05), except for group Prime &Bond I2 that did not significantly differ from its control (p>0.05). CONCLUSION: Acetone-base adhesive systems seem not to be affected by the application of I2DDS prior to etching and bonding procedures.

INTRODUÇÃO: A desinfecção das superfícies de dentina é desejada desde que não haja interferência na adesão dos agentes adesivos. OBJETIVO: Testar a hipótese nula de que a resistência adesiva não é afetada pela aplicação prévia de uma solução desinfetante de iodo. MATERIAL AND MÉTODO: Vinte e quatro molares hígidos foram selecionados. O esmalte oclusal destes dentes foi removido, e sobre as superfícies planas de dentina expostas foi aplicada da solução desinfetante de Iodo a 2 por cento (I2DDS), que permaneceu sobre a superfície por 20 s e foi lavada por 20 s com água deionizada. Sobre as superfícies desinfetadas foram aplicados um dos seguintes sistemas adesivos (n=3): Single Bond; Prime & Bond NT; Clearfil SE Bond; Opti-Bond Plus. Os grupos controle (n=3) não tiveram a supeficie de dentina tratada com I2DDS antes dos precedimentos adesivos. Em todos os grupos, após hibridização da dentina, foi construída uma "restauração" de resina composta com cerca de 4 mm de espessura. Após 7 dias de armazenagem em água destilada, os dentes foram secionados de modo a originarem espécimes a serem submetidos ao teste de microtração (palitos). Análise estatística para comparação dos dados foi realizada pelo teste t de student (alfa=0,05). RESULTADOS: De forma geral, os grupos experimentais (tratados com I2DDS) apresentraram resistência adesiva significativamente menor do que os respectivos grupos controle (p<0.05), exceção se fez apenas para o grupo P&BI2, que não diferiu significativamente de seu grupo controle P&BC (p>0.05). Assim, a hipótese nula deve ser rejeitada para os adesivos a base de etanol e/ou a base de água, mas aceita para o adesivo a base de acetona. CONCLUSÃO: O uso da solução experimental de iodo previamente à realização dos procedimentos adesivos afetou a efetividade da união à dentina apenas quando do emprego de sistemas adesivos a base de etanol e/ou água.
Descritores: Adesivos Dentinários
Dentina
Desinfetantes
Iodo
Sensibilidade da Dentina
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Id: lil-728294
Autor: Carrasco Tapia, Patricio; González Srur, Joaquín; Brizuela Cordero, Claudia; Inostroza Silva, Carolina.
Título: Estimación de la edad médico-legal usando dos métodos para la medición de la translucidez dentinaria radicular: análisis comparativo / Legal medical age estimation using two methods measuring root dentin translucency: comparative analysis
Fonte: Int. j. morphol;32(3):956-961, Sept. 2014. ilus.
Idioma: es.
Projeto: Universidad de los Andes. Facultad de Odontología Dirección de Investigación.
Resumo: En una investigación forense existen diversas incógnitas médico legales y criminalísticas por definir: especie, sexo, edad, identidad, causa y data de muerte de la víctima o victimario. Fenómenos biológicos han colaborado en estimar la edad médico legal de un individuo. Investigadores han propuesto diferentes métodos para esta estimación. El fenómeno de la translucidez dentinaria radicular se asocia con el aumento de la edad del individuo y ha demostrado ser de gran utilidad en esta estimación. El objetivo fue comparar el acierto en la estimación de la edad médico legal en tres rangos etarios predeterminados, mediante la medición de la translucidez dentinaria radicular, aplicando dos metodologías. Se realizaron mediciones de la longitud de la translucidez de piezas dentarias de una población chilena (n=94), mediante un método visual directo convencional y un método digital indirecto basado en una captura computarizada de la imagen. Se estimó la edad aplicando una tabla propuesta. Se preestablecieron tres rangos de edad: 1er rango (20-39 años), 2do rango (40­59 años) y 3er rango (60­79 años). El método digital muestra un acierto en la estimación de la edad en el 1er rango etario (93%), en comparación al método visual que muestra un acierto 2do y 3er rango (86% y 65%). Existen diferencias significativas p=0,001 entre ambos métodos para cada rango. La diferencia no es estadísticamente significativa en los porcentajes totales de aciertos en la estimación de la edad con ambos métodos. Enfrentados a la estimación de la edad en piezas dentarias con poca TDR se recomienda el método digital, considerando que tiende a una subestimación. En casos de mayor TDR recomendamos el método visual, que tiende a sobreestimar. Este trabajo demuestra que ambos métodos son igualmente efectivos.

In a forensic investigation there are several forensic and criminology unknowns to be elucidated, such as, species, sex, age, identity, cause and date of death of the victim or perpetrator. Some biological phenomena have been used to collaborate in determining the forensic age of an individual. Many authors and researchers have proposed different ways to perform this estimate. The phenomenon of root dentine translucency (RDT) is associated with the increase of age of the individual and has proven to be useful in the estimation of this unknown. The objective of this study was to compare the accuracy in estimating forensic age in three age ranges, by measurement of RDT, using two different methodologies. The protocol consists of taking measurements of the length translucency of dental pieces in a Chilean population (n=94), by direct visual method conventionally used and an indirect digital method based on the capture of a computerized image. The age was estimated applying a table: Three age ranges are preset: 1st range (20-39 ages), 2nd range (40-59 ages) and 3rd range (60-79 ages). The digital method gave better results in the estimation of age in the 1st age range (93%), compared to the visual method that was more assertive in the 2nd and 3rd range (86% and 65%). There are significant differences p=0.001 between the methods for each age range. The difference between the total percentages of successes in the age estimate obtained with both methods are not statistically significant. In conclusion, for age determination in teeth with very little translucency the digital method is recommended, although we must consider that this method tends to underestimate age. In cases of higher RDT we recommend using the visual method; however this method tends to overestimate age. Regardless of the above conclusion, this work shows that both methods are equally effective.
Descritores: Raiz Dentária/anatomia & histologia
Determinação da Idade pelos Dentes/métodos
Dentina/anatomia & histologia
Odontologia Legal/métodos
-Processamento de Imagem Assistida por Computador
Luz
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Idoso
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893665
Autor: Rocha, Fernanda Regina Godoy; Souza, João Antônio Chaves de; Guimarães-Stabili, Morgana Rodrigues; Sampaio, José Eduardo Cezar; Rossa Junior, Carlos.
Título: Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
Fonte: J. appl. oral sci;25(6):689-699, Nov.-Dec. 2017. graf.
Idioma: en.
Projeto: São Paulo State Foundation for Research Support (FAPESP).
Resumo: Abstract Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p<0.05) increased cell proliferation, except for BMSC grown on non-conditioned dentin slices. Dentin substrate discretely increased expression of Col1a1 in all cell types. Expression of Runx2, Col1a1 and Fn was either unaffected or inhibited by bFGF application in all cell types. We could not detect expression of the target genes on BMSC grown onto conditioned dentin. Conclusion: Acid conditioning of dentin improves the release of topically-applied bFGF. Topical application of bFGF had a stimulatory effect on proliferation of PDL fibroblasts, cementoblasts and BMSC, but did not affect expression of Runx2, Col1a1, Bglap and fibronectin by these cells.
Descritores: Ligamento Periodontal/efeitos dos fármacos
Regeneração/efeitos dos fármacos
Fator 2 de Crescimento de Fibroblastos/farmacologia
Dentina/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
-Ligamento Periodontal/metabolismo
Expressão Gênica
Fator 2 de Crescimento de Fibroblastos/administração & dosagem
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-893633
Autor: Fatemeh, KOOHPEIMA; Mohammad Javad, MOKHTARI; Samaneh, KHALAFI.
Título: The effect of silver nanoparticles on composite shear bond strength to dentin with different adhesion protocols
Fonte: J. appl. oral sci;25(4):367-373, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: Shiraz University of Medical Sciences.
Resumo: Abstract In Dentistry, restorative materials and oral bacteria are believed to be responsible for restoration failure. To make long-lasting restorations, antibacterial agents should be made. Inorganic nanoparticles and their nano composites are applied as good antibacterial agents. Objective The purpose of this study was to investigate the effect of silver nanoparticles on composite shear bond strength using one etch and rinse and one self-etch adhesive systems. Material and Methods Silver nanoparticles were prepared. Transmission electron microscope and X-ray diffraction were used to characterize the structure of the particles. Nanoparticles were applied on exposed dentin and then different adhesives and composites were applied. All samples were tested by universal testing machine and shear bond strength was assesed. Results Particles with average diameter of about 20 nm and spherical shape were found. Moreover, it was shown that pretreatment by silver nanoparticles enhanced shear bond strength in both etch and rinse, and in self-etch adhesive systems (p≤0.05). Conclusions Considering the positive antibacterial effects of silver nanoparticles, using them is recommended in restorative dentistry. It seems that silver nanoparticles could have positive effects on bond strength of both etch-and-rinse and self-etch adhesive systems. The best results of silver nanoparticles have been achieved with Adper Single Bond and before acid etching.
Descritores: Prata/química
Colagem Dentária/métodos
Cimentos de Resina/química
Cimentos Dentários/química
Dentina/efeitos dos fármacos
Nanopartículas Metálicas/química
-Valores de Referência
Prata/farmacologia
Propriedades de Superfície/efeitos dos fármacos
Ataque Ácido Dentário/métodos
Difração de Raios X/métodos
Teste de Materiais
Reprodutibilidade dos Testes
Análise de Variância
Resistência ao Cisalhamento/efeitos dos fármacos
Dentina/química
Microscopia Eletrônica de Transmissão/métodos
Antibacterianos/química
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME



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