||Shi, Xiaodong; Wu, Yan; Dai, Tingwei; Gu, Yuxi; Wang, Linghui; Qin, Xiaobo; Xu, Ying; Chen, Fang.|
||JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco|
||Electron. j. biotechnol;34:76-82, july. 2018. ilus, graf.
||National Key Technology R&D Program of 12th Five-Year Plan of China; . Open Foundation of Key Laboratory of Molecular Genetics, China National Tobacco Corporation (Guizhou Academy of Tobacco Science); . Special Project for Breeding and Cultivation of GMO Varieties of Ministry of Agriculture.
||Background: Jatropha curcas L., as an important strategic biofuel resource with considerable economic potential, has attracted worldwide attention. However, J. curcas has yet to be domesticated. Plant height, an important agronomic trait of J. curcas, has not been sufficiently improved, and the genetic regulation of this trait in J. curcas is not fully understood. Zinc finger proteins (ZFPs), a class of transcription factors, have previously been shown to play critical roles in regulating multiple aspects of plant growth and development and may accordingly be implicated in the genetic regulation of plant height in J. curcas. Results: In this study, we cloned JcZFP8, a C2H2 ZFP gene in J. curcas. We found that the JcZFP8 protein was localized in the nucleus and contained a conserved QALGGH motif in its C2H2 structure. Furthermore, ectopic expression of JcZFP8 under the control of the 35S promoter in transgenic tobacco resulted in dwarf plants with malformed leaves. However, when JcZFP8 was knocked out, the transgenic tobacco did not show the dwarf phenotype. After treatment with the gibberellic acid (GA) biosynthesis inhibitor paclobutrazol (PAC), the dwarf phenotype was more severe than plants that did not receive the PAC treatment, whereas application of exogenous gibberellin3 (GA3) reduced the dwarf phenotype in transgenic plants. Conclusions: The results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant. How to cite: Shi X,Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco.|
Dedos de Zinco CYS2-HIS2/genética
||-Reguladores de Crescimento de Plantas/genética|
Fatores de Transcrição
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
||CL1.1 - Biblioteca Central|