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Texto completo SciELO Brasil
Buzalaf, Marilia Afonso Rabelo
Texto completo
Id: lil-787547
Autor: KHAN, Zohaib Nisar; LEITE, Aline de Lima; CHARONE, Senda; SABINO, Isabela Tomazini; MARTINI, Tatiana; PEREIRA, Heloísa Aparecida Barbosa da Silva; OLIVEIRA, Rodrigo Cardoso; BUZALAF, Marília Afonso Rabelo.
Título: Liver proteome of mice with different genetic susceptibilities to the effects of fluoride
Fonte: J. appl. oral sci;24(3):250-257tab, graf.
Idioma: en.
Resumo: ABSTRACT A/J and 129P3/J mice strains have been widely studied over the last few years because they respond quite differently to fluoride (F) exposure. 129P3/J mice are remarkably resistant to the development of dental fluorosis, despite excreting less F in urine and having higher circulating F levels. These two strains also present different characteristics regardless of F exposure. Objective In this study, we investigated the differential pattern of protein expression in the liver of these mice to provide insights on why they have different responses to F. Material and Methods Weanling male A/J and 129P3/J mice (n=10 from each strain) were pared and housed in metabolic cages with ad libitum access to low-F food and deionized water for 42 days. Liver proteome profiles were examined using nLC-MS/MS. Protein function was classified by GO biological process (Cluego v2.0.7 + Clupedia v1.0.8) and protein-protein interaction network was constructed (PSICQUIC, Cytoscape). Results Most proteins with fold change were increased in A/J mice. The functional category with the highest percentage of altered genes was oxidation-reduction process (20%). Subnetwork analysis revealed that proteins with fold change interacted with Disks large homolog 4 and Calcium-activated potassium channel subunit alpha-1. A/J mice had an increase in proteins related to energy flux and oxidative stress. Conclusion This could be a possible explanation for the high susceptibility of these mice to the effects of F, since the exposure also induces oxidative stress.
Descritores: Fluoretos/toxicidade
Fluorose Dentária/genética
Predisposição Genética para Doença
Fígado/efeitos dos fármacos
Proteoma/efeitos dos fármacos
Expressão Gênica
Espectrometria de Massas/métodos
Camundongos da Linhagem 129
Camundongos Endogâmicos A
Estresse Oxidativo/efeitos dos fármacos
Domínios e Motivos de Interação entre Proteínas
Proteínas/efeitos dos fármacos
Valores de Referência
Fatores de Tempo
Limites: Animais
Responsável: BR1.1 - BIREME

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