Base de dados : LILACS
Pesquisa : B01.300.107 [Categoria DeCS]
Referências encontradas : 132 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 14 ir para página                         

  1 / 132 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974323
Autor: Wang, Xiuwen; Zhang, Xiaohua; Yao, Qingshou; Hua, Dongliang; Qin, Jiayang.
Título: Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol
Fonte: Braz. j. microbiol;49(supl.1):160-165, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Shandong Province Science and Technology Project; . Jinan Youth Science and Technology Star Project.
Resumo: Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.
Descritores: Ascomicetos/metabolismo
Proteínas Fúngicas/metabolismo
Diterpenos/metabolismo
-Ascomicetos/genética
Ascomicetos/química
Proteínas Fúngicas/química
Carbono/metabolismo
Eletroforese em Gel Bidimensional
Regulação Fúngica da Expressão Gênica
Proteômica
Glucose/metabolismo
Responsável: BR1.1 - BIREME


  2 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Mariano, Mário
Silva, Maria Regina Regis
Texto completo
Id: biblio-983752
Autor: Ogawa, Marilia Marufuji; Mariano, Mario; Silva, Maria Regina Regis; Enokihara, Milvia Maria Simões e Silva; Michalany, Nilceo Schwery; Nishikaku, Angela Satie; Silvestre, Agenor Messias; Tomimori, Jane.
Título: Study of tissue inflammatory response in different mice strains infected by dematiaceous fungi Fonsecaea pedrosoi
Fonte: An. bras. dermatol;94(1):29-36, Jan.-Feb. 2019. graf.
Idioma: en.
Resumo: Abstract: Background: Diseases caused by melanized fungi include mycetoma, chromoblastomycosis and phaeohyphomycosis. This broad clinical spectrum depends on the dynamic interactions between etiologic agent and host. The immune status of the host influences on the development of the disease, as, an exemple. phaeohyphomicosis is more frequently observed in immunocompromised patients. Objectives: Examine the histological inflammatory response induced by Fonsecaea pedrosoi in several different strains of mice (BALB/c, C57BL/6, Nude and SCID, and reconstituted Nude). Methods: Fonsecaea pedrosoi was cultivated on agar gel and a fragment of this gel was implanted subcutaneously in the abdominal region of female adult mice. After infection has been obtained, tissue fragment was studied histopathologically. Results: There were significant changes across the strains, with the nodular lesion more persistent in Nude and SCID mice, whereas in immunocompetent mice the lesion progressed to ulceration and healing. The histopathological analysis showed a significant acute inflammatory reaction which consisted mainly of neutrophils in the initial phase that was subsequently followed by a tuberculoid type granuloma in immunocompetent mice. Study limitations: There is no a suitable animal model for chromoblastomycosis. Conclusions: The neutrophilic infiltration had an important role in the containment of infection to prevent fungal spreading, including in immunodeficient mice. The fungal elimination was dependent on T lymphocytes. The re-exposure of C57BL/6 mice to Fonsecaea pedrosoi caused a delay in resolving the infection, and appearance of muriform cells, which may indicate that re-exposure to fungi, might lead to chronicity of infection.
Descritores: Ascomicetos
Dermatomicoses/imunologia
Imunocompetência
Inflamação/imunologia
Inflamação/microbiologia
-Especificidade da Espécie
Fatores de Tempo
Contagem de Células Sanguíneas
Doença Crônica
Cromoblastomicose/imunologia
Cromoblastomicose/patologia
Camundongos SCID
Dermatomicoses/patologia
Modelos Animais de Doenças
Inflamação/patologia
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Nus
Neutrófilos
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


  3 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828195
Autor: Jadon, Kuldeep Singh; Shah, Rakesh; Gour, Hari Narayan; Sharma, Pankaj.
Título: Management of blight of bell pepper (Capsicum annuum var. grossum) caused by Drechslera bicolor
Fonte: Braz. j. microbiol;47(4):1020-1029, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: Abstract Sweet or bell pepper is a member of the Solanaceae family and is regarded as one of the most popular and nutritious vegetable. Blight, in the form of leaf and fruit blight, has been observed to infect bell pepper crops cultivated at the horticulture farm in Rajasthan College of Agriculture, Udaipur, India. Based on disease severity, we attempted to curb this newly emerged problem using different fungicides, plant extracts, bio-control agents, and commercial botanicals against the fungus in laboratory and pot experiments. Bio-control agent Trichoderma viride and plant growth promoting Rhizobacteria (PGPR) isolate Neist-2 were found to be quite effective against bell pepper blight. All evaluated fungicides, botanicals, commercial botanicals, and bio-control agents in vitro were further studied as seed dressers and two foliar sprays at ten days interval in pot experiments. The combinations of Vitavax, PGPR isolate Neist-2, and Mehandi extract were found to be very effective against bell pepper blight followed by Vitavax, T. viride, and Mehandi extract used individually. All treatments in the pot experiments were found to significantly reduce seedling mortality and enhance plant biomass of bell pepper. Thus, these experimental findings suggest that a better integrated management of bell pepper blight could be achieved by conducting field trials in major bell pepper- and chilli-cultivated areas of the state. Besides fungicides, different botanicals and commercial botanicals also seem to be promising treatment options. Therefore, the outcome of the present study provides an alternate option of fungicide use in minimizing loss caused by Drechslera bicolor.
Descritores: Doenças das Plantas/microbiologia
Ascomicetos
Capsicum/microbiologia
-Ascomicetos/efeitos dos fármacos
Capsicum/química
Extratos Vegetais/farmacologia
Extratos Vegetais/química
Testes de Sensibilidade Microbiana
Plântulas/efeitos dos fármacos
Antifúngicos/farmacologia
Antifúngicos/química
Responsável: BR1.1 - BIREME


  4 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-788964
Autor: Corrêa, Juliana Moço; Christi, Divair; Torre, Carla Lieko Della; Henn, Caroline; Conceição-Silva, José Luis da; Kadowaki, Marina Kimiko; Simão, Rita de Cássia Garcia.
Título: High levels of ß-xylosidase in Thermomyces lanuginosus: potential use for saccharification
Fonte: Braz. j. microbiol;47(3):680-690, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: CAPES; . Araucária Foundation; . National Council for Scientific and Technological Development.
Resumo: ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of β-xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the β-xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production.
Descritores: Ascomicetos/enzimologia
Xilosidases/metabolismo
Fermentação
-Polissacarídeos/metabolismo
Polissacarídeos/química
Especificidade por Substrato
Temperatura Ambiente
Xilose/metabolismo
Biomassa
Zea mays/química
Ativação Enzimática
Concentração de Íons de Hidrogênio
Hidrólise
Responsável: BR1.1 - BIREME


  5 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-839351
Autor: An, Chao; Ma, Sai-jian; Chang, Fan; Xue, Wen-jiao.
Título: Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose
Fonte: Braz. j. microbiol;48(1):180-185, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technologic Research Program of Shaanxi Academy of Sciences, China; . WesternChinese Academy of Sciences; . Natural Science Basic Research Plan in Shaanxi Province of China; . Shaanxi Science & Technology Co-ordination & Innovation Project; . Science and Technology Program of Xi’an.
Resumo: Abstract Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using potato starch hydrolysate as a low-cost substrate for liquid-state fermentation by Aureobasidium pullulans. Based on fermentation kinetics evaluation of pullulan production by A. pullulans 201253, the pullulan production rate of A. pullulans with mixtures of potato starch hydrolysate and sucrose (potato starch hydrolysate:sucrose = 80:20) was 0.212 h−1, which was significantly higher than those of potato starch hydrolysate alone (0.146 h−1) and mixtures of potato starch hydrolysate, glucose, and fructose (potato starch hydrolysate:glucose:fructose = 80:10:10, 0.166 h−1) with 100 g L−1 total carbon source. The results suggest that mixtures of potato starch hydrolysate and sucrose could promote pullulan synthesis and possibly that a small amount of sucrose stimulated the enzyme responsible for pullulan synthesis and promoted effective potato starch hydrolysate conversion effectively. Thus, mixed sugars in potato starch hydrolysate and sucrose fermentation might be a promising alternative for the economical production of pullulan.
Descritores: Ascomicetos/metabolismo
Amido/metabolismo
Sacarose/metabolismo
Solanum tuberosum/química
Fermentação
Glucanos/biossíntese
-Amido/química
Carbono/metabolismo
Cinética
Biomassa
Reatores Biológicos
Técnicas de Cultura Celular por Lotes
Responsável: BR1.1 - BIREME


  6 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-950049
Autor: Astolfo, Ma. Florencia; Cañazares, Patricia; Majek, Elena; Burgesser, Virginia; Caruso, Martín; Basco, José; Alvarado, César; Carnovale, Susana.
Título: Sinusitis aguda invasiva por Exserohilum rostratum en una paciente pediátrica con recaída medular de leucemia linfoblástica aguda / Invasive acute sinusitis by Exserohilum rostratum in a patient with medullary relapse of acute lymphoblastic leukemia
Fonte: Arch. argent. pediatr;116(4):594-598, ago. 2018. ilus, tab.
Idioma: es.
Resumo: La infección fúngica invasora ha aumentado en frecuencia a lo largo de la última década, y la sinusitis fúngica es cada vez más habitual. Los hongos del género Exserohilum (familia Pleosporaceae, orden Pleosporales) son filamentosos y dematiáceos, de localización ubicua. Se trata de patógenos emergentes, que producen, en la mayoría de los casos, infecciones sistémicas que afectan, principalmente, a los senos paranasales y los pulmones. Son más frecuentes en pacientes inmunosuprimidos, aunque pueden presentarse en pacientes inmunocompetentes. El tratamiento de estas infecciones comprende el tratamiento antifúngico, resección quirúrgica y restitución de la inmunidad. Se presenta el caso de una paciente con recaída medular de leucemia linfoblástica aguda con sinusitis fúngica invasiva por Exserohilum rostratum.

Invasive fungal infection has increased in frequency over the last decade, with fungal sinusitis becoming more frequent. The fungi of the genus Exserohilum (family Pleosporaceae, order Pleosporales) are filamentous and dematiaceous of ubiquitous location. It is an emerging pathogen, which in most cases produces a systemic infection that mainly affects the paranasal sinuses and lungs. It is more common in immunosuppressed patients, although it may occur in immunocompetent patients. The treatment is based on three pillars: antifungal treatment, surgical debridement and restitution of immunity. We present the case of a patient with medullary relapse of acute lymphoblastic leukemia with invasive fungal sinusitis by Exserohilum rostratum.
Descritores: Ascomicetos/isolamento & purificação
Sinusite/diagnóstico
Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia
Infecções Fúngicas Invasivas/diagnóstico
-Recidiva
Sinusite/microbiologia
Sinusite/terapia
Doença Aguda
Desbridamento/métodos
Infecções Fúngicas Invasivas/microbiologia
Infecções Fúngicas Invasivas/terapia
Antifúngicos/uso terapêutico
Limites: Seres Humanos
Feminino
Pré-Escolar
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


  7 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-887414
Autor: Campoverde Espinoza, Christian J; Carballo, Carolina M; Orlando, M. Nancy; Hevia, Alejandra I; Gómez Raccio, Andrea C; Di Giovanni, Daniela; Bezrodnik, Liliana; Vázquez, Miryam S; Cazes, Claudia I; López, Eduardo L.
Título: Infección pulmonar por Arthrographis kalrae en un paciente con enfermedad granulomatosa crónica / Pulmonary infection by Arthrographis kalrae in patient with chronic granulomatous disease
Fonte: Arch. argent. pediatr;115(6):458-461, dic. 2017. ilus.
Idioma: es.
Resumo: Antecedentes: Arthrographis kalrae es un hongo hialino de crecimiento lento que, en su desarrollo, forma artroconidios. Es un patógeno oportunista que causa infecciones en personas inmunocomprometidas e inmunocompetentes, y ha sido aislado muy raramente en muestras clínicas de seres humanos. Caso clínico: Se describe el caso de un paciente con inmunodeficiencia primaria y afectación pulmonar con evolución tórpida. Presentó compromiso de ambos pulmones a pesar del tratamiento antibiótico y antifúngico instaurado. Durante su seguimiento, se realizaron múltiples biopsias pulmonares y se aisló A. kalrae en el cultivo de tejido pulmonar. Recibió tratamiento con posaconazol, con buena respuesta y remisión de las lesiones. Conclusión: Este es el primer caso reportado de infección pulmonar por A. kalrae en un paciente pediátrico con enfermedad granulomatosa crónica en Argentina.

Background: Arthrographis kalrae is a hyaline fungus that grows forming arthroconidia. It is an opportunistic pathogen that causes infections in immunocompromised as in immunocompetent people and has been rarely isolated from human clinical samples. Case report: We describe the case of a male child with primary immunodeficiency who initially presented unilateral pneumonia and progressed to bilateral involvement despite antibiotic, antifungal treatment. A. kalrae was diagnosed by pulmonary biopsy. He received posaconazole with resolution of disease. Conclusions: This is the first case of A. kalrae pulmonary infection in a pediatric patient with chronic granulomatous disease in Argentina.
Descritores: Ascomicetos
Doença Granulomatosa Crônica/complicações
Pneumopatias Fúngicas/microbiologia
Micoses/complicações
-Pneumopatias Fúngicas/tratamento farmacológico
Micoses/microbiologia
Micoses/tratamento farmacológico
Limites: Seres Humanos
Masculino
Pré-Escolar
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


  8 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-968957
Autor: Pestana, Jeferson Rodrigo; Morais, Tâmara Prado de; Nogueira, Ana Paula Oliveira; Juliatti, Fernando Cezar.
Título: Reaction of brazilian cotton genotypes to white mold depends on pathogen aggressiveness and incubation conditions / Reação de genótipos brasileiros de algodoeiro ao mofo branco depende da agressividade do patógeno e das condições de incubação
Fonte: Biosci. j. (Online);34(6):1555-1574, nov./dec. 2018. tab, graf, ilus.
Idioma: en.
Resumo: The expansion of cotton crop into irrigated and high lands of Brazilian Cerrado, despite the possibility of increasing fiber yield, led to the occurrence of diseases previously considered secondary, such as white mold [Sclerotinia sclerotiorum (Lib.) de Bary]. Host genetic resistance is of extreme importance in integrated strategies to manage this disease. Resistance of Brazilian cotton genotypes, challenged with different strains of S. sclerotiorum, under two incubation conditions for disease progress was evaluated. In addition, possible correlation between oxalic acid and straw test methods to rank the genotypes was evaluated. Artificial inoculation was done when cotton plants reached the V2phenological stage with fungi isolated from naturally infected soybean (ScS) or cotton (ScC) commercial crops. Control plants were inoculated with culture medium. After inoculation, plants were kept for one week either in a growth chamber or in greenhouse and evaluated for disease symptoms and severity. The oxalic acid test consisted of stem submersion of rootless cotton plants in a 2-cm layer of 20 or 40 mM solutions for 20, 44 or 68 h. A wilting scale was used to distinguish genotype's sensibility to the acid. The data were submitted to individual, joint, and multivariate analysis, grouping cotton genotypes by the Scott-Knott's test (p < 0.05), the hierarchical UPGMA and the non-hierarchical Tocher methods. Difference in aggressiveness between strains was identified, in which ScC led to greater disease severity. This result suggests a possible physiological specialization ofS. sclerotiorum to different hosts. It was observed that the growth chamber environment provided more adequate conditions for S. sclerotiorum infection, thus allowing better selection of resistant cotton genotypes. UPGMA and Tocher grouping methods further confirmed that the evaluated genotypes differ from each other in resistance to white mold. No correlation between oxalic acid and straw test methods was observed.

A expansão da cultura do algodoeiro para terras altas e irrigadas do Cerrado brasileiro, apesar da possibilidade de aumentar a produção de fibras, levou à ocorrência de doenças antes consideradas secundárias, como o mofo branco [Sclerotinia sclerotiorum (Lib.) de Bary]. A resistência genética do hospedeiro é de extrema importância nas estratégias de manejo integrado dessa doença. Avaliou-se a resistência de genótipos brasileiros de algodão, desafiados com diferentes isolados de S. sclerotiorum, sob duas condições de incubação para o progresso da doença. Além disso, foi avaliada a possível correlação entre os métodos do ácido oxálico e do straw test para ranquear os genótipos. A inoculação artificial foi realizada quando as plantas de algodoeiro atingiram o estágio fenológico V2, com fungos isolados de culturas comerciais de soja (ScS) ou de algodão (ScC) naturalmente infectadas. O controle consistiu de plantas inoculadas somente com meio de cultura. Após a inoculação, as plantas foram mantidas em câmara de crescimento ou em casa de vegetação durante uma semana e avaliadas quanto aos sintomas e severidade da doença. O teste do ácido oxálico consistiu na submersão da haste das plantas de algodão, após remoção das raízes, em uma solução de 20 ou 40 mM por 20, 44 ou 68 h. Uma escala visual de murcha foi usada para distinguir a sensibilidade dos genótipos ao ácido. Os dados foram submetidos à análise individual, conjunta e multivariada, agrupando os genótipos de algodoeiro pelo teste de Scott-Knott (p < 0,05) e pelos métodos UPGMA e de Tocher. Diferença na agressividade entre os isolados foi identificada, na qual ScC resultou em maior severidade da doença. Isto sugere possível especialização fisiológica de S. sclerotiorum para diferentes hospedeiros. Observou-se que o ambiente da câmara de crescimento proporcionou condições mais adequadas para infecção por S. sclerotiorum comparativamente à casa de vegetação, permitindo melhor seleção de genótipos resistentes. Os métodos de agrupamento UPGMA e Tocher confirmaram que os genótipos avaliados diferem entre si na resistência ao mofo branco. Não foi observada correlação entre o ácido oxálico e o straw test.
Descritores: Ascomicetos
Variação Genética
Gossypium
-Ácido Oxálico
Pradaria
Noxas
Responsável: BR396.1 - Biblioteca Central


  9 / 132 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-889228
Autor: Ding, Chang-hong; Wang, Qian-Bo; Guo, Shenglei; Wang, Zhen-yue.
Título: The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi
Fonte: Braz. j. microbiol;49(2):362-369, Apr.-June 2018. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Heilongjiang Province.
Resumo: Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL-1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.
Descritores: Ascomicetos/metabolismo
Rumex/metabolismo
Rumex/microbiologia
Endófitos/metabolismo
Compostos Fitoquímicos/metabolismo
-Ascomicetos/isolamento & purificação
Ascomicetos/crescimento & desenvolvimento
Fatores de Tempo
Técnicas de Cocultura
Rumex/crescimento & desenvolvimento
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Plântulas/microbiologia
Endófitos/isolamento & purificação
Endófitos/crescimento & desenvolvimento
Limites: Seres Humanos
Adolescente
Responsável: BR1.1 - BIREME


  10 / 132 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Texto completo
Id: biblio-1025923
Autor: Zanella, Cláudia de Souza; Gavassoni, Walber Luiz; Bacchi, Lilian Maria Arruda; Formagio, Anelise Samara Nazari.
Título: Atividade de óleos e extratos vegetais sobre germinação carpogênica e crescimento micelial de Sclerotinia sclerotiorum / Activity of plant extracts on the carpogenic germination and mycelial growth of Sclerotinia sclerotiorum
Fonte: Arq. Inst. Biol;82:1-8, 2015. tab, graf.
Idioma: pt.
Resumo: A germinação carpogênica e o crescimento micelial de Sclerotinia sclerotiorum foram avaliados sob extratos metanólicos de Annona cacans, A. coriacea, A. crassiflora, A. dioica, A. sylvatica, Geophila repens, Guettarda viburnoides, Palicourea crocea, Schinus terebinthifolius e Trichilia silvatica, e sob as frações hexânica, hidrometanólica, clorofórmica e acetato de etila de A. cacans e óleo essencial de S. terebinthifolius. A concentração utilizada foi de 1.000 ppm para os extratos e de 100 ppm para as frações. Os extratos vegetais e as frações foram incorporados em meio ágar-água, que foi vertido em caixas gerbox com 20 escleródios. O crescimento micelial foi avaliado em óleo essencial de S. terebinthifolius, nas concentrações de 0, 100 e 1.000 ppm, incorporado ao meio BDA (Batata-Dextrose-Ágar). A germinação carpogênica apresentou-se menor sob os extratos de G. repens, P. crocea e S. terebinthifolius e sob as frações acetato de etila e clorofórmica de A. cacans. O número de apotécios formados por gerbox foi menor com o extrato de A. cacans. O crescimento micelial apresentou 10% de inibição na maior concentração do óleo essencial de S. terebinthifolius.(AU)

The efect of methanolic extracts of Annona cacans, A. coriacea, A. crassiflora, A. dioica, A. sylvatica, Geophila repens, Guettarda viburnoides, Palicourea crocea, Schinus terebinthifolius e Trichilia silvatica, and A. cacans hexane, ethyl etila, aqueous and chloroform fractions and the essential oil of S. terebinthifolius on mycelial growth and carpogenic germination of Sclerotinia sclerotiorum was evaluated. Te concentrations are 1,000 ppm for the extracts and 100 ppm for the fractions. To evaluate the germination, carpogenic, extracts and fractions were incorporated in agar-water that was poured into gerboxes where 20 sclerotia were distributed. To evaluate the mycelial growth, essential oil of S. terebinthifolius in concentrations of 0, 100 and 1,000 ppm was incorporated into the PDA and then poured into Petri dishes, to where pathogen mycelial discs were transferred. Extracts of G. repens, P. crocea and S. terebinthifolius and fractions ethyl acetate and chloroform of Annona cacans reduced the carpogenic germination of sclerotia of S. sclerotiorum and the extract of A. cacans reduced the number of apothecia formed. Mycelial growth showed 10% inhibition at the highest concentration of essential oil of S. terebinthifolius.(AU)
Descritores: Plantas
Plantas Medicinais
Ascomicetos
Óleos Voláteis
Fungos
-Controle de Pragas
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação



página 1 de 14 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde