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Pesquisa : B01.650.940.150.469 [Categoria DeCS]
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Texto completo SciELO Brasil
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Id: biblio-1132245
Autor: Giraldo, Néstor David; Buchelly, Raquel Juliana Romo; Hincapié, Danilo Echeverri; Atehortua, Lucia.
Título: Transformation of Brewery Subproducts into Valuable Biomass Using Mixotrophic Culture of Chlorella pyrenoidosa and Associated Bacteria
Fonte: Braz. arch. biol. technol;63:e20190229, 2020. tab, graf.
Idioma: en.
Projeto: Departamento Administrativo de Ciencia, Tecnología e Innovación - Colciencias, Cervecería Union, Fondo de Internacionalización of Universidad de Antioquia and ERANet-LAC1st Joint Call.
Resumo: Abstract To develop a biorefinery concept applied in the brewery industry, Chlorella pyrenoidosa and a consortium of associated bacteria were cultivated mixotrophically in a continuous photobioreactor using brewery low-value subproducts as an integrative process. Beer production residues were biochemically characterized to assess the most promising options to be used as a nutrient source for microalgal cultivation. Due to its physical and chemical properties, pre-treated weak wort was used to prepare an organic complex culture medium for microalgal biotransformation. Filtration and nitrogen supplementation were necessary to improve nutrient removal and biomass productivity. Maximal removal of nitrate and phosphate obtained were 90% and 100% respectively. Depending on operation conditions, total carbohydrates depuration ranged from 50 - 80%. The initial concentration of total carbohydrates of the weak wort must be adjusted to 2 - 4g/L to maintain a stable equilibrium between microalgal and bacterial growth. The biochemical composition of produced biomass varied depending on the cultivation conditions as well as on its final use. Upon continuous mixotrophic conditions evaluated in this study, C. pyrenoidosa was composed mainly of carbohydrates and protein.
Descritores: Cerveja
Fenômenos Bioquímicos
Biotransformação
Chlorella/crescimento & desenvolvimento
Microalgas/crescimento & desenvolvimento
-Carboidratos
Chlorella/química
Biomassa
Fotobiorreatores/microbiologia
Limites: Animais
Responsável: BR1.1 - BIREME


  2 / 22 LILACS  
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Id: biblio-1132214
Autor: Rosa, Ana Priscila Centeno da; Moraes, Luiza; Morais, Etiele Greque de; Costa, Jorge Alberto Vieira.
Título: Fatty Acid Biosynthesis from Chlorella in Autotrophic and Mixotrophic Cultivation
Fonte: Braz. arch. biol. technol;63:e20180534, 2020. tab.
Idioma: en.
Resumo: Abstract Microalgae are photosynthetic microorganisms whose composition and biomass production can be influenced by manipulating the cultivation conditions employed. However, few studies have evaluated the effects of various cultivation conditions in autotrophic and mixotrophic conditions. The present work aimed to evaluate the effects of cultivation conditions on the cell growth and biosynthesis of fatty acids (FAs) by microalgae of the genus Chlorella in autotrophic and mixotrophic cultivation. Evaluation of the effects of the conditions was performed using an experimental design methodology. The highest values of maximum biomass concentration (Xmax) and maximum biomass productivity (Pmax) were obtained in autotrophic cultures. Palmitic acid was the FA obtained at the highest concentration in both cultivation modes. The concentrations of polyunsaturated FAs (PUFAs) ranged from 12.2 to 41.2% in autotrophic cultures and from 11 to 34.3% in the mixotrophic cultures. The variables photoperiod and sodium bicarbonate concentration showed the greatest influence on the Xmax, Pmax, and PUFA concentration in autotrophic and mixotrophic cultivations, respectively. This study verified that the selection of conditions and mode of cultivation contribute to the production of microalgal biomass and FA biosynthesis.
Descritores: Chlorella/metabolismo
Ácidos Graxos/biossíntese
-Chlorella/crescimento & desenvolvimento
Meios de Cultura
Responsável: BR1.1 - BIREME


  3 / 22 LILACS  
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Id: biblio-1053195
Autor: Olasehinde, Tosin A; Odjadjare, Ejovwokoghene C; Mabinya, Leonard V; Olaniran, Ademola O; Okoh, Anthony I.
Título: Chlorella sorokiniana and Chlorella minutissima exhibit antioxidant potentials, inhibit cholinesterases and modulate disaggregation of ß-amyloid fibrils
Fonte: Electron. j. biotechnol;40:1-9, July. 2019. tab, graf, ilus.
Idioma: en.
Resumo: BACKGROUND: Microalgae are aquatic chlorophyll-containing organisms comprising unicellular microscopic forms, and their biomasses are potential sources of bioactive compounds, biofuels and food-based products. However, the neuroprotective effects of microalgal biomass have not been fully explored. In this study, biomass from two Chlorella species was characterized, and their antioxidant, anticholinesterase and anti-amyloidogenic activities were investigated. RESULTS: GC­MS analysis of the extracts revealed the presence of some phenols, sterols, steroids, fatty acids and terpenes. Ethanol extract of Chlorella sorokiniana (14.21 mg GAE/g) and dichloromethane extract of Chlorella minutissima (20.65 mg QE/g) had the highest total phenol and flavonoid contents, respectively. All the extracts scavenged 2,2-diphenyl-1-picrylhydrazyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and hydroxyl radicals. The highest metal chelating activity of the extracts was observed in the ethanol extracts of C. minutissima (102.60 µg/mL) and C. sorokiniana (107.84 µg/mL). Furthermore, the cholinesterase inhibitory activities of the extracts showed that ethanol extract of C. sorokiniana (13.34 µg/mL) exhibited the highest acetylcholinesterase inhibitory activity, while dichloromethane extract of C. minutissima (11.78 µg/mL) showed the highest butyrylcholinesterase inhibitory activity. Incubation of the ß-amyloid protein increased the aggregation of amyloid fibrils after 96 h. However, ethanol extract of C. sorokiniana and C. minutissima inhibited further aggregation of Aß1­42 and caused disaggregation of matured protein fibrils compared to the control. This study reveals the modulatory effects of C. sorokiniana and C. minutissima extracts on some mediators of Alzheimer's disease and provides insights into their potential benefits as functional food, nutraceutics or therapeutic agent for the management of this neurodegenerative disease.
Descritores: Chlorella/química
Inibidores da Colinesterase/farmacologia
Peptídeos beta-Amiloides/antagonistas & inibidores
Antioxidantes/farmacologia
-Fenóis/análise
Esteroides/análise
Esteróis/análise
Terpenos/análise
Extratos Vegetais/farmacologia
Extratos Vegetais/química
Inibidores da Colinesterase/química
Espectroscopia de Infravermelho com Transformada de Fourier
Fármacos Neuroprotetores
Biomassa
Etanol
Ácidos Graxos/análise
Microalgas
Doença de Alzheimer/prevenção & controle
Amiloide/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Antioxidantes/química
Responsável: CL1.1 - Biblioteca Central


  4 / 22 LILACS  
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Id: biblio-855528
Autor: Bontempo, Márcio.
Título: Uso da clorela em odonto-estomatologia / Use of clorela in stomatology
Fonte: Rev. Assoc. Paul. Cir. Dent;47(4):1089-92, jul.-ago. 1993.
Idioma: pt.
Descritores: Chlorella
Clorófitas
Terapias Complementares/estatística & dados numéricos
Estômago/fisiopatologia
Limites: Humanos
Responsável: BR97.1 - Serviço de Documentação Odontológica


  5 / 22 LILACS  
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Texto completo SciELO Costa Rica
Texto completo
Id: lil-659562
Autor: Morales-Ventura, Jesús; Nandini, S.; Sarma, S.S.S; Castellanos-Páez, Maria Elena.
Título: Demography of zooplankton (Anuraeopsis fissa, Brachionus rubens and Moina macrocopa) fed Chlorella vulgaris and Scenedesmus acutus cultured on different media
Fonte: Rev. biol. trop;60(3):955-965, Sept. 2012. ilus, graf.
Idioma: en.
Resumo: Generally zooplankton growth is often limited by the quality of their algal diet. A cheaper common practice in aquaculture, is to culture algae with fertilizers; however, the demography of zooplankton when fed these algae has not yet been evaluated. We studied the population growth and life table demography of the rotifers Anuraeopsis fissa and Brachionus rubens, and the cladoceran Moina macrocopa. For this, the algae Scenedesmus acutus or Chlorella vulgaris were cultured on defined (Bold’s basal) medium or the commercial liquid fertilizer (Bayfolan). Experiments were conducted at one algal concentration 1.0x10(6)cells/mL of C. vulgaris or its equivalent dry weight of 0.5x10(6)cells/mL of S. acutus. The population dynamics were tested at 23±1ºC in 100mL transparent jars, each with 50mL of the test medium, with an initial density of 0.5indiv/mL, for a total of 48 test jars (3 zooplankton 2 algal species x 2 culture media x 4 replicates). For the life table experiments with M. macrocopa, we introduced 10 neonates (<24h old) into each test jar containing the specific algal type and concentration. For the rotifer experiments, we set 5mL tubes with one neonate each and 10 replicates for each algal species and culture medium. We found that the average rotifer life span was not influenced by the diet, but for M. macrocopa fed S. acutus cultured in Bold’s medium, the average lifespan was significantly lower than with the other diets. The gross and net reproductive rates of A. fissa (ranging from 18-36 offspring per female) were significantly higher for C. vulgaris cultured in Bold medium. Regardless of the culture medium, Chlorella resulted in significantly higher gross and net reproductive rates for B. rubens than S. acutus diets. The reproductive rates of M. macrocopa were significantly higher in all the tested diets except when fed with S. acutus in Bold medium. The population increase rate, derived from growth experiments of A. fissa and B. rubens, ranged from 0.1-0.25/d and were significantly higher on C. vulgaris cultured in liquid fertilizer as compared to the other diets. The growth rates of M. macrocopa ranged from 0.1 to 0.38/d, and were highest with diets of C. vulgaris cultured in Bold medium and S. acutus cultured in fertilizer. Thus, regardless of the culture medium used, the growth rates of the evaluated zooplankton species were higher with Chlorella than with Scenedesmus. The peak population density was highest (2 800ind/mL) for A. fissa fed Chlorella that was cultured on liquid fertilizers, while B. rubens and M. macrocopa had peak abundances of 480 and 12ind/mL, respectively under similar conditions.

Generalmente el crecimiento del zooplancton está a menudo limitado por la calidad de su dieta de algas. La demografía del zooplancton durante la alimentación con algas no ha sido estudiada, a pesar de que el cultivo de algas con fertilizantes es una práctica económica común en acuacultura. Se analizó la demografía de Anuraeopsis fissa y Brachionus rubens (rotíferos) y Moina macrocopa (cladóceros), alimentados con las algas verdes Scenedesmus acutus o Chlorella vulgaris cultivadas en medio Bold o fertilizante líquido comercial (Bayfolan, de Bayer). En los rotíferos no se observaron diferencias significativas en el promedio de vida, sin embargo, este parámetro en M. macrocopa con S. acutus cultivada en Medio Bold, fue significativamente menor que en otras dietas. Las tasas de reproducción bruta y neta de A. fissa fueron significativamente mayores con C. vulgaris cultivada en medio Bold, que con el fertilizante; estas tasas en B. rubens, independientemente del medio de cultivo, resultaron significativamente mayores con Chlorella que S. acutus. La tasa de reproducción de M. macrocopa fue significativamente mayor en todas, a excepción de S. acutus en Bold. En el crecimiento poblacional con A. fissa y B. rubens la tasa de crecimiento poblacional varió de 0.1 hasta 0.25/d, significativamente mayores en C. vulgaris cultivadas con fertilizante, en comparación con las otras dietas; en M. macrocopa la tasa de crecimiento varió desde 0.1 hasta 0.38/d, las más altas fueron: con C. vulgaris cultivadas en medio Bold y S. acutus cultivadas con fertilizante. Así, en todas las especies, la tasa de crecimiento fue más alta con Chlorella que con Scenedesmus.
Descritores: Cladóceros/fisiologia
Rotíferos/fisiologia
-Chlorella
Cladóceros/classificação
Dieta
Fertilizantes
Tábuas de Vida
Crescimento Demográfico
Reprodução/fisiologia
Rotíferos/classificação
Scenedesmus
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Costa Rica
Texto completo
Id: lil-637854
Autor: Cabrera F, María I.
Título: Tasa de crecimiento poblacional del rotífero Brachionus rotundiformis (Rotifera: Brachionidae) en un quimiostato de dos cámaras
Fonte: Rev. biol. trop;56(3):1149-1157, sep. 2008. graf, tab.
Idioma: es.
Resumo: Population growth rate of the rotifer Brachionus rotundiformis (Rotifera: Brachionidae) in two-stage chemostat. The population growth rates of Brachionus rotundiformis were estimated in two-stage chemostat cultures. Chlorella sorokiniana was supplied continuously from a steady state culture growing with constant illumination on limiting nitrate. Rotifer growth in the second stage was limited by the rate of algal supply. The algal supply rate and rotifer population growth rate were determined by the second-stage dilution rate. The maximum population growth rate in the transient state of B. rotundiformis (1.96 day-1) was observed at 2.5 x 106 cel/ml of the algae whereas in the steady state the maximum population growth rate (1.09 day-1) was similar to the point Hopf's bifurcation predicted by Fussmann and was observed at 1 x 106 cel/ml of the algae. In the transient state, the rotifer's growth rate increased and the duplication time decreased at higher algal concentrations, until reaching a peak where the population growth rate begins to decrease. In the steady state, the opposite was true. The growth rates observed in this work are among the highest recorded for this rotifer in continuous cultures. Rev. Biol. Trop. 56 (3): 1149-1157. Epub 2008 September 30.

Se estimaron las tasas de crecimiento poblacionales de Brachionus rotundiformis en un quimiostato de dos cámaras. Suministramos Chlorella sorokiniana continuamente a partir de un cultivo en estado estacionario con iluminación constante y nitrato como nutriente limitante. El crecimiento del rotífero en la segunda cámara estuvo limitado por la tasa de suministro del alga. La tasa de suministro del alga y la tasa de crecimiento poblacional del rotífero fueron determinadas a partir de la tasa de dilución en la segunda cámara. La tasa máxima poblacional de crecimiento en estado de transición de B. rotundiformis (1.96 día-1) se obtuvo a 2.5 x106 cel/ml del alga, mientras que en estado estacionario la tasa máxima (1.09 día-1) fue similar al punto de bifurcación de Hopf predicho en Fussmann et al., (2000) y se obtuvo a 1 x106 cel/ml del alga. En la fase de transición se observó que a mayor concentración del alga, mayor era la tasa de crecimiento del rotífero y menor su tiempo de duplicación, hasta alcanzar un pico donde comienza a decrecer. En la fase estacionaria se observó lo contrario. Los valores obtenidos se encuentran entre los más altos reportados hasta ahora para este rotífero en cultivos continuos.
Descritores: Chlorella
Abastecimento de Alimentos
Rotíferos/crescimento & desenvolvimento
-Ambiente Controlado
Densidade Demográfica
Crescimento Demográfico
Rotíferos/fisiologia
Fatores de Tempo
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Chile
Texto completo
Id: lil-522201
Autor: Travieso Córdoba, Lissette; Domínguez Bocanegra, Alma Rosa; Rincón Llorente, Bárbara; Sánchez Hernández, Enrique; Benítez Echegoyen, Francisco; Borja, Rafael; Raposo Bejines, Francisco; Colmenarejo Morcillo, Manuel Francisco.
Título: Batch culture growth of Chlorella zofingiensis on effluent derived from two-stage anaerobic digestion of two-phase olive mill solid waste
Fonte: Electron. j. biotechnol;11(2):12-19, Apr. 2008. ilus, graf, tab.
Idioma: en.
Resumo: This paper presents the use of an effluent derived from two-stage anaerobic digestion of two-phase olive mill solid waste (OMSW) as a substrate for the production of Chlorella zofingiensis in batch mode. Chlorella zofingiensis when grown autotrophycally can accumulate significant quantities of valuable carotenoids which are used as an additive in fish and poultry farming, as colorants in foods and in health care products. It was found that two-phase OMSW previously treated by two-stage anaerobic digestion and further sterilized may be used as a culture medium for the microalgae Chlorella zofingiensis. Typical growth curves were obtained using both the above-mentioned anaerobic effluent and a synthetic medium. Total chemical oxygen demand (TCOD) and soluble chemical oxygen demand (SCOD) removals of 37 percent and 45 percent respectively were achieved in batch experiments after 11 days' operation time. The specific growth rate was lower when the treated effluent was used as the feed substrate (0.02 h-1) in comparison to the synthetic medium (0.03 h-1). The specific growth rates of the exponential phases were determined by using a first-order kinetic model applied to chlorophyll a (Ca) and total chlorophyll (TC) concentrations, as indirect measurements of the microalgae concentration. It was concluded that the effluent from two-stage anaerobic digestion of two-phase OMSW constituted an appropriate culture medium for the growth of Chlorella zofingiensis, providing a simple technology feasible for producing a very useful product for animal feeding.
Descritores: Digestão Anaeróbia
Chlorella
Resíduos Sólidos
Gorduras Vegetais
-Carotenoides
Cinética
Espectrofotometria
Responsável: CL1.1 - Biblioteca Central


  8 / 22 LILACS  
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Id: lil-480275
Autor: Rosales Loaiza, Néstor; Bermúdez, José; Moronta, Reyna; Morales, Ever.
Título: Gallinaza: un residual avícola como fuente alternativa de nutrientes para producción de biomasa microalgal / Chicken manure: poultry waste as an alternative nutrient source for microalgal biomass production
Fonte: Rev. colomb. biotecnol;9(1):41-48, jul. 2007. tab, graf.
Idioma: es.
Resumo: La gallinaza puede ser usada como una fuente alternativa de nutrientes para el cultivo de microalgas, proveyendo de biomasa microalgal utilizable como producto final. El objetivo de este trabajo fue evaluar el efecto de la fracción soluble de gallinaza (FSG) a tres diferentes concentraciones (6, 18 y 36 por cien)sobre el crecimiento, la producción de pigmentos y proteínas de la microalga marina Chroomonas sp. y de la microalga de agua dulce Chlorella sorokiniana. La FSG no biodegradada mostró un efecto letal sobre el crecimiento de ambas microalgas. La FSG tratada aeróbicamente mejoró el crecimiento de Chroomonas sp. a 18 por cien con 131,37+-13,66x10 a la 6 cel mL-1, y a 36 por cien para C. sorokiniana de 228,64+-4,90x10 a la 6 cel mL-1 (p<0,05). De igual forma, el peso seco más alto se obtuvo a 18 y 36 por cien para Chroomonas y C. sorokinianade 1,69+-0,03 y 1,07+-0,01 mg mL-1, respectivamente (p<0,05). La producción de pigmentos fue más alta a 36 por cien de FSG. Los máximos valores de clorofila fueron de 21,49+-2,51 y 16,06+-0,83 ug mL-1, y de carotenoides de 12,35+-1,69 y 1,96+-0,19 ug mL-1 para Chroomonas y C. sorokiniana, respectivamente. La máxima producción de proteínas fue alcanzada a 18 por cien para Chroomonas sp con 384,54+-18,52 ug mL-1,y a 36 por cien para C. sorokiniana con 779,53+-11,14 ug mL-1 (p<0,05). Estos resultados sugieren que la fracción soluble de gallinaza puede ser usada como una fuente de nutrientes para la producción de biomasa enriquecida con pigmentos y proteínas, reduciendo así costos de cultivo.
Descritores: Biomassa
Chlorella
Eucariotos
Pigmentos Biológicos
Proteínas
Tipo de Publ: Estudo de Avaliação
Estudo Comparativo
Responsável: CO326 - Departamento de Biología


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Id: lil-463230
Autor: Quintana Cabrales, María Magdalena; Fernández González, Manuel.
Título: Utilización de residual aviar como fuente de nutrientes en cultivos de microalgas / Use of aviary residual as a source of nutrients in microalgas cultures
Fonte: Medisan;8(3), jul.-sept. 2004.
Idioma: es.
Resumo: El residual aviar, como todos los desechos animales, es portador de variados elementos químicos, que tratados adecuadamente pueden ser utilizados como fuente de nutrientes para cultivos de microalgas. Se caracteriza con la determinación de la demanda química de oxígeno, demanda bioquímica de oxígeno, nitrógeno, fósforo, potasio y magnesio como parámetros de interés para la investigación. El residual se trabaja en 3 formas diferentes: excretas secas, excretas frescas, sobrenadante de una disolución de excretas secas y sobrenadante de una disolución de excretas frescas. Los resultados mostraron que la forma más adecuada para usar este residual como fuente de nutrientes para el cultivo de Chlorella vulgaris, es el sobrenadante de la disolución de excretas secas al 20 por ciento, por cuanto aporta los valores promedios siguientes: DQO: 979,84 mg/L; N total: 876,24; Mg: 74,25; K: 142,27 y P total: 733,12 mg/L. La biomasa resultante de la producción de biomasa de Chlorella vulgaris con este residual contenía: 39,8 por ciento de proteínas, 6,49 por ciento de grasa, 36,22 por ciento de carbohidratos, 5,27 por ciento de cenizas y 1,27 por ciento de fibra
Descritores: Chlorella
Eucariotos
Resíduos
Responsável: CU418.1 - Centro Provincial de Información de Ciencias Médicas de Santiago de Cuba


  10 / 22 LILACS  
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Id: lil-386902
Autor: Morris Quevedo, Humberto Juaquín; Borges Quintana, Leonardo; Martínez Manrique, Clara Esther; Carrillo Farnés, Olimpia.
Título: Restauración de la inmunocompetencia en ratones malnutridos con la administración de un hidrolizado de microalgas / Restoration of the immunocompetence in malnourished mice by the administration of a microalgae hydrolysate
Fonte: Rev. cuba. farm;37(3), sept.-dic. 2003. tab, graf.
Idioma: es.
Resumo: Se evaluó el efecto de la administración intraperitoneal de un hidrolizado proteico de la microalga Chlorella vulgaris en una dosis de 500 mg/kg de peso durante 6 días, como complemento de la dieta convencional en la recuperación de la inmunocompetencia de ratones Balb/c con malnutrición proteico-energética inducida experimentalmente por restricción dietética. La intervención con el hidrolizado implicó la restauración del conteo de leucocitos totales a valores similares a los del grupo control; a un incremento en los niveles de linfocitos en sangre periférica; estimuló la proliferación de las células del sistema fagocítico mononuclear y el metabolismo de los macrófagos, expresado por una mayor actividad de la enzima fosfatasa ácida lisosomal y al aumento del peso relativo y actividad hematopoyética del bazo. Estas acciones moduladoras permiten considerar al producto como un preparado inmunológicamente activo capaz de inducir un estado incrementado de resistencia no específica en el organismo
Descritores: Animais de Laboratório
Chlorella
Desnutrição Proteico-Calórica/tratamento farmacológico
Imunocompetência
Camundongos Endogâmicos BALB C
Limites: Camundongos
Responsável: CU1.1 - Biblioteca Médica Nacional



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