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Pesquisa : A21.249 [Categoria DeCS]
Referências encontradas : 15 [refinar]
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Id: biblio-974345
Autor: Wang, Chuan-Xu; Li, Xin.
Título: JMT-1: a novel, spherical lytic halotolerant phage isolated from Yuncheng saline lake
Fonte: Braz. j. microbiol;49(supl.1):262-268, 2018. graf.
Idioma: en.
Projeto: Key Disciplines Construction Foundation of the \"1331 Project\" of Shanxi Province; . Natural Science Foundation of Shanxi Province; . PhD Start-up Foundation of Yuncheng University.
Resumo: ABSTRACT This work described a novel halotolerant phage, JMT-1, with a spherical morphology. JMT-1, which was isolated from a hypersaline lake, could produce clear plaques on Chromohalobacter sp. LY7-3. The purified virions are spherical, have no visible tail, and are about 30-50 nm in diameter. JMT-1 has a wide host range, and this study showed that the phage can infect at least five halophilic bacteria. The proteins of JMT-1 were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and six proteins were detected. Results show that JMT-1 is a bacteriophage with a linear double-stranded DNA. Meanwhile, the genome is approximately 23 kb in length and is sensitive to the restriction endonucleases Bam I, EcoR I, Hind III and Kpa I. JMT-1 has a high titer, approaching 1.5 × 109 pfu/mL after dilution to 10−6 pfu/mL. The phage is also sensitive to chloroform but not to temperature, pH, and lowered salt concentration. JMT-1 is a spherical lytic halotolerant phage with a wide host range and has the tolerance to specific extreme environments. These data could provide references for studying phage resources in extreme environments and would also provide the useful methods for isolation and identification of other valuable phage in the salt lake environment.
Descritores: Bacteriófagos/isolamento & purificação
Vírion/isolamento & purificação
Lagos/virologia
Especificidade de Hospedeiro
-Bactérias/virologia
Bacteriófagos/classificação
Bacteriófagos/fisiologia
Bacteriófagos/genética
Vírion/classificação
Vírion/fisiologia
Cloreto de Sódio/análise
Lagos/análise
China
Genoma Viral
Responsável: BR1.1 - BIREME


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Id: biblio-901502
Autor: García Ariza, Leidy Lorena; Olaya Montes Quim, Jorge Humberto; Sierra Acevedo, Jorge Iván; Padilla Sanabria, Leonardo.
Título: Actividad biológica de tres Curcuminoides de Curcuma longa L. (Cúrcuma) cultivada en el Quindío-Colombia / Biological activity of three curcuminoids from Curcuma longa L. (turmeric) grown in Quindío, Colombia
Fonte: Rev. cuba. plantas med;22(1):0-0, ene.-mar. 2017. ilus, tab.
Idioma: es.
Resumo: Introducción: Curcuma longa L. es una planta de la familia Zingiberaceae distribuida en las regiones tropicales y subtropicales, utilizada en la industria alimentaria, en medicina y en cosmética. Su colorante principal es la curcumina, un polifenol con múltiples efectos medicinales. Objetivos: obtener, caracterizar químicamente y evaluar la actividad biológica de tres curcuminoides de C. longa, cultivada en el Quindío-Colombia. Métodos: se purificaron tres curcuminoides (curcumina (C), demetoxicurcumina (DMC) y bisdemetoxicurcumina (BDMC) desde el rizoma de la planta, en estado seco, por cromatografía en columna y se caracterizaron por punto de fusión, espectroscopía infrarroja (IR), espectroscopía UV-vis y espectrometría de masas. Se evaluó la actividad antimicrobiana en bacterias y hongos por el método modificado de pozos de agar, la citotoxicidad sobre células BHK-21 por el método de bromuro de 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol (MTT) y la toxicidad sobre Artemia salina. Finalmente se determinó el efecto de los curcuminoides en células BHK-21 infectadas con dengue virus 2. Resultados: la curcumina presentó mayor punto de fusión (177,3 ºC-183,2 ºC). El espectro IR reveló los grupos funcionales característicos y el UV-vis indicó máximos de absorción para curcumina, demetoxicurcumina y bisdemetoxicurcumina de 419, 418 y 414 nm en cloroformo, respectivamente. El espectro de masas mostró m/z para C: 368, DMC: 338 y BDMC: 308. Se encontró actividad antimicrobiana frente a Staphylococcus aureus y Staphylococcus epidermidis, se determinó que BDMC presentó menor toxicidad y se evidenció mayor efecto inhibitorio sobre viriones infectivos de dengue con curcumina a 20 y 30 M. Conclusiones: la caracterización de los compuestos confirma su composición como polifenoles, lo cual se relaciona a la actividad biológica de éstos, encontrándose principalmente que la curcumina altera la infección por virus dengue en cultivo celular. Esta investigación confirma la importancia de los principios activos de plantas con amplio espectro farmacológico como C. longa(AU)

Introduction: Curcuma longa L. is a plant from the family Zingiberaceae distributed in tropical and subtropical regions and used in the food industry, in medicine and in cosmetics. Its main coloring substance is curcumin, a polyphenol with many medicinal properties. Objectives: Obtain, characterize chemically and evaluate the biological activity of three curcuminoids from C. longa grown in Quindío, Colombia. Methods: Three curcuminoids (curcumin (C), demethoxycurcumin (DMC) and bisdemethoxycurcumin BDMC) from the rhizome of the plant were purified in a dry state by column chromatography and characterized by fusion point, infrared (IR) spectroscopy, UV-vis spectroscopy and mass spectrometry. Antimicrobial activity against bacteria and fungi was evaluated by the modified agar well method, cytotoxicity to BHK-21 cells by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and toxicity against Artemia salina. Finally, determination was made of the effect of the curcuminoids in BHK-21 cells infected with dengue virus 2. Results: Curcumin had the highest fusion point (177.3 ºC-183.2 ºC). IR spectroscopy revealed the characteristic functional groups and UV-vis spectroscopy showed maximum absorption values for curcumin, demethoxycurcumin and bisdemethoxycurcumin of 419, 418 and 414 nm in chloroform, respectively. Mass spectrometry found that m/z values were C: 368, DMC: 338 and BDMC: 308. Antimicrobial activity was observed against Staphylococcus aureus and Staphylococcus epidermidis. BDMC was found to have lower toxicity. A greater inhibitory effect against infective dengue virions was observed with curcumin at 20 y 30 µM. Conclusions: Characterization of the compounds confirms their polyphenolic composition, which manifests in their biological activity, mainly the capacity of curcumin to alter infection by dengue virus in cell cultures. The study corroborated the importance of the active principles of plants with a wide pharmacological spectrum, such as C. longa(AU)
Descritores: Curcuma/efeitos dos fármacos
-Vírion
Cromatografia em Camada Delgada/métodos
Colômbia
Produtos com Ação Antimicrobiana
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-788996
Autor: Barreto-Vieira, Debora Ferreira; Barth, Ortrud Monika; Silva, Marcos Alexandre Nunes da; Santos, Carolina Cardoso; Santos, Aline da Silva; F Filho, Joaquim Batista; Filippis, Ana Maria Bispo de.
Título: Ultrastructure of Zika virus particles in cell cultures
Fonte: Mem. Inst. Oswaldo Cruz;111(8):532-534, Aug. 2016. graf.
Idioma: en.
Projeto: CNPq.
Resumo: Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.
Descritores: Vírion/ultraestrutura
Zika virus/ultraestrutura
-Técnicas de Cultura de Células
Chlorocebus aethiops
Genoma Viral
Microscopia Eletrônica de Transmissão
Reação em Cadeia da Polimerase em Tempo Real
Células Vero
Replicação Viral
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Roehe, Paulo Michel
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Id: lil-748263
Autor: Kruger, Ernesto Renato; Penha, Tania Regina; Stoffelo, Daura Regina Eira; Roehe, Paulo Michel; Ribeiro, Magda Costa; Soccol, Vanete Thomaz.
Título: Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Fonte: Braz. j. microbiol;46(1):279-283, 05/2015. graf.
Idioma: en.
Resumo: Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
Descritores: Doenças dos Bovinos/virologia
Infecções por Herpesviridae/veterinária
/classificação
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/classificação
/isolamento & purificação
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/isolamento & purificação
Infecções Tumorais por Vírus/veterinária
-Brasil
Efeito Citopatogênico Viral
DNA Viral/genética
DNA Viral/metabolismo
Exsudatos e Transudatos/virologia
Infecções por Herpesviridae/virologia
/genética
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/genética
Microscopia Eletrônica de Transmissão
Polimorfismo de Fragmento de Restrição
Infecções Tumorais por Vírus/virologia
Útero/patologia
Útero/virologia
Cultura de Vírus
Vírion/ultraestrutura
Limites: Animais
Bovinos
Feminino
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-723135
Autor: Souza, Marcos Michel de; Manzine, Livia Regina; Silva, Marcos Vinicius G. da; Bettini, Jefferson; Portugal, Rodrigo Vilares; Cruz, Angela Kaysel; Arruda, Eurico; Thiemann, Otavio Henrique.
Título: An improved purification procedure for Leishmania RNA virus (LRV)
Fonte: Braz. j. microbiol;45(2):695-698, Apr.-June 2014. ilus.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); . CAPES; . FAPESP; . CNPq.
Resumo: Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
Descritores: Leishmaniavirus/isolamento & purificação
Vírion/isolamento & purificação
Virologia/métodos
-Leishmaniavirus/ultraestrutura
Microscopia Eletrônica de Transmissão
Coloração e Rotulagem/métodos
Vírion/ultraestrutura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-696137
Autor: Universidad Nacional de ColombiaMoreno, Luz Yurany(edt); Universidad Nacional de Colombia(edt); Universidad Nacional de Colombia(edt).
Título: Expresión y purificación de las proteínas estructurales del rotavirus VP5* y VP8* en bacterias E. coli BL21(DE3) / Expression and purification of rotavirus structural proteins VP5* and VP8* in bacteria E. coli BL21(DE3)
Fonte: Rev. colomb. biotecnol;15(1):82-97, ene.-jun. 2013. ilus.
Idioma: es.
Resumo: La caracterización de las proteínas estructurales del rotavirus y de las proteínas de la superficie de la célula hospedera implicadas en la unión y penetración del virion requiere de la disponibilidad de cantidades suficientes y de alto grado de pureza de estas proteínas. Por lo tanto, el objetivo de este trabajo fue expresar y purificar las proteínas estructurales del rotavirus de la cepa RRV, VP5* y VP8*, y producir anticuerpos policlonales dirigidos contra ellas. Se expresaron las proteínas recombinantes VP5* (rVP5*) y VP8* (rVP8*) en bacterias E. coli BL21(DE3) transfectadas con el plásmido pGEX-4T que contenía sus secuencias codificantes. Se consideraron como variables el medio de crecimiento, número de bacterias antes de inducir la expresión, concentración del inductor y tiempo de inducción. La mayor proporción de rVP8* se obtuvo cuando las bacterias transformadas se cultivaron en medio LB y la inducción se llevó a cabo con 1 mM de IPTG cuando el cultivo alcanzó una OD 600 nm de 0.5 y la inducción se mantuvo durante 6 h. rVP5* alcanzó la mayor proporción cuando células a una OD 600 nm de 0.2 fueron inducidas con 0.5 mM de IPTG durante 4 h en medio 2XYT en presencia de glucosa al 2 %. Las proteínas recombinantes obtenidas, acumuladas en la fracción insoluble, fueron solubilizadas con detergentes iónicos y no iónicos, seguido de purificación mediante cromatografía de afinidad antes de ser empleadas como antígenos para la producción de anticuerpos policlonales en conejos. Estos anticuerpos se caracterizaron mediante su capacidad de reconocimiento de los antígenos correspondientes en ELISA, "Western blotting", y en ensayos de inmunocitoquímica en células infectadas con rotavirus RRV. La cantidad y el grado de pureza de las proteínas recombinantes obtenidas, y los anticuerpos dirigidos contra ellas, anticipan su utilidad como herramientas en la caracterización de la interacción virus-célula.

The characterization of rotavirus structural proteins and the cell surface proteins involved in virion binding and penetration depends on the availability of substantial amounts of highly purified proteins. The aim of the present work was to express and purify the rotavirus structural proteins VP5* and VP8* in order to produce polyclonal antibodies against them. Recombinant proteins VP5* (rVP5*) and VP8* (rVP8*) were expressed in E. coli cells transfected with pGEX-4T or pET 28a containing their corresponding encoding sequences. Culture medium, bacterial concentration before induction, inductor concentration and induction time were used as variables. The greater proportion of rVP8* was obtained when transfected bacteria were grown in medium LB and induction was started by adding 1 mM IPTG to cells at OD 600 nm 0.5 followed by 6 h-induction. The highest proportion of rVP5* was reached when cells at OD 600 nm 0.2 were induced with 0.5 mM IPTG for 4 h in medium 2XYT containing 2% glucose. The recombinant proteins accumulated in the insoluble fraction were solubilized with ionic and non-ionic detergents, and purified by affinity chromatography before being used as antigens for production of rabbit polyclonal antibodies. The antibodies produced were characterized through their ability to recognize the corresponding antigens in ELISA, Western blotting, and immunochemistry assays in rotavirus infected cells. The amount and purity of recombinant proteins obtained in this work, and the antibodies against them, are expected to be useful for the characterization of the virus-cell interaction.
Descritores: Escherichia coli
Proteínas
Rotavirus
-Anticorpos
Vírion
Responsável: CO326 - Departamento de Biología


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Id: lil-634616
Autor: Stella, E. J.; De La Iglesia, A. I.; Morbidoni, H. R..
Título: Mycobacteriophages as versatile tools for genetic manipulation of mycobacteria and development of simple methods for diagnosis of mycobacterial diseases / Micobacteriófagos como herramientas versátiles para la manipulación genética y el desarrollo de métodos simples para el diagnóstico de enfermedades micobacterianas
Fonte: Rev. argent. microbiol;41(1):45-55, ene.-mar. 2009. ilus.
Idioma: en.
Resumo: Tuberculosis, caused by Mycobacterium tuberculosis, is responsible for over two million deaths per year worldwide. Due to its long doubling time (18 h), the microbiological detection of M. tuberculosis by conventional methods takes up to one month, unless the number of bacilli in the biological sample is high enough. Thus, drug resistance assessment requires at least one month for obtaining the primary culture and another month to determine its susceptibility to antimycobacterial drugs. Moreover, for a long time, the lack of genetic tools for mycobacteria has been a barrier for undertaking studies aimed at understanding the mechanisms of drug resistance and drug target identification, being all these topics of utmost importance considering the increase in the number of drug-resistant clones and the few therapeutic options available. Mycobacteriophages are promising as a novel source of genetic elements for mycobacteria manipulation, as well as for the development of versatile, simple, fast and cheap methods for drug resistance assessment of M. tuberculosis clinical isolates. We herein describe the background related to the use of mycobacteriophages, with emphasis placed on their utilization for drug resistance analysis in our country.

La tuberculosis, enfermedad causada por el bacilo Mycobacterium tuberculosis, es responsable de más de dos millones de muertes anuales en el mundo. Debido a su largo tiempo de duplicación (18 h), la detección bacteriológica de M. tuberculosis por métodos convencionales necesita de un mes o aun más, a menos que el número de bacilos en la muestra clínica sea suficientemente alto. Por consiguiente, se necesita un mínimo de dos meses para determinar la resistencia de este microorganismo a las drogas antituberculosas: uno para obtener el cultivo primario y otro para ensayar la sensibilidad frente a aquellas. La falta de herramientas para la manipulación genética de micobacterias ha dificultado la identificación de los blancos de acción de las drogas y el estudio de los mecanismos de resistencia a éstas, tópicos de la mayor relevancia dado el aumento mundial del número de aislamientos clínicos multirresistentes y las pocas opciones terapéuticas disponibles. Los micobacteriófagos son considerados nuevas herramientas para la manipulación de las micobacterias, así como para el desarrollo de métodos simples, rápidos y económicos para determinar la sensibilidad a drogas de los aislamientos clínicos de M. tuberculosis. En esta revisión se describen los antecedentes del uso de micobacteriófagos con énfasis en su utilización para el análisis de resistencia a drogas antituberculosas en nuestro país.
Descritores: Tipagem de Bacteriófagos/métodos
Micobacteriófagos/genética
Mycobacterium tuberculosis/genética
Transdução Genética
Tuberculose/diagnóstico
-Líquidos Corporais/microbiologia
América Latina
Microscopia Eletrônica
Testes de Sensibilidade Microbiana/métodos
Micobacteriófagos/isolamento & purificação
Micobacteriófagos/ultraestrutura
Mycobacterium tuberculosis/virologia
Reação em Cadeia da Polimerase
Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Tuberculose/microbiologia
Vírion/ultraestrutura
Limites: Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Revisão
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-537208
Autor: Ortega, Horacio; Martín-Landrove, Miguel.
Título: An epitope variable-reservoir model for HIV-1 infection
Fonte: Acta cient. venez;55(3):247-263, 2004. graf.
Idioma: en.
Resumo: Se presenta un modelo de infección por VIH-1 basado en el concepto de inmunodominancia. Además de viriones mutantes y células T-CD4, se considera también reservorios virales, entre ellos macrófagos y células dendríticas foliculares. También se considera ataque citotóxico contra los reservorios y ataque extracelular sobre los viriones, ambos coordinados por las células T-CD4. En primer lugar, se trabaja sólo con una variable viral, y se usan ciertas aproximaciones para obtener un modelo fácilmente manipulable, para el cual se encuentra un criterio de estabilidad que rige el paso de progresión a regresión de la infección. Este criterio mantiene su validez para el modelo sin uso de aproximaciones, esto es, dos epítopes que mutan al azar, cada uno de ellos con dos variantes. Los resultados sugieren: a) que el papel jugado por los reservorios en el mantenimiento de la infección es muy importante, b) que mantener el ataque de las células citotóxicas sobre los reservorios infectados facilita la labor del sistema inmune, y c) que la terapia debería orientarse preferiblemente hacia estos objetivos.
Descritores: Genes MHC da Classe II
HIV-1
Sistema Imunitário
Reservatórios de Água
Vírion
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Texto completo SciELO Brasil
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Id: lil-528168
Autor: Pereira, G. C; Granato, A; Figueiredo, A. R; Ebecken, N. F. F.
Título: Virioplankton abundance in trophic gradients of an upwelling field
Fonte: Braz. j. microbiol;40(4):857-865, Oct.-Dec. 2009. graf, mapas, tab.
Idioma: en.
Resumo: This work correlates time series of biological and physical variables to the marine viruses across trophic gradients within Arraial do Cabo upwelling system, Southeast of Brazil. The objective is to investigate the major controlling factors of virioplankton dynamics among different water masses. It was used an in situ and ex situ flow cytometry for accessing the plankton community. Viruses were highly correlated to bacteria and phytoplankton, but although the lack of direct correlation with physicals, upwelling turned out to be the main contributing factor to the highest values of viral abundance and virus:bacterial ratio. Our data suggest that the lowest temperature of upwelled South Atlantic Central Waters would help to maintain a high viral abundance and higher temperatures of Coastal and Tropical Waters might be another ecological niche allowing the co-existence.
Descritores: Flora Aquática
Citometria de Fluxo
Fitoplâncton/genética
Gradiente
Técnicas In Vitro
Plâncton/isolamento & purificação
Vírion/genética
-Métodos
Técnicas
Correntes de Água
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-449531
Autor: Pujol, Flor.
Título: Biología de los virus de hepatitis / Biology of the hepatitis viruses
Fonte: Acta cient. Soc. Venez. Bioanalistas Esp;6(1/2):5-12, 2000. graf.
Idioma: es.
Resumo: El alfabeto que conforman los virus causantes de hepatitis ha crecido en forma significativa en estos últimos años. Estos virus pertenecen a familias virales muy distintas y se dividen además en virus de transmisión entérica y transmisión parenteral. Antes de 1989, se conocía el VHA, picornavirus de transmisión entérica y sin mayores secuelas, el VHB, hepatitis de transmisión parenteral, con secuelas de cirrocis y cáncer de hígado y el VHD, daltavirus que requiere una co-infección por el VHB para poder replicarse en forma efectiva y responsable de muchos casos de hepatitis fulminantes. En 1989, se descubre por técnicas de biología molecular, el VHC, hepacivirus miembro de la familia flaviviridae, de transmisión parenteral cuya infección está asociada a secuelas similares a las del VHB. En 1990 se identifica el VHE, virus de transmisión entérica. El VHF es un término reservado a un virus de transmisión entérica, cuya identificación en la India es controversial. En la búsqueda del virus reponsable de la hepatitis post-transfusional no A hasta no E, entre 1995 y 1996 se describe el denominado VHG, de transmisión parenteral, utilizando herramientas cada vez más novedosas de biología molecular, aunque este virus no parece causar hepatitis. Mas recientemente se identifica el TTV(1997) y el Sen-V(2000), de nuevo por técnicas de biología molecular ¿Será algunos de estos virus al fin el responsable de las hepatitis post-transfusionales no A hasta no G?
Descritores: Epidemiologia
Genoma
Vírus de Hepatite
Hepatite C/transmissão
Hepatite E/transmissão
Vírion
Virologia
-Biologia Molecular
Venezuela
Limites: Masculino
Humanos
Feminino
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha



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