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Id: lil-660230
Autor: Bittencourt, F; Souza, B. E; Boscolo, W. R; Rorato, R. R; Feiden, A; Neu, D. H.
Título: Benzocaína e eugenol como anestésicos para o quinguio (Carassius auratus) / Benzocaine and eugenol as anesthetics for golden fish (Carassius auratus)
Fonte: Arq. bras. med. vet. zootec;64(6):1597-1602, Dec. 2012. tab.
Idioma: pt.
Resumo: Avaliaram-se os tempos de indução e recuperação de quinguios (Carassius auratus) expostos a dois anestésicos, eugenol e benzocaína. Foram utilizados 128 juvenis com peso médio de 2,07±0,53g e comprimento total médio de 5,51±0,56cm. A benzocaína mostrou ser mais eficiente do que o eugenol em relação ao tempo, tanto para indução ao coma quanto para a recuperação à fuga e também no que diz respeito à sobrevivência. As doses de benzocaína com melhores resultados foram de 87,5 e 100mg.L-1. O eugenol proporcionou demora na indução e na recuperação dos animais, além de ter apresentado mortalidades quando as doses anestésicas foram elevadas.

The aim of this work was to evaluate the induction and recuperation time of quinguio (Carassius auratus) exposed to two anesthetics, eugenol and benzocaine. 128 juveniles with 2.07±0.53g of average weight and 5.51±0.56cm of total length were used. The benzocaine proved to be more efficient than the eugenol regarding the time in inducing a coma and recovering flight, as well as survival. The better results of benzocaine doses were 87.5 and 100mg.L-1. The eugenol resulted in a delay of animal induction and recovery, and also presented mortalities when the anesthetics doses were increased.
Descritores: Benzocaína/análise
Eugenol
Carpa Dourada
-Peixes/anatomia & histologia
Peixes/microbiologia
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  2 / 8 LILACS  
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Castrucci, A. M. L
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Id: lil-556864
Autor: Lopes, G. J. D; Góis, C. C; Lima, L. H. R. G; Castrucci, A. M. L.
Título: Modulation of rhodopsin gene expression and signaling mechanisms evoked by endothelins in goldfish and murine pigment cell lines
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(9):828-836, Sept. 2010. ilus.
Idioma: en.
Resumo: Endothelins (ETs) and sarafotoxins (SRTXs) belong to a family of vasoconstrictor peptides, which regulate pigment migration and/or production in vertebrate pigment cells. The teleost Carassius auratus erythrophoroma cell line, GEM-81, and Mus musculus B16 melanocytes express rhodopsin, as well as the ET receptors, ETB and ETA, respectively. Both cell lines are photoresponsive, and respond to light with a decreased proliferation rate. For B16, the doubling time of cells kept in 14-h light (14L):10-h darkness (10D) was higher compared to 10L:14D, or to DD. The doubling time of cells kept in 10L:14D was also higher compared to DD. Using real-time PCR, we demonstrated that SRTX S6c (12-h treatment, 100 pM and 1 nM; 24-h treatment, 1 nM) and ET-1 (12-h treatment, 10 and 100 pM; 24- and 48-h treatments, 100 pM) increased rhodopsin mRNA levels in GEM-81 and B16 cells, respectively. This modulation involves protein kinase C (PKC) and the mitogen-activated protein kinase cascade in GEM-81 cells, and phospholipase C, Ca2+, calmodulin, a Ca2+/calmodulin-dependent kinase, and PKC in B16 cells. Cells were kept under constant darkness throughout the gene expression experiments. These results show that rhodopsin mRNA levels can be modulated by SRTXs/ETs in vertebrate pigment cells. It is possible that SRTX S6c binding to the ETB receptors in GEM-81 cells, and ET-1 binding to ETA receptors in B16 melanocytes, although activating diverse intracellular signaling mechanisms, mobilize transcription factors such as c-Fos, c-Jun, c-Myc, and neural retina leucine zipper protein. These activated transcription factors may be involved in the positive regulation of rhodopsin mRNA levels in these cell lines.
Descritores: Proliferação de Células/efeitos dos fármacos
Endotelinas/farmacologia
Rodopsina/efeitos dos fármacos
Vasoconstritores/farmacologia
Venenos de Víboras/farmacologia
-Linhagem Celular
Regulação da Expressão Gênica
Carpa Dourada
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/genética
Reação em Cadeia da Polimerase
Proteína Quinase C/efeitos dos fármacos
Proteína Quinase C/genética
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/genética
Rodopsina/genética
Rodopsina/metabolismo
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 8 LILACS  
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Id: lil-519475
Autor: Rema, P; Martins, J.
Título: Crescimento e sobrevivência de larvas de peixe vermelho (Carassius auratus) alimentadas com microdietas comerciais / Growth and survival of Carassius auratus larvae fed commercial micro diets
Fonte: Arq. bras. med. vet. zootec;61(3):714-720, jun. 2009. tab.
Idioma: pt.
Resumo: Para avaliar o desempenho de dois alimentos comerciais para larvas de peixes ornamentais e analisar o efeito que a suplementação em vitaminas e minerais teria sobre a sobrevivência e o crescimento dessas, foi realizado um ensaio de crescimento de 21 dias, com 4800 larvas de peixe vermelho (Carassius auratus), distribuídas por 24 grupos (200 indivíduos por grupo). Cada grupo foi alojado em tanques de 5L de capacidade, ligados a um sistema de recirculação de água. Quatro grupos foram alocados a um controle de jejum, em que os animais não foram alimentados, e os restantes 20 grupos foram utilizados em cinco tratamentos, em quadruplicado. Em cada um dos cinco tratamentos, os peixes ingeriram uma das seguintes dietas: R (referência), C1 (SERA vipan® baby), C2 (SERA® microgran), C1v e C2v (C1 e C2, respectivamente, reforçadas em vitaminas e minerais). As taxas de sobrevivência e crescimento dos peixes do tratamento R foram maiores que as obtidas nos outros tratamentos (P<0,05). Foram ainda observadas diferenças significativas entre tratamentos relativamente ao comprimento e peso totais. Face aos resultados observados, conclui-se que as duas dietas comerciais, com e sem suplementação, não parecem ser adequadas para o preenchimento das necessidades nutricionais de larvas de peixe vermelho.

A 21-day growth trial was performed to evaluate two ornamental fish larvae commercial diets and the effect of mineral and vitamin supplementation of the same commercial microdiets on growth and survival of goldfish larvae (Carassius auratus). A total of 4,800 larvae were randomly distributed in 24 groups of 200 individuals. Each group was housed in a 5L tank, connected to a recirculating water system. Four groups were assigned to a fastening control. The animals in these groups did not receive any food. The remaining 20 groups were distributed in five treatments, in quadruplicate. The animals of each treatment were fed one of five experimental diets: R (reference), C1 (SERA vipan® baby), C2 (SERA® microgran), C1v, and C2v (C1 and C2, respectively, reinforced with minerals and vitamins). At the end of the trial, high survival and growth rates were observed in R treatment. There were significant differences in total length and total weight among dietary treatments. Considering the high survival and growth rates of goldfish larvae obtained with the reference diet, it can be concluded that the two commercial diets tested, with or without mineral and vitamin supplements, do not seem suitable for feeding goldfish larvae.
Descritores: Ração Animal
Carpa Dourada/crescimento & desenvolvimento
Carpa Dourada/metabolismo
Dietoterapia/métodos
Dietoterapia/veterinária
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-505431
Autor: Garção, D. C; Mattioli, R.
Título: L-histidine reduces inhibitory avoidance in Carassius auratus submitted to cerebellar ablation
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;42(1):135-140, Jan. 2009. ilus, graf.
Idioma: en.
Conferência: Apresentado em: Miguel R. Covian Symposium, 4, Ribeirão Preto, May 23-25, 2008.
Projeto: CNPq; . FAPESP.
Resumo: The effect of post-training treatment with L-histidine (LH) on the memory consolidation of inhibitory avoidance was investigated in Carassius auratus submitted to cerebellar ablation. The inhibitory avoidance procedure included 3 days: one habituation day, one training day (5 trials, T1-T5) and one test day. On the training day, each fish was placed individually in a white compartment separated from a black compartment by a sliding door. When the fish crossed into the black compartment, a weight was dropped in front of it (aversive stimulus) and the time to cross was recorded. Saline or LH (100 mg/kg) was injected intraperitoneally 10 min after the trials. Data were log10 transformed and analyzed by ANOVA and the Student-Newman-Keuls test (P < 0.05). In T5, all groups [ablation/LH (N = 15; 189.60 ± 32.52), ablation/saline (N = 14; 204.29 ± 28.95), sham/LH (N = 14; 232.36 ± 28.15), and sham/saline (N = 15; 249.07 ± 25.82)] had similar latencies that were significantly higher than T1 latencies [ablation/LH (89.33 ± 20.41), ablation/saline (97.00 ± 25.16), sham/LH (73.86 ± 18.42), and sham/saline (56.71 ± 17.59)], suggesting acquisition of inhibitory avoidance. For the test, there was a significant reduction in latencies of ablation/LH (61.53 ± 17.70) and sham/saline (52.79 ± 25.37) groups compared to the ablation/saline (213.64 ± 29.57) and sham/LH (199.43 ± 24.48) groups, showing that cerebellum ablation facilitated retention of inhibitory avoidance and LH reversed the effect of ablation. The results support other evidence that LH impairs memory consolidation and/or reduces the interpretation of aversion value.
Descritores: Aprendizagem da Esquiva/efeitos dos fármacos
Cerebelo/cirurgia
Carpa Dourada/fisiologia
Histidina/farmacologia
-Técnicas de Ablação
Carpa Dourada/cirurgia
Tempo de Reação
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-503954
Autor: Valero, Nereida; Meleán, Eddy; Maldonado, Mery; Montiel, Milagros; Larreal, Yraima; Espina, Luz Marina.
Título: Capacidad larvívora del gold fish (Carassius auratus auratus) y del guppy salvaje (Poecilia reticulata) sobre larvas de Aedes aegypti en condiciones de laboratorio / Larvivorous capacity of the gold fish (Carassius auratus auratus) and the wild guppy (Poecilia reticulata) on larvaes of Aedes aegypti in laboratory conditions
Fonte: Rev. cient. (Maracaibo);16(4):414-419, jul.-ago. 2006. tab.
Idioma: es.
Resumo: El control biológico de vectores de importancia médica se presenta como una alternativa al uso de plaguisidas. La utilización de peces larvívoros se promueve como una nueva estratégia para el control de vectores. El propósito de este estudio es evaluar el potencial de dos especies ícticas para el control biológico de mosquitos Aedes aegypti (principal vector de dengue) en condiciones de laboratorio. Un creciente número de larvas de A. aegyptis en estadíos I, II y III (n: 25, 50, y 100) fueron colocados en acuarios con goldfish (Carassius auratus) y guppys salvajes (Poecilia reticulata). Se cuantificó la relación peso corporal/larvas ingeridas/hora en diez ensayos. La especie Poecilia reticulata reflejó una mayor (P<0,001) capacidad larvívora que C. auratus, dado que cuando se administraron 25, 50 y 100 larvas, los Poecilia reticulata obtuvieron valores promedio de eliminación de 3,15 ± 0,12; 5,50 ± 0,31 y 10,95 ± 0,25, respectivamente, frente a 1,21 ± 0,03; 2,45 ± 0,02 y 4,73 ± 0,06 de los C. auratus. Los resultados obtenidos son indicativos de que bajo las condiciones ensayadas, ambas especies de peces poseen alta capacidad de eliminación de larvas de mosquito A. aegypti; sin embargo, consederando su capacidad larvívora, disponibilidad, costo, características reproductivas y resistencia a las condiciones climáticas, la especie Poecilia reticulata ofrece ventajas importantes ante C. auratus auratus cuando ambos se evalúan integralmente como controladores poblacionales de mosquitos vectores.
Descritores: Aedes
Peixes
Carpa Dourada
Larva
Controle Biológico de Vetores
Poecilia
-Venezuela
Medicina Veterinária
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-492173
Autor: Ortega-Salas, A. A; Reyes-Bustamante, H.
Título: Initial sexual maturity and fecundity of the goldfish Carassius auratus (Perciformes: Cyprynidae) under semi-controlled conditions
Fonte: Rev. biol. trop;54(4):1113-1116, dic. 2006. tab.
Idioma: en.
Resumo: We studied the time of initial sexual maturity and the fecundity of the goldfish Carassius auratus under semi-controlled conditions. During the study the water temperature fluctuated from 21 to 30 degrees C, the pH between 7 and 8, and the dissolved oxygen between 5.5 and 7 ppm. Sixty days old juvenile fish received prophylactic treatments of brackish water (15 per thousand) and methylene blue baths (1%), and they were fed with live Daphnia magna. The initial sexual maturity occurred between 225 and 233 days of captivity. Mean absolute fecundity was 2,347 (+/- 302.2 SD) ova per gonad, and mean relative fecundity was 63.44 eggs/g fish weight. Gonadosomatic index was between 7.5 and 9.0. Our data will allow ornamental fish producers to calculate the time of initial maturity and fecundity for this species.

Estudiamos el tiempo inicial de madurez sexual y la fecundidad del pez dorado Carassius auratus en condiciones semi-controladas. Durante el estudio la temperatura del agua varió de 21 a 30 oC, el pH entre 7 y 8, y el oxígeno disuelto entre 5.5 y 7 ppm. Jóvenes de 60 días de edad recibieron tratamiento profiláctico con agua salobre (15 ‰), y baños de azul de metileno (1 %). Se les alimentó con Daphnia magna. La madurez sexual inicial fue tras 225 a 233 días en cautiverio. La fecundidad absoluta media fue de 2 347 (±302.2 DS) óvulos por gónada y la fecundidad relativa fue de 63.44 óvulos/g del peso del pez. El índice gonadosomático se calculó entre 7.5 and 9.0.
Descritores: Carpa Dourada/fisiologia
Fertilidade/fisiologia
Maturidade Sexual/fisiologia
-Carpa Dourada/crescimento & desenvolvimento
Fatores de Tempo
Limites: Animais
Feminino
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-484438
Autor: Faganello, F. R; Mattioli, R.
Título: Chlorpheniramine facilitates inhibitory avoidance in teleosts submitted to telencephalic ablation
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(5):398-402, May 2008. graf, ilus.
Idioma: en.
Resumo: The present study investigated the involvement of H(1) histaminegic receptor on the acquisition of inhibitory avoidance in Carassius auratus submitted to telencephalic ablation. The fish were submitted to telencephalic ablation 5 days before the experiment. The inhibitory avoidance procedure included 1 day for habituation, 3 days for training composed of 3 trials each (1st day: T1, T2, T3; 2nd day: 2T1, 2T2, 2T3; 3rd day: 3T1, 3T2, 3T3) and 1 day for test. On training days, the fish were placed in a white compartment, after 30 s the door was opened. When the fish crossed to a black compartment, a weight was dropped (aversive stimuli). Immediately after the third trial, on training days, the fish received, intraperitoneally, one of the pharmacological treatments (saline (N = 20), 8 (N = 12) or 16 (N = 13) µg/g chlorpheniramine, CPA). On the test day, the time to cross to the black compartment was determined. The latency of the saline group increased significantly only on the 3rd trial of the 2nd training day (mean ± SEM, T1 (50.40 ± 11.69), 2T3 (226.05 ± 25.01); ANOVA: P = 0.0249, Dunn test: P < 0.05). The group that received 8 µg/g CPA showed increased latencies from the 2nd training day until the test day (T1 (53.08 ± 17.17), 2T2 (197.75 ± 35.02), test (220.08 ± 30.98); ANOVA: P = 0.0022, Dunn test: P < 0.05)). These results indicate that CPA had a facilitating effect on memory. We suggest that the fish submitted to telencephalic ablation were able to learn due to the local circuits of the mesencephalon and/or diencephalon and that CPA interferes in these circuits, probably due an anxiolytic-like effect.
Descritores: Aprendizagem da Esquiva/efeitos dos fármacos
Clorfeniramina/farmacologia
Carpa Dourada/fisiologia
Antagonistas dos Receptores Histamínicos H1/farmacologia
Telencéfalo/fisiologia
-Análise de Variância
Aprendizagem da Esquiva/fisiologia
Condicionamento Clássico/efeitos dos fármacos
Condicionamento Clássico/fisiologia
Condicionamento Operante/efeitos dos fármacos
Condicionamento Operante/fisiologia
Memória/efeitos dos fármacos
Memória/fisiologia
Retenção Psicológica
Telencéfalo/efeitos dos fármacos
Telencéfalo/cirurgia
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-163892
Autor: Mattioli, R; Coelho, J; Martins, A.
Título: Reinforcing properties of the substance P C-fragment analog DiMe-C7 in Carassius auratus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;29(4):495-9, Apr. 1996. graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: The aim of the present study was to investigate whether two substance P (SP) fragments have reinforcing effects in Carassius auratus when the fish were tested in a place-preference experimental model. Fish were placed in a 3-compartment box in which one compartment gives access to two others that are not connected. The time spent in each compartment was recorded for 10 min in order to determine which one was preferred. Twenty-four hours later the fish were given one of the following ip treatments: 1) group VEH (N = 12), injected with teleost saline, 2) group DiMe-C7 (N = 12), injected with 33 mug/kg DiMe-C7, and 3) group SP1-7 (N = 12), injected with 167 mug/kg SP1-7. Immediately after treatment the fish were kept for 30 min in the compartment that was the least preferred on the day before and this procedure was repeated for 3 days. On the fifth day the fish were retested for 10 min to determine the time spent in each compartment. Two-way analysis of variance with treatments and testing as factors indicated a main effect (P<0.0025) as well as a testing effect (P<0.009). The post-hoc Scheffé multiple comparison test indicated that only the DiMe-C7 group presented an increase in the time spent in the paired compartment after treatment. We suggest that the C-tenninal fragment of SP has reinforcing effects in Carassius auratus.
Descritores: Comportamento Animal/efeitos dos fármacos
Substância P/farmacologia
-Análise de Variância
Carpa Dourada/fisiologia
Substância P/administração & dosagem
Limites: Animais
Responsável: BR1.1 - BIREME



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