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Id: lil-755539
Autor: Namulawa, V. T; Kato, C. D; Nyatia, E; Rutaisire, J; Britz, P. J.
Título: Transmission electron microscopy of the gastrointestinal tract of Nile perch Lates niloticus / Uso de microscopía electrónica de transmisión para visualizar el tracto gastrointestinal de la perca del Nilo Lates niloticus
Fonte: Int. j. morphol;33(2):751-758, jun. 2015. ilus.
Idioma: en.
Resumo: The ultrastructure of the gastrointestinal tract of Nile perch was described using Transmission Electron Microscopy standard procedures. Investigations revealed the presence of mucous cells, blood vessels and oil droplets plus several nerve cells and muscle bundles in the oral cavity. Further observations revealed columnar epithelial cells in the oesophagus, with a ragged surface, high electron dense cytoplasm, intercellular spaces, mitochondria and mucus granules. The lamina propria of the oesophagus was composed of loose connective tissue, blood vessels, nerve cells and several leucocytes, while the muscularis externa was composed of striated muscle bundles with the a Z line, A band and I band. The stomach was characterized by mucosal glandular cells with electron dense granules, a prominent nucleus, mitochondria, and secretory canaliculi. The liver had an isotropic parenchyma composed of several hepatocytes with a single spherical euchromatic nucleus. The exocrine pancreatic cells in the liver occurred around large blood vessels, had large centrally positioned nuclei with electron dense nucleolus, electron dense granules, Rough Endoplasmic Reticulum, and mitochondria which dominated the cytoplasm of pancreatic cells.

La ultraestructura del tracto gastrointestinal de la perca del Nilo fue descrita utilizando un procedimiento estándar de Microscopía Electrónica de Transmisión (MET). Las investigaciones revelaron la presencia de células mucosas, vasos sanguíneos y pequeñas gotas de aceite, además de varias células nerviosas y haces musculares en la cavidad oral. Nuevas observaciones revelaron células epiteliales columnares en el esófago, con una superficie irregular, citoplasma elevado y electrodenso, espacios intercelulares, mitocondrias y gránulos mucosos. La lámina propia del esófago se compone de tejido conectivo laxo, vasos sanguíneos, células nerviosas y numerosos leucocitos, mientras que la muscular externa se compone de haces musculares estriados, con presencia de la línea Z, banda A y banda I. El estómago se caracteriza por presentar células glandulares mucosas con gránulos electrodensos , un núcleo prominente, mitocondrias y canalículos secretores. El hígado presentó un parénquima isotrópico compuesto de varios hepatocitos con un solo núcleo eucromático y esférico. Las células pancreáticas exocrinas se encontraron alrededor de grandes vasos sanguíneos, presentando grandes núcleos posicionados centralmente, con nucleólos densos, gránulos electrodensos en el retículo endoplasmático rugoso, y mitocondrias, que dominaron el citoplasma de las células pancreáticas.
Descritores: Trato Gastrointestinal/ultraestrutura
Microscopia Eletrônica de Transmissão
Percas/anatomia & histologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  2 / 10 LILACS  
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Id: lil-695002
Autor: Namulawa, Victoria Tibenda; Kato, Charcles Drago; Nyatia, Edward; Rutaisire, Justus; Britz, Peter.
Título: Scanning electron microscopy of the gastrointestinal tract of nile perch (lates niloticus, linneaus, 1758) / Microscopía electrónica de barrido del tracto gastrointestinal de la perca del nilo (lates niloticus, linnaeus, 1758)
Fonte: Int. j. morphol;31(3):1068-1075, set. 2013. ilus.
Idioma: en.
Resumo: The morphology of the gastrointestinal tract of adult Nile perch was described using standard SEM procedures. Investigations revealed the presence of cardiform teeth in the oral cavity, goblet cells and finger print-like microridges on the hard palate and oesophagus lumenal surface. Elaborate patterns and bacterial cells were observed on the stomach lumenal surface and intense foldings in the intestinal region. These observations provide a better understanding of the morphology of the gut in Nile perch and how it is suited for its digestive function.

El objetivo fue describir la morfología del tracto gastrointestinal de la perca del Nilo adulta mediante microscopía electrónica de barrido estándar. La investigación reveló la presencia de dientes cardiformes en la cavidad oral, células caliciformes y microcrestas como huellas digitales en el paladar duro y la superficie luminal del esófago. Se observaron patrones elaborados, así como bacterias en la superficie luminal del estómago, y plegamientos marcados en la región intestinal. Estas observaciones proporcionan una mejor comprensión de la morfología del intestino de la perca del Nilo y como se adapta para su función digestiva.
Descritores: Microscopia Eletrônica de Varredura
Percas/anatomia & histologia
Trato Gastrointestinal/ultraestrutura
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  3 / 10 LILACS  
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Texto completo SciELO Chile
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Id: lil-627027
Autor: Yakubu, A; Okunsebor, S A.
Título: Morphometric differentiation of two Nigerian fish species (Oreochromis niloticus and Lates niloticus) using principal components and discriminant analysis / Diferenciación morfométrica de dos especies de peces nigerianos (Oreochromis niloticus y Lates niloticus) utilizando análisis de componentes principales y análsis discriminante
Fonte: Int. j. morphol;29(4):1429-1434, dic. 2011. ilus.
Idioma: en.
Resumo: Morphological differences between two close fish populations were investigated in Doma Dam, north central Nigeria using principal component and discriminant analyses. The purpose was to identify the two species correctly and assign them into distinct genetic groups. To achieve this, seven morphometric measurements (body weight, standard length, total length, head length, body depth, dorsal fin length and caudal fin length) were made on each of the studied 252 fish specimens (153 Oreochromis niloticus and 99 Lates niloticus). The analysis of variance showed that significantly (P<0.05) higher values were recorded for Lates niloticus in all the body traits examined, except dorsal fin length. From the principal component analysis, the differences between the species resulted mainly from dorsal fin length, body weight, caudal fin length and head length. The stepwise discriminant analysis revealed that standard length, dorsal fin length and caudal fin length were the three most discriminating variables useful to distinguish clearly the two species at mature age. These three variables with strong discriminating power correctly classified 98.0 percent of individuals from the sample of known-fish populations. The classification accuracy was cross-validated using split-sample method, and indicated a 97.6 percent success rate (98.0 percent of Oreochromis niloticus and 97.0 percent of Lates niloticus were correctly assigned). The results can provide a sound scientific basis for the effective management and sustainable exploitation of the genetic resources of the two species under subtropical conditions.

Las diferencias morfológicas entre dos poblaciones de peces similares se han investigado en Doma Dam, centro-norte de Nigeria utilizando análisis de componentes principales y análisis discriminante. El propósito fue identificar correctamente las dos especies y asignarlas a los distintos grupos genéticos. Para lograr esto, siete medidas morfométricas (peso corporal, longitud estándar, longitud total, longitud de la cabeza, profundidad del cuerpo, longitud de la aleta dorsal y la longitud de la aleta caudal) se realizaron en cada una de las muestras de los 252 peces estudiados (153 Oreochromis niloticus y 99 Lates niloticus) . El análisis de varianza mostró que de forma significativa (p<0,05) los valores más altos se registraron en Lates niloticus, en todos los rasgos del cuerpo examinado con excepción de la longitud de la aleta dorsal. A partir del análisis de componentes principales, las diferencias entre las especies se observó principalmente en la longitud de la aleta dorsal, el peso corporal, la longitud de la aleta caudal y longitud de la cabeza. El análisis discriminante reveló que la longitud estándar, longitud aleta dorsal y caudal son las tres variables más discriminatorias de utilidad para distinguir claramente las dos especies en la edad madura. Estas tres variables con fuerte poder de discriminación clasificaron correctamente el 98,0 por ciento de los individuos de la muestra de las poblaciones conocidas de peces. La exactitud de la clasificación fue sometida a una validación cruzada utilizando el método de la muestra dividida, la que indicó una tasa de éxito del 97,6 por ciento (98,0 por ciento de Oreochromis niloticus y 97,0 por ciento de Lates niloticus asignados correctamente). Los resultados pueden proporcionar una base científica sólida para el manejo eficaz y la explotación sostenible de los recursos genéticos de las dos especies en condiciones subtropicales.
Descritores: Ciclídeos/anatomia & histologia
Percas/anatomia & histologia
-Conservação dos Recursos Naturais
Análise Discriminante
Genótipo
Análise Multivariada
Nigéria
Análise de Componente Principal
Perciformes/anatomia & histologia
Perciformes/classificação
Especificidade da Espécie
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  4 / 10 LILACS  
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Texto completo SciELO Brasil
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Id: lil-343402
Autor: Chellappa, S; Câmara, M. R; Chellappa, N. T; Beveridge, M. C. M; Huntingford, F. A.
Título: Reproductive ecology of a neotropical cichlid fish, Cichla monoculus (Osteichthyes: Cichlidae)
Fonte: Braz. j. biol;63(1):17-26, Feb. 2003. ilus, tab, graf.
Idioma: en.
Resumo: The reproductive ecology of the freshwater fish Cichla monoculus Spix, 1831 (Osteichthyes: Cichlidae) was investigated in the Campo Grande Reservoir, Northeast Brazil. Rainfall, temperature, pH, dissolved oxygen and electrical conductivity of the water were recorded monthly. Fish collected on a monthly basis were measured, weighed, dissected, sexed and the stage of maturation of the gonads were assessed by macro and microscopic means. The semi-arid study region has short spells of rain of 2-3 months duration interspersed with dry seasons. A positive correlation was observed between rainfall and air and water temperatures and conductivity of the water. The study population had an extended spawning season, with peak reproductive activity coinciding with low water temperatures. Males were longer and heavier than females on average and were larger at onset of sexual maturity. The size frequency distributions of the oocytes indicate that C. monoculus is a multiple spawner with an estimated batch fecundity of 3100. Condition factor showed an inverse relationship in relation to gonad size during maturation in both sexes and spent fish were in poor condition. In mature males, lipid stores in the post-occipital cephalic protuberance, a secondary sexual characteristic developed during the reproductive phase, which depleted in spent individuals. The success of this fish is attributed to its reproductive capacity and to the phenotypic plasticity that allows it to adapt to the harsh ecological conditions that prevail in the semi-arid region of Northeast Brazil
Descritores: Ecologia
Gônadas
Percas
Reprodução
Maturidade Sexual
-Condutividade Elétrica
Meio Ambiente
Água Doce
Concentração de Íons de Hidrogênio
Percas
Estações do Ano
Temperatura
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


  5 / 10 LILACS  
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Id: lil-335886
Autor: Pandolfi, Matias; Paz, Dante A; Maggese, Cristina; Meijide, Fernando J; Vissio, Paual G.
Título: Immunocytochemical localization of different cell types in the adenohypophysis of the cichlid fish Cichlasoma dimerus (Heckel, 1840)
Fonte: Biocell;25(1):35-42, Apr. 2001.
Idioma: en.
Resumo: The adenohypophysis of the cichlid fish Cichlasoma dimerus was studied using the avidin-biotin-peroxidase method with antisera raised against piscine pituitary hormones and heterologous antisera against mammalian pituitary hormones. Antiserum raised against rabbit ACTH recognized a group of cells bordering the neurohypophysis (NH) in the rostral pars distalis (RPD). Anti-chum salmon prolactin (PRL) identified a compact group of cells in the periphery of the RPD. Gonadotropin II (GTH II), thyrotropin (TSH) and growth hormone (GH)-ir cells were localized in the proximal pars distalis. Ir-GTH II cells were also observed in the dorsal area of the pars intermedia (PI). Ir-GTH I cells could not be identified using anti-chum salmon GTH I, this may be due either to a failure of the antisera to recognize the gonadotropin or to a low expression of the hormone in adults of this species. PAS positive cells from the PI bound specifically with three different antisera raised against somatolactin (SL) of four different fish species. These cells surrounded deep branches of the NH in the PI.
Descritores: Adeno-Hipófise/química
Adeno-Hipófise/citologia
Percas
-Hormônio Adrenocorticotrópico
Glicoproteínas/análise
Gonadotropinas
Hormônio do Crescimento/análise
Hormônios Hipofisários/análise
Imuno-Histoquímica
Neurônios/citologia
Prolactina
Tireotropina
Limites: Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 10 LILACS  
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Texto completo SciELO Brasil
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Id: lil-335614
Autor: Gibran, F. Z.
Título: The sea basses Diplectrum formosum and D. radiale (Serranidae) as followers of the sea star Luidia senegalensis (Asteroidea) in Southeastern Brazil
Fonte: Braz. j. biol;62(4a):591-594, Nov. 2002. ilus.
Idioma: en.
Projeto: FAPESP.
Resumo: This paper reports on Luidia senegalensis being followed by Diplectrum formosum and D. radiale in shallow soft bottoms of southeastern Brazilian coast, and comments on this association between bottom-dwelling organisms and serranids. Following is likely due to the serranid's inquisitive behaviour, and both juvenile and adult sea basses acted as followers, feeding on disturbed or exposed prey due to sea star activities
Descritores: Comportamento Alimentar
Percas
Estrelas-do-Mar
-Comportamento Animal
Brasil
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  7 / 10 LILACS  
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Id: lil-285844
Autor: Wilhelm Filho, D; Torres, M. A; Tribess, T. B; Pedrosa, R. C; Soares, C. H. L.
Título: Influence of season and pollution on the antioxidant defenses of the cichlid fish acará (Geophagus brasiliensis)
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(6):719-26, Jun. 2001. ilus, graf.
Idioma: en.
Resumo: The livers of Geophagus brasiliensis collected from both a non-polluted site and a polluted site were analyzed for different antioxidant defenses, O2 consumption, thiobarbituric acid-reactive substance (TBARS) levels, and histological damage. Compared to controls (116.6 + or - 26.1 nmol g-1), TBARS levels were enhanced at the polluted site (284.2 + or - 25.6 nmol g-1), as also was oxygen consumption (86.6 + or - 11.3 and 128.5 + or - 9.8 µmol O2 min-1 g-1, respectively). With respect to enzymatic antioxidants, increased catalase activities (8.7 + or - 1.3 and 29.2 + or - 2.4 mmol min-1 g-1, respectively), unchanged superoxide dismutase activities (767.2 + or - 113.3 and 563.3 + or - 70.2 U g-1, respectively), and diminished glutathione S-transferase activities (29.0 + or - 3.2 and 14.9 + or - 3.2 µmol min-1 g-1, respectively) were detected. Reduced glutathione (1.91 + or - 0.17 and 1.37 + or - 0.25 mM, respectively), oxidized glutathione (1.50 + or - 0.20 and 0.73 + or - 0.17 mM, respectively), and total glutathione (3.40 + or - 0.26 and 2.07 + or - 0.27 mM, respectively) concentrations were also below control values at the polluted site. Nevertheless, the observed ethoxyresorufin-O-deethylase activities (1.34 + or - 0.11 and 16.7 + or - 0.21 pmol min-1 mg-1, respectively) showed enhanced values at the polluted site. The main histological damage observed in the hepatocytes from fish collected at the polluted site was characterized by heavy lipid infiltration. Fish collected at the end of spring showed higher O2 consumption, higher superoxide dismutase and glutathione S-transferase activities, and higher total and oxidized glutathione concentrations compared to the beginning of autumn. No seasonal changes were observed in catalase activities, glutathione or TBARS levels. Fish chronically exposed to relatively high pollution levels seem to be unable to set up adequate antioxidant defenses, probably due to severe injury to their hepatocytes. The higher antioxidant defenses found at the end of spring are probably related to the enhanced activities during high temperature periods in thermoconforming organisms.
Descritores: Adaptação Fisiológica
Antioxidantes/metabolismo
Percas/fisiologia
Estações do Ano
Poluição da Água
-Glutationa/metabolismo
Fígado/metabolismo
Fígado/patologia
Oxirredutases/metabolismo
Consumo de Oxigênio
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
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Id: lil-225928
Autor: Zylber, María Inés; Nuñez, Margarita Ostrowski de.
Título: Some aspects of the development of Lobatostoma jungwirth Kritscher, 1974 (Aspidogastrea) in snails and cichlid fishes from Buenos Aires, Argentina
Fonte: Mem. Inst. Oswaldo Cruz;94(1):31-5, Jan.-Feb. 1999. ilus, tab, graf.
Idioma: en.
Resumo: The mollucs Heleobia castellanosae and Ampullaria canaliculata and cichlid fishes were collected from an artificial pond at Tres de Febrero Park (Buenos Aires city), between June 1994 and May 1995. One hundred and eight of 2,467 H. castellanosae examined were infected with pre-adults of Lobatostoma jungwirthi, 112 of them in concurrent infections with digeneans. L. jungwirthi was significantly more prevalent in larger snails, specially those infected with larval digeneans, but the prevalence of infection did not fluctuate significantly throughout the year. A. canaliculata and cichlids smaller than 10 cm were not infected, but larger Cichlasoma facetum harboured adults of L. jungwirthi in their intestines. Experimental infections of C. facetum and Gymnogeophagus meridionalis with pre-adult aspidogastreans obtained from H. castellanosae were successful. The development of L. jungwirthi in the snail host involves five arbitrary growth phases. Its life-cycle is heteroxenous, similar to that described for L. manteri, with H. castellanosae as the intermediate host and cichlid fishes as the definitive host in this pond.
Descritores: Peixes/parasitologia
Moluscos/parasitologia
Percas/parasitologia
Caramujos/parasitologia
Trematódeos
-Argentina
Limites: Animais
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas


  9 / 10 LILACS  
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Val, A. L
Id: lil-161528
Autor: Almeida-Val, V. M. F; Farias, I. P; Silva, M. N. P; Duncan, W. P; Val, A. L.
Título: Biochemical adjustments to hypoxia by Amazon cichlids
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;28(11/12):1257-63, Nov.-Dec. 1995. tab, graf.
Idioma: en.
Conferência: Apresentado em: International Symposium on Regulatory Mechanisms of Cardiovascular and Respiratory Function in Vertebrates, Säo Carlos, Sept. 8-13, 1994.
Projeto: CNPq; . INPA.
Resumo: The isozyme distribution of cichlid lactate dehydrogenase (LDH) is related to species environmental preferences. Cichlasoma amazonarum. occurs in different environments and presents LDH tissue distribution patterns that correlate with oxygen tension at the capture location. Cichlasoma amazonarum was exposed to long term severe hypoxia (51 days at 36.4 +/- 5.9 mmHg), tissue LDH isozyme distribution was analyzed by electrophoresis and enzyme activities were measured by monitoring the oxidation of NADH as pyruvate was reduced to lactate. The exposure of Cichlasoma amazonarum to long-term severe hypoxia resulted in changes in the tissue distribution of LDH isozymes. The major changes in response to hypoxia occurred in heart, liver and brain: isozyme A4 was activated in heart and brain, whereas isozyme B4 was activated in liver. The most significant quantitative change occurred in brain LDH of hypoxia-exposed animals which adopted muscle type kinetics, reflecting a new LDH isozyme distribution. LDH activity was significantly reduced (P<0.05) in animals exposed to hypoxia (N = 8), suggesting an overall LDH suppression. Pyruvate inhibition decreased in all hypoxia-exposed tissues. Thus, the ability of Cichlasoma amazonarum to regulate LDH tissue expression according to oxygen availability allows the animal to survive chronic hypoxic environments. This phenotypic plasticity may occur in other hypoxia-tolerant fish species.
Descritores: Hipóxia/metabolismo
L-Lactato Desidrogenase/metabolismo
Percas/metabolismo
-Análise de Variância
Cérebro/enzimologia
Eletroforese
Fígado/enzimologia
L-Lactato Desidrogenase/metabolismo
Músculo Esquelético/enzimologia
Miocárdio/enzimologia
Limites: Animais
Tipo de Publ: Revisão
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 10 LILACS  
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Andrade, Roberto Milward de
Id: lil-73263
Autor: Feitosa, Vilma Ramos; Andrade, Roberto Milward de.
Título: Atividade predatória de Astronotus ocellatus (Cichlidae) sobre Biomphalaria glabrata (Planorbidae) / Predatory activity of Astronotus ocellatus (Cichlidae) on Biomphalaria glabrata (Planorbidae)
Fonte: Rev. bras. malariol. doenças trop;38:19-27, 1986. ilus, tab.
Idioma: pt.
Resumo: Foram realizados experimentos em laboratório com o Astronotus ocellatus, predador de Biomphalaria glabrata, objetivando determinar a influência do peixe sobre o ritmo de desovas do molusco hospedeiro da esquistossomose, ademais de sua eficácia predatória, tanto em relaçäo a B. glabrata, quanto a peixes do gênero Poecilia. Constatou-se que A. ocellatus parece näo predar as cápsulas oviferas do caramujo, mas apenas o molusco. Também verificou-se que a predaçäo foi estatisticamente mais significativa para B. glabrata do que para Poecilia. Os resultados desse experimento sugerem que a maior predaçäo dos caramujos decorreu do fato de se constituirem presas mais fáceis que os peixes do gênero Poecilia
Descritores: Biomphalaria
Percas
Controle Biológico de Vetores
Comportamento Predatório
Limites: Animais
Responsável: BR1.1 - BIREME



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