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Id: lil-788966
Autor: Azizoglu, Ugur; Ayvaz, Abdurrahman; Yılmaz, Semih; Karabörklü, Salih; Temizgul, Rıdvan.
Título: Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Fonte: Braz. j. microbiol;47(3):597-602, July-Sept. 2016. graf.
Idioma: en.
Projeto: Erciyes University; . Bilim, Sanayi ve Teknoloji Bakanlığı.
Resumo: ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
Descritores: Bacillus thuringiensis/fisiologia
Proteínas de Bactérias/genética
Expressão Gênica
Controle de Insetos
Endotoxinas/genética
Proteínas Hemolisinas/genética
-Bacillus thuringiensis/ultraestrutura
Proteínas de Bactérias/metabolismo
Proteínas de Bactérias/toxicidade
Controle de Insetos/métodos
Clonagem Molecular
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Proteínas Hemolisinas/metabolismo
Proteínas Hemolisinas/toxicidade
Inseticidas
Larva
Mariposas/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-780820
Autor: Ramya, Shanivarsanthe Leelesh; Venkatesan, Thiruvengadam; Srinivasa Murthy, Kottilingam; Jalali, Sushil Kumar; Verghese, Abraham.
Título: Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb degradation
Fonte: Braz. j. microbiol;47(2):327-336, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates, gammaproteobacteria was the most abundant (76%), followed by bacilli (15.4%). Molecular characterization placed adult gut bacterial strains into three major classes based on abundance: gammaproteobacteria (66%), bacilli (16.7%) and flavobacteria (16.7%). Esterase activity from 19 gut bacterial isolates ranged from 0.072 to 2.32 µmol/min/mg protein. Esterase bands were observed in 15 bacterial strains and the banding pattern differed in Bacillus cereus – KC985225 and Pantoea agglomerans – KC985229. The bands were characterized as carboxylesterase with profenofos used as an inhibitor. Minimal media study showed that B. cereus degraded indoxacarb up to 20%, so it could use indoxacarb for metabolism and growth. Furthermore, esterase activity was greater with minimal media than control media: 1.87 versus 0.26 µmol/min/mg protein. Apart from the insect esterases, bacterial carboxylesterase may aid in the degradation of insecticides in DBM.
Descritores: Oxazinas/metabolismo
Bactérias/enzimologia
Carboxilesterase/metabolismo
Esterases/metabolismo
Microbioma Gastrointestinal
Inseticidas/metabolismo
Mariposas/microbiologia
-Filogenia
Bactérias/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Trato Gastrointestinal/microbiologia
Carboxilesterase/genética
Esterases/genética
Índia
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-1024603
Autor: Souza, Thiago Sampaio de; Aguiar-Menezes, Elen de Lima; Resende, André Luis Santos; Pereira, Thalles Platiny Lavinscky; Gazal, Vinícius Siqueira.
Título: Megaselia Rondani (Diptera: Phoridae) larvae as a Sphingidae (Lepidoptera) parasitoid / Larvas de Megaselia Rondani (Diptera: Phoridae) como parasitoide de Sphingidae (Lepidoptera)
Fonte: Arq. Inst. Biol;86:e0812018, 2019. ilus.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Detritivoria and parasitism are dietary habits already registered for the phorids of the genus Megaselia Rondani. The results of this study confirm the parasitic habit of Megaselia larvae. This is the first Pachylia ficus (Linnaeus, 1758) (Lepidoptera: Sphingidae) adult record as a host of Megaselia scalaris (Loew, 1866) and Megaselia sp. (Diptera: Phoridae).(AU)

Detritivoria e parasitismo são hábitos alimentares já registrados para os forídeos do gênero Megaselia Rondani. Os resultados do presente estudo confirmam o hábito parasitário das larvas de Megaselia. Este é o primeiro registro de adulto de Pachylia ficus (Linnaeus, 1758) (Lepidoptera: Sphingidae) como hospedeiro de Megaselia scalaris (Loew, 1866) e Megaselia sp. (Diptera: Phoridae).(AU)
Descritores: Doenças Parasitárias
Dípteros
-Larva
Mariposas
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-838215
Autor: Casado Verrier, Esther; Carro Rodríguez, Miguel A; Cancho, María de la Parte; Piñeiro Pérez, Roi.
Título: Reacción sistémica tras la ingesta de una oruga procesionaria del pino: ¿Manejo conservador? / Systemic reaction after pine processionary caterpillar ingestion: Conservative management?
Fonte: Arch. argent. pediatr;114(3):e151-e154, jun. 2016. ilus.
Idioma: es.
Resumo: La oruga procesionaria del pino, forma larvaria de la mariposa nocturna Thaumetopoea pityocampa, es una de las principales plagas forestales del sur de Europa. Con frecuencia, estas orugas producen reacciones locales en humanos debidas a los pelillos urticantes microscópicos que las recubren. La mayoría de los síntomas ocasionados son cutáneos, como urticaria aguda, y son mucho más infrecuentes las reacciones sistémicas. Se presenta un caso de reacción sistémica, con edema orofacial, babeo y urticaria tras la ingesta de una oruga procesionaria del pino en un lactante de 11 meses, en el cual el tratamiento farmacológico fue suficiente para la resolución de los síntomas. No tuvo, en ningún momento, compromiso de la vía aérea ni otros datos de anafilaxia, por lo que se desestimaron actuaciones más agresivas, como intubación orotraqueal, endoscopía o laringoscopía.

The larval form of the moth Thaumetopoea pityocampa, known as pine processionary caterpillar, is one of the main forest pests in southern Europe. Often, these caterpillars cause local reactions in humans, due to their microscopic stinging hairs. Most symptoms affect skin, in form of acute urticaria. Systemic reactions are rare. An eleven month old infant with a systemic reaction after ingestion of a pine processionary caterpillar is presented. The boy reached the Emergency Room with orofacial edema, drooling and urticaria. Drug treatment was enough to resolve the symptoms. The patient did not present airway commitment, nor anaphylaxis data at any moment, so more aggressive actions, such as endotracheal intubation, endoscopy or laryngoscopy, were rejected.
Descritores: Urticária/terapia
Ingestão de Alimentos
Edema/terapia
Tratamento Conservador
Larva
Mariposas
-Urticária/etiologia
Edema/etiologia
Animais
Limites: Humanos
Masculino
Lactente
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-889152
Autor: Dhawan, Manish; Joshi, Neelam.
Título: Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN
Fonte: Braz. j. microbiol;48(3):522-529, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of fungal isolates were measured. Variability was observed both in the pattern of enzyme secretion and the level of enzyme activities among various fungal isolates. B. bassiana MTCC 4495 recorded the maximum mean chitinase (0.51 U/ml), protease (1.12 U/ml), and lipase activities (1.36 U/ml). The minimum mean chitinase and protease activities (0.37 and 0.91 U/ml, respectively) were recorded in B. bassiana MTCC 6291. The minimum mean lipase activity (1.04 U/ml) was recorded in B. bassiana NBAII-11. Our studies revealed B. bassiana MTCC 4495 as the most pathogenic isolate against P. brassicae, which also recorded maximum extracellular enzyme activities, suggesting the possible roles of extracellular enzymes in the pathogenicity of B. bassiana against P. brassicae.
Descritores: Beauveria/enzimologia
Beauveria/patogenicidade
Brassica/parasitologia
Quitinases/metabolismo
Proteínas Fúngicas/metabolismo
Mariposas/microbiologia
Controle Biológico de Vetores/métodos
Doenças das Plantas/parasitologia
-Beauveria/genética
Quitinases/genética
Proteínas Fúngicas/genética
Larva/microbiologia
Larva/fisiologia
Mariposas/fisiologia
Virulência
Limites: Animais
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-753178
Autor: Maggi, Silviane; Faulhaber, Gustavo Adolpho Moreira.
Título: Lonomia obliqua Walker (Lepidoptera: Saturniidae): hemostasis implications / Lonomia obliqua Walker (Lepidoptera: Saturniidae): implicações em hemostasia.
Fonte: Rev. Assoc. Med. Bras. (1992);61(3):263-268, May-Jun/2015.
Idioma: en.
Resumo: Summary In southern Brazil, since 1989, several cases of accidents produced by unwilling contact with the body of poisonous caterpillars of the moth species Lonomia obliqua Walker, 1855 (Lepidoptera: Saturniidae), were described. L. obliqua caterpillars have gregarious behavior and feed on leaves of host trees during the night, staying grouped in the trunk during the day, which favors the occurrence of accidents with the species. This caterpillar has the body covered with bristles that on contact with the skin of individuals, breaks and release their contents, inoculating the venom into the victim. The basic constitution of the venom is protein and its components produce physiological changes in the victim, which include disturbances in hemostasis. Hemorrhagic syndrome associated with consumption coagulopathy, intravascular hemolysis and acute renal failure are some of the possible clinical manifestations related to poisoning by L. obliqua. Specific laboratory tests for diagnosis of poisoning have not been described previously. The diagnosis of poisoning is made based on the patient's medical history, clinical manifestations, erythrocyte levels, and, primarily, parameters that evaluate blood coagulation. Treatment is performed with the use of supportive care and the administration of specific hyperimmune antivenom. Poisoning can be serious and even fatal.

Na região Sul do Brasil, a partir de 1989, foram descritos inúmeros casos de contato acidental com o corpo de lagartas venenosas da mariposa da espécie Lonomia obliqua Walker, 1855 (Lepidoptera: Saturniidae). As lagartas de L. obliqua possuem comportamento gregário e alimentam- -se de folhas das árvores hospedeiras durante a noite, permanecendo agrupadas no tronco durante o dia, o que favorece a ocorrência de acidentes. Essa lagarta possui o corpo recoberto por cerdas urticantes, que, ao contato com a pele dos indivíduos, se rompem e liberam seu conteúdo, inoculando o veneno na vítima. A constituição básica do veneno é proteica e seus componentes produzem modificações fisiológicas no acidentado, que incluem distúrbios na hemostasia. Síndrome hemorrágica associada a coagulopatia de consumo, hemólise intravascular e insuficiência renal aguda são algumas das manifestações clínicas possíveis relacionadas ao envenenamento por L. obliqua. Ainda não foram descritas avaliações laboratoriais específicas para diagnóstico do envenenamento, que é feito com base na anamnese do paciente, nas manifestações clínicas, nos índices hematimétricos e, principalmente, nos parâmetros que avaliam a coagulação sanguínea. A terapêutica é feita com a utilização de medidas de suporte e com a administração de um soro heterólogo hiperimune específico. Os envenenamentos podem ser graves e até mesmo fatais.
Descritores: Venenos de Artrópodes/envenenamento
Hemostasia
Lepidópteros
-Venenos de Artrópodes/metabolismo
Brasil
Coagulação Sanguínea/efeitos dos fármacos
Coagulação Intravascular Disseminada/etiologia
Hemorragia/induzido quimicamente
Mariposas
Serina Endopeptidases/metabolismo
Limites: Animais
Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-731227
Autor: Medeiros, Daniela Nasu Monteiro; Torres, Hélida Conceição Cavalcante; Troster, Eduarto Juan.
Título: Accident involving a 2-year-old child and Lonomia obliqua venom: clinical and coagulation abnormalities
Fonte: Rev. bras. hematol. hemoter;36(6):445-447, Nov-Dec/2014. tab, graf.
Idioma: en.
Resumo: Poisons of caterpillars have different effects on inflammatory and coagulation systems. This is a case report of a 2-year-old child that accidentally came in contact with several caterpillars of the species Lonomia obliqua. At first, the patient's exams presented abnormal coagulation and decreased fibrinogen, but the patient did not evolve to active bleeding or acute renal failure. The patient received antilonomic serum 15 h after the accident and the treatment was repeated after another 12 h due to persistent alterations shown by the coagulation exams. The venom of L. obliqua has several substances that act on the coagulation and inflammatory systems. The event is characterized by a hemorrhagic syndrome with decreases in fibrinogen. L. obliqua Stuart-factor activator (Losac) and L. obliqua prothrombin activator protease (Lopap) are components that act with procoagulatory effects. The pro-inflammatory action occurs due to metalloproteases, hyaluronidases and other substances with inflammatory activity. Studies on caterpillar venom can give new perspectives on the treatment of cancer and other diseases that cause dysfunction of the extra-cellular matrix...
Descritores: Acidentes
Anticoagulantes
Coagulação Sanguínea
Criança
Coagulação Intravascular Disseminada/complicações
Mariposas
Limites: Humanos
Masculino
Pré-Escolar
Tipo de Publ: Relatos de Casos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-724663
Autor: Haddad, Vidal; Lastória, Joel Carlos.
Título: Envenomation by caterpillars (erucism): proposal for simple pain relief treatment
Fonte: J. venom. anim. toxins incl. trop. dis;20, 04/02/2014.
Idioma: en.
Descritores: Envenenamento
Toxicologia
-Larva/classificação
Mariposas/classificação
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-709812
Autor: Araujo, Laura M.L.; Rosário Filho, Nelson A.; Riedi, Carlos A..
Título: Respiratory allergy to moth: the importance of sensitization to Bombyx mori in children with asthma and rhinitis / Alergia respiratória à mariposa: importância da sensibilização à Bombyx mori em crianças com asma e rinite
Fonte: J. pediatr. (Rio J.);90(2):176-180, Mar-Apr/2014. tab.
Idioma: en.
Resumo: OBJECTIVE: this study aimed to prepare a silkworm moth (Bombyx mori) antigenic extract and to perform skin prick tests with this extract in patients with allergic respiratory diseases; to evaluate serum specific immunoglobulin E (IgE) to Bombyx mori using ImmunoCAP(r) system and to report the frequency of positivity between the two methods and with clinical data. METHODS: this was a cross-sectional study with 99 children and adolescents diagnosed with asthma and/or allergic rhinitis, who had skin reactivity to at least one of the six aeroallergens tested. Clinical data were evaluated: skin prick tests with Bombyx mori in-house extract, and total and specific IgE analysis using ImmunoCAP(r) were performed. RESULTS: the frequency of Bombyx mori specific IgE was found to be 52.5% and 60% using the skin prick test and ImmunoCAP(r), respectively. An association between a positive skin test for Bombyx mori and the presence of allergic rhinitis, atopic dermatitis, and urticaria was observed, but the same was not true for asthma or allergic conjunctivitis. There was no relation with the severity of asthma or rhinitis symptoms. CONCLUSIONS: a high frequency of sensitization to Bombyx mori was observed in a selected population of patients with respiratory allergic diseases in the city of Curitiba, state of Paraná, Brazil. The extract prepared from the wings of this moth species is effective in demonstrating this sensitivity. .

OBJETIVO: preparar extrato antigênico da mariposa do bicho-da-seda (Bombyx mori) e realizar testes cutâneos com esse extrato em pacientes com doenças respiratórias alérgicas, avaliar IgE sérica específica para Bombyx mori usando o sistema ImmunoCAP(r) e comparar a frequência de positividade entre os dois métodos e com dados clínicos. MÉTODOS: Estudo transversal com 99 crianças e adolescentes com diagnóstico de asma e/ou rinite alérgica, que apresentaram reação cutânea a pelo menos um dos seis aeroalérgenos testados. Os dados clínicos foram avaliados; testes cutâneos com extrato de Bombyx mori e análise de IgE total e específica por ImmunoCAP(r) foram realizados. RESULTADOS: a frequência de IgE específica para Bombyx mori foi de 52,5% e 60%, respectivamente, pelo teste cutâneo e ImmunoCAP(r). Foi observada uma associação entre o teste cutâneo positivo para Bombyx mori e a presença de rinite alérgica, dermatite atópica e urticária, mas o mesmo não ocorreu para a asma ou conjuntivite alérgica. Não houve relação com a gravidade dos sintomas de asma ou rinite. CONCLUSÕES: alta frequência de sensibilização à Bombyx mori foi encontrada em uma população selecionada de pacientes com doenças alérgicas respiratórias na cidade de Curitiba, estado do Paraná, Brasil. O extrato preparado a partir das asas dessa espécie de mariposa é eficaz em demonstrar essa sensibilidade. .
Descritores: Alérgenos/imunologia
Asma/imunologia
Bombyx/imunologia
Rinite Alérgica Perene/imunologia
-Asma/epidemiologia
Brasil
Estudos Transversais
Imunoglobulina E/sangue
Mariposas/imunologia
Rinite Alérgica
Rinite Alérgica Perene/epidemiologia
Testes Cutâneos/métodos
Limites: Adolescente
Animais
Criança
Feminino
Humanos
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-658761
Autor: Karina, Mancini; Dolder, Heidi.
Título: Dichotomic spermiogenesis in Euptoieta hegesia(Lepidoptera: Nymphalidae)
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;21(1):13-23, Jan.-Mar. 2004. ilus.
Idioma: en.
Resumo: Butterflies and moths produce enucleate (apyrene) and nucleate (eupyrene) spermatozoa. However, most studies oflepidopteran spermatogenesis and spermiogenesis have used only larvae and pupae. In this work, we used light andtransmission electron microscopy to examine spermiogenesis in males of the butterfly Euptoieta hegesia. Only adultmales were used, because this species has a long adult lifespan during which all cell stages can be observed. Male E.hegesia had a single fused testis with cysts that exclusively contained either apyrene or eupyrene cells. The mainevents of apyrene spermiogenesis included the formation, transformation and elimination of micronuclei, dense capformation, the development of mitochondrial derivatives and tail elongation. Eupyrene spermiogenesis involvedacrosome formation, nuclear condensation and elongation, extracellular appendage development, formation ofmitochondrial derivatives and tail elongation. The pattern and events of apyrene and eupyrene spermiogenesis in E.hegesia corroborate and complement data in the literature, particularly with regard to the intermediate developmentalstages of some structures, such as the acrosome, axoneme and extracellular appendages.
Descritores: Fenômenos Fisiológicos Celulares
Mariposas/anatomia & histologia
Mariposas/ultraestrutura
Espermatogênese
Testículo/crescimento & desenvolvimento
-Borboletas/anatomia & histologia
Borboletas/ultraestrutura
Microscopia Eletrônica
Limites: Animais
Masculino
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL



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