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Id: lil-790570
Autor: Maia, Anny Caroline Lopes; Catão, Maria Helena Chaves de Vasconcelos.
Título: Clareamento Dental Laser (470 nm) e Led com Peróxido de Hidrogênio / Whitening using Laser and Led with 35% Hydrogen Peroxide
Fonte: Rev. bras. ciênc. saúde;14(1):99-108, 2010. tab, graf.
Idioma: pt.
Resumo: Avaliar “in vitro” a efetividade do clareamento dentalusando o Laser e o LED com peróxido de hidrogênio a 35%em dentes bovinos escurecidos artificialmente. Material eMétodos: Os dentes foram submetidos a um processo deescurecimento in vitro os quais foram divididos em doisgrupos. Grupo 1. Peróxido de hidrogênio a 35% ativado peloLED, grupo 2 peróxido de hidrogênio a 35% ativado peloLaser de clareamento dental. A avaliação foi feita por doisavaliadores calibrados através de escores, antes domanchamento, depois do manchamento e depois doclareamento.observação visual. Resultados: Os resultadosmostraram que os métodos apresentaram diferençasestatisticamente tanto em relação ao LED e ao Laser noclareamento dental e em relação à tonalidade, apresentandomaior efetividade o peróxido de hidrogênio ativado pelo Laser.Conclusão: as técnicas ativadas por LED ou Laseralcançaram um alto nível de clareamento sendo que o Laserapresentou resultados superiores tanto em clareamento comoem tonalidade comparado ao LED...

To Evaluate “in vitro” the effectiveness of whiteningusing Laser and LED with 35% hydrogen peroxide in bovinedarkened teeth artificially. Material and methods: The teethwere subjected to processes of darkening in vitro whichwere divided into two groups. Group 1. 35% hydrogenperoxide activated by led, group 2 35% hydrogen peroxidepowered Laser whitening. The evaluation was done by twoevaluators calibrated through scores prior to staining, afterstaining and after bleaching. Visual observation. Results:The results showed that the methods presented statisticallydifferences from both the LED and laser whitening andtonality, showing greater effectiveness hydrogen peroxideactivated by Laser. Conclusion: the techniques enabled byLaser or LED reached a high level of whitening being whatLaser presented superior results in both whitening as intonality compared to LED...
Descritores: Clareamento Dental
Lasers
Peróxido de Hidrogênio
Limites: Bovinos
Tipo de Publ: Estudo Clínico
Responsável: BR8.1 - Biblioteca Central


  2 / 5564 LILACS  
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Id: biblio-951802
Autor: Djordjevic, Jasna; Boskovic, Marija; Starcevic, Marija; Ivanovic, Jelena; Karabasil, Nedjeljko; Dimitrijevic, Mirjana; Brankovic Lazic, Ivana; Baltica, Milan Z.
Título: Survival of Salmonella spp. in minced meat packaged under vacuum and modified atmosphere
Fonte: Braz. j. microbiol;49(3):607-613, July-Sept. 2018. tab.
Idioma: en.
Resumo: Abstract The effect of different modified atmosphere packaging regimes on the behavior of Salmonella spp. on minced meat was studied. Minced meat was experimentally contaminated with a Salmonella spp. cocktail (S. Enteritidis, S. Typhimurium, S. Infantis and S. Arizonae), packaged under vacuum or modified atmosphere with initial headspaces containing 20%O2/50%CO2/30%N2 and 20%O2/30%CO2/50%N2) and stored at 3 ± 1 °C for 12 days. Samples were analyzed for Salmonella spp., viable and lactic acid bacteria count every third day. Salmonella spp. counts decreased during storage in all packaging types, with reductions of about 1.5 log CFU/g. A significant difference (p < 0.01) was noted between Salmonella spp. counts in meat packaged in vacuum and modified atmospheres, although there was no significant difference in Salmonella spp. count between meat packaged in 50%CO2, and meat packaged in 30%CO2. At the end of the study, there were significant differences (p < 0.01; p < 0.05) in total viable and lactic acid bacterial counts between meat packaged in vacuum and modified atmosphere, and the lowest counts were noted in meat packaged in modified atmosphere with 50%CO2.
Descritores: Salmonella/crescimento & desenvolvimento
Embalagem de Alimentos/métodos
Viabilidade Microbiana
Carne/microbiologia
-Salmonella/isolamento & purificação
Salmonella/genética
Suínos
Vácuo
Contagem de Colônia Microbiana
Embalagem de Alimentos/instrumentação
Carne/análise
Limites: Animais
Bovinos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  3 / 5564 LILACS  
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Mathias, Luis Antonio
Machado, Rosangela Zacarias
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Id: biblio-951797
Autor: Bastos, Carla Resende; Mathias, Luis Antonio; Jusi, Márcia Mariza Gomes; Santos, Renata Ferreira dos; Silva, Glaucenyra Cecília Pinheiro da; André, Marcos Rogério; Machado, Rosangela Zacarias; Bürger, Karina Paes.
Título: Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
Fonte: Braz. j. microbiol;49(3):564-568, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the conjugate at 1:30,000. The comparison of the dot-blot results with 2-ME showed Kappa index of 0.9939, sensitivity of 99.48%, and specificity 99.91%, with CF, Kappa index of 0.8226, sensitivity 100% and specificity 95.32%. Using the combination of the test results 2-ME and CF to establish the true condition of the animal, the dot-blot showed relative sensitivity of 100%, and relative specificity of 99.91%. The evaluated test proved to be effective and reliable, besides being easy to handle and interpret the results.
Descritores: Brucella abortus/isolamento & purificação
Brucelose/veterinária
Testes Sorológicos/métodos
Doenças dos Bovinos/diagnóstico
Anticorpos Antibacterianos/sangue
-Brucella abortus/imunologia
Brucelose/diagnóstico
Brucelose/microbiologia
Brucelose/sangue
Testes Sorológicos/instrumentação
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/sangue
Sensibilidade e Especificidade
Limites: Animais
Bovinos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-951794
Autor: Hernandez-Flores, Jose Luis; Pérez, Juan Caballero; Gutiérrez, Carlos Saldaña; Hernández, Andrés Cruz; Alonso, Gerardo Soto; Hernández, Sergio Pacheco; Gómez, Sergio Romero; Fernández, Francisco; Loske, Achim M; Guillén, Juan Campos.
Título: pMEX01, a 70kb Plasmid Isolated From Escherichia Coli That Confers Resistance to Multiple ß-Lactam Antibiotics
Fonte: Braz. j. microbiol;49(3):569-574, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The strain CM6 is of high epidemiological concern because it not only displays resistance to multiple β-lactam antibiotics but also to other kinds of antibiotics.
Descritores: Plasmídeos/genética
Doenças dos Bovinos/microbiologia
Farmacorresistência Bacteriana
beta-Lactamas/farmacologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Infecções por Escherichia coli/veterinária
Antibacterianos/farmacologia
-Plasmídeos/metabolismo
beta-Lactamases/genética
beta-Lactamases/metabolismo
Testes de Sensibilidade Microbiana
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Escherichia coli/metabolismo
Infecções por Escherichia coli/microbiologia
México
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  5 / 5564 LILACS  
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Miagostovich, Marize Pereira
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Id: biblio-974321
Autor: Melgaço, Fabiana Gil; Corrêa, Adriana Abreu; Ganime, Ana Carolina; Brandão, Marcelo Luiz Lima; Medeiros, Valéria de Mello; Rosas, Carla de Oliveira; Lopes, Silvia Maria dos Reis; Miagostovich, Marize Pereira.
Título: Evaluation of skimmed milk flocculation method for virus recovery from tomatoes
Fonte: Braz. j. microbiol;49(supl.1):34-39, 2018. tab, graf.
Idioma: en.
Projeto: Ministério da Ciência, Tecnologia e Informação/Conselho Nacional de Desenvolvimento Científico e Tecnológico/Agência Nacional de Vigilância Sanitária.
Resumo: Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.
Descritores: Vírus/isolamento & purificação
Lycopersicon esculentum/química
Leite/química
Microbiologia de Alimentos/métodos
Frutas/virologia
-Vírus/classificação
Vírus/genética
Lycopersicon esculentum/classificação
Lycopersicon esculentum/virologia
Floculação
Microbiologia de Alimentos/instrumentação
Frutas/classificação
Frutas/química
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  6 / 5564 LILACS  
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Id: biblio-974318
Autor: Andreolla, Ana Paula; Erpen, Luana Marina Scheer; Frandoloso, Rafael; Kreutz, Luiz Carlos.
Título: Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus
Fonte: Braz. j. microbiol;49(supl.1):68-75, 2018. tab, graf.
Idioma: en.
Projeto: CAPES; . SDECT; . CNPq.
Resumo: Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736 samples) and beef cattle (451 samples) with unknown status to BLV. We found that 31.1% (229/736) and 9.5% (43/451) of samples amongst dairy and beef cattle, respectively, had IgGs to BLV. The rate of agreement with a commercial competitive ELISA was 84.3% with a κ value of 0.68. Thus, our BLVp24r iELISA is suitable to detect BLV infected animals and should be a useful tool to control BLV infection in cattle.
Descritores: Ensaio de Imunoadsorção Enzimática/métodos
Testes Sorológicos/métodos
Leucose Enzoótica Bovina/diagnóstico
Vírus da Leucemia Bovina/imunologia
Proteínas do Capsídeo/imunologia
Anticorpos Antivirais/sangue
-Proteínas Recombinantes/análise
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Ensaio de Imunoadsorção Enzimática/instrumentação
Sensibilidade e Especificidade
Leucose Enzoótica Bovina/sangue
Leucose Enzoótica Bovina/virologia
Vírus da Leucemia Bovina/isolamento & purificação
Vírus da Leucemia Bovina/genética
Proteínas do Capsídeo/análise
Proteínas do Capsídeo/genética
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  7 / 5564 LILACS  
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Langoni, Hélio
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Id: biblio-974314
Autor: Oliveira, Pollyanne Raysa Fernandes de; Soares, Larice Bruna Ferreira; Borges, Jonas de Melo; Barrosa, Noelle de Castro; Langoni, Hélio; Brandespim, Daniel Friguglietti; Pinheiro Junior, José Wilton; Mota, Rinaldo Aparecido.
Título: Occurrence of serological reactions for serogroup Sejroe (CTG and Prajtino) in female buffalo in the state of Pernambuco, Brazil
Fonte: Braz. j. microbiol;49(4):795-800, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7-37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.
Descritores: Búfalos/microbiologia
Doenças dos Bovinos/sangue
Leptospira/imunologia
Leptospirose/veterinária
Anticorpos Antibacterianos/sangue
-Brasil
Testes de Aglutinação
Búfalos/imunologia
Doenças dos Bovinos/imunologia
Doenças dos Bovinos/microbiologia
Sorogrupo
Leptospira/isolamento & purificação
Leptospira/genética
Leptospirose/imunologia
Leptospirose/microbiologia
Leptospirose/sangue
Anticorpos Antibacterianos/imunologia
Limites: Animais
Feminino
Bovinos
Responsável: BR1.1 - BIREME


  8 / 5564 LILACS  
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Id: biblio-974303
Autor: Variane, Augusto Carlos Favaretto; Santos, Fabiane Cristina dos; Castro, Fausto Fernandes de; Barbosa-Tessmann, Ione Parra; Santos, Geraldo Tadeu dos; Pozza, Magali Soares dos Santos.
Título: The occurrence of aflatoxigenic Aspergillus spp. in dairy cattle feed in Southern Brazil
Fonte: Braz. j. microbiol;49(4):919-928, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Araucária Foundation, the Paraná State Science, Technology and Superior Studies Secretariat.
Resumo: ABSTRACT The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each sample was analyzed to check for the presence of aflatoxigenic Aspergillus. Water activity was observed to be lower in the concentrated feed samples. The pH was lower in the silage samples, indicating fermentation processes. Two silage samples and four concentrated feed samples were contaminated with Aspergillus spp. Seven isolates of Aspergillus spp. were obtained and their potential to produce aflatoxins was evaluated. Four of the isolates, two from the silage samples and two from the concentrated feed samples, produced the aflatoxins B1, B2, G1, and G2 in culture media. These isolates were identified as Aspergillus parasiticus and Aspergillus nomius. The presence of aflatoxigenic isolates of Aspergillus spp. in silage and concentrated feed samples is a matter of concern, because of the risk of aflatoxin production and contamination of the animal feed.
Descritores: Aspergillus/isolamento & purificação
Contaminação de Alimentos/análise
Aflatoxinas/metabolismo
Ração Animal/microbiologia
-Aspergillus/classificação
Aspergillus/genética
Aspergillus/metabolismo
Silagem/classificação
Silagem/microbiologia
Brasil
Ração Animal/análise
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


  9 / 5564 LILACS  
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Id: biblio-974302
Autor: Barreiro, Juliana R; Gonçalves, Juliano L; Grenfell, Rafaella; Leite, Renata F; Juliano, Luiz; Santos, Marcos V.
Título: Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk
Fonte: Braz. j. microbiol;49(4):801-807, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP; . Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University.
Resumo: ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to total bacterial count by flow cytometry and for a preparation protocol for bacterial ribosomal protein extraction followed by MALDI-TOF MS analysis. The direct MALDI-TOF MS identification method compared to microbiological culture correctly identified isolates of coagulase-negative Staphylococci (27.2%), Streptococcus agalactiae (21.8%), Staphylococcus aureus (14.2%), and Streptococcus uberis (5.2%). The pre-incubation protocol of milk samples, associated to the direct identification method by MALDI-TOF MS, did not increase the identification at species level (score >2.0) of pathogens causing subclinical mastitis in comparison to the method without previous incubation.
Descritores: Staphylococcus/isolamento & purificação
Streptococcus/isolamento & purificação
Técnicas de Tipagem Bacteriana/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Leite/microbiologia
Mastite Bovina/microbiologia
-Staphylococcus/genética
Staphylococcus/química
Streptococcus/genética
Streptococcus/química
Leite/química
Mastite Bovina/fisiopatologia
Limites: Animais
Feminino
Lactente
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-974292
Autor: Caldas, Lúcio Ayres; Freitas, Tânia Rosária Pereira; Azevedo, Renata Campos; de Souza, Wanderley.
Título: Prostaglandin A1 inhibits the replication of bovine viral diarrhea virus
Fonte: Braz. j. microbiol;49(4):785-789, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Bovine viral diarrhea virus can cause acute disease in livestock, leading to economic losses. We show that Prostaglandin A1 inhibits bovine viral diarrhea virus replication in Madin-Darby bovine kidney cells (94% inhibition using 5 µg/mL). Light and electron microscopy of infected cells shows that Prostaglandin A1 also prevents virus-induced vacuolization, but at higher concentrations (10 µg/mL).
Descritores: Antivirais/farmacologia
Prostaglandinas A/farmacologia
Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia
Vírus da Diarreia Viral Bovina/efeitos dos fármacos
-Antivirais/análise
Prostaglandinas A/análise
Replicação Viral/efeitos dos fármacos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/tratamento farmacológico
Linhagem Celular
Vírus da Diarreia Viral Bovina/fisiologia
Vírus da Diarreia Viral Bovina/genética
Diarreia
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME



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