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Id: biblio-1026812
Autor: Sulieman, Ashraf K; Putra, Meilana Dharma; Abasaeed, Ahmed E; Gaily, Mohamed H; Al-Zahrani, Saeed M; Zeinelabdeen, Mohamed A.
Título: Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;34:1-8, july. 2018. tab, graf.
Idioma: en.
Resumo: Background: Ethanol and fructose are two important industrial products that enjoy many uses. In this contribution, their production via selective fermentation of date extract using Saccharomyces cerevisiae was studied. Scaling up the process for possible commercialization was investigated in three fermentors with working volume ratio of 1:40:400. Results: Higher ethanol concentration was obtained in the larger fermentor due to conversion of fructose. Fructose yields in the 0.5-L, 7.5-L and 80-L fermentors were 99, 92 and 90%, respectively. Good fitting was obtained with the modified Monod kinetics; however, a better fit of cell mass was obtained with the modified Ghose­Tyagi model which accounts for ethanol inhibition. Conclusions: The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.
Descritores: Saccharomyces cerevisiae/metabolismo
Etanol/síntese química
Frutose/biossíntese
-Leveduras
Cinética
Reatores Biológicos
Fermentação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1290554
Autor: Romero, Ana M; Snitman, Gabriela V; Marucco, Andrea P; Ponce, Graciela del V; Cataldi, Silvana P; Guelfand, Liliana I; Arechavala, Alicia.
Título: Evaluación del rendimiento del sistema Rapid™ Yeast Pluz (Remel) a partir de 2 medios de cultivo diferentes / Performance evaluation of Rapid™ Yeast Plus (Remel) system from two different culture media
Fonte: Rev. argent. microbiol;48(3):187-190, set. 2016. ilus.
Idioma: es.
Resumo: Dentro del género Candida, la especie más frecuentemente aislada de materiales clínicos es Candida albicans. Debido a la emergencia de otras especies que pueden presentar mayor índice de resistencia a los antifúngicos, se hace necesaria la identificación rápida de aquellas. El objetivo del presente trabajo fue evaluar el rendimiento del sistema RapID™ Yeast Plus a partir de subcultivos en 2 formulaciones diferentes, agar Sabouraud dextrosa modificado por Emmons (medio indicado en el inserto del equipo) y agar Sabouraud glucosado, que es el utilizado habitualmente en los laboratorios de la Ciudad Autónoma de Buenos Aires. Se estudiaron 166 cepas de muestras clínicas provenientes de los distintos hospitales que integran la Red de Micología de la Ciudad de Buenos Aires. De los resultados obtenidos se concluye que se deben mantener las condiciones y el medio de cultivo indicado por el fabricante

Within the genus Candida, Candida albicans is the most commonly isolated species from clinical samples. Due to the emergence of other species which can show a higher index of antifungal resistance, a fast identification of these species is necessary. The aim of this work was to evaluate the performance of the RapID Yeast Plus system from two different subculture media formulations: Sabouraud dextrose agar adjusted by Emmons (the medium is indicated in the equipment insert) and Sabouraud glucose agar, which is the most frequently used in Buenos Aires City laboratories. One hundred and sixty-six clinical sample strains coming from different hospitals belonging to the Mycology Network of Buenos Aires City were studied. From the obtained results, we conclude that the conditions and culture medium indicated by the manufacturer should be followed
Descritores: Leveduras/isolamento & purificação
Candida/classificação
Técnicas e Procedimentos Diagnósticos
Meios de Cultura/análise
-Candidíase/diagnóstico
Estudo de Avaliação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Estudo de Validação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1047827
Autor: Xu, Jian; Zhu, Yujing; Li, Hanchen; Chen, Limei; Chen, Wuxi; Cui, Min; Han, Lina; Hou, Wenbo; Li, Demao.
Título: Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
Fonte: Electron. j. biotechnol;35:10-17, sept. 2018. tab, graf.
Idioma: en.
Projeto: Hebei Province Science and Technology Support Program; . Key Program for International Science and Technology Cooperation Projects of China; . Hi-Tech Research and Development Program.
Resumo: Alanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface methodology as follows: 99.98-g/L glucose, 0.05-g/L yeast extract and a 183.17 dilution factor of the alanine mother liquid (v/v, with an alanine content of 0.72 g/L) and 17.98% inoculum concentration (v/v). Finally, in a 50-mL shake-flask fermentation, the DHA yield was 2.29 g/L.
Descritores: Ácidos Docosa-Hexaenoicos/biossíntese
Alanina/metabolismo
Estramenópilas/metabolismo
-Leveduras
Peptídeos e Proteínas de Sinalização Intercelular/isolamento & purificação
Alanina/análise
Fermentação
Glucose
Resíduos Industriais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1051261
Autor: Massoud, Ramona; Hadiani, Mohammad Rasoul; Hamzehlou, Pegah; Khosravi-Darani, Kianoush.
Título: Bioremediation of heavy metals in food industry: application of Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;37:56-60, Jan. 2019. tab.
Idioma: en.
Projeto: Shahid Beheshti University of Medical Sciences.
Resumo: Heavy metals are natural elements in the Earth's crust that can enter human food through industrial or agricultural processing, in the form of fertilizers and pesticides. These elements are not biodegradable. Some heavy metals are known as pollutants and are toxic, and their bioaccumulation in plant and animal tissues can cause undesirable effects for humans; therefore, their amount in water and food should always be under control. The aim of this study is to investigate the conditions for the bioremediation of heavy metals in foods. Various physical, chemical, and biological methods have been used to reduce the heavy metal content in the environment. During the last decades, bioremediation methods using plants and microorganisms have created interest to researchers for their advantages such as being more specific and environmentally friendly. The main pollutant elements in foods and beverages are lead, cadmium, arsenic, and mercury, which have their own permissible limits. Among the microorganisms that are capable of bioremediation of heavy metals, Saccharomyces cerevisiae is an interesting choice for its special characteristics and being safe for humans, which make it quite common and useful in the food industry. Its mass production as the byproduct of the fermentation industry and the low cost of culture media are the other advantages. The ability of this yeast to remove an individual separated element has also been widely investigated. In countries with high heavy metal pollution in wheat, the use of S. cerevisiae is a native solution for overcoming the problem of solution. This article summarizes the main conditions for heavy metal absorption by S. cerevisiae.
Descritores: Saccharomyces cerevisiae/metabolismo
Biodegradação Ambiental
Indústria Alimentícia
Metais Pesados/metabolismo
-Arsênio
Leveduras
Cádmio
Remoção de Contaminantes
Absorção
Bioacumulação
Chumbo
Mercúrio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1223215
Autor: Galdino Ribeiro, Beatriz; Oliveira de Veras, Bruno; Santos Aguiar, Jaciana dos; Campos Guerra, Jenyffer Medeiros; Asfora Sarubbo, Leonie.
Título: Biosurfactant produced by Candida utilis UFPEDA1009 with potential application in cookie formulation
Fonte: Electron. j. biotechnol;46:14-21, jul. 2020. tab, graf, ilus.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: BACKGROUND: Biosurfactants are biomolecules that have the potential to be applied in food formulations due to their low toxicity and ability to improve sensory parameters. Considering the ability of yeasts to produce biosurfactants with food-friendly properties, the aim of the present study was to apply a biosurfactant produced by Candida utilis in the formulation of cookies. RESULTS: The biosurfactant was obtained with a yield of 24.22 ± 0.23 g/L. The characterization analysis revealed that the structure of a metabolized fatty acid with high oleic acid content (68.63 ± 0.61%), and the thermogravimetric analysis demonstrated good stability at temperatures lower than 200°C, potential for food applications. The biosurfactant also exhibited satisfactory antioxidant activity at concentrations evaluated, without cytotoxic potential for cell strains, L929 and RAW 264.7, according to the (3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The incorporation of the surfactant into the dough of a standard cookie formulation to replace animal fat was carried out, achieving a softer, spongier product without significantly altering the physical and physicochemical properties or energy value. CONCLUSION: The thermal stability and antioxidant activity of the biosurfactant produced by C. utilis were verified, besides the positive contribution in the texture analysis of the cookies. Therefore, this biomolecule presents itself as a potential ingredient in flour-based sweet food formulations.
Descritores: Tensoativos/metabolismo
Candida/metabolismo
Alimentos Formulados
Biscoitos
-Temperatura
Leveduras
Indústria Alimentícia
Aditivos Alimentares
Antioxidantes
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-843145
Autor: Colabella, Fernando; Libkind, Diego.
Título: Un método basado en la PCR para la identificación rápida de levaduras acumuladoras de astaxantina (Phaffia spp. ) / PCR-based method for the rapid identification of astaxanthin-accumulating yeasts (Phaffia spp.)
Fonte: Rev. argent. microbiol;48(1):15-20, mar. 2016. ilus, graf, tab.
Idioma: en.
Resumo: It has been recently found that the natural distribution, habitat, and genetic diversity of astaxanthin-producing yeasts (i.e. Phaffia rhodozyma, synonym Xanthophyllomyces dendrorhous) is much greater than previously thought. P. rhodozyma is biotechnologically exploited due to its ability to produce the carotenoid pigment astaxanthin and thus, it is used as a natural source of this pigment for aquaculture. P. rhodozyma was also capable of synthesizing the potent UVB sunscreen mycosporine-glutaminol-glucoside (MGG). Therefore, further environmental studies are needed to elucidate its ecological aspects and detect new potential strains for the production of astaxanthin and MGG. However, obtaining new isolates of P. rhodozyma and related species is not always easy due to its low abundance and the presence of other sympatric and pigmented yeasts. In this work we report a successful development of a species-specific primer which has the ability to quickly and accurately detecting isolates representing all known lineages of the genus Phaffia (including novel species of the genus) and excluding closely related taxa. For this purpose, a primer of 20 nucleotides (called PhR) was designed to be used in combination with universal primers ITS3 and NL4 in a multiplex amplification. The proposed method has the sensitivity and specificity required for the precise detection of new isolates, and therefore represents an important tool for the environmental search for novel astaxanthin-producing yeasts.

Recientemente, se ha encontrado que la distribución natural, el hábitat y la diversidad genética de levaduras productoras de astaxantina (p. ej., Phaffia rhodozyma, sinónimo Xanthophyllomyces dendrorhous) son mucho mayores de lo que se pensaba. P. rhodozyma se explota biotecnológicamente debido a su capacidad para producir el pigmento carotenoide astaxantina y, por lo tanto, se utiliza como una fuente natural de este pigmento para la acuicultura. También se encontró que esta levadura es capaz de sintetizar el potente protector solar UVB micosporina-glutaminol-glucósido (MGG). Por lo tanto, más estudios ambientales para dilucidar sus aspectos ecológicos y detectar nuevas cepas potenciales productoras de astaxantina y MGG son necesarios. Sin embargo, la obtención de nuevos aislamientos de P. rhodozyma y especies relacionadas no siempre es fácil debido a su baja abundancia y a la presencia de otras levaduras simpátricas y pigmentadas. En este trabajo se describe el desarrollo exitoso de un cebador especie-específico que tiene la capacidad de detectar rápidamente y con precisión cepas representativas de todos los linajes del género Phaffia previamente reportados (incluyendo nuevas especies del género) y excluir especies estrechamente relacionadas. Para ello, se diseñó un cebador de 20 nucleótidos (denominado PhR) para ser utilizado en combinación con los cebadores universales ITS3 y NL4 en una amplificación multiplex. El método propuesto tiene la sensibilidad y la especificidad requerida para la detección precisa de nuevos aislamientos y, por lo tanto, representa una importante herramienta para la búsqueda ambiental de nuevas levaduras productoras de astaxantina.
Descritores: Leveduras/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
-Sensibilidade e Especificidade
Xantofilas/isolamento & purificação
Métodos
Nucleotídeos/análise
Tipo de Publ: Relatório Técnico
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1223246
Autor: Sriputorn, Benjaporn; Laopaiboon, Pattana; Phukoetphim, Niphaphat; Polsokchuak, Nilubol; Butkun, Khanittha; Laopaiboon, Lakkana.
Título: Enhancement of ethanol production efficiency in repeated-batch fermentation from sweet sorghum stem juice: effect of initial sugar, nitrogen and aeration
Fonte: Electron. j. biotechnol;46:55-64, jul. 2020. tab, graf.
Idioma: en.
Projeto: Ph.D. Program; . Post-doctoral Program.
Resumo: BACKGROUND: Ethanol concentration (PE), ethanol productivity (QP) and sugar consumption (SC) are important values in industrial ethanol production. In this study, initial sugar and nitrogen (urea) concentrations in sweet sorghum stem juice (SSJ) were optimized for high PE (≥10%, v/v), QP, (≥2.5 g/L·h) and SC (≥90%) by Saccharomyces cerevisiae SSJKKU01. Then, repeated-batch fermentations under normal gravity (NG) and high gravity (HG) conditions were studied. RESULTS: The initial sugar at 208 g/L and urea at 2.75 g/L were the optimum values to meet the criteria. At the initial yeast cell concentration of ~1 × 108 cells/mL, the PE, QP and SC were 97.06 g/L, 3.24 g/L·h and 95.43%, respectively. Repeated-batch fermentations showed that the ethanol production efficiency of eight successive cycles with and without aeration were not significantly different when the initial sugar of cycles 2 to 8 was under NG conditions (~140 g/L). Positive effects of aeration were observed when the initial sugar from cycle 2 was under HG conditions (180­200 g/L). The PE and QP under no aeration were consecutively lower from cycle 1 to cycle 6. Additionally, aeration affected ergosterol formation in yeast cell membrane at high ethanol concentrations, whereas trehalose content under all conditions was not different. CONCLUSION: Initial sugar, sufficient nitrogen and appropriated aeration are necessary for promoting yeast growth and ethanol fermentation. The SSJ was successfully used as an ethanol production medium for a high level of ethanol production. Aeration was not essential for repeated-batch fermentation under NG conditions, but it was beneficial under HG conditions.
Descritores: Saccharomyces cerevisiae/metabolismo
Sorghum/química
Etanol/metabolismo
-Saccharomyces cerevisiae/crescimento & desenvolvimento
Ureia
Leveduras/crescimento & desenvolvimento
Aeração
Sorghum/microbiologia
Etanol/análise
Açúcares
Sucos
Fermentação
Gravitação
Nitrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1095737
Autor: Veiga, Flávia Franco; Cézar, Joice dos Santos; Jarros, Isabele Carrilho; Negri, Melyssa.
Título: Fatores de virulência e susceptibilidade a antifúngicos de Cryptococcus Spp / Virulence factors and susceptibility to Cryptococcus Spp. antifungals
Fonte: Arq. ciências saúde UNIPAR;24(1):3-7, jan-abr. 2020.
Idioma: pt.
Resumo: Criptococose é uma doença grave que afeta tanto imunocomprometidos quanto imunocompetentes, com isso analisar a virulência é fundamental para novas terapêuticas. Objetivo: Analisar a capacidade de virulência e susceptibilidade aos antifúngicos de Cryptococcus spp. isolados de líquor de pacientes de hospital do norte do Paraná. Métodos: A partir de dois isolados clínicos C. neoformans e C. gattii, realizou-se a confirmação da identificação. Para a virulência, avaliou-se o tamanho da cápsula, capacidade de sobrevivência após exposição a neutrófilos, produção de melanina e urease. No antifungigrama por difusão em disco utilizou-se: anfotericina B, cetoconazol, voriconazol, itraconazol e miconazol. Resultados: C. gattii destaca-se por maior desenvolvimento da cápsula além da melhor capacidade de sobreviver a fagocitose em relação ao C. neoformans. No antifungigrama, ambos os isolados se apresentam sensíveis às drogas estudadas. Conclusão: Esses achados contribuem para a compreensão das diferentes patogêneses entre C. gattii e C. neoformans.

Cryptococcosis is a serious disease that can affect both immunocompromised and immunocompetent individuals, thus the virulence analysis is fundamental for the development of new treatments. Objective: To analyze the virulence and susceptibility of Cryptococcus spp. isolated from cerebrospinal fluid of patients from a hospital in the north of Paraná. Methods: From two clinical isolates, C. neoformans and C. gattii were confirmed and identified. For virulence, capsule size, survival capacity after exposure to neutrophils, melanin production and urease were evaluated. In the disc-diffusion method, the following antifungals were used: amphotericin B, ketoconazole, voriconazole, itraconazole and miconazole Results: It was observed that C. gattii presents greater results for development of the capsule beside presenting the best ability to survive phagocytosis in relation to C. neoformans. In the disc-diffusion method, both isolates presented sensitivity to the studied drugs. Conclusion: These findings contribute to the understanding of the different pathogens between C. gattii and C. neoformans.
Descritores: Criptococose/virologia
Fatores de Virulência/análise
Antifúngicos/análise
-Fagocitose
Urease/urina
Leveduras/virologia
Cápsulas/análise
Preparações Farmacêuticas
Anfotericina B/análise
Itraconazol
Cryptococcus neoformans/virologia
Ágar/análise
Cryptococcus gattii/virologia
Voriconazol
Melaninas/análise
Miconazol
Neutrófilos/virologia
Responsável: BR513.1 - Biblioteca Central


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Id: biblio-1247638
Autor: Cavalcante, João Pedro; Paulino, Gustavo Vasconcelos Bastos; Broetto, Leonardo; Morais, Thayná Melo de Lima; Ferreira, Sonia Maria Soares; Valente, Patricia; Landell, Melissa Fontes.
Título: Prevalence, antifungal susceptibility and virulence determinants of oral yeast species isolated from immunodeficient patients in Northeastern Brazil / Prevalência, suscetibilidade a antifúngicos e determinantes de virulência de leveduras orais isoladas de pacientes imunodeficientes do Nordeste do Brasil / Prevalencia, susceptibilidad antifúngica y determinantes de virulencia de levaduras orales aisladas de pacientes inmunodeficientes del Noreste de Brasil
Fonte: Rev. epidemiol. controle infecç;10(3):1-16, jul.-set. 2020. ilus.
Idioma: en.
Resumo: Justificativa e Objetivos: A candidíase oral tem uma ocorrência comum em pacientes imunocomprometidos. No entanto, outras infecções emergentes tornaram-se cada vez mais habituais. O objetivo deste estudo foi investigar a prevalência, os determinantes de virulência e a suscetibilidade a antifúngicos de leveduras que colonizam a mucosa de pacientes imunocomprometidos na região Nordeste do Brasil. Métodos: A amostra foi composta por 60 pacientes HIV positivos atendidos no Serviço de Atendimento Especializado/Hospital Dia do Hospital Universitário Prof. Alberto Antunes, vinculado à Universidade Federal de Alagoas. As amostras foram coletadas em regiões subgengivais e semeadas em CHROMagar para confirmação presuntiva de Candida spp., seguido por PCR e sequenciamento. Além disso, testamos os determinantes de virulência fosfolipase e protease e avaliamos in vitro a concentração inibitória mínima dos antifúngicos anfotericina B e fluconazol. Este projeto foi aprovado pelo Comitê de ética em pesquisa do Centro de Estudos Superiores de Maceió. Resultados: Aproximadamente 63% dos pacientes foram colonizados por leveduras. A espécie C. albicans foi predominante, enquanto as espécies de Candida não-albicans representaram 49% dos isolados, sendo C. dubliniensis e C. parapsilosis as mais comuns. Entretanto, C. intermedia, Bullera penniseticola e Naganishia liquefaciens também foram encontrados. Os determinantes da virulência protease e/ou fosfolipase também foram produzidos por Candida spp. e alguns isolados oportunistas incomuns como Kodamaea ohmeri, N. liquefaciens e Saitozyma podzolica. Além disso, a maioria dos isolados de Candida spp. e algumas espécies oportunistas incomuns apresentaram altos valores de concentração inibitória mínima. Conclusão: Os resultados obtidos indicam que C. albicans continua a ser a espécie predominante na cavidade oral de pacientes imunodeficientes e, juntamente com outras espécies incomuns, pode apresentar alta resistência aos antifúngicos testados.(AU)

Background and Objectives: Oral candidiasis has a common occurrence in immunocompromised patients. However, other emergent infections have become increasingly common. The aim of this study was to investigate the prevalence, virulence determinants and the antifungal susceptibility of yeast colonizing the mucosa of immunocompromised patients in Northeastern Brazil. Methods: Samples from sixty HIV-positive patients seen at the Specialized Service / Hospital Dia - Hospital Universitário Prof. Alberto Antunes from the Federal University of Alagoas were collected from subgingival sites and seeded on CHROMagar for presumptive confirmation of Candida spp. followed by PCR and sequencing. In addition, we tested virulence determinants, phospholipase and protease and evaluated in vitro the Minimum Inhibitory Concentration of antifungals amphotericin B and fluconazole. This project was approved by the Research Ethics Committee of the Center for Higher Studies in Maceió. Results: Approximately 63% of the patients were colonized by yeasts, with C. albicans as the predominant species, while non-Candida albicans species accounted for 49% of the isolates, with C. dubliniensis and C. parapsilosis being the commonest, but C. intermedia, Bullera penniseticola and Naganishia liquefaciens were also found. The virulence determinants protease and/or phospholipase were also produced by Candida spp. and some uncommon opportunistic isolates such as Kodamaea ohmeri, N. liquefaciens and Saitozyma podzolica. Furthermore, most of Candida spp. strains and some uncommon opportunistic species showed high values of minimal inhibitory concentration. Conclusion: Results obtained indicate that C. albicans continues to be the predominant species in oral cavity of immunodeficient patients and along with other unusual species may present high resistance to the antifungals tested.(AU)

Justificación y Objetivos: La candidiasis oral acomete con frecuencia a pacientes inmunocomprometidos. Sin embargo, otras infecciones emergentes se han vuelto cada vez más comunes. El objetivo de este estudio fue investigar la prevalencia, la producción de determinantes de virulencia y la susceptibilidad a antifúngicos de levaduras que colonizan la mucosa de pacientes inmunocomprometidos en la región Nordeste de Brasil. Métodos: Se colectaron muestras de sesenta pacientes VIH positivos atendidos en el Servicio de Atención Especializado/Hospital Día del Hospital Universitario Prof. Alberto Antunes, vinculado a la Universidad Federal de Alagoas. Se colectaron las muestras en las regiones subgingivales y las sembraron en CHROMagar para la presunta confirmación de Candida spp. seguido de PCR y secuenciación. Además, analizamos los determinantes de virulencia fosfolipasa y proteasa y evaluamos in vitro la concentración mínima inhibitoria de los antifúngicos anfotericina B y fluconazol. Este proyecto fue aprobado por el Comité de Ética en Investigación del Centro de Estudios Superiores de Maceió. Resultados: Aproximadamente el 63% de los pacientes fueron colonizados por levaduras, y la C. albicans fue la especie predominante, mientras que las especies de Candida no-albicans representaron el 49% de los aislamientos, de las cuales la C. dubliniensis y la C. parapsilosis fueron las más comunes. Sin embargo, también se encontraron C. intermedia, Bullera penniseticola y Naganishia liquefaciens. Los determinantes de virulencia de proteasa y/o fosfolipasa también fueron producidos por Candida spp. y algunos aislados oportunistas inusuales como Kodamaea ohmeri, N. liquefaciens y Saitozyma podzolica. Además, la mayoría de los asilados de Candida spp. y algunas especies oportunistas inusuales mostraron valores altos de concentración mínima inhibitoria. Conclusión: Los resultados obtenidos indican que C. albicans continúa siendo la especie predominante en la cavidad oral de pacientes inmunodeprimidos y, junto con otras especies poco comunes, puede presentar una alta resistencia a los antifúngicos evaluados.(AU)
Descritores: Virulência
Leveduras/virologia
Candida
Candidíase Bucal
Fatores de Virulência
Síndromes de Imunodeficiência
Antifúngicos
-Prevalência
Síndrome de Imunodeficiência Adquirida
Limites: Humanos
Responsável: BR1314.1 - Biblioteca Central


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Id: biblio-1058113
Autor: Bonifaz, Alexandro; Montelongo-Martínez, Fernando; Araiza, Javier; González, Gloria María; Treviño-Rangel, Rogelio; Flores-Garduño, Alejandro; Camacho-Cruz, Alejandro; Tirado-Sánchez, Andrés.
Título: Evaluación de MALDI-TOF MS para la identificación de levaduras patógenas oportunistas de muestras clínicas / Evaluation of MALDI-TOF MS for the identification of opportunistic pathogenic yeasts of clinical samples
Fonte: Rev. chil. infectol;36(6):790-793, dic. 2019. tab.
Idioma: es.
Resumo: Resumen La espectrometría de masas MALDI-TOF MS es una técnica rápida y sencilla para identificar microorganismos por análisis proteico. Se estudiaron 304 aislados de levaduras procedentes de micosis superficiales y profundas, con el objetivo de comparar tres métodos: convencional (bioquímico y morfológico), MALDI-TOF MS, y reacción en cadena de la polimerasa (RPC, método de referencia). Se estudiaron 24 especies con predominio de Candida spp y Cryptococcus spp. La identificación por método convencional fue de 258/304 cepas, mientras que por MALDI-TOF MS fue de: 277/304 cepas (84,8 versus 91,2%, p = no significativo). El coeficiente Kappa entre el MALDI-TOF MS y la RPC reportó una excelente concordancia (0,99). La sensibilidad y la especificidad de MALDI-TOF MS para la identificación de levaduras patógenas oportunistas de muestras clínicas fueron de 94,6% y 99%; respectivamente. MALDI-TOF MS demostró ser una herramienta de alta precisión para la identificación de levaduras patógenas.

MALDI-TOF MS mass spectrometry is a rapid and straightforward technique to identify microorganisms by protein analysis. The study was performed in 304 yeast isolates from superficial and deep mycoses, in order to compare three methods: conventional (biochemical and morphological), MALDI-TOF MS, and polymerase chain reaction (PCR, reference). We included 24 species with predominance of Candida spp and Cryptococcus spp. The identification by conventional methods was 258/304 strains, while by MALDI-TOF MS was: 277/304 strains (84.8% versus 91.2%, P = not significant). The Kappa coefficient comparing MALDI-TOF-MS with PCR reported excellent concordance (0.99). The sensitivity and specificity of MALDI-TOF MS for the diagnosis of opportunistic pathogenic yeasts of clinical samples were 94.6% and 99% respectively. MALDI-TOF MS is a simple, fast and reliable tool for pathogenic yeasts.
Descritores: Micoses
-Leveduras
Candida/genética
Sensibilidade e Especificidade
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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