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Id: biblio-907531
Autor: Fantinelli, Juliana; González-Arbeláez, Luisa; Ciocci-Pardo, Alejandro; Schinella, Guillermo; Mosca, Susana M.
Título: Comparative effects of natural products on ischemia-reperfusion injury: relation to their "in vitro" antioxidant capacity / Efectos comparativos de productos naturales sobre la injuria por isquemia-reperfusión: relación con la capacidad antioxidante ¨in vitro¨
Fonte: Bol. latinoam. Caribe plantas med. aromát;15(3):151-163, May. 2016. tab, graf, ilus.
Idioma: en.
Projeto: National University of La Plata.
Resumo: Our aim was to compare the effects of a non-alcoholic Cabernet-Sauvignon (CS), Malbec (M), Merlot blend (BW) red wine extracts, Ilex paraguariensis (Ip) or Ilex brasiliensis (Ib) aqueous extracts, Vaccinium meridionale Swartz (mortiño) fermented extract (FE), berry juice (BJ) and polyphenols-riched fractions of cocoa(PFC) against reperfusion injury. Isolated rat hearts were submitted to 20 min of global ischemia (GI) and 30 min of reperfusion (R). Other hearts were treated 10 min before GI and first 10 min of R with the extracts. CS, M, Ip, Ib and FE attenuated the myocardial dysfunction and oxidative damage whereas BW, BJ and PFC were ineffective. Paradoxically, PFC had the highest and BW similar scavenging activity than protective extracts. The beneficial actions were lost when nitric oxide synthase (NOS) was inhibited. These data indicate that in vitro antioxidant capacity of natural products is not primarily responsible for the cardioprotection being involved NO-dependent pathways.

Nuestro objetivo fue comparar los efectos de extractos no alcohólicos de los vinos tinto Cabernet-Sauvignon (CS), Malbec (M) y Merlot (BW), de extractos acuosos de Ilex paraguariensis (Ip) e Ilex brasiliensis (Ib), de un extracto fermentado (FE) de Vaccinium meridionale Swartz (mortiño), del jugo del mortiño (BJ) y de fracciones enriquecidas en polifenoles de cacao (PFC) sobre las alteraciones miocárdicas producidas por isquemia-reperfusión. Para ello, corazones aislados de rata fueron sometidos a 20 min de isquemia global (GI) y 30 min de reperfusión (R). Otros corazones fueron tratados 10 minutos antes de GI y durante los primeros 10 minutos de la R con los extractos. CS, M, Ip, Ib y FE atenuaron la disfunción contráctil postisquémica y el daño oxidativo mientras que BW, BJ y PFC fueron ineficaces. Paradójicamente, PFC mostró la más alta y BW similar actividad antioxidante que los extractos protectores. Las acciones beneficiosas fueron abolidas cuando la óxido nítrico sintasa (NOS) fue inhibida. Estos datos indican que la capacidad antioxidante in vitro de los productos naturales no es el principal responsable de la cardioprotección estando involucradas vías dependientes del NO.
Descritores: Antioxidantes/uso terapêutico
Flavonoides/uso terapêutico
Fenóis/uso terapêutico
Extratos Vegetais/uso terapêutico
Traumatismo por Reperfusão/tratamento farmacológico
-Western Blotting
Técnicas In Vitro
Óxido Nítrico Sintase
Limites: Animais
Tipo de Publ: Estudo Comparativo
Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central

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Id: lil-541120
Autor: Mroginski, L. A; Sansberro, P. A; Scocchi, A. M; Luna, C; Rey, H. Y.
Título: A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)
Fonte: Biocell;32(1):33-39, Apr. 2008. ilus, tab.
Idioma: en.
Resumo: Tropical Ilex species have recalcitrant seeds. This work describes experiments demonstrating the feasibility of long-term conservation of Ilex brasiliensis, I. brevicuspis, I. dumosa, I. intergerrima, I. paraguariensis, I. pseudoboxus, I. taubertiana, and I. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. The embryos were aseptically removed from the seeds and precultured (7 days) in the dark, at 27 +/- 2 degrees C on solidified (0.8% agar) 1/4MS medium, [consisting of quarter-strength salts and vitamins of Murashige and Skoog (1962) medium] with 3% sucrose and 0.1 mg/l Zeatin.The embryos were then encapsulated in 3% calcium alginate beads and pretreated at 24 h intervals in liquid medium supplemented with progressively increasing sucrose concentrations (0.5, 0.7 5 and 1 M). Beads were dehydrated for 5 h with silicagel to 25% water content (fresh weight basis) and then placed in sterile 5 ml cryovials. Then the beads were either plunged rapidly in liquid nitrogen were they were kept for 1 h (rapid cooling) or cooled at 1 degrees C min(-1) to -30 degrees C. Then the beads were immersed in liquid nitrogen for 1 h (slow cooling). The beads were rewarmed by immersion of the cryovials for 1 min in a water bath thermostated at 30 degrees C. Finally, beads were transferred onto culture medium (1/4MS, 3% sucrose, 0.1 mg/l zeatin, solidified with 0.8% agar) and incubated in a growth room at 27 +/- 2 degrees C under a 14 h light (116 micromol. m(-2) x s(-1))/ 10 h dark photoperiod. Maximum recovery percentages between 15 and 83% (depending on de the species and the treatment) were obtained with the cryopreserved embryos.
Descritores: Sobrevivência Celular
Técnicas de Cultura de Tecidos
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Avaliação
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo

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Id: lil-384242
Autor: Luna, C; Sansberro, P; Mroginski, L; Tarragó, J.
Título: Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Fonte: Biocell;27(2):205-212, Aug. 2003.
Idioma: en.
Resumo: Micropropagation of Ilex dumosa var. dumosa R. ("yerba señorita") from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in 1/4 strength Murashige and Skoog medium (1/4 MS) plus 30 gr x L(-1) sucrose and supplemented with 4.4 microM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr x L(-1) sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in 1/4 MS (30 gr x L(-1) sucrose, 0.25% Phytagel) with 7.3 microM IBA and 2) 21 days in the same medium without IBA and 20 microM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation schedule can be implemented in breeding programs of Ilex dumosa.
Descritores: Adenina/análogos & derivados
Técnicas de Cultura de Células
Ilex/crescimento & desenvolvimento
Meios de Cultura/farmacologia
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/crescimento & desenvolvimento
Ilex/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Regeneração/efeitos dos fármacos
Reguladores de Crescimento de Plantas/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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