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Couri, Sonia
Costa, Antonio Carlos Augusto da
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Id: biblio-1039128
Autor: Lima, Laisy Garcia Ribeiro; Gonçalves, Márcia Monteiro Machado; Couri, Sonia; Melo, Verônica Ferreira; Sant'Ana, Gizele Cardoso Fontes; Costa, Antônio Carlos Augusto da.
Título: Lipase Production by Aspergillus niger C by Submerged Fermentation
Fonte: Braz. arch. biol. technol;62:e19180113, 2019. tab, graf.
Idioma: en.
Resumo: Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1 yeast extract at pH 5.0) was the one that presented a lipase activity of 27.46 U.mL-1. It was very close to that best assay (30.76 U.mL-1), but using half of the inducer concentration, consequently reducing process cost. The kinetics of lipase production showed that the highest specific activity was 57.17 U.mg-1. The pH and temperature effects on lipase activity produced in this study was investigated. The optimum activity was found in a more acidic pH (5.0-6.0) and 55°C.
Descritores: Aspergillus niger/enzimologia
Lipase/análise
-Projetos de Pesquisa
Fermentação
Responsável: BR1.1 - BIREME


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Id: biblio-1055404
Autor: Lombardi, Julia; Ciocia, Felicia; Uniacke-Lowe, Thérèse; Boeris, Valeria; Risso, Patricia; McSweeney, Paul L. H.
Título: Application of an Enzymatic Extract from Aspergillus niger as Coagulant for Cheddar Cheese Manufacture
Fonte: Braz. arch. biol. technol;62:e19180128, 2019. tab, graf.
Idioma: en.
Projeto: Agencia Nacional de Promoción Científica y Tecnológica.
Resumo: Abstract The coagulation of milk by a serin protease from Aspergillus niger NRRL3 was studied by rheology. Cheddar-type cheese was manufactured using 3.5% (v/v) of fungal enzymatic extract and fermentation-produced chymosin was used as control coagulant. Full composition and ripening of both kinds of Cheddar cheese were studied. Differences in the proteolysis of caseins, not only during cheese manufacture but also during ripening, affected cheese composition, texture and peptide profile. Microbial development during ripening was not affected by the coagulant used.
Descritores: Aspergillus niger
Fenômenos Bioquímicos
Queijo
Agentes de Coagulação
Responsável: BR1.1 - BIREME


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Id: biblio-843148
Autor: Sepúlveda, Leonardo; de la Cruz, Reynaldo; Buenrostro, José Juan; Ascacio-Valdés, Juan Alberto; Aguilera-Carbó, Antonio Francisco; Prado, Arely; Rodríguez-Herrera, Raúl; Aguilar, Cristóbal Noé.
Título: Efecto de las diferentes fuentes de polifenoles sobre la eficiencia de liberación de ácido elágico por Aspergillus niger / Effect of different polyphenol sources on the efficiency of ellagic acid release by Aspergillus niger
Fonte: Rev. argent. microbiol;48(1):71-77, mar. 2016. graf, tab.
Idioma: en.
Resumo: Fungal hydrolysis of ellagitannins produces hexahydroxydiphenic acid, which is considered an intermediate molecule in ellagic acid release. Ellagic acid has important and desirable beneficial health properties. The aim of this work was to identify the effect of different sources of ellagitannins on the efficiency of ellagic acid release by Aspergillus niger. Three strains of A. niger (GH1, PSH and HT4) were assessed for ellagic acid release from different polyphenol sources: cranberry, creosote bush, and pomegranate used as substrate. Polyurethane foam was used as support for solid-state culture in column reactors. Ellagitannase activity was measured for each of the treatments. Ellagic acid was quantified by high performance liquid chromatography. When pomegranate polyphenols were used, a maximum value of ellagic acid (350.21 mg/g) was reached with A. niger HT4 in solid-state culture. The highest amount of ellagitannase (5176.81 U/l) was obtained at 8 h of culture when cranberry polyphenols and strain A. niger PSH were used. Results demonstrated the effect of different polyphenol sources and A. niger strains on ellagic acid release. It was observed that the best source for releasing ellagic acid was pomegranate polyphenols and A. niger HT4 strain, which has the ability to degrade these compounds for obtaining a potent bioactive molecule such as ellagic acid.

La hidrólisis fúngica de los elagitaninos produce ácido hexahidroxidifénico, considerado como una molécula intermedia en la liberación de ácido elágico. El ácido elágico tiene importantes y deseables propiedades benéficas para la salud humana. El objetivo de este trabajo fue identificar el efecto de la fuente de elagitaninos sobre la eficiente liberación de ácido elágico por Aspergillus niger. La liberación de ácido elágico se realizó con tres cepas de A. niger (GH1, PSH y HT4) en presencia de diferentes fuentes de polifenoles (arándano, gobernadora y granada), usadas como sustrato. Se empleó espuma de poliuretano como soporte para el cultivo en estado sólido en reactores en columna. Se midió la actividad elagitanasa a cada uno de los tratamientos. El ácido elágico liberado se cuantificó por cromatografía líquida de alta resolución. Cuando se utilizaron los polifenoles de granada, se alcanzó un valor máximo de 350,21 mg/g de ácido elágico con A. niger HT4 en cultivo en estado sólido. La mayor actividad elagitanasa (5176.81 U/l) se obtuvo a 8 h de cultivo cuando se usaron los polifenoles de arándano como sustrato y A. niger PSH. Los resultados demostraron el efecto que tiene la fuente de polifenoles y la cepa de A. niger en la liberación de ácido elágico. Se observó que la mejor fuente para la liberación de ácido elágico fueron los polifenoles de granada y que la cepa A. niger HT4 posee la habilidad de degradar estos compuestos para la obtención de potentes moléculas bioactivas, como el ácido elágico.
Descritores: Aspergillus niger/isolamento & purificação
Ácido Elágico/análise
Polifenóis/análise
-Aspergillus niger/fisiologia
Cromatografia Líquida de Alta Pressão/métodos
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1116352
Autor: Duo Filho, Valter Batista; Siqueira, João Paulo Zen; Colombo, Tatiana Elias.
Título: Monitoramento de fungos anemófilos no ambiente de uma biblioteca no Município de São José do Rio Preto - Sp, Brasil / Anemophilic fungi monitoring in a library environment in the city of São Jose do Rio Preto, Brazil
Fonte: Arq. ciências saúde UNIPAR;24(2):75-80, maio-ago. 2020.
Idioma: pt.
Resumo: Os fungos desempenham vários papéis que impactam a humanidade de diversas maneiras. Suas características metabólicas são importantes na biotecnologia, porém, tais microrganismos podem desencadear alguns problemas de saúde pública e até mesmo serem letais. Objetivo: detectar a presença de fungos no acervo de uma biblioteca no município de São José do Rio Preto. Metodologia: foram coletadas quarenta amostras nas superfícies inanimadas (livros, estantes, documentos, mapas, artigos e revistas) das principais salas da biblioteca com o auxílio de swabs umedecidos em solução salina estéril, posteriormente encaminhados ao laboratório de Biomedicina da Universidade Paulista ­ UNIP. As amostras foram semeadas em meio de cultura ágar Sabouraud Dextrose (SDA), tendo adicionado cloranfenicol e incubadas a 30 °C. Foi realizada a colônia gigante em todas as cepas crescidas em SDA para a realização da técnica de microcultivo para a identificação dos fungos, de acordo com o Manual de Detecção e Identificação dos Fungos de Importância Médica da Agência Nacional de Vigilância Sanitária. Resultados: Houve positividade em trinta e uma amostras (78%) e em quatro delas foi observado mais de um tipo de colônia (13%). Das vinte e duas superfícies de livros analisadas, foram isolados e identificados: Aspergillus flavus, Aspergillus niger, Cunninghamella sp., Cladosporium sp., Curvularia sp., Mucor sp. e Nigrospora sp. Nas oito superfícies de estantes: Aspergillus flavus, Aspergillus niger, Aspergillus versicolor, Penicillium sp. e Scopulariopsis sp. e, nos dez documentos: Aspergillus nidulans, Aspergillus sp., Cladosporium sp., Cunninghamella sp. e Trichoderma sp. Conclusão: Os fungos encontrados estão amplamente distribuídos no ambiente como solo e ar e, por diversos fatores, instalam-se em locais como bibliotecas. Em condições favoráveis, podem infectar o homem e causar perdas patrimoniais para os acervos.

Fungi play many roles that impact humankind in different ways. Their metabolic characteristics are important in biotechnology; however, these microorganisms can trigger some public health problems or may even be lethal. Objective: detect the presence of fungi in the collection of a public library in the city of São José do Rio Preto, Brazil. Methods: a total of forty samples were collected from inanimate surfaces (books, shelves, documents, maps, articles and magazines) located in the main rooms of the library with swabs soaked in sterile saline solution and sent to the Universidade Paulista ­ UNIP laboratories. The samples were plated in Sabouraud Dextrose Agar (SDA) supplemented with chloramphenicol and incubated at 30 °C. The colonies that grew in SDA were isolated in Potato Dextrose Agar for performing the slide culture technique for the identification of the fungi, performed according to the Manual of Detection and Identification of Fungi of Medical Importance from the Brazilian Health Surveillance Agency (ANVISA). Results: Thirty-one samples (78%) were positive, and in four of them more than one fungus genus was observed (13%). From the twenty-two book surfaces analyzed, the following fungi were isolated and identified: Aspergillus flavus, Aspergillus niger, Cunninghamella sp., Cladosporium sp., Curvularia sp., Mucor sp. and Nigrospora sp. On the eight shelves: Aspergillus flavus, Aspergillus niger, Aspergillus versicolor, Penicillium sp. and Scopulariopsis sp. The ten documents analyzed presented the following fungi: Aspergillus nidulans, Aspergillus sp., Cladosporium sp., Cunninghamella sp. and Trichoderma sp.. Conclusion: These fungi are widely distributed in the environment such as in the soil and air, and due to several factors, they colonize public places, such as libraries. In favorable conditions, they may infect humans and cause diseases.
Descritores: Monitoramento Ambiental
Acervo de Biblioteca
Fungos
-Penicillium
Aspergillus flavus
Aspergillus nidulans
Aspergillus niger
Trichoderma
Biotecnologia
Cladosporium
Cunninghamella
Ágar
Infecções
Responsável: BR513.1 - Biblioteca Central


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Id: biblio-950731
Autor: Mahilrajan, Subajini; Nandakumar, Jeyarani; Kailayalingam, Robika; Manoharan, Nilushiny Aloysius; SriVijeindran, SriThayalan.
Título: Screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts
Fonte: Biol. Res;47:1-5, 2014. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: The whitish tender leaves of Palmyrah are used for making handicrafts. The problem with these articles is discolouration with time and become more brittle due to fungal attack. This could be prevented by some protective coating. Instead of expensive and harmful chemicals we decided to test natural plant essential oils to control fungal attack. Palmyrah leaf article decay fungi were isolated from two different sites of Jaffna peninsula. In this investigation Antifungal Activity of different plant essential oils from neem (Azadirachta indica), castor (Ricinus communis), citronella (Cymbopogon sp) and camphor (Cinnamomum camphora) obtained from local market have been evaluated against isolated fungi. For screening of Antifungal activity, tests and controls were set to determine minimum inhibitory concentration (MIC) and Percentage of Growth Inhibition. RESULTS: Morphologically three different types of Palmyrah leaf decay fungi were isolated and characterized asAspergillus niger, Aspergillus flavus and Penicillium sp. Neem and castor oils have recorded no significant (0.05 > P) antifungal activity while citronella and camphor oils showed significantly different antifungal activity compared with control. Camphor oil and Citronella oil showed 100, 58.13% of average growth inhibition for A. niger. 96.38, 51.32% for A.flavus and 84.99, 72.76% forPenicillium sp respectively. Camphor oil showed highest percentage of growth inhibition at lowest minimum inhibitory concentration compared with citronella oil. Camphor oil was found to be highly antifungal and most effective against A niger, and A. flavus, compared with Penicillium sp and gave 100 percentage of growth inhibitions at 5, 1 and 15 ml/dl minimum inhibitory concentration respectively. CONCLUSION: Significantly higher broad-spectrum of antifungal activity was observed in camphor oil than other tested oils because it showed highest percentage of growth inhibition at lowest inhibitory concentration. Therefore it could be used for the development of new environmental friendly antifungal agent for the preservation of leafy handicrafts. Further formulation, field experiments are necessary to achieve this target.
Descritores: Penicillium/efeitos dos fármacos
Aspergillus/efeitos dos fármacos
Óleos Vegetais/farmacologia
Óleos Voláteis/farmacologia
Arecaceae/microbiologia
Inibidores do Crescimento/farmacologia
Antifúngicos/farmacologia
-Penicillium/isolamento & purificação
Penicillium/crescimento & desenvolvimento
Aspergillus/isolamento & purificação
Aspergillus/crescimento & desenvolvimento
Aspergillus flavus/isolamento & purificação
Aspergillus flavus/crescimento & desenvolvimento
Aspergillus flavus/efeitos dos fármacos
Aspergillus niger/isolamento & purificação
Aspergillus niger/crescimento & desenvolvimento
Aspergillus niger/efeitos dos fármacos
Ricinus/química
Testes de Sensibilidade Microbiana/métodos
Cinnamomum camphora/química
Azadirachta/química
Cymbopogon/química
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1041524
Autor: Santos, Rennan Luiz Oliveira dos; Domaneschi, Carina; Pedroso, Victhoria Beatriz; Florezi, Giovanna Piacenza; Ruiz, Luciana da Silva; Gandra, Rinaldo Ferreira; Sugaya, Norberto Nobuo; Paula, Claudete Rodrigues.
Título: Antifungal efficiency of chemically and thermally-activated acrylic resins after surface treatment using poly (diallyldimethylammonium chloride)
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190033, 2019. tab, graf.
Idioma: pt.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract INTRODUCTION: Acrylic resins are used in the preparation of facial prostheses and may be colonized by fungi. Here, we verified the antifungal efficacy of this material after surface treatment using poly (diallyldimethylammonium chloride). METHODS: Acrylic resin specimens with and without surface treatment were subjected to tests for fungistatic and fungicidal activities. Standard strains of Candida albicans and Aspergillus niger were used. RESULTS: After surface treatment, the fungistatic and fungicidal efficacies of the resins against C. albicans and fungistatic action against A. niger were verified. CONCLUSIONS: The surface treatment was a determinant of the antifungal activity of the material.
Descritores: Polietilenos/farmacologia
Aspergillus niger/efeitos dos fármacos
Temperatura
Resinas Acrílicas/química
Candida albicans/efeitos dos fármacos
Compostos de Amônio Quaternário/farmacologia
Antifúngicos/farmacologia
-Teste de Materiais
Testes de Sensibilidade Microbiana
Materiais Dentários
Responsável: BR1.1 - BIREME


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Id: biblio-1171783
Autor: Castrillo María L; Horianski Marta A; Jerke Gladis.
Título: Aislamiento de cepas de Aspergillus sección Nigri en la yerba mate comercializada en Posadas (Misiones, Argentina) y evaluación de su potencial ocratoxigénico / [Isolation of Aspergillus section Nigri strains in yerba mate in Posadas (Misiones, Argentina) and evaluation of their ochratoxigenic potential].
Fonte: Rev. argent. microbiol;45(2):110-3, jun. 2013.
Idioma: es.
Resumo: The objectives of the present work were to investigate the isolation frequency of genus Aspergillus in canchada yerba mate (YMCH) and elaborated yerba mate (YME) (Ilex paraguariensis) and the proportion of section Nigri isolates, as well as to determine ochratoxin A production by Aspergillus species section Nigri. Three hundred twenty eight Aspergillus strains from 20 samples of YMCH and 1306 Aspergillus strains from 36 samples of YME were isolated; of the total, 279 from the first group of strains and 1215 from the latter group, belonged to section Nigri. For the detection of ochratoxin A production, the strains were cultivated on Czapeck yeast extract agar and the toxin was detected by thin layer chromatography under UV light. Uniserate species predominance was observed in the 1494 strains of Aspergillus section Nigri obtained (Aspergillus japonicus var. japonicus and Aspergillus japonicus var. aculeatus), whereas none of the strains analysed showed ochratoxin A production in vitro at the detection level of the methodology employed.
Descritores: Aspergillus niger/isolamento & purificação
Aspergillus niger/metabolismo
Ilex paraguariensis/microbiologia
Ocratoxinas/biossíntese
-Argentina
Tipo de Publ: Resumo em Inglês
Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Texto completo SciELO Brasil
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Id: lil-789486
Autor: Chokoeva, Anastasiya Atanasova; Zisova, Liliya; Chorleva, Kristina; Tchernev, Georgi.
Título: Aspergillus niger – a possible new etiopathogenic agent in Tinea capitis? Presentation of two cases
Fonte: Braz. j. infect. dis;20(3):303-307, May.-June 2016. graf.
Idioma: en.
Resumo: Abstract Tinea capitis is generally considered as the most frequent fungal infection in childhood, as it accounts for approximately 92% of all mycosis in children. The epidemiology of this disease varies widely ranging from antropophillic, zoophilic, and geophillic dermatophytes, as the main causative agent in different geographic areas, depending on several additional factors. Nowadays, the etiology is considered to vary with age, as well with gender, and general health condition. The former reported extraordinary Tinea capitis case reports have been replaced by original articles and researches dealing with progressively changing patterns in etiology and clinical manifestation of the disease. This fact is indicative that under the umbrella of the well-known disease there are facts still hidden for future revelations. Herein, we present two rare cases of Tinea capitis in children, which totally differ from the recently established pattern, in their clinical presentation, as well as in the etiological aspect, as we discuss this potential new etiological pattern of the disease, focusing on our retrospective and clinical observation. Collected data suggest that pathogenic molds should be considered as a potential source of infection in some geographic regions, which require total rationalization of the former therapeutic conception, regarding the molds’ higher antimitotic resistance compared to dermatophytes. Molds-induced Tinea capitis should be also considered in clinically resistant and atypical cases, with further investigations of the antifungal susceptibility of the newest pathogens in the frame of the old disease. Further investigations are still needed to confirm or reject this proposal.
Descritores: Aspergilose/tratamento farmacológico
Aspergillus niger/isolamento & purificação
Tinha do Couro Cabeludo/microbiologia
-Couro Cabeludo/microbiologia
Tinha do Couro Cabeludo/tratamento farmacológico
Estudos Retrospectivos
Antifúngicos/uso terapêutico
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1013368
Autor: Dallagnol, Andrea Micaela; Bustos, Ana Yanina; Martos, Gladys Irma; Font de Valdez, Graciela; Gerez, Carla Luciana.
Título: Efecto antifúngico y antimicotoxigénico de Lactobacillus plantarum CRL 778 a diferentes valores de actividad de agua / Antifungal and antimycotoxigenic effect of Lactobacillus plantarum CRL 778 at different water activity values
Fonte: Rev. argent. microbiol;51(2):164-169, jun. 2019. ilus, tab.
Idioma: en.
Resumo: Ochratoxin A (OTA) is a mycotoxin produced by filamentous fungi with high impact Lactic acid bacteria; in food safety due to its toxicity. In the last decade, the presence of OTA was widely reported in different foods. In this study, the ability of Lactobacillus (L.) plantarum CRL 778 to control growth and OTA production by Aspergillus (A.) niger 13D strain, at different water activity (a w) values (0.955, 0.964, 0.971, 0.982, and 0.995) was determined in vitro. Both parame ters were significantly (p<0.05) reduced by the lactobacilli and the effect depended on a w. Greatest growth rate inhibition (46.9%) was obtained at a w = 0.995, which is the most suitable value for growth and production of antifungal metabolites (lactic acid, acetic acid, phenyllac-tic and hydroxyl-phenyllactic acids) by L. plantarum CRL 778. Besides, morphological changes and inhibition of melanin synthesis were observed in colonies of A. niger 13D in presence of L. plantarum CRL 778 at a w ranged between 0.971 and 0.995. In addition, maximum reduction (90%) of OTA production took place at a w = 0.971, while inhibition of fungi growth was more evident at a w =0.995. These findings suggest that L. plantarum CRL 778 could be used for control of ochratoxigenic fungal growth and OTA contamination in different fermented foods with a w values between 0.971 and 0.995.

Ocratoxina A (OTA) es una micotoxina producida por hongos filamentosos con un alto impacto en la seguridad alimentaria debido a su toxicidad. En la última década se ha reportado ampliamente a nivel mundial, la presencia de OTA en diversos alimentos. En este estudio se evaluó in vitro, la capacidad de Lactobacillus (L.) plantarum CRL 778 de controlar el crecimiento y la producción de OTA por Aspergillus (A.) niger 13D, a diferentes valores de actividad de agua (a w): 0.955, 0.964, 0.971,0.982 y 0.995). La cepa láctica redujo significativamente (p <0.05) ambos parámetros, siendo el efecto dependiente del valor de a w. La mayor inhibición del crecimiento (46.9%) se obtuvo a a w =0.995, valor más adecuado para el crecimiento y producción de metabolitos antifúngicos (ácido láctico, ácido acético, ácidos fenil-láctico e hidroxi-fenil láctico) por la cepa láctica. Además, se observaron cambios morfológicos en las colonias de A. niger 13D, crecidas en presencia de L. plantarum CRL 778 a valores de a w de 0.971 y 0.995. El porcentaje máximo de reducción en la producción de OTA (90%) por la cepa láctica se observó a un valor de a w = 0.971, mientras la inhibición del crecimiento fúngico fue mayor cuando a w = 0.995. Estos hallazgos sugieren que L. plantarum CRL 778 podría emplearse para el control de la contaminación por hongos ocratoxigénicos en alimentos con valores de aw comprendidos entre 0.971-0.995.
Descritores: Aspergillus niger/metabolismo
Lactobacillus plantarum/metabolismo
Antifúngicos/análise
-Aspergillus niger/crescimento & desenvolvimento
Contaminação de Alimentos/prevenção & controle
Ocratoxinas/antagonistas & inibidores
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: lil-477890
Autor: Casquero C, José; Guevara R, Miriam; Urcia A, Flor; Navarro M, Alida; Linares F, Nancy; Acurio U, Vilma; Huamaní B, Luis; Espinoza E, Nelva; Sotomayor E, Alfredto; Somocurcio V, José; Fernández V, Eduardo; Asmat M, Percy; Hurtado A, Elizabeth; Cornejo C, Marco.
Título: Frecuencia de aspergiloma en pacientes con antecedente de tuberculosis, hemoptisis, radiografía anormal y baciloscopía negativa / Frequency of aspergilloma in patients with history of tuberculosis, hemoptysis, abnormal chest x-ray films and negative sputum smears
Fonte: Rev. peru. med. exp. salud publica;23(2):104-109, abr.-jun. 2006. tab.
Idioma: es.
Resumo: Objetivo: Determinar la frecuencia de aspergilomas en pacientes con antecedentes de tuberculosis pulmonar curada,hemoptisis, radiografía de tórax anormal y BK negativo. Materiales y métodos: Se enroló 28 pacientes entre diciembrede 2002 y septiembre de 2004 en el Hospital Nacional Hipólito Unanue de Lima y Hospital Belén de Trujillo. A los pacientesevaluados en el Hospital Belén se les solicitó tres muestras de esputo, seriadas y consecutivas, mientras que lospacientes evaluados en el Hospital Nacional Hipólito Unanue y que fueron sometidos a cirugía de tórax, se les colectóuna porción de la pieza quirúrgica tisular pulmonar. Ambas muestras biológicas fueron cultivadas en agar sabourauddextrosa - cloramfenicol 0,05 por ciento e incubados a temperatura ambiente y 37 °C por 15 días. A todos los pacientes se lesextrajo 5 mL de sangre total para realizar la prueba de inmunodifusión contra Aspergillus sp. Resultados: La frecuenciade aspergiloma fue de 43 por ciento (12/28). Se identificó por cultivo Aspergillus fumigatus en cinco casos, Aspergillus sp endos, A. niger en uno, A. flavus en uno y en dos casos positivos por serología no se logró aislar el hongo. En los dospacientes donde se identificó A. niger, no se detectó presencia de anticuerpos contra Aspergillus sp. En el HospitalBelén la frecuencia de aspergiloma fue 39 por ciento (7/18) y en el Hospital Hipólito Unanue 50 por ciento (5/10). Conclusiones: Lafrecuencia de aspergiloma en los 28 pacientes investigados en ambos hospitales fue de 43 por ciento y el principal agenteetiológico fue A. fumigatus.

Objective: To determine the frequency of aspergilloma in patients with history of cured pulmonary tuberculosis, hemoptysis, abnormal chest X-ray films, and negative sputum smears for acid-fast bacilli. Materials and methods: 28 patients were enrolled between December 2002 and September 2004 in Hipolito Unanue National Hospital in Lima and Belen Hospital in Trujillo. Patients from Belen Hospital had three serial and consecutive sputum samples taken, while those patients from Hipolito Unanue Hospital who underwent thoracic surgery had a portion of the surgical specimen collected. Both biological samples were cultured in Sabouraud dextrose agar – 0,05% chloramphenicol, and they were incubated at room temperature and at 37° C for 15 days. All patients had a 5-mL blood sample taken in order to perform immunodifusion tests for Aspergillus sp. Results: The frequency of aspergilloma was 43%) (12/28). Aspergillus fumigatus was identified in cultures in five cases, Aspergillus sp. was identified in two cases, A. niger in one, A. flavus in one, and in two cases reported as positive for serological tests the fungus could not be isolated. In the two patients in whom A. niger was identified, no presence of antibodies against Aspergillus sp. was detected. In Belen Hospital the frequency of aspergilloma was 39% (7/18), and in Hipolito Unanue Hospital it was 50% (5/10). Conclusions: Aspergilloma frequency in 28 patients studied in both hospitals was 43%, and the main etiological agent was A. fumigatus.
Descritores: Aspergillus flavus
Aspergillus fumigatus
Aspergillus niger
Técnicas de Cultura
Responsável: PE14.1 - Biblioteca de la Sede Central



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