Base de dados : LILACS
Pesquisa : B01.650.940.800.575.912. [Categoria DeCS]
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Id: biblio-912233
Autor: Zambrano Zambrano, Jose Paolo.
Título: Determinar el efecto de la fibra de (Brassica rapa L) Nabo silvestre sobre el peso, grasa corporal y proteínas totales en ratas (Rattus novergicus variedad Sprague dawley) con obesidad inducida, Arequipa 2015 / To determine the effect of (Brassica rapa L) fiber on wild weight, body fat and total proteins in rats (Rattus novergicus variety Sprague dawley) with induced obesity, Arequipa 2015.
Fonte: Arequipa; s.n; 2015. 64 p.
Idioma: es.
Tese: Apresentada a Universidad Nacional de San Agustin para obtenção do grau de Especialista.
Resumo: Este estudio fue diseñado para investigar la efectividad de la administración de micropulverizado de Brassica rapa L. (Nabo silvestre) en la disminución de la grasa corporal en ratas con obesidad inducida. Fue un estudio experimental realizado en 30 ratas macho adultas (Rattus norvergicus variedad Sprague Dawley).Los animales experimentales fueron sometidos a 30 días de engorde y posteriormente fueron tratadas con Brassica rapa L. (Nabo Silvestre) con dosis diarias de10, 25 y 50 mg/kg de peso durante 30 días. El porcentaje de grasa, medido utilizando el método Soxhlet, disminuyó significativamente de 18,17 ± 0,61% a 4,23 ± 0,39% en el tratamiento con 10 mg/kg. de peso de Brassica rapa L. (nabo silvestre) con 25 mg/kg. de Brassica rapa L. (nabo silvestre) el promedio fue 8,62 ± 0,46% y en el tratamiento con 50 mg/kg de Brassica rapa L. (nabo silvestre) el promedio fue de 11,50 ± 0,95%.Por lo tanto concluimos que la administración diaria de 10 mg/kg de peso Brassica rapa L. (nabo silvestre) es efectiva para la disminución de porcentaje de grasa corporal y puede ser utilizado como complemento para un programa de reducción de peso y grasa corporal.
Descritores: Brassica rapa
Modelos Animais
Limites: Animais
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Possas, Cristina de Albuquerque
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Id: lil-741621
Autor: Martins, Reinaldo de Menezes; Possas, Cristina de Albuquerque; Homma, Akira.
Título: Historical review of clinical vaccine studies at Oswaldo Cruz Institute and Oswaldo Cruz Foundation - technological development issues
Fonte: Mem. Inst. Oswaldo Cruz;110(1):114-124, 03/02/2015. tab.
Idioma: en.
Resumo: This paper presents, from the perspective of technological development and production, the results of an investigation examining 61 clinical studies with vaccines conducted in Brazil between 1938-2013, with the participation of the Oswaldo Cruz Institute (IOC) and the Oswaldo Cruz Foundation (Fiocruz). These studies have been identified and reviewed according to criteria, such as the kind of vaccine (viral, bacterial, parasitic), their rationale, design and methodological strategies. The results indicate that IOC and Fiocruz have accumulated along this time significant knowledge and experience for the performance of studies in all clinical phases and are prepared for the development of new vaccines products and processes. We recommend national policy strategies to overcome existing regulatory and financing constraints.
Descritores: Ração Animal/efeitos adversos
Proteínas na Dieta/química
Modelos Biológicos
-Brassica rapa/química
Precipitação Química
Proteínas na Dieta/metabolismo
Fabaceae/efeitos adversos
Frutas/efeitos adversos
Estrutura Molecular
Peso Molecular
Concentração Osmolar
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Proantocianidinas/efeitos adversos
Ribulose-Bifosfato Carboxilase/química
Ribulose-Bifosfato Carboxilase/metabolismo
Soroalbumina Bovina/química
Soroalbumina Bovina/metabolismo
Limites: Animais
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
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Id: lil-723123
Autor: Solanki, Manu; Garg, Faquir C..
Título: The use of lacZ marker in enumeration of Azotobacter chroococcum in carrier based inoculants
Fonte: Braz. j. microbiol;45(2):595-601, Apr.-June 2014. tab.
Idioma: en.
Resumo: A transconjugant of Azotobacter chroococcum Mac 27 tagged with lac Z(A. chroococcum Mac27 L) was found to possess high levels of β-galactosidase activity constitutively.Further, the lac Z marker was found to be stably integrated into the chromosome of the A. chroococcum Mac 27 and did not have any adverse effect on growth, nitrogen fixation and excretion of ammonia. A quick method to determine the viable cell number in broth culture and carrier based inoculants has been developed on the basis of β-galactosidase assay. It was found that there was a direct relationship between the number of cell as determined by standard plate count and intensity of colour that developed upon degradation of ONPG due to β-galactosidase activity .The method was found to be sensitive enough to determine 1.7 x 10(6) CFU mL-1 in broth culture as well as carrier based Azotobacter inoculants. Further, it was observed that when A. chroococcum Mac27 L was inoculated on Brassica campestris, it could be detected in the presence of other bacteria capable of growing on Burks agar medium containing X-gal on the basis of lac Z genetic marker.
Descritores: Azotobacter/isolamento & purificação
Carga Bacteriana/métodos
Genes Reporter
-Brassica rapa/microbiologia
Sensibilidade e Especificidade
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME

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Texto completo SciELO Chile
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Id: lil-591899
Autor: Nikolic, Zorica; Vujakovic, Milka; Jeromela, Ana Marjanovic; Jovicic, Dusica.
Título: Implementation of monitoring for genetically modified rapeseed in Serbia
Fonte: Electron. j. biotechnol;13(5):17-18, Sept. 2010. ilus, tab.
Idioma: en.
Projeto: Ministry of Science and Technological Development.
Resumo: Genetically modified (GMO) rapeseed (Brassica napus) is not grown commercially in European Union, but several lines have been approved for production and use as food and feed. A case-specific monitoring of herbicide-tolerant rapeseed, events RT73, RF3 and T45 was established by Ministry of Agriculture of Republic of Serbia. The objectives of the present study were to introduce methods for detection of herbicide-tolerant GM oilseed rape, investigate occurrence and monitor the presence of GM rapeseed in seed and the feed products, as well as to develop a protocol for quantification. The study was based on 48 samples, rapeseed (33) and feed (15) products, imported from EU countries (Germany, Belgium, France, Czech Republic, Austria) and from domestic market. Seven positive feed samples and no positive seed samples have found. The percent of GMO in feed samples, estimated on semi-quantitative way, was below labelling threshold. Adventitious presence of GM materials in non-GM grain, derived food and feedstuffs is a concern to international grain trade and needs continuous monitoring.
Descritores: DNA de Plantas/análise
Brassica rapa/genética
Organismos Geneticamente Modificados
-DNA de Plantas/genética
Reação em Cadeia da Polimerase
Responsável: CL1.1 - Biblioteca Central

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