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Id: biblio-1022493
Autor: Liu, Dongmei; Zhu, Hanyu; Chen, Yue; Zheng, Liesheng; Chen, Liguo; Ma, Aimin.
Título: Cloning and heterologous expression of a hydrophobin gene Ltr. hyd from the tiger milk mushroom Lentinus tuber-regium in yeast-like cells of Tremella fuciformis
Fonte: Electron. j. biotechnol;32:6-12, Mar. 2018. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China (NSFC).
Resumo: Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food products. Lentinus tuber-regium, known as tiger milk mushroom, is both an edible and medicinal sclerotium-producing mushroom. Up to now, the hydrophobins of L. tuber-regium have not been identified. Results: In this paper, a Class I hydrophobin gene, Ltr.hyd, was cloned from L. tuber-regium and expressed in the yeast-like cells of Tremella fuciformis mediated by Agrobacterium tumefaciens. The expression vector pGEH-GH was under the control of T. fuciformis glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter. The integration of Ltr.hyd into the genome of T. fuciformis was confirmed by PCR, Southern blot, fluorescence observation and quantitative real-time PCR (qRT-PCR). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that recombinant hydrophobin rLtr.HYD with an expected molecular mass of 13 kDa was extracted. The yield of rLtr.HYD was 0.66 mg/g dry weight. The emulsifying activity of rLtr.HYD was better than the typical food emulsifiers sodium caseinate and Tween 20. Conclusions: We evaluated the emulsifying property of hydrophobin Ltr.HYD, which can be potentially used as a food emulsifier.
Descritores: Basidiomycota/metabolismo
Proteínas Fúngicas/genética
Lentinula/genética
Lentinula/metabolismo
-Transformação Genética
Basidiomycota/enzimologia
Leveduras
Proteínas Fúngicas/metabolismo
Southern Blotting
Clonagem Molecular
Agrobacterium tumefaciens/metabolismo
Análise de Sequência
Emulsificantes
Eletroforese em Gel de Poliacrilamida
Reação em Cadeia da Polimerase em Tempo Real
Gliceraldeído-3-Fosfato Desidrogenases/metabolismo
Microscopia de Fluorescência
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-988465
Autor: Flórez Sampedro, Laura; Zapata, Wildeman; Orozco, Lina P; Mejía, Amanda I; Arboleda, Carolina; Rugeles, María T.
Título: In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp / Actividad anti-VIH-1 in vitro del extracto enzimático enriquecido con lacasa producido por los hongos Ganoderma sp. y Lentinus sp
Fonte: Vitae (Medellín);23(2):109-118, 2016. Ilustraciones.
Idioma: en.
Resumo: Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein ­GFP­ and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.

Antecedentes: Los compuestos naturales son una buena fuente para el desarrollo de fármacos antirretrovirales con baja citotoxicidad. La enzima lacasa, producida por hongos del género Ganoderma sp. y Lentinus sp., inhibe la transcriptasa reversa (TR) del virus de la inmunodeficiencia humana tipo 1 (VIH-1), en modelos in vitro, libres de células. Objetivos: En este estudio se evaluó la actividad anti-VIH-1 del extracto enzimático (EE) enriquecido con lacasa, producida por dos especies nativas de hongos de los mismos géneros en un modelo in vitro de cultivo celular. Métodos: La inhibición de la replicación viral se realizó usando la línea celular U373-MAGI infectada con virus recombinantes en la presencia/ ausencia del EE y 48 hpi, el porcentaje de células infectadas se evaluó mediante citometría de flujo para GFP y ELISA para p24. La inhibición de la TR se determinó mediante la cuantificación de los productos tempranos y tardíos de la transcripción reversa utilizando una PCR cuantitativa. Resultados: El EE de Ganoderma sp. y Lentinus sp. inhibió la replicación del VIH-1 entre el 80 y 90% y disminuyó la producción de transcriptos tempranos y tardíos entre el 55,5% -91,3% y 82,1% -93,6%, respectivamente. El EE de Lentinus sp. mostró el mejor índice de selectividad (IS: 8.3). Conclusiones: Estos resultados sugieren el potencial anti-VIH-1 del EE para la exploración de una terapia alternativa contra la infección por el VIH-1.
Descritores: Antivirais
HIV-1
-Produtos Biológicos
Lentinula
Ganoderma
Lacase
Limites: Seres Humanos
Tipo de Publ: Artigo Clássico
Responsável: CO56.3 - Biblioteca


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Romanha, A. J
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Id: lil-564126
Autor: Souza-Fagundes, E. M; Cota, B. B; Rosa, L. H; Romanha, A. J; Corrêa-Oliveira, R; Rosa, C. A; Zani, C. L; Teixeira-Carvalho, A; Martins-Filho, O. A.
Título: In vitro activity of hypnophilin from Lentinus strigosus: a potential prototype for Chagas disease and leishmaniasis chemotherapy
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(11):1054-1061, Nov. 2010. ilus, tab.
Idioma: en.
Resumo: Hypnophilin and panepoxydone, terpenoids isolated from Lentinus strigosus, have significant inhibitory activity onTrypanosoma cruzi trypanothione reductase (TR). Although they have similar TR inhibitory activity at 10 μg/mL (40.3 μM and 47.6 μM for hypnophilin and panepoxydone, respectively; ~100 percent), hypnophilin has a slightly greater inhibitory activity (~71 percent) on T. cruzi amastigote (AMA) growth in vitro as well as on in vitro phytohemagglutinin (PHA)-induced peripheral blood mononuclear (PBMC) proliferation (~70 percent) compared to panepoxydone (69 percent AMA inhibition and 91 percent PBMC inhibition). Hypnophilin and panepoxydone at 1.25 μg/mL had 67 percent inhibitory activity onLeishmania (Leishmania) amazonensis amastigote-like (AMA-like) growth in vitro. The panepoxydone activity was accompanied by a significant inhibitory effect on PHA-induced PBMC proliferation, suggesting a cytotoxic action. Moreover, incubation of human PBMC with panepoxydone reduced the percentage of CD16+ and CD14+ cells and down-regulated CD19+, CD4+ and CD8+ cells, while hypnophilin did not alter any of the phenotypes analyzed. These data indicate that hypnophilin may be considered to be a prototype for the design of drugs for the chemotherapy of diseases caused by Trypanosomatidae.
Descritores: Antiprotozoários/farmacologia
Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia
Leishmania/efeitos dos fármacos
Lentinula/química
Extratos Vegetais/farmacologia
Sesquiterpenos/farmacologia
Trypanosoma cruzi/efeitos dos fármacos
-Antígenos CD/efeitos dos fármacos
Compostos Bicíclicos Heterocíclicos com Pontes/isolamento & purificação
Proliferação Celular/efeitos dos fármacos
Desenho de Drogas
Leucócitos Mononucleares/efeitos dos fármacos
Sesquiterpenos/isolamento & purificação
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  4 / 10 LILACS  
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Romanha, Alvaro José
Rosa, Carlos Augusto
Zani, Carlos Leomar
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Id: lil-485218
Autor: Cota, Betania Barros; Rosa, Luiz Henrique; Fagundes, Elaine Maria Souza; Martins-Filho, Olindo Assis; Correa-Oliveira, Rodrigo; Romanha, Alvaro José; Rosa, Carlos Augusto; Zani, Carlos Leomar.
Título: A potent trypanocidal component from the fungus Lentinus strigosus inhibits trypanothione reductase and modulates PBMC proliferation
Fonte: Mem. Inst. Oswaldo Cruz;103(3):263-270, May 2008. ilus, graf, tab.
Idioma: en.
Resumo: The fungus Lentinus strigosus (Pegler 1983) (Polyporaceae, basidiomycete) was selected in a screen for inhibitory activity on Trypanosoma cruzi trypanothione reductase (TR). The crude extract of L. strigosus was able to completely inhibit TR at 20 µg/ml. Two triquinane sesquiterpenoids (dihydrohypnophilin and hypnophilin), in addition to two panepoxydol derivatives (neopanepoxydol and panepoxydone), were isolated using a bioassay-guided fractionation protocol. Hypnophilin and panepoxydone displayed IC50 values of 0.8 and 38.9 µM in the TR assay, respectively, while the other two compounds were inactive. The activity of hypnophilin was confirmed in a secondary assay with the intracellular amastigote forms of T. cruzi, in which it presented an IC50 value of 2.5 µ M. Quantitative flow cytometry experiments demonstrated that hypnophilin at 4 µM also reduced the proliferation of human peripheral blood monocluear cells (PBMC) stimulated with phytohemaglutinin, without any apparent interference on the viability of lymphocytes and monocytes. As the host immune response plays a pivotal role in the adverse events triggered by antigen release during treatment with trypanocidal drugs, the ability of hypnophilin to kill the intracellular forms of T. cruzi while modulating human PBMC proliferation suggests that this terpenoid may be a promising prototype for the development of new chemotherapeutical agents for Chagas disease.
Descritores: Proliferação Celular/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Lentinula/química
NADH NADPH Oxirredutases/antagonistas & inibidores
Tripanossomicidas/farmacologia
Trypanosoma cruzi/efeitos dos fármacos
-Inibidores Enzimáticos/isolamento & purificação
Citometria de Fluxo
INHIBITORY CONCENTRATION ACADEMIES AND INSTITUTES
Linfócitos/efeitos dos fármacos
Monócitos/efeitos dos fármacos
Tripanossomicidas/isolamento & purificação
Trypanosoma cruzi/enzimologia
Limites: Animais
Bovinos
Seres Humanos
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-421259
Autor: Granda R., Diana; Mejía G., Amanda Inés; Jiménez T., Gloria.
Título: Utilización de residuos de plátano para la producción de metabolitos secundarios por fermentación en estado sólido con el hongo lentinus crinitus / Utilization of plantain waste for the production of secondary metabolites by solid substrate fermentation using the fungi lentinus crinitus
Fonte: Vitae (Medellín);12(2):13-20, mar. 2005-sept. 2005. ilus, tab, graf.
Idioma: es.
Resumo: En este articulo se presenta la producción de metabolitos secundarios de interés farmacéutico y alimentario obtenidos de los residuos del cultivo de plátano al emplearse como sustrato en un proceso de fermentación en estado sólido con el hongo de la podredumbre de la madera Lentinus crinitus.La producción de metabolitos se determina durante el crecimiento del microorganismo por un periodo de 21 días, en un sistema de fermentación conformado por 7 combinaciones de sustrato: tallo-fruto, hojas-fruto, hojas-tallo, hojas, tallo, fruto y hojas-tallo-fruto. La mejor combinación para la producción de las enzimas ligninoperoxidasa (LiP) y manganesoperoxidasa (MnP) es la conformada por hojas-tallo de plátano Musa paradisiaca. Esto es importante para aprovechar el enorme potencial que tienen estos residuos para la producción de enzimas peroxidasas, las cuales tienen muchas aplicaciones. También es importante destacar que anteriormente no se tienen reportes en la literatura a cerca de la producción de la enzima ligninoperoxidasa por el hongo Lentinus crinitus. En los sistemas donde se obtiene actividad enzimática: (tallo-fruto, hojas-fruto, hojas-tallo y hojas-tallo-fruto) se evalúa la presencia de los compuestos aromáticos ácido ferúlico, vainilla, ácido vainillínico y eugenol en los días 11, 16 y 21. Los análisis se hacen por cromatografía líquida de alta eficienia (HPLC). En los sistemas de mayor actividad enzimática conformados por la mezcla de hojas-tallos, los productos aromáticos de interés se detectaron en muy baja concentración. En el sistema conformado por hojas-frutos donde solo se destaca la producción de la enzima LiP, se encuentra ácido ferúlico, vainilla, ácido vainillínico y eugenol en cantidades importantes durante el día 16
Descritores: Eugenol
Lentinula/isolamento & purificação
Lentinula/metabolismo
Resíduos Sólidos
Uso de Resíduos Sólidos
Zingiberales
Responsável: CO56.3 - Biblioteca


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Id: lil-316970
Autor: Ishikawa, Noemia Kazue; Kasuya, Maria Catarina Megumi; Vanetti, Maria Cristina Dantas.
Título: Antibacterial activity of Lentinula edodes grown in liquid medium
Fonte: Braz. j. microbiol;32(3):206-210, July-Sept., 2001. tab, graf.
Idioma: en.
Resumo: The antibacterial activity of 35 isolates of Lentinula edodes, a shiitake mushroom, against Bacillus subtilis was evaluated by diffusion technique in agar with a semi-solid overlay. All isolates inhibited B. subtilis and the isolate Le1 promoted the formation of the largest inhibition zone. L. edodes Le1 also presented antibacterial activity against foodborne pathogens and food contaminant bacteria, particularly Gram-positive species. The antibacterial activity of the culture filtrate after 18-25 days of cultivation of L. edodes in broth at 25§C was high. The inhibitory activity was observed only in the organic layer when the culture filtrate was partitioned between ethyl acetate and water, suggesting that the inhibitory substances have low polarity. The silica gel thin-layer zone at Rf values of 0.63-0.80, developed in chloroform - acetone - ethyl acetate - methanol = 40:5:5:2, was responsible for the antibacterial activity against B. subtilis. The inhibitory activity of L. edodes was detectable in the culture filtrate after heat treatment at 100§C for 10 min and after storage at 4§C for 120 days.
Descritores: Antígenos de Bactérias/imunologia
Antígenos de Bactérias/isolamento & purificação
Bactérias/patogenicidade
Técnicas In Vitro
Lentinula
-Cromatografia em Camada Delgada
Meios de Cultura
Tipo de Publ: Revisão de Integridade Científica
Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-316965
Autor: Paccola, Edneia A de Souza; Maki, Cristina Sayuri; Nobrega, Gisele M A de; Paccola-Meirelles, Luzia Doretto.
Título: Antagonistic effect of edible mushroom extract on Candida Albicans growth
Fonte: Braz. j. microbiol;32(3):176-178, July-Sept., 2001. tab, graf.
Idioma: en.
Resumo: Five species of edible mushrooms, Lentinula edodes, Pleurotus ostreatus, Pholiota nameko, Macrolepiota bonaerensis and Agaricus blazei, were tested for their potential to inhibit the in vitro growth of the pathogenic yeast Candida albicans. Only L. edodes had a fungistatic effect on this human pathogen. The inhibitory compound was produced intra and extracellularly in submersed L. edodes culture, and was also present in fresh and dehydrated mushroom basidiocarps. The fungistatic compound was heat sensitive and lost activity after 72 hours.
Descritores: Candida albicans
Candidíase/diagnóstico
Candidíase/enzimologia
Fungos
Técnicas In Vitro
Lentinula
-Cultura
Sintomas Patogenéticos
Tipo de Publ: Relatório Técnico
Relatos de Casos
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-316964
Autor: Maki, Cristina Sayuri; Teixeira, Flavia França; Paiva, Edilson; Paccola-Meirelles, Luzia Doretto.
Título: Analyses of genetic variability in Lentinula Edodes through Mycelia responses to different abiotic conditions and RAPD molecular markers
Fonte: Braz. j. microbiol;32(3):170-175, July-Sept., 2001. ilus, tab, graf.
Idioma: en.
Resumo: The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37§C) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28§C. The water content was lower in strains grown at 37§C. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering based on similarity coefficients allowed the separation of strain in two groups with different geographic origins.
Descritores: Fungos
Técnicas In Vitro
Lentinula
-Cultura
Linhagem
Tipo de Publ: Relatório Técnico
Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-297667
Autor: Sasaki, Selma H; Linhares, Rosa E. C; Nozawa, Carlos M; Montalván, Ricardo; Paccola-Meirelles, Luzia D.
Título: Strains of lentinula edodes suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of vesicular stomatitis virus
Fonte: Braz. j. microbiol;32(1):52-5, Jan.-Mar. 2001. tab.
Idioma: en.
Resumo: Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal pathogenic virus and filamentous fungi.
Descritores: Fungos
Lentinula
Vírus da Estomatite Vesicular Indiana
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Correia, Marcos José
Cavalcanti, Maria Auxiliadora de Queiroz
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Id: lil-251738
Autor: Correia, Marcos José; Pereira Junior, José Antônio de Souza; Santos, Jefferson Cunha dos; Cavalcanti, Maria Auxiliadora de Queiroz.
Título: Use of remazol blue dyed avicel for the determination of cellullolytic activity in basidiomycetes
Fonte: Rev. microbiol;29(4):286-8, out.-dez. 1998. graf.
Idioma: en.
Resumo: A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes in discussed. Refinaments were introduced in a simple method for qunatitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by enzimatic hydrolysis. Modification in Avicel-dye preparation were enhanced and a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maxmum wavelenght for RBBR (595 nm).
Descritores: Basidiomycota/metabolismo
Corantes
Técnicas In Vitro
-Celulase/análise
Lentinula/metabolismo
Pleurotus/metabolismo
Espectrofotômetros
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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