Base de dados : LILACS
Pesquisa : B01.650.940.800.575.912.250.093.615 [Categoria DeCS]
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Id: lil-775128
Autor: Alrumman, Sulaiman A..
Título: Enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid
Fonte: Braz. j. microbiol;47(1):110-119, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. In this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from Geobacillus stearothermophilus was optimized. The alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. The maximum rate of saccharification was found at a substrate concentration of 4% and enzyme concentration of 30 FPU/g of substrate. The optimum pH and temperature for the bioconversions were 5.0 and 50 °C, respectively, after 24 h of incubation, with a yield of 31.56 mg/mL of glucose at a saccharification degree of 71.03%. The saccharification was increased to 94.88% by removal of the hydrolysate after 24 h by using a two-step hydrolysis. Significant lactic acid production (27.8 mg/mL) was obtained by separate saccharification and fermentation after 72 h of incubation. The results indicate that production of fermentable sugar and lactic acid is feasible and may reduce environmental pollution by using date palm wastes as a cheap substrate.
Descritores: Celulases/metabolismo
Celulose/metabolismo
Geobacillus stearothermophilus/enzimologia
Glucose/metabolismo
Resíduos Industriais
Ácido Láctico/metabolismo
Phoeniceae/metabolismo
-Álcalis
Biotransformação
Fermentação
Concentração de Íons de Hidrogênio
Phoeniceae/efeitos dos fármacos
Temperatura Ambiente
Responsável: BR1.1 - BIREME


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Id: lil-755836
Autor: Nancib, Aicha; Nancib, Nabil; Boubendir, Abdelhafid; Boudrant, Joseph.
Título: The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp. rhamnosus
Fonte: Braz. j. microbiol;46(3):893-902, July-Sept. 2015. tab, ilus.
Idioma: en.
Resumo:

The production of lactic acid from date juice by Lactobacillus caseisubsp. rhamnosus in batch and fed-batch cultures has been investigated. The fed-batch culture system gave better results for lactic acid production and volumetric productivity. The aim of this work is to determine the effects of the feeding rate and the concentration of the feeding medium containing date juice glucose on the cell growth, the consumption of glucose and the lactic acid production by Lactobacillus casei subsp. rhamnosus in fed-batch cultures. For this study, two concentrations of the feeding medium (62 and 100 g/L of date juice glucose) were tested at different feeding rates (18, 22, 33, 75 and 150 mL/h). The highest volumetric productivity (1.3 g/L.h) and lactic acid yield (1.7 g/g) were obtained at a feeding rate of 33 mL/h and a date juice glucose concentration of 62 g/L in the feeding medium. As a result, most of the date juice glucose was completely utilised (residual glucose 1 g/L), and a maximum lactic acid production level (89.2 g/L) was obtained.

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Descritores: Técnicas de Cultura Celular por Lotes
Ácido Láctico/metabolismo
Lactobacillus casei/metabolismo
Lactobacillus rhamnosus/metabolismo
Phoeniceae/metabolismo
-Fermentação
Extratos Vegetais/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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