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Texto completo SciELO Chile
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Id: lil-676826
Autor: Pan, Qunwen; Chen, Meizhen; Li, Juan; Wu, Yan; Zhen, Chao; Liang, Bin.
Título: Antitumor function and mechanism of phycoerythrin from Porphyra haitanensis
Fonte: Biol. Res;46(1):87-95, 2013. ilus.
Idioma: en.
Projeto: Industry-University-Research Collaboration program of Guangdong Province. Industry-University-Research Collaboration program of Guangdong Province; . Science and Technology Program of Guangdong Province and Shantou City.
Resumo: The anti-tumor effect of R-Phycoerythrin (R-PE) from Porphyra haitanensis was studied using cell line HeLa as an in vitro model and Sarcoma-180 (S180) tumor-bearing mice as an in vivo model. The results showed that the combination treatment of R-PE and photodynamic therapy PDT) significantly inhibited the growth of HeLa cells up to 81.5%, with a fair dose-effect relationship, but did not inhibit endothelial cells. The annexin v-fitc/PI fluorescence staining experiments demonstrated that at doses between 0~60µg/mL, apoptosis cells and later stage apoptosis cells or necrosis cells increased significantly as the R-PE dosage increased. DNA electrophoresis showed that after R-PE+PDT treatment of HeLa cells for 24 hours, a light "smear" band between 100~400bp appeared to indicate the degradation of genomic DNA. The QRT-PCR results showed that R-PE+PDT treatment increased caspase-3 and caspase-10 gene expression and decreased the Bcl-2 gene expression level significantly as the R-PE dose increased, implying that R-PE promoted HeLa cell apoptosis. Compared with untreated S180 tumor-bearing mice, R-PE injection significantly inhibited the growth of S180 in tumor-bearing mice up to 41.3% at a dose of 300mg-kg-1. Simultaneously, the significant increase of superoxide dismutase (SOD) activity in serum (p < 0.01) and the decrease of the malondialdehyde (MDA) level in liver suggests that R-PE improved the anti-oxidant ability of the S180 tumor-bearing mice, which may related to its antitumor effect. In addition, the R-PE caused a significant increase (p < 0.05) in the spleen index and thymus index, and a significant increase (p < 0.01) in lymphocyte proliferation, NK cell kill activity and the TNF-α level in the serum of S180 tumor-bearing mice. These results strongly suggest that the antitumor effect of R-PE from Porphyra haitanensis functioned by increasing the immunity and antioxidant ability of S180 tumor-bearing mice, promoting apoptosis by increasing protease gene expression and TNF-α secretion.
Descritores: Antineoplásicos Fitogênicos/administração & dosagem
Células HeLa/efeitos dos fármacos
Ficoeritrina/administração & dosagem
/tratamento farmacológico
SARCOMA ACROMION/tratamento farmacológico
-Apoptose/efeitos dos fármacos
Células Matadoras Naturais/efeitos dos fármacos
Peso Molecular
Ficoeritrina/isolamento & purificação
Preparações de Plantas/administração & dosagem
Superóxido Dismutase/farmacologia
Limites: Animais
Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Chile
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Id: lil-567086
Autor: Takahashi, Megumu; Uji, Toshiki; Saga, Naotsune; Mikami, Koji.
Título: Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis
Fonte: Electron. j. biotechnol;13(2):8-9, Mar. 2010. ilus, tab.
Idioma: en.
Projeto: Grants-in-Aid for Scientific Research. Knowledge Cluster Initiative.
Resumo: Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere; however, these protoplasts did not develop. In contrast, protoplasts prepared from gametophytes treated with allantoin were normally developed, in which the overexpression of a â-glucuronidase reporter gene had no effect on the regeneration of protoplasts. Therefore, the use of allantoin in protoplast preparation sheds a new light on the realization of an efficient isolation and selection of study transformed cells from gametophytic blades.
Descritores: Alantoína/fisiologia
Expressão Gênica
Células Germinativas
Folhas de Planta/genética
Responsável: CL1.1 - Biblioteca Central

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