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Id: biblio-1026812
Autor: Sulieman, Ashraf K; Putra, Meilana Dharma; Abasaeed, Ahmed E; Gaily, Mohamed H; Al-Zahrani, Saeed M; Zeinelabdeen, Mohamed A.
Título: Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;34:1-8, july. 2018. tab, graf.
Idioma: en.
Resumo: Background: Ethanol and fructose are two important industrial products that enjoy many uses. In this contribution, their production via selective fermentation of date extract using Saccharomyces cerevisiae was studied. Scaling up the process for possible commercialization was investigated in three fermentors with working volume ratio of 1:40:400. Results: Higher ethanol concentration was obtained in the larger fermentor due to conversion of fructose. Fructose yields in the 0.5-L, 7.5-L and 80-L fermentors were 99, 92 and 90%, respectively. Good fitting was obtained with the modified Monod kinetics; however, a better fit of cell mass was obtained with the modified Ghose­Tyagi model which accounts for ethanol inhibition. Conclusions: The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.
Descritores: Saccharomyces cerevisiae/metabolismo
Etanol/síntese química
Frutose/biossíntese
-Leveduras
Cinética
Reatores Biológicos
Fermentação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1051261
Autor: Massoud, Ramona; Hadiani, Mohammad Rasoul; Hamzehlou, Pegah; Khosravi-Darani, Kianoush.
Título: Bioremediation of heavy metals in food industry: application of Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;37:56-60, Jan. 2019. tab.
Idioma: en.
Projeto: Shahid Beheshti University of Medical Sciences.
Resumo: Heavy metals are natural elements in the Earth's crust that can enter human food through industrial or agricultural processing, in the form of fertilizers and pesticides. These elements are not biodegradable. Some heavy metals are known as pollutants and are toxic, and their bioaccumulation in plant and animal tissues can cause undesirable effects for humans; therefore, their amount in water and food should always be under control. The aim of this study is to investigate the conditions for the bioremediation of heavy metals in foods. Various physical, chemical, and biological methods have been used to reduce the heavy metal content in the environment. During the last decades, bioremediation methods using plants and microorganisms have created interest to researchers for their advantages such as being more specific and environmentally friendly. The main pollutant elements in foods and beverages are lead, cadmium, arsenic, and mercury, which have their own permissible limits. Among the microorganisms that are capable of bioremediation of heavy metals, Saccharomyces cerevisiae is an interesting choice for its special characteristics and being safe for humans, which make it quite common and useful in the food industry. Its mass production as the byproduct of the fermentation industry and the low cost of culture media are the other advantages. The ability of this yeast to remove an individual separated element has also been widely investigated. In countries with high heavy metal pollution in wheat, the use of S. cerevisiae is a native solution for overcoming the problem of solution. This article summarizes the main conditions for heavy metal absorption by S. cerevisiae.
Descritores: Saccharomyces cerevisiae/metabolismo
Biodegradação Ambiental
Indústria Alimentícia
Metais Pesados/metabolismo
-Arsênio
Leveduras
Cádmio
Remoção de Contaminantes
Absorção
Bioacumulação
Chumbo
Mercúrio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1254708
Autor: Putra, Meilana Dharma; Abasaeed, Ahmed E; Al-Zahrani, Saeed M.
Título: Prospective production of fructose and single cell protein from date palm waste
Fonte: Electron. j. biotechnol;48:46-52, nov. 2020. graf, tab.
Idioma: en.
Projeto: King Saud University.
Resumo: BACKGROUND: Fructose and single cell protein are important products for the food market. Abundant amounts of low-grade dates worldwide are annually wasted. In this study, highly concentrated fructose syrups and single cell protein were obtained through selective fermentation of date extracts by Saccharomyces cerevisiae. RESULTS: The effect of air flow (0.1, 0.5, 0.75, 1, 1.25 and 1.5 vvm) and pH (4.5, 4.8, 5, 5.3 and 5.6) was investigated. Higher air flow led to lower fructose yield. The optimum cell mass production of 10 g/L was achieved at air flow of 1.25 vvm with the fructose yield of 91%. Similar cell mass production was obtained in the range pH of 5.0­5.6, while less cell mass was obtained at pH less than 5. Controlling the pH at 4.5, 5.0 and 5.3 failed to improve the production of cell mass which were 5.6, 5.9 and 5.4 g/L respectively; however, better fructose yield was obtained. CONCLUSIONS: Extension of the modified Gompertz enabled excellent predictions of the cell mass, fructose production and fructose fraction. The proposed model was also successfully validated against data from literatures. Thus, the model will be useful for wide application of biological processes.
Descritores: Saccharomyces cerevisiae/fisiologia
Phoeniceae
Frutose/biossíntese
-Aerobiose
Conceitos Matemáticos
Fermentação
Resíduos de Alimentos
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1278453
Autor: Gok, Ozlem; Beyaz, Seda; Aslan, Abdullah.
Título: Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing
Fonte: Braz. arch. biol. technol;64:e21210002, 2021. tab, graf.
Idioma: en.
Resumo: Abstract In this study, the effects of Ellagic acid (EA) on protein expression in yeasts and cellular development were investigated. Four groups were formed. Groups: 1) Control group; yeast only cultivated group; 2) Ellagic Acid (EA) group: EA (10%) given group; 3) Hydrogen peroxide (H2O2) Group: The group given H2O2 (15 mM); 4) EA + H2O2 group: EA (10%) + H2O2 (15 mM) group. After sterilization, EA (10%) and H2O2 (15 mM) were added to the Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours (overnight). S. cerevisiae cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) level were analyzed by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and measured by the Bradford method. According to the obtained results, compared with the H2O2 group, cell development (1, 3, 5 and 24 hours), GSH level and total protein synthesis (24 hours) were increased with EA, while MDA level (24 hours) decreased. These results show that EA reduces oxidative damage, increases cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture.
Descritores: Saccharomyces cerevisiae
Eletroforese em Gel de Poliacrilamida
Ácido Elágico
Peróxido de Hidrogênio
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1223246
Autor: Sriputorn, Benjaporn; Laopaiboon, Pattana; Phukoetphim, Niphaphat; Polsokchuak, Nilubol; Butkun, Khanittha; Laopaiboon, Lakkana.
Título: Enhancement of ethanol production efficiency in repeated-batch fermentation from sweet sorghum stem juice: effect of initial sugar, nitrogen and aeration
Fonte: Electron. j. biotechnol;46:55-64, jul. 2020. tab, graf.
Idioma: en.
Projeto: Ph.D. Program; . Post-doctoral Program.
Resumo: BACKGROUND: Ethanol concentration (PE), ethanol productivity (QP) and sugar consumption (SC) are important values in industrial ethanol production. In this study, initial sugar and nitrogen (urea) concentrations in sweet sorghum stem juice (SSJ) were optimized for high PE (≥10%, v/v), QP, (≥2.5 g/L·h) and SC (≥90%) by Saccharomyces cerevisiae SSJKKU01. Then, repeated-batch fermentations under normal gravity (NG) and high gravity (HG) conditions were studied. RESULTS: The initial sugar at 208 g/L and urea at 2.75 g/L were the optimum values to meet the criteria. At the initial yeast cell concentration of ~1 × 108 cells/mL, the PE, QP and SC were 97.06 g/L, 3.24 g/L·h and 95.43%, respectively. Repeated-batch fermentations showed that the ethanol production efficiency of eight successive cycles with and without aeration were not significantly different when the initial sugar of cycles 2 to 8 was under NG conditions (~140 g/L). Positive effects of aeration were observed when the initial sugar from cycle 2 was under HG conditions (180­200 g/L). The PE and QP under no aeration were consecutively lower from cycle 1 to cycle 6. Additionally, aeration affected ergosterol formation in yeast cell membrane at high ethanol concentrations, whereas trehalose content under all conditions was not different. CONCLUSION: Initial sugar, sufficient nitrogen and appropriated aeration are necessary for promoting yeast growth and ethanol fermentation. The SSJ was successfully used as an ethanol production medium for a high level of ethanol production. Aeration was not essential for repeated-batch fermentation under NG conditions, but it was beneficial under HG conditions.
Descritores: Saccharomyces cerevisiae/metabolismo
Sorghum/química
Etanol/metabolismo
-Saccharomyces cerevisiae/crescimento & desenvolvimento
Ureia
Leveduras/crescimento & desenvolvimento
Aeração
Sorghum/microbiologia
Etanol/análise
Açúcares
Sucos
Fermentação
Gravitação
Nitrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1001065
Autor: Motta, Estela; Capristo, Rocío; Franco, Micaela.
Título: Prevalencia de anticuerpos anti-Saccharomyces cerevisiae en pacientes celíacos / Prevalence of anti-Saccharomyces cerevisiae antibodies in celiac patients / Prevalência de anticorpos anti-Saccharomyces cerevisiae em pacientes celíacos
Fonte: Acta bioquím. clín. latinoam;52(4):417-422, dic. 2018. graf.
Idioma: es.
Resumo: La enfermedad celíaca (EC) es una condición inflamatoria crónica del intestino delgado causada por intolerancia al gluten. El tratamiento consiste en la dieta libre de gluten (DLG). Los anticuerpos anti Saccharomyces cerevisiae (ASCA) están dirigidos contra la pared celular de la levadura, se asocian a enfermedades autoinmunes, y se propone la permeabilidad intestinal alterada como causa de activación de la inmunidad humoral. El objetivo del trabajo fue determinar la prevalencia de ASCA IgG e IgA en pacientes celíacos bajo tratamiento y evaluar la asociación de ASCA con el grado de adherencia a la DLG. Se analizaron 59 sueros de pacientes adultos celíacos con alta o baja adherencia a la DLG, y se determinó ASCA IgG e IgA. Se halló una prevalencia de ASCA IgG y/o IgA del 44%. Se encontró asociación entre ASCA-IgG y adherencia a DLG (OR 4,04 IC 95%: 1,32-12,38). La prevalencia de ASCA en la población celíaca estudiada es similar a la reportada en la bibliografía. La menor prevalencia de ASCA IgG en pacientes con una estricta DLG respecto de aquellos con baja adherencia, indicaría que su presencia depende del nivel de ingesta de gluten, sugiriéndolos como herramienta complementaria en el seguimiento del paciente celíaco.

Celiac disease (CD) is a chronic inflammatory condition of the small intestine caused by gluten intolerance. The treatment consists of gluten free diet (GFD). Anti Saccharomyces cerevisiae antibodies (ASCA) are directed against the cell wall of yeast, associated with autoimmune diseases, and an altered intestinal permeability is proposed as a cause of activation of humoral immunity. The objective of this work was to determine the prevalence of IgG and IgA ASCA in celiac patients under treatment and to evaluate the association of ASCA with the degree of adherence to GFD. Fifty-nine serum samples from adult celiac patients with high or low adherence to GFD were analyzed, determining IgG and IgA ASCA. A 44% prevalence of IgG and/or IgA ASCA was found. An association was discovered between IgG ASCA and GFD adherence (OR 4.04, 95% CI: 1.32-12.38). The prevalence of ASCA in the studied celiac population is similar to that reported in the literature. The lower prevalence of IgG ASCA in patients with a strict GFD compared to those with low adherence would indicate that their presence depends on the level of gluten intake, suggesting them as a complementary tool in the follow-up of the celiac patient.

A doença celíaca (DC) é uma condição inflamatória crônica do intestino delgado causada pela intolerância ao glúten. O tratamento consiste na dieta sem glúten (DSG). Os anticorpos anti Saccharomyces cerevisiae (ASCA) são dirigidos contra a parede celular da levedura, associados a doenças autoimunes, e à permeabilidade intestinal alterada como causa da ativação da imunidade humoral. O objetivo foi determinar a prevalência de ASCA IgG e IgA em pacientes celíacos em tratamento; avaliar a associação de ASCA com o grau de adesão ao DSG. Foram analisados 59 soros de pacientes celíacos adultos com alta ou baixa adesão ao DSG, determinando ASCA IgG e IgA. Foi encontrada uma prevalência de SCA IgG/ou IgA de 44%. Foi encontrada uma associação entre ASCA-IgG e a adesão ao DSG (OR 4,04 IC 95% 1,32-12,38). A prevalência de ASCA na população celíaca estudada é semelhante à relatada na literatura. A menor prevalência de ASCA IgG em pacientes com rigorosa DSG, em comparação àqueles com baixa adesão, indicaria que sua presença depende do nível de ingestão de glúten, sugerindo-os como uma ferramenta complementar no seguimento do paciente celíaco.
Descritores: Saccharomyces cerevisiae/imunologia
Doença Celíaca/dietoterapia
Doença Celíaca/microbiologia
Dieta Livre de Glúten
Cooperação e Adesão ao Tratamento
Anticorpos Antifúngicos/sangue
-Imunoglobulina A/imunologia
Imunoglobulina G/imunologia
Prevalência
Estudos Transversais
Estudos de Coortes
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Texto completo SciELO Chile
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Id: biblio-950732
Autor: Kabir, Md Golam; Rahman, Md Monsor; Ahmed, Nazim Uddin; Fakruddin, Md; Islam, Saiful; Mazumdar, Reaz Mohammad.
Título: Antioxidant, antimicrobial, toxicity and analgesic properties of ethanol extract of Solena amplexicaulis root
Fonte: Biol. Res;47:1-12, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: This study was subjected to investigate different pharmacological properties of ethanol extract ofSolena amplexicaulis root. RESULTS: The extract contains flavonoid, alkaloid, saponin and steroid compounds. The extract exhibited excellent antioxidant activity in DPPH radical scavenging activity. The extract also showed potent activity in brine shrimp lethality bioassay. The LC50 value was found to 44.677 µg/ml. The extract showed better anti-bacterial activity against gram-negative bacteria. In antifungal assay, the maximum 79.31% of anti-mycotic activity was observed against Aspergillus ochraceus while minimum 44.2% against Rhizopus oryzae. MIC value ranged between 1500 - 3000 µg/ml. The extract was found moderately toxic with a 24-hr LD50 value of 81.47 mg/kg in Swiss albino mice. The degree of inhibition by the ethanolic extract of the root was found less than that of standard analgesic drug diclofenac sodium. The extract also showed moderate anti-inflammatory and antinociceptive activity and anti-diabetic property. Reducing power of the extract was comparable with standard ascorbic acid. Moderate in vitro thrombolytic activity, lipid peroxidation inhibition property, metal chelating ability and stress-protective activity was also observed. CONCLUSION: Ethanol extract of Solena amplexicaulis root can be valuable for treatment of different diseases.
Descritores: Extratos Vegetais/farmacologia
Sequestradores de Radicais Livres/farmacologia
Raízes de Plantas/química
Cucurbitaceae/química
Analgésicos/farmacologia
Anti-Infecciosos/farmacologia
-Artemia/efeitos dos fármacos
Aspergillus/efeitos dos fármacos
Saccharomyces cerevisiae/efeitos dos fármacos
Shigella/efeitos dos fármacos
Bacillus/efeitos dos fármacos
Extratos Vegetais/química
Peroxidação de Lipídeos/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Quelantes/farmacologia
Substâncias Redutoras/farmacologia
Fibrinolíticos/farmacologia
Hipoglicemiantes/farmacologia
Dose Letal Mediana
Anti-Inflamatórios/farmacologia
Antibacterianos/farmacologia
Antifúngicos/farmacologia
Antioxidantes/farmacologia
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: biblio-950803
Autor: Vásquez-Soto, Beatriz; Manríquez, Nicolás; Cruz-Amaya, Mirna; Zouhar, Jan; Raikhel, Natasha V; Norambuena, Lorena.
Título: Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae
Fonte: Biol. Res;48:1-11, 2015. ilus, graf, tab.
Idioma: en.
Projeto: FONDECYT.
Resumo: BACKGROUND: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY), triggering its secretion in Saccharomyces cerevisiae. RESULTS: A reverse chemical-genetics approach was used to identify key proteins for Sortin2 bioactivity. A genome-wide Sortin2 resistance screen revealed six yeast deletion mutants that do not secrete CPY when grown at Sortin2 condition where the parental strain does: met18, sla1, clc1, dfg10, dpl1 and yjl175w. Integrating mutant phenotype and gene ontology annotation of the corresponding genes and their interactome pointed towards a high representation of genes involved in the endocytic process. In wild type yeast endocytosis towards the vacuole was faster in presence of Sortin2, which further validates the data of the genome-wide screen. This effect of Sortin2 depends on structural features of the molecule, suggesting compound specificity. Sortin2 did not affect endocytic trafficking in Sortin2-resistant mutants, strongly suggesting that the Sortin2 effects on the secretory and endocytic pathways are linked. CONCLUSIONS: Overall, the results reveal that Sortin2 enhances the endocytic transport pathway in Saccharomyces cerevisiae. This cellular effect is most likely at the level where secretory and endocytic pathways are merged. Them Sortin2 specificity over the endomembrane system places it as a powerful biological modulator for cell biology.
Descritores: Proteínas de Plantas/fisiologia
Rodanina/análogos & derivados
Saccharomyces cerevisiae/metabolismo
Vacúolos/metabolismo
Alcanossulfonatos/farmacologia
Transporte Proteico/genética
Endocitose/fisiologia
-Fenótipo
Rodanina/farmacologia
Vacúolos/fisiologia
Transporte Biológico
Via Secretória
Tipo de Publ: Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1236671
Autor: Piestun, Valeria Sedrani.
Título: Clonagem e expressao do gene da proteina de 18 kDa de Mycobacterium leprae em Saccharomyces cerevisiae / ?.
Fonte: Sao Paulo; s.n; 1992. 74 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de Sao Paul. Instituto de Ciencias Biomedicas para obtenção do grau de Master.
Resumo: A hanseniase e uma doenca infecciosa cronica causada pelo bacilo Mycobacterium leprae que atinge de 10 e 15 milhoes de pessoas em todo o mundo principalmente em areas tropicais e subtropicais. O desenvolvimento de um diagnostico preciso e de uma imunoprofilaxia eficiente sao fatores de qrande importancia no controle epidemiologico da doenca. Dentre os antigenos do M. leprae, a proteina de 18kDa mostrou-se uma das mais antigenicas, sendo responsavel pela proliferacao de linfocitos auxiliares "in vitro". O gene que codifica esta proteina foi identificado atraves da construcao de uma biblioteca em fago lamdba gtii, o que possibilitou sua clonagem e sequenciamento. Com a finalidade de se obter grandes quantidades dessa proteina em sua forma nao glicosada,
Descritores: Engenharia Genética
Mycobacterium leprae
Saccharomyces cerevisiae
Vetores Genéticos
Responsável: BR191.1 - Biblioteca e Centro de Documentação Luiza Keffer
[{"text": "BR191.1", "_a": "WC335.402", "_b": "P616c"}]


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Id: biblio-1089077
Autor: Kessi-Pérez, Eduardo I; Molinet, Jennifer; Martínez, Claudio.
Título: Disentangling the genetic bases of Saccharomyces cerevisiae nitrogen consumption and adaptation to low nitrogen environments in wine fermentation
Fonte: Biol. Res;53:02, 2020. tab, graf.
Idioma: en.
Projeto: Universidad de Santiago de Chile; . CONICYT/FONDECYT.
Resumo: The budding yeast Saccharomyces cerevisiae has been considered for more than 20 years as a premier model organ- ism for biological sciences, also being the main microorganism used in wide industrial applications, like alcoholic fermentation in the winemaking process. Grape juice is a challenging environment for S. cerevisiae , with nitrogen deficiencies impairing fermentation rate and yeast biomass production, causing stuck or sluggish fermentations, thus generating sizeable economic losses for wine industry. In the present review, we summarize some recent efforts in the search of causative genes that account for yeast adaptation to low nitrogen environments, specially focused in wine fermentation conditions. We start presenting a brief perspective of yeast nitrogen utilization under wine fermentative conditions, highlighting yeast preference for some nitrogen sources above others. Then, we give an outlook of S. cerevisiae genetic diversity studies, paying special attention to efforts in genome sequencing for population structure determination and presenting QTL mapping as a powerful tool for phenotype-genotype correlations. Finally, we do a recapitulation of S. cerevisiae natural diversity related to low nitrogen adaptation, specially showing how different studies have left in evidence the central role of the TORC1 signalling pathway in nitrogen utilization and positioned wild S. cerevisiae strains as a reservoir of beneficial alleles with potential industrial applications (e.g. improvement of industrial yeasts for wine production). More studies focused in disentangling the genetic bases of S. cerevisiae adaptation in wine fermentation will be key to determine the domestication effects over low nitrogen adaptation, as well as to definitely proof that wild S. cerevisiae strains have potential genetic determinants for better adaptation to low nitrogen conditions.
Descritores: Saccharomyces cerevisiae/metabolismo
Vinho/microbiologia
Adaptação Fisiológica
Vitis/metabolismo
Fermentação
Nitrogênio/metabolismo
-Saccharomyces cerevisiae/crescimento & desenvolvimento
Vitis/microbiologia
Responsável: CL1.1 - Biblioteca Central



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