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Coelho, Humberto Eustáquio
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Id: biblio-1012003
Autor: Magalhães, Fernanda Oliveira; Uber-Bucek, Elizabeth; Ceron, Patricia Ibler Bernardo; Name, Thiago Fellipe; Coelho, Humberto Eustáquio; Barbosa, Claudio Henrique Gonçalves; Carvalho, Tatiane; Groppo, Milton.
Título: Chronic treatment with hydroalcoholic extract of Plathymenia reticulata promotes islet hyperplasia and improves glycemic control in diabetic rats / Tratamento crônico com extrato hidroalcoólico de Plathymenia reticulata promove hiperplasia de ilhotas e controle glicêmico em ratos diabéticos
Fonte: Einstein (Säo Paulo);17(3):eAO4635, 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: To investigate the anti-hyperglycemic effects of Plathymenia reticulata hydroalcoholic extract and related changes in body weight, lipid profile and the pancreas. Methods: Diabetes was induced in 75 adult male Wistar rats via oral gavage of 65mg/Kg of streptozotocin. Rats were allocated to one of 8 groups, as follows: diabetic and control rats treated with water, diabetic and control rats treated with 100mg/kg or 200mg/kg of plant extract, and diabetic and control rats treated with glyburide. Treatment consisted of oral gavage for 30 days. Blood glucose levels and body weight were measured weekly. Animals were sacrificed and lipid profile and pancreatic tissue samples analyzed. Statistical analysis consisted of ANOVA, post-hoc Tukey-Kramer, paired Student's t and χ2 tests; the level of significance was set at 5%. Results: Extract gavage at 100mg/kg led to a decrease in blood glucose levels in diabetic rats in the second, third (198.71±65.27 versus 428.00±15.25) and fourth weeks (253.29±47.37 versus 443.22±42.72), body weight loss (13.22±5.70 versus 109.60±9.95) and lower cholesterol levels (58.75±3.13 versus 80.11±4.01) in control rats. Extract gavage at 200mg/Kg led to a decrease in glucose levels on the fourth week in diabetic rats, body weight loss in the second, third and fourth weeks in control rats, and lower cholesterol levels in diabetic and control rats. Islet hyperplasia (p=0.005) and pancreatic duct dilation (p=0.047) were observed in diabetic and control rats. Conclusion: Plathymenia extract reduced blood glucose levels in diabetic rats, and body weight in control rats, and promoted pancreatic islet hyperplasia in diabetic and control rats.

RESUMO Objetivo: Avaliar o efeito anti-hiperglicêmico do extrato hidroalcoólico de Plathymenia reticulata, alterações no peso, lipídeos e efeito sobre o pâncreas. Métodos: O diabetes foi induzido pela administração de estreptozotocina 65mg/kg, em 75 ratos Wistar adultos machos, divididos em 8 grupos diferentes: ratos diabéticos e controle + água, ratos diabéticos e controle + 100mg/kg ou 200mg/kg de extrato, ratos diabéticos e controle + gliburida. O tratamento foi realizado por gavagem (oral) por 30 dias. Níveis de glicose e peso foram verificados semanalmente. Os animais foram sacrificados, e amostras de lipídeos e do pâncreas foram analisadas. A análise estatística incluiu ANOVA, post-hoc Tukey-Kramer, teste t de Student pareado e teste do χ2, com nível de significância de 5%. Resultados: O extrato 100mg/kg promoveu redução nos níveis de glicose sanguínea em ratos diabéticos na segunda, terceira (198,71±65,27 versus 428,00±15,25) e quarta semanas (253,29±47,37 versus 443,22±42,72), perda de peso (13,22±5,70 versus 109,60±9,95) e diminuição do colesterol (58,75±3,13 versus 80,11±4,01) em ratos controle. Com extrato de 200mg/kg, houve redução dos níveis de glicose na quarta semana, nos ratos diabéticos; de peso na segunda, terceira e quarta semanas, nos ratos controle; e de colesterol nos animais diabéticos e controle. Ocorreram hiperplasia de ilhotas (p=0,005) e dilatação dos ductos pancreáticos (p=0,047) em ratos diabéticos e controles. Conclusão: O extrato de Plathymenia reduziu os níveis de glicose em ratos diabéticos e de peso em ratos controle, além de ter promovido hiperplasia de ilhotas pancreáticas em diabéticos e controles.
Descritores: Extratos Vegetais/farmacologia
Diabetes Mellitus Experimental/tratamento farmacológico
Hipoglicemiantes/farmacologia
Fabaceae
-Glicemia/análise
Peso Corporal/efeitos dos fármacos
Colesterol
Ratos Wistar
Estreptozocina
Folhas de Planta
Diabetes Mellitus Experimental/induzido quimicamente
Modelos Animais de Doenças
Hiperplasia/patologia
Fitoterapia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1047771
Autor: Yan, Xia; Qian, Chaoju; Yin, Xiaoyue; Fan, Xingke; Zhao, Xueyong; Gu, Menghe; Wang, Tao; Ma, Xiao-Fei.
Título: A whole-transcriptome approach to evaluate reference genes for quantitative diurnal gene expression studies under natural field conditions in Tamarix ramosissima leaves
Fonte: Electron. j. biotechnol;35:48-56, sept. 2018. tab, graf.
Idioma: en.
Projeto: National Key Research and Development Program of China; . Natural Science Foundation of China; . Gansu Science and Technology Supporting Project.
Resumo: Background: Tamarix ramosissima is a desert forest tree species that is widely distributed in the drought-stricken areas to sustain the fragile ecosystem. Owing to its wide usage in the desert restoration of Asia, it can be used as an ecophysiological model plant. To obtain reliable and accurate results, a set of reference genes should be screened before gene expression. However, up to date, systematical evaluation of reference genes has not been conducted in T. ramosissima. Results: In this study, we used eigenvalues derived from principal component analysis to identify stable expressed genes from 72,035 unigenes from diurnal transcriptomes under natural field conditions. With combined criteria of read counts above 900 and CV of FPKM below 0.3, a total of 7385 unigenes could be qualified as candidate reference genes in T. ramosissima. By using three statistical algorithm packages, geNorm, NormFinder, and BestKeeper, the stabilities of these novel reference genes were further compared with a panel of traditional reference genes. The expression patterns of three aquaporins (AQPs) suggested that at least UBQ (high expression), EIF4A2 (low expression), and GAPDH (moderate expression) could be qualified as ideal reference genes in both RT-PCR and RNA-seq analysis of T. ramosissima. Conclusions: This work will not only facilitate future studies on gene expression and functional analysis of genetic resources of desert plants but also improve our understanding of the molecular regulation of water transport in this plant, which could provide a new clue to further investigate the drought adaptation mechanism of desert plant species under harsh environments.
Descritores: Tamaricaceae/genética
Transcriptoma
-Padrões de Referência
Adaptação Biológica
Expressão Gênica
Ecossistema
Folhas de Planta/genética
Deserto
Recuperação e Remediação Ambiental
Secas
Reação em Cadeia da Polimerase em Tempo Real
RNA-Seq
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1253093
Autor: Umer, Noroza; Zahra Naqvi, Rubab; Rauf, Imran; Anjum, Naveed; Keen, Patricia R; Van Eck, Joyce; Jander, Georg; Asif, Muhammad.
Título: Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae
Fonte: Electron. j. biotechnol;47:72-82, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: International Research Support Initiative Program of Higher Education Commission (HEC) of; . Pakistan; . United States Department of Agriculture (USDA).
Resumo: BACKGROUND: Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plantderived insect resistance genes is a promising approach to control aphids and other sap-sucking insect pests. While expression from the constitutive 35S promoter provides broad protection, the phloem-specific rolC promoter provides better defense against sap sucking insects. The selection of plant-derived insect resistance genes for expression in crop species will minimize bio-safety concerns. RESULTS: Pinellia ternata leaf agglutinin gene (pta), encodes an insecticidal lectin, was isolated and cloned under the 35S and rolC promoters in the pGA482 plant transformation vector for Agrobacterium-mediated tobacco transformation. Integration and expression of the transgene was validated by Southern blotting and qRT-PCR, respectively. Insect bioassays data of transgenic tobacco plants showed that expression of pta under rolC promoter caused 100% aphid mortality and reduced aphid fecundity up to 70% in transgenic tobacco line LRP9. These results highlight the better effectivity of pta under rolC promoter to control phloem feeders, aphids. CONCLUSIONS: These findings suggested the potential of PTA against aphids and other sap sucking insect pests. Evaluation of gene in tobacco under two different promoters; 35S constitutive promoter and rolC phloemspecific promoter could be successfully use for other crop plants particularly in cotton. Development of transgenic cotton plants using plant-derived insecticidal, PTA, would be key step towards commercialization of environmentally safe insect-resistant crops.
Descritores: Afídeos/patogenicidade
Controle Biológico de Vetores
Pinellia/química
-Vírus de Plantas
Tabaco
Southern Blotting
Reação em Cadeia da Polimerase
Regiões Promotoras Genéticas
Plantas Geneticamente Modificadas
Folhas de Planta/química
Transgenes
Resistência à Doença
Proteção de Cultivos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950715
Autor: Sytar, Oksana; Borankulova, Asel; Hemmerich, Irene; Rauh, Cornelia; Smetanska, Iryna.
Título: Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants
Fonte: Biol. Res;47:1-7, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. RESULTS: Treatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids - vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant organs but also between early stages of vegetation period. Same changes as in contents of chlorogenic acid, ferulic acid, trans-ferulic acid were found for content of salycilic acid. The content of these phenolic acids has been significant increased under effect of 2% CCC treatment at the phase I (formation of buds) in the stems and at the phase II (beginning of flowering) in the leaves and then inflorescences respectively. The content of catechins as potential buckwheat antioxidants has been increased at the early stages of vegetation period after treatment with 2% CCC. CONCLUSIONS: The obtained results suggest that influence of CCC on the phenolics composition can be a result of various mechanisms of CCC uptake, transforming and/or its translocation in the buckwheat seedlings.
Descritores: Clormequat/farmacologia
Fagopyrum/efeitos dos fármacos
Polifenóis/biossíntese
Hidroxibenzoatos/metabolismo
-Propionatos
Sementes/efeitos dos fármacos
Sementes/metabolismo
Catequina/análise
Ácido Clorogênico/análise
Cromatografia Líquida de Alta Pressão
Compostos de Tungstênio
Caules de Planta/efeitos dos fármacos
Caules de Planta/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Fagopyrum/crescimento & desenvolvimento
Fagopyrum/metabolismo
Ácidos Cumáricos/análise
Inflorescência/efeitos dos fármacos
Inflorescência/metabolismo
Éteres de Hidroxibenzoatos/análise
Hidroxibenzoatos/química
Molibdênio
Antioxidantes/análise
Antioxidantes/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950725
Autor: Slimane, Badreddine Ben; Ezzine, Olfa; Dhahri, Samir; Jamaa, Mohamed Lahbib Ben.
Título: Essential oils from two Eucalyptus from Tunisia and their insecticidal action on Orgyia trigotephras (Lepidotera, Lymantriidae)
Fonte: Biol. Res;47:1-8, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Essential oils extracted from aromatic and medicinal plants have many biological properties and are therefore an alternative to the use of synthetic products. The chemical composition of essential oils from two medicinal plants (Eucalyptus globulus and E. lehmannii) was determined and, their insecticidal effects on the third and fourth larval stages of Orgyia trigotephras were assessed. RESULTS: Larvae were collected from Jebel Abderrahmane (North-East of Tunisia), conserved in groups of 50/box (21 × 10 × 10 cm) at a temperature of 25°C. Larvae were tested for larvicidal activities of essential oils. Each oil was diluted in ethanol (96%) to prepare 3 test solutions (S1 = 0.05%, S2 = 0.10% and S3 = 0.50%). Essential oils were used for contact, ingestion and Olfactory actions and compared to reference products (Bacillus thuringiensis and Decis). Olfactory action of essential oils shows that larvae mortality is higher than contact action, lower than ingestion action. MTM and FTM of S3 of E. lehmannii were respectively 1 h 32 min and 1 h 39 min are higher than those of E. globulus (MTM = 51 min and FTM = 1 h 22 min 34 sec). Contact action of E. lehmannii oil shows low insecticidal activity compared to E. globulus. MTM are respectively (1 min 52 sec and 1 min 7 sec), FTM are (2 min 38 sec, 1 min 39 sec), are the shortest recorded for S3, on the third stage of larvae. The fourth stage of larvae, MTM are (2 min 20 sec and 2 min 9 sec), FTM are (3 min 25 sec, 3 min 19 sec). Ingestion action of essential oils is longer than the contact action, since the time of death exceeds 60 minutes for all species. CONCLUSION: Results shows that essential oils have a toxic action on nerves leading to a disruption of vital system of insects. High toxic properties make these plant-derived compounds suitable for incorporation in integrated pest management programs.
Descritores: Óleos Voláteis/química
Eucalyptus/química
Inseticidas
Lepidópteros
-Tunísia
Cromatografia Gasosa
Folhas de Planta/metabolismo
Folhas de Planta/química
Cicloexanóis/análise
Monoterpenos/análise
Eucalyptus/classificação
Agentes de Controle Biológico
Herbivoria
Eucaliptol
Monoterpenos Bicíclicos
Larva/efeitos dos fármacos
Estágios do Ciclo de Vida/efeitos dos fármacos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950728
Autor: Castro, Jenny P; Ocampo, Yanet C; Franco, Luis A.
Título: In vivo and in vitro anti-inflammatory activity of Cryptostegia grandiflora Roxb. ex R. Br. leaves
Fonte: Biol. Res;47:1-8, 2014. ilus, graf, tab.
Idioma: en.
Projeto: University of Cartagena.
Resumo: BACKGROUND: Despite Cryptostegia grandiflora Roxb. ex R. Br. (Apocynaceae) leaves are widely used in folk Caribbean Colombian medicine for their anti-inflammatory effects, there are no studies that support this traditional use. Therefore, this work aimed to evaluate the effect of the total extract and primary fractions obtained from Cryptostegia grandiflora leaves, using in vivo and in vitromodels of inflammation, and further get new insights on the mechanisms involved in this activity. RESULTS: Ethanolic extract of Cryptostegia grandiflora leaves, and its corresponding ether and dichloromethane fractions, significantly reduced inflammation and myeloperoxidase activity (MPO) in ear tissue of mice treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Histological analysis revealed a reduction of edema and leukocyte infiltration. Complementarily, we demonstrated that extract and fractions reduced nitric oxide (NO•) and prostaglandin E2 (PGE2) production in LPS-stimulated RAW 264.7 macrophages, as well as scavenging activity on DPPH and ABTS radicals. CONCLUSIONS: Our results demonstrated for the first time the anti-inflammatory activity of Cryptostegia grandiflora leaves, supporting its traditional use. This activity was related to inhibition of MPO activity, and PGE2 and NO• production. These mechanisms and its antioxidant activity could contribute, at least in part, to the anti-inflammatory effect showed by this plant.
Descritores: Extratos Vegetais/uso terapêutico
Apocynaceae/química
Edema/tratamento farmacológico
Macrófagos/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
-Ocitócicos/análise
Dinoprostona/análise
Peroxidase/antagonistas & inibidores
Folhas de Planta/química
Citotoxinas/farmacologia
Linhagem Celular Tumoral/efeitos dos fármacos
Inflamação/tratamento farmacológico
Camundongos Endogâmicos ICR
Óxido Nítrico/análise
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950733
Autor: Cetin, Emine Sema.
Título: Induction of secondary metabolite production by UV-C radiation in Vitis vinifera L. Öküzgözü callus cultures
Fonte: Biol. Res;47:1-7, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: The aim of the present work was to examine the role of UV-C irradiation on the production of secondary metabolites (total phenolic, total flavanols, total flavonols, catechin, ferulic acid and trans-resveratrol in phenolic compounds and α-, ß-, γ- δ-tocopherols) in callus cultures. Studies on the effects of UV-C treatment on callus culture are seldom and generally focused on UV-B. However UV-C radiation play an important role in accumule secondary metabolites. RESULTS: In this study, callus cultures from Öküzgözü grape cultivar were initiated from leaf petiole explants. Calli formed after 6 weeks on the medium supplemented with 0.5 mg L-1 benzylaminopurine (BA), 0.5 mg L-1 indole acetic acid (IAA) on B5 media. Callus tissues were exposed to UV-C irradiation at 10, 20 and 30 cm distances from the UV source for 5 and 10 minutes and samples were collected at hours 0, 24 and 48. CONCLUSIONS: The greatest total phenolic content (155.14 mg 100 g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. 24 h and 48 h incubation times, 30 cm and 5 min were the most appropriate combination of UV-C application in total flavanol content. Maximum total flavonol content (7.12 mg 100 g-1) was obtained on 0 h, 5 min and 20 cm combination. The highest (+)- catechin accumulation (8.89 mg g-1) was found in calli with 10 min UV-C application from 30 cm distance and sampled after 48 h. Ferulic acid content increased 6 fold in Öküzgözü callus cultures (31.37 µg g-1) compared to the control group. The greatest trans-resveratrol content (8.43 µg g-1) was detected in calli exposed to UV-C for 5 min from 30 cm distance and sampled after 24 h. The highest α-tocopherol concentration was found in calli exposed to UV-C for 10 min from 30 cm distance and sampled after 24 h. As a conclusion, it was showed that UV-C radiation had remarkable promoting effects on the accumulation of secondary metabolites in the calli of Öküzgözü grape cultivar.
Descritores: Raios Ultravioleta
Folhas de Planta/efeitos da radiação
Produtos Agrícolas/efeitos da radiação
Vitis/efeitos da radiação
Técnicas de Embriogênese Somática de Plantas/métodos
Metabolismo Secundário/efeitos da radiação
-Fenóis/análise
Estilbenos/análise
Flavonoides/análise
Catequina/análise
Cromatografia
Folhas de Planta/metabolismo
Folhas de Planta/química
Produtos Agrícolas/fisiologia
Ácidos Cumáricos/análise
Vitis/metabolismo
Vitis/química
Tocoferóis/análise
Flavonóis/análise
Metabolismo Secundário/fisiologia
Resveratrol
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-868825
Autor: Nereida, Marroquín; Cruz, Sully M.
Título: Actividad biológica y caracterización química de los extractos de las hojas y corteza de Rhizophora mangle L / Biological activity and chemical characterization of leaves and bark extracts of Rhizophora mangle L
Fonte: Cienc. tecnol. salud;3(1):55-64, ene.-jun. 2016. tab.
Idioma: es.
Resumo: La corteza de Rhizophora mangle L., mangle rojo, ha sido utilizado tradicionalmente por sus propiedades como antiséptico, astringente y hemostático, se ha descrito la presencia de polifenoles como flavonoides y taninos, a los cuales se les ha relacionado con su acción antioxidante y cicatrizante demostrada en diferentes estudios; por su parte las hojas han presentado taninos y actividad antioxidante muy similar, y en ocasiones superior, a la reportada para la corteza. En este estudio se determinó la cantidad de taninos, flavonoides, actividad antioxidante y antibacteriana de cinco extractos etanólicos de hoja y corteza de mangle rojo, colectado en cinco transectos de la Reserva Natural de Usos Múltiples Monterrico Santa Rosa, Guatemala; se analizaron los datos con base en los promedios y desviaciones estándar de cada uno de los parámetros evaluados. En los extractos de hojas se determinó un 15.91±8.56% de taninos, 315.19±90.83ppm de flavonoides, actividad antioxidante a una concentración inhibitoria media (CI50) de 0.435±0.315mg/mL, 125.44±65.05μg de ácido gálico/g de extracto y actividad antibacterianacontra Staphylococcus aereus ATCC 6538, S. epidermidis ATCC 14990 y S. epidermidis aislada de herida con unaconcentración mínima inhibitoria (CIM) de 1 mg/mL; siendo estos datos similares a los de corteza. Se obtuvo uncoeficiente de correlación de -.79, entre la cantidad de taninos y actividad antioxidante (p < .001); lo cual relaciona su composición química con su posible efecto cicatrizante, por lo que los extractos de hoja pueden constituir unaalternativa viable para el desarrollo de productos naturales.

The Rhizophora mangle L. cortex, red mangrove, has a traditional use due to its antiseptic, astringent and hemostaticproperties; it has been described the content of polyphenols mainly as flavonoids and tannins, withantioxidant and wound healing properties, demonstrated in various studies, meanwhile the leafs presents tannins and similar antioxidant activity, sometimes, superior to the cortex activity. In this study, the amount of tannins, flavonoids, antioxidant and antibacterial activity of five ethanol extracts of leaf and cortex of red mangrove were determined; the samples were collected in the nature reserve multipurpose Monterric o, Santa Rosa, Guatemala; the data was analyzed based on the averages and standard deviations of each of the parameters evaluated. Inleaf extracts were quantified 15.91±8.56% of tannins, flavonoids 315.19±90.83 ppm, antioxidant activity of IC50 0.435±0.315mg/mL, total phenols of 125.44±65.05 μg gallic acid/ g of extract and antibacterial activity against Staphylococcus aureus ATCC 6538, S. epidermidis ATCC 14990 and S. salvaje with a MIC of 1 mg/mL, beingcomparables as those of the cortex. A correlation of -.79 was obtained, between the amount of tannins and antioxidant activity (p <.001); relating the results to a possible biological wound healing effects, consequently the leafextracts can become a viable option to the development of medicinal natural products.
Descritores: Antibacterianos
Antioxidantes
Folhas de Planta
Áreas Alagadas
Responsável: GT49.1


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Id: biblio-950741
Autor: Hossain, Mohammad Mobarak; Ahamed, Sayed Koushik; Dewan, Syed Masudur Rahman; Hassan, Md Mahadi; Istiaq, Arif; Islam, Mohammad Safiqul; Moghal, Md Mizanur Rahman.
Título: In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthes paniculata leaves
Fonte: Biol. Res;47:1-9, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: The study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of Spilanthes paniculata leaves for the first time widely used in the traditional treatments in Bangladesh. RESULTS: In antipyretic activity assay, a significant reduction (P < 0.05) was observed in the temperature in the mice tested. At dose 400 mg/kg-body weight, the n-hexane soluble fraction showed the effect (36.7 ± 0.63°C ) as like as the standard (dose 150 mg/kg-body weight) after 5 h of administration. Extracts showed significant (P < 0.001) potential when tested for the antiemetic activity compared to the standard, metoclopramide. At dose 50 mg/kg-body weight, the standard showed 67.23% inhibition, whereas n-hexane and ethyl acetate soluble fractions showed 37.53% and 24.93% inhibition of emesis respectively at dose 400 mg/kg-body weight. In antimicrobial activity assay, the n-hexane soluble fraction (400 µg/disc) showed salient activity against the tested organisms. It exerts highest activity against Salmonella typhi (16.9 mm zone of inhibition); besides, crude, and ethyl acetate extracts showed resistance to Bacillus cereus and Bacillus subtilis, and Vibrio cholera respectively. All the extracts were tested for lysis of the erythrocytes. At the concentration of 1mg/ml, ethanol extract, and n-hexane and ethyl acetate soluble fractions significantly inhibited hypotonic solution induced lysis of the human red blood cell (HRBC) (27.406 ± 3.57, 46.034 ± 3.251, and 30.72 ± 5.679% respectively); where standard drug acetylsalicylic acid (concentration 0.1 mg/ml) showed 77.276 ± 0.321% inhibition. In case of heat induced HRBC hemolysis, the plant extracts also showed significant activity (34.21 ± 4.72, 21.81 ± 3.08, and 27.62 ± 8.79% inhibition respectively). In the brine shrimp lethality bioassay, the n-hexane fraction showed potent (LC50 value 48.978 µg/ml) activity, whereas ethyl acetate fraction showed mild (LC50 value 216.77 µg/ml) cytotoxic activity. CONCLUSIONS: Our results showed that the n-hexane extract has better effects than the other in all trials. In the context, it can be said that the leaves of S. paniculata possess remarkable pharmacological effects, and justify its folkloric use as antimicrobial, antipyretic, anti-inflammatory, and antiemetic agent. Therefore, further research may be suggested to find possible mode of action of the plant part.
Descritores: Asteraceae/química
Citotoxinas/farmacologia
Membrana Eritrocítica/efeitos dos fármacos
Antipiréticos/farmacologia
Antibacterianos/farmacologia
Antieméticos/farmacologia
-Artemia/efeitos dos fármacos
Salmonella typhi/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
Bacillus cereus/efeitos dos fármacos
Bacillus subtilis/efeitos dos fármacos
Vibrio cholerae/efeitos dos fármacos
Bioensaio/mortalidade
Extratos Vegetais/farmacologia
Testes de Sensibilidade Microbiana
Galinhas
Folhas de Planta/química
Asteraceae/classificação
Etanol
Membrana Eritrocítica/fisiologia
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Temperatura Alta
Hexanos
Medicina Tradicional
Acetatos
Limites: Humanos
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950743
Autor: Cetin, Emine Sema; Babalik, Zehra; Hallac-Turk, Filiz; Gokturk-Baydar, Nilgun.
Título: The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures
Fonte: Biol. Res;47:1-6, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Plant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell suspension cultures of grapevine. Cell suspensions initiated from callus belonging to petiole tissue was used as a plant material. Cadmium chloride was applied to cell suspension cultures in different concentration (1.0 mM and 1.5 mM) to enhance secondary metabolite (total phenolics, total flavanols, total flavonols, trans-resveratrol, and α-, ß-, γ- δ-tocopherols) production. Cells were harvested at two days intervals until the 6th day of cultures. Amounts of total phenolics, total flavanols and total flavonols; trans-resveratrol and tocopherols (α-, ß-, γ- and δ-tocopherols) and dry cell weights were determined in the harvested cells. RESULTS: Phenolic contents were significantly affected by the sampling time and cadmium concentrations. The highest values of total phenolic (168.82 mg/100 g), total flavanol (15.94 mg/100 g), total flavonol (14.73 mg/100 g) and trans-resveratrol (490.76 µg/100 g) were found in cells treated with 1.0 mM CdCl2 and harvested at day 2. Contents of tocopherols in the cells cultured in the presence of 1.0 mM CdCl2 gradually increased during the culture period and the highest values of α, ß and γ tocopherols (145.61, 25.52 and 18.56 µg/100 g) were detected in the cell cultures collected at day 6. CONCLUSIONS: As a conclusion, secondary metabolite contents were increased by cadmium chloride application and sampling time, while dry cell weights was reduced by cadmium chloride treatments.
Descritores: Cloreto de Cádmio/farmacologia
Vitis/efeitos dos fármacos
Cultura Primária de Células/métodos
Metabolismo Secundário/efeitos dos fármacos
-Fenóis/análise
Estilbenos/análise
Flavonoides/análise
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Folhas de Planta/química
Vitis/crescimento & desenvolvimento
Vitis/metabolismo
Vitis/química
Tocoferóis/análise
Flavonóis/análise
Proliferação de Células/efeitos dos fármacos
Técnicas de Embriogênese Somática de Plantas/métodos
Resveratrol
Responsável: CL1.1 - Biblioteca Central



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