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Id: biblio-950505
Autor: Santos, Luana dos; Campos, Adriana; Cechinel Filho, Valdir; Nesello, Luciane Angela Nottar.
Título: Perfil fitoquímico e atividade gastroprotetora dos frutos de Eugenia mattosii / Phytochemical profile and gastroprotective activity of eugenia mattosii fruits
Fonte: Arq. gastroenterol;55(2):138-141, Apr.-June 2018. tab.
Idioma: en.
Resumo: ABSTRACT BACKGROUND: Extracts obtained from plants and fruits provide a relatively safe and practical alternative for the conventional medicine of gastrointestinal diseases. The specie Eugenia mattosii, popularly known in Brazil as "cerejinha", belongs to Myrtaceae family. Species of this family present pharmacological properties, and can be used in the treatment of gastrointestinal disorders. OBJECTIVE: The aim of this study was to determine the phytochemical profile and evaluate the gastroprotective activity of Eugenia mattosii fruits. METHODS: Phytochemical analysis was carried out by thin layer chromatography and gastroprotective assays were performed using two experimental models: acute ulcer model induced by ethanol/HCl and acute ulcer model induced by non-steroidal anti-inflammatory drug (indomethacin). Total lesion area (mm2) and relative lesion area (%) were determined. RESULTS: The results of the phytochemical analysis indicated that the bark and pulp and seeds of E. mattosii present phenolic compounds, terpenes and/or steroids. In gastric ulcer model induced by ethanol was evidenced significant reduction of damaged areas for doses of 50 and 250 mg/ kg of seeds methanol extract, while in the indomethacin-induced ulcer model, all parts of the fruit presented defense capability of the gastric mucosa by reducing lesions at doses of 50, 125 and 250 mg/kg. CONCLUSION: The results demonstrate that the specie E. mattosii has bioactive compounds that provide gastroprotective activity, presenting possible therapeutic potential.

RESUMO CONTEXTO: Extratos obtidos de plantas e frutos fornecem uma alternativa relativamente segura e prática para os remédios convencionais de doenças gastrointestinais. A espécie Eugenia mattosii, popularmente conhecida no Brasil como "cerejinha", pertence à família Myrtaceae. Espécies desta família apresentam propriedades farmacológicas e podem ser utilizadas no tratamento de distúrbios gastrointestinais. OBJETIVO: O objetivo deste estudo foi determinar o perfil fitoquímico e avaliar a atividade gastroprotetora dos frutos de Eugenia mattosii. MÉTODOS: A análise fitoquímica foi realizada por cromatografia em camada delgada e dois modelos experimentais foram utilizados para avaliação da atividade gastroprotetora em camundongos: modelo de úlcera gástrica induzida por anti-inflamatório não-esteroidal (indometacina) e modelo de úlcera gástrica induzida por etanol/HCl. RESULTADOS: Os resultados da análise fitoquímica indicaram que a casca e polpa e as sementes de E. mattosii apresentam compostos fenólicos, terpenos e/ou esteroides. No modelo de úlcera gástrica induzido pelo etanol, foi evidenciada redução significativa de áreas danificadas para doses de 50 e 250 mg/kg do extrato das sementes, enquanto no modelo de úlcera induzida por indometacina, todas as partes do fruto apresentaram capacidade de defesa da mucosa gástrica ao reduzir as lesões nas doses de 50, 125 e 250 mg/kg. CONCLUSÃO: Os resultados demonstram que a espécie E. mattosii possui compostos bioativos com atividade gastroprotetora, apresentando possível potencial terapêutico.
Descritores: Úlcera Gástrica/prevenção & controle
Extratos Vegetais/farmacologia
Substâncias Protetoras/farmacologia
Eugenia/química
Frutas/química
Mucosa Gástrica/efeitos dos fármacos
Antiulcerosos/farmacologia
-Sementes/química
Úlcera Gástrica/induzido quimicamente
Brasil
Extratos Vegetais/administração & dosagem
Indometacina
Modelos Animais de Doenças
Etanol
Compostos Fitoquímicos/farmacologia
Fitoterapia
Antiulcerosos/administração & dosagem
Antioxidantes/análise
Antioxidantes/farmacologia
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1287237
Autor: Sadava, Emmanuel E; Campos Arbulú, Ana L; Maya, Manuel E; Mezzadri, Norberto A.
Título: Comportamiento biológico de mallas para la reparación de paredes abdominales. Resultados preliminares en un modelo animal / Biological behavior of meshes used for abdominal wall surgery. Preliminary results in an animal model
Fonte: Medicina (B.Aires);81(1):24-30, mar. 2021. graf.
Idioma: es.
Resumo: Resumen La falla en la reparación de los defectos de la pared abdominal se relaciona con una alteración en la integración del material protésico. El objetivo de este trabajo fue evaluar el comportamiento biológico de mallas utilizadas en cirugía de paredes abdominales en un modelo animal. Luego de la confección de un defecto parietal se colocó una malla intraperitoneal, utilizando 4 grupos de 10 ratas; 1) Prolene (polipropileno microporo de alto peso), 2) Ultrapro (polipropileno + poliglecaprone, macroporo de bajo peso), 3) Proceed (polipropileno + polidoxanona + celulosa oxidada regenerada, macroporo de peso intermedio), y 4) Physiomesh (polipropileno + poliglecaprone, macroporo de bajo peso). Se realizó análisis macroscópico y microscópico a los 30 días y los resultados fueron evaluados por dos observadores independientes. Al examen macroscópico, la integración de la prótesis fue > 75% en todos los grupos. El análisis microscópico mostró mayor inflamación global y número de células gigantes multinucleadas en Prolene (p < 0.01) y menor cantidad de células inflamatorias en la interface músculo-malla en Physiomesh < Ultrapro (p < 0.05). La organización de las fibras de colágeno fue similar para todas las mallas, aunque hubo mayor depósito de colágeno en los espacios inter-filamento para las mallas macroporosas (p < 0.01). Concluimos que las mallas de polipropileno microporo y alto peso producen mayor reacción inflamatoria y de cuerpo extraño. Por lo tanto, las mallas compuestas tendrían una mejor biocompatibilidad y serían mejor toleradas por el huésped.

Abstract An adequate integration of the prosthetic materials used to repair abdominal wall defects is necessary for satisfactory outcomes. We aimed to evaluate, in an animal model, the biological behavior of meshes used for abdominal wall surgery. Four groups of 10 rats were separated. After laparotomy, intraperitoneal prostheses were placed: 1) Prolene (polypropylene microporous, heavy-weight), 2) Ultrapro (polypropylene + poliglecaprone, macroporous low-weight), 3) Proceed (polypropylene + polidoxanone + regenerated oxidized cellulose, microporous medium-weight), 4) Physiomesh (polypropylene + poliglecaprone, macroporous lowweight). Macroscopic and microscopic analyses were performed at 30 days. The results were evaluated by two independent observers and expressed in means with standard deviation. For statistical analysis p < 0.05 was considered significant. On macroscopic examination, mesh integration was greater than 75% in all cases. Microscopic analysis showed greater global inflammation and more multinucleated giant cells in Prolene (p < 0.01). Less inflammatory cells were observed at the muscle-mesh interface in Physiomesh vs. Ultrapro (p < 0.05). Collagen fibers disposition was similar in all meshes, although, microporous meshes had higher collagen deposit in the interfilamentous spaces (p < 0.01). In conclusion, in our animal model, microporous and heavy-weight polypropylene meshes produce greater inflammatory and foreign body reaction. Thus, composite meshes would have greater biocompatibility and better tolerance by the host.
Descritores: Telas Cirúrgicas/efeitos adversos
Parede Abdominal/cirurgia
-Poliésteres
Próteses e Implantes
Teste de Materiais
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-976025
Autor: Silva, Flavia Rodrigues da; Paiva, Mayara Rodrigues Brandão de; Dourado, Lays Fernanda Nunes; Silva, Rummenigge Oliveira; Silva, Carolina Nunes da; Costa, Bruna Lopes da; Toledo, Cibele Rodrigues; Lima, Maria Elena de; Silva-Cunha, Armando da.
Título: Intravitreal injection of the synthetic peptide LyeTx I b, derived from a spider toxin, into the rabbit eye is safe and prevents neovascularization in a chorioallantoic membrane model
Fonte: J. venom. anim. toxins incl. trop. dis;24:31, 2018. graf, ilus.
Idioma: en.
Projeto: São Paulo Research Foundation (FAPESP, Brazil); . \"Conselho Nacional de Desenvolvimento Científico e Tecnológico\" (CNPq, Brazil); . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); . CAPES; . CNPq/CAPES.
Resumo: The great diversity of molecules found in spider venoms include amino acids, polyamines, proteins and peptides, among others. Some of these compounds can interact with different neuronal receptors and ion channels including those present in the ocular system. To study potential toxicity and safety of intravitreal injection in rabbits of LyeTx I b, a synthetic peptide derived from the toxin LyeTx I found in venom from the spider Lycosa eritrognatha and to evaluate the angiogenic activity on a CAM model. Methods: ARPE-19 cells were treated with LyeTx I b (0.36; 0.54; 0.72; 2.89; 4.34 or 9.06 µM). In this study, New Zealand rabbits were used. LyeTx I b (2.89 µM) labeled with FITC dissolved in PBS, or only PBS, were injected into vitreous humor. Electroretinogram (ERG) was recorded 1 day before injection and at 7,14 and 28 days post-injection. Clinical examination of the retina was conducted through tonometer and eye fundus after ERG. Eyes were enucleated and retinas were prepared for histology in order to assess retinal structure. CAMs were exposed to LyeTx I b (0.54; 0.72; 2.17 or 2.89 µM). Results: ARPE-19 cells exposed to LyeTx I b showed cell viability at the same levels of the control. The fluorescence of LyeTx I b labeled with FITC indicated its retinal localization. Our findings indicate ERG responses from rats injected in the eye with LyeTx I b were very similar to the corresponding responses of those animals injected only with vehicle. Clinical examination found no alterations of intraocular pressure or retinal integrity. No histological damage in retinal layers was observed. CAM presented reduced neovascularization when exposed to LyeTx I b. Conclusions: Intravitreal injection of LyeTx I b is safe for use in the rabbit eye and prevents neovascularization in the CAM model, at Bevacizumab levels. These findings support intravitreal LyeTx l b as a good candidate to develop future alternative treatment for the retina in neovascularization diseases.(AU)
Descritores: Peptídeos
Neovascularização de Coroide
Injeções Intravítreas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-976031
Autor: Cordeiro, Francielle Almeida; Coutinho, Bárbara Marques; Wiezel, Gisele Adriano; Bordon, Karla de Castro Figueiredo; Bregge-Silva, Cristiane; Rosa-Garzon, Nathalia Gonsales; Cabral, Hamilton; Ueberheide, Beatrix; Arantes, Eliane Candiani.
Título: Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Fonte: J. venom. anim. toxins incl. trop. dis;24:32, 2018. tab, graf.
Idioma: en.
Projeto: Coordination for the Improvement of higher Education Personnel (CAPES); . National Institute of Health (NIH); . São Paulo Research Foundation (FAPESP); . CAPES; . CNPq/CAPES.
Resumo: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV). Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed proteolytic activity on azocasein with maximum activity at pH 7.0-9.0. It was inhibited by EDTA (a metal chelator that removes zinc, which is essential for enzymatic activity) and no effect was observed with PMSF, iodoacetic acid or pepstatin (inhibitors of serine, cysteine and aspartyl proteases, respectively). Ca2+, Mg2+ and Ba2+ ions increased its activity, while Al3+, Cu2+, Ni2+ and Zn2+ inhibited it. Additionally, ZnCl2 showed a dose dependent inhibition of the enzyme. Lmr-MP activity was also evaluated upon chromogenic substrates for plasma kallikrein (S-2302), plasmin and streptokinase-activated plasminogen (S-2251) and Factor Xa (S-2222) showing the highest activity on S-2302. The activity in different solutions (5 mM or 50 mM ammonium bicarbonate, pH 7.8; 0.1% trifluoroacetic acid + 50% acetonitrile; phosphate buffer saline, pH 7.4; 50 mM sodium acetate, pH 4.0 or ammonium acetate pH 4.5) was also evaluated and the results showed that its activity was abolished at acidic pHs. Its molecular mass (22,858 Da) was determined by MALDI-TOF and about 90% of its primary structure was verified by high-resolution mass spectrometry using HCD and ETD fragmentations and database search against the sequence of closely related species. It is a novel enzyme which shared high identity with other snake venom metalloproteases (svMPs) belonging to the P-I group. Conclusion: The purification procedure achieved a novel pure highly active metalloprotease from LmrV. This new molecule can help to understand the metalloproteases mechanisms of action, the Lachesis envenoming, as well as to open new perspectives for its use as therapeutic tools.(AU)
Descritores: Peptídeo Hidrolases
Venenos de Serpentes
Lachesis muta
Metaloproteases
Ácido Aspártico Proteases
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-976022
Autor: Cedro, Rafhaella C. A; Menaldo, Danilo L; Costa, Tássia R; Zoccal, Karina F; Sartim, Marco A; Santos-Filho, Norival A; Faccioli, Lúcia H; Sampaio, Suely V.
Título: Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Fonte: J. venom. anim. toxins incl. trop. dis;24:33, 2018. tab, graf, ilus.
Idioma: en.
Projeto: Coordination for the Improvement of Higher Education Personnel (CAPES); . São Paulo Research Foundation (FAPESP); . National Council for Scientific and Technological Development (CNPq); . CAPES; . CNPq/CAPES.
Resumo: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I. Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I showed high catalytic activity, and also inhibitory effects on the ADP-induced platelet aggregation. Its inflammatory characterization showed that BJ-PLA2-I was able to promote leukocyte migration in mice at different concentrations (5, 10 and 20 µg/mL) and also at different response periods (2, 4 and 24 h), mainly by stimulating neutrophil infiltration. Furthermore, increased levels of total proteins, IL-6, IL-1 ß and PGE2 were observed in the inflammatory exudate induced by BJ-PLA2-I, while nitric oxide, TNF-α, IL-10 and LTB4 levels were not significantly altered. This toxin was also evaluated for its cytotoxic potential on normal (PBMC) and tumor cell lines (HL-60 and HepG2). Overall, BJ-PLA2-I (2.5-160 µg/mL) promoted low cytotoxicity, with cell viabilities mostly varying between 70 and 80% and significant values obtained for HL-60 and PBMC only at the highest concentrations of the toxin evaluated. Conclusions: BJ-PLA2-I was characterized as an acidic Asp49 PLA2 that induces acute local inflammation and low cytotoxicity. These results should contribute to elucidate the action mechanisms of snake venom PLA2s.(AU)
Descritores: Bothrops
Venenos de Crotalídeos/síntese química
Citotoxinas
Citotoxicidade Imunológica
Fosfolipases A2/síntese química
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-984690
Autor: Megale, Ângela Alice Amadeu; Magnoli, Fábio Carlos; Kuniyoshi, Alexandre Kazuo; Iwai, Leo Kei; Tambourgi, Denise V; Portaro, Fernanda C. V; Silva, Wilmar Dias da.
Título: Kn-Ba: a novel serine protease isolated from Bitis arietans snake venom with fibrinogenolytic and kinin-releasing activities
Fonte: J. venom. anim. toxins incl. trop. dis;24:38, 2018. graf, tab.
Idioma: en.
Projeto: FAPESP; . CNPq; . CAPES.
Resumo: Bitis arietans is a venomous snake found in sub-Saharan Africa and in parts of Morocco and Saudi Arabia. The envenomation is characterized by local and systemic reactions including pain, blistering, edema and tissue damage, besides hemostatic and cardiovascular disturbances, which can cause death or permanent disabilities in its victims. However, the action mechanisms that provoke these effects remain poorly understood, especially the activities of purified venom components. Therefore, in order to elucidate the molecular mechanisms that make the Bitis arietans venom so potent and harmful to human beings, this study reports the isolation and biochemical characterization of a snake venom serine protease (SVSP). Methods: Solubilized venom was fractionated by molecular exclusion chromatography and the proteolytic activity was determined using fluorescent substrates. The peaks that showed serine protease activity were determined by blocking the proteolytic activity with site-directed inhibitors. In sequence, the fraction of interest was submitted to another cycle of molecular exclusion chromatography. The purified serine protease was identified by mass spectrometry and characterized biochemically and immunochemically. Results: A serine protease of 33 kDa with fibrinogen-degrading and kinin-releasing activities was isolated, described, and designated herein as Kn-Ba. The experimental Butantan Institute antivenom produced against Bitis arietans venom inhibited the Kn-Ba activity. Conclusions: The in vitro activities of Kn-Ba can be correlated with the capacity of the venom to provoke bleeding and clotting disorders as well as hypotension, which are common symptoms presented by envenomed victims. Obtaining satisfactory Kn-Ba inhibition through the experimental antivenom is important, given the WHO's recommendation of immunotherapy in cases of human accidents with venomous snakes.(AU)
Descritores: Venenos de Serpentes
Fibrinogênio
Antivenenos
Substratos para Tratamento Biológico
Serina Proteases
Relatório de Pesquisa
Cininas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-984691
Autor: Benati, Rogério Bodini; Costa, Tássia Rafaela; Cacemiro, Maira da Costa; Sampaio, Suely Vilela; Castro, Fabíola Attié de; Burin, Sandra Mara.
Título: Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, towards leukemic cells
Fonte: J. venom. anim. toxins incl. trop. dis;24:40, 2018. tab, graf, ilus.
Idioma: en.
Projeto: Animal Toxins Research Support Center (NAP-TOXAN- USP); . São Paulo Research Foundation (FAPESP); . Coordination for the Improvement of Higher Education Personnel (CAPES); . National Council for Scientific and Technological Development (CNPq)).
Resumo: A leucemia mieloide crônica (LMC) é uma neoplasia mieloproliferativa BCR-ABL1 + marcada por aumento da mieloproliferação e presença de células leucêmicas resistentes à apoptose. A terapia de primeira linha atual para a LMC é a administração de inibidores da tirosina quinase, mesilato de imatinibe, dasatinibe ou nilotinibe. Embora eficaz no tratamento da LMC, alguns pacientes se tornaram resistentes a essa terapia, levando à progressão da doença e à morte. Assim, a descoberta de novos compostos para melhorar a terapia da LMC ainda é um desafio. Aqui, os destinatários se MjTX-I, uma fosfolipase A 2 isolado a partir de Bothrops moojeni de veneno de cobra, afecta a viabilidade de Bcr-Abl de mesilato de imatinib-resistente + linhas celulares. Métodos: Examinamos o efeito citotóxico e pró-apoptótico de MjTX-I em células K562-S e K562-R Bcr-Abl + e na linha de células HEK-293 não tumorais e células mononucleares de sangue periférico, usando o 3- (4, Brometo de 5-dimetiltiazol-2-il) -2,5-difeniltetrazólio e os métodos de solução fluorescente hipotônica, associados à detecção de ativação de caspases 3, 8 e 9 e clivagem de poli (ADP-ribose) polimerase (PARP). Também analisamos o potencial MjTX-I para modular a expressão de genes relacionados à apoptose em células K562-S e K562-R. Resultados: O MjTX-I diminuiu a viabilidade das células K562-S e K562-R em 60 a 65%, sem afetar a viabilidade das células não tumorais, ou seja, exerceu citotoxicidade seletiva para as linhagens celulares Bcr-Abl + . Em linhas de células leucêmicas, a toxina induziu apoptose, caspases 3, 8 e 9 ativadas, PARP clivada, expressão negativa do gene anti-apoptótico BCL-2 e expressão aumentada do gene pró-apoptótico BAD. Conclusão: O efeito antitumoral de MjTX-I está associado ao seu potencial para induzir apoptose e citotoxicidade em linhagens celulares positivas para Bcr-Abl sensíveis e resistentes ao mesilato de imatinibe, indicando que MjTX-I é um candidato promissor a fármaco para atualizar a terapia de LMC.(AU)
Descritores: Venenos de Serpentes
Leucemia Mieloide/diagnóstico
Bothrops
Citotoxinas/análise
Fosfolipases A2/isolamento & purificação
Neoplasias
-Apoptose
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1149956
Autor: Macedo, Andréia Barcellos Teixeira; Assis, Michelli Cristina Silva de; Milioni, Kelly Cristina; Canto, Débora Francisco do; Souza, Célia Mariana Barbosa de; Chaves, Enaura Helena Brandão.
Título: Elaboration and validation of a protocol for safe administration of enteral nutrition in hospitalized patients / Elaboración y validación de un protocol de administración segura para la nutrición enteral en pacientes hospitalizados / Elaboração e validação de um protocolo para administração segura de nutrição enteral em pacientes hospitalizados
Fonte: Rev. gaúch. enferm;42(spe):e20200181, 2021. tab.
Idioma: en.
Resumo: ABSTRACT Objective: To describe the elaboration and validation of a protocol for administration of enteral nutrition. Method: Validation study by consensus of experts conducted in a university hospital. The construction took place after literature review. The validation was guided by the tool: plan, do, study and act. For validation of the final protocol, 100% consensus was considered. After implementation, preceded by training, evaluation was carried out by using indicators. Results: The protocol describes the actions that guide the nursing team in enteral nutrition. In the validation, there was a 100% consensus on the protocol items. The presential training brought together 425 participants in 80 meetings. After the pilot period, the rate of care for patients with enteral nutrition increased from 39.5% to 73.3%. There was a reduction of 41 hours in the time to release the X-ray report. Conclusion: The protocol provided elements for the prevention of adverse events in patients with enteral nutrition.

RESUMEN Objetivo: Describir la elaboración y validación de un protocolo para la administración de nutrición enteral. Método: Estudio de validación por consenso de expertos realizado en un hospital universitário. La construcción se llevó a cabo después de revisar la literatura. La validación estuvo guiada por la herramienta plan, do, study and act. Para la validación del protocolo final se considero un consenso del 100%. Después de la implementación, precedida por la capacitación, se llevó a cabo la evaluación mediante indicadores. Resultados: El protocolo describe las acciones que orientan al equipo de enfermería en nutrición enteral. En la validación , hubo un consenso del 100% en los ítems del protocolo. La información presencial reunió a 425 participantes en 80 reuniones. Después del período piloto, la tasa de atención a los pacientes con nutrición enteral aumento del 39,5% al 73,3%. Hubo una redución de 41 horas en el tiempo para publicar el informe de rayos X. Conclusión: El protocolo aportó elementos para la prevención de eventos adversos en pacientes con nutrición enteral.

RESUMO Objetivo: Descrever a elaboração e validação de um protocolo para administração de nutrição enteral. Método: Estudo de validação por consenso de especialistas conduzido em um hospital universitário. A construção ocorreu após revisão da literatura. A validação foi norteada pela ferramenta plan, do, study and act. Para validação do protocolo final foi considerado consenso de 100%. Após a implantação, precedida de capacitação, ocorreu a avaliação por meio de indicadores. Resultados: O protocolo descreve as ações que guiam a equipe de enfermagem na nutrição enteral. Na validação, ocorreu consenso de 100% nos itens do protocolo. A capacitação presencial reuniu 425 participantes em 80 encontros. Após o período piloto, a taxa de cuidado ao paciente com nutrição enteral aumentou de 39,5% para 73,3%. Houve redução de 41 horas no tempo para liberação do laudo do raio X. Conclusão: O protocolo forneceu elementos para a prevenção de eventos adversos em pacientes com nutrição enteral.
Descritores: Nutrição Enteral/métodos
Protocolos
Promoção da Saúde
Pacientes Internados
Equipe de Enfermagem
-Prevenção de Doenças
Hospitais Universitários
Limites: Humanos
Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-984692
Autor: Costa, Tássia R; Carone, Sante E. I; Tucci, Luiz F. F; Menaldo, Danilo L; Rosa-Garzon, Nathalia G; Cabral, Hamilton; Sampaio, Suely V.
Título: Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases
Fonte: J. venom. anim. toxins incl. trop. dis;24:37, 2018. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation (FAPESP); . National Council for Scientific and Technological Development (CNPq); . Programa Editoração CAPES; . Programa Editorial CNPq/CAPES.
Resumo: L-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms. Methods and results: The enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at different pH ranges and temperatures. BjussuLAAO-II and BmooLAAO-I demonstrated higher affinity for hydrophobic amino acids, such as Phe and Leu. The two enzymes showed high enzymatic activity in a wide temperature range, from 25 to 75 °C, and presented optimum pH around 7.0. Additionally, Zn2+, Al3+, Cu2+ and Ni2+ ions negatively modulated the enzymatic activity of both LAAOs. As to stability, BjussuLAAO-II and BmooLAAO-I showed high enzymatic activity for 42 days stored at 4°C in neutral pH solution. Moreover, the glycan portions of both LAAOs were analyzed by capillary electrophoresis, which revealed that BjussuLAAO-II presented two main glycan portions with relative masses of 7.78 and 8.13 CGU, while BmooLAAO-I showed three portions of 7.58, 7.94 and 8.37 CGU. Conclusions: Our results showed that, when stored properly, BjussuLAAO-II and BmooLAAO-I present enzymatic stability over a long time period, which is very important to allow the use of these enzymes in pharmacological studies of great impact in the medical field.(AU)
Descritores: Oxirredutases
Polissacarídeos
Venenos de Serpentes
Infecções Bacterianas
Bothrops
Aminoácidos
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-984694
Autor: Jestrzemski, Daniel; Kuzyakova, Irina.
Título: Morphometric characteristics and seasonal proximity to water of the Cypriot bluntnosed viper Macrovipera lebetina lebetina (Linnaeus, 1758)
Fonte: J. venom. anim. toxins incl. trop. dis;24:42, 2018. tab, graf, mapas.
Idioma: en.
Resumo: The blunt-nosed viper Macrovipera lebetina (Linnaeus, 1758) is a medically important snake species in the Middle East. Its nominate subspecies Macrovipera l. lebetina is confined to Cyprus, where it is the only dangerously venomous snake species and heavily pursued. Despite the viper's large size, data on its body mass and sex-specific morphological differences are scarce. It is commonly believed that M. l. lebetina prefers freshwater proximity during summer. Hence, we aimed at investigating M. l. lebetina sex-specific morphological differences and its possible attraction to freshwater bodies in late summer. Methods: Morphometric characteristics, proximity to water and conservation status of M. l. lebetina were investigated in Paphos district (Cyprus) in 2014, 2015 and 2017. Vipers were caught in different habitats, examined morphologically for metric and meristic characters, and released back into their habitat. Additionally, local people were interviewed about the conservation situation of the species. Results: Of 38 recorded blunt-nosed vipers, morphological characteristics were collected from 34 (10 adult males, 16 adult females, eight unsexed juveniles). Rounded total length (ToL) ranged from 23.5 cm to 133.0 cm and weight between 10 g and 1456 g. Adult males significantly exceeded adult females in tail length (TaL), ToL and head length (HL). No significant sex-specific differences were found in snout-vent length (SVL), head width (HW), weight or body condition index (BCI), nor for the ratios TaL / SVL, TaL / ToL, HL / SVL or HL / HW. Adult females from late summer (2015) had a significantly lower mean BCI than those from spring (2014). Distances of blunt-nosed vipers to the nearest water bodies (natural and artificial, respectively) did not differ significantly between spring (2014) and late summer (2015). There was also no significant difference between the distances of vipers to natural and to artificial water bodies in spring (and late summer). Conclusions: Adult male blunt-nosed vipers exceed adult females in TaL, ToL and HL. Adult females are likely in a more vulnerable body condition in late summer than in spring. Periodic drying out of freshwater bodies in summer probably does not affect the species' occurrence. Educational workshops and habitat conservation are recommended for reducing human-viper conflict.(AU)
Descritores: Mordeduras de Serpentes
Serpentes
Água
Toxicophis pugnax
Ecossistema
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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