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Id: biblio-1254696
Autor: Li, Feng; Xie, Yingjie; Gao, Xiang; Shan, Mingxu; Sun, Changchao; Dong Niu, Yan; Shan, Anshan.
Título: Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production
Fonte: Electron. j. biotechnol;48:29-35, nov. 2020. ilus, tab, graf.
Idioma: en.
Projeto: Open Project of Northeastern Science Inspection Station and China Ministry of Agriculture Key; . Laboratory of Animal Pathogen Biology; . China Agriculture Research System.
Resumo: BACKGROUND: Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production. RESULTS: In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively. CONCLUSIONS: Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.
Descritores: Porco Miniatura
Bactérias/enzimologia
Celulase/biossíntese
-Bacillus
Fibras na Dieta
Probióticos
Digestão
Fezes
Ração Animal
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-777320
Autor: Marchini, Giovannni Scala; Júniori, Italo D. Fioravanti; Horta, Leonardo V; Torricelli, Fabio C. M; Mitre, Anuar Ibrahim; Arap, Marco Antonio.
Título: Specific training for LESS surgery results from a prospective study in the animal model
Fonte: Int. braz. j. urol;42(1):90-95, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective to prospectively evaluate the ability of post-graduate students enrolled in a laparoscopy program of the Institute for Teaching and Research to complete single port total nephrectomies. Materials and Methods 15 post-graduate students were enrolled in the study, which was performed using the SILStm port system for single-port procedures. All participants were already proficient in total nephrectomies in animal models and performed a left followed by a right nephrectomy. Analyzed data comprised incision size, complications, and the time taken to complete each part of the procedure. Statistical significance was set at p<0.05. Results All students successfully finished the procedure using the single-port system. A total of 30 nephrectomies were analyzed. Mean incision size was 3.61 cm, mean time to trocar insertion was 9.61 min and to dissect the renal hilum was 25.3 min. Mean time to dissect the kidney was 5.18 min and to complete the whole procedure was 39.4 min. Total renal hilum and operative time was 45.8% (p<0.001) and 38% (p=0.001) faster in the second procedure, respectively. Complications included 3 renal vein lesions, 2 kidney lacerations and 1 lesion of a lumbar artery. All were immediately identified and corrected laparoscopically through the single-port system, except for one renal vein lesion, which required the introduction an auxiliary laparoscopic port. Conclusion Laparoscopic single-port nephrectomy in the experimental animal model is a feasible but relatively difficult procedure for those with intermediate laparoscopic experience. Intraoperative complications might be successfully treated with the single-port system. Training aids reducing surgical time and improves outcomes.
Descritores: Laparoscopia/educação
Modelos Animais
Educação Médica/métodos
Nefrectomia/educação
-Estudantes de Medicina
Suínos
Porco Miniatura
Análise e Desempenho de Tarefas
Estudos Prospectivos
Reprodutibilidade dos Testes
Competência Clínica
Laparoscopia/métodos
Duração da Cirurgia
Complicações Intraoperatórias
Rim/cirurgia
Nefrectomia/métodos
Limites: Humanos
Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1137304
Autor: Yin, Shijie; Feng, Zhiqiang; Mo, Ansheng; Ding, Yi; Wu, Jun.
Título: Effect of shenfu injection on isolated empty beating hearts from miniature pigs
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);35(4):484-489, July-Aug. 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Natural Science Foundation of Guangxi.
Resumo: Abstract Objective: To investigate the effect of Shenfu (SF) injection on donor heart preservation. Methods: Twelve pigs were randomly divided into SF group (n=6) and control group (n=6). After eight hours of perfusion, the differences in hemoglobin, the expression of Bcl-2 and BAX, and changes in the myocardial ultrastructure were compared to illustrate the effects of SF injection in heart preservation. Results: The differences in free hemoglobin between the SF group and the control group were statistically significant (P=0.001), and there was significant interaction of groups with times (P=0.019), but the perfusion time may not be associated with the hemoglobin concentration (P=0.616). According to Western blotting analysis, the expression of Bcl-2 was higher in the SF group than in the control group, while the expression of BAX was not different between the two groups. As to ultrastructural changes, both groups exhibited mitochondrial swelling and myofilament lysis, but the degree of damage in the SF group was smaller. Conclusion: Our study suggests that the application of SF injection for heart preservation may protect against cardiomyocytes and erythrocytes apoptosis, and Bcl-2 protein may play a role in these physiological processes.
Descritores: Medicamentos de Ervas Chinesas
Transplante de Coração
-Suínos
Porco Miniatura
Doadores de Tecidos
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-886279
Autor: Oliveira, André Lacerda de Abreu; Scheffer, Jussara Peters; Markoski, Melissa; Koche, Andreia; Balbinot, Alexsandra; Antunes, Fernanda; Kalil, Renato.
Título: Vascular endothelial growth factor association with angiopoietin 1 promotes improvement in ventricular function after ischemic cardiomyopathy induced in mini pigs
Fonte: Acta cir. bras;33(4):386-395, Apr. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the safety and clinical, hemodynamic and tissue improvement ability in mini pigs undergoing cell and gene therapy for the treatment of acute myocardial infarction. Methods: Thirty-two mini pigs Br1 lineage, 12 months old, undergoing induction of acute myocardial infarction by occlusion of the diagonal branch of the paraconal coronary. They were divided into 4 groups: one control group and 3 treatment groups (cell therapy and gene cell therapy). Echocardiography reviews were performed on three occasions and histopathological analysis was performed after 4 weeks. Analysis of variance (ANOVA), Tukey and Wilcoxon tests, were performed. Results: Association of vascular endothelial growth factor (VEGF) with angiopoietin1 (Ang1) presented satisfactory results in the improvement of ventricular function following ischemic cardiomyopathy in mini pigs when compared to the results of the other treated groups. Conclusion: The therapy with VEGF and the combination of VEGF with Ang1, promoted recovered function of the myocardium, characterized by reduced akinetic area and induction of neovascularization.
Descritores: Terapia Genética/métodos
Função Ventricular/fisiologia
Angiopoietina-1/uso terapêutico
Fator A de Crescimento do Endotélio Vascular/uso terapêutico
Terapia Baseada em Transplante de Células e Tecidos/métodos
Infarto do Miocárdio/terapia
-Suínos
Porco Miniatura
Cicatrização
Ecocardiografia
Reprodutibilidade dos Testes
Resultado do Tratamento
Neovascularização Fisiológica
Modelos Animais de Doenças
Hemodinâmica/fisiologia
Infarto do Miocárdio/fisiopatologia
Infarto do Miocárdio/patologia
Necrose
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-886185
Autor: Arruda, Eduardo Gustavo Pires de; Munhoz, Alexandre Mendonça; Matsumoto, Walter; Ueda, Thiago; Coudry, Renata de Almeida; Gemperli, Rolf.
Título: Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs
Fonte: Acta cir. bras;32(11):891-902, Nov. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Purpose: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. Methods: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. Results: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. Conclusion: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.
Descritores: Transplante Autólogo/métodos
Tecido Adiposo/transplante
Procedimentos Cirúrgicos Reconstrutivos/métodos
Modelos Animais de Doenças
-Pressão
Suínos
Porco Miniatura
Transplante Autólogo/normas
Imuno-Histoquímica
Estudos de Viabilidade
Fator de Necrose Tumoral alfa
Molécula-1 de Adesão Celular Endotelial a Plaquetas/análise
Procedimentos Cirúrgicos Reconstrutivos/normas
Perilipinas/análise
Sobrevivência de Enxerto
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-997084
Autor: Silveira, Renato Luiz; Cruz, Ana Claudia de Menezes; Medina, Raphael Mansur; Câmara Filho, Jurandyr de Abreu; Abílio, Edmundo Jorge; Carvalho, Eulógio Carlos Queiroz de.
Título: Lesões pré-ulcerativas gastroesofágicas em miniporcos / Gastroesophageal ulcer research in mini pigs
Fonte: Rev. bras. ciênc. vet;22(3-4):160-164, jul.-dez.2015. il..
Idioma: pt.
Resumo: A úlcera gastroesofágica figura como uma das principais causas de morte súbita de suínos em fase de reprodução. O objetivo deste trabalho foi avaliar os achados anatomopatológicos das lesões gástricas subclínicas em miniporcos. Quarenta animais, com peso médio de 39kg e idade de 17 meses, foram mantidos em biotério, em condições de temperatura e umidade constantes, alimentados duas vezes ao dia, sendo depois eutanasiados e necropsiados. Os estômagos foram colhidos e avaliados. Macroscopicamente, 29 animais apresentaram alterações na região aglandular, sendo que 37,5% foram classificadas no grau 1; 22,5%, no grau 2; e 12,5%, no grau 3. Microscopicamente, 29 (72,5%) miniporcos apresentaram paraqueratose. Destes, 35% tinham a forma moderada, 25% a discreta e 12,5% a acentuada. As lesões glandulares foram mais extensas na região cárdica, seguida da antral e fúndica. Trinta e seis (90%) animais apresentaram lesão em pelo menos uma das três regiões. Em 21 (52,5%) mini- porcos observou-se hiperatividade mucípara com catarro; em 17 (42,5%), aumento da atividade mucípara; em dez (25%), erosão. Os achados revelaram alta prevalência de lesões pré-ulcerativas nos estômagos dos miniporcos. A presença destas relacionou-se à gravidade macroscópica: quanto mais graves, mais intensa a paraqueratose.

Gastro-esophageal ulcers are one of the principal causes of reproduction pigs sudden death. This work aimed to evaluate the pathological findings of subclinical gastric lesions naturally occurring in mini pigs. Forty mini pigs with a mean weight of 39.0 kg and 17 months of age kept in animal facility, under constant temperature and humidity, and fed twice a day, were euthanized and necropsied. Their stomachs were collected and evaluated. Macroscopic lesions in aglandular portion were observed in 29 animals, and 37.5% of them were classified as grade 1, 22.5% as grade 2, and 12.5% as grade 3. In 29 (72.5%) animals parakeratosis was microscopically seen: 35% had the moderate manner, 25% discreet and 12.5% severe one. Glandular lesions were more extensive in the cardia region, followed by antral and fundic. Thirty-six (90%) animals had lesions in at least one of three regions. In 21 (52.5%) mini pigs, it was observed mucipara hyperactivity with phlegm; in 17 (42.5%), increased mucipara activity; and in ten (25%), erosion. Findings revealed high prevalence of pre-ulcerative lesions in the stomachs of mini pigs. The presence of these was related to the macroscopic gravity: the more serious, more intense was the parakeratosis.
Descritores: Úlcera Gástrica
Porco Miniatura
-Estômago
Suínos
Limites: Animais
Responsável: BR409.1 - Biblioteca


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Id: lil-771882
Autor: Barros, R; Rodrigues, A. C. L.; Guberman, U. C.; Gandolfi, M. G.; Merlini, N. B.; Teixeira, C. R.; Padovani, C. R.; Brancalion, B. B.; Ranzani, J. J. T.; Brandão, C. V. S..
Título: Mensurações do bulbo ocular e cálculo do poder dióptrico da lente intraocular em miniporcos / Ocular bulb measurements and diopter power calculation of the lens in minipigs
Fonte: Arq. bras. med. vet. zootec;68(1):141-146, jan.-fev. 2016. tab.
Idioma: pt.
Resumo: Objetivou-se com este trabalho determinar o poder dióptrico da lente intraocular (LIO) em miniporco e as dimensões do bulbo do olho. Foram utilizados 17 miniporcos, sadios, adultos, machos e fêmeas, com peso médio de 70kg. Em todos os olhos foram realizadas a ultrassonografia modo A, a ceratometria e a medida da distância limbo a limbo. O cálculo do poder dióptrico da LIO foi obtido utilizando-se as fórmulas Haigis, Hoffer Q, SRK/T, Holladay I e Holladay II e o software Holladay IOL Consultant(r). Na comparação entre o sexo e a lateralidade do olho, não houve diferença nas variáveis biométricas e poder da LIO. A aplicação das fórmulas (Haigis, Holladay II, Holladay I, SRK/T e Hoffer Q) possibilitou o cálculo do poder da LIO. A Holladay II, fórmula que melhor individualiza o bulbo do olho do miniporco, estima valor dióptrico ao redor de 41 D. Os miniporcos têm potencial como modelo experimental em oftalmologia, relacionado ao seu menor porte e à facilidade no manejo, especialmente em experimentos de longa duração.

The aim of this study was to determine the refractive power of intraocular lens (IOL) of mini pigs and the dimensions of the eyeball. A total of 17 (34 eyes) healthy, adult, males and female animals, with average weight of 70kg were used. For every eye, A-mode ultrasound, keratometry and the measurement of limbo-to-limbo distance were conducted, all variables for calculating the refractive power of the IOL. The value was obtained using different formulas and Holladay IOL Consultant(r) Software. Additionally, the ocular measurements were compared per sex, laterality of the eye and the different formulas used in this study (Haigis, Hoffer Q, SRK / T, Holladay I and Holladay II). In the comparison between sex and laterality of the eye, there was no difference in biometric variables and power of the IOL. The application of the employed formulas (Haigis, Holladay II, Holladay I, SRK / T and Hoffer Q) allowed the IOL power calculation for this specie, and the observed value ranged between 39.58±2.15 and 46.60±2.81 diopters. Mini pigs play an important and growing role as an experimental model for study and practice of ophthalmic procedures, specially related to their smaller size and easy management in long-term experiments.
Descritores: Experimentação Animal
Oftalmologia
Porco Miniatura/anatomia & histologia
-Biometria
Lentes Intraoculares/veterinária
Modelos Animais
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-719920
Autor: Zanicotti, Roberta Targa Stramandinoli.
Título: Avaliação da interface osso- implante em mandíbula de miniporcos irradiados e o uso de células-tronco mesenquimais associadas ao plasma rico em plaquetas na osseointegração / Evaluation of the bone-implant contact in the mandible of irradiated minipigs and the use of mesenchymal stem cells associated with platelet-rich plasma on osseointegration.
Fonte: São Paulo; s.n; 2013. [121] p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: Introdução: Pacientes com câncer na região de cabeça e pescoço normalmente são tratados por combinação de cirurgia, radioterapia (RT) e quimioterapia. Em muitos casos, a reabilitação oral com próteses implanto-suportadas representa a melhor opção para uma recuperação funcional adequada. Entretanto, em pacientes irradiados procedimentos como exodontias e instalações de implantes dentários são fatores de risco ao desenvolvimento de osteorradionecrose. Diversos estudos experimentais têm demonstrado que o uso de células-tronco mesenquimais (CTMs) associadas a fatores de crescimento como plasma rico em plaquetas (PRP) proporciona melhora no reparo ósseo e na osseointegração, podendo ser considerada uma alternativa viável para defeitos ósseos ou injúria. Objetivos: Isolar e caracterizar as CTMs da medula óssea (MO) de miniporcos brasileiros (Minipigs BR-1), avaliar a interferência da RT e o efeito da associação de CTMs-MO+PRP no processo de osseointegração de implantes instalados em alvéolos frescos em mandíbulas de miniporcos, por análise histológica e histomorfométrica da interface osso-implante. Métodos: CTMs-MO de 12 miniporcos adultos machos foram isoladas da crista ilíaca. Após 21 dias de cultura, o potencial de diferenciação celular foi avaliado por meio de coloração e RT-PCR. O perfil imunofenotípico foi caracterizado por citometria de fluxo. Os animais foram divididos em três grupos: Grupo A (grupo controle, sem RT), Grupo B (implantes instalados 15 dias antes da RT) e Grupo C (implantes instalados três meses após a RT). A dose total de radiação para cada lado da mandíbula foi de 24 Gy, divididos em três doses de 8 Gy com intervalo de 7 dias entre as doses, a qual equivale biologicamente a aproximadamente 56 Gy, com 28 exposições de 2 Gy cada. Quatro implantes de titânio foram instalados nos alvéolos frescos, imediatamente após as extrações dos terceiro e quarto pré-molares, totalizando 48 implantes controles e 48 experimentais (uso de CTM-MO+PRP)...

Introduction: Patients with head and neck cancer are usually treated by a combination of surgery, radiotherapy (RT) and chemotherapy. In many cases, oral rehabilitation with implant-supported prostheses is the best option for a proper functional recover. However, in irradiated patients procedures as dental extractions and implants are risk factors for developing osteoradionecrosis. Several experimental studies have shown that the use of mesenchymal stem cells (MSCs) associated with growth factors such as platelet-rich plasma (PRP) provides improvement in bone regeneration and osseointegration, being considered an alternative to bone defects or injury. Objectives: To isolate and characterize MSCs from bone marrow (BM) of Brazilian minipigs (Minipigs BR-1), to evaluate the effect of RT and of BM-MSCs+PRP in the osseointegration of implants placed in fresh sockets, by histological and histomorphometric analysis of the bone-implant interface. Methods: BM-MSCs from 12 adult male minipigs were isolated from the iliac crest. After 21 days of culture, cell differentiation potential was assessed by staining and RT-PCR. The immunophenotypic profile was characterized by flow cytometry. The animals were divided into three groups: Group A (control group, no RT), Group B (implants placement 15 days before RT) and Group C (implants placement three months after RT). The total radiation dose for each side of the mandible was 24 Gy, divided into 3 doses of 8 Gy with a 7 dayinterval for each dose, which is biologically equivalent to approximately 56 Gy, with 28 sections of 2 Gy each. Four titanium implants were installed in the alveoli fresh, immediately after the extraction of the third and fourth premolars of each hemimandible, totalling 48 implants on the control side and 48 on the experimental side (using BM-MSCs+PRP). The animals were euthanized 90 days post-implantation....
Descritores: Implantes Dentários
Osseointegração
Plasma Rico em Plaquetas
Radioterapia
Células-Tronco
Porco Miniatura
Limites: Animais
Masculino
Adulto
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


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Id: lil-719310
Autor: Tian, Y.G.; Yue, M.; Gu, Y.; Gu, W.W.; Wang, Y.J..
Título: Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(9):753-758, 09/2014. tab.
Idioma: en.
Projeto: This research was supported by the Guangdong Natural Science Foundation; . ; China Postdoctoral Science Foundation; . ; the National High Technology Research and Development Program of China (863 Program: #2011AA100304); Science and Technology Planning Project; . of Guangzhou City, Guangdong Province, China; Conditions for scientific research project; . of Guangdong Province, China; Key Program for International S&T Cooperation Projects of China; . ; Torch Program; . , and Development Foundation of Special High-tech Zone.
Resumo: Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen circumference (AC) - three SNP loci in BMs and four SNP loci in TBs significantly affected growth traits. Three SNP sites in BMs and four SNP sites in TBs significantly affected growth traits. SNPs located in the GH gene fragment significantly affected BL and CC at locus 12 and BL at locus 45 in BMs, and also BW, WH, CC, and AC at locus 45 and WH and CC at locus 93 in TBs. One SNP at locus 85 in the BM GHR gene fragment significantly affected all growth traits. All indices were significantly reduced with a mixture of alleles at locus 85. These results provide more information regarding the genetic background of these minipig species and indicate useful selection markers for pig breeding programs.
Descritores: Hormônio do Crescimento/genética
Fator de Crescimento Insulin-Like I/genética
Polimorfismo de Nucleotídeo Único/fisiologia
Receptores da Somatotropina/genética
Porco Miniatura/genética
-Alelos
Tamanho Corporal
DNA
Nanismo/genética
Loci Gênicos
Genótipo
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Suínos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-711715
Autor: STRAMANDINOLI-ZANICOTTI, Roberta Targa; CARVALHO, André Lopes; REBELATTO, Carmen Lúcia Kuniyoshi; SASSI, Laurindo Moacir; TORRES, Maria Fernanda; SENEGAGLIA, Alexandra Cristina; BOLDRINILEITE, Lidiane Maria; CORREA-DOMINGUEZ, Alejandro; KULIGOVSKY, Crisciele; BROFMAN, Paulo Roberto Slud.
Título: Brazilian minipig as a large-animal model for basic research and stem cell-based tissue engineering. Characterization and in vitro differentiation of bone marrow-derived mesenchymal stem cells
Fonte: J. appl. oral sci;22(3):218-227, May-Jun/2014. tab, graf.
Idioma: en.
Resumo: Stem cell-based regenerative medicine is one of the most intensively researched medical issues. Pre-clinical studies in a large-animal model, especially in swine or miniature pigs, are highly relevant to human applications. Mesenchymal stem cells (MSCs) have been isolated and expanded from different sources. Objective: This study aimed at isolating and characterizing, for the first time, bone marrow-derived MSCs (BM-MSCs) from a Brazilian minipig (BR1). Also, this aimed to validate a new large-animal model for stem cell-based tissue engineering. Material and Methods: Bone marrow (BM) was aspirated from the posterior iliac crest of twelve adult male BR1 under general anesthesia. MSCs were selected by plastic-adherence as originally described by Friedenstein. Cell morphology, surface marker expression, and cellular differentiation were examined. The immunophenotypic profile was determined by flow cytometry. The differentiation potential was assessed by cytological staining and by RT-PCR. Results: MSCs were present in all minipig BM samples. These cells showed fibroblastic morphology and were positive for the surface markers CD90 (88.6%), CD29 (89.8%), CD44 (86.9%) and negative for CD34 (1.61%), CD45 (1.83%), CD14 (1.77%) and MHC-II (2.69%). MSCs were differentiated into adipocytes, osteoblasts, and chondroblasts as demonstrated by the presence of lipidic-rich vacuoles, the mineralized extracellular matrix, and the great presence of glycosaminoglycans, respectively. The higher gene expression of adipocyte fatty-acid binding protein (AP2), alkaline phosphatase (ALP) and collagen type 2 (COLII) also confirmed the trilineage differentiation (p<0.001, p<0.001, p=0.031; respectively). Conclusions: The isolation, cultivation, and differentiation of BM-MSCs from BR1 makes this animal eligible as a useful large-animal model for stem cell-based studies in Brazil. .
Descritores: Células da Medula Óssea/citologia
Modelos Animais
Células-Tronco Mesenquimais/citologia
Porco Miniatura
Engenharia Tecidual/métodos
-Antígenos CD/análise
Brasil
Técnicas de Cultura de Células
Diferenciação Celular
Sobrevivência Celular
Células Cultivadas
Citometria de Fluxo
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Suínos
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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