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Silva, Maria de Fátima Alves da
Id: lil-66618
Autor: Silva, Maria de Fátima Alves da; Landshoff, Flávia Martellini; Buononato, Marcus; Zelnik, Raymond.
Título: A ocorrência de taurina na anêmona-do-mar phyllacts flosculifera lesueur 1817 / The occurrence of taurine in sea anemones phyllacts flosculifera lesueur 1817
Fonte: Mem. Inst. Butantan;50(3):77-81, 1988.
Idioma: pt.
Resumo: A anêmona-do-mar Phyllactis flosculifera lesueur 1817 foi submetida a um tratamento extrativo com acetona e metanol e os extratos cromatografados em colunas de Sephadex LH-20, resultando no isolamento de um composto organo-sulfurado que foi identificado por espectrometria no infravermelho como sendo a taurina. O papel biológico deste aminoácido em invertebrados marinhos é discutido
Descritores: Taurina/toxicidade
Anêmonas-do-Mar
Responsável: BR91.2 - Centro de Documentação


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Texto completo
Id: biblio-1087698
Autor: Liu, Shu; Ahmed, Sibtain; Zhang, Chunguang; Liu, Tongxiao; Shao, Changlun; Fang, Yaowei.
Título: Diversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica
Fonte: Electron. j. biotechnol;44:41-46, Mar. 2020. tab, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Jiangsu Province Marine Science and Technology Innovation Project; . Priority Academic Program Development of Jiangsu Higher Education; . Institutions, Six Talent Peaks Project in Jiangsu Province.
Resumo: Background: The main objective of this study was to isolate fungi associated with Anthopleura xanthogrammica and measure their antimicrobial and enzymatic activities. A total of 93 fungal strains associated with A. xanthogrammica were isolated in this study, of which 32 isolates were identified using both morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The antibacterial activities of 32 fungal isolates were tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Edwardsiella tarda, Vibrio harveyi, Fusarium oxysporum, and Pyricularia oryzae by agar diffusion assay. Extracellular hydrolytic enzyme activities of the fungal isolates were determined by agar diffusion assays. Enzyme activities were detected from clear halo size. Results: The isolated fungi belonged to 18 genera within 7 taxonomic orders of 1 phylum. The genera Aspergillaceae were the most diverse and common. The antimicrobial activities of 32 isolates were evaluated, and 19 (59.4%) of fungi isolate displayed unique antimicrobial activities. All fungal strains displayed at least one enzyme activity. The most common enzyme activities in the fungi isolates were amylase and protease, while the least common were pectinase and xylanase. Conclusions: This is first report on the sea anemone-derived fungi with antimicrobial and enzyme activities. Results indicated that sea anemone is a hot spot of fungal diversity and a rich resource of bioactive natural products.
Descritores: Aspergillus/isolamento & purificação
Anêmonas-do-Mar/microbiologia
Antibacterianos/isolamento & purificação
-Peptídeo Hidrolases/metabolismo
Filogenia
Poligalacturonase/metabolismo
Aspergillus/enzimologia
Aspergillus/genética
Bactérias/efeitos dos fármacos
DNA Espaçador Ribossômico
Biodiversidade
Fungos/isolamento & purificação
Fungos/genética
Amilases/metabolismo
Antibacterianos/farmacologia
Responsável: CL1.1 - Biblioteca Central


  3 / 15 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: biblio-984697
Autor: Ramírez-Carreto, Santos; Pérez-García, Erick I; Salazar-García, Sandra I; Bernáldez-Sarabia, Johanna; Licea-Navarro, Alexei; Rudiño-Piñera, Enrique; Pérez-Martínez, Leonor; Pedraza-Alva, Gustavo; Rodríguez-Almazán, Claudia.
Título: Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Fonte: J. venom. anim. toxins incl. trop. dis;25:e147418, 2019. graf, ilus.
Idioma: en.
Projeto: CONACyT; . PAPIIT; . CONACyT; . CAPES; . CNPq; . CAPES.
Resumo: Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein from Anthopleura dowii Verrill (1869). 17. Methods: Cytolytic activity of A. dowii Verrill (1869) venom was determined via hemolysis assay in the erythrocytes of four mammals (sheep, goat, human and rabbit). The cytotoxic activity was analyzed in the human adherent lung carcinoma epithelial cells (A549) by the cytosolic lactate dehydrogenase (LDH) assay, and trypan blue staining. The venom was fractionated via ammonium sulfate precipitation gradient, dialysis, and ion exchange chromatography. The presence of a pore-forming protein in purified fractions was evaluated through hemolytic and cytotoxic assays, and the activity fraction was analyzed using the percent of osmotic protections after polyethylene glycol (PEG) treatment and mass spectrometry. 18. Results: The amount of protein at which the venom produced 50% hemolysis (HU50) was determined in hemolysis assays using erythrocytes from sheep (HU50 = 10.7 ± 0.2 µg), goat (HU50 = 13.2 ± 0.3 µg), rabbit (HU50 = 34.7 ± 0.5 µg), and human (HU50 = 25.6 ± 0.6 µg). The venom presented a cytotoxic effect in A549 cells and the protein amount present in the venom responsible for producing 50% death (IC50) was determined using a trypan blue cytotoxicity assay (1.84 ± 0.40 µg/mL). The loss of membrane integrity in the A549 cells caused by the venom was detected by the release of LDH in proportion to the amount of protein. The venom was fractionated; and the fraction with hemolytic and cytotoxic activities was analyzed by mass spectrometry. A pore-forming protein was identified. The cytotoxicity in the A549 cells produced by the fraction containing the pore-forming protein was osmotically protected by PEG-3350 Da molecular mass, which corroborated that the loss of integrity in the plasma membrane was produced via pore formation. 19. Conclusion: A. dowii Verrill (1869) venom contains a pore-forming protein suitable for designing new drugs for cancer therapy.(AU)
Descritores: Anêmonas-do-Mar
Venenos de Cnidários/isolamento & purificação
Neoplasias Pulmonares/terapia
-Venenos/toxicidade
Espectrometria de Massas/métodos
Células A549
Limites: Humanos
Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


  4 / 15 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-618190
Autor: Thangaraj, S; Bragadeeswaran, S.
Título: Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):53-61, 2012. ilus.
Idioma: en.
Resumo: Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12 percent gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea.(AU)
Descritores: Anêmonas-do-Mar/química
Antivenenos
Venenos de Cnidários/toxicidade
Neurotoxinas/química
-Bioensaio/métodos
Proteínas Hemolisinas/isolamento & purificação
Proteínas Hemolisinas/efeitos dos fármacos
Analgésicos/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


  5 / 15 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-468188
Autor: Avila, Ana D; Calderón, Carlos F; Perez, Rita M; Pons, Carmen; Pereda, Celia M; Ortiz, Ana R.
Título: Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
Fonte: Biol. Res;40(2):173-183, 2007. ilus, graf.
Idioma: en.
Resumo: Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor.
Descritores: Anticorpos Monoclonais/química
Hemólise/efeitos dos fármacos
Imunotoxinas/química
Receptores ErbB/metabolismo
Anêmonas-do-Mar/química
Células Tumorais Cultivadas/efeitos dos fármacos
-Anticorpos Monoclonais/imunologia
Anticorpos Monoclonais/metabolismo
Eletroforese em Gel de Poliacrilamida
Imuno-Histoquímica
Imunotoxinas/farmacologia
Receptores ErbB/efeitos dos fármacos
Células Tumorais Cultivadas/citologia
Limites: Animais
Humanos
Camundongos
Responsável: BR1.1 - BIREME


  6 / 15 LILACS  
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Texto completo SciELO Costa Rica
Texto completo
Id: lil-365971
Autor: Gomes, Paula Braga; Zamponi, Mauricio Oscar; Solé-Cava, Antonio Mateo.
Título: Asexual reproduction and molecular systematics of the sea anemone Anthopleura krebsi (Actiniaria: Actiniidae)
Fonte: Rev. biol. trop;51(1):147-154, mar. 2003. tab.
Idioma: en.
Resumo: In this paper we use allozyme analyses to demonstrate that individuals in Anthopleura krebsi aggregates are monoclonal. Additionally, sympatric samples of the red and the green colour-morphs of A. krebsi from Pernambuco, Brazil were genetically compared and no significant differences were observed between them (gene identity = 0.992), indicating that they do not belong to different biological species. All individuals within aggregates of the green colour-morph were found to be identical over the five polymorphic loci analysed. Such results would be extremely unlikely (P < 10(-11)) if the individuals analysed had been generated through sexual reproduction, thus confirming the presence of asexual reproduction in this species.
Descritores: Isoenzimas
Reprodução Assexuada
Anêmonas-do-Mar
-Brasil
Marcadores Genéticos
Reprodução Assexuada
Anêmonas-do-Mar
Limites: Animais
Responsável: BR1.1 - BIREME


  7 / 15 LILACS  
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Texto completo
Id: lil-299665
Autor: Díaz Concepción, Alina; Brito Martínez, Alberto; Tejuca Martínez, Mayra; Alvarez Varcárcel, Carlos; Lanio, María E; Almagro Vázquez, Delfina; Estrada del Cueto, Marianela.
Título: Efecto agregante plaquetario de 2 citolisinas: sti y stii purificadas de la anémona marina Stichodactyla helianthus / Platelet aggregation effect of 2 cytolysins: sti and stii purified from the sea anemone Stichodactyla helianthus
Fonte: Rev. cuba. hematol. inmunol. hemoter;15(3):182-189, sept.-dic. 1999. graf.
Idioma: es.
Resumo: Las citolisinas Sticholysina I (St I) y Sticholysina II (St II) inducen la agregación plaquetaria en el plasma rico en plaquetas en el rango de concentraciones ensayadas (0,5 a 10 µg/mL). Para ambas citolisinas se obtienen porcentajes de agregación plaquetaria superiores al 90 porciento con menos del 50 porciento de lisis celular. La agregación plaquetaria se mantiene elevada aún cuando la lisis celular disminuye a menos del 20 porciento. El EDTA 2 mM/L y el verapamilo 100 mM/L inhiben significativamente la agregación inducida por StI, lo que evidencia que el calcio extracelular tiene una función importante en este proceso y probablemente esta citolisina tiene una función similar a la de un ionóforo de calcio. Con StII no se obtuvo inhibición significativa de la agregación en presencia de EDTA y verapamilo. La agregación inducida por ambas citolisinas no está influida por el aumento del AMPc intracelular y es independiente de la formación de tromboxano A2 en la plaqueta
Descritores: Cromatografia em Gel/métodos
Cromatografia por Troca Iônica/métodos
Citotoxinas
Agregação Plaquetária
Anêmonas-do-Mar
Responsável: CU1.1 - Biblioteca Médica Nacional


  8 / 15 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: lil-298666
Autor: Lagos, P; Duran, R; Cerveñansky, C; Freitas, J. C; Silveira, R.
Título: Identification of hemolytic and neuroactive fractions in the venom of the sea anemone Bunodosoma cangicum
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(7):895-902, July 2001. ilus.
Idioma: en.
Resumo: Sea anemones are a rich source of biologically active substances. In crayfish muscle fibers, Bunodosoma cangicum whole venom selectively blocks the I K(Ca) currents. In the present study, we report for the first time powerful hemolytic and neuroactive effects present in two different fractions obtained by gel-filtration chromatography from whole venom of B. cangicum. A cytolytic fraction (Bcg-2) with components of molecular mass ranging from 8 to 18 kDa elicited hemolysis of mouse erythrocytes with an EC50 = 14 æg/ml and a maximum dose of 22 æg/ml. The effects of the neuroactive fraction, Bcg-3 (2 to 5 kDa), were studied on isolated crab nerves. This fraction prolonged the compound action potentials by increasing their duration and rise time in a dose-dependent manner. This effect was evident after the washout of the preparation, suggesting the existence of a reversible substance that was initially masking the effects of an irreversible one. In order to elucidate the target of Bcg-3 action, the fraction was applied to a tetraethylammonium-pretreated preparation. An additional increase in action potential duration was observed, suggesting a blockade of a different population of K+ channels or of tetraethylammonium-insensitive channels. Also, tetrodotoxin could not block the action potentials in a Bcg-3-pretreated preparation, suggesting a possible interaction of Bcg-3 with Na+ channels. The present data suggest that B. cangicum venom contains at least two bioactive fractions whose activity on cell membranes seems to differ from the I K(Ca) blockade described previously
Descritores: Braquiúros/efeitos dos fármacos
Venenos de Cnidários/farmacologia
Hemólise/efeitos dos fármacos
Neurotoxinas/farmacologia
Anêmonas-do-Mar
-Análise de Variância
Cromatografia em Gel
Venenos de Cnidários/isolamento & purificação
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


  9 / 15 LILACS  
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Texto completo SciELO Brasil
Cavalheiro, E. A
Carvalho, K. M
Texto completo
Id: lil-285856
Autor: Santana, A. N. C; Trindade-Filho, E. M; Cunha, R. B; Sousa, M. V; Cavalheiro, E. A; Carvalho, K. M.
Título: Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(6):797-801, Jun. 2001. ilus.
Idioma: en.
Resumo: In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation, rearing, jumping, barrel-rolling, wet dog shakes and forelimb clonic movements. Within 40-50 min, the status epilepticus was established and lasted 8-12 h. These results are similar to those observed in the acute phase of the pilocarpine model of temporal lobe epilepsy and suggest that granulitoxin may be a useful tool not only to study the sodium channels, but also to develop a new experimental model of status epilepticus.
Descritores: Comportamento Animal/efeitos dos fármacos
Eletroencefalografia/métodos
Neurotoxinas/toxicidade
Peptídeos/toxicidade
Anêmonas-do-Mar
Convulsões/induzido quimicamente
-Venenos de Cnidários/toxicidade
Hipocampo/efeitos dos fármacos
Microinjeções
Ratos Wistar
Convulsões/fisiopatologia
Fatores de Tempo
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 15 LILACS  
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Texto completo SciELO Brasil
Vale, O. C
Carvalho, K. M
Texto completo
Id: lil-223996
Autor: Santana, A. N. C; Leite, A. B; França, M. S. F; França, L; Vale, O. C; Cunha, R. B; Ricart, C. A. O; Sousa, M. V; Carvalho, K. M.
Título: Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;31(10):1335-8, Oct. 1998. ilus.
Idioma: en.
Conferência: Apresentado em: Annual Meeting of the Federaçäo de Sociedades de Biologia Experimental, 13, Caxambu, 26-29 Aug. 1998.
Resumo: A neurotoxic peptide, granulitoxin (GRX), was isolated from the sea anemone Bunodosoma granulifera. The N-terminal amino acid sequence of GRX is AKTGILDSDGPTVAGNSLSGT and its molecular mass is 4958 Da by electrospray mass spectrometry. This sequence presents a partial degree of homology with other toxins from sea anemones such as Bunodosoma caissarum, Anthopleura fuscoviridis and Anemonia sulcata. However, important differences were found: the first six amino acids of the sequence are different, Arg-14 was replaced by Ala and no cysteine residues were present in the partial sequence, while two cysteine residues were present in the first 21 amino acids of other toxins described above. Purified GRX injected ip (800 µg/kg) into mice produced severe neurotoxic effects such as circular movements, aggressive behavior, dyspnea, tonic-clonic convulsion and death. The 2-h LD50 of GRX was 400 ñ 83 µg/kg
Descritores: Neurotoxinas/química
Peptídeos/toxicidade
Anêmonas-do-Mar
-Sequência de Aminoácidos
Venenos de Cnidários
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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