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Pesquisa : A18.550 [Categoria DeCS]
Referências encontradas : 131 [refinar]
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Id: biblio-974331
Autor: Guo, Ruiting; Wang, Zhiying; Huang, Ying; Fan, Haijuan; Liu, Zhihua.
Título: Biocontrol potential of saline- or alkaline-tolerant Trichoderma asperellum mutants against three pathogenic fungi under saline or alkaline stress conditions
Fonte: Braz. j. microbiol;49(supl.1):236-245, 2018. graf.
Idioma: en.
Projeto: National High Technology Research and Development Program (the 13th Five-Year Plan Program); . Fundamental Research Funds for the Central Universities.
Resumo: ABSTRACT Salinity and alkalinity are major abiotic stresses that limit growth and development of poplar. We investigated biocontrol potential of saline- and alkaline-tolerant mutants of Trichoderma asperellum to mediate the effects of salinity or alkalinity stresses on Populus davidiana × P. alba var. pyramidalis (PdPap poplar) seedlings. A T-DNA insertion mutant library of T. asperellum was constructed using an Agrobacterium tumefaciens mediated transformation system; this process yielded sixty five positive transformants (T1-T65). The salinity tolerant mutant, T59, grew in Potato Dextrose Agar (PDA) containing up to 10% (1709.40 mM) NaCl. Under NaCl-rich conditions, T59 was most effective in inhibiting Alternaria alternata (52.00%). The alkalinity tolerant mutants, T3 and T5, grew in PDA containing up to 0.4% (47.62 mM) NaHCO3. The ability of the T3 and T5 mutants to inhibit Fusarium oxysporum declined as NaHCO3 concentrations increased. NaHCO3 tolerance of the PdPap seedlings improved following treatment with the spores of the WT, T3, and T5 strains. The salinity tolerant mutant (T59) and two alkalinity tolerant mutants (T3 and T5) generated in this study can be applied to decrease the incidence of pathogenic fungi infection under saline or alkaline stress.
Descritores: Doenças das Plantas/microbiologia
Trichoderma/fisiologia
Cloreto de Sódio/metabolismo
Populus/crescimento & desenvolvimento
Álcalis/metabolismo
Alternaria/fisiologia
Antibiose
-Doenças das Plantas/prevenção & controle
Estresse Fisiológico
Trichoderma/genética
Populus/microbiologia
Plântulas/crescimento & desenvolvimento
Plântulas/microbiologia
Responsável: BR1.1 - BIREME


  2 / 131 LILACS  
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Id: biblio-828195
Autor: Jadon, Kuldeep Singh; Shah, Rakesh; Gour, Hari Narayan; Sharma, Pankaj.
Título: Management of blight of bell pepper (Capsicum annuum var. grossum) caused by Drechslera bicolor
Fonte: Braz. j. microbiol;47(4):1020-1029, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: Abstract Sweet or bell pepper is a member of the Solanaceae family and is regarded as one of the most popular and nutritious vegetable. Blight, in the form of leaf and fruit blight, has been observed to infect bell pepper crops cultivated at the horticulture farm in Rajasthan College of Agriculture, Udaipur, India. Based on disease severity, we attempted to curb this newly emerged problem using different fungicides, plant extracts, bio-control agents, and commercial botanicals against the fungus in laboratory and pot experiments. Bio-control agent Trichoderma viride and plant growth promoting Rhizobacteria (PGPR) isolate Neist-2 were found to be quite effective against bell pepper blight. All evaluated fungicides, botanicals, commercial botanicals, and bio-control agents in vitro were further studied as seed dressers and two foliar sprays at ten days interval in pot experiments. The combinations of Vitavax, PGPR isolate Neist-2, and Mehandi extract were found to be very effective against bell pepper blight followed by Vitavax, T. viride, and Mehandi extract used individually. All treatments in the pot experiments were found to significantly reduce seedling mortality and enhance plant biomass of bell pepper. Thus, these experimental findings suggest that a better integrated management of bell pepper blight could be achieved by conducting field trials in major bell pepper- and chilli-cultivated areas of the state. Besides fungicides, different botanicals and commercial botanicals also seem to be promising treatment options. Therefore, the outcome of the present study provides an alternate option of fungicide use in minimizing loss caused by Drechslera bicolor.
Descritores: Doenças das Plantas/microbiologia
Ascomicetos
Capsicum/microbiologia
-Ascomicetos/efeitos dos fármacos
Capsicum/química
Extratos Vegetais/farmacologia
Extratos Vegetais/química
Testes de Sensibilidade Microbiana
Plântulas/efeitos dos fármacos
Antifúngicos/farmacologia
Antifúngicos/química
Responsável: BR1.1 - BIREME


  3 / 131 LILACS  
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Id: biblio-839361
Autor: Torres-Arias, Yamir; Fors, Rosalba Ortega; Nobre, Camila; Gómez, Eduardo Furrazola; Berbara, Ricardo Luis Louro.
Título: Production of native arbuscular mycorrhizal fungi inoculum under different environmental conditions
Fonte: Braz. j. microbiol;48(1):87-94, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract In order to obtain an arbuscular mycorrhizal fungi (AMF) native inoculum from Sierra de Moa and determine the most appropriate conditions for its big scale production, four light and temperature combinations were tested in three plant species (Calophyllum antillanum, Talipariti elatum and Paspalum notatum). Growth and development parameters, as well as the mycorrhizal functioning of the seedlings were evaluated. The natural light treatment under high temperatures (L-H) was the most suitable for the growth and development of the three plant species, showing the highest total biomass values, mainly of root, and a positive root-shoot ratio balance. This treatment also promoted higher values of root mycorrhizal colonization, external mycelium and AMF spore density. A total of 38 AMF species were identified among the plants and environmental conditions tested. Archaeospora sp.1, Glomus sp.5, Glomus brohultii and G. glomerulatum were observed in all the treatments. The L-H condition can be recommended for native inoculum production, as it promotes a better expression of the AM symbiosis and an elevated production of mycorrhizal propagules.
Descritores: Raízes de Plantas/microbiologia
Micorrizas
Meio Ambiente
-Microbiologia do Solo
Esporos Fúngicos
Simbiose
Contagem de Colônia Microbiana
Micorrizas/crescimento & desenvolvimento
Plântulas/crescimento & desenvolvimento
Plântulas/microbiologia
Responsável: BR1.1 - BIREME


  4 / 131 LILACS  
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Id: biblio-1022023
Autor: Darqui, Flavia S; Radonic, Laura M; López, Nilda; Hopp, H Esteban; López Bilbao, Marisa.
Título: Simplified methodology for large scale isolation of homozygous transgenic lines of lettuce
Fonte: Electron. j. biotechnol;31:1-9, Jan. 2018. ilus, graf, tab.
Idioma: en.
Projeto: (INTA).
Resumo: Background: Lettuce is a globally important leafy vegetable and a model plant for biotechnology due to its adaptability to tissue culture and stable genetic transformation. Lettuce is also crucial for functional genomics research in the Asteraceae which includes species of great agronomical importance. The development of transgenic events implies the production of a large number of shoots that must be differentiated between transgenic and non-transgenic through the activity of the selective agent, being kanamycin the most popular. Results: In this work we adjusted the selection conditions of transgenic seedlings to avoid any escapes, finding that threshold concentration of kanamycin was 75 mg/L. To monitor the selection system, we studied the morphological response of transgenic and non-transgenic seedlings in presence of kanamycin to look for a visual morphological marker. Several traits like shoot length, primary root length, number of leaves, fresh weight, and appearance of the aerial part and development of lateral roots were affected in non-transgenic seedlings after 30 d of culture in selective media. However, only lateral root development showed an early, qualitative and reliable association with nptII presence, as corroborated by PCR detection. Applied in successive transgenic progenies, this method of selection combined with morphological follow-up allowed selecting the homozygous presence of nptII gene in 100% of the analyzed plants from T2 to T5. Conclusions: This protocol allows a simplified scaling-up of the production of multiple homozygous transgenic progeny lines in the early generations avoiding expensive and time-consuming molecular assays.
Descritores: Plantas Geneticamente Modificadas/genética
Alface/genética
-Seleção Genética
Canamicina/análise
Reação em Cadeia da Polimerase
Alface/química
Plântulas
Homozigoto
Responsável: CL1.1 - Biblioteca Central


  5 / 131 LILACS  
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Id: biblio-1008576
Autor: Deng, Nan; Liu, Caixia; Chang, Ermei; Ji, Jing; Yao, Xiamei; Yue, Jianyun; Bartish, Igor V; Chen, Lanzhen; Jiang, Zeping; Shi, Shengqing.
Título: High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium
Fonte: Electron. j. biotechnol;25:43-49, ene. 2017. ilus, tab, graf.
Idioma: en.
Projeto: State Key Laboratory of Tree Genetics and Breeding; . 948 project; . Fund of National Non-profit Research Institutions of CAF.
Resumo: Background: Gnetum parvifolium stems and roots have been used for a long time in traditional Chinese medicines. Stilbenes are bioactive compounds present in G. parvifolium plants, and they possess antioxidative and anticancer properties. However, little is known about the responses of G. parvifolium stilbene biosynthetic pathways to stress conditions. Therefore, we investigated stilbene biosynthesis, including the expression of relevant genes, in G. parvifolium exposed to high-temperature and ultraviolet-C treatments. Results: High temperatures did not influence the accumulation of total stilbenes in stems but decreased stilbene concentrations in roots at 3 h, with a subsequent restoration to control levels. In contrast, ultraviolet irradiation induced the accumulation of total stilbenes in stems but not in roots. We also observed that high temperatures inhibited the production of resveratrol and piceatannol in G. parvifolium stems and roots, whereas ultraviolet treatments initially inhibited their accumulation (up to 6 h) but induced their production at later time points. Analyses of specific genes (i.e., PAL, C4H, 4CL, STS, and CYP) revealed that their expression levels generally increased in stress-treated stems and roots, although there was some variability in the expression profiles during treatments. Conclusions: Our results indicated that high temperatures and ultraviolet irradiation differentially affect the biosynthesis of specific stilbenes in G. parvifolium stems and roots. Therefore, cultivating G. parvifolium seedlings under optimal stress conditions may increase the biosynthesis of specific stilbene compounds.
Descritores: Estilbenos/análise
Gnetum/metabolismo
-Estilbenos/efeitos da radiação
Estilbenos/metabolismo
Raios Ultravioleta
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Gnetum/efeitos da radiação
Gnetum/genética
Plântulas
Polifenóis/análise
Polifenóis/efeitos da radiação
Polifenóis/metabolismo
CHLOROFLUOROCARBONS, METHANE1
Temperatura Alta
Responsável: CL1.1 - Biblioteca Central


  6 / 131 LILACS  
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Id: biblio-889244
Autor: Cui, Jiaqi; Bai, Li; Liu, Xiaorui; Jie, Weiguang; Cai, Baiyan.
Título: Arbuscular mycorrhizal fungal communities in the rhizosphere of a continuous cropping soybean system at the seedling stage
Fonte: Braz. j. microbiol;49(2):240-247, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Key Project of Horizontal Subject of Heilongjiang East University.
Resumo: Abstract Arbuscular mycorrhizae (AM) fungi play a crucial role in the growth of soybean; however, the planting system employed is thought to have an effect on AM fungal communities in the rhizosphere. This study was performed to explore the influence of continuous soybean cropping on the diversity of Arbuscular mycorrhizal (AM) fungi, and to identify the dominant AM fungus during the seedling stage. Three soybean cultivars were planted under two and three years continuous cropping, respectively. The diversity of AM fungi in the rhizosphere soil at the seedling stage was subsequently analyzed using polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). The results showed that an increase in cropping years improved the colonization rate of AM in all three soybean cultivars. Moreover, the dominant species were found to be Funneliformis mosseae and Glomus species. The results of cluster analysis further confirmed that the number of years of continuous cropping significantly affected the composition of rhizospheric AM fungal communities in different soybean cultivars.
Descritores: Microbiologia do Solo
Feijão de Soja/crescimento & desenvolvimento
Micorrizas/isolamento & purificação
Micorrizas/classificação
Plântulas/crescimento & desenvolvimento
Biodiversidade
Rizosfera
-Fatores de Tempo
Análise por Conglomerados
Reação em Cadeia da Polimerase
Micorrizas/genética
Eletroforese em Gel de Gradiente Desnaturante
Responsável: BR1.1 - BIREME


  7 / 131 LILACS  
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Id: biblio-889228
Autor: Ding, Chang-hong; Wang, Qian-Bo; Guo, Shenglei; Wang, Zhen-yue.
Título: The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi
Fonte: Braz. j. microbiol;49(2):362-369, Apr.-June 2018. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Heilongjiang Province.
Resumo: Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL-1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.
Descritores: Ascomicetos/metabolismo
Rumex/metabolismo
Rumex/microbiologia
Endófitos/metabolismo
Compostos Fitoquímicos/metabolismo
-Ascomicetos/isolamento & purificação
Ascomicetos/crescimento & desenvolvimento
Fatores de Tempo
Técnicas de Cocultura
Rumex/crescimento & desenvolvimento
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Plântulas/microbiologia
Endófitos/isolamento & purificação
Endófitos/crescimento & desenvolvimento
Limites: Seres Humanos
Adolescente
Responsável: BR1.1 - BIREME


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Alves, Edna Ursulino
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Id: biblio-967300
Autor: Moura, Sueli da Silva Santos; Alves, Edna Ursulino; Ursulino, Marina Matias; Bruno, Riselane de Lucena Alcântara; Anjos Neto, Antônio Pereira dos.
Título: Effect of shading on Dimorphandra gardneriana Tul. seedling production / Efeito do sombreamento na produção de mudas de Dimorphandra gardneriana Tul
Fonte: Biosci. j. (Online);34(5):1147-1157, sept./oct. 2018.
Idioma: en.
Resumo: The study of plant behavior in environments with different luminous intensities offers information about their ability to modify their growth and performance in response to light. The objective of this study was to evaluate the influence of shading on the development of Dimorphandra gardneriana Tul. Different levels of shading were used, with 0% shading (full sun) - (T1), 30% - (T2), 50% - (T3) and 70% shading - (T4). The different levels of shading were obtained by means of black polyolefin screens, sombre type, each treatment consisting of four replicates of 15 plants. The evaluated characteristics were: number of leaves, plant height, neck diameter, survival percentage, shoot length and primary root, green and dry mass of shoots and roots, total dry mass, Dickson quality index and (AP/MSPA), shoot height and diameter (A/DC), aerial part and root dry mass (MSPA/MSR), shoot height and dry mass (CPA/CR), as well as the percentage of roots (%R). The shading influenced negatively the quality of D. gardneriana seedlings, being those grown in full sun the ones that presented the best quality. Based on the morphological parameters studied, it can be affirmed that the D. gardneriana Tul., seedlings, produced in an open environment with high luminosity showed superior qualities in relation to the shaded seedlings.

O estudo do comportamento de plantas em ambientes com diferentes intensidades luminosas oferece informações sobre a capacidade que elas têm em modificar seu crescimento e desempenho em resposta à luminosidade. Assim, objetivou-se avaliar a influência do sombreamento no desenvolvimento das mudas de Dimorphandra gardneriana Tul. Foram utilizados diferentes níveis de sombreamentos, sendo estes compostos por 0% de sombreamento (pleno sol) - (T1), 30% - (T2), 50% - (T3) e 70% de sombreamento - (T4). A obtenção dos diferentes níveis de sombreamento foi por meio de telas de poliolefinas de cor preta, tipo sombrite, sendo cada tratamento composto por quatro repetições de 15 plantas. As características avaliadas foram: o número de folhas, altura da planta, diâmetro do colo, porcentagem de sobrevivência, comprimento de parte aérea e raiz primária, massa verde e seca da parte aérea e raízes, massa seca total, índice de qualidade de Dickson e a relação entre altura e diâmetro do colo (A/DC), massa seca da parte aérea e das raízes (MSPA/MSR), altura de planta e massa seca da parte aérea (AP/MSPA), comprimento da parte aérea e da raiz primária (CPA/CR), assim como a porcentagem de raízes (%R). O sombreamento influenciou negativamente a qualidade das mudas de D. gardneriana, sendo aquelas cultivadas a pleno sol as que apresentaram melhor qualidade. Com base nos parâmetros morfológicos estudados, pode-se afirmar que as mudas de D. gardneriana Tul., Produzidas em ambiente aberto com alta luminosidade, apresentaram qualidades superiores em relação às plântulas sombreadas.
Descritores: Plantas
Florestas
Raízes de Plantas
Plântulas
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-967305
Autor: Nacata, Guilherme; Andrade, Renata Aparecida de; Marques, Kelly Magalhães; Marino, Ariadne Kaleda; Mattiuz, Ben-Hur.
Título: Taquari, a new and a promising mango material: emergence, post-harvest and biometrics of fruit / Taquari: um novo e promissor material de mangueira
Fonte: Biosci. j. (Online);34(5):1178-1187, sept./oct. 2018.
Idioma: en.
Resumo: The fruit of 'Taquari' have attractive appearance, with intense yellow flesh, pleasant taste and absence of fibers, which makes it a potential material for, after a series of studies, being released as a variety. For biometrics, 50 mature fruit were used the results (average of three samples) were evaluated by descriptive statistics using central trend (average) and variability of data (standard error and coefficient of variation). For the germination test, the seeds were extracted from ripe fruit and dried; subsequently, they were germinated in sachets containing the mixture soil:sand:corral manure (3:1:1) as substrate, evaluating seedling emergence and polyembryony. The design was in randomized blocks and 5 treatments were studied, with 5 replicates each and 10 seeds per replicate. The percentage data were transformed into arc-sen, submitted to analysis of variance and the means, to Tukey test at 5% probability. For fruit quality characterization, 12 ripe fruits were used, separated into 3 lots with 4 fruit, using a completely randomized design. The fruit were characterized regarding firmness, peel and pulp color, soluble solids, titratable acidity, pH, ascorbic acid and respiratory rate. We conclude that the 'Taquari' has fruit with interesting characteristics for the Brazilian market and there is a better seedling emergence in the absence of seed coat, but with a greater occurrence of polyembryony. For quality characterization, the fruit present parameters such as soluble solids and titratable acidity similar to the main varieties of mangoes sold in the country, but with emphasis to the high ascorbic acid content detected.

Os frutos da mangueira 'Taquari' têm aparência atrativa, com polpa amarelo intenso, sabor agradável e ausência de fibras, o que a torna um material com grande potencial para futuramente ser lançado como variedade. Com o objetivo de avaliar caracteres fenotípicos relativos emergência, biometria e pós-colheita, do material 'Taquari' este trabalho foi desenvolvido. Para a biometria foram utilizados 50 frutos maduros, os resultados foram avaliados por estatística descritiva, utilizando-se de medida de tendência central (média) e de variabilidade de dados (desvio-padrão). Para o teste de germinação, as sementes foram extraídas de frutos maduros e colocadas para secar, em seguida, foram colocadas para germinar em saquinhos contendo como substrato mistura de solo:areia:esterco de curral curtido (3:1:1), avaliando-se a emergência das plântulas e a poliembrionia. O delineamento foi em blocos casualizados e foram estudados 5 tratamentos, com 5 repetições cada e 10 sementes por repetição. Os dados de porcentagem foram transformados em arc-sen, submetidos à análise de variância e as médias ao teste Tukey a 5% de probabilidade. Para a caracterização de qualidade dos frutos, utilizaram-se 12 frutos maduros, separadas em 3 lotes com 4 frutos, utilizando delineamento inteiramente casualizado. Os frutos foram caracterizados quanto a firmeza, coloração de casca e polpa, sólidos solúveis, acidez titulável, pH, ácido ascórbico e taxa respiratória. Conclui-se que a mangueira 'Taquari' apresenta frutos com características interessantes para o mercado brasileiro e há melhor emergência das plântulas na ausência do tegumento da semente, porém com maior ocorrência de poliembrionia. Para a caracterização de qualidade, os frutos apresentam parâmetros como de sólidos solúveis e acidez titulável similares às principais cultivares de mangas comercializadas no país, porém com destaque para os altos teores de ácido ascórbico encontrados.
Descritores: Sementes
Solo
Germinação
Mangifera
Plântulas
Esterco
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-968898
Autor: Oliveira, Annie Carolina Araújo de; Machado, Caroline de Araújo; Oliveira, Leila Albuquerque Resende de; Padilha, Francine Ferreira; Silva, Ana Veruska Cruz da; Lédo, Ana da Silva.
Título: Induction and growth curve of calli from leaf and nodal explants of genipap / Indução e curva de crescimento de calos obtidos de explantes foliares e nodais de jenipapeiro
Fonte: Biosci. j. (Online);34(6 Supplement 1):161-167, nov./dec. 2018.
Idioma: en.
Resumo: The aim of this study was to determine the effect of the auxin 2,4-D (2,4-dichlorophenoxyacetic) in calli formation from leaf and nodal segments of genipap and to characterize its growth curve. Explants obtained from shoots previously established from in vitro seedlings were used for calli induction. The experimental design was completely randomized in a 3x5x2 factorial with three accessions (NB, SA, SAL), five concentrations of 2,4-D (0.0; 2.0; 4.0, 6.0 or 8.0 mg L-1) and two times of measurement for calli fresh weight (30 and 60 days). There was callus formation in all treatments tested. It was observed that the best response for callus induction from leaf segments was with 2.0 mg L-1 of 2,4-D. For the nodal segment, the response among the accessions was different due to 2,4-D concentrations. The growth curve was plotted according to the fresh weight of callus obtained at intervals of 10 days up to 60 days. Through the established growth curve, the nodal-derived calli from accession SA should be transferred to a new medium, after 40 days of culture.

O objetivo desse trabalho foi determinar o efeito da auxina 2,4-D (ácido diclorofenoxiacético) na calogênese de segmentos foliar e nodal de jenipapeiro e caracterizar sua curva de crescimento. Explantes obtidos de brotações pré-estabelecidas a partir de plântulas in vitro foram utilizados na indução de calos. O delineamento experimental utilizado foi o inteiramente casualizado em esquema fatorial 3x5x2, com três acessos (NB, SA e SAL), cinco concentrações de 2,4-D (0,0; 2,0; 4,0; 6,0 ou 8,0 mg L-1) e dois tempos de avaliação (30 e 60 dias) da massa fresca de calos. Houve formação de calos em todos os tratamentos testados. Observou-se que a melhor resposta de indução ocorreu na concentração de 2,0 mg L-1 para calos oriundos de segmentos foliares. Para o segmento nodal a resposta entre os acessos foi diferenciada em função das concentrações de 2,4-D. A curva de crescimento foi plotada a partir da massa fresca dos calos obtida em intervalos de 10 dias até os 60 dias. Através da curva de crescimento estabelecida, os calos derivados de segmentos nodais do acesso SA devem ser transferidos para um novo meio de cultura, 40 dias após à inoculação.
Descritores: Rubiaceae
Plântulas
Gráficos de Crescimento
Responsável: BR396.1 - Biblioteca Central



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