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Pesquisa : B01.300.179.120.760.649 [Categoria DeCS]
Referências encontradas : 9 [refinar]
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Texto completo SciELO Brasil
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Id: lil-727038
Autor: Rani, Babita; Kumar, Vivek; Singh, Jagvijay; Bisht, Sandeep; Teotia, Priyanku; Sharma, Shivesh; Kela, Ritu.
Título: Bioremediation of dyes by fungi isolated from contaminated dye effluent sites for bio-usability
Fonte: Braz. j. microbiol;45(3):1055-1063, July-Sept. 2014. ilus, graf.
Idioma: en.
Resumo: Biodegradation and detoxification of dyes, Malachite green, Nigrosin and Basic fuchsin have been carried out using two fungal isolates Aspergillus niger, and Phanerochaete chrysosporium, isolated from dye effluent soil. Three methods were selected for biodegradation, viz. agar overlay and liquid media methods; stationary and shaking conditions at 25 °C. Aspergillus niger recorded maximum decolorization of the dye Basic fuchsin (81.85%) followed by Nigrosin (77.47%), Malachite green (72.77%) and dye mixture (33.08%) under shaking condition. Whereas, P. chrysosporium recorded decolorization to the maximum with the Nigrosin (90.15%) followed by Basic fuchsin (89.8%), Malachite green (83.25%) and mixture (78.4%). The selected fungal strains performed better under shaking conditions compared to stationary method; moreover the inoculation of fungus also brought the pH of the dye solutions to neutral from acidic. Seed germination bioassay study exhibited that when inoculated dye solutions were used, seed showed germination while uninoculated dyes inhibited germination even after four days of observation. Similarly, microbial growth was also inhibited by uninoculated dyes. The excellent performance of A. niger and P. chrysporium in the biodegradation of textile dyes of different chemical structures suggests and reinforces the potential of these fungi for environmental decontamination.
Descritores: Aspergillus niger/metabolismo
Biodegradação Ambiental
Biotransformação
Corantes/metabolismo
Phanerochaete/metabolismo
Microbiologia do Solo
-Compostos de Anilina/metabolismo
Aspergillus niger/crescimento & desenvolvimento
Aspergillus niger/isolamento & purificação
Concentração de Íons de Hidrogênio
Resíduos Industriais
Phanerochaete/crescimento & desenvolvimento
Phanerochaete/isolamento & purificação
Corantes de Rosanilina/metabolismo
Temperatura Ambiente
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-626734
Autor: Barham, Feiruz; Roman, Patricia; Bull, Paulina.
Título: The PcACE1 transcription factor from Phanerochaete chrysosporium contains a Cys- and Ser-rich transactivation domain
Fonte: Biol. Res;44(4):351-355, 2011. ilus, tab.
Idioma: en.
Projeto: FONDECYT; . VRI.
Resumo: Transcription factor Ace1 from Saccharomyces cerevisiae regulates the expression of target genes when the copper concentration reaches 200 ìÌ levels. We are studying the ortholog of Ace1 from fungus Phanerochaete chrysosporium PcACE1, isolated by complementation in yeast. In this report we show the localization of the transactivation region of PcACE1. Different PcACE1 fragments were ligated in frame to the GAL4 DNA-binding domain by site-directed mutagenesis in a suitable yeast expression vector. Transformation of an appropriate Saccharomyces cerevisiae strain was used as host. This strain contains the fusion GAL1:lacZ in its genome under the control of promoter sequences recognized by GAL4. Finally, we measured â-galactosidase activity in each yeast clone. The activation of the reporter gene is proportional to the transactivation capacity of the transcription factor PcACE1. The results obtained indicate that PcACE1 transactivation domain is located in the carboxy terminal half and contains an array of cysteines in the form of Cys-X-Cys and Cys-X2-Cys and a 60% of Ser. Therefore, these results show that this type of Cys motif can function as transcription activating domain not only in transcription factors that respond to minimal copper concentrations but also in those that respond to high copper concentrations. This is the first transactivation domain reported in a basidiomycete fungus.
Descritores: Regulação Fúngica da Expressão Gênica/genética
Phanerochaete/genética
Fatores de Transcrição/genética
Transcrição Genética/genética
Ativação Transcricional/genética
-Sequência de Bases
Dados de Sequência Molecular
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: lil-531921
Autor: Alaoui, Sanae Mdaghri; Merzouki, Mohamed; Penninckx, Michel J; Benlemlih, Mohamed.
Título: Relationship between cultivation mode of white rot fungi and their efficiency for olive oil mill wastewaters treatment
Fonte: Electron. j. biotechnol;11(4):13-14, Oct. 2008. ilus, tab.
Idioma: en.
Resumo: Four white rot fungi (WRF) strains, Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona and Pycnoporus coccineus, were tested for efficiency of treatment of Olive Oil mill wastewaters (OOMW) in relation with their cultivation mode, i.e. under the form of free mycelium, mycelium immobilized in alginate beads and solid state cultivation on Petri dishes. Study of biodegradation of phenolic compounds, chemical oxygen demand (COD) decrease and decolourisation of OOMW have shown that Coriolopsis polyzona and Pycnoporus coccineus degradation performances were apparently only slightly affected by the cell cultivation procedures experienced here. In contrast, Phanerochaete chrysosporium and Trametes versicolor showed respectively marked preferences for solid state and alginate immobilisation procedures. Both mono and polyphenolics were reduced to different extent during incubation depending on the strain, as shown by gel filtration analysis. Final pH obtained after fungal treatment of the OOMW based medium (initial pH of 5.0) was measured in order to evaluate the possibility of releasing friendly the treated wastewater in the environment. Laboratory studies as reported here may be useful for orienting the choice of a strain for treating pollution by OOMW in a particular real situation.
Descritores: Basidiomycota/enzimologia
Fungos/enzimologia
Phanerochaete/enzimologia
Tratamento de Águas Residuárias/métodos
-Alginatos
Biorremediação (Saúde Ambiental)/métodos
Peroxidases
Gorduras Vegetais
Responsável: CL1.1 - Biblioteca Central


  4 / 9 LILACS  
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Id: lil-502201
Autor: Arboleda, Carolina; Segura, Freimar; Mejía, Amanda Inés.
Título: Enzymatic transformation of crystalline structure of copolymer poly (ethylene-co-vinyl alcohol) (EVOH)
Fonte: Vitae (Medellín);14(1):25-30, ene.-jun. 2007. graf.
Idioma: en.
Resumo: Changes in the crystalline structure of the copolymer poly(ethylene-co-vinyl alcohol) (EVOH) that occurs during the enzymatic fermentation system with the fungus Phanerochaete chrysosoporium were studied. This fungus has a powerful group of oxidative enzymes that had been used for several processes of environmental pollutants degradation. In order to use the enzymatic system of the fungus to improve degradation, films of the copolymer are inoculated and maintained in a solid state fermentation (SSF) system using as substrate a mixture of agroindustrial corn wastes with an initial humidity of 90 por ciento, during forty days. Analyses of the films were made at different times of fermentation. The effect on the chemical structure of the polymer was studied by using FTIR-spectroscopy with attenuated total reflection (ATR) and changes in the crystalline structure were determined by differential scan calorimetry DSC and X-ray diffraction technique. The lignin peroxidase (LiP) activity was followed by UV-spectrophotometry. We concluded that after 20 days of fermentation the copolymer has lost 50 percent of the initial crystalline structure.
Descritores: Peroxidase
Phanerochaete
Responsável: CO56.3 - Biblioteca


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Texto completo SciELO Chile
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Id: lil-456599
Autor: Polanco, Rubén; Canessa, Paulo; Rivas, Alexis; Larrondo, Luis F; Lobos, Sergio; Vicuña, Rafael.
Título: Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
Fonte: Biol. Res;39(4):641-648, 2006. ilus, graf.
Idioma: en.
Projeto: FONDECYT-Chile; . Millennium Institute for Fundamental and Applied Biology.
Resumo: In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes.
Descritores: Proteínas de Ligação a DNA/genética
Proteínas Fúngicas/genética
Phanerochaete/genética
Proteínas de Saccharomyces cerevisiae/genética
Fatores de Transcrição/genética
-Northern Blotting
Clonagem Molecular
Cobre/farmacologia
DNA Complementar
Regulação Fúngica da Expressão Gênica
Modelos Genéticos
Phanerochaete/efeitos dos fármacos
RNA Mensageiro/análise
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 9 LILACS  
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Id: lil-415333
Autor: Segura S., Freimar; Mejía G., Amanda; Jiménez T., Gloria.
Título: Obtención de metabolitos secundarios en cultivos sumergidos del phanerochaete chrysosporium con adición de sustratos poliméricos / Obtaining of secondary metabolites in phanerochaete chrysosporium submerged cultures by addition of polymeric substrates
Fonte: Vitae (Medellín);11(2):35-41, mar.-sept. 2004. graf.
Idioma: es.
Resumo: La producción de la enzima ligninoperoxidasa -LiP- y de metabolitos de interés como la vainilla, son estudiados adicionando los polímeros aserrín de pino (AP) y polivinilalcohol (PVOH) a cultivos sumergidos del Phanerochaete chrysosporium, usando bioreactores con agitación orbital y con agitación neumática. Los medios que contienen AP y alcohol veratrílico, en agitadores orbitales, presentan una correlación directa entre la producción de LiP y de vainilla, independiente de si tienen PVOH. En los medios con PVOH se obtiene mayor cantidad de LiP que en los que no lo contienen. En los experimentos con agitación neumática se observa ácido vainillínico, lo que implica que la aireación causa oxidación de la vainilla haciendo inapropiado éste tipo de bioreactor
Descritores: Fungos
Phanerochaete
Responsável: CO56.3 - Biblioteca


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Id: lil-353598
Autor: Segura S., Freimar; Mendoza., Nancy; Mehjia G., Amanda.
Título: Ensayo de diferentes tipos de biorreactores para escalar la producción de la enzima ligninoperoxidasa en cultivos sumergidos de Phanerchaete Chrysosporium / Assay of different bioreactors types to scale the production of the enzime ligninoperoxidasa in submerged cultures of Phanerochaete Chrysosporium
Fonte: Vitae (Medellín);9(2):17-26, mar. 2002-sept. 2002. ilus, tab, graf.
Idioma: es.
Resumo: El objetivo de éste trabajo es diseñar un bioreactor adecuado para escalar la producción de la enzima lignina peroxídasa (LiP) en cultivos sumergidos del hongo Phanerocahete chrysosporium. Se estudiaron tres bioreactores con diferentes características. El primer biorreactor es de tipo tanque con agitación orbital y neumática; los otros dos son tipo columna de burbujeo de una sola etapa que difieren en su material de elaboración (vidrio polipropileno). La producción de la LiP se logró en los tres fermentadores, pero el sistema que mostró la mayor rata de producción de LiP, es el biorreactor de vidrio tipo columna
Descritores: Reatores Biológicos
Enzimas
Fungos
Phanerochaete
Responsável: CO56.3 - Biblioteca


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Texto completo SciELO Brasil
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Id: lil-342092
Autor: Uysal, Handan; Aydogan, M. Nuri; Algur, O. Faruk.
Título: Effect of single cell protein as a protein source in Drosophila culture
Fonte: Braz. j. microbiol;33(4):314-317, Oct.-Dec. 2002. ilus, tab.
Idioma: en.
Resumo: The effects of biomass of Phanerochaete chrysosporium at various concentrations (1; 2.5; 5; 10; 25; 50; 75 and 100 percent) on the growth of Drosophila melanogaster have been investigated. Biomass was used as a protein source instead of corn flour in Standard Drosophila Medium (SDM). It was seen that it causes increments in both body size of larvae and the numbers of offsprings, specially at higher concentrations (50, 75 and 100 percent) at the application of 100 percent concentration, metamorphosis accelerated and was completed one day before the control. There were phenotypic abnormalities in both control (0.64 percent) and applications with low concentrations (0.02-0.19 percent), while they were not observed at the application of 100 percent concentration.
Descritores: Biomassa
Drosophila
Técnicas In Vitro
Phanerochaete
Medidas de Toxicidade
-Meios de Cultura
Métodos
Tipo de Publ: Revisão de Integridade Científica
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Texto completo SciELO Venezuela
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Id: lil-341080
Autor: Roldán Carrillo, Teresa; Rodríguez Vázquez, Refugio; Vázquez Torres, Humberto; Cardoso Martínez, Judith; Torres Domínguez, Angeles.
Título: Remoción de estireno por phanerochaete chrysosporium: en cultivo líquido / Styrene removal by phanerochaete chrysosporium: in liquid culture
Fonte: Interciencia;26(12):611-614, dic. 2001. ilus, graf.
Idioma: es.
Resumo: El objetivo de este trabajo fue demostrar si un hongo ligninolítico, Phanerochaete chrysosporium, podría tener la capacidad de 1) tolerar altas concentraciones de estireno y 2) remover el estireno en un cultivo líquido. Los resultados mostraron que el hongo fue capaz de crecer a concentraciones de estireno bajas (200mg/l) y relativamente altas (1500mg/l). se mostró que 40 y 46 por ciento de estireno fue biodegradado, respectivamente, en 35 días. Sin embargo, 30 y 53 por ciento, fue removido por volatilización. La detección de la actividad enzimatica de lignina peroxidasa en el cultivo del hongo con estireno a las dos concentraciones, quizas implique su participación en la biodegradación de este compuesto. El ácido benzóico fue detectado en el cultivo como producto de la biodegradación
Descritores: Chrysosporium
Meios de Cultura
Enzimas
Fungos
Phanerochaete
Estireno
-México
Ciência
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha



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