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Id: biblio-1253026
Autor: Silva Ferreira, Isabela Natália; Montero Rodríguez, Dayana; Campos-Takaki, Galba Maria; Silva Andrade, Rosileide Fontenele da.
Título: Biosurfactant and bioemulsifier as promising molecules produced by Mucor hiemalis isolated from Caatinga soil
Fonte: Electron. j. biotechnol;47:51-58, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico); . FACEPE (Fundação de Amparo à Ciência e Tecnologia de Pernambuco).
Resumo: BACKGROUND: The present study describes the production of biosurfactant (BS) and emulsifier (BE) by the filamentous fungus Mucor hiemalis UCP 0039, as well as the characterization and stability of the both biomolecules for environmental or industrial applications. RESULTS: Biosurfactants and bioemulsifiers are amphiphilic compounds and are produced as extracellular molecules. The results showed that bioproduct obtained by shaker condition reduced the water surface tension of 72 to 32 mN/m and reached an emulsification index of 96%, while the static cultivation resulted in a biomolecule with a surface tension of 40 mN/m and an emulsification index of 96%, suggesting the production of a biosurfactant and bioemulsifier, respectively. The compounds showed glycolipid nature but the biosurfactant presented cationic charge, while the bioemulsifier, anionic charge. Thus, the results confirmed that M. hiemalis produced two distinct biomolecules under different parameters and in the same culture medium. CONCLUSIONS: It is the first time that biosurfactant and emulsifier production has been described in the same medium and under different physical conditions by Mucor hiemalis. Both biomolecules showed thermal stability, as well as have significant effect on the viscosity of hydrophobic compounds, indicating the excellent potential for environmental safety or industrial applications to improve the efficiency of sustainable and economic technologies.
Descritores: Tensoativos/metabolismo
Emulsificantes/metabolismo
Mucor/metabolismo
-Solo
Tensoativos
Óleo de Soja
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-886788
Autor: SANTOS, GABRIEL F DOS; TAKAHASHI, JACQUELINE A.
Título: A New Acetylcholinesterase Inhibitor from Green Glycosylation of Trachyloban-19-oic Acid by Mucor plumbeus
Fonte: An. acad. bras. ciênc;89(3,supl):1961-1969, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The in vitro metabolism of a widespread natural product, trachyloban-19-oic acid (1), by the fungal species Mucor plumbeus was studied in a sucrose-yeast liquid medium. Two products were isolated, and their structures were determined by spectroscopic means as 7β-hydroxytrachyloban-19-oic acid (5) and trachyloban-19-O-β-D-glucopyranosyl ester (6). To the best of our knowledge, compound 6 is herein reported by the first time in the literature. These compounds were assayed for acetylcholinesterase inhibition along with some related compounds. Compound 6 showed the highest acetylcholinesterase inhibitory activity at 10000 µg/mL among the tested compounds, a result (92.89%) comparable to the activity of the positive control, galanthamine (94.21%). Therefore, biotransformation of the natural product 1 by M. plumbeus produced a novel compound with potential as a new lead to develop anti-Alzheimer medicines.
Descritores: Inibidores da Colinesterase/metabolismo
Meios de Cultura/química
Diterpenos/metabolismo
Mucor/metabolismo
-Glicosilação
Extratos Vegetais
Biotransformação
Inibidores da Colinesterase/química
Diterpenos/química
Responsável: BR1.1 - BIREME


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Id: biblio-1011281
Autor: Clementino, E. L; Sales, A. E; Cunha, M. N. C; Porto, A. L. F; Porto, T. S.
Título: Produção e purificação integrada de protease fibrinolítica de Mucor subtilissimus UCP 1262 / Integrated production and purification of fibrinolytic protease from Mucor subtilissimus UCP 1262
Fonte: Arq. bras. med. vet. zootec. (Online);71(2):553-562, mar.-abr. 2019. tab, ilus.
Idioma: pt.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Conselho nacional de Desenvolvimento Científico e Tecnológico; . Fundação de Amparo à Ciência e Tecnologia de Pernambuco.
Resumo: As enzimas fibrinolíticas podem ser obtidas de micro-organismos por meio de processos fermentativos. O presente trabalho teve como objetivo avaliar a produção e extração integrada da protease fibrinolítica de Mucor subtilissimus UCP 1262 usando sistema de duas fases aquosas (SDFA). O processo integrado foi realizado para avaliar a produção, partição e recuperação da protease fibrinolítica, segundo planejamento experimental 23, utilizando como variáveis independentes a massa molar do polietileno glicol (PEG), a concentração do PEG e a concentração do sulfato de sódio. A maior atividade fibrinolítica (15,40U/mL) foi obtida na fase rica em sulfato de sódio no ensaio composto por 10% de sal e 18% de PEG 8000 (g/mol). Recuperações superiores a 80% foram obtidas. A protease fibrinolítica apresentou pH ótimo 7,0, estabilidade entre os pH 6,0 e 8,5, temperatura ótima 50°C, sendo estável de 10°C a 50°C. A enzima foi classificada como uma serino protease, com massa molecular de 52kDa. Como resultado, o processo é notavelmente eficaz para pré-purificar a protease fibrinolítica com baixo custo e rapidez significativa. Quando comparada a outras técnicas de produção e purificação isoladas, a fermentação extrativa é um processo digno a ser substituto das etapas iniciais de separação convencionais.(AU)

Fibrinolytic enzymes can be obtained from microorganisms through fermentative processes. The study aimed to evaluate the fibrinolytic protease production and integrated extraction from Mucor subtilissimus UCP 1262 by extractive fermentation using Aqueous Two-Phase Systems (ATPS). The integrated process was carried out to assess the production, partition and fibrinolytic enzyme recovery, according to a 2 3 -experimental design, using as independent variables Polyethylene glycol (PEG) molar mass, PEG and sodium sulphate concentration, concentration. The highest fibrinolytic activity (15.40U/mL) was obtained in sodium sulfate rich phase in the assay comprising of 10% of salt and 18% of PEG 8000 (g/mol). Yield greater than 80% was obtained. The fibrinolytic protease presented optimum pH 7.0 and stability between pH 6.0 and 8.5, and optimum temperature 50°C, stable between 10°C to 50°C. The enzyme was classified as a serine-protease with 52kDa of molecular weight. As a result, the process is remarkably effective to pre-purify the fibrinolytic protease with a low cost and significantly faster processing time. When compared to other isolated production and purification techniques the extractive fermentation is worthy of being a candidate to replace the initial stages of conventional separation processes.(AU)
Descritores: Fibrina/antagonistas & inibidores
Fibrinolíticos/isolamento & purificação
Mucor/enzimologia
-Indução Enzimática
Fermentação
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1021550
Autor: Sharifyazd, Shabnam; Karimi, Keikhosro.
Título: Effects of fermentation conditions on valuable products of ethanolic fungus Mucor indicus
Fonte: Electron. j. biotechnol;30:77-82, nov. 2017. tab, graf.
Idioma: en.
Resumo: Background: Mucor indicus is a dimorphic fungus used in the production of ethanol, oil, protein, and glucosamine. It can ferment different pentoses and hexoses; however, the yields of products highly depend on the nutrients and cultivation conditions. In this study, the effects of different morphologic forms, cultivation time and temperature, presence or absence of oxygen, carbon sources, and concentration of nitrogen source on the products of M. indicus were investigated. Results: The fungus with all morphologies produced high yields of ethanol, in the range of 0.32­0.43 g/g, on glucose. However, the fungus with filamentous morphology produced higher amounts of oil, protein, phosphate, and glucosamine together with ethanol, compared with other morphologies. A higher amount of oil (0.145 g/g biomass) was produced at 28°C, while the best temperature for protein and glucosamine production was 32 and 37°C, respectively. Although ethanol was produced at a higher yield (0.44 g/g) under anaerobic conditions compared with aerobic conditions (yield of 0.41 g/g), aerobic cultivation resulted in higher yields of protein (0.51 g/g biomass), glucosamine (0.16 g/g alkali insoluble material, AIM), and phosphate (0.11 g/g AIM). Conclusions: It is not possible to have the maximum amounts of the products simultaneously. The fermentation conditions and composition of culture media determine the product yields. Carbon source type and the addition of nitrogen source are among the most influencing factors on the product yields. Moreover, all measured products were made with higher yields in cultivation on glucose, except glucosamine, which was produced with higher yields on xylose.
Descritores: Etanol/metabolismo
Mucor/metabolismo
-Temperatura
Fatores de Tempo
Óleos/metabolismo
Carbono/metabolismo
Biomassa
Aerobiose
Meios de Cultura
Fermentação
Glucosamina/metabolismo
Glucose
Anaerobiose
Nitrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1026503
Autor: Fischer, Ivan Herman; Fernandes Júnior, Flávio; Kano, Cristiaini; Donadelli, Alceu; Palharini, Maria Cecília de Arruda.
Título: Adubação nitrogenada sobre a ocorrência de doenças em pós-colheita do morango / Effect of nitrogen doses on the occurrence of postharvest diseases in strawberry
Fonte: Arq. Inst. Biol;82:1-5, 2015. tab, graf.
Idioma: pt.
Resumo: Avaliou-se o efeito de doses de nitrogênio, aplicadas em campo, sobre as doenças pós-colheita nos cultivares de morango Camino Real, Festival e Oso Grande. O nitrogênio, tendo como fonte a ureia, foi avaliado na dose recomendada total (100%), com base na recomendação oficial para a cultura, e mais 3 doses inferiores (83, 67 e 50% do recomendado), tanto no plantio como em cobertura. Os frutos foram colhidos e pesados com 75% da superfície de cor vermelho-brilhante. Doze frutos por parcela foram amostrados em 3 coletas a partir da segunda florada e avaliados quanto à incidência das doenças durante 5 dias de armazenamento a 25°C e 85% de umidade relativa. A identificação das doenças foi feita diariamente com base nos sintomas e sinais dos patógenos. Não foi observado efeito das doses de nitrogênio na produção de frutos e na incidência das doenças, expressa em área abaixo da curva de progresso da doença (AACPD). A maior produção foi observada nos cultivares Festival e Oso Grande. A incidência total de doenças não diferiu significativamente entre os cultivares. O mofo cinzento foi a principal doença, com incidência superior a 30%, seguido da podridão mole de Rhizopus e podridão de levedura.(AU)

This study evaluated the effect of nitrogen doses on postharvest diseases of strawberry cultivars Camino Real, Festival and Oso Grande. The nitrogen in urea formulation was evaluated at the recommended dose (100%) based on the official recommendation to the culture, and 3 lower doses (83, 67 and 50% of recommended), considering the fertilization at planting and cover applications. Fruits were harvested and weighed at 75% of its surface with a bright red color. Twelve fruits per plot were sampled in 3 collections from the second flowering and assessed for disease incidence during five days of storage at 25°C and 85% relative humidity. The identification of disease was made daily, based on symptoms and pathogen signs. There was no effect of nitrogen doses on yield and incidence of postharvest diseases, expressed as area under the disease progress curve (AUDPC). Increased average production was observed in cultivars Festival and Oso Grand. The overall incidence of disease among the cultivars did not differ significantly. Gray mold was the main disease, affecting more than 30% of fruits, followed by Rhizopus and yeast rot.(AU)
Descritores: Botrytis
Fragaria
Fungos
Mucor
Nitrogênio
-Doenças das Plantas
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-868816
Autor: García, Mariajosé; Contreras, Verónica; Rojas, Bernardita; Cruz Choappa, Rodrigo.
Título: Mucormicosis rinosinusal por mucor hiemalis en una paciente con leucemia mieloide aguda / Rhinosinusal mucormycosis by mucor hiemalis in a patient with acute myeloid leukemia
Fonte: Bol. micol. (Valparaiso En linea);31(2):51-58, dic. 2016. ilus.
Idioma: es.
Resumo: Se reporta un caso clínico de una paciente femenina de 41 años, con antecedentes de leucemia mieloide aguda (LMA) en remisión. Estudiada por hematología, se confirmó recaída de LMA M4. Se inició quimioterapia. La paciente evolucionó con pancitopenia severa. Presentó dos episodios de neutropenia febril, el primero fue asociado a un absceso glúteo que se trató con antibacterianos, y el segundo a compromiso rinosinusal y úlcera necrótica de punta nasal, columela, tabique, cornete inferior izquierdo y paladar duro. Debido a la clínica e imá- genes radiológicas, se sospechó mucormicosis, por lo que se realizó cirugía con debridación extensa y se inició tratamiento antimicótico con anfotericina B desoxicolato. El cultivo de tejido informó abundante desarrollo de Mucor hiemalis. Se mantuvo pancitopénica durante aproximadamente un mes, siendo diariamente evaluada por un equipo multidisciplinario. Se hicieron varios aseos quirúrgicos, en el último se encontró tejido vital. La paciente completó diez días con anfotericina B desoxicolato y posteriormente se hizo traslape a posaconazol oral. Se realizó mielograma de control que evidenció remisión completa de recaída de LMA. Se dio de alta a su domicilio al día 40 de hospitalización, con hemograma adecuado y tratamiento oral con posaconazol para completar 6 semanas en total.

We report a case of a 41-years-old female patient with a history of acute myeloid leukemia (AML) in remission. Hematology studies confirmed relapse of AML M4. Chemotherapy was started. The patient developed severe pancytopenia. She presented two episodes of febrile neutropenia, the first one was associated with a gluteal abscess that was treated with antibacterials, and the second to rhinosinusal involvement and necrotic ulcer of nasal tip, columella, septum, left inferior turbinate and hard palate. Due to clinical and radiological imaging, mucormycosis was suspected, so surgery was performed with extensive debridement and antifungal treatment with amphotericin B deoxicholate was initiated. Tissue culture reported abundant development of Mucor hiemalis. She remained pancytopenic for approximately one month, being evaluated daily by a multidisciplinary team. Several surgical were made, finding vital tissue in the last perform. The patient completed ten days with amphotericin B deoxicholate and later was overlapped to oral posaconazole. A control myelogram was performed, showing complete remission of AML. She was discharged to her home at day 40 of hospitalization, with adequate blood count and oral treatment with posaconazole to complete 6 weeks in total.
Descritores: Anfotericina B
Neutropenia Febril Induzida por Quimioterapia
Leucemia Mieloide Aguda/complicações
Mucor/patogenicidade
Mucormicose/cirurgia
Mucormicose/diagnóstico por imagem
Mucormicose/tratamento farmacológico
Seios Paranasais/cirurgia
Seios Paranasais/microbiologia
-Antifúngicos
Desbridamento/métodos
Espectroscopia de Ressonância Magnética/métodos
Doenças Hematológicas
Fungos/patogenicidade
Fatores de Risco
Tomografia Computadorizada Espiral/métodos
Limites: Humanos
Adulto
Feminino
Tipo de Publ: Relatos de Casos
Responsável: CL2.1 - Biblioteca de Medicina


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Id: lil-634472
Autor: Fuselli, S.R.; Filsinger, B.; Fritz, R.; Yeannes, M.I..
Título: Estudio microbiológico de ajo(Allium sativum L.) y cebolla (Allium cepa L.) deshidratados / Microbiological study of garlic (Allium sativum L.) and onion (Allium cepaL.) dehydrated
Fonte: Rev. argent. microbiol;36(3):139-144, jul.-sep. 2004. ilus, tab.
Idioma: es.
Resumo: En este trabajo se efectuó un estudio microbiológico durante la deshidratación y el almacenamiento de ajo (Allium sativum L.) y de cebolla (Allium cepa L.). Al ajo se le efectuó un proceso de escaldado y a la cebolla un salmuereado previo a la deshidratación. En las materias primas los recuentos promedio expresados en UFC/g fueron: bacterias aerobias mesófilas entre 1,2 x 102y 1,6 x 103, mohos y levaduras entre 60 y 1,6 x 103, Lactobacillus spp. y Leuconostocmesenteroidessubesp. mesenteroides entre 10 y 50.Se identificaron: Penicillium spp., Monilia spp. Lactobacillus brevis,Leuconostoc mesenteroides subesp. mesenteroides y levaduras en ajo; Mucor spp., Penicillium spp., Monilia spp., Lactobacillus brevis y levaduras en dos tipos de cebolla. Leuconostoc mesenteroides subesp. mesenteroides sólo se detectó en un tipo de cebolla. En ajos deshidratados y durante el almacenamiento se hallaron Penicillium spp., Monilia spp., Lactobacillus brevis y levaduras mientras que los mismos con escaldado no presentaron desarrollo. Mucor spp., Penicillium spp., Monilia spp. y Lactobacillus brevis se identificaron en los dos tipos de cebolla deshidratadas. Con la incorporación del salmuereado la microflora se redujo significativamente hallándose solamente Penicillium spp. La utilización de barreras adicionales de control microbiano tales como escaldado y salmuereado produce un aporte importante a la estabilidad microbiológica de los productos.

A microbiological study during the process and the storage of garlic (Allium sativum L.) and onion (Allium cepa L.) dehydrated, with the additional barriers of blanching or brine immersion, was made. In all raw materials the average counts of aerobic mesophilic bacteria expressed in CFU/g ranged from 1.2 x 102to 1.6 x 103, molds and yeasts from 60 to 1.6 x 103, Lactobacillus spp. and Leuconostocmesenteroides subsp. mesenteroides between 10 and 50. Microorganisms identified were Penicillium spp., Monilia spp., Lactobacillus brevis, Leuconostoc mesenteroides subsp. mesenteroides and yeasts in garlic; Mucor spp., Penicillium spp., Monilia spp., Lactobacillus brevis and yeasts in both types of onions. Leuconostoc mesenteroides subsp. mesenteroides was detected in only kind of onion. In dehydrated garlic storage, Penicillium spp., Monilia spp., Lactobacillus brevis and yeasts were detected. In garlic, when a blanching step was carried out no microflora was detected. Mucor spp., Penicillium spp., Monilia spp. and Lactobacillus brevis were identified in both types of dehydrated onions. When brine immersion was included the microflora detected was significantly lower and only Penicillium spp. were found. The use of additional barriers such as blanching or brine immersion produces an important effect on the microbiological stability in these products.
Descritores: Bactérias/isolamento & purificação
Dessecação
Microbiologia de Alimentos
Conservação de Alimentos/métodos
Fungos/isolamento & purificação
Alho/microbiologia
Cebolas/microbiologia
Sais/farmacologia
-Candida/isolamento & purificação
Temperatura Alta
Lactobacillus/isolamento & purificação
Leuconostoc/isolamento & purificação
Mucor/isolamento & purificação
Penicillium/isolamento & purificação
Tipo de Publ: Estudo Comparativo
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-618849
Autor: Lillo, Luis; Muñoz, Inés; Pérez, Jerónimo; Alarcón, Julio; Céspedes, Carlos L; Cabello, gerardo; Lamilla, Claudio; Caro, Claudia.
Título: Solubility effects on antibacterial activity of chemically modified chitooligosaccharides of fungal origin / Efectos de la solubilidad sobre la actividad antibacteriana de quitooligosacáridos modificados químicamente de origen fúngico
Fonte: Bol. latinoam. Caribe plantas med. aromát;10(6):536-542, ene. 2011. ilus, tab.
Idioma: en.
Projeto: Universidad del Bío-Bío. Dirección de Investigación.
Resumo: Chitin and chitosan are a class of metabolites that occurring in some fungi species that are associated with commercial and medicinal plants, this is in Mucor sp. for example with an ample number of biological activities, being antibacterial and antifungal one of the most important. Into our program of search of biopesticides and natural compounds with biological activities, we have studying chitosan that was obtained from the culture medium of the fungus Mucor ruoxii. Chitooligosaccharides were prepared by partial acid hydrolysis of native chitosan and an aminoglycosylated derivative was obtained by reductive amination of the chitooligosaccaride. The solubilities of these compounds were measured at different pHs and its antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive). Chitosan and the derivatives tested exhibited a good antibacterial activity against S. aureus.

Quitina y quitosano son una clase de metabolitos que producen algunas especies de hongos que están asociados con plantas medicinales y comerciales, esto es por ejemplo en Mucor sp., con un amplio número de actividades biológicas, siendo la antibacteriana y antifúngica unas de las más importantes. En nuestro programa de investigación de biopesticidas y compuestos naturales, estamos estudiando quitosano obtenido de el medio de cultivo del hongo Mucor ruoxii. Quitooligosacáridos fueron preparados por hidrólisis parcial ácida de quitosano nativo y un derivado aminoglicosilado fue obtenido por aminación reductiva del quitooligosacárido. Las solubilidades de estos compuestos fueron medidas a diferentes pHs y su actividad antibacteriana frente a Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive). Quitosano y los derivados testeados exhiben una buena actividad antibacteriana frente a S. aureus.
Descritores: Antibacterianos/farmacologia
Escherichia coli
Mucor/química
Oligossacarídeos/farmacologia
Quitosana/farmacologia
Staphylococcus aureus
-Cromatografia em Gel
Concentração de Íons de Hidrogênio
Hidrólise
Solubilidade
Análise Espectral
Responsável: CL1.1 - Biblioteca Central


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Id: lil-530998
Autor: Martínez, J.
Título: Mucormicosis: infección necrotizante como complicación de dermolipectomía abdominal / Mucormycosis: necrotizing infection as a complication of abdominal dermolipectomy
Fonte: Col. med. estado Táchira;16(1):61-64, ene.-mar. 2007. ilus.
Idioma: es.
Resumo: Las infecciones necrotizantes de los tejidos blandos varían en su presentación clínica, pero en general son de curso grave y alta mortalidad. El cuadro clínico incluye: fiebre, celulitis, edema, crepitación, necrosis y sepsis; con frecuencia existe un antecedente o traumático quirúrgico. Como hallazgo operatorio se encuentra necrosis de la piel y tejido subcutáneo, con o sin mionecrosis. El tratamiento es una inmediata y amplia debridación acompañada con antibioticoterapia de amplio espectro. Se presenta un caso clínico de infección necrotizante de tejidos blandos por mucor, en una mujer con antecedente de abdominoplastia y lipoescultura con progresión crítica y evolución fatal. La mucormicosis es una patología agresiva, cuyo diagnostico clínico no es fácil por lo cual debe ser considerada seriamente por el cirujano plástico.
Descritores: Antibacterianos/uso terapêutico
Gangrena/patologia
Mucor/citologia
Mucor/isolamento & purificação
Mucormicose/cirurgia
Mucormicose/mortalidade
Mucormicose/patologia
Parede Abdominal/cirurgia
-Antibacterianos/farmacologia
Cesárea
Dermatomicoses/patologia
Dermatopatias Infecciosas/etiologia
Fungos/isolamento & purificação
Mucorales/isolamento & purificação
Limites: Humanos
Adulto
Feminino
Tipo de Publ: Relatos de Casos
Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Texto completo SciELO Chile
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Id: lil-524883
Autor: Setayesh, Ned A; Sepehrizadeh, Zargham; Jaberi, Elham; Yazdi, Mojtaba Tabatabaei.
Título: Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli
Fonte: Biol. Res;42(2):137-146, 2009. ilus, tab.
Idioma: en.
Projeto: Research Council of the Tehran University of Medical Sciences.
Resumo: The present work aims to study a new NADH-cytochrome b5 reductase (cb5r) from Mucor racemosus PTCC 5305. A cDNA coding for cb s r was isolated from a Mucor racemosus PTCC 5305 cDNA library. The nucleotide sequence of the cDNA including coding and sequences flanking regions was determined. The open reading frame starting from ATG and ending with TAG stop codon encoded 228 amino acids and displayed the closest similarity (73 percent) with Mortierella alpina cb s r. Lack of hydrophobic residues in the N-terminal sequence was apparent, suggesting that the enzyme is a soluble isoform. The coding sequence was then cloned in the pET16b transcription vector carrying an N-terminal-linked His-Tag® sequence and expressed in Escherichia coli BL21 (DE3). The enzyme was then homogeneously purified by a metal affinity column. The recombinant Mucor enzyme was shown to have its optimal activity at pH and temperature of about 7.5 and 40 °C, respectively. The apparent Km value was calculated to be 13 μM for ferricyanide. To our knowledge, this is the first report on cloning and expression of a native fungal soluble isoform of NADH-cytochrome b5 reductase in E. coli.
Descritores: /genética
CYTOCHROME-B(ABDOMEN) REDUCTASE/genética
DNA Complementar/genética
Escherichia coli/genética
Vetores Genéticos/genética
Mucor/enzimologia
-Sequência de Bases
Clonagem Molecular
/metabolismo
CYTOCHROME-B(ABDOMEN) REDUCTASE/metabolismo
Biblioteca Gênica
Isoenzimas/genética
Isoenzimas/metabolismo
Dados de Sequência Molecular
Fases de Leitura Aberta
Transcrição Genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde