Base de dados : LILACS
Pesquisa : B01.650.940.800.575.912.250.341.984 [Categoria DeCS]
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Id: lil-734621
Autor: Karimi, Ehsan; Jaafar, Hawa ZE; Ghasemzadeh, Ali; Ebrahimi, Mahdi.
Título: Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth
Fonte: Biol. Res;48:1-6, 2015. graf, tab.
Idioma: en.
Resumo: BACKGROUND: The present study was conducted in order to evaluate the fatty acid profile, anti-oxidant and anti-bacterial activities from the microwave aqueous extract of the leaves of three different varieties of Labisia pumila Benth. RESULTS: The chemical analysis of the extract showed that fatty acids (palmitic, palmitoleic, stearic, oleic, linoleic and α-linolenic) acid as the main components in three varieties of L. pumila leaves. Furthermore, the obtained results of the anti-oxidant revealed that L. pumila var. alata contained higher anti-oxidative activities compared to var. pumila and var. lanceolata. However, these values were lower than the tested anti-oxidant standards. On the other hand, the aqueous leaf extracts in all three varieties of L. pumila were also found to inhibit a variable degree of antibacterial activities against eight bacteria (four Gram-positive and four Gram-negative bacteria). CONCLUSIONS: In this study, it was observed the leaves of three varieties of L. pumila exhibited variable patterns of fatty acids and the microwave aqueous extraction possess anti-oxidant and anti-bacterial activities.
Descritores: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Ácidos Graxos/análise
Micro-Ondas
Primulaceae/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
-Benzotiazóis/metabolismo
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Flavonoides/análise
Sequestradores de Radicais Livres/análise
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
INHIBITORY CONCENTRATION ACADEMIES AND INSTITUTES
Primulaceae/classificação
Óxido Nítrico/metabolismo
Fenóis/análise
Folhas de Planta/química
Ácidos Sulfônicos/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-591930
Autor: Hoenemann, Claudia; Hohe, Annette.
Título: Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
Fonte: Electron. j. biotechnol;14(1):12-13, Jan. 2011. ilus, tab.
Idioma: en.
Projeto: DFG.
Resumo: As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools 'geNorm' and 'NormFinder'. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). 'NormFinder' as well as 'geNorm' identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes differed depending on the algorithm. According to 'NormFinder' COG complex component displayed the most stable expression whereas 'geNorm' indicated V-ATPase G subunit 1 and a putative ABC transporter ATPase to be the most reliable reference genes. Hence, we concluded to use a normalization factor calculated from the four reference genes V-ATPase G subunit 1, ABC transporter ATPase, Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4) and COG complex component for normalization of qRT-PCR in cell cultures of Cyclamen persicum.
Descritores: Cyclamen
Desenvolvimento Embrionário
Desenvolvimento Embrionário/genética
-Primulaceae/química
Primulaceae/ultraestrutura
Reação em Cadeia da Polimerase/métodos
Tipo de Publ: Relatório Técnico
Responsável: CL1.1 - Biblioteca Central



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