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Id: biblio-1047373
Autor: Shi, Xiaodong; Wu, Yan; Dai, Tingwei; Gu, Yuxi; Wang, Linghui; Qin, Xiaobo; Xu, Ying; Chen, Fang.
Título: JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
Fonte: Electron. j. biotechnol;34:76-82, july. 2018. ilus, graf.
Idioma: en.
Projeto: National Key Technology R&D Program of 12th Five-Year Plan of China; . Open Foundation of Key Laboratory of Molecular Genetics, China National Tobacco Corporation (Guizhou Academy of Tobacco Science); . Special Project for Breeding and Cultivation of GMO Varieties of Ministry of Agriculture.
Resumo: Background: Jatropha curcas L., as an important strategic biofuel resource with considerable economic potential, has attracted worldwide attention. However, J. curcas has yet to be domesticated. Plant height, an important agronomic trait of J. curcas, has not been sufficiently improved, and the genetic regulation of this trait in J. curcas is not fully understood. Zinc finger proteins (ZFPs), a class of transcription factors, have previously been shown to play critical roles in regulating multiple aspects of plant growth and development and may accordingly be implicated in the genetic regulation of plant height in J. curcas. Results: In this study, we cloned JcZFP8, a C2H2 ZFP gene in J. curcas. We found that the JcZFP8 protein was localized in the nucleus and contained a conserved QALGGH motif in its C2H2 structure. Furthermore, ectopic expression of JcZFP8 under the control of the 35S promoter in transgenic tobacco resulted in dwarf plants with malformed leaves. However, when JcZFP8 was knocked out, the transgenic tobacco did not show the dwarf phenotype. After treatment with the gibberellic acid (GA) biosynthesis inhibitor paclobutrazol (PAC), the dwarf phenotype was more severe than plants that did not receive the PAC treatment, whereas application of exogenous gibberellin3 (GA3) reduced the dwarf phenotype in transgenic plants. Conclusions: The results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant. How to cite: Shi X,Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco.
Descritores: Tabaco/genética
Jatropha
Desenvolvimento Vegetal
Dedos de Zinco CYS2-HIS2/genética
-Reguladores de Crescimento de Plantas/genética
Fatores de Transcrição
Triazóis
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Clonagem Molecular
Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Giberelinas
Responsável: CL1.1 - Biblioteca Central


  2 / 128 LILACS  
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Id: biblio-1048187
Autor: Jiménez-Guillen, Doribet; Pérez-Pascual, Daniel; Souza-Perera, Ramón; Godoy-Hernández, Gregorio; Zúñiga-Aguilar, José Juan.
Título: Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition
Fonte: Electron. j. biotechnol;36:34-46, nov. 2018. tab, ilus.
Idioma: en.
Projeto: Consejo Nacional de Ciencia y Tecnología; . Comisión Intersecretarial de Bioseguridad de los Organismos Genéticamente Modificados; . DJG and DPP received CONACYT Ph.D..
Resumo: Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of somatic embryogenesis can be useful to understand the molecular regulation of the cell-to embryo transition, and these promoter elements represent biotechnological tools in plant organ tissue culture. Results: A −1,620 bp DNA sequence located upstream of the Coffea canephora SERK1 gene homologue (CcSERK1) was isolated, and then, different segments containing key response elements (REs) for somatic embryogenesis onset and development were fused to the uidA (encoding a ß-glucuronidase, GUS) reporter gene to evaluate its expression in transgenic leaf explants. DNA segments of −1,620 and −1048 bp in length directed uidA expression with patterns in leaf explants similar to those occurring during somatic embryogenesis. When a −792-bp fragment was used, uidA expression disappeared only in leaf explants and pro-embryogenic mass but persisted in developing embryos. No uidA expression was detected in any embryogenic stage when a −618-bp fragment was used. Conclusion: DNA deletions showed that a −1048-bp sequence located upstream of the CcSERK1 gene is sufficient to direct gene expression during the onset and the development of C. canephora somatic embryogenesis. The DNA segment located between −1048 and −792 bp (containing BBM and WUS REs) is needed for gene expression before embryogenesis onset but not during embryo development. The promoter segment between −792 and −618 bp (including GATA, ARR1AT, and ANT REs) regulates gene expression in developing embryos.
Descritores: Proteínas de Plantas/genética
Proteínas Quinases/genética
Coffea/genética
-Biotecnologia
Expressão Gênica
Regiões Promotoras Genéticas
Plantas Geneticamente Modificadas
Clonagem Molecular
Genes Reporter
Regulação da Expressão Gênica de Plantas
Desenvolvimento Embrionário
Responsável: CL1.1 - Biblioteca Central


  3 / 128 LILACS  
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Id: biblio-1047978
Autor: Li, Jun; Zhao, Aichun; Yu, Maode; Li, Yaofeng; Liu, Xiaoqing; Chen, Xiangyun.
Título: Function analysis of anthocyanidin synthase from Morus alba L. by expression in bacteria and tobacco
Fonte: Electron. j. biotechnol;36:9-14, nov. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Science and Technology Department of Guizhou Province; . Fund of Guizhou for Construction of First-class Discipline in China; . Specialized Fund for the Top Talent of Science and Technology in colleges and universities of Guizhou; . Scientific and Technological Projects of Guizhou Province.
Resumo: Background: Flavonoids are a kind of important secondary metabolite and are commonly considered to provide protection to plants against stress and UV-B for a long time. Anthocyanidin synthase (ANS), which encodes a dioxygenase in the flavonoid pathway, catalyzes the conversion of leucoanthocyanidins to anthocyanidins, but there is no direct evidence indicating that it provides tolerance to stress in plants. Results: To investigate whether ANS can increase tolerance to abiotic stress, MaANS was isolated from mulberry fruits and transformed into tobacco. Our results suggested that the bacterially expressed MaANS protein can convert dihydroquercetin to quercetin. Overexpression of MaANS remarkably increased the accumulation of total flavonoids in transgenic lines and anthocyanins in corollas of flowers. Transgenic lines showed higher tolerance to NaCl and mannitol stress. Conclusions: These results indicated that MaANS participates in various dioxygenase activities, and it can protect plants against abiotic stress by improving the ROS-scavenging ability. Thus, this alternative approach in crop breeding can be considered in the improvement of stress tolerance by enriching flavonoid production in plants
Descritores: Oxigenases/metabolismo
Tabaco
Morus/enzimologia
-Oxigenases/genética
Quercetina
Estresse Fisiológico
Bactérias
Flavonoides/metabolismo
Plantas Geneticamente Modificadas
Dioxigenases/metabolismo
Expressão Ectópica do Gene
Responsável: CL1.1 - Biblioteca Central


  4 / 128 LILACS  
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Id: biblio-1253093
Autor: Umer, Noroza; Zahra Naqvi, Rubab; Rauf, Imran; Anjum, Naveed; Keen, Patricia R; Van Eck, Joyce; Jander, Georg; Asif, Muhammad.
Título: Expression of Pinellia ternata leaf agglutinin under rolC promoter confers resistance against a phytophagous sap sucking aphid, Myzus persicae
Fonte: Electron. j. biotechnol;47:72-82, sept. 2020. tab, ilus, graf.
Idioma: en.
Projeto: International Research Support Initiative Program of Higher Education Commission (HEC) of; . Pakistan; . United States Department of Agriculture (USDA).
Resumo: BACKGROUND: Piercing/sucking insect pests in the order Hemiptera causes substantial crop losses by removing photoassimilates and transmitting viruses to their host plants. Cloning and heterologous expression of plantderived insect resistance genes is a promising approach to control aphids and other sap-sucking insect pests. While expression from the constitutive 35S promoter provides broad protection, the phloem-specific rolC promoter provides better defense against sap sucking insects. The selection of plant-derived insect resistance genes for expression in crop species will minimize bio-safety concerns. RESULTS: Pinellia ternata leaf agglutinin gene (pta), encodes an insecticidal lectin, was isolated and cloned under the 35S and rolC promoters in the pGA482 plant transformation vector for Agrobacterium-mediated tobacco transformation. Integration and expression of the transgene was validated by Southern blotting and qRT-PCR, respectively. Insect bioassays data of transgenic tobacco plants showed that expression of pta under rolC promoter caused 100% aphid mortality and reduced aphid fecundity up to 70% in transgenic tobacco line LRP9. These results highlight the better effectivity of pta under rolC promoter to control phloem feeders, aphids. CONCLUSIONS: These findings suggested the potential of PTA against aphids and other sap sucking insect pests. Evaluation of gene in tobacco under two different promoters; 35S constitutive promoter and rolC phloemspecific promoter could be successfully use for other crop plants particularly in cotton. Development of transgenic cotton plants using plant-derived insecticidal, PTA, would be key step towards commercialization of environmentally safe insect-resistant crops.
Descritores: Afídeos/patogenicidade
Controle Biológico de Vetores
Pinellia/química
-Vírus de Plantas
Tabaco
Southern Blotting
Reação em Cadeia da Polimerase
Regiões Promotoras Genéticas
Plantas Geneticamente Modificadas
Folhas de Planta/química
Transgenes
Resistência à Doença
Proteção de Cultivos
Responsável: CL1.1 - Biblioteca Central


  5 / 128 LILACS  
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Id: biblio-1177370
Autor: Arévalo Gallegos, Sigifredo; Varela Rodríguez, Hugo; Lugo Aguilar, Héctor; Siqueiros Cendón, Tania S; Iglesias Figueroa, Blanca F; Espinoza Sánchez, Edward A; Aguado Santacruz, Gerardo A; Rascón Cruz, Quintín.
Título: Transient expression of a green fluorescent protein in tobacco and maize chloroplast
Fonte: Electron. j. biotechnol;45:1-9, May 15, 2020. ilus.
Idioma: en.
Resumo: BACKGROUND: Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant condition of the crop. RESULTS: In this study, we constructed two different vectors with homologous recombination sequences from maize (Zea mays var. LPC13) and grass (Bouteloua gracilis var. ex Steud) (pZmcpGFP and pBgcpGFP, respectively). Both vectors were designed to integrate into rrn23S/rrn16S from an inverted repeat region in the chloroplast genome. Moreover, the vector had the mgfp5 gene driven by Prrn, a leader sequence of the atpB gene and a terminator sequence from the rbcL gene. Also, constructs have an hph gene as a selection marker gene driven by Prrn, a leader sequence from rbcL gene and a terminator sequence from the rbcL gene. Explants of maize, tobacco and Escherichia coli cells were transformed with both vectors to evaluate the transitory expression­an exhibition of green and red fluorescent light under epifluorescence microscopy. These results showed that both vectors were expressed; the reporter gene in all three organisms confirmed the capacity of the vectors to express genes in the cell compartments. CONCLUSIONS: This paper is the first report of transient expression of GFP in maize embryos and offers new information for genetically improving recalcitrant crops; it also opens new possibilities for the improvement in maize chloroplast transformation with these vectors.
Descritores: Tabaco/metabolismo
Cloroplastos/genética
Cloroplastos/metabolismo
Zea mays/genética
Proteínas de Fluorescência Verde/metabolismo
-Transformação Genética
Biotecnologia
Reação em Cadeia da Polimerase
Plantas Geneticamente Modificadas
Plastídeos/genética
Proteínas de Fluorescência Verde/genética
Escherichia coli
Genoma de Cloroplastos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Texto completo
Id: biblio-1278451
Autor: Liu, Feng; Ruan, Yingying.
Título: Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
Fonte: Braz. arch. biol. technol;64:e21200316, 2021. tab, graf.
Idioma: en.
Projeto: Ningbo City College of Vocational Technology.
Resumo: Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
Descritores: Plantas Geneticamente Modificadas
Arabidopsis
Fragaria
Herbicidas/efeitos adversos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-700397
Autor: Meneses, Claudio; Orellana, Ariel.
Título: Using genomics to improve fruit quality
Fonte: Biol. Res;46(4):347-352, 2013. tab.
Idioma: en.
Resumo: New fruit varieties are needed to satisfy consumers, and the industry is facing new challenges in order to respond to these demands. The emergence of genomic tools is releasing information on polymorphisms that can be utilized to expedite breeding processes in species that are difficult to breed, given the long periods of time required to get new varieties. The present review describes the current stages of the ongoing efforts that are being taken to apply these technologies to obtain varieties with improved fruit quality in species of the family Rosaceae.
Descritores: Plantas Geneticamente Modificadas
Rosaceae/genética
Frutas/genética
-Biotecnologia/métodos
Cruzamento/métodos
Genômica
Rosaceae/classificação
Rosaceae/crescimento & desenvolvimento
Frutas/crescimento & desenvolvimento
Valor Nutritivo
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
Texto completo
Id: biblio-950778
Autor: Muzaffar, Adnan; Kiani, Sarfraz; Khan, Muhammad Azmat Ullah; Rao, Abdul Qayyum; Ali, Arfan; Awan, Mudassar Fareed; Iqbal, Adnan; Nasir, Idrees Ahmad; Shahid, Ahmad Ali; Husnain, Tayyab.
Título: Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Fonte: Biol. Res;48:1-11, 2015. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed that Cry1Ac and Cry2A were successfully cloned in the correct orientation. The appearance of a blue color in sections of infiltrated leaves after 72 hours confirmed the successful expression of the construct in the plant expression system. The overall transformation efficiency was calculated to be 0.7%. The amplification of Cry1Ac-Cry2A and Tp2 showed the successful integration of target genes into the genome of cotton plants. A maximum of 0.673 µg/g tissue of Cry1Ac and 0.568 µg/g tissue of Cry2A was observed in transgenic plants. We obtained 100% mortality in the target insect after 72 hours of feeding the 2nd instar larvae with transgenic plants. The appearance of a yellow color in transgenic cross sections, while absent in the control, through phase contrast microscopy indicated chloroplast localization of the target protein. CONCLUSION: Locating the target protein at the point of insect attack increases insect mortality when compared with that of other transgenic plants. The results of this study will also be of great value from a biosafety point of view.
Descritores: Proteínas de Bactérias/genética
Proteínas Recombinantes de Fusão
Cloroplastos/genética
Controle de Insetos/métodos
Gossypium/genética
Endotoxinas/genética
Proteínas Hemolisinas/genética
Lepidópteros
-Bacillus thuringiensis
Proteínas de Bactérias/análise
Resistência a Inseticidas/genética
Imuno-Histoquímica
Expressão Gênica/genética
Cloroplastos/metabolismo
Reação em Cadeia da Polimerase
Microscopia de Contraste de Fase
Plantas Geneticamente Modificadas
Clonagem Molecular
Primers do DNA
Folhas de Planta/genética
Transgenes/fisiologia
Endotoxinas/análise
Fusão Gênica
Proteínas Hemolisinas/análise
Inseticidas
Larva
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 128 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1132198
Autor: Jadhav, Murlidhar Shrihari; Rathnasamy, Sakthi Ambothi; Natarajan, Balakrishnan; Duraialagaraja, Sudhakar; Varatharajalu, Udayasuriyan.
Título: Study of Expression of Indigenous Bt cry2AX1 Gene in T3 Progeny of Cotton and its Efficacy Against Helicoverpa armigera (Hubner)
Fonte: Braz. arch. biol. technol;63:e20180428, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Development of transgenic Bt crops with stable and high level of Bt protein expression over generations under different environmental conditions is critical for successful deployment at field level. In the present study, progenies of transgenic cotton Coker310 event, CH12 expressing novel cry2AX1 gene were evaluated in T3 generation for stable integration, expression and resistance against cotton bollworm, Helicoverpa armigera. The cry2AX1 gene showed stable inheritance and integration in the T3 progeny plants as revealed by PCR and Southern blot hybridization. The expression of Cry2AX1 protein on 90 days after sowing (DAS) was in the range of 1.055 to 1.5 µg/g of fresh leaf tissue except one plant which showed 0.806 µg/g of fresh leaf tissue and after 30 days (i.e., on 120 DAS) three plants recorded in between 0.69 to 0.82 µg/g and other plants are in range of 0.918 to 1.058 µg/g of fresh leaf tissue. Detached leaf bit bioassay in T3 progeny on 110 DAS recorded mortality of 73.33 to 93.33 per cent against H. armigera and severe growth retardation in surviving larvae. These results indicate that the expression of chimeric cry2AX1 is stable and exhibits insecticidal activity against H. armigera in T3 progeny of CH12 event of transgenic cotton.
Descritores: Bacillus thuringiensis/patogenicidade
Controle Biológico de Vetores/métodos
Gossypium/genética
Endotoxinas/genética
Mariposas
-Doenças das Plantas/prevenção & controle
Plantas Tóxicas
Bioensaio
Plantas Geneticamente Modificadas
Limites: Animais
Responsável: BR1.1 - BIREME


  10 / 128 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: biblio-950857
Autor: Liu, Yu; Wang, Lu; Liu, Heng; Zhao, Rongrong; Liu, Bin; Fu, Quanjuan; Zhang, Yuanhu.
Título: The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
Fonte: Biol. Res;49:1-5, 2016. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: BACKGROUND: α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. RESULTS: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O2._) had higher levels in transgenics compared to controls. Transgenic plants also had enhanced sensitivity to oxidative stress. The transcriptome of 8-week-old plants was studied to detect molecular changes. Differentially expressed unigene analysis showed that ubiquitin-mediated proteolysis, cell growth, and death unigenes were upregulated. Unigenes related to photosynthesis, antioxidant activity, and nitrogen metabolism were downregulated. Combined with the expression analysis of senescence marker genes, these results indicate that senescence started in the leaves of the transgenic plants at the vegetative growth stage. CONCLUSIONS: The antioxidative defense system was compromised and the accumulation of reactive oxygen species (ROS) played an important role in the premature aging of transgenic plants.
Descritores: Tabaco/fisiologia
Plantas Geneticamente Modificadas/fisiologia
Antioxidantes/fisiologia
-Fotossíntese/fisiologia
Sesquiterpenos/análise
Sesquiterpenos/metabolismo
Fatores de Tempo
Tabaco/genética
Marcadores Genéticos
Expressão Gênica/fisiologia
Plantas Geneticamente Modificadas/genética
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Superóxidos/análise
Superóxidos/metabolismo
Folhas de Planta/fisiologia
Estresse Oxidativo/fisiologia
Regulação da Expressão Gênica de Plantas/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Peróxido de Hidrogênio/análise
Peróxido de Hidrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central



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